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https://www.readbyqxmd.com/read/29772702/exploration-of-aba-responsive-mirnas-reveals-a-new-hormone-signaling-crosstalk-pathway-regulating-root-growth-of-populus-euphratica
#1
Conglong Lian, Kun Yao, Hui Duan, Qing Li, Chao Liu, Weilun Yin, Xinli Xia
Abscisic acid (ABA) plays an important role in the regulation of plant adaptation, seed germination, and root development in plants. However, the mechanism of ABA regulation of root development is still poorly understood, especially through the miRNA-mediated pathway . Here, small RNA (sRNA)-seq and degradome-seq were used to analyze the miRNAs' responsive to ABA in the stems and roots of P. euphratica , a model tree species for abiotic stress-resistance research. In total, 255 unique mature sequences, containing 154 known miRNAs and 101 novel miRNAs were identified, among which 33 miRNAs and 54 miRNAs were responsive to ABA in the roots and stems, respectively...
May 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29764387/genome-wide-identification-and-comprehensive-analysis-of-micrornas-and-phased-small-interfering-rnas-in-watermelon
#2
Li Liu, Shuchao Ren, Junqiang Guo, Qingyi Wang, Xiaotuo Zhang, Peiran Liao, Shipeng Li, Ramanjulu Sunkar, Yun Zheng
BACKGROUND: MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs involved in the post-transcriptional gene regulation and play a critical role in plant growth, development and stress responses. Watermelon (Citrullus lanatus L.) is one of the important agricultural crops worldwide. However, the watermelon miRNAs and phasiRNAs and their functions are not well explored. RESULTS: Here we carried out computational and experimental analysis of miRNAs and phased small interfering RNAs (phasiRNAs) in watermelon by analyzing 14 small RNA profiles from roots, leaves, androecium, petals, and fruits, and one published small RNA profile of mixed tissues...
May 9, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29739311/transcriptome-analysis-reveals-new-micrornas-mediated-pathway-involved-in-anther-development-in-male-sterile-wheat
#3
Longqing Sun, Genlou Sun, Chenxia Shi, Dongfa Sun
BACKGROUND: 337S is a novel bi-pole-photo-thermo-sensitive genic male sterile line in wheat, and sensitive to both long day length/high temperature and short day length/low temperature condition. Although the regulatory function of MicroRNAs (miRNAs) in reproductive development has been increasingly studied, their roles in pre-meiotic and meiotic cells formation of plants have not been clearly explored. Here, we explored the roles of miRNAs in regulating male sterility of 337S at short day length/low temperature condition...
May 8, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29681080/identification-and-characterisation-of-a-previously-unknown-drought-tolerance-associated-microrna-in-barley
#4
Hui Zhou, Syed Sarfraz Hussain, Michael Hackenberg, Natalia Bazanova, Omid Eini, Jie Li, Perry Gustafson, Bujun Shi
Drought is the most serious abiotic stress, which causes crop losses on worldwide scale. The present study identified a previously unknown microRNA (designated as hvu-miRX) of 21 nucleotides (nt) in barley. Its precursor (designated pre-miRX) and primary transcript (designated pri-miRX) were also identified, with lengths of 73 nt and 559 nt, respectively. The identified upstream sequence of pri-miRX contains both the TATA box and the CAAT box, which are both required for transcription initiation. Transient promoter activation assays showed that the core promoter region of pri-miRX ranged 500 nt from the transcription start site...
April 22, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29662498/small-rna-and-degradome-deep-sequencing-reveals-the-roles-of-micrornas-in-seed-expansion-in-peanut-arachis-hypogaea-l
#5
Xingli Ma, Xingguo Zhang, Kunkun Zhao, Fapeng Li, Ke Li, Longlong Ning, Jialin He, Zeyu Xin, Dongmei Yin
Seed expansion in peanut is a complex biological process involving many gene regulatory pathways. MicroRNAs (miRNAs) play important regulatory roles in plant growth and development, but little is known about their functions during seed expansion, or how they contribute to seed expansion in different peanut lines. We examined seed miRNA expression patterns at 15 and 35 days after flowering (DAF) in two peanut eighth-generation recombinant inbred lines (RIL8); 8106, a medium-pod variety, and 8107, a super-pod variety...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29621707/identification-of-cd-responsive-rna-helicase-genes-and-expression-of-a-putative-bnrh-24-mediated-by-mir158-in-canola-brassica-napus
#6
Xian Duo Zhang, Jia Yun Sun, Yuan Yuan You, Jian Bo Song, Zhi Min Yang
RNA helicases play crucial roles in RNA splicing, transport, editing and degradation, protein translation initiation and siRNA-mediated gene silencing. However, knowledge about their functionality in rapeseed (Brassica napus) is rare. In the study, we identified and annotated 271 RNA helicase genes from B. napus using bioinformatics and high-throughput RNA-sequencing (RNA-seq). Three subfamilies DEAD-box, DEAH-box, or DExD/H-box have been identified. One hundred and ninety-five RNA helicases were confirmed by RNA-seq and 49 were identified to differentially respond to cadmium (Cd) stress (> 1...
April 2, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29606207/identification-of-mirnas-that-regulate-silique-development-in-brassica-napus
#7
Li Chen, Lei Chen, Xiangxiang Zhang, Tingting Liu, Sailun Niu, Jing Wen, Bin Yi, Chaozhi Ma, Jinxing Tu, Tingdong Fu, Jinxiong Shen
MicroRNAs (miRNAs) are a class of non-coding small RNAs (sRNAs) that play crucial regulatory roles in various developmental processes. Silique length indirectly influences seed yield in rapeseed (Brassica napus); however, the molecular roles of miRNAs in silique length are largely unknown. Here, backcross progenies of rapeseed with long siliques (LS) and short siliques (SS) were used to elucidate these roles. Four small RNA libraries from siliques in an early stage of development were sequenced, and a total of 814 non-redundant miRNA precursors were identified, representing 65 known and 394 novel miRNAs...
April 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29587414/identification-of-blueberry-mirnas-and-their-targets-based-on-high-throughput-sequencing-and-degradome-analyses
#8
Guangping Li, Yun Wang, Xiaoming Lou, Hailing Li, Changqing Zhang
miRNAs are important regulators of plant gene expression. To better characterize their functions, we applied high-throughput sequencing and degradome analyses to investigate three blueberry ( Vaccinium ashei ) tissues. A total of 127 known and 101 novel miRNAs were identified. Moreover, 141 targets for 42 known and 19 novel miRNAs were experimentally validated by degradome sequencing. A functional analysis of these miRNA targets revealed they were associated with diverse biological activities and several pathways, e...
March 26, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29515314/identification-and-comparative-analysis-of-micrornas-from-tomato-varieties-showing-contrasting-response-to-tolcv-infections
#9
Anita Tripathi, Kavita Goswami, Manish Tiwari, Sunil K Mukherjee, Neeti Sanan-Mishra
Increasing incidence of viral infections in crop plants adversely affects their growth and yield. Tomato ( Solanum lycopersicum ) is considered to be a favorite host for viruses with over 50 species of begomoviruses naturally infecting this crop. Tomato leaf curl virus (ToLCV) is among the most widespread and devastating begomoviruses affecting tomato production. microRNAs (miRs) have been established as key regulators of gene expression and plant development. The miR pathways are disturbed during infection by viruses...
March 2018: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/29507733/function-identification-of-mir482b-a-negative-regulator-during-tomato-resistance-to-phytophthora-infestans
#10
Ning Jiang, Jun Meng, Jun Cui, Guangxin Sun, Yushi Luan
Tomato is an important horticultural and economic crop cultivated worldwide. As Phytophthora infestans becomes a huge threat to tomato production, it is necessary to study the resistance mechanisms of tomato against P. infestans . Our previous research has found that miR482 might be involved in tomato- P . infestans interaction. In this study, miR482b precursor was cloned from Solanum pimpinellifolium "L3708" and miR482b was shown to decrease in abundance in tomato following P . infestans infection...
2018: Horticulture Research
https://www.readbyqxmd.com/read/29491170/expression-profiling-across-wild-and-cultivated-tomatoes-supports-the-relevance-of-early-mir482-2118-suppression-for-phytophthora-resistance
#11
Sophie de Vries, Andreas Kukuk, Janina K von Dahlen, Anika Schnake, Thorsten Kloesges, Laura E Rose
Plants possess a battery of specific pathogen resistance ( R- )genes. Precise R- gene regulation is important in the presence and absence of a pathogen. Recently, a microRNA family, miR482/2118, was shown to regulate the expression of a major class of R- genes , nucleotide-binding site leucine-rich repeats ( NBS-LRRs ). Furthermore, RNA silencing suppressor proteins, secreted by pathogens, prevent the accumulation of miR482/2118, leading to an upregulation of R- genes. Despite this transcriptional release of R -genes, RNA silencing suppressors positively contribute to the virulence of some pathogens ...
February 28, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29453091/identification-of-mirnas-and-their-targets-in-maize-in-response-to-sugarcane-mosaic-virus-infection
#12
Zihao Xia, Zhenxing Zhao, Mingjun Li, Ling Chen, Zhiyuan Jiao, Yuanhua Wu, Tao Zhou, Weichang Yu, Zaifeng Fan
MicroRNAs (miRNAs) are endogenous non-coding small RNAs that play essential regulatory roles in plant development and environmental stress responses. Maize (Zea mays L.) is a global economically important food and forage crop. To date, a number of maize miRNAs have been identified as being involved in plant development and stress responses. However, the miRNA-mediated gene regulatory networks responsive to virus infections in maize remain largely unknown. In this study, the profiles of small RNAs in buffer- and Sugarcane mosaic virus (SCMV)-inoculated maize plants were obtained by high-throughput sequencing, respectively...
April 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29440670/characterization-of-conserved-and-novel-micrornas-in-lilium-lancifolium-thunb-by-high-throughput-sequencing
#13
Xiangfeng He, Awraris Getachew Shenkute, Wenhe Wang, Shufa Xu
MicroRNAs (miRNAs) are among the class of noncoding small RNA molecules and play a crucial role in post-transcriptional regulation in plants. Although Lilium is one of the most popular ornamental flowers worldwide, however, there is no report on miRNAs identification. In the present study, therefore, miRNAs and their targets were identified from flower, leaf, bulblet and bulb of Lilium lancifolium Thunb. by high-throughput sequencing and bioinformatics analysis. In this study, a total of 38 conserved miRNAs belonging to 17 miRNA families and 44 novel miRNAs were identified...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29363419/phased-secondary-small-interfering-rnas-in-panaxnotoginseng
#14
Kun Chen, Li Liu, Xiaotuo Zhang, Yuanyuan Yuan, Shuchao Ren, Junqiang Guo, Qingyi Wang, Peiran Liao, Shipeng Li, Xiuming Cui, Yong-Fang Li, Yun Zheng
BACKGROUND: Recent results demonstrated that either non-coding or coding genes generate phased secondary small interfering RNAs (phasiRNAs) guided by specific miRNAs. Till now, there is no studies for phasiRNAs in Panax notoginseng (Burk.) F.H. Chen (P. notoginseng), an important traditional Chinese herbal medicinal plant species. METHODS: Here we performed a genome-wide discovery of phasiRNAs and its host PHAS loci in P. notoginseng by analyzing small RNA sequencing profiles...
January 19, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29362080/the-mirnaome-dynamics-during-developmental-and-metabolic-reprogramming-of-tomato-root-infected-with-potato-cyst-nematode
#15
Marek D Koter, Magdalena Święcicka, Mateusz Matuszkiewicz, Andrzej Pacak, Natalia Derebecka, Marcin Filipecki
Cyst-forming plant-parasitic nematodes are pests threatening many crops. By means of their secretions cyst nematodes induce the developmental and metabolic reprogramming of host cells that lead to the formation of a syncytium, which is the sole food source for growing nematodes. The in depth micro RNA (miRNA) dynamics in the syncytia induced by Globodera rostochiensis in tomato roots was studied. The miRNAomes were obtained from syncytia covering the early and intermediate developmental stages, and were the subject of differential expression analysis...
March 2018: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/29358038/comprehensive-analysis-of-mrna-internal-cleavage-sites-in-arabidopsis-thaliana
#16
Daishin Ueno, Shotaro Yamasaki, Taku Demura, Ko Kato
The major obstacle of efficient transgene expression seems to be gene silencing, and one of the important factors in gene silencing is mRNA stability. Regulation of mRNA stability is an important aspect of the control of gene expression. mRNAs are degraded by both exonucleolytic digestion and endonucleolytic cleavage. However, with the exception of small RNA-guided cleavage, the mechanisms underlying endonucleolytic cleavage-dependent RNA degradation remain to be elucidated. High-throughput approaches for genome-wide profiling of RNA cleavage sites, collectively termed degradome sequencing, have been developed by several groups...
January 18, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29318538/dissecting-degradomes-analysis-of-protease-coding-genes
#17
Ángel Álvarez-Eguiluz, Ander Díaz-Navarro, Xose S Puente
Proteases constitute up to 3% of all protein-coding genes in a vertebrate genome and participate in numerous physiological and pathological processes. The characterization of the degradome of one organism, the set of all genes encoding proteolytic enzymes, and the comparison to the degradome of other species have proved useful to identify genetic differences that are helpful to elucidate the molecular basis of diverse biological processes, the different susceptibility to disease, and the evolution of the structure and function of proteases...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29280990/dpmind-degradome-based-plant-mirna-target-interaction-and-network-database
#18
Yuhan Fei, Rui Wang, Haoyuan Li, Shu Liu, Hongsheng Zhang, Ji Huang
Motivation: MicroRNAs (miRNAs) play essential roles in plant growth, development and stress responses through post-transcriptionally regulating the expression levels of their target mRNAs. Although some tools and databases were developed for predicting the relationships between miRNAs and their targets (miR-Tar), most of them were dependent on computational methods without experimental validations. With development of degradome sequencing techniques, researchers can identify potential interactions based on degradome sequencing data...
December 21, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29236948/tarhunter-a-tool-for-predicting-conserved-microrna-targets-and-target-mimics-in-plants
#19
Xuan Ma, Chunyan Liu, Lianfeng Gu, Beixin Mo, Xiaofeng Cao, Xuemei Chen
Summary: In plants, the targets of deeply conserved microRNAs (miRNAs) were comprehensively studied. Evidence is emerging that targets of less conserved miRNAs, endogenous target mimics (eTM) and non-canonical targets play functional roles. Existing plant miRNA prediction tools lack a cross-species conservation filter and eTM prediction function. We developed a tool named TarHunter that features a strict cross-species conservation filter and capability of predicting eTMs. TarHunter has higher recall or precision rate as compared with other tools, and the conservation filter effectively increases prediction precision...
December 11, 2017: Bioinformatics
https://www.readbyqxmd.com/read/29236054/genome-wide-profiling-of-small-rnas-and-degradome-revealed-conserved-regulations-of-mirnas-on-auxin-responsive-genes-during-fruit-enlargement-in-peaches
#20
Mengya Shi, Xiao Hu, Yu Wei, Xu Hou, Xue Yuan, Jun Liu, Yueping Liu
Auxin has long been known as a critical phytohormone that regulates fruit development in plants. However, due to the lack of an enlarged ovary wall in the model plants Arabidopsis and rice, the molecular regulatory mechanisms of fruit division and enlargement remain unclear. In this study, we performed small RNA sequencing and degradome sequencing analyses to systematically explore post-transcriptional regulation in the mesocarp at the hard core stage following treatment of the peach ( Prunus persica L.) fruit with the synthetic auxin α-naphthylacetic acid (NAA)...
December 13, 2017: International Journal of Molecular Sciences
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