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Lu Hou, Yanhong Cui, Xiang Li, Wu Chen, Zhiyong Zhang, Xiaoming Pang, Yingyue Li
Thuja koraiensis Nakai is an endangered conifer of high economic and ecological value in Jilin Province, China. However, studies on its population structure and conservation genetics have been limited by the lack of genomic data. Here, 37,761 microsatellites (simple sequence repeat, SSR) were detected based on 875,792 de novo-assembled contigs using a restriction-associated DNA (RAD) approach. Among these SSRs, 300 were randomly selected to test for polymorphisms and 96 obtained loci were able to amplify a fragment of expected size...
April 17, 2018: Genes
Giorgia Maria Bosi, Andrew Cook, Rajan Rai, Leon J Menezes, Silvia Schievano, Ryo Torii, Gaetano Burriesci
During Atrial Fibrillation (AF) more than 90% of the left atrial thrombi responsible for thromboembolic events originate in the left atrial appendage (LAA), a complex small sac protruding from the left atrium (LA). Current available treatments to prevent thromboembolic events are oral anticoagulation, surgical LAA exclusion, or percutaneous LAA occlusion. However, the mechanism behind thrombus formation in the LAA is poorly understood. The aim of this work is to analyse the hemodynamic behaviour in four typical LAA morphologies - "Chicken wing", "Cactus", "Windsock" and "Cauliflower" - to identify potential relationships between the different shapes and the risk of thrombotic events...
2018: Frontiers in Cardiovascular Medicine
Firoz Hossain, Vignesh Muthusamy, Neha Pandey, Ashish K Vishwakarma, Aanchal Baveja, Rajkumar U Zunjare, Nepolean Thirunavukkarasu, Supradip Saha, Kanchikeri M Manjaiah Manjaiah, Boddupalli M Prasanna, Hari S Gupta
Maize is a valuable source of food and feed worldwide. Maize endosperm protein is, however nutritionally poor due to the reduced levels of two essential amino acids, lysine and tryptophan. In this study, recessive opaque2 (o2) allele that confers enhanced endosperm lysine and tryptophan, was introgressed using marker-assisted backcross breeding into three normal inbred lines (HKI323, HKI1105 and HKI1128). These are the parental lines of three popular medium-maturing single cross hybrids (HM4, HM8 and HM9) in India...
March 2018: Journal of Genetics
Soleimani Gezeljeh Ali, Reza Darvishzadeh, Asa Ebrahimi, Mohammad Reza Bihamta
Sunflower is an important source of edible oil. Drought is known as an important factor limiting the growth and productivity of field crops in most parts of the world. Agricultural biotechnology mainly aims at developing crops with higher tolerance to the challenging environmental conditions, such as drought. This study examined a number of morphological characters, along with relative water content (RWC) in 100 inbred sunflower lines. A 10 × 10 simple lattice design with two replications was employed to measure the mentioned parameters under natural and water-limited states during two successive years...
March 2018: Journal of Genetics
Ramesh K Singh, N Rai, Amareesh K Singh, Prashant Kumar, T Chaubey, B Singh, Satya N Singh
Genetic diversity during prebreeding or postbreeding programme, is the key pillar to characterize the valuable traits and gene of interest. Whereas, superior or inferior heterotic performance of F1 depend on the diverse nature of their pedigree. Therefore, the aim of this study was to see the diversity between the interspecific crosses and effect of heterosis, and inheritance for the morphological traits and ToLCV resistance. All the 24 F1 interspecific crosses were classified into four clusters on the basis of morphological traitsas well as simple sequence repeat (SSR) markers...
March 2018: Journal of Genetics
Seong W Kang, Srinivas Jayanthi, Gurueswar Nagarajan, Thallapuranam Krishnaswamy Suresh Kumar, Wayne J Kuenzel
Vasotocin 1a and 1b receptors (V1aR and V1bR) have been shown to play important roles in the neuroendocrine regulation of stress responses via the anterior pituitary (AP) of birds. To identify effective subtype-specific antagonists for the chicken V1aR (cV1aR) and cV1bR, potential antagonists to the mammalian V1R were screened against the cV1aR and cV1bR 3D structural models by molecular docking analysis with determination of binding pocket / amino acid residues involved in the interaction. The antagonistic effects of the selected ligands were examined by measuring pro-opiomelanocortin (POMC) heteronuclear RNA (hnPOMC) levels following the in vitro stress administration to primary chicken AP cells...
April 16, 2018: Journal of Biomolecular Structure & Dynamics
Franziska Kappert, Sridhar Sreeramulu, Hendrik R A Jonker, Christian Richter, Vladimir V Rogov, Ewgenij Proschak, Bruno Hargittay, Krishna Saxena, Harald Schwalbe
The interaction of fibroblast growth factors (FGFs) with their fibroblast growth factor receptors (FGFRs) are important in the signaling network of cell growth and development. SSR128129E (SSR)[1,2], a ligand of small molecular weight with potential anti-cancer properties, acts allosterically on the extracellular domains of FGFRs. Up to now, the structural basis of SSR binding to the D3 domain of FGFR remained elusive. Here, we report the structural characterization of the interaction of SSR with one specific receptor, FGFR3 by NMR spectroscopy...
April 14, 2018: Chemistry: a European Journal
Chuan-Jie Zhang, Min-Jung Yook, Hae-Rim Park, Soo-Hyun Lim, Jin-Won Kim, Jong-Seok Song, Gyoungju Nah, Hae-Ryong Song, Beom-Ho Jo, Kyung Hee Roh, Suhyoung Park, Young-Seok Jang, Ill-Sup Noua, Do-Soon Kim
Pollen-mediated gene flow (PMGF) from genetically modified (GM) Brassica napus to its wild relatives by wind and insects is a major ecological concern in agricultural ecosystems. This study conducted is to estimate maximum potential gene flow and differentiate between wind- and bee-mediated gene flows from herbicide resistant (HR) B. napus to its closely-related male sterile (MS) relatives, B. napus, B. juncea and Raphanus sativus. Various markers, including pods formation in MS plants, herbicide resistance, and SSR markers, were used to identify the hybrids...
April 10, 2018: Science of the Total Environment
Bing Feng, Soojin V Yi, Manman Zhang, Xiaoyun Zhou
The co-existence of several ploidy types in natural populations makes the cyprinid loach Misgurnus anguillicaudatus an exciting model system to study the genetic and phenotypic consequences of ploidy variations. A first step in such effort is to identify the specific ploidy of an individual. Currently popular methods of karyotyping via cytological preparation or flow cytometry require a large amount of tissue (such as blood) samples, which can be damaging or fatal to the fishes. Here, we developed novel microsatellite markers (SSR markers) from M...
2018: PloS One
Si-Qi Tao, Bin Cao, Cheng-Ming Tian, Ying-Mei Liang
The Apple-Juniper rust, Gymnosporangium yamadae , is an economically important pathogen of apples and junipers in Asia. The absence of markers has hampered the study of the genetic diversity of this widespread pathogen. In our study, we developed twenty-two novel microsatellite markers for G. yamadae from randomly sequenced regions of the transcriptome, using next-generation sequencing methods. These polymorphic markers were also tested on 96 G. yamadae individuals from two geographical populations. The allele numbers ranged from 2 to 9 with an average value of 6 per locus...
April 12, 2018: International Journal of Molecular Sciences
Zhuanrong Wang, Lili Wan, Qiang Xin, Ye Chen, Xiaohui Zhang, Faming Dong, Dengfeng Hong, Guangsheng Yang
Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is the most serious disease affecting the yield of the agriculturally and economically important crop, Brassica napus L. (rapeseed). In this study, Oryza sativa polygalacturonase-inhibiting protein 2 (OsPGIP2) effectively enhanced rapeseed immunity against S. sclerotiorum infection. Leaf extracts of B. napus plants overexpressing OsPGIP2 showed enhanced S. sclerotiorum resistance by delaying pathogen infection. The constitutive expression of OsPGIP2 in rapeseed plants provided a rapid and potentiated defense response, including the production of reactive oxygen species, interaction with S...
April 10, 2018: Journal of Experimental Botany
Ezio Portis, Sergio Lanteri, Lorenzo Barchi, Flavio Portis, Luisa Valente, Laura Toppino, Giuseppe L Rotino, Alberto Acquadro
We have characterized the simple sequence repeat (SSR) markers of the eggplant ( Solanum melongena ) using a recent high quality sequence of its whole genome. We found nearly 133,000 perfect SSRs, a density of 125.5 SSRs/Mbp, and also about 178,400 imperfect SSRs. Of the perfect SSRs, 15.6% were complex, with two stretches of repeats separated by an intervening block of <100 nt. Di- and trinucleotide SSRs accounted, respectively, for 43 and 37% of the total. The SSRs were classified according to their number of repeats and overall length, and were assigned to their linkage group...
2018: Frontiers in Plant Science
M Rosling, C Trenker, A Neesse, C Görg
Introduction: Both B-mode ultrasound and contrast-enhanced ultrasound (CEUS) are well established procedures when diagnosing traumatic splenic ruptures (TSR). To date, there are no data about CEUS patterns in spontaneous splenic ruptures (SSR). It remains unknown whether TSR and SSR differ with respect to clinical characteristics, B-mode and CEUS characteristics. Patients and Methods: Between 12/2003 and 2/2010, n=33 SSRs and n=29 TSRs were diagnosed in a tertiary referral center...
January 2018: Ultrasound International Open
Surojit Sen, Budheswar Dehury, Jagajjit Sahu, Sunayana Rathi, Raj Narain Singh Yadav
Euphorbiaceae represents flowering plants family of tropical and sub-tropical region rich in secondary metabolites of economic importance. To understand and assess the genetic makeup among the members, this study was undertaken to characterize and compare SSR markers from publicly available ESTs and GSSs of nine selected species of the family. Mining of SSRs was performed by MISA, primer designing by Primer3, while functional annotation, gene ontology (GO) and enrichment analysis were performed by Blast2GO...
April 6, 2018: Molecular Biology Reports
Muqing Yan, David H Byrne, Patricia E Klein, Jizhou Yang, Qianni Dong, Natalie Anderson
Roses, which have been cultivated for at least 5000 years, are one of the most important ornamental crops in the world. Because of the interspecific nature and high heterozygosity in commercial roses, the genetic resources available for rose are limited. To effectively identify markers associated with QTL controlling important traits, such as disease resistance, abundant markers along the genome and careful phenotyping are required. Utilizing genotyping by sequencing technology and the strawberry genome ( Fragaria vesca v2...
2018: Horticulture Research
Kittipat Ukoskit, Ganlayarat Posudsavang, Nattapat Pongsiripat, Prasert Chatwachirawong, Peeraya Klomsa-Ard, Patthinun Poomipant, Somvong Tragoonrung
Sugar-related traits are of great importance in sugarcane breeding. In the present study, quantitative trait loci (QTL) mapping validated with association mapping was used to identify expressed sequence tag-simple sequence repeats (EST-SSRs) associated with sugar-related traits. For linkage mapping, 524 EST-SSRs, 241 Amplified Fragment Length Polymorphisms, and 10 genomic SSR markers were mapped using 283 F1 progenies derived from an interspecific cross. Six regions were identified using Multiple QTL Mapping, and 14 unlinked markers using single marker analysis...
March 31, 2018: Genomics
Andrew C Lewin, Aaron W Kolb, Gillian J McLellan, Ellison Bentley, Kristen A Bernard, Sandra P Newbury, Curtis R Brandt
Feline herpes virus type 1 (FHV-1) is widely considered to be the leading cause of ocular disease in cats and has been implicated in upper respiratory tract infections. Little, however is known about interstrain phylogenetic relationships, and details of the genomic structure. For the present study, twenty-six FHV-1 isolates from different cats in animal shelters were collected from eight separate locations in the USA, and the genomes sequenced. Genomic characterization of these isolates includied short sequence repeat (SSR) detection, with fewer SSRs detected, compared to herpes simplex viruses type 1 and 2...
March 29, 2018: Virology
Tianheng Ren, Yangshan Hu, Yingzi Tang, Chunsheng Li, Benju Yan, Zhenglong Ren, Feiquan Tan, Zongxiang Tang, Shulan Fu, Zhi Li
Maximum tiller number and productive tiller number are important traits for wheat grain yield, but research involving the temporal expression of tiller number at different quantitative trait loci (QTL) levels is limited. In the present study, a population set of 371 recombined inbred lines derived from a cross between Chuan-Nong18 and T1208 was used to construct a high-density genetic map using a Wheat55K SNP Array and to perform dynamic QTL analysis of the tiller number at four growth stages. A high-density genetic map containing 11,583 SNP markers and 59 SSR markers that spanned 4,513...
2018: Frontiers in Plant Science
Yanru Cui, Wenying Zhang, Xiuyun Lin, Shizhong Xu, Jianlong Xu, Zhikang Li
Drought is the most important factor limiting rice yield in most rainfed areas of Asia and Africa. Four large BC2 F2 populations consisted of 3,200 individuals, which were derived from crosses between an elite Geng variety, Jigeng88, and four donors from three different countries, were screened and progeny tested under severe drought stress, resulting in the development of 72 introgression lines (ILs) with significantly improved yield compared to the recurrent parent Jigeng88. These DT ILs plus four random populations (without drought selection population) from the same crosses were evaluated in replicated trials under both drought stress and non-stress conditions in two environments, and characterized with simple sequence repeat (SSR) markers to understand how directional selection was operating on the genetic variation of DT of rice...
2018: Frontiers in Plant Science
Dong Tang, Shouli Feng, Sai Li, Yu Chen, Baoliang Zhou
Gossypium bickii: (2n = 26, G1 G1 ), a wild diploid cotton, carries many favourable traits. However, these favourable traits cannot be directly transferred into G. hirsutum (2n = 52, AADD) cultivars due to the differences in genomes. Monosomic alien addition lines (MAALs) are considered an invaluable tool for the introgression of genes of interest from wild relatives into cultivated crops. In this study, the G. hirsutum-G. bickii amphidiploid (2n = 78, AADDG1 G1 ) was backcrossed with G. hirsutum to develop alien additions containing individual G...
March 27, 2018: Molecular Genetics and Genomics: MGG
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