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https://www.readbyqxmd.com/read/28550408/bacillus-pumilus-strain-yspmk11-as-plant-growth-promoter-and-bicontrol-agent-against-sclerotinia-sclerotiorum
#1
Manoj Kaushal, Ajay Kumar, Rajesh Kaushal
A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35 °C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus...
June 2017: 3 Biotech
https://www.readbyqxmd.com/read/28549456/association-mapping-of-loci-controlling-genetic-and-environmental-interaction-of-soybean-flowering-time-under-various-photo-thermal-conditions
#2
Tingting Mao, Jinyu Li, Zixiang Wen, Tingting Wu, Cunxiang Wu, Shi Sun, Bingjun Jiang, Wensheng Hou, Wenbin Li, Qijian Song, Dechun Wang, Tianfu Han
BACKGROUND: Soybean (Glycine max (L.) Merr.) is a short day plant. Its flowering and maturity time are controlled by genetic and environmental factors, as well the interaction between the two factors. Previous studies have shown that both genetic and environmental factors, mainly photoperiod and temperature, control flowering time of soybean. Additionally, these studies have reported gene × gene and gene × environment interactions on flowering time. However, the effects of quantitative trait loci (QTL) in response to photoperiod and temperature have not been well evaluated...
May 26, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28549206/assessment-of-allelic-diversity-among-drought-resistant-cotton-genotypes-using-microsatellite-markers
#3
A Javaid, F S Awan, F M Azhar, I A Khan
Drought, in conjunction with high temperature, is an important environmental constraint to cotton production. Development of cotton varieties with increased tolerance against adverse environmental conditions has been proposed as effective strategy for ensuring reliable yields. In the present study, 30 simple sequence repeat (SSR) primers were used to estimate genetic divergence among 22 cotton genotypes for drought stress tolerance. Genetic diversity is a prerequisite for developing drought resistant cotton genotypes...
May 25, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28546386/assessment-of-gene-flow-between-gossypium-hirsutum-and-gossypium-herbaceum-evidence-of-unreduced-gametes-in-the-diploid-progenitor
#4
E Montes, O Coriton, F Eber, V Huteau, J M Lacape, C Reinhardt, D Marais, J L Hofs, A M Chèvre, C Pannetier
In the framework of a gene flow assessment, we investigated the natural hybridization rate between Gossypium hirsutum (AADD genome) and G. herbaceum (AA genome). The latter species, a diploid progenitor of G. hirsutum, is spontaneously present in South Africa. Reciprocal crosses were performed without emasculation between G. herbaceum and G. hirsutum Neither examination of the morphological characteristics nor flow cytometry analysis of the 335 plants resulting from the G. hirsutum x G. herbaceum cross showed any hybrid features...
May 25, 2017: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/28545396/transcriptomic-resources-for-the-medicinal-legume-mucuna-pruriens-de-novo-transcriptome-assembly-annotation-identification-and-validation-of-est-ssr-markers
#5
N Sathyanarayana, Ranjith Kumar Pittala, Pankaj Kumar Tripathi, Ratan Chopra, Heikham Russiachand Singh, Vikas Belamkar, Pardeep Kumar Bhardwaj, Jeff J Doyle, Ashley N Egan
BACKGROUND: The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson's drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M...
May 25, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28542459/identification-and-evaluation-of-resistance-to-powdery-mildew-and-yellow-rust-in-a-wheat-mapping-population
#6
Lijun Yang, Xuejiang Zhang, Xu Zhang, Jirui Wang, Mingcheng Luo, Mujun Yang, Hua Wang, Libo Xiang, Fansong Zeng, Dazhao Yu, Daolin Fu, Garry M Rosewarne
Deployment of cultivars with genetic resistance is an effective approach to control the diseases of powdery mildew (PM) and yellow rust (YR). Chinese wheat cultivar XK0106 exhibits high levels of resistance to both diseases, while cultivar E07901 has partial, adult plant resistance (APR). The aim of this study was to map resistance loci derived from the two cultivars and analyze their effects against PM and YR in a range of environments. A doubled haploid population (388 lines) was used to develop a framework map consisting of 117 SSR markers, while a much higher density map using the 90K Illumina iSelect SNP array was produced with a subset of 80 randomly selected lines...
2017: PloS One
https://www.readbyqxmd.com/read/28542457/genet-specific-dna-methylation-probabilities-detected-in-a-spatial-epigenetic-analysis-of-a-clonal-plant-population
#7
Kiwako S Araki, Takuya Kubo, Hiroshi Kudoh
In sessile organisms such as plants, spatial genetic structures of populations show long-lasting patterns. These structures have been analyzed across diverse taxa to understand the processes that determine the genetic makeup of organismal populations. For many sessile organisms that mainly propagate via clonal spread, epigenetic status can vary between clonal individuals in the absence of genetic changes. However, fewer previous studies have explored the epigenetic properties in comparison to the genetic properties of natural plant populations...
2017: PloS One
https://www.readbyqxmd.com/read/28542267/discerning-morpho-anatomical-physiological-and-molecular-multiformity-in-cultivated-and-wild-genotypes-of-lentil-with-reconciliation-to-salinity-stress
#8
Dharmendra Singh, Chandan Kumar Singh, Shanti Kumari, Ram Sewak Singh Tomar, Sourabh Karwa, Rajendra Singh, Raja Bahadur Singh, Susheel Kumar Sarkar, Madan Pal
One hundred and sixty two genotypes of different Lens species were screened for salinity tolerance in hydroponics at 40, 80 and 120 mM sodium chloride (NaCl) for 30 d. The germination, seedling growth, biomass accumulation, seedling survivability, salinity scores, root and shoot anatomy, sodium ion (Na+), chloride ion (Cl-) and potassium ion (K+) concentrations, proline and antioxidant activities were measured to evaluate the performance of all the genotypes. The results were compared in respect of physiological (Na+, K+ and Cl-) and seed yield components obtained from field trials for salinity stress conducted during two years...
2017: PloS One
https://www.readbyqxmd.com/read/28539931/characterization-and-phylogenetic-analysis-of-ancient-italian-landraces-of-pear
#9
Nicoletta Ferradini, Hovirag Lancioni, Renzo Torricelli, Luigi Russi, Isabella Dalla Ragione, Irene Cardinali, Gianpiero Marconi, Mauro Gramaccia, Luciano Concezzi, Alessandro Achilli, Fabio Veronesi, Emidio Albertini
Pear is one of the oldest fruit tree crops and the third most important temperate fruit species. Its domestication took place independently in the Far East (China) and in the Caucasus region. While the origin of Eastern Asian cultivars is clear, that of European cultivars is still in doubt. Italy has a wealth of local varieties and genetic resources safeguarded by several public and private collections to face the erosion caused by the introduction of improved varieties in specialized orchards. The objectives of the present study were: (i) to characterize the existing germplasm through nuclear (SSR) and (ii) to clarify the genetic divergence between local and cultivated populations through chloroplast DNA (cpDNA) markers in order to provide insights into phylogenetic relationships of Pyrus spp...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28539617/characterization-of-porcine-simple-sequence-repeat-variation-on-a-population-scale-with-genome-resequencing-data
#10
Congcong Liu, Yan Liu, Xinyi Zhang, Xuewen Xu, Shuhong Zhao
Simple sequence repeats (SSRs) are used as polymorphic molecular markers in many species. They contribute very important functional variations in a range of complex traits; however, little is known about the variation of most SSRs in pig populations. Here, using genome resequencing data, we identified ~0.63 million polymorphic SSR loci from more than 100 individuals. Through intensive analysis of this dataset, we found that the SSR motif composition, motif length, total length of alleles and distribution of alleles all contribute to SSR variability...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28539606/sesamefg-an-integrated-database-for-the-functional-genomics-of-sesame
#11
Xin Wei, Hao Gong, Jingyin Yu, Pan Liu, Linhai Wang, Yanxin Zhang, Xiurong Zhang
Sesame (Sesamum indicum L.) has high oil content, a small diploid genome and a short growth period, making it an attractive species for genetic studies on oilseed crops. With the advancement of next-generation sequencing technology, genomics and functional genomics research of sesame has developed quickly in the last few years, and large amounts of data have been generated. However, these results are distributed in many different publications, and there is a lack of integration. To promote functional genomics research of sesame, we collected genetic information combined with comprehensive phenotypic information and integrated them in the web-based database named SesameFG...
May 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28529834/development-and-characterization-of-est-ssr-markers-for-begonia-luzhaiensis-begoniaceae
#12
Yu-Hsin Tseng, Han-Yau Huang, Wei-Bin Xu, Hsun-An Yang, Yan Liu, Ching-I Peng, Kuo-Fang Chung
PREMISE OF THE STUDY: Microsatellite primers were developed for Begonia luzhaiensis (Begoniaceae) to assess genetic diversity and population genetic structure. METHODS AND RESULTS: Based on the transcriptome data of B. luzhaiensis, 60 primer pairs were selected for initial validation, of which 16 yielded polymorphic microsatellite loci in 57 individuals. The number of alleles observed for these 16 loci ranged from one to nine. The observed and expected heterozygosity ranged from 0...
May 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28529536/mid-infrared-spectroscopy-combined-with-chemometrics-to-detect-sclerotinia-stem-rot-on-oilseed-rape-brassica-napus-l-leaves
#13
Chu Zhang, Xuping Feng, Jian Wang, Fei Liu, Yong He, Weijun Zhou
BACKGROUND: Detection of plant diseases in a fast and simple way is crucial for timely disease control. Conventionally, plant diseases are accurately identified by DNA, RNA or serology based methods which are time consuming, complex and expensive. Mid-infrared spectroscopy is a promising technique that simplifies the detection procedure for the disease. Mid-infrared spectroscopy was used to identify the spectral differences between healthy and infected oilseed rape leaves. Two different sample sets from two experiments were used to explore and validate the feasibility of using mid-infrared spectroscopy in detecting Sclerotinia stem rot (SSR) on oilseed rape leaves...
2017: Plant Methods
https://www.readbyqxmd.com/read/28528745/measurement-of-electrochemical-conductance-of-penile-skin-using-sudoscan-%C3%A2-a-new-tool-to-assess-neurogenic-impotence
#14
Jean-Pascal Lefaucheur
OBJECTIVES: To investigate the value of electrochemical skin conductance (ESC) measurement at penile level using Sudoscan(®) for the diagnosis of neurogenic impotence in diabetics. METHODS: The following neurophysiological parameters were assessed in 25 male diabetics who complained of impotence and 25 age-matched normal male subjects without erectile dysfunction (age range: 29-70 years): ESC, sympathetic skin responses (SSR), warm detection thresholds (WDT), and cold detection thresholds (CDT) for the penis and the feet, vibration detection thresholds (VDT) for the penis, and sensory nerve conduction study of the dorsal nerve of the penis (DNP) with sensory nerve action potential (SNAP) recording...
May 18, 2017: Neurophysiologie Clinique, Clinical Neurophysiology
https://www.readbyqxmd.com/read/28526937/molecular-confirmation-of-intraspecific-tomato-solanum-lycopersicum-hybrids-and-their-evaluation-against-late-blight-and-cucumber-mosaic-virus
#15
Amjad Hameed, Muhammad Yussouf Saleem, Khalid Pervaiz Akhtar, Muhammad Shoaib, Qumer Iqbal, Muhammad Asghar
Tomato is one of the most consumed vegetables in the world. Diseases are the number one concern in the development of high-yield and disease-resistant tomato hybrids which is the foremost priority of breeders. Present study was conducted (1) to develop DNA-based markers for genetic confirmation of tomato F1 hybrids, (2) to utilize sequenced characterized amplified region (SCAR) marker linked to the Ph-3 gene for Phytophthora infestans resistance in tomato and (3) to evaluate male and female parental genotypes and their F1 hybrids against late blight (LB) and cucumber mosaic virus (CMV)...
May 19, 2017: Molecular Biotechnology
https://www.readbyqxmd.com/read/28523007/transcriptomic-identification-of-drought-related-genes-and-ssr-markers-in-sudan-grass-based-on-rna-seq
#16
Yongqun Zhu, Xia Wang, Linkai Huang, Chaowen Lin, Xinquan Zhang, Wenzhi Xu, Jianhua Peng, Zhou Li, Haidong Yan, Fuxiang Luo, Xie Wang, Li Yao, Dandan Peng
Sudan grass (Sorghum sudanense) is an annual warm-season gramineous forage grass that is widely used as pasture, hay, and silage. However, drought stress severely impacts its yield, and there is limited information about the mechanisms of drought tolerance in Sudan grass. In this study, we used next-generation sequencing to identify differentially expressed genes (DEGs) in the Sudan grass variety Wulate No.1, and we developed simple sequence repeat (SSR) markers associated with drought stress. From 852,543,826 raw reads, nearly 816,854,366 clean reads were identified and used for analysis...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28513283/molecular-characterisation-of-australian-isolates-of-puccinia-graminis-f-sp-tritici-supports-long-term-clonality-but-also-reveals-cryptic-genetic-variation
#17
Jianping Zhang, Peng Zhang, Haydar Karaoglu, Robert F Park
Long term surveys of pathogenicity in Puccinia graminis f. sp. tritici (Pgt) in Australia have implicated mutation as a major source of virulence, at times leading to the demise of stem rust resistant wheat cultivars and substantial yield losses. Since 1925, these surveys have identified at least four occasions on which exotic isolates of Pgt appeared in Australia, with each acting as a founding isolate that gave rise sequentially to derivative pathotypes via presumed single step mutation. The current study aimed to examine the relationship between virulence and molecular patterns using SSR markers on selected isolates of Pgt collected in Australia during a 52 year period in order to propose an evolutionary pathway...
May 17, 2017: Phytopathology
https://www.readbyqxmd.com/read/28512468/molecular-identification-and-karyological-analysis-of-a-rampant-aspen-populus-tremula-l-salicaceae-clone
#18
Dmitry V Politov, Yuri S Belokon, Anna V Shatokhina, Maryana M Belokon, Nail A Khanov, Elena A Mudrik, Tatyana A Polyakova, Anna B Azarova, Konstantin A Shestibratov
A rampant highly heterozygous aspen (Populus tremula L.) clone "Meshabash" has been revealed in course of population genetic diversity analysis in a native stand in the Republic of Tatarstan, Russia. Here we report the results of karyological analysis showing that this highly vigorous clone is diploid (2n = 38) while typically triploid aspen demonstrates increased growth rate and resistance to aspen trunk rot caused by fungus Phellinus tremulae. By means of DNA identification of a series of model trees using 14 SSR loci we outlined the area occupied by this clone (at least 1...
2017: International Journal of Plant Genomics
https://www.readbyqxmd.com/read/28510247/characterization-and-development-of-56-est-ssr-markers-derived-from-the-transcriptome-of-odontobutis-potamophila
#19
P P Wang, M Ren, S Q Chen, S W Yin, C Zhao, H Y Zhang, X R Li, Q Q Cao, G Q Zhou
Expressed sequence tags (ETSs) are the sources of microsatellite development. In this study, we isolated and characterized microsatellite markers for Odontobutis potamophila by using Illumina RNA-sequencing. We sequenced a large number of ESTs and screened 200 potential microsatellites. Consequently, a total of 56 novel polymorphic microsatellite repeat markers were identified in thirty-two individuals from a wild population area (Jiande, Zhejiang Province, China). The number of alleles per locus varied from two to eight, the observed heterozygosity (HO) ranged from 0...
May 10, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28508097/development-of-ssr-markers-and-identification-of-major-quantitative-trait-loci-controlling-shelling-percentage-in-cultivated-peanut-arachis-hypogaea-l
#20
Huaiyong Luo, Zhijun Xu, Zhendong Li, Xinping Li, Jianwei Lv, Xiaoping Ren, Li Huang, Xiaojing Zhou, Yuning Chen, Jingyin Yu, Weigang Chen, Yong Lei, Boshou Liao, Huifang Jiang
A total of 204,439 SSR markers were developed in diploid genomes, and 25 QTLs for shelling percentage were identified in a RIL population across 4 years including five consistent QTLs. Cultivated peanut (Arachis hypogaea L.) is an important grain legume providing edible oil and protein for human nutrition. Genome sequences of its diploid ancestors, Arachis duranensis and A. ipaensis, were reported, but their SSRs have not been well exploited and utilized hitherto. Shelling percentage is an important economic trait and its improvement has been one of the major objectives in peanut breeding programs...
May 15, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
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