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https://www.readbyqxmd.com/read/29922302/global-qtl-analysis-identifies-genomic-regions-on-chromosomes-4a-and-4b-harboring-stable-loci-for-yield-related-traits-across-different-environments-in-wheat-triticum-aestivum-l
#1
Panfeng Guan, Lahu Lu, Lijia Jia, Muhammad Rezaul Kabir, Jinbo Zhang, Tianyu Lan, Yue Zhao, Mingming Xin, Zhaorong Hu, Yingyin Yao, Zhongfu Ni, Qixin Sun, Huiru Peng
Major advances in wheat production are needed to address global food insecurity under future climate conditions, such as high temperatures. The grain yield of bread wheat ( Triticum aestivum L.) is a quantitatively inherited complex trait that is strongly influenced by interacting genetic and environmental factors. Here, we conducted global QTL analysis for five yield-related traits, including spike yield, yield components and plant height (PH), in the Nongda3338/Jingdong6 doubled haploid (DH) population using a high-density SNP and SSR-based genetic map...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29922259/genetic-diversity-studies-based-on-morphological-variability-pathogenicity-and-molecular-phylogeny-of-the-sclerotinia-sclerotiorum-population-from-indian-mustard-brassica-juncea
#2
Pankaj Sharma, Amos Samkumar, Mahesh Rao, Vijay V Singh, Lakshman Prasad, Dwijesh C Mishra, Ramcharan Bhattacharya, Navin C Gupta
White mold or stem rot disease are ubiquitously distributed throughout the world and the causal organism of this disease Sclerotinia sclerotiorum (Lib.) de Bary, is known to infect over 400 plant species. Sclerotinia stem rot is one of the most devastating fungal diseases and poses a serious threat to the worldwide cultivation of oilseed Brassica including India. S. sclerotiorum pathogen usually infects the stem but in severe cases leaves and pods also affected at different developmental stages that deteriorate not only the oil quality but also causing the seed and oil yield losses up to 90% depending on the severity of the disease infestation...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29916885/direct-to-implant-as-a-frontline-option-for-immediate-breast-reconstruction-a-comparative-study-with-2-stage-reconstruction
#3
Hyun Ho Han, Hyung Bae Kim, Eun Key Kim, Jin Sup Eom
BACKGROUND: Immediate single-stage direct-to-implant breast reconstruction requires caution owing to the possibility of skin necrosis and implant failure. Nevertheless, this method has been performed widely for breast reconstruction. This study aimed to analyze the safety of single-stage implant reconstruction by comparing it with 2-stage reconstruction (TSR). METHODS: Immediate single-stage reconstructions (SSRs) and TSRs with a tissue expander, performed from January 2011 to December 2016, were retrospectively reviewed...
June 19, 2018: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/29914361/genome-wide-characterization-of-simple-sequence-repeats-in-pyrus-bretschneideri-and-their-application-in-an-analysis-of-genetic-diversity-in-pear
#4
Huabai Xue, Pujuan Zhang, Ting Shi, Jian Yang, Long Wang, Suke Wang, Yanli Su, Huirong Zhang, Yushan Qiao, Xiugen Li
BACKGROUND: Pear (Pyrus spp.) is an economically important temperate fruit tree worldwide. In the past decade, significant progress has been made in pear molecular genetics based on DNA research, but the number of molecular markers is still quite limited, which hardly satisfies the increasing needs of geneticists and breeders. RESULTS: In this study, a total of 156,396 simple sequence repeat (SSR) loci were identified from a genome sequence of Pyrus bretschneideri 'Dangshansuli'...
June 18, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29909526/a-bean-common-mosaic-virus-bcmv-resistance-gene-is-fine-mapped-to-the-same-region-as-rsv1-h-in-the-soybean-cultivar-suweon-97
#5
Mian Wu, Wen-Ping Wu, Cheng-Chen Liu, Ying-Na Liu, Xiao-Yi Wu, Fang-Fang Ma, An-Qi Zhu, Jia-Yin Yang, Bin Wang, Jian-Qun Chen
In the soybean cultivar Suweon 97, BCMV-resistance gene was fine-mapped to a 58.1-kb region co-localizing with the Soybean mosaic virus (SMV)-resistance gene, Rsv1-h raising a possibility that the same gene is utilized against both viral pathogens. Certain soybean cultivars exhibit resistance against soybean mosaic virus (SMV) or bean common mosaic virus (BCMV). Although several SMV-resistance loci have been reported, the understanding of the mechanism underlying BCMV resistance in soybean is limited. Here, by crossing a resistant cultivar Suweon 97 with a susceptible cultivar Williams 82 and inoculating 220 F2 individuals with a BCMV strain (HZZB011), we observed a 3:1 (resistant/susceptible) segregation ratio, suggesting that Suweon 97 possesses a single dominant resistance gene against BCMV...
June 16, 2018: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29907833/identification-of-rice-landraces-with-promising-yield-and-the-associated-genomic-regions-under-low-nitrogen
#6
I Subhakara Rao, C N Neeraja, B Srikanth, D Subrahmanyam, K N Swamy, K Rajesh, P Vijayalakshmi, T Vishnu Kiran, N Sailaja, P Revathi, P Raghuveer Rao, L V Subba Rao, K Surekha, V Ravindra Babu, S R Voleti
With the priority of the low input sustainable rice cultivation for environment friendly agriculture, NUE of rice becomes the need of the hour. A set of 472 rice genotypes comprising landraces and breeding lines were evaluated for two seasons under field conditions with low and recommended nitrogen and >100 landraces were identified with relative higher yield under low nitrogen. Donors were identified for higher N uptake, N translocation into grains and grain yield under low N. Grains on secondary branches, N content in grain and yield appears to be the selection criterion under low N...
June 15, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29907083/genomic-and-transcriptomic-sequencing-of-rosa-hybrida-provides-microsatellite-markers-for-breeding-flower-trait-improvement-and-taxonomy-studies
#7
Weicong Qi, Xi Chen, Peihong Fang, Shaochuan Shi, Jingjing Li, Xintong Liu, Xiaoqian Cao, Na Zhao, Huiyuan Hao, Yajie Li, Yujie Han, Zhao Zhang
BACKGROUND: Rosa hybrida is a valuable ornamental, food and medicinal crop worldwide, but with relatively limited molecular marker resources, especially for flower-specific markers. In this study, we performed genomic and floral transcriptomic sequencing of modern rose. We obtained comprehensive nucleotide information, from which numerous potential simple sequence repeat (SSR) markers were identified but were found to have high rates of amplification failure and PCR product redundancy...
June 15, 2018: BMC Plant Biology
https://www.readbyqxmd.com/read/29902971/genetic-dissection-and-fine-mapping-of-a-novel-dt-gene-associated-with-determinate-growth-habit-in-sesame
#8
Yanxin Zhang, Linhai Wang, Yuan Gao, Donghua Li, Jingyin Yu, Rong Zhou, Xiurong Zhang
BACKGROUND: As an important oil crop, growth habit of sesame (Sesamum indicum L.) is naturally indeterminate, which brings about asynchronous maturity of capsules and causes loss of yield. RESULTS: The genetic basis of determinate growth habit in sesame was investigated by classical genetic analysis through multiple populations, results revealed that it was controlled by an unique recessive gene. The genotyping by sequencing (GBS) approach was employed for high-throughput SNP identification and genotyping in the F2 population, then a high density bin map was constructed, the map was 1086...
June 14, 2018: BMC Genetics
https://www.readbyqxmd.com/read/29902894/-analysis-on-ssr-information-in-transcriptome-of-astragalus-membranaceus-var-mongholicus-and-its-polymorphism
#9
Run-Li He, Yi-Li Han, Fang Wang, Ji-Quan Liu, Li-Hua Wang
In order to enrich the library of SSR and provide more powerful tools for molecular marker-assisted breeding in Astragalus membranaceus var. mongholicus, simple sequence repeats (SSR) loci in its transcriptome were searched in 18 040 unigenes (>=1 kb) by using MISA. SSR loci information was analyzed and SSR primers were designed by Primer 3. Furthermore, 110 pairs of primers were randomly selected for the polymorphic analysis on 20 plants collected from different habitats. A total of 5 640 SSRs were found in the transcriptome of A...
May 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29902892/-establishment-of-dna-identity-card-and-analysis-of-genetic-similarity-among-58-varieties-in-lonicera-japonica
#10
Feng-Jie Zhu, Shan-Shan Zhang, Yuan Yuan, Jun-Hui Zhou, Chao Jiang, Jian Yang, Juan Liu, Nian-Jun Yu, Lu-Qi Huang
A total of 58 varieties in Lonicera japonica from 20 producing areas were amplified by 22 pairs of SSR primers. Seven pairs of polymorphic primers were screened and their primers were used to establish DNA identity card and analyze genetic similarity.All the 58 varieties could be distinguished each other by the DNA identity card constituted by 7 pairs of core SSR primers.The genetic similarity coefficients of 58 varieties ranged from 0.366 7 to 0.916 7 by using PopGene32(vesion1.32). Furthermore, all the varieties consistency were classified into 4 groups and constructed an evaluation table according to cluster analysis by an un-weighted pair-group average method with arithmetic mean...
May 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29892941/development-of-genic-ssr-markers-and-genetic-diversity-of-indian-lettuce-lactuca-indica-l-in-south-korea
#11
Bal Kumari Oliya, Moon Young Kim, Suk-Ha Lee
Indian lettuce (Lactuca indica L.) is an undomesticated wild plant with high economic potential. We know little about the plant's genome, such as its DNA markers, making genetic research using this plant difficult. In this study, 100 genic simple sequence repeat (SSR) primers with a 99-250 bp target amplicon were synthesized from L. indica transcriptomic sequences. These primers were examined in 8 diverse L. indica accessions, and 90 polymorphic SSRs were obtained. Twenty-three of the 90 polymorphic SSRs were used to investigate transferability to another two Lactuca species, Lactuca serriola and Lactuca sativa...
June 2018: Genes & Genomics
https://www.readbyqxmd.com/read/29892927/identification-of-novel-est-ssr-markers-by-transcriptome-sequencing-in-ridgetail-white-prawn-exopalaemon-carinicauda
#12
Jiajia Wang, Qianqian Ge, Jitao Li, Haiyu Gao, Jian Li, Fazhen Zhao
The ridgetail white prawn Exopalaemon carinicauda is one of the major commercial mariculture species in eastern China. However, only limited molecular markers are currently available due to the lack of genome information, which hinders its genetic and breeding studies. In this study, we identified new simple sequence repeats from transcriptome sequences by Illumina Hiseq 2500 platform. A total of 14273 SSR loci were identified from 130,082 assembled transcripts, with 6590 pairs of PCR primers designed. A total of 12,155 sequences containing SSR were predicted; and 2764 (22...
February 2018: Genes & Genomics
https://www.readbyqxmd.com/read/29882115/mating-patterns-and-pollen-dispersal-in-a-japanese-larch-larix-kaempferi-clonal-seed-orchard-a-case-study
#13
Xingbin Chen, Xiaomei Sun, Leiming Dong, Shougong Zhang
Pollination dynamics highly determines the genetic quality of seed orchard crops. However, there is less research about the effect of mating patterns on seed productivity of orchard crops. So far, clonal seed orchards have been producing genetically improved seedlings used for most Japanese larch (Larix kaempferi (Lamb.) Carr.) plantations in China. In the present study, a total of 17 highly variable simple sequence repeat (SSR) markers were used for genotyping a progeny trial population consisting of 647 open-pollinated progenies germinated from seeds which were collected from 63 maternal clones with 140 potential paternal clones in a Japanese larch clonal seed orchard in China...
June 4, 2018: Science China. Life Sciences
https://www.readbyqxmd.com/read/29879559/mutagenic-effects-of-spent-potliner-and-derivatives-on-allium-cepa-l-and-lactuca-sativa-l-a-molecular-approach
#14
Larissa Fonseca Andrade-Vieira, Paula Mauri Bernardes, Marcia Flores da Silva Ferreira
Spent potliner (SPL) is a solid residue generated by the aluminum industry. Its composition is variable and complex, containing fluoride and cyanide salts as well as aluminum, which contributes to its toxicity. SPL is sometimes released directly into the soil, where it is prone to leaching and has the potential to cause alterations and damage to DNA. Considering that polymorphism analysis of simple sequence repeat (SSR) and inter-simple sequence repeat (ISSR) DNA markers is an interesting tool to determine the mutagenicity of an environmental pollutant, the present study adopted this approach to verify the mutagenic potential of SPL and its main toxic components (aluminum, fluoride, and cyanide) on root tip cells of Lactuca sativa and Allium cepa...
May 30, 2018: Chemosphere
https://www.readbyqxmd.com/read/29876718/identification-of-charcoal-rot-resistance-qtls-in-sorghum-using-association-and-in-silico-analyses
#15
Amer F Mahmoud, Salah Fatouh Abou-Elwafa, Tariq Shehzad
Charcoal rot disease, a root and stem disease caused by the soil-borne fungus Macrophomina phaseolina (Tassi) Goid., is a major biotic stress that limits sorghum productivity worldwide. Charcoal rot resistance-related parameters, e.g., pre-emergence damping-off%, post-emergence damping-off%, charcoal rot disease severity, and plant survival rates, were measured in a structured sorghum population consisting of 107 landraces. Analysis of variance of charcoal rot resistance-related parameters revealed significant variations in the response to M...
June 7, 2018: Journal of Applied Genetics
https://www.readbyqxmd.com/read/29876074/development-of-high-transferability-cpssr-markers-for-individual-identification-and-genetic-investigation-in-cupressaceae-species
#16
Li-Sha Huang, Yan-Qiang Sun, Yuqing Jin, Qiong Gao, Xian-Ge Hu, Fu-Ling Gao, Xiao-Lei Yang, Ji-Jun Zhu, Yousry A El-Kassaby, Jian-Feng Mao
Given the low substitution rate in plastomes, the polymorphic and codominant nature of chloroplast SSRs (cpSSRs) makes them ideal markers, complementing their nuclear counterpart. In Cupressaceae, cpSSRs are mostly paternally inherited, thus, they are useful in mating systems and pollen flow studies. Using e-PCR, 92 SSR loci were identified across six Cupressaceae plastomes, and primers were designed for 26 loci with potential interspecific transferability. The 26 developed cpSSRs were polymorphic in four genera, Platycladus , Sabina , Juniperus, and Cupressus and are suitable for Cupressaceae molecular genetic studies and utilization...
May 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29875610/screening-of-clubroot-resistant-varieties-and-transfer-of-clubroot-resistance-genes-to-brassica-napus-using-distant-hybridization
#17
Yaping Liu, Aixia Xu, Fenghao Liang, Xueqin Yao, Yang Wang, Xia Liu, Yan Zhang, Jazira Dalelhan, Bingbing Zhang, Mengfan Qin, Zhen Huang, Lei Shaolin
Clubroot is an economically important disease affecting plants in the family Cruciferae worldwide. In this study, a collection of 50 Cruciferae accessions was screened using Plasmodiophora brassicae pathotype 4 in China. Eight of these demonstrated resistance, including three Chinese cabbages, two cabbages, one radish, one kale, and one Brassica juncea . The three clubroot-resistant Chinese cabbages (1003, 1007 and 1008) were then used to transfer the clubroot resistance genes to B. napus by distant hybridization combined with embryo rescue...
March 2018: Breeding Science
https://www.readbyqxmd.com/read/29875606/induction-of-apomixis-by-dimethyl-sulfoxide-dmso-and-genetic-identification-of-apomictic-plants-in-cassava
#18
Xia Chen, Hang-Gui Lai, Qi Sun, Jin-Ping Liu, Song-Bi Chen, Wen-Li Zhu
Apomixis, or asexual seed formation, is of great value for plant breeding and seed production, and is desirable in modern agriculture, but natural apomixis occurs in cassava at very low frequency. In present study, apomixis was induced by the treatments of female flower buds with 1%, 1.5% and 2% (v/v) dimethyl sulfoxide (DMSO) and the results showed that 1.5% DMSO treatment was most effective for the induction of apomictic seed formation in cassava cultivar SC5 with the highest percentages of fruit set and true apomictic seeds...
March 2018: Breeding Science
https://www.readbyqxmd.com/read/29875605/mapping-of-fruit-length-related-qtls-in-interspecific-cross-capsicum-annuum-l-%C3%A3-capsicum-galapagoense-hunz-of-chilli
#19
Konana Arjun, Major S Dhaliwal, Salesh K Jindal, Bashasab Fakrudin
Fruit length in chilli is quantitatively inherited trait and selection based on phenotypic performance is tedious and time consuming. To detect QTLs determining fruit length in Capsicum spp., an interspecific F2 mapping population was developed from the cross of C. annuum L. cv. 'FL 201' with C. galapagoense Hunz. accession 'TC 07245'. Fruit length in this cross showed a quantitative inheritance with the population depicting a symmetric distribution in histogram. To map quantitative trait loci (QTLs) for fruit length 400 SSR markers were surveyed on the parental genotypes but only 28 markers were observed to be polymorphic indicating less genetic diversity between the two Capsicum species...
March 2018: Breeding Science
https://www.readbyqxmd.com/read/29873892/is-population-genetic-structure-of-vascular-plants-shaped-more-by-ecological-or-geographical-factors-a-study-case-on-the-mediterranean-endemic-centaurea-filiformis-asteraceae
#20
Emmanuele Farris, Rossella Filigheddu, Giulia Mameli, Vito Falanga, Isabella Vanetti, Leonardo Rosati, Giorgio Binelli
THE RESEARCH CONDUCTED INCLUDING ITS RATIONALE: All known populations of the Sardinian endemic Centaurea filiformis Viv. (Asteraceae) were studied in order to understand the impact of both geographical and ecological factors on the genetic structuring of this species. CENTRAL METHODS APPLIED: Fourteen populations and 234 individuals were sampled. The demographic structure of the populations and the reproductive ecology were estimated in 28 plots. Population genetic analyses were based on SSR markers...
June 6, 2018: Plant Biology
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