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https://www.readbyqxmd.com/read/28820491/mapping-of-qtls-for-seed-phorbol-esters-a-toxic-chemical-in-jatropha-curcas-l
#1
Kitiya Amkul, Kularb Laosatit, Prakit Somta, Sangrea Shim, Suk-Ha Lee, Patcharin Tanya, Peerasak Srinives
Jatropha (Jatropha curcas L.) is an oil-bearing plant that has potential to be cultivated as a biodiesel crop. The seed cake after oil extraction has 40-50% protein that can be used in animal feeds. A major limitation in utilizing the cake is the presence of phorbol esters (PE), a heat-tolerant toxic chemical. To identify the quantitative trait loci (QTLs) for PE, we constructed a genetic linkage map from an F₂ population of 95 individuals from a cross "Chai Nat" × "M10" using 143 simple sequence repeat (SSR) markers...
August 18, 2017: Genes
https://www.readbyqxmd.com/read/28818380/molecular-cloning-and-expression-analysis-of-duplicated-polyphenol-oxidase-genes-reveal-their-functional-differentiations-in-sorghum
#2
Song Yan, Sujuan Li, Guowei Zhai, Ping Lu, Hui Deng, Shan Zhu, Renliang Huang, Jianfeng Shao, Yuezhi Tao, Guihua Zou
Polyphenol oxidase (PPO) is believed to play a role in plant growth, reproduction, and resistance to pathogens and pests. PPO causes browning of grains in cereals. In this study, genetic mapping of sorghum grain for phenol color reaction (PHR) was performed using a recombinant inbred line population. Only one locus was detected between SSR markers SM06072 and Xtxp176 on chromosome 6. Two linked orthologous genes (Sb06PPO1 and Sb06PPO2) within the mapped region were discovered and cloned. Transformation experiments using Nipponbare (a PHR negative rice cultivar) showed that Sb06PPO1 from LTR108 and two Sb06PPO2 alleles from both varieties could complement Nipponbare, whereas Sb06PPO1 from 654 could not...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28814406/comparative-epigenetic-and-genetic-spatial-structure-of-the-perennial-herb-helleborus-foetidus-isolation-by-environment-isolation-by-distance-and-functional-trait-divergence
#3
Carlos M Herrera, Mónica Medrano, Pilar Bazaga
PREMISE OF THE STUDY: Epigenetic variation can play a role in local adaptation; thus, there should be associations among epigenetic variation, environmental variation, and functional trait variation across populations. This study examines these relationships in the perennial herb Helleborus foetidus (Ranunculaceae). METHODS: Plants from 10 subpopulations were characterized genetically (AFLP, SSR markers), epigenetically (MSAP markers), and phenotypically (20 functional traits)...
August 16, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/28813039/comparative-assessment-of-ssr-and-snp-markers-for-inferring-the-population-genetic-structure-of-the-common-fungus-armillaria-cepistipes
#4
T Tsykun, C Rellstab, C Dutech, G Sipos, S Prospero
During the last years, simple sequence repeats (SSRs, also known as microsatellites) and single-nucleotide polymorphisms (SNPs) have become the most popular molecular markers for describing neutral genetic variation in populations of a wide range of organisms. However, only a limited number of studies has focused on comparing the performance of these two types of markers for describing the underlying genetic structure of wild populations. Moreover, none of these studies targeted fungi, the group of organisms with one of the most complex reproductive strategies...
August 16, 2017: Heredity
https://www.readbyqxmd.com/read/28812021/association-analysis-of-arsenic-induced-straighthead-in-rice-oryza-sativa-l-based-on-the-selected-population-with-a-modified-model
#5
Xiaobai Li, Biaolin Hu, Xuhao Pan, Ning Zhang, Dianxing Wu
A rice physiological disorder makes mature panicle keep erect with empty grains termed as "straighthead." Straighthead causes yield losses and is a serious threat to rice production worldwide. Here, a new study of association mapping was conducted to identify QTL involved in straighthead. A subset of 380 accessions was selected from the USDA rice core collection and genotyped with 72 genome-wide SSR markers. An optimal model implemented with principle components (PCs) was used in this association mapping. As a result, five markers were identified to be significantly associated with straighthead...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28810137/time-gated-luminescence-imaging-of-singlet-oxygen-photoinduced-by-fluoroquinolones-and-functionalized-graphenes-in-daphnia-magna
#6
Tianlie Luo, Jingwen Chen, Bo Song, Hua Ma, Zhiqiang Fu, Willie J G M Peijnenburg
Singlet oxygen ((1)O2) can be photogenerated by photoactive xenobiotics and is capable of causing adverse effects due to its electrophilicity and its high reactivity with biological molecules. Detection of the production and distribution of (1)O2 in living organisms is therefore of great importance. In this study, a luminescent probe ATTA-Eu(3+) combined with time-gated luminescence imaging was adopted to detect the distribution and temporal variation of (1)O2 photoinduced by fluoroquinolone antibiotics and carboxylated/aminated graphenes in Daphnia magna...
July 29, 2017: Aquatic Toxicology
https://www.readbyqxmd.com/read/28800082/complete-chloroplast-genome-sequence-and-phylogenetic-analysis-of-the-medicinal-plant-artemisia-annua
#7
Xiaofeng Shen, Mingli Wu, Baosheng Liao, Zhixiang Liu, Rui Bai, Shuiming Xiao, Xiwen Li, Boli Zhang, Jiang Xu, Shilin Chen
The complete chloroplast genome of Artemisia annua (Asteraceae), the primary source of artemisinin, was sequenced and analyzed. The A. annua cp genome is 150,995 bp, and harbors a pair of inverted repeat regions (IRa and IRb), of 24,850 bp each that separate large (LSC, 82,988 bp) and small (SSC, 18,267 bp) single-copy regions. Our annotation revealed that the A. annua cp genome contains 113 genes and 18 duplicated genes. The gene order in the SSC region of A. annua is inverted; this fact is consistent with the sequences of chloroplast genomes from three other Artemisia species...
August 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28799009/genome-wide-survey-and-characterization-of-fatty-acid-desaturase-gene-family-in-brassica-napus-and-its-parental-species
#8
Yufei Xue, Baojun Chen, Rui Wang, Aung Naing Win, Jiana Li, Yourong Chai
Rapeseed (Brassica napus) is an important oilseed crop worldwide, and fatty acid (FA) compositions determine the nutritional and economic value of its seed oil. Fatty acid desaturases (FADs) play a pivotal role in regulating FA compositions, but to date, no comprehensive genome-wide analysis of FAD gene family in rapeseed and its parent species has been reported. In this study, using homology searches, 84, 45, and 44 FAD genes were identified in rapeseed, Brassica rapa, and Brassica oleracea genomes, respectively...
August 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28798752/qtl-analysis-and-nested-association-mapping-for-adult-plant-resistance-to-powdery-mildew-in-two-bread-wheat-populations
#9
Yan Ren, Weixiu Hou, Caixia Lan, Bhoja R Basnet, Ravi P Singh, Wei Zhu, Xiyong Cheng, Dangqun Cui, Feng Chen
CIMMYT wheat (Triticum aestivum L.) lines Francolin#1 and Quaiu#3 displayed effective and stable adult plant resistance (APR) to Chinese Blumeria graminis f. sp. tritici isolates in the field. To elucidate their genetic basis of resistance, two recombinant inbred line (RIL) populations of their crosses with Avocet, the susceptible parent, were phenotyped in Zhengzhou and Shangqiu in the 2014-2015 and 2015-2016 cropping seasons. These populations were also genotyped with SSR (simple sequence repeat markers) and DArT (diversity arrays technology) markers...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28798292/14-5%C3%A2-ghz-passive-harmonic-mode-locking-in-a-dispersion-compensated-tm-doped-fiber-laser
#10
Yazhou Wang, Jianfeng Li, Kundong Mo, Yanyan Wang, Fei Liu, Yong Liu
We demonstrate a high repetition rate passive harmonic mode-locking (HML) based on nonlinear polarization evolution (NPE) technique in a Tm-doped ring fiber laser cavity. Small net anomalous cavity dispersion based on dispersion compensation benefited the generation of high repetition rate HML due to the low soliton splitting threshold. Stable HML with a repetition rate of up to 14.5 GHz and a super-mode suppression (SSR) of 19 dB was obtained at the center wavelength of 1982.3 nm, which is about ten times of state of the art at 2 μm band mode-locking fiber laser to our best knowledge...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28797302/development-and-characterization-of-microsatellite-markers-for-diploid-populations-of-the-wind-pollinated-herb-mercurialis-annua
#11
Ana Paula Machado, John R Pannell, Jeanne Tonnabel
OBJECTIVE: Mercurialis annua is a wind-pollinated annual plant that has long been used as a model for the study of ploidy and sexual-systems evolution. However, no molecular markers are yet available for genetic studies of its diploid populations. Here, we develop and characterize a set of eight polymorphic microsatellite markers for diploid dioecious M. annua. RESULTS: Following an SSR-enrichment protocol, 13 microsatellite markers were proposed, eight of which yielded successful amplification and polymorphism...
August 10, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28791211/development-and-characterization-of-polymorphic-est-based-ssr-markers-in-barley-hordeum-vulgare
#12
Won-Sam Jo, Hye-Yeong Kim, Kyung-Min Kim
In barley, breeding using good genetic characteristics can improve the quality or quantity of crop characters from one generation to the next generation. The development of effective molecular markers in barley is crucial for understanding and analyzing the diversity of useful alleles. In this study, we conducted genetic relationship analysis using expressed sequence tag-simple sequence repeat (EST-SSR) markers for barley identification and assessment of barley cultivar similarity. Seeds from 82 cultivars, including 31 each of naked and hulled barley from the Korea Seed and Variety Service and 20 of malting barley from the RDA-Genebank Information Center, were analyzed in this study...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28791207/development-of-ssr-markers-from-transcriptomes-for-orinus-poaceae-an-endemic-of-the-qinghai-tibetan-plateau
#13
Yu-Ping Liu, Xu Su, Wen-Chun Luo, Ting Lv, Ke-Long Chen, A J Harris, Sayed Afzal Shah
PREMISE OF THE STUDY: Transcriptomes were used to develop microsatellite markers for the plant genus Orinus (Poaceae), which comprises three species of grasses (O. thoroldii, O. kokonoricus, and O. intermedius) that are widely distributed in the Qinghai-Tibetan Plateau. METHODS AND RESULTS: Primer pairs were developed for 16 high-quality simple sequence repeats (SSRs) using transcriptomes. SSRs were amplified in 248 individuals representing the three species of Orinus; the number of alleles per locus ranged from one to seven, with an average of 2...
July 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28791205/development-of-est-derived-microsatellite-markers-in-the-aquatic-macrophyte-ranunculus-bungei-ranunculaceae
#14
Zhigang Wu, Jinwei Wu, Yalin Wang, Hongwei Hou
PREMISE OF THE STUDY: Microsatellite or simple sequence repeat (SSR) markers were developed to investigate the influence of ecological factors on gene flow and spatial genetic structuring of the submerged plant Ranunculus bungei (Ranunculaceae), which is regarded as an important species for understanding how plants adapt to an aquatic environment. METHODS AND RESULTS: Twenty-two microsatellite loci were identified from an expressed sequence tag (EST) library. The number of alleles per locus ranged from one to five, and the expected heterozygosity varied from 0...
July 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28791202/transcriptomic-analysis-genic-ssr-development-and-genetic-diversity-of-proso-millet-panicum-miliaceum-poaceae
#15
Siyu Hou, Zhaoxia Sun, Yaoshen Li, Yijie Wang, Hubin Ling, Guofang Xing, Yuanhuai Han, Hongying Li
PREMISE OF THE STUDY: Proso millet (Panicum miliaceum; Poaceae) is a minor crop with good nutritional qualities and strong tolerance to drought stress and soil infertility. However, studies on genetic diversity have been limited due to a lack of efficient genetic markers. METHODS: Illumina sequencing technology was used to generate short read sequences of proso millet, and de novo transcriptome assemblies were used to develop a de novo assembly of proso millet. Genic simple sequence repeat (SSR) markers were identified and used to detect polymorphism among 56 accessions...
July 2017: Applications in Plant Sciences
https://www.readbyqxmd.com/read/28769972/the-first-molecular-identification-of-an-olive-collection-applying-standard-simple-sequence-repeats-and-novel-expressed-sequence-tag-markers
#16
Soraya Mousavi, Roberto Mariotti, Luca Regni, Luigi Nasini, Marina Bufacchi, Saverio Pandolfi, Luciana Baldoni, Primo Proietti
Germplasm collections of tree crop species represent fundamental tools for conservation of diversity and key steps for its characterization and evaluation. For the olive tree, several collections were created all over the world, but only few of them have been fully characterized and molecularly identified. The olive collection of Perugia University (UNIPG), established in the years' 60, represents one of the first attempts to gather and safeguard olive diversity, keeping together cultivars from different countries...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28769959/introgression-of-black-rot-resistance-from-brassica-carinata-to-cauliflower-brassica-oleracea-botrytis-group-through-embryo-rescue
#17
Brij B Sharma, Pritam Kalia, Dinesh Singh, Tilak R Sharma
Black rot caused by Xanthomonas campestris pv. campestris (Xcc) is a very important disease of cauliflower (Brassica oleracea botrytis group) resulting into 10-50% yield losses every year. Since there is a dearth of availability of resistance to black rot disease in B. oleracea (C genome), therefore exploration of A and B genomes was inevitable as they have been reported to be potential reservoirs of gene(s) for resistance to black rot. To utilize these sources, interspecific hybrid and backcross progeny (B1) were generated between cauliflower "Pusa Sharad" and Ethiopian mustard "NPC-9" employing in vitro embryo rescue technique...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28769940/genome-wide-discovery-of-microsatellite-markers-from-diploid-progenitor-species-arachis-duranensis-and-a-ipaensis-and-their-application-in-cultivated-peanut-a-hypogaea
#18
Chuanzhi Zhao, Jingjing Qiu, Gaurav Agarwal, Jiangshan Wang, Xuezhen Ren, Han Xia, Baozhu Guo, Changle Ma, Shubo Wan, David J Bertioli, Rajeev K Varshney, Manish K Pandey, Xingjun Wang
Despite several efforts in the last decade toward development of simple sequence repeat (SSR) markers in peanut, there is still a need for more markers for conducting different genetic and breeding studies. With the effort of the International Peanut Genome Initiative, the availability of reference genome for both the diploid progenitors of cultivated peanut allowed us to identify 135,529 and 199,957 SSRs from the A (Arachis duranensis) and B genomes (Arachis ipaensis), respectively. Genome sequence analysis showed uneven distribution of the SSR motifs across genomes with variation in parameters such as SSR type, repeat number, and SSR length...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28764649/genetic-structure-and-relationships-within-and-between-cultivated-and-wild-korarima-aframomum-corrorima-braun-p-c-m-jansen-in-ethiopia-as-revealed-by-simple-sequence-repeat-ssr-markers
#19
Dagmawit Chombe, Endashaw Bekele, Tomas Bryngelsson, Abel Teshome, Mulatu Geleta
BACKGROUND: Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] is a spice crop native to Ethiopia. Understanding the extent and partitioning of diversity within and among crop landraces and their wild relatives is among the first steps in conserving and measuring their genetic potential. The present study is aimed at characterizing the population genetic structure and relationships between cultivated and wild korarima in the southwestern part of Ethiopia. RESULTS: We analyzed a total of 195 individuals representing seven wild and fourteen cultivated populations...
August 1, 2017: BMC Genetics
https://www.readbyqxmd.com/read/28764428/diffuse-ultrasonic-backscatter-using-a-multi-gaussian-beam-model
#20
Xiongbing Li, Yongfeng Song, Andrea P Arguelles, Joseph A Turner
Diffuse ultrasonic backscatter is widely used to evaluate microstructural parameters of heterogeneous materials. Recent singly scattered response (SSR) models utilize a single-Gaussian beam (SGB) assumption which is expected to have limitations. Following a similar formalism, a model is presented using a multi-Gaussian beam (MGB) assumption to characterize the transducer beam for longitudinal-to-longitudinal scattering at normal incidence through an interface with arbitrary curvature. First, the Wigner transform of the transducer field is defined using conjugate double-layer MGB expressions...
July 2017: Journal of the Acoustical Society of America
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