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A Mothes, S Léotard, I Nicolle, A Smets, D Chirio, C Rotomondo, F Tiger, P Del Giudice, C Perrin, D Néri, C Foucault, M Della Guardia, H Hyvernat, P-M Roger
BACKGROUND: The use of rapid microbiological tests is supported by antimicrobial stewardship policies. Targeted antibiotic therapy (TAT) for community-acquired pneumonia (CAP) with positive urinary antigen test (UAT) has been associated with a favorable impact on outcome. We aimed to determine the factors associated with TAT prescription. PATIENTS AND METHODS: We conducted a retrospective multicenter study including all patients presenting with CAP and positive UAT for Streptococcus pneumoniae or Legionella pneumophila from January 2010 to December 2013...
July 1, 2016: Médecine et Maladies Infectieuses
Artur Yakimovich, Yauhen Yakimovich, Michael Schmid, Jason Mercer, Ivo F Sbalzarini, Urs F Greber
Viruses spread between cells, tissues, and organisms by cell-free and cell-cell mechanisms, depending on the cell type, the nature of the virus, or the phase of the infection cycle. The mode of viral transmission has a large impact on disease development, the outcome of antiviral therapies or the efficacy of gene therapy protocols. The transmission mode of viruses can be addressed in tissue culture systems using live-cell imaging. Yet even in relatively simple cell cultures, the mechanisms of viral transmission are difficult to distinguish...
January 2016: MSphere
Piotr Tetlak, Christiane Ruedl
The Clec9A-diphtheria toxin receptor (DTR) transgenic mouse strain provides a robust animal model to study the function of lymphoid organ-resident CD8(+) dendritic cells (DCs) and nonlymphoid organ-specific CD103(+) DCs in infectioous diseases and inflammation. Here we describe some basic protocols for CD8(+)/CD103(+) DC isolation, for their in vivo depletion, and for their characterization by multi-color flow cytometry analysis. As an example for in vivo functional characterization of this DC subset, we present here the experimental cerebral malaria model...
2016: Methods in Molecular Biology
Dragutin Arsić, Dragan R Milovanović, Arbresa Bedzeti Ferati, Zoran Prokić, Vladan Vlajković, Kenan Ferati, Jovana Arsić
AIM: This study investigated the cumulative incidence of Chlamydia trachomatis infection in women treated in gynaecology departments of healthcare facilities in two towns in Serbia and one town in the Former Yugoslav Republic (FYR) of Macedonia, including their medical records in public health reports. METHODS: A cross-sectional observational research design with retrospective data collection during a five-year period (2008-2012) originated from women treated as in- and out-patients...
March 2016: Central European Journal of Public Health
Sasidharanpillai Sabeena, Parvati Bhat, Veena Kamath, Mary Mathew, Sushama Aswathyraj, Santhosha Devadiga, Suresha Prabhu, Maity Hindol, Akhil Chameetachal, Anjana Krishnan, Govindakarnavar Arunkumar
BACKGROUND: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. OBJECTIVE: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
Aladin Carovac, Sandra Vegar Zubovic, Marklena Carovac, Irmina Sefic Pasic
OBJECTIVES: The aim of this study was to determine sensitivity, specificity, and predictive values of sonographically demonstrated ureteral dilatation in detecting vesicoureteral reflux (VUR). METHODS: Ethical approval from the Ethical Committee of Clinical Center University of Sarajevo and parental consent were obtained for this prospective study involving 120 children with history of urinary tract infections (UTIs). Ultrasound examination included the evaluation of the urinary tract, with a special emphasis on evaluation of ureteral dilatation...
October 2015: Acta Informatica Medica: AIM
P-M Roger, J Tabutin, V Blanc, S Léotard, P Brofferio, F Léculé, B Redréau, E Bernard
BACKGROUND: Care to patients with prosthetic joint infections (PJI) is provided after pluridisciplinary collaboration, in particular for complex presentations. Therefore, to carry out an audit in PJI justifies using pluridisciplinary criteria. We report an audit for hip or knee PJI, with emphasis on care homogeneity, length of hospital stay (LOS) and mortality. PATIENTS AND METHODS: Fifteen criteria were chosen for quality of care: 5 diagnostic tools, 5 therapeutic aspects, and 5 pluridisciplinary criteria...
June 2015: Médecine et Maladies Infectieuses
Sandra Vegar-Zubović, Spomenka Kristić, Aladin Čarovac, Irmina Sefić-Pašić, Amra Džananović, Danka Miličić-Pokrajac, Lincender Lidija
AIM: To analyze the usefulness of five ultrasound parameters (ureteral dilatation, renal pelvis dilatation, renal parenchyma width reduction, calyceal dilatation, and urothelial reaction) in detecting vesicoureteral reflux (VUR). METHODS: The study included 101 patients with diagnosed and therapeutically treated urinary infection. The ultrasound examination and voiding urosonography (VUS) were carried out according to a standard protocol. In the group of patients with proven VUR the presence of the indirect ecomorphological signs of VUR was evaluated...
August 2014: Medicinski Glasnik
D Martiny, P Cremagnani, A Gaillard, V Y Miendje Deyi, G Mascart, A Ebraert, S Attalibi, A Dediste, O Vandenberg
The mutualisation of analytical platforms might be used to address rising healthcare costs. Our study aimed to evaluate the feasibility of networking a unique matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS) system for common use in several university hospitals in Brussels, Belgium. During a one-month period, 1,055 successive bacterial isolates from the Brugmann University Hospital were identified on-site using conventional techniques; these same isolates were also identified using a MALDI-TOF MS system at the Porte de Hal Laboratory by sending target plates and identification projects via transportation and the INFECTIO_MALDI software (Infopartner, Nancy, France), respectively...
May 2014: European Journal of Clinical Microbiology & Infectious Diseases
Rodrigo Ríos, Salim Mattar, Marco González
OBJECTIVE: Analysing scientific production in the area of infectious diseases in Colombia, 2000-2009. MATERIAL AND METHODS: A literature search was made in category A journals in Colciencias' Publindex. The journals were: Biomédica, Revista de Salud Pública (Universidad Nacional), Colombia Médica, Ciencias Pecuarias, Latreia, MVZ Córdoba, Revista de Medicina (Universidad Nacional) and Infectio. RESULTS: 2,963 publications were found; 2,744 (92...
April 2011: Revista de Salud Pública
Deborah Money, Simon Dobson, Lesley Cole, Eda Karacabeyli, Edith Blondel-Hill, Ruth Milner, Eva Thomas
OBJECTIVE: To evaluate the sensitivity, specificity, and feasibility of a rapid real-time polymerase chain reaction (PCR) test for group B streptococcus (GBS) completed during labour, compared with the standard culture test performed at 35 to 37 weeks' gestation. METHODS: Women presenting to the maternity unit for term vaginal delivery had two vaginal/rectal samples collected. One swab was tested using a rapid PCR method (IDI-Strep B, Infectio Diagnostic [IDI] Inc...
September 2008: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
K Oberdorfer, S Pohl, M Frey, K Heeg, C Wendt
The aim of the present study was to determine the diagnostic value of a single-locus real-time polymerase chain reaction (PCR) recently proposed for rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) from clinical samples (IDI-MRSA; Infectio Diagnostic, Sainte-Foy, Québec, Canada). This test, which was developed on the basis of studies of the sequence analysis of the mecA gene carried by staphylococcal cassette chromosome mec (SCCmec), was used to screen nasal swabs of 320 intensive care unit (ICU) patients at admission...
October 2006: European Journal of Clinical Microbiology & Infectious Diseases
V G Nesterenko, V A Akovbian, E N Semenova, L M Vavilova, T I Iudina
During the last STDs'epidemic in Russia (1994 - 2004) over 2.7 min people have been infected with syphilis. At present the structure of syphilis morbidity is characterized by 37% of latent forms, including 1.5% late latency. The increased level of late latency may result from: an ever-growing number of those with asymptomatic syphilis; disorderly and self-dependent usage of antibacterial preparations; spread of the virus chronic infections (herpes, hepatitis B, C) altering the macroorganism immune response; alcohol and drug abuse which decreases the efficacy of specific therapy...
July 2006: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii
Emma J Bishop, Elizabeth A Grabsch, Susan A Ballard, Barrie Mayall, Shirley Xie, Rhea Martin, M Lindsay Grayson
The IDI-MRSA assay (Infectio Diagnostic, Inc., Sainte-Foy, Quebec, Canada) with the Smart Cycler II rapid DNA amplification system (Cepheid, Sunnyvale, CA) appears to be sensitive and specific for the rapid detection of nasal colonization by methicillin-resistant Staphylococcus aureus (MRSA). We assessed the sensitivity and specificity of this assay under conditions in which both the nose and cutaneous groin specimens were analyzed together and compared the accuracy of this PCR approach to that when these specimens were tested separately and by culture assays in an inpatient population with known high rates (12 to 15%) of MRSA colonization...
August 2006: Journal of Clinical Microbiology
Xi-yang Wang, Jian-zhong Hu, Wen-he Liu, Kang-hua Li
OBJECTIVE: To retrospectively explore the diagnosis and treatment of postoperative lumbar intervertebral disc infection. METHODS: Twelve patients who suffered from postoperative lumbar intervertebral disc infection between April 1995 and September 2004 were confirmed doubtlessly by the diagnosis. They were treated with early immobilization combined with early and sufficient antibiotics and adequate corticosteroid. Patients with severe symptoms were managed by percutaneous puncture and washing discectomy such as rising temperature and perivertebral muscle spasm besides nostalgia, and early debridement of all necrotic tissue was done...
February 2006: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
S Ranque, B Marchou, D Malvy, E Adehossi, R Laganier, H Tissot-Dupont, A Lotte, S Dydymsky, J Durant, J-P Stahl, A Bosseray, J Gaillat, A Sotto, C Cazorla, J-M Ragneau, P Brouqui, J Delmont et al.
BACKGROUND: Data about anti-malarial drugs prescription practices in Europe and the safety of imported malaria treatments are scanty. In 1999, a French consensus development conference published guidelines for the prevention and treatment of imported P. falciparum malaria. The impact of these guidelines has not been evaluated. AIM: To investigate the impact of these guidelines on the prescription of anti-malarials, and to evaluate the incidence of acute drug events (ADEs) leading to discontinuation of treatment...
October 2005: QJM: Monthly Journal of the Association of Physicians
Wade K Aldous, June I Pounder, Joann L Cloud, Gail L Woods
Six methods of extracting Mycobacterium tuberculosis DNA from sputum for testing by quantitative PCR were compared: Tris-EDTA (TE) buffer, PrepMan Ultra, 2% sodium dodecyl sulfate (SDS)-10% Triton X with and without sonication, Infectio Diagnostics, Inc. (IDI) lysing tubes, and QIAGEN QIAamp DNA mini kit; all included a 15-min boiling step. Pooled digested and decontaminated sputum was spiked with M. tuberculosis ATCC 27294. Each extraction method was repeated eight times. Quantitative PCR was performed on the Smart Cycler and Rotor-Gene 3000 using primers targeting an 83-bp fragment of IS6110...
May 2005: Journal of Clinical Microbiology
David K Warren, Robert S Liao, Liana R Merz, Michael Eveland, W Michael Dunne
Screening for colonization with methicillin-resistant Staphylococcus aureus (MRSA) is a key aspect of infection control to limit the nosocomial spread of this organism. Current methods for the detection of MRSA in clinical microbiology laboratories, including molecularly based techniques, require a culture step and the isolation of pure colonies that result in a minimum of 20 to 24 h until a result is known. We describe a qualitative in vitro diagnostic test for the rapid detection of MRSA directly from nasal swab specimens (IDI-MRSA; Infectio Diagnostic, Inc...
December 2004: Journal of Clinical Microbiology
H Dele Davies, Mark A Miller, Sebastian Faro, Dan Gregson, Sue C Kehl, Jeanne A Jordan
BACKGROUND: Current prevention of infection due to group B Streptococcus (GBS) involves giving intrapartum antibiotics to women on the basis of either antenatal culture colonization status or presence of risk factors. METHODS: We prospectively compared the performance characteristics of a rapid molecular diagnostic test (IDI-Strep B; Infectio Diagnostic) with culture for intrapartum GBS detection after 36 weeks' gestation in 5 North American centers during the period September 2001-May 2002...
October 15, 2004: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Sékéné Badiaga, Karine Barrau, Philippe Brouqui, Jacques Durant, Denis Malvy, François Janbon, Eric Bonnet, Annick Bosseray, Albert Sotto, Dominique Peyramont, Serge Dydymski, Celine Cazorla, Hervé Tolou, Jean Paul Durant, Jean Delmont et al.
Dengue fever (DF) is a mosquito-transmitted acute disease caused by any of four dengue flavivirus serotypes (DEN-1, DEN-2, DEN-3, and DEN-4) which is becoming a major public health problem in intertropical areas.1 DF is increasingly observed in febrile travelers returning from tropical areas,2 especially those returning from the Caribbean islands and Southeast Asia, but it is rarely diagnosed in travelers returning from Africa.3-8 Diagnosis is often serologic, and in most cases is not confirmed; virus isolation remains exceptional...
September 2003: Journal of Travel Medicine
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