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https://www.readbyqxmd.com/read/28437180/a-novel-drug-induced-hybrid-electrospun-pcl-cell-derived-ecm-scaffold-for-liver-tissue-engineering
#1
Rhiannon Grant, David Hay, Anthony Callanan
Liver transplant is the only treatment option for patients with end-stage liver failure, however there are too few donor livers available for transplant. Whole organ tissue engineering presents a potential solution to the problem of rapidly escalating donor liver shortages world-wide. A major challenge for liver tissue engineers is the creation of a hepatocyte micro-environment; a niche in which liver cells can survive and function optimally. While polymers and decellularized tissues pose an attractive option for scaffold manufacturing, neither alone has thus far proved sufficient...
February 24, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28436474/interactions-of-amtn-odam-and-scpppq1-proteins-of-a-specialized-basal-lamina-that-attaches-epithelial-cells-to-tooth-mineral
#2
Aurélien Fouillen, Juliana Dos Santos Neves, Charline Mary, Jean-Daniel Castonguay, Pierre Moffatt, Christian Baron, Antonio Nanci
A specialized basal lamina (sBL) mediates adhesion of certain epithelial cells to the tooth. It is distinct because it does not contain collagens type IV and VII, is enriched in laminin-332, and includes three novel constituents called amelotin (AMTN), odontogenic ameloblast-associated (ODAM), and secretory calcium-binding phosphoprotein proline-glutamine rich 1 (SCPPPQ1). The objective of this study was to clarify the structural organization of the sBL. Fluorescence and immunogold labeling showed that the three proteins co-localize...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28433788/regulation-of-human-nucleus-pulposus-cells-by-peptide-coupled-substrates
#3
Devin T Bridgen, Bailey V Fearing, Liufang Jing, Johannah Sanchez-Adams, Megan C Cohan, Farshid Guilak, Jun Chen, Lori A Setton
Nucleus pulposus (NP) cells are derived from the notochord and differ from neighboring cells of the intervertebral disc in phenotypic marker expression and morphology. Adult human NP cells lose this phenotype and morphology with age in a pattern that contributes to progressive disc degeneration and pathology. Select laminin-mimetic peptide ligands and substrate stiffnesses were examined for their ability to regulate human NP cell phenotype and biosynthesis through the expression of NP-specific markers aggrecan, N-cadherin, collagen types I and II, and GLUT1...
April 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28432022/a-crosstalk-between-muscarinic-and-crf2-receptors-regulates-cellular-adhesion-properties-of-human-colon-cancer-cells
#4
M Pelissier-Rota, N T Chartier, B Bonaz, M R Jacquier-Sarlin
Patients with inflammatory bowel disease often suffer from chronic and relapsing intestinal inflammation that favor the development of colitis associated cancer. An alteration of the epithelial intestinal barrier function observed in IBD is supposed to be a consequence of stress. It has been proposed that corticotrophin-releasing factor receptor (CRF2), one of the two receptors of CRF, the principal neuromediator of stress, acts on cholinergic nerves to induce stress-mediated epithelial barrier dysfunction...
April 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28422363/the-occhiolino-occ-mutant-zebrafish-a-model-for-development-of-the-optical-function-in-the-biological-lens
#5
Masamoto Aose, Tor H Linbo, Owen Lawrence, Tadashi Senoo, David W Raible, John I Clark
BACKGROUND: Zebrafish visual function depends on quality optics. An F3 screen for developmental mutations in the zebrafish nervous system was conducted in wild-type (wt) AB zebrafish exposed to 3 mM of N-ethyl-N-nitrosourea (ENU). RESULTS: Mutant offspring, identified in an F3 screen, were characterized by a small pupil, resulting from retinal hypertrophy or hyperplasia and a small lens. Deficits in visual function made feeding difficult after hatching at approximately 5-6 dpf (days post fertilization)...
April 19, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28418226/serum-monomeric-laminin-%C3%AE-2-as-a-novel-biomarker-for-hepatocellular-carcinoma
#6
Hirofumi Kiyokawa, Hiroshi Yasuda, Ritsuko Oikawa, Chiaki Okuse, Nobuyuki Matsumoto, Hiroki Ikeda, Tsunamasa Watanabe, Hiroyuki Yamamoto, Fumio Itoh, Takehito Otsubo, Toru Yoshimura, Eisaku Yoshida, Masatoshi Nakagawa, Naohiko Koshikawa, Motoharu Seiki
The diagnosis of hepatocellular carcinoma (HCC) in the early stages is important for successful clinical management. Laminin (Ln)-γ2 expression has been reported in various types of malignant carcinomas. We recently developed a highly sensitive method to measure serum monomeric Ln-γ2 levels using a fully automated chemiluminescent immunoassay (CLIA). Using our CLIA, we evaluated its diagnostic value in sera from patients with chronic liver disease (CLD) and patients with hepatocellular carcinoma (HCC). Serum alpha-fetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP) were also examined in these subjects...
April 18, 2017: Cancer Science
https://www.readbyqxmd.com/read/28418174/hypoxia-upregulates-integrin-gene-expression-in-microvascular-endothelial-cells-and-promotes-their-migration-and-capillary-like-tube-formation
#7
Christina Befani, Panagiotis Liakos
Tissue hypoxia affects gene expression through the hypoxia-inducible transcription factors, HIF-1 and HIF-2, in both physiological and pathological angiogenesis. Angiogenesis is a complex response of endothelial cells integrating cell proliferation, migration, tube formation and their interaction with the extracellular matrix through integrin receptors. In this report, we studied the effect of hypoxia on the angiogenic functions of human microvascular endothelial cells (HMEC-1) as well as on expression of the angiogenic integrins αν β3 , αν β5 and α5 β1 ...
April 18, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28415472/supercritical-carbon-dioxide-extracted-extracellular-matrix-material-from-adipose-tissue
#8
Jun Kit Wang, Baiwen Luo, Vipra Guneta, Liang Li, Selin Ee Min Foo, Yun Dai, Timothy Thatt Yang Tan, Nguan Soon Tan, Cleo Choong, Marcus Thien Chong Wong
Adipose tissue is a rich source of extracellular matrix (ECM) material that can be isolated by delipidating and decellularizing the tissue. However, the current delipidation and decellularization methods either involve tedious and lengthy processes or require toxic chemicals, which may result in the elimination of vital proteins and growth factors found in the ECM. Hence, an alternative delipidation and decellularization method for adipose tissue was developed using supercritical carbon dioxide (SC-CO2) that eliminates the need of any harsh chemicals and also reduces the amount of processing time required...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28415467/poly-l-lactic-acid-and-polyurethane-nanofibers-fabricated-by-solution-blow-spinning-as-potential-substrates-for-cardiac-cell-culture
#9
Ewelina Tomecka, Michal Wojasinski, Elzbieta Jastrzebska, Michal Chudy, Tomasz Ciach, Zbigniew Brzozka
This paper presents a comparison and evaluation of cardiac cell proliferation on poly(l-lactic acid) (PLLA) and polyurethane (PU) nanofibrous mats fabricated by solution blow spinning (SBS). Three different cardiac cell lines: rat cardiomyoblasts (H9C2 line), human (HCM) and rat cardiomyocytes (RCM) were used for experiments. Cell morphology, orientation and proliferation were investigated on non-modified and protein-modified (fibronectin, collagen, gelatin, laminin, poly-l-lysine) surfaces of both types of nanofibers...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28412969/engagement-of-cellular-prion-protein-with-the-co-chaperone-hsp70-90-organizing-protein-regulates-the-proliferation-of-glioblastoma-stem-like-cells
#10
Rebeca Piatniczka Iglesia, Mariana Brandão Prado, Lilian Cruz, Vilma Regina Martins, Tiago Góss Santos, Marilene Hohmuth Lopes
BACKGROUND: Glioblastoma (GBM), a highly aggressive brain tumor, contains a subpopulation of glioblastoma stem-like cells (GSCs) that play roles in tumor maintenance, invasion, and therapeutic resistance. GSCs are therefore a promising target for GBM treatment. Our group identified the cellular prion protein (PrP(C)) and its partner, the co-chaperone Hsp70/90 organizing protein (HOP), as potential target candidates due to their role in GBM tumorigenesis and in neural stem cell maintenance...
April 17, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28412362/probing-the-acidic-residue-within-the-integrin-binding-site-of-laminin-511-that-interacts-with-the-metal-ion-dependent-adhesion-site-of-%C3%AE-6%C3%AE-1-integrin
#11
Yukimasa Taniguchi, Shaoliang Li, Mamoru Takizawa, Eriko Oonishi, Junko Toga, Emiko Yagi, Kiyotoshi Sekiguchi
Laminins are major cell-adhesive proteins of basement membranes that interact with integrins in a divalent cation-dependent manner. Laminin-511 consists of α5, β1, and γ1 chains, of which three laminin globular domains of the α5 chain (α5/LG1-3) and a Glu residue in the C-terminal tail of chain γ1 (γ1-Glu1607) are required for binding to integrins. However, it remains unsettled whether the Glu residue in the γ1 tail is involved in integrin binding by coordinating the metal ion in the metal ion-dependent adhesion site of β1 integrin (β1-MIDAS), or by stabilizing the conformation of α5/LG1-3...
April 12, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28404626/adenosine-formed-by-cd73-on-t-cells-inhibits-cardiac-inflammation-and-fibrosis-and-preserves-contractile-function-in-transverse-aortic-constriction-induced-heart-failure
#12
Christine Quast, Christina Alter, Zhaoping Ding, Nadine Borg, Jürgen Schrader
BACKGROUND: Structural damage during heart failure development leads to increased infiltration of leukocytes. Because purinergic signaling on immune cells may impact on the inflammatory response, we evaluated the role of ecto-5'-nucleotidase (CD73) on the development of heart failure after transverse aortic constriction (TAC) using global and T-cell-specific CD73(-)(/-) mice. METHODS AND RESULTS: Leukocytes infiltrating the failing heart were analyzed by a multistep enzymatic procedure over a period of 16 weeks using fluorescence-activated cell sorting...
April 2017: Circulation. Heart Failure
https://www.readbyqxmd.com/read/28393762/engineering-a-functional-three-dimensional-human-cardiac-tissue-model-for-drug-toxicity-screening
#13
Hong Fang Lu, Meng Leong, Tze Lim, Ying Ping Chua, Jia Kai Lim, Chan Du, Andrew C A Wan
Cardiotoxicity is one of the major reasons for clinical drug attrition. In vitro tissue models that can provide efficient and accurate drug toxicity screening are highly desired for preclinical drug development and personalized therapy. Here, we report the fabrication and characterization of a human cardiac tissue model for high throughput drug toxicity studies. Cardiac tissues were fabricated via cellular self-assembly of human transgene-free iPSC-derived cardiomyocytes in pre-fabricated polydimethylsiloxane molds...
April 10, 2017: Biofabrication
https://www.readbyqxmd.com/read/28392652/paraneoplastic-pemphigus-associated-with-a-malignant-thymoma-a-case-of-persistent-and-refractory-oral-ulcerations-following-thymectomy
#14
Jung Min Lim, Sang Eun Lee, Jimyung Seo, Do Young Kim, Takashi Hashimoto, Soo-Chan Kim
Paraneoplastic pemphigus is a rare, life-threatening autoimmune mucocutaneous blistering disease associated with underlying neoplasia, commonly lymphoproliferative tumors. Herein we report a case of paraneoplastic pemphigus with a unique autoantibody profile associated with a malignant thymoma. A 56-year-old female patient presented with relapsing oral ulcerations accompanied by erythematous papules and patches on her extremities for 2 months. Skin and mucosal biopsies identified interface dermatitis with lichenoid lymphocytic infiltration in the upper dermis...
April 2017: Annals of Dermatology
https://www.readbyqxmd.com/read/28391962/su9516-increases-%C3%AE-7%C3%AE-1-integrin-and-ameliorates-disease-progression-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy
#15
Apurva Sarathy, Ryan D Wuebbles, Tatiana M Fontelonga, Ashley R Tarchione, Lesley A Mathews Griner, Dante J Heredia, Andreia M Nunes, Suzann Duan, Paul D Brewer, Tyler Van Ry, Grant W Hennig, Thomas W Gould, Andrés E Dulcey, Amy Wang, Xin Xu, Catherine Z Chen, Xin Hu, Wei Zheng, Noel Southall, Marc Ferrer, Juan Marugan, Dean J Burkin
Duchenne muscular dystrophy (DMD) is a fatal muscle disease caused by mutations in the dystrophin gene, resulting in a complete loss of the dystrophin protein. Dystrophin is a critical component of the dystrophin glycoprotein complex (DGC), which links laminin in the extracellular matrix to the actin cytoskeleton within myofibers and provides resistance to shear stresses during muscle activity. Loss of dystrophin in DMD patients results in a fragile sarcolemma prone to contraction-induced muscle damage. The α7β1 integrin is a laminin receptor protein complex in skeletal and cardiac muscle and a major modifier of disease progression in DMD...
April 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28391159/inhibitory-effects-of-quercetin-on-the-progression-of-liver-fibrosis-through-the-regulation-of-nf-%C3%B0%C2%BAb-i%C3%B0%C2%BAb%C3%AE-p38-mapk-and-bcl-2-bax-signaling
#16
Rong Wang, Hai Zhang, Yuanyuan Wang, Fuxing Song, Yongfang Yuan
Quercetin, a natural flavonoid, has been used as a nutritional supplement for its anti-inflammatory and antioxidative properties. Quercetin was reported to exhibit a wide range of pharmacological properties, including its effect on anti-hepatic fibrosis. However, the anti-fibrotic mechanisms of quercetin have not been well-characterized to date. This study aimed to investigate the protective effects of quercetin on carbon tetrachloride (CCl4)-induced liver fibrosis in rats and to clarify its anti-hepatofibrotic mechanisms...
April 6, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28387652/pediatric-ependymoma-a-proteomics-perspective
#17
George Th Tsangaris, Chrissa Papathanasiou, Panagiotis G Adamopoulos, Andreas Scorilas, Constantinos E Vorgias, Neofytos Prodromou, Foteini Tzortzatou Stathopoulou, Dimitrios J Stravopodis, Athanasios K Anagnostopoulos
BACKGROUND/AIM: Proteomics based on high-resolution mass spectrometry (MS) is the tool of choice for the analysis of protein presence, modifications and interactions, with increasing emphasis on the examination of tumor tissues. Application of MS-based proteomics offers a detailed picture of tumor tissue characteristics, facilitating the appreciation of different tumor entities, whilst providing reliable and fast results for therapeutic marker targeting and prognostic factor assessment...
March 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28387057/hypoxia-enhanced-adhesion-of-red-blood-cells-in-microscale-flow
#18
Myeongseop Kim, Yunus Alapan, Anima Adhikari, Jane A Little, Umut A Gurkan
OBJECTIVES: The advancement of microfluidic technology has facilitated the simulation of physiological conditions of the microcirculation, such as oxygen tension, fluid flow, and shear stress in these devices. Here, we present a micro-gas exchanger integrated with microfluidics to study red blood cell (RBC) adhesion under hypoxic flow conditions mimicking post-capillary venules. METHODS: We simulated a range of physiological conditions and explored RBC adhesion to endothelial or sub-endothelial components (fibronectin, FN, or laminin, LN)...
April 7, 2017: Microcirculation: the Official Journal of the Microcirculatory Society, Inc
https://www.readbyqxmd.com/read/28369516/comprehensive-chromosome-screening-and-gene-expression-analysis-from-the-same-biopsy-in-human-preimplantation-embryos
#19
Diego Marin, Yujue Wang, Xin Tao, Richard T Scott, Nathan R Treff
STUDY QUESTION: Can simultaneous comprehensive chromosome screening (CCS) and gene expression analysis be performed on the same biopsy of preimplantation human embryos? SUMMARY ANSWER: For the first time, CCS and reliable gene expression analysis have been performed on the same human preimplantation embryo biopsy. WHAT IS KNOWN ALREADY: A single trophectoderm (TE) biopsy is routinely used for many IVF programs offering CCS for selection of only chromosomally normal embryos for transfer...
March 23, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28367924/5-hmf-attenuates-liver-fibrosis-in-ccl4-plus-alcohol-induced-mice-by-suppression-of-oxidative-stress
#20
Xin-Yue Han, Jun-Nan Hu, Zi Wang, Sheng-Nan Wei, Si-Wen Zheng, Ying-Ping Wang, Wei Li
The aim of this study was to investigate the effects of 5-hydroxymethyl-2-furfural (5-HMF) on liver fibrosis induced by carbon tetrachloride (CCl4) and alcohol. Male ICR mice were treated with CCl4 dissolved in olive oil (10% v/v, 2.5 μg/L) intraperitoneally (i.p.), and given at a dose of 2.5×10(-5) mg/kg B.W. twice a week for 7 wk. Concurrently, mice received drinking water with or without alcohol. The mice in treatment groups and positive control group were gavaged with 5-HMF (7.5, 15, and 30 mg/kg B.W...
2017: Journal of Nutritional Science and Vitaminology
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