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A D Schwartz, C L Hall, L E Barney, C C Babbitt, S R Peyton
Cells sense and respond to mechanical cues from the extracellular matrix (ECM) via integrins. ECM stiffness is known to enhance integrin clustering and response to epidermal growth factor (EGF), but we lack information on when or if these mechanosensitive growth factor receptors and integrins converge intracellularly. Towards closing this knowledge gap, we combined a biomaterial platform with transcriptomics, molecular biology, and functional assays to link integrin-mediated mechanosensing and epidermal growth factor receptor (EGFR) signaling...
June 2, 2018: Biomaterials
Andreia Hanada Otake, Renata de Freitas Saito, Ana Paula Marques Duarte, Alexandre Ferreira Ramos, Roger Chammas
Melanomas often accumulate gangliosides, sialic acid-containing glycosphingolipids found in the outer leaflet of plasma membranes, as disialoganglioside GD3 and its derivatives. Here, we have transfected the GD3 synthase gene (ST8Sia I) in a normal melanocyte cell line in order to evaluate changes in the biological behavior of non-transformed cells. GD3 -synthase expressing cells converted GM3 into GD3 and accumulated both GD3 and its acetylated form, 9-O-acetyl-GD3 . Melanocytes were rendered more migratory on laminin-1 surfaces...
June 13, 2018: Biochimica et Biophysica Acta
E Mammadova-Bach, T Rupp, C Spenlé, I Jivkov, P Shankaranarayanan, A Klein, L Pisarsky, A Méchine-Neuville, G Cremel, M Kedinger, O De Wever, N Ambartsumian, S Robine, E Pencreach, D Guenot, P Simon-Assmann, J G Goetz, G Orend, O Lefebvre
Tumor stroma remodeling is a key feature of malignant tumors and can promote cancer progression. Laminins are major constituents of basement membranes that physically separate the epithelium from the underlying stroma. By employing mouse models expressing high and low levels of the laminin α1 chain (LMα1), we highlighted its implication in a tumor-stroma crosstalk, thus leading to increased colon tumor incidence, angiogenesis and tumor growth. The underlying mechanism involves attraction of carcinoma-associated fibroblasts by LMα1, VEGFA expression triggered by the complex integrin α2β1-CXCR4 and binding of VEGFA to LM-111, which in turn promotes angiogenesis, tumor cell survival and proliferation...
June 15, 2018: Biology of the Cell
Aki Natsumi, Koji Sugawara, Makiko Yasumizu, Yukari Mizukami, Shigetoshi Sano, Akimichi Morita, Ralf Paus, Daisuke Tsuruta
Psoriasis is a complex chronic inflammatory skin disease characterized by epidermal thickening on the basis of increased keratinocyte proliferation and insufficient apoptosis. Laminins are important components of the basement membrane (BM) and impact on epidermal keratinocyte growth/apoptosis. Although several laminins are involved in the pathogenesis of psoriasis, it is still controversial about the expression patterns of laminin isoforms and which laminins are important in the development of psoriasis. Because laminin-511 and -332 are key BM components in human skin, and laminin-511 stimulates human hair follicle growth, we asked whether the BM zone in psoriasis shows any laminin-related abnormalities...
June 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
Hams Saeed Al-Zahrani, Saeed Al-Tala, Hussein Shaikh Ali Mohamoud, Bandar Ali Al-Shehri, Saeed Al-Fadhel, Ali Al-Qurashi, Ahmad Al-Bishri, Jumana Yousuf Al-Aama, Changsoo Kang, Regina C Betz, Musharraf Jelani
Non-Herlitz type of junctional epidermolysis bullosa (JEB-NH, OMIM 226650) is typically milder than Herlitz JEB (JEB-H, OMIM 226700).
June 13, 2018: Congenital Anomalies
M P Marinkovich
No abstract text is available yet for this article.
June 2018: British Journal of Dermatology
Joanna Włodek, Anna Pituch-Noworolska
The extracellular matrix (ECM) is a dynamic environment involved in the regulation of haematopoiesis. A crucial role of this structure is the promotion of proliferation, maturation, and differentiation of haematopoietic stem cells (HSC), and adhesion and migration of HSC in bone marrow. In the present study the effect of ECM proteins (fibronectin, collagens, laminin, thrombospondin, and vitronectin) on proliferation and apoptosis of acute lymphoblastic leukaemia cells isolated from acute lymphoblastic leukaemia (ALL) patients (<i><i><i><i><i>in vitro</i></i></i></i></i>) was assessed...
2018: Polish Journal of Pathology: Official Journal of the Polish Society of Pathologists
H L M Tucker, J Holdridge, C L M Parsons, R M Akers
It is established that the ovary and estrogen are essential to bovine mammary development with the onset of puberty. Recent studies have shown that ovariectomy in the very early prepubertal period, well before onset of puberty, also dramatically impairs mammary growth. Similarly, prepubertal heifers treated with the antiestrogen tamoxifen (TAM) also exhibit markedly impaired mammary growth in correspondence with reduced estrogen receptor α (ESR1) expression. Our objective was to evaluate the effect of TAM on the mammary stroma and specifically to determine if the reported decrease in mammary development was related to changes in TAM-induced alterations in the stroma surrounding the mammary parenchyma...
May 18, 2018: Domestic Animal Endocrinology
Abdelfattah M Attallah, Dalia Omran, Mohamed M Omran, Mohamed A Abdelrazek, Rania Zayed, Riham El Essawey, Sameh Saif, Azza Farid, Mohamed Hassany, Ayman Yosry, Ashraf Omar
INTRODUCTION AND AIM: The correlation between interleukin-28B (IL-28B) polymorphisms and chronic hepatitis C (CHC) progression is debatable. Here, we aimed to evaluate the relation between IL-28B C/T genotypes and the development of cirrhotic liver. Extracellular matrix (ECM) proteins, FibroScan and model for end-stage liver disease (MELD) were used to substantiate the severity of liver disease. MATERIAL AND METHODS: IL-28B rs12979860, liver stiffness and ECM proteins were assessed in 272 CHC patients...
July 2018: Annals of Hepatology
Yunxiao Zhu, Zdravka Cankova, Marta Iwanaszko, Sheridan Lichtor, Milan Mrksich, Guillermo A Ameer
The successful treatment of chronic dermal wounds, such as diabetic foot ulcers (DFU), depends on the development of safe, effective, and affordable regenerative tools that the surgeon can rely on to promote wound closure. Although promising, strategies that involve cell-based therapies and the local release of exogenous growth factors are costly, require very long development times, and result in modest improvements in patient outcome. We describe the development of an antioxidant shape-conforming regenerative wound dressing that uses the laminin-derived dodecapeptide A5G81 as a potent tethered cell adhesion-, proliferation-, and haptokinesis-inducing ligand to locally promote wound closure...
June 11, 2018: Proceedings of the National Academy of Sciences of the United States of America
André Vicente, Berit Byström, Mona Lindström, Ulf Stenevi, Fátima Pedrosa Domellöf
BACKGROUND: To study structural changes in naïve and surgically treated corneas of aniridia patients with advanced aniridia-related keratopathy (ARK). METHODS AND FINDINGS: Two naïve corneal buttons from patients with advanced ARK submitted to penetrating keratoplasty for the first time, one corneal button from an ARK patient that had undergone a keratolimbal allograft (KLAL), two corneal buttons from ARK patients who had previously undergone centered or decentered transplantation and were now retransplanted and two adult healthy donor control corneas were processed for immunohistochemistry...
2018: PloS One
Cao Zhou, Hong Yang, Zhao Wang, Gui-Yun Long, Dao-Chao Jin
White-backed planthopper, Sogatella furcifera (Horváth) (Hemiptera: Delphacidae), one of the main agricultural insect pests in China, is resistant to a wide variety of insecticides. We used transcriptome analysis to compare the expression patterns of resistance- and stress-response genes in S. furcifera subjected to imidacloprid, deltamethrin, and triazophos stress, to determine the molecular mechanisms of resistance to these insecticides. A comparative analysis of gene expression under imidacloprid, deltamethrin, and triazophos stress revealed 1,123, 841, and 316 upregulated unigenes, respectively, compared to the control...
June 8, 2018: Scientific Reports
Rieko Imae, Hiroshi Manya, Hiroki Tsumoto, Kenji Osumi, Tomohiro Tanaka, Mamoru Mizuno, Motoi Kanagawa, Kazuhiro Kobayashi, Tatsushi Toda, Tamao Endo
α-Dystroglycan (α-DG) is a highly glycosylated cell surface laminin receptor. Defects in the O -mannosyl glycan of an α-DG with laminin-binding activity can cause α-dystroglycanopathy, a group of congenital muscular dystrophies. In the biosynthetic pathway of functional O -mannosyl glycan, fukutin (FKTN) and fukutin-related protein (FKRP), whose mutated genes underlie α-dystroglycanopathy, sequentially transfer ribitol phosphate (RboP) from CDP-Rbo to form a tandem RboP unit (RboP-RboP) required for the synthesis of the laminin-binding epitope on O -mannosyl glycan...
June 8, 2018: Journal of Biological Chemistry
Christine Hellwig, Marta Barenys, Jenny Baumann, Kathrin Gassmann, Laura Casanellas, Gerhard Kauer, Ellen Fritsche
Human neural progenitor cells cultured as neurospheres are a promising tool for developmental neurotoxicity testing in vitro. In order to obtain a human cell-based tissue culture system as close to the organ as possible, it is desirable to improve the spatial organization of the "Neurosphere Assay" and use 3D scaffolds to better mimic the in vivo three dimensional cell microenvironment. For this reason we have established the conditions for short-term culture (up to 6 days) in matrigel or in IKVAV-3 peptide-functionalized hydrogels, and for long-term culture (>25 days) in IKVAV-3 peptide-functionalized hydrogels showing that these conditions support human neural progenitor cells' migration, differentiation to neurons and formation of neuronal networks...
June 5, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Justyna Borowiec, Joerg Hampl, Sukhdeep Singh, Sebastian Haefner, Karin Friedel, Patrick Mai, Dana Brauer, Florian Ruther, Liliana Liverani, Aldo Roberto Boccaccini, Andreas Schober
Micrometer-scale biochemical or topographical patterning is commonly used to guide the cell attachment and growth, but the ability to combine these patterns into an integrated surface with defined chemical and geometrical characteristics still remains a technical challenge. Here, we present a technical solution for simultaneous construction of 3D morphologies, in the form of channels, on porous membranes along with precise transfer of extracellular matrix proteins into the channels to create patterns with geometrically restricting features...
June 8, 2018: ACS Applied Materials & Interfaces
Olivier Gaudin, Vannina Seta, Marina Alexandre, Gérôme Bohelay, Françoise Aucouturier, Sabine Mignot-Grootenboer, Saskia Ingen-Housz-Oro, Céline Bernardeschi, Pierre Schneider, Benoît Mellottee, Frédéric Caux, Catherine Prost-Squarcioni
Mucous membrane pemphigoids (MMPs) and bullous pemphigoid (BP) are autoimmune bullous diseases that share physiopathological features: both can result from autoantibodies directed against BP180 or BP230 antigens. An association has been reported between BP and intake of gliptins, which are dipeptidyl peptidase-IV inhibitors used to treat type 2 diabetes mellitus. Clinical and immunological differences have been reported between gliptin-induced BPs and classical BPs: mucosal involvement, non-inflammatory lesions, and target BP180 epitopes other than the NC16A domain...
2018: Frontiers in Immunology
R Kong, N Wang, H Luo, J Lu
Hesperetin, a natural component of citrus fruits, is indicated to have beneficial anti-inflammatory effects on injury and various cancers as a transforming growth factor beta (TGF-β) inhibitor. However, little evidence associates hesperetin with liver fibrosis. Bile duct ligation (BDL) has been identified as a classic method of inducing liver fibrosis. An improvement in the bile duct operation is performed in this study. Work from our laboratory aims at finding the mechanism by which hesperetin attenuates liver fibrosis...
June 7, 2018: Current Molecular Medicine
Elena A Silantyeva, Wafaa Nasir, Jacqueline Carpenter, Olivia Manahan, Matthew L Becker, Rebecca K Willits
Substrates for embryonic stem cell culture are typified by poorly defined xenogenic, whole proteins or cellular components that are difficult and expensive to generate, characterize, and recapitulate. Herein, the generation of well-defined scaffolds of Gly-Tyr-Ile-Gly-Ser-Arg (GYIGSR) peptide-functionalized poly(ε-caprolactone) (PCL) aligned nanofibers are used to accelerate the neural lineage commitment and differentiation of D3 mouse embryonic stem cells (mESCs). Gene expression trends and immunocytochemistry analysis were similar to laminin-coated glass, and indicated an earlier differentiation progression than D3 mESCs on laminin...
June 4, 2018: Acta Biomaterialia
Abbass Haydar, Seav-Ly Tran, Elisabeth Guillemet, Claire Darrigo, Stéphane Perchat, Didier Lereclus, Laurent Coquet, Thierry Jouenne, Nalini Ramarao
Bacillus cereus is a Gram-positive spore-forming bacterium causing food poisoning and serious opportunistic infections. These infections are characterized by bacterial accumulation in the host despite the induction of inflammation. To circumvent inflammation, bacteria must resist the bactericidal activity of professional phagocytes, which constitute a first line of host defense against pathogens. Interactions between phagocytic cells and B. cereus are still poorly characterized and the mechanism of resistance to the host immune system is not known yet...
2018: Frontiers in Microbiology
Pedro M Rodríguez Cruz, Jacqueline Palace, David Beeson
Congenital myasthenic syndromes (CMS) are genetic disorders characterised by impaired neuromuscular transmission. This review provides an overview on CMS and highlights recent advances in the field, including novel CMS causative genes and improved therapeutic strategies. CMS due to mutations in SLC5A7 and SLC18A3 , impairing the synthesis and recycling of acetylcholine, have recently been described. In addition, a novel group of CMS due to mutations in SNAP25B , SYT2 , VAMP1 , and UNC13A1 encoding molecules implicated in synaptic vesicles exocytosis has been characterised...
June 5, 2018: International Journal of Molecular Sciences
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