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https://www.readbyqxmd.com/read/29678629/biological-activities-of-laminin-111-derived-peptide-chitosan-matrices-in-a-primary-culture-of-rat-cortical-neurons
#1
Hideki Hayashi, Mariko Yamada, Jun Kumai, Norio Takagi, Motoyoshi Nomizu
Cell adhesive biomaterials have been used for various cells in culture, especially for primary cultures of neurons. Here we examined laminin-111 and its active peptides conjugated to chitosan matrices (ChtMs) for primary culture of rat cortical neurons. Laminin-111 on poly-d-lysine substrate promoted neuronal cell attachment and differentiation. The biological activity of six active laminin-111-derived peptides was examined using a peptide-ChtM construct. When the syndecan-binding peptides, AG73 (RKRLQVQLSIRT, mouse laminin α1 chain 2719-2730) and C16 (KAFDITYVRLKF, laminin γ1 chain 139-150), were conjugated to chitosan, AG73-ChtM and C16-ChtM showed potent neuronal cell attachment activity and promoted axon extension by primary cultured rat cortical neurons...
April 17, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29677186/morphophysiological-changes-in-the-splenic-extracellular-matrix-of-leishmania-infantum-naturally-infected-dogs-is-associated-with-alterations-in-lymphoid-niches-and-the-cd4-t-cell-frequency-in-spleens
#2
Aurea Virginia Andrade da Silva, Fabiano Borges Figueiredo, Rodrigo Caldas Menezes, Arthur Augusto Mendes-Junior, Luisa Helena Monteiro de Miranda, Elisa Cupolillo, Renato Porrozzi, Fernanda Nazaré Morgado
The spleen is one of the main affected organs in canine visceral leishmaniasis (CVL). Disorganization of the splenic white pulp (SWP) has been associated with immunosuppression and disease progression. This study aims to assess structural and cellular changes in the splenic extracellular matrix of dogs with CVL, correlating these changes with the parasite load and clinical signs. Splenic fragments were collected from 41 naturally infected animals for parasite load quantification by quantitative PCR, histopathological analysis and immunohistochemistry for CD3+, CD4+, and CD8+ T cells; CD21+ B cells; Ki-67+, IFN-γ+, and IL-10+ cells; and the MMP-9 and ADAM-10 enzymes...
April 20, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29675520/designing-well-defined-photopolymerized-synthetic-matrices-for-three-dimensional-culture-and-differentiation-of-induced-pluripotent-stem-cells
#3
Elisa M Ovadia, David W Colby, April M Kloxin
Induced pluripotent stem cells (iPSCs) are of interest for the study of disease, where these cells can be derived from patients and have the potential to be differentiated into any cell type; however, three-dimensional (3D) culture and differentiation of iPSCs within well-defined synthetic matrices for these applications remains limited. Here, we aimed to establish synthetic cell-degradable hydrogels that allow precise presentation of specific biochemical cues for 3D culture of iPSCs with relevance for hypothesis testing and lineage-specific differentiation...
April 20, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29673759/alport-syndrome-and-pierson-syndrome-diseases-of-the-glomerular-basement-membrane
#4
REVIEW
Steven D Funk, Meei-Hua Lin, Jeffrey H Miner
The glomerular basement membrane (GBM) is an important component of the kidney's glomerular filtration barrier. Like all basement membranes, the GBM contains type IV collagen, laminin, nidogen, and heparan sulfate proteoglycan. It is flanked by the podocytes and glomerular endothelial cells that both synthesize it and adhere to it. Mutations that affect the GBM's collagen α3α4α5(IV) components cause Alport syndrome (kidney disease with variable ear and eye defects) and its variants, including thin basement membrane nephropathy...
April 16, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29667279/baicalein-disturbs-the-morphological-plasticity-and-motility-of-breast-adenocarcinoma-cells-depending-on-the-tumor-microenvironment
#5
Takeshi Terabayashi, Katsuhiro Hanada, Kou Motani, Hidetaka Kosako, Mami Yamaoka, Toshihide Kimura, Toshimasa Ishizaki
During tumor invasion, cancer cells change their morphology and mode of migration based on communication with the surrounding environment. Numerous studies have indicated that paracrine interactions from non-neoplastic cells impact the migratory and invasive properties of cancer cells. Thus, these interactions are potential targets for anticancer therapies. In this study, we showed that the flavones member baicalein suppresses the motility of breast cancer cells that is promoted by paracrine interactions. First, we identified laminin-332 (LN-332) as a principle paracrine factor in conditioned medium from mammary epithelium-derived MCF10A cells that regulates the morphology and motility of breast adenocarcinoma MDA-MB-231 cells...
April 18, 2018: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
https://www.readbyqxmd.com/read/29664206/pedf-vegf-ratio-plays-a-crucial-role-in-the-spontaneous-regression-of-infant-hemangioma-and-the-therapeutic-effect-of-propranolol
#6
Liuqing Zhu, Jinye Xie, Zhenyin Liu, Zhijian Huang, Mao Huang, Haofan Yin, Weiwei Qi, Zhonghan Yang, Ti Zhou, Guoquan Gao, Jing Zhang, Xia Yang
Infantile hemangioma (IH) is a benign tumor that is formed by aberrant angiogenesis and that undergoes spontaneous regression over time. Propranolol, the first-line therapy for IH, inhibits angiogenesis by downregulating the activation of the VEGF pathway, which is hyperactivated in IH. However, this treatment is reportedly ineffective for 10% of tumors, and 19% of patients relapse after propranolol treatment. Both pro-angiogenic and anti-angiogenic factors regulate angiogenesis, and PEDF is the most effective endogenous anti-angiogenic factor...
April 17, 2018: Cancer Science
https://www.readbyqxmd.com/read/29663071/cyclosporine-a-responsive-congenital-nephrotic-syndrome-with-single-heterozygous-variants-in-nphs1-nphs2-and-plce1
#7
Anna Eichinger, Sabine Ponsel, Carsten Bergmann, Roman Günthner, Julia Hoefele, Kerstin Amann, Bärbel Lange-Sperandio
BACKGROUND: Congenital nephrotic syndrome (CNS) is primarily a monogenetic disease, with the majority of cases due to changes in five different genes: the nephrin (NPHS1), podocin (NPHS2), Wilms tumor 1 (WT1), laminin ß2 (LAMB2), and phospholipase C epsilon 1 (PLCE1, NPHS3) gene. Usually CNS is not responsive to immunosuppressive therapy, but treatment with ACE inhibitors, AT1 receptor blockade and/or indomethacin can reduce proteinuria. If the disease progresses to end-stage renal disease, kidney transplantation is the therapy of choice...
April 16, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/29660511/comparative-adsorption-profiles-of-basal-lamina-proteome-and-gingival-cells-onto-dental-and-titanium-surfaces
#8
Mohamed-Nur Abdallah, Ghada Abughanam, Simon D Tran, Zeeshan Sheikh, Mohamed A Mezour, Tayebah Basiri, Yizhi Xiao, Marta Cerruti, Walter L Siqueira, Faleh Tamimi
Titanium (Ti) dental implants are susceptible to bacterial infections and failure due to lack of proper epithelial seal. Epithelial cells establish a strong epithelial seal around natural teeth by the deposition of basal lamina (BL) proteins that adsorb on the tooth surface. This seal can even be re-established onto cementum or dentin following injury or periodontal therapy. However, it is unclear how tooth surfaces promote this cell attachment and protein adsorption. Understanding the interactions between BL proteins and epithelial cells with dentin and Ti will facilitate the development of implant surfaces that promote the formation of an epithelial seal and improve the success of periodontal therapy and wound healing on natural teeth...
April 13, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29660229/extracellular-matrix-composition-modulates-pdac-parenchymal-and-stem-cell-plasticity-and-behavior-through-the-secretome
#9
Giulia Biondani, Katrine Zeeberg, Maria Raffaella Greco, Stefania Cannone, Ilaria Dando, Elisa Dalla Pozza, Maria Mastrodonato, Stefania Forciniti, Valeria Casavola, Marta Palmieri, Stephan Joel Reshkin, Rosa Angela Cardone
Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal cancers. Its aggressiveness is driven by an intense fibrotic desmoplastic reaction in which the increasingly collagen I-rich extracellular matrix (ECM) and several cell types, including cancer stem cells (CSCs), create a tumor-supportive environment. However, how ECM composition regulates CSC dynamics and their relationship to the principal parenchymal tumor population to promote early invasive growth is not yet characterized. For this we utilized a platform of 3D organotypic cultures composed of laminin-rich Matrigel, representative of an early tumor, plus increasing concentrations of collagen I to simulate malignant stroma progression...
April 16, 2018: FEBS Journal
https://www.readbyqxmd.com/read/29657251/laminin-1-acts-as-an-adhesive-for-odontoblast-like-cells-and-promotes-their-differentiation-toward-a-hard-tissue-forming-phenotype
#10
Jia Tang, Takashi Saito
The present study was designed to investigate the effect of laminin-1 (LN-1 or LN-111) on an odontoblast-like cell line, MDPC-23. Wells of non-treated polystyrene plates were coated with various concentrations of LN-1 (0.1, 1, 10, and 100 µg/mL) and left to dry for 2 days. Water-coated surfaces were used as controls. MDPC-23 cell proliferation, differentiation and mineralization were evaluated in terms of the CCK-8 assay, ALP activity, real-time RT-PCR and Alizarin red staining. The data indicated that LN-1 promoted the proliferation of MDPC-23 cells in a concentration-dependent manner...
April 16, 2018: Journal of Oral Science
https://www.readbyqxmd.com/read/29656608/combined-therapy-for-critical-limb-ischemia-biomimetic-plga-microcarriers-potentiates-the-pro-angiogenic-effect-of-adipose-tissue-stromal-vascular-fraction-cells
#11
Laurence Hoareau, Florian Fouchet, Cynthia Planesse, Sophie Mirbeau, Laurence Sindji, Emmanuel Delay, Régis Roche, Claudia N Montero-Menei, Franck Festy
We propose a regenerative solution in the treatment of critical limb ischemia. Poly-lactic/glycolic acid (PLGA) microcarriers were prepared and coated with laminin to be sterilized through γ-irradiation of 25 kGy at low temperature. Stromal vascular fraction (SVF) cells were extracted through enzymatic digestion of adipose tissue. Streptozotocin-induced diabetic mice underwent arteriotomy and received an administration of SVF cells combined or not with biomimetic microcarriers. Functional evaluation of the ischemic limb was then reported and tissue reperfusion was evaluated through fluorescence molecular tomography (FMT)...
April 14, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29653110/sirna-mediated-lrp-lr-knock-down-reduces-cellular-viability-of-malignant-melanoma-cells-through-the-activation-of-apoptotic-caspases
#12
Thalia M Rebelo, Leila Vania, Eloise Ferreira, Stefan F T Weiss
The 37kDa/67kDa laminin receptor (LRP/LR) is over-expressed in tumour cells and has been implicated in several tumourigenic processes such as metastasis and telomerase activation, however, more importantly the focus of the present study is on the maintenance of cellular viability and the evasion of apoptosis. The aim of the study was to investigate the role of LRP/LR on the cellular viability of early (A375) and late stage (A375SM) malignant melanoma cells. Flow cytometry and western blot analysis revealed that A375SM cells contain more cell-surface and total LRP/LR levels in comparison to the A375 cells, respectively...
April 10, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/29652132/trimetallic-hybrid-nanoflower-decorated-mos2-nanosheet-sensor-for-direct-in-situ-monitoring-of-h2o2-secreted-from-live-cancer-cells
#13
Baoting Dou, Jianmei Yang, Ruo Yuan, Yun Xiang
In situ monitoring of hydrogen peroxide (H2O2) secreted from live cells plays critical roles in elucidating many cellular signaling pathways, and it is of significant challenge to selectively detect these low levels of endogenous H2O2. To address this challenge, we report the establishment of a trimetallic hybrid nanoflower-decorated MoS2 nanosheet-modified sensor for in situ monitoring of H2O2 secreted from live MCF-7 cancer cells. The Au-Pd-Pt nanoflower-dispersed MoS2 nanosheets are synthesized by a simple wet-chemistry method, and the resulting nanosheet composites exhibit significantly enhanced catalytic activity toward electrochemical reduction of H2O2, due to the synergistic effect of the highly dispersed trimetallic hybrid nanoflowers and the MoS2 nanosheets, thereby resulting in ultra-sensitive detection of H2O2 with a sub-nanomolar level detection limit in vitro...
April 13, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29652027/pokeweed-antiviral-protein-attenuates-liver-fibrosis-in-mice-through-regulating-wnt-jnk-mediated-glucose-metabolism
#14
Yonghong Zhang, Xiaolan Yu, Zheng Wang, Ming Yin, Zonghao Zhao, Yi Li, Wenting Li
Background/Aims: Pokeweed antiviral protein (PAP) has been reported to downregulate Wnt/Jnk pathway and attenuate liver fibrosis. This study was designed to intensively explore the mechanism of anti-fibrosis effect of PAP. Materials and Methods: Hepatic stellate cell (HSC) activation was induced by high concentration of glucose. Cell viability was detected at different time points after PAP treatment. Meanwhile, hepatic fibrosis models in mice were induced by CCl4 injection...
April 13, 2018: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/29651375/codon-optimization-significantly-enhanced-the-expression-of-human-37-kda-ilrp-in-escherichia-coli
#15
Bainan Liu, Qianqian Kong, Dong Zhang, Lingli Yan
37-kDa immature laminin receptor protein (iLRP), the precursor of 67-kDa laminin receptor protein (LRP), is overexpressed on the surface of most cancer cells and recognized as a universal tumor antigen. The role makes it a potential target for cancer immunotherapy, which has been well-studied. Our study aimed to produce high quality of human iLRP in bacteria so that the needs in research of its clinical application could be met. The powerful system for heterologous protein expression, pET system was used. Two types of DNA sequences encoding the same amino acid sequences were separately cloned into the vector pET30a(+)...
April 2018: 3 Biotech
https://www.readbyqxmd.com/read/29642582/synthesis-of-a-novel-electrospun-polycaprolactone-scaffold-functionalized-with-ibuprofen-for-periodontal-regeneration-an-in-vitro-andin-vivo-study
#16
Fareeha Batool, David-Nicolas Morand, Lionel Thomas, Isaac Maximiliano Bugueno, Javier Aragon, Silvia Irusta, Laetitia Keller, Nadia Benkirane-Jessel, Henri Tenenbaum, Olivier Huck
Ibuprofen (IBU) has been shown to improve periodontal treatment outcomes. The aim of this study was to develop a new anti-inflammatory scaffold by functionalizing an electrospun nanofibrous poly-ε-caprolactone membrane with IBU (IBU-PCL) and to evaluate its impact on periodontal inflammation, wound healing and regeneration in vitro and in vivo. IBU-PCL was synthesized through electrospinning. The effects of IBU-PCL on the proliferation and migration of epithelial cells (EC) and fibroblasts (FB) exposed to Porphyromonas gingivlais lipopolysaccharide (Pg -LPS) were evaluated through the AlamarBlue test and scratch assay, respectively...
April 10, 2018: Materials
https://www.readbyqxmd.com/read/29627521/epidermolysis-bullosa-molecular-pathology-of-connective-tissue-components-in-the-cutaneous-basement-membrane-zone
#17
REVIEW
Cristina Has, Alexander Nyström, Amir Hossain Saeidian, Leena Bruckner-Tuderman, Jouni Uitto
Epidermolysis bullosa (EB), a group of heritable skin fragility disorders, is characterized by blistering, erosions and chronic ulcers in the skin and mucous membranes. In some forms, the blistering phenotype is associated with extensive mutilating scarring and development of aggressive squamous cell carcinomas. The skin findings can be associated with extracutaneous manifestations in the ocular as well as gastrointestinal and vesico-urinary tracts. The phenotypic heterogeneity reflects the presence of mutations in as many as 20 different genes expressed in the cutaneous basement membrane zone, and the types and combinations of the mutations and their consequences at the mRNA and protein levels contribute to the spectrum of severity encountered in different subtypes of EB...
April 5, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/29625765/lema-and-erp-y-like-recombinant-proteins-from-leptospira-interrogans-protect-hamsters-from-challenge-using-addavax%C3%A2-as-adjuvant
#18
Thaís Larré Oliveira, Rodrigo Andrade Schuch, Guilherme Roig Inda, Bárbara Couto Roloff, Amilton Clair Pinto Seixas Neto, Marta Amaral, Odir Antonio Dellagostin, Daiane Drawanz Hartwig
BACKGROUND: Recombinant subunit vaccines have been extensively evaluated as promising alternatives against leptospirosis. Here, we evaluated two proteins in formulations containing the adjuvant AddaVax™ as vaccine candidates for prevention and control of leptospirosis. METHODS: Recombinant proteins rErp Y-like and rLemA were characterized by ELISA to assess their ability to bind extracellular matrix (ECM) components and fibrinogen. Groups of eight hamsters were immunized intramuscularly with rErp Y-like or rLemA mixed with a squalene-based adjuvant (AddaVax), and then vaccine efficacy was determined in terms of protection against a lethal challenge...
April 3, 2018: Vaccine
https://www.readbyqxmd.com/read/29623298/a-murine-model-of-charcot-marie-tooth-disease-4f-reveals-a-role-for-the-c-terminus-of-periaxin-in-the-formation-and-stabilization-of-cajal-bands
#19
Diane L Sherman, Peter J Brophy
Charcot-Marie-Tooth (CMT) disease comprises up to 80 monogenic inherited neuropathies of the peripheral nervous system (PNS) that collectively result in demyelination and axon degeneration. The majority of CMT disease is primarily either dysmyelinating or demyelinating in which mutations affect the ability of Schwann cells to either assemble or stabilize peripheral nerve myelin. CMT4F is a recessive demyelinating form of the disease caused by mutations in the Periaxin ( PRX ) gene . Periaxin (Prx) interacts with Dystrophin Related Protein 2 (Drp2) in an adhesion complex with the laminin receptor Dystroglycan (Dag)...
2018: Wellcome Open Research
https://www.readbyqxmd.com/read/29621176/functionalized-thick-film-impedance-sensors-for-use-in-in-vitro-cell-culture
#20
Heike Bartsch, Martin Baca, Uta Fernekorn, Jens Müller, Andreas Schober, Hartmut Witte
Multi-electrode arrays find application in electrophysiological recordings. The quality of the captured signals depends on the interfacial contact between electrogenic cells and the electronic system. Therefore, it requires reliable low-impedance electrodes. Low-temperature cofired ceramic technology offers a suitable platform for rapid prototyping of biological reactors and can provide both stable fluid supply and integrated bio-hardware interfaces for recordings in electrogenic cell cultures. The 3D assembly of thick film gold electrodes in in vitro bio-reactors has been demonstrated for neuronal recordings...
April 5, 2018: Biosensors
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