keyword
MENU ▼
Read by QxMD icon Read
search

Laminin

keyword
https://www.readbyqxmd.com/read/28208029/l1-peptide-conjugated-fibrin-hydrogels-promote-salivary-gland-regeneration
#1
K Nam, C-S Wang, C L M Maruyama, P Lei, S T Andreadis, O J Baker
Hyposalivation contributes to dental caries, periodontitis, and microbial infections. Additionally, it impairs activities of daily living (e.g., speaking, chewing, and swallowing). Treatments for hyposalivation are currently limited to medications (e.g., the muscarinic receptor agonists pilocarpine and cevimeline) that induce saliva secretion from residual acinar cells and the use of saliva substitutes. However, given that these therapies provide only temporary relief, the development of alternative treatments to restore gland function is essential...
February 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28205281/growth-hormone-modulates-in-vitro-endothelial-cell-migration-and-formation-of-capillary-like-structures
#2
Cícero Fagner Messias de Lima, Maria Danielma Dos Santos Reis, Fernando Wagner da Silva Ramos, Silvana Ayres-Martins, Salete Smaniotto
The generation of new blood vessels is a complex process mediated by a variety of growth factors, and the growth hormone (GH) has been shown to act as a proangiogenic factor. In fact, human GH deficiency or excess are associated with endothelial dysfunction. Moreover, mouse models have revealed the action of GH in both tissue repair and in the microvascular circulation of normal tissues. In this study, we investigated the in vitro effects of GH on endothelial cells. Using a murine endothelioma cell line (tEnd...
February 16, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28198346/characterization-of-two-new-putative-adhesins-of-leptospira-interrogans
#3
Jupciana M Figueredo, Gabriela H Siqueira, Gisele O de Souza, Marcos B Heinemann, Silvio A Vasconcellos, Erica G B Chapola, Ana L T O Nascimento
We here report the characterization of two novel proteins encoded by the genes LIC11122 and LIC12287, identified in the genome sequences of Leptospira interrogans, annotated, respectively, as a putative sigma factor and a hypothetical protein. The CDSs LIC11122 and LIC12287 have signal peptide SPII and SPI and are predicted to be located mainly at the cytoplasmic membrane of the bacteria. The genes were cloned and the proteins expressed using Escherichia coli. Proteinase K digestion showed that both proteins are surface exposed...
January 2017: Microbiology
https://www.readbyqxmd.com/read/28196225/blood-vessel-basement-membrane-alterations-in-human-retinal-microaneurysms-during-aging
#4
Mariana López-Luppo, Victor Nacher, David Ramos, Joana Catita, Marc Navarro, Ana Carretero, Alfonso Rodriguez-Baeza, Luísa Mendes-Jorge, Jesús Ruberte
Purpose: Microaneurysms, considered a hallmark of retinal vascular disease, are present in aged retinas. Here, the basement membrane of human retinal microaneurysms has been analyzed during aging. Methods: Retinas were obtained from 17 nondiabetic donors. Whole mount retinas and paraffin sections were marked immunohistochemically with antibodies against the main components of the blood basement membrane. Trypsin digestion and transmission electron microscopy also were performed...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28192799/cyp1b1-regulates-ocular-fissure-closure-through-a-retinoic-acid-independent-pathway
#5
Antionette L Williams, Jessica Eason, Bahaar Chawla, Brenda L Bohnsack
Purpose: Mutations in the CYP1B1 gene are the most commonly identified genetic causes of primary infantile-onset glaucoma. Despite this disease association, the role of CYP1B1 in eye development and its in vivo substrate remain unknown. In the present study, we used zebrafish to elucidate the mechanism by which cyp1b1 regulates eye development. Methods: Zebrafish eye and neural crest development were analyzed using live imaging of transgenic zebrafish embryos, in situ hybridization, immunostaining, TUNEL assay, and methylacrylate sections...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28188379/a-novel-mutation-of-laminin-%C3%AE-2-lamb2-in-two-siblings-with-renal-failure
#6
Farah A Falix, Carlien A M Bennebroek, Bert van der Zwaag, Ruth Lapid-Gortzak, Sandrine Florquin, Michiel J S Oosterveld
: This report describes a novel mutation of LAMB2, the gene associated with Pierson syndrome (microcoria-congenital nephrosis syndrome), in two female siblings. The c.970T>C p.(Cys324Arg) mutation in the LAMB2 gene affects one of the eight highly conserved cysteine residues within the first EGF-like module of the laminin β2 protein. These residues form disulfide bonds in order to achieve a correct 3D structure of the protein. The reported phenotype is considered a relatively mild variant of Pierson syndrome and is associated with later-onset (18 months) therapy-resistant nephrotic syndrome leading to renal failure, and ocular abnormalities consisting of high myopia, microcoria, diverse retinal abnormalities, hence a low level of visual acuity...
February 10, 2017: European Journal of Pediatrics
https://www.readbyqxmd.com/read/28188286/hydrogen-sulfide-inhibits-high-glucose-induced-nadph-oxidase-4-expression-and-matrix-increment-by-recruiting-inducible-nitric-oxide-synthase-in-the-kidney-proximal-tubular-epithelial-cells
#7
Hak Joo Lee, Doug Yoon Lee, Meenalakshmi M Mariappan, Denis Feliers, Goutam Ghosh Choudhury, Hanna E Abboud, Yves Gorin, Balakuntalam S Kasinath
High glucose increases NADPH oxidase 4 (NOX4) expression, reactive oxygen species (ROS) generation, and, matrix protein synthesis by inhibiting AMP-activated protein kinase (AMPK) in renal cells. Since hydrogen sulfide (H2S) inhibits high glucose-induced matrix protein increment by activating AMPK in renal cells, we examined if H2S inhibits high glucose induced expression of NOX4 and matrix protein and if H2S nitric oxide (NO) pathways are integrated. High glucose increased NOX4 expression and activity at 24 h in renal proximal tubular epithelial cells that was inhibited by sodium hydrosulfide (NaHS), a source of H2S...
February 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28187500/basement-membrane-type-iv-collagen-and-laminin-an-overview-of-their-biology-and-value-as-fibrosis-biomarkers-of-liver-disease
#8
REVIEW
Ki M Mak, Rena Mei
Basement membranes provide structural support to epithelium, endothelium, muscles, fat cells, Schwann cells and axons. Basement membranes are multifunctional: they modulate cellular behavior, regulate organogenesis, promote tissue repair, form a barrier to filtration and tumor metastasis, bind growth factors and mediate angiogenesis. All basement membranes contain type IV collagen (Col IV), laminin, nidogen, and perlecan. Col IV and laminin self-assemble into two independent supramolecular networks that are linked to nidogen and perlecan to form a morphological discernable basement membrane/basal lamina...
February 10, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28182100/semaphorin-3a-increases-fak-phosphorylation-at-focal-adhesions-to-modulate-mda-mb-231-cell-migration-and-spreading-on-different-substratum-concentrations
#9
Scott Gehler, Frances V Compere, Alex M Miller
Interactions between integrin-mediated adhesions and the extracellular matrix (ECM) are important regulators of cell migration and spreading. However, mechanisms by which extracellular ligands regulate cell migration and spreading in response to changes in substratum concentration are not well understood. Semaphorin 3A (Sema3A) has been shown to inhibit cell motility and alter integrin signaling in various cell types. We propose that Sema3A alters focal adhesions to modulate breast carcinoma cell migration and spreading on substrata coated with different concentrations of ECM...
2017: International Journal of Breast Cancer
https://www.readbyqxmd.com/read/28179297/cilengitide-and-cetuximab-reduce-cytokine-production-and-colony-formation-of-head-and-neck-squamous-cell-carcinoma-cells-ex-vivo
#10
Gunnar Wichmann, Susan Cedra, Daphne Schlegel, Marlen Kolb, Susanne Wiegand, Andreas Boehm, Mathias Hofer, Andreas Dietz
BACKGROUND/AIM: To analyze ex vivo effects of combined targeting of the epidermal growth factor-receptor (EGFR) by cetuximab (E) plus αVβ3 and αVβ5 integrins by cilengitide (Cil) on colony formation of epithelial cells (CFec) and release of pro-angiogenetic and pro-inflammatory cytokines in head and neck squamous cell carcinoma (HNSCC) cells. MATERIALS AND METHODS: Collagenase-IV digests of 43 histopathological confirmed HNSCC cases were seeded into laminin-coated 96-well plates containing E, Cil, or Cil+E in final concentrations of 66...
2017: Anticancer Research
https://www.readbyqxmd.com/read/28174005/the-expression-of-cancer-stem-cell-markers-in-human-colorectal-carcinoma-cells-in-a-microenvironment-dependent-manner
#11
Vaidotas Stankevicius, Linas Kunigenas, Edvinas Stankunas, Karolina Kuodyte, Egle Strainiene, Jonas Cicenas, Narimantas E Samalavicius, Kestutis Suziedelis
Numerous lines of evidence support the hierarchical model of cancer development and tumor initiation. According to the theory, cancer stem cells play a crucial role in the formation of the tumor and should be targeted for more effective anticancer treatment. However, cancer stem cells quickly loose their characteristics when propagated as 2D cell culture, indicating that the 2D cell culture does not provide the appropriate settings to maintain an in vivo environment. In this study we have investigated the expression of self-renewal, cancer stem cell and epithelial to mesenchymal transition markers after the transfer of human colorectal carcinoma cell DLD1 and HT29 lines from 2D cell cultures to scaffold-attached laminin rich extracellular matrix and scaffold-free multicellular spheroid 3D culture models...
February 4, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28173861/laminin-differentially-regulates-the-stemness-of-type-i-and-type-ii-pericytes
#12
Jyoti Gautam, Abhijit Nirwane, Yao Yao
BACKGROUND: Laminin, a major basement membrane component that has direct contact with pericytes under physiological conditions, actively regulates the proliferation and differentiation/fate determination of pericytes. Recently, two types of pericytes (type I and type II) with different molecular markers and functions have been identified in skeletal muscles. Whether laminin differentially regulates the proliferation and differentiation of these two subpopulations remains unclear. METHODS: Wild-type and pericytic laminin-deficient mice under Nestin-GFP background were used to determine if laminin differentially regulates the proliferation and differentiation of type I and type II pericytes...
February 7, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28167671/defining-the-ail-ligand-binding-surface-hydrophobic-residues-in-two-extracellular-loops-mediate-cell-and-extracellular-matrix-binding-to-facilitate-yop-delivery
#13
Tiffany M Tsang, Jeffrey S Wiese, Jamal A Alhabeil, Lisa D Usselman, Joshua J Thomson, Rafla Matti, Malte Kronshage, Natalie Maricic, Shanedah Williams, Naama H Sleiman, Suleyman Felek, Eric S Krukonis
Yersinia pestis, the causative agent of plague, binds host cells to deliver cytotoxic Yop proteins into the cytoplasm that prevent phagocytosis and generation of pro-inflammatory cytokines. Ail is an eight-stranded β-barrel outer membrane protein with four extracellular loops that mediates cell binding and resistance to human serum. Following deletion of each of the four extracellular loops that potentially interact with host cells, the Ail-Δloop 2 and Ail-Δloop 3 mutants had no cell binding activity while Ail-Δloop 4 maintained cell binding (the Ail-Δloop 1 protein was unstable)...
February 6, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28167232/integrative-network-analysis-reveals-time-dependent-molecular-events-underlying-left-ventricular-remodeling-in-post-myocardial-infarction-patients
#14
Florence Pinet, Marie Cuvelliez, Thomas Kelder, Philippe Amouyel, Marijana Radonjic, Christophe Bauters
To elucidate the time-resolved molecular events underlying the LV remodeling (LVR) process, we developed a large-scale network model that integrates the 24 molecular variables (plasma proteins and non-coding RNAs) collected in the REVE-2 study at four time points (baseline, 1month, 3months and 1year) after MI. The REVE-2 network model was built by extending the set of REVE-2 variables with their mechanistic context based on known molecular interactions (1310 nodes and 8639 edges). Changes in the molecular variables between the group of patients with high LVR (>20%) and low LVR (<20%) were used to identify active network modules within the clusters associated with progression of LVR, enabling assessment of time-resolved molecular changes...
February 3, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28165558/the-relationship-between-serum-visfatin-and-the-progress-of-chronic-viral-hepatitis-b-cirrhosis
#15
X-L Wei
OBJECTIVE: To explore the relationship between serum visfatin and the progress of chronic viral hepatitis B cirrhosis. PATIENTS AND METHODS: Selected 153 cases of chronic hepatitis B patients with cirrhosis as an observational group. Among which, Child-Pugh Grade was divided into grade A with 31 cases, grade B with 79 cases and grade C with 43 cases. 50 healthy people were added to the study and were considered as control group. Comparison of the sera levels of visfatin, blood lipid, liver fibrosis indexes, fasting plasma glucose and fasting insulin of the observational and those of the control group were done...
January 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28164151/graphene-foam-as-a-three-dimensional-platform-for-myotube-growth
#16
Eric Krueger, A Nicole Chang, Dale Brown, Josh Eixenberger, Raquel Brown, Sepideh Rastegar, Katie M Yocham, Kurtis D Cantley, David Estrada
This study demonstrates the growth and differentiation of C2C12 myoblasts into functional myotubes on 3-dimensional graphene foam bioscaffolds. Specifically, we establish both bare and laminin coated graphene foam as a biocompatible platform for muscle cells and identify that electrical coupling stimulates cell activity. Cell differentiation and functionality is determined by the expression of myotube heavy chain protein and Ca(2+) fluorescence, respectively. Further, our data show that the application of a pulsed electrical stimulus to the graphene foam initiates myotube contraction and subsequent localized substrate movement of over 100 micrometers...
August 8, 2016: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28164129/laminin-521-promotes-rat-bone-marrow-mesenchymal-stem-cell-sheet-formation-on-light-induced-cell-sheet-technology
#17
Zhiwei Jiang, Yue Xi, Kaichen Lai, Ying Wang, Huiming Wang, Guoli Yang
Rat bone marrow mesenchymal stem cell sheets (rBMSC sheets) are attractive for cell-based tissue engineering. However, methods of culturing rBMSC sheets are critically limited. In order to obtain intact rBMSC sheets, a light-induced cell sheet method was used in this study. TiO2 nanodot films were coated with (TL) or without (TN) laminin-521. We investigated the effects of laminin-521 on rBMSCs during cell sheet culturing. The fabricated rBMSC sheets were subsequently assessed to study cell sheet viability, reattachment ability, cell sheet thickness, collagen type I deposition, and multilineage potential...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28159878/laminins-in-epithelial-cell-polarization-old-questions-in-search-of-new-answers
#18
Karl S Matlin, Satu-Marja Myllymäki, Aki Manninen
Laminin, a basement membrane protein discovered in 1979, was shortly thereafter implicated in the polarization of epithelial cells in both mammals and a variety of lower organisms. To transduce a spatial cue to the intrinsic polarization machinery, laminin must polymerize into a dense network that forms the foundation of the basement membrane. Evidence suggests that activation of the small GTPase Rac1 by β1-integrins mobilizes laminin-binding integrins and dystroglycan to consolidate formation of the laminin network and initiate rearrangements of both the actin and microtubule cytoskeleton to help establish the apicobasal axis...
February 3, 2017: Cold Spring Harbor Perspectives in Biology
https://www.readbyqxmd.com/read/28156053/urothelial-cell-expansion-and-differentiation-are-improved-by-exposure-to-hypoxia
#19
Stéphane Chabaud, Ingrid Saba, Clément Baratange, Brice Boiroux, Maude Leclerc, Alexandre Rousseau, Sara Bouhout, Stéphane Bolduc
Cells obtained from a patient's biopsy have to be expanded after extraction to produce autologous tissues, but standard cell culture conditions often limit their growth or lifespan and could induce early and inadequate cell differentiation. Moreover, it has previously been reported that the air-liquid interface, that induces maturation of the urothelium, stimulated inadequate differentiation associated with aberrant keratin-14 expression. The aim of this study was to test the benefits of hypoxia during expansion of urothelial cells and maturation of the bladder epithelium in the context of tissue engineering...
February 3, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28154178/green-tea-polyphenol-epigallocatechin-3-gallate-suppresses-toll-like-receptor-4-expression-via-upregulation-of-e3-ubiquitin-protein-ligase-rnf216
#20
Motofumi Kumazoe, Yuki Nakamura, Mai Yamashita, Takashi Suzuki, Kanako Takamatsu, Yuhui Huang, Jaehoon Bae, Shuya Yamashita, Motoki Murata, Shuhei Yamada, Yuki Shinoda, Wataru Yamaguchi, Yui Toyoda, Hirofumi Tachibana
Toll-like receptor 4 (TLR4) plays an essential role in innate immunity through inflammatory cytokine induction. Recent studies demonstrated that the abnormal activation of TLR4 has a pivotal role in obesity-induced inflammation, which is associated with several diseases, including hyperinsulinemia, hypertriglyceridemia, and cardiovascular disease. Here we demonstrate that (-)-epigallocatechin-3-O-gallate, a natural agonist of the 67-kDa laminin receptor (67LR), suppressed TLR4 expression through E3 ubiquitin-protein ring finger protein 216 (RNF216) upregulation...
February 1, 2017: Journal of Biological Chemistry
keyword
keyword
69128
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"