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https://www.readbyqxmd.com/read/27920471/identification-of-il11ra-and-melk-amplification-in-gastric-cancer-by-comprehensive-genomic-profiling-of-gastric-cancer-cell-lines
#1
Danielle Queiroz Calcagno, Sylvia Santomi Takeno, Carolina Oliveira Gigek, Mariana Ferreira Leal, Fernanda Wisnieski, Elizabeth Suchi Chen, Taíssa Maíra Thomaz Araújo, Eleonidas Moura Lima, Maria Isabel Melaragno, Samia Demachki, Paulo Pimentel Assumpção, Rommel Rodriguez Burbano, Marília Cardoso Smith
AIM: To identify common copy number alterations on gastric cancer cell lines. METHODS: Four gastric cancer cell lines (ACP02, ACP03, AGP01 and PG100) underwent chromosomal comparative genome hybridization and array comparative genome hybridization. We also confirmed the results by fluorescence in situ hybridization analysis using the bacterial artificial chromosome clone and quantitative real time PCR analysis. RESULTS: The amplification of 9p13...
November 21, 2016: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/27918868/handgrip-dynamometry-and-patient-generated-subjective-global-assessment-in-patients-with-nonresectable-lung-cancer
#2
Ana T Barata, Carla Santos, Marília Cravo, Maria do Céu Vinhas, Catarina Morais, Elisabete Carolino, Lino Mendes, Jorge Roldão Vieira, Jorge Fonseca
INTRODUCTION: Undernutrition is frequently associated with advanced lung cancer. Accurate nutritional assessment tools are important to provide the proper nutritional therapy. Handgrip dynamometry has already been used in these patients, and the findings suggest that it is a good indicator of nutritional status. AIMS: The aim of this study was to evaluate the association between nutritional status and handgrip strength (HGS) in patients with nonresectable lung cancer...
December 5, 2016: Nutrition and Cancer
https://www.readbyqxmd.com/read/27914221/prevalence-of-trypanosoma-cruzi-hiv-coinfection-in-southern-brazil
#3
Dulce Stauffert, Mariangela Freitas da Silveira, Marília Arndt Mesenburg, Adriane Brod Manta, Alessandra da Silva Dutra, Guilherme Lucas de Oliveira Bicca, Marcos Marreiro Villela
Chagas disease reactivation has been a defining condition for acquired immune deficiency syndrome in Brazil for individuals coinfected with Trypanosoma cruzi and HIV since 2004. Although the first coinfection case was reported in the 1980s, its prevalence has not been firmly established. In order to know coinfection prevalence, a cross-sectional study of 200 HIV patients was performed between January and July 2013 in the city of Pelotas, in southern Rio Grande do Sul, an endemic area for Chagas disease. Ten subjects were found positive for T...
November 30, 2016: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27911252/chapparvoviruses-occur-in-at-least-three-vertebrate-classes-and-have-a-broad-biogeographic-distribution
#4
William Marciel de Souza, Marilia Farignoli Romeiro, Marcílio Jorge Fumagalli, Sejal Modha, Jansen de Araujo, Luzia Helena Queiroz, Edison Luiz Durigon, Luiz Tadeu Moraes Figueiredo, Pablo Ramiro Murcia, Robert Gifford
Chapparvoviruses are a highly divergent group of parvoviruses (family Parvoviridae) that have recently been identified via metagenomic sampling of animal faeces. Here we report the sequences of six novel chapparvoviruses identified through both metagenomic sampling of bat tissues and in silico screening of published vertebrate genome assemblies. The novel chapparvoviruses share several distinctive genomic features, and group together as a robustly supported monophyletic clade in phylogenetic trees. Our data indicate that chapparvoviruses have a broad host range in vertebrates, and a global distribution...
December 2, 2016: Journal of General Virology
https://www.readbyqxmd.com/read/27908404/fluoride-affects-bone-repair-differently-in-mice-models-with-distinct-bone-densities
#5
Paula Kennerly Herrera, Andressa Pelissari Zambolin, Mileni da Silva Fernandes, Tânia Mary Cestari, Flávia Godoy Iano, Willian Fernando Zambuzzi, Marília Afonso Rabelo Buzalaf, Rodrigo Cardoso de Oliveira
We grouped mice [strains: C57BL/6J (n=32) and C3H/HeJ (n=32)] to address the influence of bone density on fluoride's (F's) biological effects. These animals received low-fluoride food and water containing 0 (control group) or 50ppm of F for up to 28days. The upper left central incisor was extracted, and the left maxilla was collected at 7, 14, 21, and 28days for histological and histomorphometric analysis to estimate bone neoformation. Our results showed bone neoformation in all of the evaluated groups, with the presence of bone islets invading the center of the alveoli when replacing the existing connective tissue...
January 2017: Journal of Trace Elements in Medicine and Biology
https://www.readbyqxmd.com/read/27901201/evaluation-of-a-preventive-educational-program-for-malocclusions-7-year-study
#6
Beatriz Brandão Scarpelli, Sandrine Bittencourt Berger, Marília Franco Punhagui, Cássio Alexandre Zeri de Oliveira, Antonio Ferelle, Paula Vanessa Pedron Oltramari-Navarro
Results: 506 of the 802 guardians/children referred to the PPM joined. As for the children, the most frequently assessed habits were: bottle (56.1%), bottle and pacifier (18.4%), finger (11.9%), bottle and finger (7.1%), pacifier (5.7%), pacifier and finger (0.6%), and bottle/pacifier/finger (0.2%). After parent participation in the meetings, 335 (66.2%) children abandoned their habits. There was a statistical difference between type of oral habit and time to abandonment (p = 0.0001)...
November 28, 2016: Brazilian Oral Research
https://www.readbyqxmd.com/read/27899335/design-and-characterization-of-plasmids-encoding-antigenic-peptides-of-aha1-from-aeromonas-hydrophila-as-prospective-fish-vaccines
#7
Pradipta R Rauta, Bismita Nayak, Gabriel A Monteiro, Marília Mateus
The current investigation aimed at designing DNA vaccines against Aeromonas hydrophila infections. The DNA vaccine candidates were designed to express two antigenic outer membrane protein (Aha1) peptides and to be delivered by a nanoparticle-based delivery system. Gene sequences of conserved regions of antigenic Aha1 [aha1(211-381), aha1(211-381)opt, aha1(703-999) and aha1(703-999)opt] were cloned into pVAX-GFP expression vector. The selected DNA vaccine candidates were purified from E. coli DH5α and transfected into Chinese hamster ovary cells...
November 26, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27898426/maternal-depression-increases-childhood-dental-caries-a-cohort-study-in-brazil
#8
Gabriela Dos Santos Pinto, Luciana de Ávila Quevedo, Marcos Britto Correa, Marina Sousa Azevedo, Marília Leão Goettems, Ricardo Tavares Pinheiro, Flávio Fernando Demarco
AIM: To investigate the relationship between maternal depression and childhood caries in a cohort of adolescent mothers. METHODS: This cross-sectional study nested in a cohort evaluated a sample of 538 mother/child dyads. When the children were 24-36 months of age, data regarding oral health from children and mothers were collected by clinical dental examination. A mother's major depressive disorder was assessed by using the Mini International Neuropsychiatric Interview (MINI [Plus]), at the current moment...
November 30, 2016: Caries Research
https://www.readbyqxmd.com/read/27882250/apical-revascularization-after-delayed-tooth-replantation-an-unusual-case
#9
Marília Pacífico Lucisano, Paulo Nelson-Filho, Lea Assed Bezerra Silva, Raquel Assed Bezerra Silva, Fabricio Kitazono de Carvalho, Alexandra Mussolino de Queiroz
The aim of this paper is to present the clinical and radiological outcome of the treatment involving a delayed tooth replantation after an avulsed immature permanent incisor, with a follow-up of 1 year and 6 months. An 8-year-old boy was referred after dental trauma that occurred on the previous day. The permanent maxillary right central incisor (tooth 11) had been avulsed. The tooth was hand-held during endodontic therapy and an intracanal medication application with calcium hydroxide-based paste was performed...
2016: Case Reports in Dentistry
https://www.readbyqxmd.com/read/27880005/s-nitrosoglutathione-promotes-cell-wall-remodelling-alters-the-transcriptional-profile-and-induces-root-hair-formation-in-the-hairless-root-hair-defective-6-rhd6-mutant-of-arabidopsis-thaliana
#10
Camila Fernandes Moro, Marilia Gaspar, Felipe Rodrigues da Silva, Sivakumar Pattathil, Michael G Hahn, Ione Salgado, Marcia Regina Braga
Nitric oxide (NO) exerts pleiotropic effects on plant development; however, its involvement in cell wall modification during root hair formation (RHF) has not yet been addressed. Here, mutants of Arabidopsis thaliana with altered root hair phenotypes were used to assess the involvement of S-nitrosoglutathione (GSNO), the primary NO source, in cell wall dynamics and gene expression in roots induced to form hairs. GSNO and auxin restored the root hair phenotype of the hairless root hair defective 6 (rhd6) mutant...
November 23, 2016: New Phytologist
https://www.readbyqxmd.com/read/27870316/a-comparative-review-of-gerontological-nursing-citation-data
#11
REVIEW
Marilia Y Antunez, Marcia Henry
BACKGROUND: Gerontological nursing is a highly interdisciplinary specialty. English-language bibliometric research is scarce. OBJECTIVES: Study gerontological nursing literature to identify journals useful for nurses and researchers working with older adults, help librarians assist gerontological nursing authors to find publication outlets and make library collection decisions. METHODS: Using a combination of methods, the authors identified cited journals in the Journal of Gerontological Nursing (JGN) and Geriatric Nursing (GN) from 2008 to 2010 using part of the Nursing and Allied Health Resources (NAHRS) Section protocol...
December 2016: Health Information and Libraries Journal
https://www.readbyqxmd.com/read/27865671/anomalous-pulmonary-venous-connection-an-underestimated-entity
#12
Sara P Magalhães, Nuno Moreno, Marília Loureiro, Manuela França, Fernanda Reis, Sílvia Alvares, Manuel Ribeiro
Anomalous pulmonary venous connection is an uncommon congenital anomaly in which all (total form) or some (partial form) pulmonary veins drain into a systemic vein or into the right atrium rather than into the left atrium. The authors present one case of total anomalous pulmonary venous connection and two cases of partial anomalous pulmonary venous connection, one of supracardiac drainage into the brachiocephalic vein, and the other of infracardiac anomalous venous drainage (scimitar syndrome). Through the presentation of these cases, this article aims to review the main pulmonary venous developmental defects, highlighting the role of imaging techniques in the assessment of these anomalies...
December 2016: Portuguese Journal of Cardiology: An Official Journal of the Portuguese Society of Cardiology
https://www.readbyqxmd.com/read/27863420/ywhae-silencing-induces-cell-proliferation-invasion-and-migration-through-the-up-regulation-of-cdc25b-and-myc-in-gastric-cancer-cells-new-insights-about-ywhae-role-in-the-tumor-development-and-metastasis-process
#13
Mariana Ferreira Leal, Helem Ferreira Ribeiro, Juan Antonio Rey, Giovanny Rebouças Pinto, Marília Cardoso Smith, Caroline Aquino Moreira-Nunes, Paulo Pimentel Assumpção, Leticia Martins Lamarão, Danielle Queiroz Calcagno, Raquel Carvalho Montenegro, Rommel Rodriguez Burbano
We previously observed reduced YWHAE (14-3-3ε) protein expression in a small set of gastric cancer samples. YWHAE may act as a negative regulator of the cyclin CDC25B, which is a transcriptional target of MYC oncogene. The understanding of YWHAE role and its targets is important for the better knowledge of gastric carcinogenesis. Thus, we aimed to evaluate the relationship among YWHAE, CDC25B, and MYC in vitro and in vivo. For this, we analyzed the YWHAE, CDC25B, and MYC expression in YWHA-silenced, CDC25B-silenced, and MYC-silenced gastric cancer cell lines, as well as in gastric cancer and non-neoplastic gastric samples...
November 16, 2016: Oncotarget
https://www.readbyqxmd.com/read/27847122/electrochemical-mineralization-of-cephalexin-using-a-conductive-diamond-anode-a-mechanistic-and-toxicity-investigation
#14
Douglas A C Coledam, Marília M S Pupo, Bianca F Silva, Adilson J Silva, Katlin I B Eguiluz, Giancarlo R Salazar-Banda, José M Aquino
The contamination of surface and ground water by antibiotics is of significant importance due to their potential chronic toxic effects to the aquatic and human lives. Thus, in this work, the electrochemical oxidation of cephalexin (CEX) was carried out in a one compartment filter-press flow cell using a boron-doped diamond (BDD) electrode as anode. During the electrolysis, the investigated variables were: supporting electrolyte (Na2SO4, NaCl, NaNO3, and Na2CO3) at constant ionic strength (0.1 M), pH (3, 7, 10, and without control), and current density (5, 10 and 20 mA cm(-2))...
November 12, 2016: Chemosphere
https://www.readbyqxmd.com/read/27835659/effects-of-high-intensity-training-of-professional-runners-on-myocardial-hypertrophy-and-subclinical-atherosclerosis
#15
Célia Regina de Oliveira Bittencourt, Maria Cristina de Oliveira Izar, Valdir Lauro Schwerz, Rui Manuel Dos Santos Póvoa, Henrique Andrade Rodrigues Fonseca, Marília Izar Helfenstein Fonseca, Henrique Tria Bianco, Carolina Nunes França, Carlos Eduardo Dos Santos Ferreira, Francisco Antonio Helfenstein Fonseca
To evaluate the effects of long-term exposure to high-intensity training among professional runners on cardiac hypertrophy and subclinical atherosclerosis. Prospective study included runners of both sexes (n = 52) and age and gender matched controls (n = 57), without classical cardiovascular risk factors. Ventricular hypertrophy was quantified by echocardiography by linear method and carotid intima-media thickness (cIMT) by 2-D images obtained by ultrasonography. Endothelial function was evaluated by flow-mediated dilation (FMD)...
2016: PloS One
https://www.readbyqxmd.com/read/27828602/ventral-abdominal-wall-defect-correction-in-rats-with-contaminated-meshes
#16
Paola Zarur Varella, Nicolau Gregori Czeczko, Manoel Alberto Prestes, Alexandre Eduardo Augustin Czeczko, Marília da Cruz Fagundes, Roberta Dombroski Petisco
PURPOSE: To investigate whether there is a difference between Marlex(r) and Dynamesh PP-light Marlex(r) meshes, in the abdominal wall defect correction, on rats in contaminated surgical site. METHODS: Twenty-eight Wistar rats were divided into two groups of 14, and four subgroups of seven animals. All subgroups underwent similar surgical procedure. One group received the mesh Marlex(r) and the other Dynamesh PP-light(r) for correction of the defect. Before implanting, the meshes went through a contamination process, on which was used standard solution containing 10 UFC of Escherichia coli...
October 2016: Acta Cirúrgica Brasileira
https://www.readbyqxmd.com/read/27828566/factors-associated-with-the-vulnerability-of-older-people-living-with-hiv-aids-in-belo-horizonte-mg-brazil
#17
Marília Borborema Rodrigues Cerqueira, Roberto Nascimento Rodrigues
The objective of this article was to identify the factors associated with the vulnerability of the elderly aged 60 years or older to HIV/AIDS, based on the perspective of older people living with the virus. Interviews were conducted with 20 participants, including 12 women and eight men, who were patients from a public hospital in Belo Horizonte, Minas Gerais, Brazil. The elderly interviewed had a low education level and low income, were currently or previously in a marital union, had perceptions and behaviors grounded in structural gender relations with power asymmetry and had a low capacity to respond to vulnerability...
November 2016: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/27815516/systemic-oxalosis-in-hyperoxaluria-type-1
#18
Marília Rodrigues, José Carlos Cardoso, José A P da Silva
No abstract text is available yet for this article.
November 3, 2016: Rheumatology
https://www.readbyqxmd.com/read/27815155/environmental-enrichment-protects-against-stress-induced-anxiety-role-of-glucocorticoid-receptor-erk-and-creb-signaling-in-the-basolateral-amygdala
#19
Leonardo S Novaes, Nilton Barreto Dos Santos, Rafaela F P Batalhote, Marília Brinati Malta, Rosana Camarini, Cristoforo Scavone, Carolina Demarchi Munhoz
Environmental enrichment (EE) is an experimental animal model that enhances an animal's opportunity to interact with sensory, motor, and social stimuli, compared to standard laboratory conditions. A prominent benefit of EE is the reduction of stress-induced anxiety. The relationship between stress and the onset of anxiety-like behavior has been widely investigated in experimental research, showing a clear correlation with structural changes in the hippocampus and basolateral amygdala (BLA). However, the mechanisms by which EE exerts its protective roles in stress and anxiety remain unclear, and it is not known whether EE reduces the effects of acute stress on animal behavior shortly following the cessation of stress...
November 1, 2016: Neuropharmacology
https://www.readbyqxmd.com/read/27814258/efficacy-of-nd-yag-and-gaalas-lasers-in-comparison-to-2-fluoride-gel-for-the-treatment-of-dentinal-hypersensitivity
#20
Marília De Lima Soares, Geane Bandeira Porciúncula, Mara Ilka Holanda Medeiros De Lucena, Luiz Alcino Monteiro Gueiros, Jair Carneiro Leão, Alessandra De Albuquerque Tavares Carvalho
Lasers demonstrate excellent therapeutic action and are often employed in dentistry for the treatment of diverse clinical conditions. The aim of this study was to compare the efficacy of neodymium-doped yttrium-aluminum-garnet (Nd:YAG) laser, gallium-aluminum-arsenide (GaAlAs) laser, and 2% neutral fluoride gel in the treatment of dentinal hypersensitivity. Twenty-three patients were evaluated, involving a total of 48 quadrants with at least 1 tooth with dentinal hypersensitivity (89 teeth total). Pain intensity was recorded on a visual analog scale at the time of clinical examination (baseline), immediately after treatment, and 1 week posttreatment...
November 2016: General Dentistry
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