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Talita B Gontijo, Rossimiriam P de Freitas, Guilherme F de Lima, Lucas C D de Rezende, Leandro F Pedrosa, Thaissa L Silva, Marilia O F Goulart, Bruno C Cavalcanti, Claudia Pessoa, Marina P Bruno, José R Corrêa, Flavio S Emery, Eufrânio N da Silva Júnior
For the first time, a fluorescent lapachone-based BODIPY was synthesised and characterised by NMR and mass spectrometry. Computational and electrochemical aspects, as well as cytotoxic activity and subcellular localisation, were studied. Confocal microscopy experiments indicated that the probe was a specific mitochondria-staining agent. These in-detail analyses were useful in understanding the cytotoxic effects and mechanism of action of this novel hybrid compound. This molecule constitutes a promising prototype owing to its potential biological activities and the new strategies aimed at mechanistic investigations in cells and in vivo, and opens up an interesting avenue of research...
October 24, 2016: Chemical Communications: Chem Comm
Marilia N Siebert, Jacó J Mattos, Clei E Piazza, Daína de Lima, Carlos Henrique A M Gomes, Cláudio M R de Melo, Afonso C D Bainy
Cytochrome P450 family 1 (CYP1) is involved in polycyclic aromatic hydrocarbons (PAHs) biotransformation. PAHs can induce CYP1 protein expression and enzyme activity, the latter being usually quantified as 7-ethoxyresorufin O-deethylase activity (EROD). The aim of this study was to characterize EROD activity in the bivalve mollusk Crassostrea brasiliana. EROD activity was evaluated in cytosolic and microsomal fractions of gills, digestive gland and mantle of C. brasiliana. No EROD activity was detected in mantle, but it was present in microsomal fraction of gills and digestive gland with NADPH as coenzyme...
October 18, 2016: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
João R Daniel, António J Santos, Marta Antunes, Marília Fernandes, Brian E Vaughn
We used stochastic actor-based models to test whether the developmental dynamics of friendships and antipathies in preschool peer groups (followed throughout three school years) were co-dependent. We combined choices from three sociometric tasks of 142 children to identify friendship and antipathy ties and used SIENA to model network dynamics. Our results show that different social processes drive the development of friendship and antipathy ties, and that they do not develop in association (i.e., friendship ties are not dependent on existing antipathies, and vice-versa)...
2016: Frontiers in Psychology
Luiza Cassiano, Juliano Pessan, Livia Comar, Flavia Levy, Cristiane Cardoso, Aline Dionisio, Michele Manarelli, Larissa Grizzo, Ana Carolina Magalhães, Marília Buzalaf
OBJECTIVES: This study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ. MATERIALS AND METHODS: Pre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7days in each phase. Each treatment comprised milk containing 2.5ppm fluoride daily (T1), or every other day (T2), 5...
October 13, 2016: Archives of Oral Biology
Jorge Mejia, Ana Claudia Camargo Miranda, Ana Claudia Ranucci Durante, Larissa Rolim de Oliveira, Marycel Rosa Felisa Figols de Barboza, Katerin Taboada Rosell, Daniele Pereira Jardim, Alexandre Holthausen Campos, Marilia Alves Dos Reis, Marcela Forli Catanoso, Orfa Yineth Galvis-Alonso, Francisco Romero Cabral
Objective: To present the result of upgrading a clinical gamma-camera to be used to obtain in vivo tomographic images of small animal organs, and its application to register cardiac, renal and neurological images. Methods: An updated version of the miniSPECT upgrading device was built, which is composed of mechanical, electronic and software subsystems. The device was attached to a Discovery VH (General Electric Healthcare) gamma-camera, which was retired from the clinical service and installed at the Centro de Imagem Pré-Clínica of the Hospital Israelita Albert Einstein...
July 2016: Einstein
Fernanda Wisnieski, Mariana Ferreira Leal, Danielle Queiroz Calcagno, Leonardo Caires Santos, Carolina Oliveira Gigek, Elizabeth Suchi Chen, Ricardo Artigiani, Sâmia Demachki, Paulo Pimentel Assumpção, Laércio Gomes Lourenço, Rommel Rodriguez Burbano, Marília Cardoso Smith
Different from genetic alterations, the reversible nature of epigenetic modifications provides an interesting opportunity for the development of clinically relevant therapeutics in different tumors. In this study, we aimed to screen and validate candidate genes regulated by the epigenetic marker associated with transcriptional activation, histone acetylation, in gastric cancer (GC). We first compared gene expression profile of trichostatin A-treated and control GC cell lines using microarray assay. Among the 55 differentially expressed genes identified in this analysis, we chose the up-regulated genes BMP8B and BAMBI for further analyzes, that included mRNA and histone acetylation quantification in paired GC and nontumor tissue samples...
October 17, 2016: Journal of Cellular Biochemistry
Bruno A Randi, Marília F Felício, Carolina S Lázari, Maria Irma S Duarte, Ilana Halpern, Yeh-Li Ho
No abstract text is available yet for this article.
November 1, 2016: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Alessia Cicenia, Floriana Santangelo, Lucrezia Gambardella, Lucia Pallotta, Valerio Iebba, Annunziata Scirocco, Massimo Marignani, Guglielmo Tellan, Marilia Carabotti, Enrico Stefano Corazziari, Serena Schippa, Carola Severi
BACKGROUND: Some beneficial effects of probiotics may be due to secreted probiotic-derived factors, identified as "postbiotic" mediators. The aim of this study was to evaluate whether supernatants harvested from Lactobacillus rhamnosus GG (LGG) cultures (ATCC53103 strain) protect colonic human smooth muscle cells (HSMCs) from lipopolysaccharide (LPS)-induced myogenic damage. MATERIALS AND METHODS: LGG was grown in de Man, Rogosa, Share medium at 37°C and samples were collected in middle and late exponential, stationary, and overnight phases...
November 2016: Journal of Clinical Gastroenterology
Tania Mercedes López Martínez, Marília Leão Goettems, Marina Sousa Azevedo, Marcos Britto Correa, Flávio Fernando Demarco, Ana Regina Romano
The aim of this study was to assess the prevalence of black stains (BS) and factors associated with their occurrence and to investigate the association of BS with caries experience after adjusting for other risk indicators. A school-based, cross-sectional study using multistage sampling of children aged 10-12 years from 20 private and public schools in Pelotas/Southern Brazil was conducted (n = 706). Children were evaluated for BS and dental caries (DMFT/dmft index) and socioeconomic information was collected by questionnaire...
October 10, 2016: Brazilian Oral Research
Fabíula Francisca Abreu, Ana Carla Araujó de Souza, Simone Aparecida Teixeira, Antônio Garcia Soares, Daiane Franco Teixeira, Rosilene Calazans Soares, Marilia Trindade Santana, Sandra Lauton Santos, Soraia Kátia Pereira Costa, Marcelo Nicolas Muscará, Enilton Aparecido Camargo
INTRODUCTION: Acute pancreatitis (AP) may be severe and cause hospitalization or death, and the available treatment is insufficient to control pancreatic inflammation and pain. Rutin is a natural flavonoid with the potential to treat AP via anti-inflammatory, antinociceptive and antioxidant activities. AIM: This study investigated the beneficial effects of rutin on experimental AP induced by L-arginine administration in mice. METHODS: The L-arginine-induced AP model was used in Swiss mice (n = 6-8)...
October 13, 2016: Free Radical Research
Diego Soares Carvalho, Marilia Melo Diniz, André Abour Haidar, Maria de Fátima Cavanal, Eduardo da Silva Alves, Angelo Rafael Carpinelli, Frida Zaladek Gil, Aparecida Emiko Hirata
Maternal hyperglycemia can result in defects in glucose metabolism and pancreatic β-cell function in offspring. The purpose of this study was to evaluate the impact of maternal diabetes mellitus on pancreatic islets, muscle and adipose tissue of the offspring, with or without oral l-Arginine supplementation. The induction of diabetes was performed using streptozotocin (60mg/kg). Animals were studied at 3 months of age and treatment (sucrose or l-Arginine) was administered from weaning. We observed that l-Arg improved insulin sensitivity in the offspring of diabetic mothers (DA), reflected by higher insulin-induced phosphorylation of Akt in muscle and adipose tissue...
October 4, 2016: European Journal of Pharmacology
Mariana de Almeida Lourenço, Izabela Roma, Marcos Renato de Assis
OBJECTIVE: To evaluate the occurrence of falls reported by rheumatoid arthritis (RA) patients and its relation to disease activity, functional capacity and physical fitness. MATERIAL AND METHODS: A cross-sectional study constituted by a sample of 97 RA patients from the city of Marília (SP) from 2012 to 2013, were assessed for disease activity. Instruments validated for Brazilian population in order to evaluate physical and functional capacity were used. Data analysis was carried out with descriptive statistics, Spearman correlation and Chi-squared test, considering p<0...
September 22, 2016: Revista Brasileira de Reumatologia
Rita Terezinha de Oliveira Carneiro, Maíza Alves Lopes, Marília Lordelo Cardoso Silva, Verônica da Silva Santos, Volnei Brito de Souza, Aurizangela Oliveira de Sousa, Carlos Priminho Pirovani, Maria Gabriela Bello Koblitz, Raquel Guimarães Benevides, Aristóteles Góes-Neto
White-rot basidiomycetes are the organisms that decompose lignin most efficiently and Trametes villosa is a promising species for the ligninolytic enzymes production. There are several publications on Trametes villosa applications for lignin degradation regarding the expression and secretion of laccase (Lac) and manganese peroxidase (MnP) but no reports on the identification and characterization of lignin peroxidase (LiP), a relevant enzyme for the efficient breaking down of lignin. The object of this study was to identify and partially characterize, for the first time, gDNA, mRNA and the corresponding lignin peroxidase (TvLiP) protein from a strain of Trametes villosa CCMB561 from the Brazilian semi-arid region...
October 6, 2016: Journal of Microbiology and Biotechnology
Hisaaki Shinohara, Takeshi Nagashima, Marilia I Cascalho, Tomohiro Kurosaki
TAK1 (MAP3K7) mediation of the IκB kinase (IKK) complex-nuclear factor-κB (NF-κB) pathway is crucial for the activation of immune response and to perpetuate inflammation. Although progress has been made to understand TAK1 function in the B-cell receptor (BCR) signaling, the physiological roles of TAK1 in B-cell development, particularly in the bone marrow (BM), remain elusive. Previous studies suggested that the IKK complex is required for the development of immunoglobulin light chain λ-positive B cells, but not for receptor editing...
October 3, 2016: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Mykaella Andrade de Araújo, Thalita Ewellyn Batista Sales Marques, Shirley Octacílio-Silva, Carmem Lúcia de Arroxelas-Silva, Marília Gabriella Alves Goulart Pereira, José Eduardo Peixoto-Santos, Ludmyla Kandratavicius, João Pereira Leite, Norberto Garcia-Cairasco, Olagide Wagner Castro, Marcelo Duzzioni, Geraldo Aleixo Passos, Maria Luisa Paçó-Larson, Daniel Leite Góes Gitaí
The involvement of miRNA in mesial temporal lobe epilepsy (MTLE) pathogenesis has increasingly become a focus of epigenetic studies. Despite advances, the number of known miRNAs with a consistent expression response during epileptogenesis is still small. Addressing this situation requires additional miRNA profiling studies coupled to detailed individual expression analyses. Here, we perform a miRNA microarray analysis of the hippocampus of Wistar rats 24 hours after intra-hippocampal pilocarpine-induced Status Epilepticus (H-PILO SE)...
2016: PloS One
Marília Daniella Machado Araújo Cavalcante, Liliana Müller Larocca, Maria Marta Nolasco Chaves, Márcia Regina Cubas, Laura Christina Macedo Piosiadlo, Verônica de Azevedo Mazza
OBJECTIVE: To analyze the use of nursing terminology as an instrument of the nursing work process in Collective Health. METHOD: Exploratory case study. For data collection was conducted a group interview with 24 nurses working in health units of a municipality in south central Paraná, Brazil. Data were analyzed in the light of interdependence between the structural, particular and singular dimensions contained in the Theory of Nursing Praxis Intervention in Collective Health...
July 2016: Revista da Escola de Enfermagem da U S P
Lídia Teixeira, Ana Rita Fonseca, Gisela Eugénio, Marília Rodrigues, Nikita Khmelinskii, Sílvia Fernandes, Sofia Serra, Tiago Costa, Maria José Santos
INTRODUCTION: Infections are a major cause of morbidity and mortality in systemic inflammatory rheumatic diseases and the management of infectious complications in patients under biological therapies deserves particular attention. OBJECTIVE: Develop evidence-based recommendations for the management of infections in rheumatic patients receiving biological therapies. METHODS: A search in PubMed (until 10 November 2014) and EMBASE (until 20 December 2014) databases was performed...
August 6, 2016: Acta Reumatológica Portuguesa
Kelly Cristina da Silva Oliveira, Taíssa Maíra Thomaz Araújo, Camila Inagaki Albuquerque, Gabriela Alcantara Barata, Carolina Oliveira Gigek, Mariana Ferreira Leal, Fernanda Wisnieski, Fernando Augusto Rodrigues Mello Junior, André Salim Khayat, Paulo Pimentel de Assumpção, Rommel Mário Rodriguez Burbano, Marília Cardoso Smith, Danielle Queiroz Calcagno
Alterations in epigenetic control of gene expression play an important role in many diseases, including gastric cancer. Many studies have identified a large number of upregulated oncogenic miRNAs and downregulated tumour-suppressor miRNAs in this type of cancer. In this review, we provide an overview of the role of miRNAs, pointing to their potential to be useful as diagnostic and/or prognostic biomarkers in gastric cancer. Moreover, we discuss the influence of polymorphisms and epigenetic modifications on miRNA activity...
September 21, 2016: World Journal of Gastroenterology: WJG
Luisa C Domingos, Matheus Vilardo L Moreira, Kelly M Keller, Fernando Antônio B Viana, Marília M Melo, Benito Soto-Blanco
The fourth-generation fluoroquinolones are widely used as ophthalmic antimicrobials. This study aimed to validate a new analytical technique for simultaneous quantification of gatifloxacin, moxifloxacin, and besifloxacin concentrations in the cornea and aqueous humor by liquid chromatography (LC) coupled to quadrupole time-of-flight mass spectrometry (QTOF/MS) at 10min and 1h after instillation of topical ophthalmic antimicrobial suspensions. It was used twenty-two male dogs without ocular lesions verified by ophthalmic and histologic examinations...
September 23, 2016: Journal of Pharmacological and Toxicological Methods
Marilia Cascalho
No abstract text is available yet for this article.
August 17, 2016: Journal of Heart and Lung Transplantation
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