keyword
MENU ▼
Read by QxMD icon Read
search

Small rna

keyword
https://www.readbyqxmd.com/read/29050315/the-activation-of-src-family-kinases-and-focal-adhesion-kinase-with-the-loss-of-the-amplified-mutated-egfr-gene-contributes-to-the-resistance-to-afatinib-erlotinib-and-osimertinib-in-human-lung-cancer-cells
#1
Yuichi Murakami, Kahori Sonoda, Hideyuki Abe, Kosuke Watari, Daiki Kusakabe, Koichi Azuma, Akihiko Kawahara, Jun Akiba, Chitose Oneyama, Jonathan A Pachter, Kazuko Sakai, Kazuto Nishio, Michihiko Kuwano, Mayumi Ono
Second- and third-generation inhibitors of epidermal growth factor receptor (EGFR) tyrosine kinase activity (EGFR-TKIs) are improving the treatment of patients with non-small cell lung cancer. Here we established two sublines (BR1-8 and BR2-3) resistant to a second-generation inhibitor, afatinib, from the human lung cancer cell line HCC827 that harbors a mutation that activates the tyrosine kinase activity of EGFR. These afatinib-resistant sublines were resistant to first-generation EGFR-TKIs, gefitinib and erlotinib, and a third-generation EGFR-TKI, osimertinib...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050267/reversible-regulation-of-orc2-sumoylation-by-pias4-and-senp2
#2
Ronghua Wang, Fangming Liu, Yongxu Zhao, Dan Wu, Lihan Chen, Edward T H Yeh, Chao Huang
The small ubiquitin-related modifier (SUMO) system is essential for smooth progression of cell cycle at the G2/M phase. Many centromeric proteins are reversibly SUMOylated to ensure proper chromosome segregation at the mitosis. SUMOylation of centromeric Origin Recognition Complex subunit 2 (ORC2) at the G2/M phase is essential in maintaining genome integrity. However, how ORC2 SUMOylation is regulated remains largely unclear. Here we show that ORC2 SUMOylation is reversibly controlled by SUMO E3 ligase PIAS4 and De-SUMOylase SENP2...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050263/the-prognostic-value-of-osteopontin-in-limited-stage-small-cell-lung-cancer-patients-and-its-mechanism
#3
Fanglei Liu, Chunxue Bai, Zhongliang Guo
Osteopontin (OPN) is known to be overexpressed in numerous carcinomas. Although abundant OPN has been reported to be correlated with poor survival in non-small cell lung cancer (NSCLC) but their clinical and prognostic significance in SCLC remains unknown. In this study, RNA-sequencing was used to obtain gene expression data in SCLC tissue samples and OPN expression levels were then investigated using qPCR, immunohistochemical and Western blot. We found OPN was one of the most upregulated genes. Besides, the correlation of OPN with tumor clinicopathological characteristics was evaluated and we found OPN was associated with advanced tumor stages...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050246/nrf2-regulated-metabolic-gene-signature-as-a-prognostic-biomarker-in-non-small-cell-lung-cancer
#4
Akhileshwar Namani, Qin Qin Cui, Yihe Wu, Hongyan Wang, Xiu Jun Wang, Xiuwen Tang
Mutations in Kelch-like ECH-associated protein 1 (KEAP1) cause the aberrant activation of nuclear factor erythroid-derived 2-like 2 (NRF2), which leads to oncogenesis and drug resistance in lung cancer cells. Our study was designed to identify the genes involved in lung cancer progression targeted by NRF2. A series of microarray experiments in normal and cancer cells, as well as in animal models, have revealed regulatory genes downstream of NRF2 that are involved in wide variety of pathways. Specifically, we carried out individual and combinatorial microarray analysis of KEAP1 overexpression and NRF2 siRNA-knockdown in a KEAP1 mutant-A549 non-small cell lung cancer (NSCLC) cell line...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050214/prox1-represses-il-2-gene-expression-by-interacting-with-nfat2
#5
Shujie Zhang, Ning Yu, Linfang Wang, Yanfeng Liu, Yuying Kong, Jing Liu, Youhua Xie
Interleukin-2 (IL-2) is critical for T lymphocyte activation and regulated by many transcriptional factors. Prospero-related homeobox 1 (Prox1) is a multifunctional transcription factor, which can work as either a transcriptional activator or repressor depending on the cellular and developmental environment. We previously reported the Prox1 expression in T cells, raising the possibility of Prox1 involvement in the regulation of T cell function and IL-2 production. Here we demonstrated that the Prox1 expression in CD4+ T cells was downregulated by T cell receptor (TCR) activation...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050074/-clinicopathologic-characteristics-and-prognosis-of-neoplastic-cell-rich-mixed-cellularity-classic-hodgkin-lymphoma
#6
W H Hou, P Wei, J L Xie, Y Y Zheng, X G Zhou
Objective: To investigate the clinicopathologic characteristics of neoplastic cell-rich mixed cellularity classical Hodgkin lymphoma(MCCHL-R) and to compare the prognosis with typical mixed cellularity classic Hodgkin lymphoma(MCCHL). Methods: Fifty-four patients with MCCHL-R(the tumor cells >10%) and 65 patients with typical MCCHL identified from 1 721 Hodgkin lymphomas were reviewed to compare the clinicopathological characteristics including morphologic and immunophenotypic features, EBV infection status, clinical therapy and overall survival...
October 8, 2017: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29049916/immune-cell-type-fingerprints-at-the-basis-of-outcome-diversity-of-human-infection
#7
REVIEW
Shelly Hen-Avivi, Roi Avraham
Despite the availability of antibiotics and immunization, infectious diseases remain a major cause of malignancy and death worldwide. Yet, it is well documented that for most infectious agents, clinical disease develops in only a small minority of infected individuals. There is, in fact, great heterogeneity in infection outcome, from complete clearance of the pathogen to severe illness. Understanding this variation remains elusive, despite its great potential to equip us with new tools for the treatment of infectious diseases...
October 16, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29049831/improving-myocardial-fractional-flow-reserve-in-coronary-atherosclerosis-via-cx37-gene-silence-a-preclinical-validation-study-in-pigs
#8
Suxia Guo, Ying Yang, Zhenyu Yang, Huayan You, Yunke Shi, Zhao Hu, Zhaohui Meng, Jianming Xiao
OBJECTIVES: The purpose of this study was to evaluate the effect of CX37 gene silence on myocardial fractional flow reserve (FFR). METHODS: A total of 90 male pigs were randomly divided into saline, mock and 3 different doses (5, 10 and 20 µl) of CX37 viral suspension groups that could induce coronary plaque formation with high-fat diet. After performing myocardial FFR by intravascular ultrasound, different doses of CX37 viral suspension, saline and mock small interfering RNA (siRNA) were transfected into the related coronary...
July 31, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29049324/dynamic-microbial-populations-along-the-cuyahoga-river
#9
Matthew V Cannon, Joseph Craine, James Hester, Amanda Shalkhauser, Ernest R Chan, Kyle Logue, Scott Small, David Serre
The study of the microbial communities has gained traction in recent years with the advent of next-generation sequencing with, or without, PCR-based amplification of the 16S ribosomal RNA region. Such studies have been applied to topics as diverse as human health and environmental ecology. Fewer studies have investigated taxa outside of bacteria, however. We present here data demonstrating the utility of studying taxa outside of bacteria including algae, diatoms, archaea and fungi. Here, we show how location along the Cuyahoga River as well as a transient rainfall event heavily influence the microbial composition...
2017: PloS One
https://www.readbyqxmd.com/read/29048679/enhanced-wild-type-p53-expression-by-small-activating-rna-dsp53-285-induces-cell-cycle-arrest-and-apoptosis-in-pheochromocytoma-cell-line-pc12
#10
Dengqiang Lin, Li Meng, Feifei Xu, Jianpo Lian, Yunze Xu, Xin Xie, Xiaojing Wang, Hongchao He, Chenghe Wang, Yu Zhu
Malignant pheochromocytoma (PHEO) is diagnosed only when metastasis has occurred, making it less likely for patients to obtain the benefits of traditional chemotherapy. Anti-oncogene TP53 mutation has been detected in PHEO and is possibly related to disease progression. However, whether the upregulation of wild-type TP53 has antitumoral effects on PHEO remains completely unknown. In the present study, we used RNA activation (RNAa) technique to upregulate the expression of wild-type TP53 by transfecting synthetic dsP53‑285 into PHEO cell line PC12...
September 25, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29048676/decreased-expression-of-the-augmenter-of-liver-regeneration-results-in-growth-inhibition-and-increased-chemosensitivity-of-acute-t-lymphoblastic-leukemia-cells
#11
Yan Shen, Qi Liu, Shifeng Lou, Yun Luo, Hang Sun, Hanqing Zeng, Jianchuan Deng
Augmenter of liver regeneration (ALR) plays crucial roles in cell survival and growth. Previous studies have demonstrated that ALR exerts a protective effect on toxic agent‑induced cell death in acute T lymphoblastic leukemia cells and ALR knockdown can sensitize cancer cells to radiation. However, the biological functions of ALR against drug resistance in T-cell acute lymphoblastic leukemia are mostly unknown. In the present study, we investigated the effect of small interfering RNA (siRNA)-induced ALR silencing on cell proliferation and sensitivity to vincristine (VCR) of Jurkat cells...
September 21, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29048663/suppression-of-letm1-by-sirna-inhibits-cell-proliferation-and-invasion-of-bladder-cancer-cells
#12
Bisheng Huang, Jingwei Zhang, Xiaolu Zhang, Chi Huang, Guanghui Hu, Saiyang Li, Tiancheng Xie, Mengnan Liu, Yunfei Xu
The leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is highly expressed in many human malignancies and is correlated with poor prognosis. However, the function of LETM1 in bladder cancer still remains unknown. In the present study, we analyzed the expression levels of LETM1 in bladder cancer tissues and non-cancerous tissues as well as in four bladder cancer cell lines (T24, EJ, 5637 and J82) and a human bladder epithelial immortalized cell line SV-HUC-1. Small interfering RNA (siRNA) was employed to knockdown the expression of LETM1 in the T24 cells...
September 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29048560/targeting-the-mesenchymal-subtype-in-glioblastoma-and-other-cancers-via-inhibition-of-diacylglycerol-kinase-alpha
#13
Inan Olmez, Shawn Love, Aizhen Xiao, Laryssa Manigat, Peyton Randolph, Brian D McKenna, Brian P Neal, Salome Boroda, Ming Li, Breanna Brenneman, Roger Abounader, Desiree Floyd, Jeongwu Lee, Ichiro Nakano, Jakub Godlewski, Agnieszka Bronisz, Erik P Sulman, Marty Mayo, Daniel Gioeli, Michael Weber, Thurl E Harris, Benjamin Purow
Background: The mesenchymal phenotype in glioblastoma (GBM) and other cancers drives aggressiveness and treatment resistance, leading to therapeutic failure and recurrence of disease. Currently, there is no successful treatment option available against the mesenchymal phenotype. Methods: We classified patient-derived GBM stem cell lines into 3 subtypes: proneural, mesenchymal, and other/classical. Each subtype's response to the inhibition of diacylglycerol kinase alpha (DGKα) was compared both in vitro and in vivo...
June 29, 2017: Neuro-oncology
https://www.readbyqxmd.com/read/29048434/common-microrna-mrna-interactions-exist-among-distinct-porcine-ipsc-lines-independent-of-their-metastable-pluripotent-states
#14
Shiqiang Zhang, Youlong Xie, Hongxia Cao, Huayan Wang
Previous evidences have proved that porcine-induced pluripotent stem cells (piPSCs) could be induced to distinctive metastable pluripotent states. This raises the issue of whether there is a common transcriptomic profile existing among the piPSC lines at distinctive state. In this study, we performed conjoint analysis of small RNA-seq and mRNA-seq for three piPSC lines which represent LIF dependence, FGF2 dependence and LFB2i dependence, respectively. Interestingly, we found there are 16 common microRNAs which potentially target 13 common mRNAs among the three piPSC lines...
August 31, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29046688/global-analysis-of-small-rna-dynamics-during-seed-development-of-picea-glauca-and-arabidopsis-thaliana-populations-reveals-insights-on-their-evolutionary-trajectories
#15
Yang Liu, Yousry A El-Kassaby
While DNA methylation carries genetic signals and is instrumental in the evolution of organismal complexity, small RNAs (sRNAs), ~18-24 ribonucleotide (nt) sequences, are crucial mediators of methylation as well as gene silencing. However, scant study deals with sRNA evolution via featuring their expression dynamics coupled with species of different evolutionary time. Here we report an atlas of sRNAs and microRNAs (miRNAs, single-stranded sRNAs) produced over time at seed-set of two major spermatophytes represented by populations of Picea glauca and Arabidopsis thaliana with different seed-set duration...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29046684/novel-insights-into-mirna-regulation-of-storage-protein-biosynthesis-during-wheat-caryopsis-development-under-drought-stress
#16
Xin-Yu Chen, Yang Yang, Li-Ping Ran, Zhao-di Dong, Er-Jin Zhang, Xu-Run Yu, Fei Xiong
Drought stress is a significant abiotic stress factor that affects wheat yield and quality. MicroRNA (miRNA) plays an important role in regulating caryopsis development in response to drought stress. However, little is known about the expression characteristics of miRNAs and how they regulate protein accumulation in wheat caryopsis under drought stress. To address this, two small RNA libraries of wheat caryopsis under control and drought stress conditions were constructed and sequenced. A total of 125 miRNAs were identified in the two samples, of which 110 were known and 15 were novel...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29046449/gga-mir-130b-suppresses-infectious-bursal-disease-virus-replication-via-targeting-the-viral-genome-and-cellular-socs5
#17
Mengjiao Fu, Bin Wang, Xiang Chen, Zhiyuan He, Yongqiang Wang, Xiaoqi Li, Hong Cao, Shijun J Zheng
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression post transcriptionally through silencing or degrading its targets, playing important roles in immune response. However, the role of miRNAs in host response against infectious bursal disease virus (IBDV) infection is not clear. In this study, we show that the expression of a series of miRNAs was significantly altered in DF-1 cells after infectious bursal disease virus (IBDV) infection. We found that gga-miR-130b inhibited IBDV replication via targeting the specific sequence of IBDV segment A and enhanced the expression of IFN-β by targeting Suppressors of Cytokine Signaling 5(SOCS5) in host cells...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046445/the-impacts-of-genome-wide-analyses-on-our-understanding-of-human-herpesvirus-diversity-and-evolution
#18
Daniel W Renner, Moriah L Szpara
Until fairly recently, genome-wide evolutionary dynamics and within-host diversity were more commonly examined in the context of small viruses, than large double-stranded DNA viruses such as herpesviruses. The high mutation rates and more compact genomes of RNA viruses have inspired the investigation of population dynamics for these species, and recent data now suggest that herpesviruses might also be considered candidates for population modeling. High-throughput sequencing (HTS) and bioinformatics have expanded our understanding of herpesviruses through genome-wide comparisons of sequence diversity, recombination, allele frequency, and selective pressures...
October 18, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29046400/ribosome-biogenesis-modulates-ty1-copy-number-control-in-saccharomyces-cerevisiae
#19
Hyo Won Ahn, Jessica M Tucker, Joshua A Arribere, David J Garfinkel
Transposons can impact the host genome by altering gene expression and participating in chromosome rearrangements. Therefore, organisms evolved different ways to minimize the level of transposition. In Saccharomyces cerevisiae and its close relative S. paradoxus, Ty1 copy number control (CNC) is mediated by the self-encoded restriction factor p22, which is derived from the GAG capsid gene and inhibits virus-like particle (VLP) assembly and function. Based on secondary screens of Ty1 cofactors, we identified LOC1, a RNA localization/ribosome biogenesis gene that affects Ty1 mobility predominantly in strains harboring Ty1 elements...
October 18, 2017: Genetics
https://www.readbyqxmd.com/read/29046345/endoplasmic-reticulum-transmembrane-proteins-zdhhc1-and-sting-both-act-as-direct-adaptors-for-irf3-activation-in-teleost
#20
Xiaowen Xu, Meifeng Li, Zhen Wu, Haizhou Wang, Liqiang Wang, Keyi Huang, Xiancheng Liu, Qunhao Hou, Gang Lin, Chengyu Hu
IFN regulatory factor (IRF)3 is a central regulator for IFN-β expression in different types of pathogenic infections. Mammals have various pathogenic sensors that are involved in monitoring pathogen intrusions. These sensors can trigger IRF3-mediated antiviral responses through different pathways. Endoplasmic reticulum-associated proteins stimulator of IFN gene (STING) and zinc finger DHHC-type containing 1 (ZDHHC1) are critical mediators of IRF3 activation in response to viral DNA infections. In this study, grass carp STING and ZDHHC1 were found to have some similar molecular features and subcellular localization, and both were upregulated upon stimulation with polyinosinic:polycytidylic acid, B-DNA, or Z-DNA...
October 18, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
keyword
keyword
6911
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"