Kristina R Rivera, R Jarrett Bliton, Joseph Burclaff, Michael J Czerwinski, Jintong Liu, Jessica M Trueblood, Caroline M Hinesley, Keith A Breau, Halston E Deal, Shlok Joshi, Vladimir A Pozdin, Ming Yao, Amanda L Ziegler, Anthony T Blikslager, Michael A Daniele, Scott T Magness
BACKGROUND & AIMS: Hypoxia in the intestinal epithelium can be caused by acute ischemic events or chronic inflammation where immune cell infiltration produces 'inflammatory hypoxia' starving the mucosa of oxygen. The epithelium has the capacity to regenerate after some ischemic and inflammatory conditions suggesting intestinal stem cells (ISCs) are highly tolerant to acute and chronic hypoxia; however, the impact of hypoxia on human ISC (hISC) function has not been reported. Here we present a new microphysiological system (MPS) to investigate how hypoxia affects hISCs from healthy donors and test the hypothesis that prolonged hypoxia modulates how hISCs respond to inflammation-associated interleukins...
August 8, 2023: Cellular and Molecular Gastroenterology and Hepatology