A A Gureeva, G Iu Grigorian, V O Rybin, N A Pereverzev, I A Lapaeva
The modified method for the isolation and purification of B. pertussis toxin has been proposed. Chromatography with the use of hydroxylapatite and lentil lectin--Sepharose 4B has permitted the isolation of the preparation purified 600 times. Its molecular weight is about 90,000. The preparation has been found to possess leukocytosis-stimulating, histamine-sensitising and hemagglutinating activity. Electrophoretic analysis has revealed that the isolated substance consists of four subunits with molecular weights 28,400, 24,300, 21,800 and 15,200...
July 1986: Zhurnal Mikrobiologii, Epidemiologii, i Immunobiologii