keyword
MENU ▼
Read by QxMD icon Read
search

sn-2

keyword
https://www.readbyqxmd.com/read/27933292/nitric-oxide-modulates-postnatal-bone-marrow-derived-mesenchymal-stem-cell-migration
#1
John W Fuseler, Mani T Valarmathi
Nitric oxide (NO) is a small free-radical gas molecule, which is highly diffusible and can activate a wide range of downstream effectors, with rapid and widespread cellular effects. NO is a versatile signaling mediator with a plethora of cellular functions. For example, NO has been shown to regulate actin, the microfilament, dependent cellular functions, and also acts as a putative stem cell differentiation-inducing agent. In this study, using a wound-healing model of cellular migration, we have explored the effect of exogenous NO on the kinetics of movement and morphological changes in postnatal bone marrow-derived mesenchymal stem cells (MSCs)...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27932289/peroxiredoxin-6-in-the-repair-of-peroxidized-cell-membranes-and-cell-signaling
#2
REVIEW
Aron B Fisher
Peroxiredoxin 6 represents a widely distributed group of peroxiredoxins that contain a single conserved cysteine in the protein monomer (1-cys Prdx). The cys when oxidized to the sulfenic form is reduced with glutathione (GSH) catalyzed by the π isoform of GSH-S-transferase. Three enzymatic activities of the protein have been described:1) peroxidase with H2O2, short chain hydroperoxides and phospholipid hydroperoxides as substrates; 2) phospholipase A2 (PLA2); and 3) lysophosphatidylcholine acyl transferase (LPCAT)...
December 5, 2016: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/27917381/arachidonoyl-specific-diacylglycerol-kinase-%C3%AE%C2%B5-and-the-endoplasmic-reticulum
#3
REVIEW
Tomoyuki Nakano, Hirooki Matsui, Toshiaki Tanaka, Yasukazu Hozumi, Ken Iseki, Kaneyuki Kawamae, Kaoru Goto
The endoplasmic reticulum (ER) comprises an interconnected membrane network, which is made up of lipid bilayer and associated proteins. This organelle plays a central role in the protein synthesis and sorting. In addition, it represents the synthetic machinery of phospholipids, the major constituents of the biological membrane. In this process, phosphatidic acid (PA) serves as a precursor of all phospholipids, suggesting that PA synthetic activity is closely associated with the ER function. One enzyme responsible for PA synthesis is diacylglycerol kinase (DGK) that phosphorylates diacylglycerol (DG) to PA...
2016: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/27894770/activity-based-targeting-of-secretory-phospholipase-a2-enzymes-a-fatty-acid-binding-protein-assisted-approach
#4
Amir Keshavarz, Ligia Zelaya, Jasmeet Singh, Radha Ranganathan, Joseph Hajdu
Syntheses and enzymological characterization of fluorogenic substrate probes targeting secretory phospholipase A2 (sPLA2) for detection and quantitative assays are presented. Three fluorogenic phosphatidylcholine analogs PC-1, PC-2, and PC-3 each containing the duo of 7-mercapto-4-methyl-coumarin fluorophore and 2,4-dinitroanaline quencher on either tail were synthesized from (R)-3-amino-1,2-propanediol and R-(-)-2,2-dimethyl-1,3-dioxolane-4-methanol. These small reporter groups are advantageous in preserving natural membrane integrity...
November 25, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27894769/identification-of-molecular-species-of-monoalkyldiacylglycerol-from-the-squid-berryteuthis-magister-using-liquid-chromatography-apci-high-resolution-mass-spectrometry
#5
Viacheslav G Rybin, Andrey B Imbs, Darja A Demidkova, Ekaterina V Ermolenko
Monoalkyldiacylglycerol (MADAG) is an important lipid class in mollusks, corals, starfishes, and some species of zooplankton. Up to 80% of liver oils of squids and sharks are comprised of MADAG. Except for one fish species, there are no data on the composition of MADAG molecular species of marine organisms. The molecular species of MADAG obtained from digestive glands of the deep-sea squid Berryteuthis magister were identified. High-performance liquid chromatography (HPLC) and tandem high-resolution mass spectrometry (HRMS) with atmospheric pressure chemical ionization (APCI) were applied...
November 25, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27892866/effectiveness-of-a-bhv-1-befv-bivalent-vaccine-against-bovine-herpesvirus-type-1-infection-in-cattle
#6
Yao-Chi Chung, Hsiu-Yen Shen, Li-Ting Cheng, Shyh-Shyan Liu, Chun-Yen Chu
Bovine herpesvirus type 1 (BHV-1) causes acute febrile respiratory diseases (infectious bovine rhinotracheitis, IBR), decreased milk production, weight loss and abortion. Bovine ephemeral fever virus (BEFV) causes acute febrile respiratory disease, with pulmonary emphysema and pulmonary edema as the main signs. These viruses infect domesticated herds and lead to significant economic losses. In our previous study, an inactivated BHV-1 and BEFV bivalent vaccine was formulated with water-in-oil-in-water adjuvant, and vaccine efficacy was evaluated in guinea pigs...
October 13, 2016: Research in Veterinary Science
https://www.readbyqxmd.com/read/27873162/metabolism-of-sn-1-3-monoacylglycerol-and-sn-2-monoacylglycerol-in-caecal-enterocytes-and-hepatocytes-of-brown-trout-salmo-trutta
#7
Keshuai Li, Rolf Erik Olsen
sn-2-Monoacylglycerol (2-MAG) and sn-1(3)-monoacylglycerol [1(3)-MAG] are important but yet little studied intermediates in lipid metabolism. The current study compared the metabolic fate of 2-MAG and 1(3)-MAG in isolated caecal enterocytes and hepatocytes of brown trout (Salmo trutta). 1(3)-Oleoyl [9,10-3H(N)]-glycerol and 2-Oleoyl [9,10-3H(N)]-glycerol were prepared by pancreatic lipase digestion of triolein [9,10-3H(N)]. The 1(3)-MAG and 2-MAG were efficiently absorbed by enterocytes and hepatocytes at similar rates...
November 21, 2016: Lipids
https://www.readbyqxmd.com/read/27864058/a-novel-alkaline-surfactant-stable-keratinase-with-superior-feather-degrading-potential-based-on-library-screening-strategy
#8
Chang Su, Jin-Song Gong, Rong-Xian Zhang, Li-Yan Tao, Wen-Fang Dou, Dan-Dan Zhang, Heng Li, Zhen-Ming Lu, Zheng-Hong Xu, Jin-Song Shi
A novel keratinase was mined and expressed in Escherichia coli BL21 (DE3) via function-driven screening with fosmid library. The catalytic properties of purified keratinase were investigated in detail following enzyme purification. The recombinant keratinase was purified to homogeneity with an estimated molecular weight of 26kDa using nickel affinity chromatography, of which the optimal reaction pH and temperature were 10.0 and 55°C, respectively. It could remain stable at pH 5.0-12.0 and 40-60°C. Metal ions such as Ca(2+), Mn(2+), Ag(+), Na(+), Mg(2+), Li(+), Sn(2+) (1mM) displayed positive influence on keratinase, and particularly, Ca(2+) exhibited remarkable improvement effect by 2...
November 15, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27853174/a-hyperaccumulation-pathway-to-three-dimensional-hierarchical-porous-nanocomposites-for-highly-robust-high-power-electrodes
#9
Jian Zhu, Yu Shan, Tao Wang, Hongtao Sun, Zipeng Zhao, Lin Mei, Zheng Fan, Zhi Xu, Imran Shakir, Yu Huang, Bingan Lu, Xiangfeng Duan
Natural plants consist of a hierarchical architecture featuring an intricate network of highly interconnected struts and channels that not only ensure extraordinary structural stability, but also allow efficient transport of nutrients and electrolytes throughout the entire plants. Here we show that a hyperaccumulation effect can allow efficient enrichment of selected metal ions (for example, Sn(2+), Mn(2+)) in the halophytic plants, which can then be converted into three-dimensional carbon/metal oxide (3DC/MOx) nanocomposites with both the composition and structure hierarchy...
November 17, 2016: Nature Communications
https://www.readbyqxmd.com/read/27829613/quantification-of-triacylglycerol-positional-isomers-in-rat-milk
#10
Natsuko Watanabe, Toshiharu Nagai, Hoyo Mizobe, Kazuaki Yoshinaga, Akihiko Yoshida, Yohei Kitamura, Takashi Shimizu, Fumiaki Beppu, Naohiro Gotoh
The absolute amount of triacylglycerol (TAG) positional isomers was analyzed in rat milk fat, a representative of non-ruminant milk fat, using a HPLC-UV-atmospheric pressure chemical ionization-MS/MS system equipped with an octacosyl silylation column or polymeric ODS column. TAGs consisting of two oleic acids (O) and one palmitic acid (P) were the most abundant. In particular, β-OPO, a TAG binding P at the β-position (sn-2) and two Os at the α-positions (sn-1/3), was prominent. The β-OPO content decreased over time, while a TAG consisting of two Ps and one capric acid, a medium-chain fatty acid, increased...
December 1, 2016: Journal of Oleo Science
https://www.readbyqxmd.com/read/27791304/lead-free-blue-emitting-bismuth-halide-perovskite-quantum-dots
#11
Meiying Leng, Zhengwu Chen, Ying Yang, Zha Li, Kai Zeng, Kanghua Li, Guangda Niu, Yisu He, Qingchao Zhou, Jiang Tang
Lead halide perovskite quantum dots (QDs) are promising candidates for future lighting applications, due to their high quantum yield, narrow full width at half maximum (FWHM), and wide color gamut. However, the toxicity of lead represents a potential obstacle to their utilization. Although tin(II) has been used to replace lead in films and QDs, the high intrinsic defect density and oxidation vulnerability typically leads to unsatisfactory material properties. Bismuth, with much lower toxicity than lead, is promising to constitute lead-free perovskite materials because Bi(3+) is isoelectronic to Pb(2+) and more stable than Sn(2+) ...
October 28, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27783700/characterisation-of-fecal-soap-fatty-acids-calcium-contents-bacterial-community-and-short-chain-fatty-acids-in-sprague-dawley-rats-fed-with-different-sn-2-palmitic-triacylglycerols-diets
#12
Jianchun Wan, Songyou Hu, Kefeng Ni, Guifang Chang, Xiangjun Sun, Liangli Yu
The structure of dietary triacylglycerols is thought to influence fatty acid and calcium absorption, as well as intestinal microbiota population of the host. In the present study, we investigated the impact of palmitic acid (PA) esterified at the sn-2 position on absorption of fatty acid and calcium and composition of intestinal microorganisms in rats fed high-fat diets containing either low sn-2 PA (12.1%), medium sn-2 PA (40.4%) or high sn-2 PA (56.3%), respectively. Fecal fatty acid profiles in the soaps were measured by gas chromatography (GC), while fecal calcium concentration was detected by ICP-MS...
2016: PloS One
https://www.readbyqxmd.com/read/27770698/competition-of-thermomyces-lanuginosus-lipase-with-its-hydrolysis-products-at-the-oil-water-interface
#13
Marco Muth, Stefanie Rothkötter, Steven Paprosch, Reiner P Schmid, Klaus Schnitzlein
Lipase-catalyzed hydrolysis of triglycerides yields glycerol and free fatty-acids, provided that the enzyme is non-regioselective. For an Sn-1,3 regioselective enzyme, such as lipase from Thermomyces lanuginosus, the final product is no longer glycerol but Sn-2 monoglyceride instead. However, surface active molecules generated by lipolysis may have a detrimental effect on the interfacial biocatalysis since it is known that low molecular weight surfactants can displace proteins from interfaces. By using drop profile analysis tensiometry, we evaluated the interfacial properties of the lipase-generated molecules and their competitive effect on the adsorption behavior of the lipase and on the proceeding lipolysis...
October 12, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27758859/cloning-of-glycerophosphocholine-acyltransferase-gpcat-from-fungi-and-plants-a-novel-enzyme-in-phosphatidylcholine-synthesis
#14
Bartosz Głąb, Mirela Beganovic, Sanket Anaokar, Meng-Shu Hao, Allan G Rasmusson, Jana Patton-Vogt, Antoni Banaś, Sten Stymne, Ida Lager
Glycero-3-phosphocholine (GPC), the product of the complete deacylation of phosphatidylcholine (PC), was long thought to not be a substrate for reacylation. However, it was recently shown that cell-free extracts from yeast and plants could acylate GPC with acyl groups from acyl-CoA. By screening enzyme activities of extracts derived from a yeast knock-out collection, we were able to identify and clone the yeast gene (GPC1) encoding the enzyme, named glycerophosphocholine acyltransferase (GPCAT). By homology search, we also identified and cloned GPCAT genes from three plant species...
November 25, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27757707/platelet-activating-factor-quantification-using-reversed-phase-liquid-chromatography-and-selected-reaction-monitoring-in-negative-ion-mode
#15
Daniel P Pike, Celine L Hartman, Gregory J Weissler, Elisa N D Palladino, Carolyn J Albert, David A Ford
Platelet-activating factor (PAF) is a potent biologically active phospholipid that mediates human physiological and pathophysiologic responses. PAF levels increase transiently and are typically assessed by techniques with limitations related to expense, sensitivity, pre-analysis derivatization and interference with isobaric molecules. This study elucidates a facile, accurate liquid chromatography-mass spectrometry analytical method for PAF. In negative ion mode using electrospray ionization, collisionally-activated dissociation analysis showed a unique product ion for acetate adducts of PAF molecular species representing the loss of methyl acetate from the polar head group and loss of a part of the acetate group from the sn-2 position...
December 2016: Lipids
https://www.readbyqxmd.com/read/27737316/impact-of-acute-undernutrition-on-growth-ileal-morphology-and-nutrient-transport-in-a-murine-model
#16
I C Sampaio, P H Q S Medeiros, F A P Rodrigues, P A Cavalcante, S A Ribeiro, J S Oliveira, M M G Prata, D V S Costa, S G C Fonseca, M M Guedes, A M Soares, G A C Brito, A Havt, S R Moore, A A M Lima
Undernutrition represents a major public health challenge for middle- and low-income countries. This study aimed to evaluate whether a multideficient Northeast Brazil regional basic diet (RBD) induces acute morphological and functional changes in the ileum of mice. Swiss mice (∼25 g) were allocated into two groups: i) control mice were fed a standard diet and II) undernourished mice were fed the RBD. After 7 days, mice were killed and the ileum collected for evaluation of electrophysiological parameters (Ussing chambers), transcription (RT-qPCR) and protein expression (western blotting) of intestinal transporters and tight junctions...
October 10, 2016: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
https://www.readbyqxmd.com/read/27712944/degradation-of-organic-pollutants-by-ag-cu-and-sn-doped-waste-non-metallic-printed-circuit-boards
#17
Kadari Ramaswamy, Velchuri Radha, M Malathi, Muga Vithal, Nagegownivari R Munirathnam
The disposal and reuse of waste printed circuit boards have been the major global concerns. Printed circuit boards, a form of Electronic waste (hereafter e-waste), have been chemically processed, doped with Ag(+), Cu(2+) and Sn(2+), and used as visible light photocatalysts against the degradation of methylene blue and methyl violet. The elemental analyses of pristine and metal doped printed circuit board were obtained using energy dispersive X-ray fluorescence (EDXRF) spectra and inductively coupled plasma optical emission spectroscopy (ICP-OES)...
October 3, 2016: Waste Management
https://www.readbyqxmd.com/read/27711801/plasmon-resonance-energy-transfer-and-hot-electron-injection-induced-high-photocurrent-density-in-liquid-junction-ag-ag2s-sensitized-solar-cells
#18
Dapeng Wu, Fujuan Wang, Hongju Wang, Kun Cao, Zhiyong Gao, Fang Xu, Kai Jiang
An in situ technique was developed to deposit Ag@Ag2S core-shell quantum dots on a SnO2 mesoporous film for solar energy conversion. When adopted as a photoanode, an impressive high photocurrent density of ∼25.6 mA cm(-2) was demonstrated in a cell configuration using polysulfide S(2-)/Sn(2-) as an electrolyte and Cu2S/brass as a counter electrode, which leads to a power conversion efficiency of ∼0.784% for this environmentally benign device. Optical measurements showed that Ag nanoparticles could be employed as plasmon resonance centers to enhance the harvesting efficiency of incident light at the visible and near-infrared range...
October 18, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27688258/quantitation-of-isobaric-phosphatidylcholine-species-in-human-plasma-using-a-hybrid-quadrupole-linear-ion-trap-mass-spectrometer
#19
Petr Zacek, Michael Bukowski, Thad A Rosenberger, Matthew Picklo
Phosphatidylcholine (PC) species in human plasma are used as biomarkers of disease. PC biomarkers are often limited by the inability to separate isobaric PCs. In this work, we developed a targeted shotgun approach for analysis of isobaric and isomeric PCs. This approach is comprised of two MS methods: a precursor ion scanning (PIS) of mass m/z 184 in positive mode (PIS m/z +184) and MS(3) fragmentation in negative mode, both performed on the same instrument, a hybrid triple quadrupole ion-trap mass spectrometer...
December 2016: Journal of Lipid Research
https://www.readbyqxmd.com/read/27651104/dynamical-bifurcation-in-gas-phase-xh-ch3-y-sn-2-reactions-the-role-of-energy-flow-and-redistribution-in-avoiding-the-minimum-energy-path
#20
Yaicel G Proenza, Miguel A F de Souza, Ricardo L Longo
The gas-phase reactions of XH(-) (X=O, S) + CH3 Y (Y=F, Cl, Br) span nearly the whole range of SN 2 pathways, and show an intrinsic reaction coordinate (IRC) (minimum energy path) with a deep well owing to the CH3 XH⋅⋅⋅Y(-) (or CH3 S(-) ⋅⋅⋅HF) hydrogen-bonded postreaction complex. MP2 quasiclassical-type direct dynamics starting at the [HX⋅⋅⋅CH3 ⋅⋅⋅Y](-) transition-state (TS) structure reveal distinct mechanistic behaviors. Trajectories that yield the separated CH3 XH+Y(-) (or CH3 S(-) +HF) products directly are non-IRC, whereas those that sample the CH3 XH⋅⋅⋅Y(-) (or CH3 S(-) ⋅⋅⋅HF) complex are IRC...
November 2, 2016: Chemistry: a European Journal
keyword
keyword
69012
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"