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Farzad Pakdel

Denis Habauzit, Catherine Martin, Gwenneg Kerdivel, Farzad Pakdel
CXCL-test is a method that uses the estrogen-dependent secretion of the natural endogenous chemokine CXCL12 to evaluate the estrogenic activity of molecules. CXCL12 chemokine is involved in the estrogen dependent proliferation of breast cancer cells. Its measure is an indicator of cell proliferation and is used as an alternative test to classical proliferation test. Here we aimed to optimize this test, first to increase the number of tested molecules in a single assay and then to decrease the number of intermediate steps...
January 6, 2017: Chemosphere
Mohsen Bahmani Kashkouli, Mohammad Bagher Abtahi, Hamed Sianati, Nina Mahvidizadeh, Farzad Pakdel, Pedram Bahmani Kashkouli, Parya Abdolalizadeh
PURPOSE: To report 10-year results of a 1-stage, obstruction-based, endoscopic approach in children with congenital nasolacrimal duct obstruction (CNLDO) with and without prior failed probing/intubation. METHODS: In a retrospective study, children with primary CNLDO of >6 months old previously failed probing/intubation, acute dacryocystitis or dacryocele at any age, and at least 6 months follow up. Diagnosis was based on history of tearing and dye disappearance test...
September 15, 2016: Ophthalmic Plastic and Reconstructive Surgery
Joel Cano-Nicolau, Clémentine Garoche, Nathalie Hinfray, Elisabeth Pellegrini, Noureddine Boujrad, Farzad Pakdel, Olivier Kah, François Brion
The effects of some progestins on fish reproduction have been recently reported revealing the hazard of this class of steroidal pharmaceuticals. However, their effects at the central nervous system level have been poorly studied until now. Notwithstanding, progesterone, although still widely considered primarily a sex hormone, is an important agent affecting many central nervous system functions. Herein, we investigated the effects of a large set of synthetic ligands of the nuclear progesterone receptor on the glial-specific expression of the zebrafish brain aromatase (cyp19a1b) using zebrafish mechanism-based assays...
August 15, 2016: Toxicology and Applied Pharmacology
Lina Chouchene, Elisabeth Pellegrini, Marie-Madeleine Gueguen, Nathalie Hinfray, François Brion, Benjamin Piccini, Olivier Kah, Khaled Saïd, Imed Messaoudi, Farzad Pakdel
The present study was conducted to assess the effects of Cd exposure on estrogen signaling in the zebrafish brain, as well as the potential protective role of Zn against Cd-induced toxicity. For this purpose, the effects on transcriptional activation of the estrogen receptors (ERs), aromatase B (Aro-B) protein expression and molecular expression of related genes were examined in vivo using wild-type and transgenic zebrafish embryos. For in vitro studies, an ER-negative glial cell line (U251MG) transfected with different zebrafish ER subtypes (ERα, ERβ1 and ERβ2) was also used...
June 2016: Journal of Applied Toxicology: JAT
Farzad Pakdel, Homayoun Hadizadeh, Niloofar Pirmarzdashty, Victoria Kiavash
A 59-year-old patient developed acute proptosis, peri-orbital swelling and restriction of ocular movements 2 days after attempted scleral buckle removal. Initial clinical and orbital MRI findings were suggestive for orbital cellulitis and orbital abscess. Empiric intravenous antibiotics were not effective. Proton magnetic resonance spectroscopy (MRS) revealed a distinctive composition and helped rule out suppurative and neoplastic processes. The patient recovered soon after removing clear liquefied and tiny particles of the hydrogel buckle by an effective peristaltic technique...
2015: Orbit
Caroline Pinto, Marina Grimaldi, Abdelhay Boulahtouf, Farzad Pakdel, François Brion, Sélim Aït-Aïssa, Vincent Cavaillès, William Bourguet, Jan-Ake Gustafsson, Maria Bondesson, Patrick Balaguer
Zebrafish, Danio rerio, is increasingly used as an animal model to study the effects of pharmaceuticals and environmental estrogens. As most of these estrogens have only been tested on human estrogen receptors (ERs), it is necessary to measure their effects on zebrafish ERs. In humans there are two distinct nuclear ERs (hERα and hERβ), whereas the zebrafish genome encodes three ERs, zfERα and two zfERβs (zfERβ1 and zfERβ2). In this study, we established HeLa-based reporter cell lines stably expressing each of the three zfERs...
October 1, 2014: Toxicology and Applied Pharmacology
Denis Habauzit, François Ferrière, Nadine Botherel, Gilles Flouriot, Farzad Pakdel, Christian Saligaut
Xeno-estrogens, a class of endocrine disrupting chemicals (EDCs), can disturb estrogen receptor-dependent pathways involved in differentiation, proliferation or protection. Multiple methods have been developed to characterize the disturbances induced by EDCs in different cells or organs. In this study we have developed a new tool for the assessment of estrogenic compounds on differentiation. For this purpose we used the global model of NGF-induced neurite outgrowth of a pseudoneuronal PC12 cell line stably transfected with estrogen receptor alpha (PC12 ER)...
October 2014: Chemosphere
Antoine Boudot, Gwenneg Kerdivel, Sylvain Lecomte, Gilles Flouriot, Mireille Desille, Florence Godey, Jean Leveque, Patrick Tas, Yves Le Dréan, Farzad Pakdel
BACKGROUND: The orphan receptors COUP-TF (chicken ovalbumin upstream promoter transcription factor) I and II are members of the nuclear receptor superfamily that play distinct and critical roles in vertebrate organogenesis. The involvement of COUP-TFs in cancer development has recently been suggested by several studies but remains poorly understood. METHODS: MCF-7 breast cancer cells overexpressing COUP-TFI and human breast tumors were used to investigate the role of COUP-TFI in the regulation of CXCL12/CXCR4 signaling axis in relation to cell growth and migration...
2014: BMC Cancer
Gilles Flouriot, Guillaume Huet, Florence Demay, Farzad Pakdel, Noureddine Boujrad, Denis Michel
In addition to soluble factors, mechanical constraints and extracellular matrix stiffness are important regulators of cell fate that are mediated by cytoskeletal modifications. The EMT (epithelial-mesenchymal transition) that occurs during normal development and malignant progression is a typical example of the phenotypic switch associated with profound actin remodelling and changes in gene expression. For instance, actin dynamics control motile cell functions in EMT, in part, through regulating the subcellular localization of the myocardin-related transcription factor MKL1 (megakaryoblastic leukaemia translocation 1), a co-activator of SRF (serum-responsive factor)...
July 15, 2014: Biochemical Journal
Gwenneg Kerdivel, Antoine Boudot, Denis Habauzit, Frederic Percevault, Florence Demay, Farzad Pakdel, Gilles Flouriot
Estrogen receptor alpha (ERα) is generally considered to be a good prognostic marker because almost 70% of ERα-positive tumors respond to anti-hormone therapies. Unfortunately, during cancer progression, mammary tumors can escape from estrogen control, resulting in resistance to treatment. In this study, we demonstrate that activation of the actin/megakaryoblastic leukemia 1 (MKL1) signaling pathway promotes the hormonal escape of estrogen-sensitive breast cancer cell lines. The actin/MKL1 signaling pathway is silenced in differentiated ERα-positive breast cancer MCF-7 and T47D cell lines and active in ERα-negative HMT-3522 T4-2 and MDA-MB-231 breast cancer cells, which have undergone epithelial-mesenchymal transition...
June 5, 2014: Molecular and Cellular Endocrinology
Antoine Boudot, François Le Dily, Farzad Pakdel
The orphan receptors COUP-TFI and COUP-TFII are members of the nuclear receptor superfamily that play distinct and critical roles in vertebrate organogenesis, as demonstrated by loss-of-function COUP-TFI and/or COUP-TFII mutant mice. Although COUP-TFs are expressed in a wide range of tissues in adults, little is known about their functions at later stages of development or in organism homeostasis. COUP-TFs are expressed in cancer cell lines of various origins and increasing studies suggest they play roles in cell fate determination and, potentially, in cancer progression...
2011: Cancers
François Ferriere, Denis Habauzit, Farzad Pakdel, Christian Saligaut, Gilles Flouriot
Many studies have reported proliferative, differentiating or protective effects of estradiol, notably through estrogen receptor alpha (ERα). On the contrary, the ligand-independent action of ERα is currently poorly documented notably in cell protection. The stable transfection of wild type, substituted or truncated form of ERα in PC12 cells (ERα negative cell line) lead the specific study of its ligand-independent action. Hence, we demonstrate here that, in the absence of E2, the expression of ERα prevents cells from apoptosis induced by serum deprivation...
2013: PloS One
Gwenneg Kerdivel, Gilles Flouriot, Farzad Pakdel
Seventy percent of breast tumors express the estrogen receptor (ER), which is generally considered to predict a better outcome relative to ER-negative tumors, as they often respond to antiestrogen therapies. During cancer progression, mammary tumors can escape from estrogen control, resulting in the acquisition of invasive properties and resistance to treatment. ER expression is a dynamic phenomenon and is finely regulated at numerous levels, including the gene, mRNA, and protein levels. As a consequence, many molecular mechanisms have been implicated in modulating ER activity and estrogen signaling in mammary cancer...
2013: Vitamins and Hormones
Gwenneg Kerdivel, Denis Habauzit, Farzad Pakdel
In all vertebrate species, estrogens play a crucial role in the development, growth, and function of reproductive and nonreproductive tissues. A large number of natural or synthetic chemicals present in the environment and diet can interfere with estrogen signaling; these chemicals are called endocrine disrupting chemicals (EDCs) or xenoestrogens. Some of these compounds have been shown to induce adverse effects on human and animal health, and some compounds are suspected to contribute to diverse disease development...
2013: International Journal of Endocrinology
Mohsen Bahmani Kashkouli, Farzad Pakdel, Victoria Kiavash, Leila Ghiasian, Abtin Heirati, Mansooreh Jamshidian-Tehrani
PURPOSE: To assess the results and satisfaction after transconjunctival lower blepharoplasty, evaluate the effect of associated aesthetic procedures on the satisfaction, and compare surgeon versus subjects' perspective on the results. METHODS: In a retrospective cross-sectional study, a chart review was performed on transconjunctival lower blepharoplasty procedures performed by 1 eye plastic surgeon from April 2003 to April 2009. Subjects with less than 6 months follow-up time or reoperation were excluded...
July 2013: Ophthalmic Plastic and Reconstructive Surgery
Gwenneg Kerdivel, Remy Le Guevel, Denis Habauzit, François Brion, Selim Ait-Aissa, Farzad Pakdel
The results from recent studies show that some benzophenones (BPs) and their hydroxylated metabolites can function as weak estrogens (E2) in the environment. However, little is known about the structure-activity relationship of these molecules. We have examined the effects of exposure to ten different BPs on the proliferation of estrogen receptor (ER)-positive breast cancer cells and on the transcriptional activity of E2-target genes. We analyzed two genes that are tightly linked with estrogen-mediated proliferation, the CXCL12 and amphiregulin genes and two classical estrogen-responsive genes, the pS2 and progesterone receptor...
2013: PloS One
Mohsen Bahmani Kashkouli, Hoora Mirzajani, Mansooreh Jamshidian-Tehrani, Farzad Pakdel, Marzieh Nojomi, Gholam-Hossein Aghaei
PURPOSE: : To evaluate the role of fluorescein dye disappearance test (FDDT) in assessment of adults with primary acquired nasolacrimal duct obstruction (PANDO) and its correlation with age and severity of epiphora. METHODS: : In a prospective, cross-sectional comparative study, FDDT at 2 (2-FDDT), 5 (5-FDDT), and 10 (10-FDDT) minutes were performed in 58 eyes of 58 patients with PANDO (case) and 58 eyes of 58 subjects without epiphora (control) between 2008 and 2010...
May 2013: Ophthalmic Plastic and Reconstructive Surgery
Gwenneg Kerdivel, Antoine Boudot, Farzad Pakdel
Although many studies reported mechanisms involved in the positive regulation of estrogens (E2) target genes, very little is known concerning the repressive effect of E2. In this study, we explored the molecular mechanisms by which E2 regulates CXCR7 expression in breast cancer cells. Our results show that E2-mediated down-regulation of CXCR7 occurs at the transcriptional level as demonstrated using actinomycin D and requires estrogen receptor alpha (ERα). In addition, CXCR7 is a primary ERα-target gene because the effect of E2 does not require the synthesis of an intermediary protein as revealed by the translational inhibitor cycloheximide treatment...
February 22, 2013: Biochemical and Biophysical Research Communications
Farzad Pakdel
No abstract text is available yet for this article.
July 2012: Journal of Ophthalmic & Vision Research
Farzad Pakdel, Mohsen Bahmani Kashkouli
No abstract text is available yet for this article.
October 2009: Journal of Ophthalmic & Vision Research
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