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https://www.readbyqxmd.com/read/27915385/substantial-variation-among-hernia-experts-in-the-decision-for-treatment-of-patients-with-incisional-hernia-a-descriptive-study-on-agreement
#1
D Kokotovic, I Gögenur, F Helgstrand
PURPOSE: Benign elective procedures give rise to heterogeneity in indication for surgery and surgical technique among specialized surgeons in a variety of surgical fields. The objective was to analyze the extent of agreement in surgical management among expert hernia surgeons when evaluating the same patient in a standardized setting. METHODS: Five Danish hernia experts answered questions concerning indication for surgery and surgical technique for 25 video recorded real-life clinical cases...
December 3, 2016: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/27915198/a-new-approach-to-the-rapid-separation-of-isomeric-compounds-in-a-silybum-marianum-extract-using-uhplc-core-shell-column-with-f5-stationary-phase
#2
Jakub Fibigr, Dalibor Šatínský, Petr Solich
In this paper, a new ultra-high performance liquid chromatography (UHPLC) method using a core-shell column with a pentafluorophenyl stationary phase for separation of seven active compounds of a Silybum marianum extract was developed and validated. Silymarin, an extract of Silybum marianum, is known for its abilities to protect the liver from toxic substances, hepatitis therapy, and anti-tumour activity. Silymarin is currently being widely used in commercial preparations and herbal teas. Separation of seven compounds contained in the Silybum marianum extract (taxifolin, silychristin, silydianin, silybin A, silybin B, isosilybin A, isosilybin B) and other substances occurring in real samples was performed on the Kinetex 1...
November 27, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27915188/combined-cluster-and-discriminant-analysis-an-efficient-chemometric-approach-in-diesel-fuel-characterization
#3
Márton Novák, Dóra Palya, Zsolt Bodai, Zoltán Nyiri, Norbert Magyar, József Kovács, Zsuzsanna Eke
Combined cluster and discriminant analysis (CCDA) as a chemometric tool in compound specific isotope analysis of diesel fuels was studied. The stable carbon isotope ratios (δ(13)C) of n-alkanes in diesel fuel can be used to characterize or differentiate diesels originating from different sources. We investigated 25 diesel fuel samples representing 20 different brands. The samples were collected from 25 different service stations in 11 European countries over a 2 year period. The n-alkane fraction of diesel fuels was separated using solid-state urea clathrate formation combined with silica gel fractionation...
November 23, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27914378/toward-ocean-attenuation-tomography-determining-acoustic-volume-attenuation-coefficients-in-seawater-using-eigenray-amplitudes
#4
J D Tippmann, J Sarkar, C M A Verlinden, W S Hodgkiss, W A Kuperman
A deep-water experiment in the Pacific made in situ measurements of the volume attenuation coefficients of sea water in the mid-frequency range. The frequency, temperature, salinity, pH, and pressure dependent seawater attenuation coefficients were determined using a vertical line array that received and identified over 2000 unique paths from 1200 mid-frequency 3-9 kHz LFM source transmissions at a convergence zone range and depth up to 400 m. The results show no change in attenuation coefficients in this band compared to estimates from 30-year-old models previously determined from a combination of long-range ocean acoustic and laboratory experiments...
September 2016: Journal of the Acoustical Society of America
https://www.readbyqxmd.com/read/27914015/electron-transfer-dissociation-of-all-ions-at-all-times-ms-etd-in-a-quadrupole-time-of-flight-q-tof-mass-spectrometer
#5
Christian N Cramer, Jeffery M Brown, Nick Tomczyk, Peter Kresten Nielsen, Kim F Haselmann
Data-independent mass spectral acquisition is particularly powerful when combined with ultra-performance liquid chromatography (LC) that provides excellent separation of most components present in a given sample. Data-independent analysis (DIA) consists of alternating full MS scans and scans with fragmentation of all ions within a selected m/z range, providing precursor masses and structure information, respectively. Fragmentation spectra are acquired either by sequential isolation and fragmentation of sliding m/z ranges or fragmenting all ions entering the MS instrument with no ion isolation, termed broadband DIA...
December 2, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27913618/meiotic-centromere-coupling-and-pairing-function-by-two-separate-mechanisms-in-saccharomyces-cerevisiae
#6
Emily L Kurdzo, David Obeso, Hoa Chuong, Dean S Dawson
In meiosis I, chromosomes become paired with their homologous partners and then are pulled towards opposite poles of the spindle. In the budding yeast, Saccharomyces cerevisiae, in early meiotic prophase, centromeres are observed to associate in pairs in a homology-independent manner, a process called centromere coupling. Later, as homologous chromosomes align, their centromeres associate in a process called centromere pairing. The synaptonemal complex protein Zip1 is necessary for both types of centromere association...
December 2, 2016: Genetics
https://www.readbyqxmd.com/read/27913377/optimization-and-assessment-of-three-different-high-performance-liquid-chromatographic-systems-for-the-combinative-fingerprint-analysis-and-multi-ingredients-quantification-of-sangju-ganmao-tablet
#7
Meng-Zhe Guo, Jie Han, Dan-Dan He, Jia-Hui Zou, Zheng Li, Yan Du, Dao-Quan Tang
Chromatographic separation is still a critical subject for the quality control of traditional Chinese medicine. In this study, three different high performance liquid chromatographic (HPLC) systems employing commercially available columns packed with 1.8, 3.5 and 5.0 μm particles were respectively developed and optimized for the combinative fingerprint analysis and multi-ingredients quantification of Sangju Ganmao tablet (SGT). Chromatographic parameters including the repeatability of retention time and peak area, symmetry factor, resolution, number of theoretical plates and peak capacity were used to assess the chromatographic performance of different HPLC systems...
December 1, 2016: Journal of Chromatographic Science
https://www.readbyqxmd.com/read/27913361/constrained-null-space-component-analysis-for-semiblind-source-separation-problem
#8
Wen-Liang Hwang, Keng-Shih Lu, Jinn Ho
The blind source separation (BSS) problem extracts unknown sources from observations of their unknown mixtures. A current trend in BSS is the semiblind approach, which incorporates prior information on sources or how the sources are mixed. The constrained independent component analysis (ICA) approach has been studied to impose constraints on the famous ICA framework. We introduced an alternative approach based on the null space component (NCA) framework and referred to the approach as the c-NCA approach. We also presented the c-NCA algorithm that uses signal-dependent semidefinite operators, which is a bilinear mapping, as signatures for operator design in the c-NCA approach...
November 29, 2016: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/27913217/integration-of-whole-cell-reaction-and-product-isolation-highly-hydrophobic-solvents-promote-in-situ-substrate-supply-and-simplify-extractive-product-isolation
#9
Dorothea Leis, Bernhard Lauß, Robert Macher-Ambrosch, Andreas Pfennig, Bernd Nidetzky, Regina Kratzer
Product isolation from aqueous-organic reaction mixtures that contain high concentrations of whole cells constitutes a challenging task in bioprocessing. Stirring of the biphasic reaction media leads to the formation of solvent droplets coated by surface active components and an emulsion forms. We used an early focus on phase separation to simplify a whole-cell bioreduction. Octanol, heptanol, hexanol, hexane and dipropylether were tested as co-solvents in the E. coli catalyzed reduction of o-chloroacetophenone...
November 29, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27913156/the-oral-spelling-profile-of-posterior-cortical-atrophy-and-the-nature-of-the-graphemic-representation
#10
Silvia Primativo, Keir X X Yong, Timothy J Shakespeare, Sebastian J Crutch
Spelling is a complex cognitive task where central and peripheral components are involved in engaging resources from many different cognitive processes. The present paper aims to both characterize the oral spelling deficit in a population of patients affected by a neurodegenerative condition and to clarify the nature of the graphemic representation within the currently available spelling models. Indeed, the nature of graphemic representation as a linear or multi-componential structure is still debated. Different hypotheses have been raised about its nature in the orthographic lexicon, with one positing that graphemes are complex objects whereby quantity and identity are separately represented in orthographic representations and can thus be selectively impaired...
November 29, 2016: Neuropsychologia
https://www.readbyqxmd.com/read/27913143/innate-immune-stimulatory-activity-of-porphyromonas-gingivalis-fimbriae-is-eliminated-by-phase-separation-using-triton-x-114
#11
Kohji Nozoe, Terukazu Sanui, Masaaki Takeshita, Takao Fukuda, Akira Haraguchi, Yoshitomi Aida, Fusanori Nishimura
Fimbriae are virulence factors of Porphyromonas gingivalis (P. gingivalis). In this study, the action of fimbriae on neutrophil respiratory burst and cytokine production by mononuclear cells (MNC) were investigated. Native or denatured form of purified P. gingivalis fimbriae contained endotoxin at an equivalence of 1-3μglipopolysaccharides(LPS)/mg protein. The endotoxin could be reduced to the equivalent of 1ng-LPS/mg protein by phase separation using Triton X-114. Unfractionated fimbriae caused serum-dependent priming of neutrophils for enhanced respiratory burst, but both native and denatured forms of Triton X-114-fractionated fimbriae were not active at 100μg/mL...
November 30, 2016: Journal of Immunological Methods
https://www.readbyqxmd.com/read/27913102/membrane-permeabilization-induced-by-triton-x-100-the-role-of-membrane-phase-state-and-edge-tension
#12
Bruno Mattei, Rafael B Lira, Katia R Perez, Karin A Riske
Detergents are widely used to solubilize and separate biomembrane components. It is therefore relevant to study and understand the mechanistic details underlying detergent-lipid interactions using biomimetic systems. Here, we have investigated in detail the process of membrane permeabilization and the nature of pores induced by sub-solubilizing concentrations of the detergent Triton X-100 (TX-100) in bilayers composed of palmitoyl oleoyl phosphatidylcholine (POPC), sphingomyelin (SM) and binary mixtures of these phospholipids with 30 mol% cholesterol (chol)...
November 29, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27912918/the-use-of-potassium-hydroxide-koh-solution-as-a-suitable-approach-to-isolate-plastics-ingested-by-marine-organisms
#13
Susanne Kühn, Bernike van Werven, Albert van Oyen, André Meijboom, Elisa L Bravo Rebolledo, Jan A van Franeker
In studies of plastic ingestion by marine wildlife, visual separation of plastic particles from gastrointestinal tracts or their dietary content can be challenging. Earlier studies have used solutions to dissolve organic materials leaving synthetic particles unaffected. However, insufficient tests have been conducted to ensure that different categories of consumer products partly degraded in the environment and/or in gastrointestinal tracts were not affected. In this study 63 synthetic materials and 11 other dietary items and non-plastic marine debris were tested...
November 29, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27911555/electromagnetic-signatures-of-the-chiral-anomaly-in-weyl-semimetals
#14
Edwin Barnes, J J Heremans, Djordje Minic
Weyl semimetals are predicted to realize the three-dimensional axial anomaly first discussed in particle physics. The anomaly leads to unusual transport phenomena such as the chiral magnetic effect in which an applied magnetic field induces a current parallel to the field. Here we investigate diagnostics of the axial anomaly based on the fundamental equations of axion electrodynamics. We find that materials with Weyl nodes of opposite chirality and finite energy separation immersed in a uniform magnetic field exhibit an anomaly-induced oscillatory magnetic field with a period set by the chemical potential difference of the nodes...
November 18, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27910774/comparison-of-the-chemical-composition-and-bioactive-components-of-fruiting-bodies-and-submerged-cultured-mycelia-of-the-willow-bracket-medicinal-mushroom-phellinus-igniarius-agaricomycetes
#15
Huawei Zeng, Wenfeng Wang, Menghua Ma, Guohua Xu, Mei Ying-Jie, Jianfan Sund
Chemical compositions and bioactive ingredients of dried fruiting bodies from Phellinus igniarius (CGMCC no. 50095) (P1) and submerged culture of Ph. igniarius dried mycelia (P2) were investigated in this study. It was found that glutamic acid was regarded as a major amino acid in P1 (1.20%) and was approximately 2.55-fold higher than that in P2 (0.47%). Total amino acids in P1 (5.36%) were slightly higher than in P2 (4.09%). The amounts of iron, zinc, copper, and manganese in P1 were 1.96-3.42 times as high as those in P2, whereas potassium, sodium, and magnesium in P2 were almost 2...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27910757/free-radical-scavenging-activities-of-cultured-mycelia-of-paecilomyces-hepiali-ascomycetes-extracts-and-structural-characterization-of-bioactive-components-by-nuclear-magnetic-resonance-spectroscopy
#16
Lucia Ungvarska Mal'ucka, Jarmila Harvanova, Martin Pavlik, Martin Rajtar, Lukas Jarosciak
Current research is focused on testing the cultivation of Paecilomyces hepiali mycelia on various plant substrates and producing fungus or mycelial biomass with qualitatively interesting substances. P. hepiali mycelia was cultivated using solid-state fermentation of different substrates. Mycelial biomass was then analyzed, and antioxidant activity was evaluated using the DPPH radical scavenging method for different ethanolic extracts based on a millet substrate (extract 1) or a chickpea substrate (extract 2)...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27909777/quantitative-determination-of-the-neurotoxin-%C3%AE-n-methylamino-l-alanine-bmaa-by-capillary-electrophoresis-tandem-mass-spectrometry
#17
Elliott S Kerrin, Robert L White, Michael A Quilliam
Recent reports of the widespread occurrence of the neurotoxin β-N-methylamino-L-alanine (BMAA) in cyanobacteria and particularly seafood have raised concerns for public health. LC-MS/MS is currently the analytical method of choice for BMAA determinations but incomplete separation of isomeric and isobaric compounds, matrix suppression and conjugated forms are plausible limitations. In this study, capillary electrophoresis (CE) coupled with MS/MS has been developed as an alternative method for the quantitative determination of free BMAA...
December 1, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27908507/usage-of-air-jigging-for-multi-component-separation-of-construction-and-demolition-waste
#18
Weslei Monteiro Ambrós, Carlos Hoffmann Sampaio, Bogdan Grigore Cazacliu, Gerson Luis Miltzarek, Leonardo R Miranda
The use of air jigging for performing multi-component separation in the treatment of mixed construction and demolition waste was studied. Sorting tests were carried out with mixtures of equal bulk volume of concrete and brick in which fixed quantities of unwanted materials - gypsum, wood and paper - were added. Experimental results have demonstrated the possibility to use air jigging to carry out both the removal of low-density contaminants and the concrete concentration in only one process step. In relation to the removal of contaminants only, the overall performance of jigging process can be comparable with that of commercial air classifiers and automatic sorting systems...
November 28, 2016: Waste Management
https://www.readbyqxmd.com/read/27908499/a-strategy-to-modulate-the-electrophoretic-behavior-in-plastic-microchips-using-sodium-polystyrene-sulfonate
#19
Jinxiu Guo, Yu Chen, Lizhi Zhao, Ping Sun, Hongli Li, Lei Zhou, Xiayan Wang, Qiaosheng Pu
Plastic microchips have been broadly used as disposable microfluidic devices, but the poorly defined surface properties limit their application. Herein, we proved that an anionic polymer could be used as the background electrolyte (BGE) to provide a strong and stable cathodic electroosmotic flow (EOF) and modulate the electrophoretic behavior for efficient separation in relative thicker microchannels (∼75μm id). A cathodic EOF of ∼3.3×10(-4)cm(2)V(-1)s(-1) was maintained using sodium polystyrene sulfonate (PSSNa) with a molecular weight of 5×10(5) as the BGE, which ensured fluorescein isothiocyanate labeled biogenic amines (BAs) appeared ahead of other components in the electropherograms obtained with microchips of cyclic olefin copolymer...
November 23, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/27908140/sequence-control-of-phase-separation-and-dewetting-in-ps-pvme-blend-thin-films-by-changing-molecular-weight-of-ps
#20
Tian Xia, Yaping Qin, Yajiang Huang, Ting Huang, Jianhui Xu, Youbing Li
The morphology evolution mechanism of polystyrene (PS)/poly (vinyl methyl ether) (PVME) blend thin films with different PS molecular weights (Mw) was studied. It was found that the morphology evolution was closely related to the molecular weight asymmetry between PS and PVME. In the film where Mw(PS) ≈ Mw(PVME), dewetting happened at the interface between the bottom layer and substrate after SD phase separation. While in the film where Mw(PS) > Mw(PVME), dewetting happened at the interface between the middle PS/PVME blend layer and bottom PVME layer near the substrate prior to phase separation...
November 28, 2016: Journal of Chemical Physics
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