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corneoscleral graft

Johannes Menzel-Severing, Friedrich E Kruse, Theofilos Tourtas
AIM: To determine whether clinical performance is negatively affected by prestripping Descemet membrane endothelial keratoplasty (DMEK) grafts from organ-cultured corneas. METHODS: We reviewed clinical records of all patients who underwent DMEK surgery for Fuchs endothelial dystrophy between 28 October 2014 and 11 August 2015. Grafts had been prepared from organ-cultured corneoscleral buttons 24 hours prior to surgery or during surgery. We included only patients for which at least one follow-up examination was available at a minimum of 2 months postoperatively...
December 14, 2016: British Journal of Ophthalmology
C Larena Menant-Tay, Ronan Conlon, Salina Teja, Season Yeung, Joshua Teichman, Setareh Ziai, Kashif Baig
OBJECTIVE: To assess the intraoperative issues and surgical outcomes of preparing a single-donor corneal tissue for same-day use in both deep anterior lamellar keratoplasty (DALK) and Descemet's membrane endothelial keratoplasty (DMEK). DESIGN: Consecutive retrospective case series. PARTICIPANTS: Ten eyes of 10 patients who underwent DALK (5 patients) or DMEK (5 patients) surgery using dual-purpose corneal tissue. METHODS: Five dual-purpose corneoscleral rims were used to prepare tissue for 5 DMEK and 5 DALK procedures...
December 2016: Canadian Journal of Ophthalmology. Journal Canadien D'ophtalmologie
Marco Lombardo, Mohit Parekh, Sebastiano Serrao, Alessandro Ruzza, Stefano Ferrari, Giuseppe Lombardo
PURPOSE: To investigate the microstructure of endothelial keratoplasty grafts using two-photon optical microscopy. METHODS: Six endothelial keratoplasty grafts obtained from human donor corneoscleral tissues and prepared by submerged hydrodissection technique were imaged by two-photon optical microscopy. In each graft, two liquid bubbles were created in order to investigate the presence of a conserved cleavage plane regardless of the volume of posterior stroma that remained attached to Descemet's membrane (DM); the first bubble (bubble A) was generated under DM and the second bubble (bubble B) injection was done in order to obtain a layer of deep stroma that kept the two bubbles separated...
November 17, 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
Jesse M Vislisel, Kenneth M Goins, Michael D Wagoner, Gregory A Schmidt, Benjamin T Aldrich, Jessica M Skeie, Cynthia R Reed, M Bridget Zimmerman, Mark A Greiner
PURPOSE: To determine the incidence of positive corneoscleral donor rim fungal cultures after keratoplasty and to report clinical outcomes of grafts with culture-positive donor rims. DESIGN: Retrospective cohort study. PARTICIPANTS: Consecutive donor corneas and keratoplasty recipients at a single tertiary referral center over 20 years. METHODS: Patient charts were reviewed to determine the incidence of positive donor rim fungal cultures and clinical outcomes of all grafts using contaminated tissue...
January 2017: Ophthalmology
Itay Lavy, Robert M Verdijk, Marieke Bruinsma, Hein F Sleddens, Silke Oellerich, Perry S Binder, Gerrit R J Melles
PURPOSE: To identify the origin of corneal endothelial cells (host or donor) present on grafts at various time points after Descemet membrane endothelial keratoplasty (DMEK), using fluorescence in situ hybridization (FISH) of sex chromosomes on post mortem corneas with sex mismatch between the donor and host. METHODS: Corneoscleral buttons of 6 post mortem DMEK eyes of 4 patients, operated for Fuchs endothelial dystrophy, with an average postoperative time of 2...
January 2017: Cornea
Kuan-I Wu, Hsiao-Sang Chu, Amy Shih-I Pai, Yu-Chih Hou, Szu-Yuan Lin, Wei-Li Chen, Fung-Rong Hu
PURPOSE: To assess the surgical and clinical outcomes of anterior lamellar keratoplasty using anterior corneal buttons from Descemet stripping automated endothelial keratoplasty (DSAEK) donor tissue. METHODS: Retrospective data from 8 patients with unilateral limbal dermoids, treated between February 2011 and January 2016 at National Taiwan University Hospital, were analyzed. Donor corneas for DSAEK were divided into anterior and posterior lamellae using a 350-μm microkeratome...
January 2017: Cornea
Esther A Groeneveld-van Beek, Jack Parker, Jessica T Lie, Vincent Bourgonje, Lisanne Ham, Korine van Dijk, Jacqueline van der Wees, Gerrit R J Melles
PURPOSE: To describe and compare 2 preparation techniques for Bowman layer (BL) grafts for use in BL transplantation. METHODS: A retrospective evaluation of the 2 methods for preparing BL grafts was performed, that is, BL graft preparation from donor globes not eligible for penetrating keratoplasty or endothelial keratoplasty (technique I, n = 36) and BL graft preparation from previously excised corneoscleral buttons (technique II, n = 36) that could not be used for PK or had been denuded of Descemet membrane and endothelium for Descemet membrane endothelial keratoplasty graft preparation...
June 29, 2016: Cornea
Andrew Xingyu Zhou, Balamurali Krishna Ambati
A 2-year-old boy with Goldenhar syndrome had a limbal dermoid removed and covered with a lamellar corneoscleral patch graft that was attached with fibrin glue and no sutures. The graft healed and attached well. A sutureless technique is beneficial due to decreased scarring and chance of infection.
June 3, 2016: Journal of Pediatric Ophthalmology and Strabismus
Dong Hyun Kim, Jaeyoung Kim, Hyun Jeong Jeong, Hyun Ju Lee, Mee Kum Kim, Won Ryang Wee
BACKGROUND: Xenocorneal transplantation is one of the solutions for shortage of donor cornea, and remarkable advances have been made in pig-to-rhesus studies from the immunological perspective. Most successful preclinical trials have been carried out with corneas of the Seoul National University (SNU) miniature pig (SNU pig, genetically unmodified) as donor tissues; however, there has been no biophysico-functional evaluation of the SNU pig cornea as a substitute for human cornea. The purpose of this study was to investigate the biophysical and functional compatibility of SNU pig cornea for use in human clinical trials...
May 2016: Xenotransplantation
Hideaki Yokogawa, Maolong Tang, Yan Li, Liang Liu, Winston Chamberlain, David Huang
PURPOSE: The goals of this laboratory study were to evaluate the interface quality in laser-assisted lamellar anterior keratoplasty (LALAK) with microkeratome-cut grafts and achieve good graft-host apposition. METHODS: Simulated LALAK surgeries were performed on 6 pairs of eye-bank corneoscleral discs. Anterior lamellar grafts were precut with microkeratomes. Deep femtosecond (FS) laser cuts were performed on host corneas followed by excimer laser smoothing. Different parameters of FS laser cuts and excimer laser smoothing were tested...
May 2016: Cornea
Nadine Gerber-Hollbach, Jack Parker, Lamis Baydoun, Vasilios S Liarakos, Lisanne Ham, Isabel Dapena, Gerrit Rj Melles
BACKGROUND/AIMS: To evaluate the clinical outcome of a full-diameter, untrephined, semicircular Descemet graft in a consecutive series of Descemet membrane endothelial keratoplasty (hemi-DMEK), potentially allowing the harvesting of two grafts from a single donor corneoscleral rim. METHODS: Interventional case series of 10 eyes of 10 patients with Fuchs endothelial dystrophy. Best corrected visual acuity (BCVA), endothelial cell density (ECD) and central corneal thickness (CCT) were evaluated up to 6 months postoperatively, and intraoperative and postoperative complications were recorded...
February 2, 2016: British Journal of Ophthalmology
Namrata Sharma, Vijay K Sharma, Tarun Arora, Kumari Reena Singh, Tushar Agarwal, Rasik B Vajpayee
PURPOSE: To describe a novel technique for removal of Descemet membrane (DM) tags or remnants in hazy corneas during Descemet stripping automated endothelial keratoplasty. METHODS: The DM tags or remnants may be difficult to visualize in the presence of hazy corneas. To identify and remove the residual DM remnants and tags, a crescent knife is introduced into the anterior chamber through the 3.2 mm temporal corneoscleral tunnel and is placed intracamerally, parallel to the iris plane underneath scored DM (which had been previously stained with the trypan blue solution)...
January 2016: Cornea
P Maier, S Heinzelmann, D Böhringer, T Reinhard
PURPOSE The number of antigen-presenting cells decreases during organ culture of corneoscleral discs. This might result in a decrease of immune reactions with increasing duration of organ culture. To investigate this hypothesis, we performed a retrospective analysis of all penetrating keratoplasties that were consecutively performed over the last 5 years.PATIENTS AND METHODS All cases of penetrating keratoplasties (n=1006) were divided into two groups, with the division made at the median of the storage time (21 days)...
January 2016: Eye
Chameen Samarawickrama, Rachel Goh, Rasik B Vajpayee
PURPOSE: To describe a new technique for harvesting freehand and horseshoe tectonic corneal grafts. METHODS: A retrospective, noncomparative interventional case series of 3 eyes from 2 patients was included. The area to be excised from the host was marked with a marking pen. Using the principle of the light table from architecture, the donor corneoscleral rim was placed over the marked area with an ophthalmic viscoelastic device in the interface to provide a coupling medium...
November 2015: Cornea
T Röck, J Hofmann, S Thaler, M Bramkamp, K U Bartz-Schmidt, E Yoeruek, D Röck
PURPOSE: The purpose was to assess the influence of donor and storage factors on the suitability of organ-cultured corneas for transplantation. METHODS: Data from 1340 donor corneas stored between 2009 and 2015 were analyzed retrospectively. Logistic regression analysis was used to assess the influence of different factors on the suitability of grafts for transplantation. RESULTS: Forty-one percent (553/1340) of corneas were discarded. The leading causes were medical contraindication (20...
January 2016: Graefe's Archive for Clinical and Experimental Ophthalmology
Efdal Yoeruek, Karl-Ulrich Bartz-Schmidt, Johanna Hofmann
PURPOSE: To evaluate the impact of the radius of the injector system on the viability of the endothelial cells in Descemet membrane endothelial keratoplasty (DMEK). METHODS: Descemet membranes (DMs) of 30 corneoscleral rims were used in a paired design and divided into three groups (A, B, C). The right corneas were used as control (without shooting through the injector after DM preparation) and the left cornea for simulation of the implantation step (shooting through a glass injector of different radii)...
February 2016: Acta Ophthalmologica
Sepehr Feizi, Mohammad Ali Javadi
PURPOSE: To compare 2 sites of air injection to prepare donor lenticules for Descemet membrane (DM) endothelial keratoplasty. METHODS: Fifty-one human corneoscleral buttons from donors aged 4 to 57 years were used. Corneoscleral buttons were placed endothelial side up and a 27-G needle was inserted just outside Schwalbe line and advanced immediately beneath the endothelium. Air was injected centrally inside the 8-mm zone (group 1) or peripherally approximately 1 mm in front of the limbus (group 2)...
January 2016: European Journal of Ophthalmology
Geetha Iyer, Bhaskar Srinivasan, Ekta Rishi, Pukhraj Rishi, Shweta Agarwal, Nirmala Subramanian
PURPOSE: To report the outcome of large tectonic lamellar grafts in cases of severe chemical injury associated with corneal melt/perforation and scleral ischemia, with or without retinal detachment. METHODS: This was a retrospective interventional analysis of 7 eyes of 6 patients within 3 months of injury with simultaneous/subsequent surgical procedures to salvage globe integrity. RESULTS: Of 7 included eyes, both eyes of one patient underwent phthisical changes...
January 2016: European Journal of Ophthalmology
Dimitrios Tsoukanas, Paraskevi Xanthopoulou, Alexandros C Charonis, Panagiotis Theodossiadis, Gerasimos Kopsinis, Theodoros Filippopoulos
PURPOSE: To determine the safety and efficacy of fresh, human sclera allografts as a patch graft material in glaucoma drainage device (GDD) surgery. DESIGN: Retrospective, noncomparative, interventional, consecutive case series. SUBJECTS: All GDD cases operated between 2008 and 2013 in which fresh human corneoscleral rims were used immediately after the central corneal button was used for penetrating or endothelial keratoplasty. METHODS: Surgery was performed by 2 surgeons at 2 facilities...
July 2016: Journal of Glaucoma
Siamak Delfazayebaher, Sepehr Feizi, Mohammad Ali Javadi, Alireza Baradaran-Rafii, Mohammad Mehdi Sadoughi, Amir Faramarzi
PURPOSE: To describe a sign that can confirm the correct position of donor lenticules during Descemet stripping automated endothelial keratoplasty (DSAEK). METHODS: In this clinical and laboratory study, DSAEK was performed in 3 eyes with a diagnosis of pseudophakic bullous keratopathy. Additionally, 2 whole globes and 2 precut corneoscleral buttons were procured for the purpose of the laboratory part of the study. After removal of recipient Descemet membrane, the donor graft was inserted into the anterior chamber, unfolded, and attached to the posterior corneal stroma with an air bubble...
August 2015: Cornea
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