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Brassica rapa clubroot

Wenxing Pang, Pengyu Fu, Xiaonan Li, Zongxiang Zhan, Sha Yu, Zhongyun Piao
The rapid spread of clubroot disease, which is caused by Plasmodiophora brassicae , threatens Brassicaceae crop production worldwide. Breeding plants that have broad-spectrum disease resistance is one of the best ways to prevent clubroot. In the present study, eight Chinese cabbage germplasms were screened using published clubroot-resistant (CR) loci-/gene-linked markers. A CR gene Crr3 potential carrier "85-74" was detected which linked to marker BRSTS61; however, "85-74" shows different responses to local pathogens "LAB-19," "LNND-2," and "LAB-10" from "CR-73" which harbors Crr3...
2018: Frontiers in Plant Science
Jingjing Chen, Yinglan Piao, Yiming Liu, Xiaonan Li, Zhongyun Piao
Chitinases, a category of pathogenesis-related proteins, are responsible for catalyzing the hydrolysis of chitin into the N-acetyl-d-glucosamine. Therefore, chitinases are believed to function as a guardian against chitin-containing pathogens. Here, we examined the role of the Brassica rapa chitinase family genes in clubroot disease. A total of 33 chitinase genes were identified and grouped into five classes based on their conserved domain. They were distributed unevenly across eight chromosomes in B. rapa, and 31 of them contained few introns (≤2)...
May 2018: Plant Science: An International Journal of Experimental Plant Biology
Hong Li, Xiaonan Li, Yuanhu Xuan, Jing Jiang, Yangdou Wei, Zhongyun Piao
Plasmodiophora brassicae is a soil borne pathogen and the causal agent of clubroot, a devastating disease of Brassica crops. The pathogen lives inside roots, and hijacks nutrients from the host plants. It is suggested that clubroot galls created an additional nutrient sink in infected roots. However, the molecular mechanism underlying P. brassicae infection and sugar transport is unclear. Here, we analyzed sugar contents in leaves and roots before and after P. brassicae infection using a pair of Chinese cabbage near-isogenic lines (NILs), carrying either a clubroot resistant (CR) or susceptible (CS) allele at the CRb locus...
2018: Frontiers in Plant Science
Yinglan Piao, Kaining Jin, Ying He, Jiaxiu Liu, Shuang Liu, Xiaonan Li, Zhongyun Piao
Mitogen-activated protein kinase (MAPK or MPK) cascades play key roles in responses to various biotic stresses, as well as in plant growth and development. However, the responses of MPK and MPK kinase (MKK) in Chinese cabbage (Brassica rapa ssp. pekinensis) to Plasmodiophora brassicae, a causal agent of clubroot disease in Brassica crops, are still not clear. In the present study, a total of 11 B. rapa MKK (BraMKK) and 30 BraMPK genes were identified and unevenly distributed in 6 and 10 chromosomes, respectively...
2018: PloS One
Fengqun Yu, Xingguo Zhang, Gary Peng, Kevin C Falk, Stephen E Strelkov, Bruce D Gossen
Clubroot, caused by Plasmodiophora brassicae, is an important disease of Brassica crops worldwide. F1 progeny from the Brassica rapa lines T19 (resistant) × ACDC (susceptible) were backcrossed with ACDC, then self-pollinated to produce BC1S1 lines, From genotyping-by-sequencing (GBS) of the parental lines and BC1 plants, about 1.32 M sequences from T19 were aligned into the reference genome of B. rapa with 0.4-fold coverage, and 1.77 M sequences with 0.5-fold coverage in ACDC. The number of aligned short reads per plant in the BC1 ranged from 0...
July 3, 2017: Scientific Reports
Rawnak Laila, Arif Hasan Khan Robin, Kiwoung Yang, Gyung Ja Choi, Jong-In Park, Ill-Sup Nou
Clubroot is a soil-borne disease caused by the protist Plasmodiophora brassicae (P. brassicae). It is one of the most economically important diseases of Brassica rapa and other cruciferous crops as it can cause remarkable yield reductions. Understanding P. brassicae genetics, and developing efficient molecular markers, is essential for effective detection of harmful races of this pathogen. Samples from 11 Korean field populations of P. brassicae (geographic isolates), collected from nine different locations in South Korea, were used in this study...
January 4, 2017: International Journal of Molecular Sciences
Katsunori Hatakeyama, Tomohisa Niwa, Takeyuki Kato, Takayoshi Ohara, Tomohiro Kakizaki, Satoru Matsumoto
To facilitate prevention of clubroot disease, a major threat to the successful cultivation of Chinese cabbage (Brassica rapa L.), we bred clubroot-resistant (CR) cultivars by introducing resistance genes from CR turnips via conventional breeding. Among 11 CR loci found in B. rapa, we identified CRb in Chinese cabbage cultivar 'CR Shinki' as a single dominant gene for resistance against Plasmodiophora brassicae pathotype group 3, against which the stacking of Crr1 and Crr2 loci was not effective. However, the precise location and pathotype specificity of CRb have been controversial, because CRa and Rcr1 also map near this locus...
April 2017: Molecular Genetics and Genomics: MGG
Tao Song, Mingguang Chu, Rachid Lahlali, Fengqun Yu, Gary Peng
Clubroot, caused by the plasmodiophorid pathogen Plasmodiophora brassicae, is one of the most serious diseases on Brassica crops worldwide and a major threat to canola production in western Canada. Host resistance is the key strategy for clubroot management on canola. Several clubroot resistance (CR) genes have been identified, but the mechanisms associated with these CR genes are poorly understood. In the current study, a label-free shotgun proteomic approach was used to profile and compare the proteomes of Brassica rapa carrying and not carrying the CR gene Rcr1 in response to P...
2016: Frontiers in Plant Science
Fengqun Yu, Xingguo Zhang, Zhen Huang, Mingguang Chu, Tao Song, Kevin C Falk, Abhinandan Deora, Qilin Chen, Yan Zhang, Linda McGregor, Bruce D Gossen, Mary Ruth McDonald, Gary Peng
Clubroot, caused by Plasmodiophora brassicae, is an important disease on Brassica species worldwide. A clubroot resistance gene, Rcr1, with efficacy against pathotype 3 of P. brassicae, was previously mapped to chromosome A03 of B. rapa in pak choy cultivar "Flower Nabana". In the current study, resistance to pathotypes 2, 5 and 6 was shown to be associated with Rcr1 region on chromosome A03. Bulked segregant RNA sequencing was performed and short read sequences were assembled into 10 chromosomes of the B. rapa reference genome v1...
2016: PloS One
Kazutaka Kawamura, Takahiro Kawanabe, Motoki Shimizu, Keiichi Okazaki, Makoto Kaji, Elizabeth S Dennis, Kenji Osabe, Ryo Fujimoto
Chinese cabbage (Brassica rapa L. var. pekinensis) is an important vegetable in Asia, and most Japanese commercial cultivars of Chinese cabbage use an F1 hybrid seed production system. Self-incompatibility is successfully used for the production of F1 hybrid seeds in B. rapa vegetables to avoid contamination by non-hybrid seeds, and the strength of self-incompatibility is important for harvesting a highly pure F1 seeds. Prediction of agronomically important traits such as disease resistance based on DNA markers is useful...
March 2016: Data in Brief
Jonghoon Lee, Nur Kholilatul Izzah, Beom-Soon Choi, Ho Jun Joh, Sang-Choon Lee, Sampath Perumal, Joodeok Seo, Kyounggu Ahn, Eun Ju Jo, Gyung Ja Choi, Ill-Sup Nou, Yeisoo Yu, Tae-Jin Yang
Clubroot is a devastating disease caused by Plasmodiophora brassicae and results in severe losses of yield and quality in Brassica crops. Many clubroot resistance genes and markers are available in Brassica rapa but less is known in Brassica oleracea. Here, we applied the genotyping-by-sequencing (GBS) technique to construct a high-resolution genetic map and identify clubroot resistance (CR) genes. A total of 43,821 SNPs were identified using GBS data for two parental lines, one resistant and one susceptible lines to clubroot, and 18,187 of them showed >5× coverage in the GBS data...
February 2016: DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes
Mingguang Chu, Tao Song, Kevin C Falk, Xingguo Zhang, Xunjia Liu, Adrian Chang, Rachid Lahlali, Linda McGregor, Bruce D Gossen, Gary Peng, Fengqun Yu
BACKGROUND: The protist Plasmodiophora brassicae is a biotrophic soil-borne pathogen that causes clubroot on Brassica crops worldwide. Clubroot disease is a serious threat to the 8 M ha of canola (Brassica napus) grown annually in western Canada. While host resistance is the key to clubroot management, sources of resistance are limited. RESULTS: To identify new sources of clubroot resistance (CR), we fine mapped a CR gene (Rcr1) from B. rapa ssp. chinensis to the region between 24...
2014: BMC Genomics
Rachid Lahlali, Linda McGregor, Tao Song, Bruce D Gossen, Kazuhiko Narisawa, Gary Peng
An endophytic fungus, Heteroconium chaetospira isolate BC2HB1 (Hc), suppressed clubroot (Plasmodiophora brassicae -Pb) on canola in growth-cabinet trials. Confocal microscopy demonstrated that Hc penetrated canola roots and colonized cortical tissues. Based on qPCR analysis, the amount of Hc DNA found in canola roots at 14 days after treatment was negatively correlated (r = 0.92, P<0.001) with the severity of clubroot at 5 weeks after treatment at a low (2×10(5) spores pot(-1)) but not high (2×10(5) spores pot(-1)) dose of pathogen inoculum...
2014: PloS One
Jingjing Chen, Jing Jing, Zhongxiang Zhan, Teng Zhang, Chunyu Zhang, Zhongyun Piao
Plasmodiophora brassicae, the causal agent of clubroot disease of the Brassica crops, is widespread in the world. Quantitative trait loci (QTLs) for partial resistance to 4 different isolates of P. brassicae (Pb2, Pb4, Pb7, and Pb10) were investigated using a BC1F1 population from a cross between two subspecies of Brassica rapa, i.e. Chinese cabbage inbred line C59-1 as a susceptible recurrent parent and turnip inbred line ECD04 as a resistant donor parent. The BC1F2 families were assessed for resistance under controlled conditions...
2013: PloS One
Takeyuki Kato, Katsunori Hatakeyama, Nobuko Fukino, Satoru Matsumoto
In Chinese cabbage (Brassica rapa), the clubroot resistance (CR) gene CRb is effective against Plasmodiophora brassicae isolate No. 14, which is classified as pathotype group 3. Although markers linked to CRb have been reported, an accurate position in the genome and the gene structure are unknown. To determine the genomic location and estimate the structure of CRb, we developed 28 markers (average distance, 20.4 kb) around CRb and constructed a high-density partial map. The precise position of CRb was determined by using a population of 2,032 F2 plants generated by selfing B...
March 2013: Breeding Science
Katsunori Hatakeyama, Keita Suwabe, Rubens Norio Tomita, Takeyuki Kato, Tsukasa Nunome, Hiroyuki Fukuoka, Satoru Matsumoto
Clubroot disease, caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin, is one of the most economically important diseases of Brassica crops in the world. Although many clubroot resistance (CR) loci have been identified through genetic analysis and QTL mapping, the molecular mechanisms of defense responses against P. brassicae remain unknown. Fine mapping of the Crr1 locus, which was originally identified as a single locus, revealed that it comprises two gene loci, Crr1a and Crr1b. Here we report the map-based cloning and characterization of Crr1a, which confers resistance to clubroot in Brassica rapa...
2013: PloS One
Takeyuki Kato, Katsunori Hatakeyama, Nobuko Fukino, Satoru Matsumoto
In Chinese cabbage (Brassica rapa), the clubroot resistance (CR) genes Crr1 and Crr2 are effective against the mild Plasmodiophora brassicae isolate Ano-01 and the more virulent isolate Wakayama-01, but not against isolate No. 14, classified into pathotype group 3. 'Akiriso', a clubroot-resistant F(1) cultivar, showed resistance to isolate No. 14. To increase the durability of resistance, we attempted to identify the CR locus in 'Akiriso'. CR in 'Akiriso' segregated as a single dominant gene and was linked to several molecular markers that were also linked to CRb, a CR locus from cultivar 'CR Shinki'...
September 2012: Breeding Science
Keita Suwabe, Go Suzuki, Tsukasa Nunome, Katsunori Hatakeyama, Yasuhiko Mukai, Hiroyuki Fukuoka, Satoru Matsumoto
Genome evolution is a continuous process and genomic rearrangement occurs both within and between species. With the sequencing of the Arabidopsis thaliana genome, comparative genetics and genomics offer new insights into plant biology. The genus Brassica offers excellent opportunities with which to compare genomic synteny so as to reveal genome evolution. During a previous genetic analysis of clubroot resistance in Brassica rapa, we identified a genetic region that is highly collinear with Arabidopsis chromosome 4...
June 2012: Breeding Science
Hiroki Ueno, Etsuo Matsumoto, Daisuke Aruga, Satoshi Kitagawa, Hideo Matsumura, Nobuaki Hayashida
Clubroot disease is one of the major diseases affecting Brassicaceae crops, and a number of these crops grown commercially, such as Chinese cabbage (Brassica rapa L. ssp. pekinensis), are known to be highly susceptible to clubroot disease. To provide protection from this disease, plant breeders have introduced genes for resistance to clubroot from the European turnip into susceptible lines. The CRa gene confers specific resistance to the clubroot pathogen Plasmodiophora brassicae isolate M85. Fine mapping of the CRa locus using synteny to the Arabidopsis thaliana genome and partial genome sequences of B...
December 2012: Plant Molecular Biology
Geoffrey Wagner, Sophie Charton, Christine Lariagon, Anne Laperche, Raphaël Lugan, Julie Hopkins, Pierre Frendo, Alain Bouchereau, Régine Delourme, Antoine Gravot, Maria J Manzanares-Dauleux
Clubroot disease affects all Brassicaceae spp. and is caused by the obligate biotroph pathogen Plasmodiophora brassicae. The development of galls on the root system is associated with the establishment of a new carbon metabolic sink. Here, we aimed to deepen our knowledge of the involvement of primary metabolism in the Brassica napus response to clubroot infection. We studied the dynamics and the diversity of the metabolic responses to the infection. Root system metabotyping was carried out for 18 rapeseed genotypes displaying different degrees of symptom severity, under inoculated and noninoculated conditions at 42 days postinoculation (dpi)...
November 2012: Molecular Plant-microbe Interactions: MPMI
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