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Brassica rapa

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https://www.readbyqxmd.com/read/29134463/stir-frying-of-chinese-cabbage-and-pakchoi-retains-health-promoting-glucosinolates
#1
Probo Y Nugrahedi, Teresa Oliviero, Jenneke K Heising, Matthijs Dekker, Ruud Verkerk
Stir-frying is a cooking method, originating from Asia, in which food is fried in small amount of very hot oil. Nowadays in many other parts of the world stir-frying is a very popular method to prepare vegetables, because it is fast and fried vegetables are tasty. However, the retention of phytochemicals like the health-beneficial glucosinolates in Brassica vegetables is less explored for stir-frying in comparison to other cooking methods. This study investigates the retention of glucosinolates in Chinese cabbage (Brassica rapa ssp...
November 13, 2017: Plant Foods for Human Nutrition
https://www.readbyqxmd.com/read/29132315/comprehensive-genomic-analysis-of-the-cngc-gene-family-in-brassica-oleracea-novel-insights-into-synteny-structures-and-transcript-profiles
#2
Kaleem U Kakar, Zarqa Nawaz, Khadija Kakar, Essa Ali, Abdulwareth A Almoneafy, Raqeeb Ullah, Xue-Liang Ren, Qing-Yao Shu
BACKGROUND: The cyclic nucleotide-gated ion channel (CNGC) family affects the uptake of cations, growth, pathogen defence, and thermotolerance in plants. However, the systematic identification, origin and function of this gene family has not been performed in Brassica oleracea, an important vegetable crop and genomic model organism. RESULTS: In present study, we identified 26 CNGC genes in B. oleracea genome, which are non-randomly localized on eight chromosomes, and classified into four major (I-IV) and two sub-groups (i...
November 13, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29128880/the-immunophilin-repertoire-of-plasmodiophora-brassicae-and-functional-analysis-of-pbcyp3-cyclophilin
#3
Khushwant Singh, Georgios Tzelepis, Miloslav Zouhar, Pavel Ryšánek, Christina Dixelius
Plasmodiophora brassicae is a soil-borne pathogen that belongs to Rhizaria, an almost unexplored eukaryotic organism group. This pathogen requires a living host for growth and multiplication, which makes molecular analysis further complicated. To broaden our understanding of a plasmodiophorid such as P. brassicae, we here chose to study immunophilins, a group of proteins known to have various cellular functions, including involvement in plant defense and pathogen virulence. Searches in the P. brassicae genome resulted in 20 putative immunophilins comprising of 11 cyclophilins (CYPs), 7 FK506-binding proteins (FKBPs) and 2 parvulin-like proteins...
November 11, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29123974/expression-profiles-of-brmyb-transcription-factors-related-to-glucosinolate-biosynthesis-and-stress-response-in-eight-subspecies-of-brassica-rapa
#4
Mi-Suk Seo, Mina Jin, Seong-Han Sohn, Jung Sun Kim
Brassica rapa is a polyploid species with phenotypically diverse cultivated subspecies. Glucosinolates (GSLs) are secondary metabolites that contribute to anticarcinogenic activity and plant defense in Brassicaceae. Previously, complete coding sequences of 13 BrMYB transcription factors (TFs) related to GSL biosynthesis were identified in the B. rapa genome. In the present study, we investigated GSL content and expression levels of these BrMYB TFs in 38 accessions belonging to eight subspecies of B. rapa. Twelve identified GSLs were detected and were classified into three chemical groups based on patterns of GSL content and expression profiles of the BrMYB TFs...
November 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/29119365/pectin-methylesterase-inhibitor-pmei-family-can-be-related-to-male-sterility-in-chinese-cabbage-brassica-rapa-ssp-pekinensis
#5
Chong Tan, Zhiyong Liu, Shengnan Huang, Chengyu Li, Jie Ren, Xiaoyan Tang, Wenjie Liu, Shenling Peng, Hui Feng
Pectin-related genes play significant roles in pollen development and pollen tube growth, and their allelic variations are one of the major reasons for the abnormal development of male gametophyte. Currently, little is known about the role of the PMEI family in male sterility of plants. In this study, 97 putative PMEI genes were identified in Brassica rapa genome. By a phylogenetic analysis, the PMEI family was divided into 10 clades with highly conserved structural characteristics. The publically available RNA-seq data on different tissues of B...
November 8, 2017: Molecular Genetics and Genomics: MGG
https://www.readbyqxmd.com/read/29098952/a-combination-of-absorption-and-enzymatic-biodegradation-phenol-elimination-from-aqueous-and-organic-phase
#6
Tandjaoui Nassima, Abouseoud Mahmoud, Couvert Annabelle, Amrane Abdeltif, Tassist Amina
Peroxidase from Brassica rapa (BRP) was immobilized as cross-linked enzyme aggregates (CLEAs) and used to treat air containing phenol as a model molecule of volatile organic compounds (VOCs). Prior to enzymatic treatment, phenol was absorbed into aqueous or organic phase (silicone oil, Rhodorsil® 47V20) to reach concentrations ranging from 20 to 160mg/L. The process of enzymatic degradation was carried out by introducing a desired weighing of BRP-CLEAs into preparations and reaction was started by injecting H2O2 solution to medium...
November 3, 2017: Environmental Technology
https://www.readbyqxmd.com/read/29096605/genome-wide-identification-and-expression-analysis-of-calmodulin-like-cml-genes-in-chinese-cabbage-brassica-rapa-l-ssp-pekinensis
#7
Shanshan Nie, Minjuan Zhang, Lugang Zhang
BACKGROUND: Calmodulin-like (CML) proteins are a primary family of plant-specific Ca(2+) sensors that specifically bind to Ca(2+) and deliver a Ca(2+) signal. CML proteins have been identified and characterized in many plant species, such as the model plant Arabidopsis and rice. Based on considerable evidence, the roles of CML proteins are crucial in plant growth and development and in the response to various external stimuli. Nevertheless, the characterization and expression profiling of CML genes in Chinese cabbage (Brassica rapa L...
November 2, 2017: BMC Genomics
https://www.readbyqxmd.com/read/29089948/flowering-time-gene-variation-in-brassica-species-shows-evolutionary-principles
#8
Sarah V Schiessl, Bruno Huettel, Diana Kuehn, Richard Reinhardt, Rod J Snowdon
Flowering time genes have a strong influence on successful reproduction and life cycle adaptation. However, their regulation is highly complex and only well understood in diploid model systems. For crops with a polyploid background from the genus Brassica, data on flowering time gene variation are scarce, although indispensable for modern breeding techniques like marker-assisted breeding. We have deep-sequenced all paralogs of 35 Arabidopsis thaliana flowering regulators using Sequence Capture followed by Illumina sequencing in two selected accessions of the vegetable species Brassica rapa and Brassica oleracea, respectively...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29069614/increased-biomass-and-quality-and-reduced-heavy-metal-accumulation-of-edible-tissues-of-vegetables-in-the-presence-of-cd-tolerant-and-immobilizing-bacillus-megaterium-h3
#9
Qi Wang, Wen-Ji Zhang, Lin-Yan He, Xia-Fang Sheng
A Cd-resistant and immobilizing Bacillus megaterium H3 was characterized for its impact on the biomass and quality and heavy metal uptake of edible tissues of two vegetables (Brassica campestris L. var. Aijiaohuang and Brassica rapa L. var. Shanghaiqing) grown in heavy metal-polluted soil. The impact of strain H3 on the soil quality was also evaluated. The increase in the edible tissue biomass and the contents of soluble proteins and vitamin C of the vegetables inoculated with strain H3 ranged from 18% to 33%, 17% to 31%, and 15% to 19%, respectively, compared with the controls...
October 22, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29058049/bayesian-estimation-and-use-of-high-throughput-remote-sensing-indices-for-quantitative-genetic-analyses-of-leaf-growth
#10
Robert L Baker, Wen Fung Leong, Nan An, Marcus T Brock, Matthew J Rubin, Stephen Welch, Cynthia Weinig
We develop Bayesian function-valued trait models that mathematically isolate genetic mechanisms underlying leaf growth trajectories by factoring out genotype-specific differences in photosynthesis. Remote sensing data can be used instead of leaf-level physiological measurements. Characterizing the genetic basis of traits that vary during ontogeny and affect plant performance is a major goal in evolutionary biology and agronomy. Describing genetic programs that specifically regulate morphological traits can be complicated by genotypic differences in physiological traits...
October 20, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29038660/genetic-regulation-of-ga-metabolism-during-vernalization-floral-bud-initiation-and-development-in-pak-choi-brassica-rapa-ssp-chinensis-makino
#11
Mengya Shang, Xueting Wang, Jing Zhang, Xianhui Qi, Amin Ping, Leiping Hou, Guoming Xing, Gaizhen Li, Meilan Li
Pak choi (Brassica rapa ssp. chinensis Makino) is a representative seed vernalization vegetable and premature bolting in spring can cause significant economic loss. Thus, it is critical to elucidate the mechanism of molecular regulation of vernalization and floral bud initiation to prevent premature bolting. Gibberellin (GA) is the key plant hormone involved in regulating plant development. To gain a better understanding of GA metabolism in pak choi, the content of GA in pak choi was measured at different stages of plant development using enzyme-linked immunosorbent assay...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29025408/synteny-analysis-of-genes-and-distribution-of-loci-controlling-oil-content-and-fatty-acid-profile-based-on-qtl-alignment-map-in-brassica-napus
#12
Nadia Raboanatahiry, Hongbo Chao, Liangxing Guo, Jianping Gan, Jun Xiang, Mingli Yan, Libin Zhang, Longjiang Yu, Maoteng Li
BACKGROUND: Deciphering the genetic architecture of a species is a good way to understand its evolutionary history, but also to tailor its profile for breeding elite cultivars with desirable traits. Aligning QTLs from diverse population in one map and utilizing it for comparison, but also as a basis for multiple analyses assure a stronger evidence to understand the genetic system related to a given phenotype. RESULTS: In this study, 439 genes involved in fatty acid (FA) and triacylglycerol (TAG) biosyntheses were identified in Brassica napus...
October 12, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28994289/structural-characterization-and-immunostimulatory-activity-of-polysaccharides-from-brassica-rapa-l-%C3%A2
#13
Zhuo-Er Chen, Reziyamu Wufuer, Jin-Hu Ji, Jin-Fang Li, Yu-Feng Cheng, Cai-Xia Dong, Hailiqian Taoerdahong
Two neutral polysaccharides (BRNP-1, 6.9 kDa; BRNP-2, 4.8 kDa) were purified from the common edible plant Brassica rapa L. via the combined techniques of ion-exchange chromatography and high-performance gel permeation chromatography. Monosaccharide composition analysis showed that BRNP-1 and BRNP-2 were composed of glucosyl residues. Methylation and 1D- and 2D-NMR analyses revealed that both BRNP-1 and BRNP-2 contained a backbone chain that was composed of α-D-(1 → 4)-linked Glcp residues and side chains that were composed of terminally linked Glcp residues attached at the O-6 position of backbone-glycosyl residues...
October 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28976303/into-the-fourth-dimension
#14
David L Des Marais
The influence of time on the drought response of Brassica rapa, an agriculturally important species of plant, has been clarified.
October 4, 2017: ELife
https://www.readbyqxmd.com/read/28966627/identification-of-optimal-reference-genes-for-expression-analysis-in-radish-raphanus-sativus-l-and-its-relatives-based-on-expression-stability
#15
Mengmeng Duan, Jinglei Wang, Xiaohui Zhang, Haohui Yang, Haiping Wang, Yang Qiu, Jiangping Song, Yangdong Guo, Xixiang Li
Radish (Raphanus sativus) is an important cruciferous root crop with a close relationship to Chinese cabbage (Brassica rapa). RT-qPCR is used extensively to evaluate the expression levels of target genes, and accurate measurement of target gene expression with this method is determined by the valid reference genes used for data nomalization in different experimental conditions. Screening for appropriate reference genes with stable expression based on RT-qPCR data is important for gene expression and functional analysis research in radish and its relatives...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28964362/metabolic-profiling-of-glucosinolates-and-their-hydrolysis-products-in-a-germplasm-collection-of-brassica-rapa-turnips
#16
Rebecca Klopsch, Katja Witzel, Andreas Börner, Monika Schreiner, Franziska S Hanschen
About 10% of the world's vegetable production is generated from Brassicaceae, wherein Brassica rapa is a dominating species. There is growing evidence that glucosinolates (GLSs), main plant secondary metabolites in Brassicales, play an important role in promoting human health. Natural genetic diversity of B. rapa can be explored for vegetable improvement. We analyzed leaves and tubers of 16 B. rapa turnips for their GLS composition by UHPLC-DAD and the corresponding hydrolysis products by GC-MS. Thirteen GLSs were identified, 8 aliphatic, 4 indolic and one aromatic...
October 2017: Food Research International
https://www.readbyqxmd.com/read/28961511/identification-and-functional-prediction-of-stress-responsive-ap2-erf-transcription-factors-in-brassica-napus-by-genome-wide-analysis
#17
Hajar Owji, Ali Hajiebrahimi, Hassan Seradj, Shiva Hemmati
Using homology and domain authentication, 321 putative AP2/ERF transcription factors were identified in Brassica napus, called BnAP2/ERF TFs. BnAP2/ERF TFs were classified into five major subfamilies, including DREB, ERF, AP2, RAV, and BnSoloist. This classification is based on phylogenetic analysis, motif identification, gene structure analysis, and physiochemical characterization. These TFs were annotated based on phylogenetic relationship with Brassica rapa. BnAP2/ERF TFs were located on 19 chromosomes of B...
September 14, 2017: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/28959639/assessment-of-the-potential-health-risks-associated-with-the-aluminium-arsenic-cadmium-and-lead-content-in-selected-fruits-and-vegetables-grown-in-jamaica
#18
Johann M R Antoine, Leslie A Hoo Fung, Charles N Grant
Thirteen Jamaican-grown food crops - ackee (Blighia sapida), banana (Musa acuminate), cabbage (Brassica oleracea), carrot (Daucus carota), cassava (Manihot esculenta), coco (Xanthosoma sagittifolium), dasheen (Colocasia esculenta), Irish potato (Solanum tuberosum), pumpkin (Cucurbita pepo), sweet pepper (Capsicum annuum), sweet potato (Ipomoea batatas), tomato (Solanum lycopersicum) and turnip (Brassica rapa) - were analysed for aluminium, arsenic, cadmium and lead by atomic absorption spectrophotometry and instrumental neutron activation analysis...
2017: Toxicology Reports
https://www.readbyqxmd.com/read/28959349/computational-identification-of-micrornas-and-their-transcript-target-s-in-field-mustard-brassica-rapa-l
#19
Behzad Hajieghrari, Naser Farrokhi, Bahram Goliaei, Kaveh Kavousi
BACKGROUND: Micro RNAs (miRNAs) are a pivotal part of non-protein-coding endogenous small RNA molecules that regulate the genes involved in plant growth and development, and respond to biotic and abiotic environmental stresses posttranscriptionally. OBJECTIVE: In the present study, we report the results of a systemic search for identification of new miRNAs in B. rapa using homology-based ESTs (Expressed Sequence Tags) analysis and considering a series of fi ltration criteria...
March 2017: Iranian journal of biotechnology
https://www.readbyqxmd.com/read/28955373/genome-wide-identification-and-characterization-of-brrtcp-transcription-factors-in-brassica-rapa-ssp-rapa
#20
Jiancan Du, Simin Hu, Qin Yu, Chongde Wang, Yunqiang Yang, Hang Sun, Yongping Yang, Xudong Sun
The teosinte branched1/cycloidea/proliferating cell factor (TCP) gene family is a plant-specific transcription factor that participates in the control of plant development by regulating cell proliferation. However, no report is currently available about this gene family in turnips (Brassica rapa ssp. rapa). In this study, a genome-wide analysis of TCP genes was performed in turnips. Thirty-nine TCP genes in turnip genome were identified and distributed on 10 chromosomes. Phylogenetic analysis clearly showed that the family was classified as two clades: class I and class II...
2017: Frontiers in Plant Science
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