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Brassica rapa

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https://www.readbyqxmd.com/read/28332757/selective-modes-determine-evolutionary-rates-gene-compactness-and-expression-patterns-in-brassica
#1
Yue Guo, Jing Liu, Jiefu Zhang, Shengyi Liu, Jianchang Du
It has been well documented that most nuclear protein-coding genes in organisms can be classified into two categories, i.e., positively selected genes (PSGs) and negatively selected genes (NSGs). The characteristics and evolutionary fates of different types of genes, however, have been poorly understood. In this study, the rates of nonsynonymous substitution (Ka) and the synonymous substitution (Ks) were investigated by comparing the orthologs between the two sequenced Brassica species, Brassica rapa, and Brassica oleracea, and the evolutionary rates, gene structures, expression patterns, and codon bias were compared between PSGs and NSGs...
March 23, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28330247/production-of-glucosinolates-phenolic-compounds-and-associated-gene-expression-profiles-of-hairy-root-cultures-in-turnip-brassica-rapa-ssp-rapa
#2
Ill-Min Chung, Kaliyaperumal Rekha, Govindasamy Rajakumar, Muthu Thiruvengadam
Turnip (Brassica rapa ssp. rapa) is an important vegetable crop producing glucosinolates (GSLs) and phenolic compounds. The GSLs, phenolic compound contents and transcript levels in hairy root cultures, as well as their antioxidant, antimicrobial and anticancer activity were studied in turnip. Transgenic hairy root lines were confirmed by polymerase chain reaction (PCR) and reverse transcription-PCR. GSLs levels (glucoallysin, glucobrassicanapin, gluconasturtiin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and 4-hydroxyglucobrassicin) and their gene expression levels (BrMYB28, BrMYB29, BrMYB34, BrMYB51, BrMYB122, CYP79 and CYP83) significantly increased in hairy roots compared with that in non-transformed roots...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330158/a-simple-and-efficient-agrobacterium-tumefaciens-mediated-plant-transformation-of-brassica-rapa-ssp-pekinensis
#3
Venkidasamy Baskar, Baniekal H Gangadhar, Se Won Park, Shivraj Hariram Nile
The present study aims to investigate the numerous factors influencing Agrobacterium tumefaciens-mediated genetic transformation of Chinese cabbage (Brassica rapa ssp. pekinensis). Factors affecting transformation efficiency, such as age of explants, Agrobacterium concentration, and effect of acetosyringone, pre-cultivation, infection and co-cultivation time of Agrobacterium were examined. The pre-cultured hypocotyls from young seedlings prior to exposure to Agrobacterium showed higher shoot regeneration. The plant transformation with the modest A...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28324819/impact-of-different-feedstocks-derived-biochar-amendment-with-cadmium-low-uptake-affinity-cultivar-of-pak-choi-brassica-rapa-ssb-chinensis-l-on-phytoavoidation-of-cd-to-reduce-potential-dietary-toxicity
#4
Kiran Yasmin Khan, Barkat Ali, Xiaoqiang Cui, Ying Feng, Xiaoe Yang, Peter Joseph Stoffella
Biochar has become eco-friendly amendment used for phytoavoidation with low cadmium (Cd) accumulating cultivars of crops to ensure food safety in Cd contaminated soils. In this study, biochar with different waste feedstock material were evaluated for their effectiveness on essential trace metals mobility, Cd bioavailability and its accumulation in two contrasting Cd accumulating cultivars of pak choi (Brassica rapa ssp. chinensis L.) grown in Cd contaminated Mollisol soil. A greenhouse experiment was conducted with plants grown in Cd contaminated soil that had been amended with biochar derived from barley straw, tomato green waste, chicken manure, duck manure and swine manure at application rate of 0%, 2...
March 18, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28316286/brassica-aphid-hemiptera-aphididae-populations-are-conditioned-by-climatic-variables-and-parasitism-level-a-study-case-of-tri%C3%A3-ngulo-mineiro-brazil
#5
M V Sampaio, A P Korndörfer, J Pujade-Villar, J E A Hubaide, S E Ferreira, S O Arantes, D M Bortoletto, C M Guimarães, J A Sánchez-Espigares, B Caballero-López
Cosmopolitan pests such as Brevicoryne brassicae, Lipaphis pseudobrassicae, and Myzus persicae (Aphididae) cause significant damage to Brassicaceae crops. Assessment of the important biotic and abiotic factors that regulate these pests is an essential step in the development of effective Integrated Pest Management programs for these aphids. This study evaluated the influence of leaf position, precipitation, temperature, and parasitism on populations of L. pseudobrassicae, M. persicae, and B. brassicae in collard greens fields in the Triângulo Mineiro region (Minas Gerais state), Brazil...
March 20, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28291771/real-time-divergent-evolution-in-plants-driven-by-pollinators
#6
Daniel D L Gervasi, Florian P Schiestl
Pollinator-driven diversification is thought to be a major source of floral variation in plants. Our knowledge of this process is, however, limited to indirect assessments of evolutionary changes. Here, we employ experimental evolution with fast cycling Brassica rapa plants to demonstrate adaptive evolution driven by different pollinators. Our study shows pollinator-driven divergent selection as well as divergent evolution in plant traits. Plants pollinated by bumblebees evolved taller size and more fragrant flowers with increased ultraviolet reflection...
March 14, 2017: Nature Communications
https://www.readbyqxmd.com/read/28288105/reduction-of-antinutritional-glucosinolates-in-brassica-oilseeds-by-mutation-of-genes-encoding-transporters
#7
Hussam Hassan Nour-Eldin, Svend Roesen Madsen, Steven Engelen, Morten Egevang Jørgensen, Carl Erik Olsen, Jonathan Sonne Andersen, David Seynnaeve, Thalia Verhoye, Rudy Fulawka, Peter Denolf, Barbara Ann Halkier
The nutritional value of Brassica seed meals is reduced by the presence of glucosinolates, which are toxic compounds involved in plant defense. Mutation of the genes encoding two glucosinolate transporters (GTRs) eliminated glucosinolates from Arabidopsis thaliana seeds, but translation of loss-of-function phenotypes into Brassica crops is challenging because Brassica is polyploid. We mutated one of seven and four of 12 GTR orthologs and reduced glucosinolate levels in seeds by 60-70% in two different Brassica species (Brassica rapa and Brassica juncea)...
March 13, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28270829/cadmium-disrupts-the-balance-between-hydrogen-peroxide-and-superoxide-radical-by-regulating-endogenous-hydrogen-sulfide-in-the-root-tip-of-brassica-rapa
#8
Wenjing Lv, Lifei Yang, Cunfa Xu, Zhiqi Shi, Jinsong Shao, Ming Xian, Jian Chen
Cd (cadmium) stress always alters the homeostasis of ROS (reactive oxygen species) including H2O2 (hydrogen sulfide) and [Formula: see text] (superoxide radical), leading to the oxidative injury and growth inhibition in plants. In addition to triggering oxidative injury, ROS has been suggested as important regulators modulating root elongation. However, whether and how Cd stress induces the inhibition of root elongation by differentially regulating endogenous H2O2 and [Formula: see text], rather than by inducing oxidative injury, remains elusive...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28258381/a-glabra1-ortholog-on-lg-a9-controls-trichome-number-in-the-japanese-leafy-vegetables-mizuna-and-mibuna-brassica-rapa-l-subsp-nipposinica-l-h-bailey-evidence-from-qtl-analysis
#9
Yaichi Kawakatsu, Hokuto Nakayama, Kaori Kaminoyama, Kaori Igarashi, Masaki Yasugi, Hiroshi Kudoh, Atsushi J Nagano, Kentaro Yano, Nakao Kubo, Seisuke Kimura
Brassica rapa show a wide range of morphological variations. In particular, the leaf morphologies of the Japanese traditional leafy vegetables Mizuna and Mibuna (Brassica rapa L. subsp. nipposinica L. H. Bailey) are distinctly different, even though they are closely related cultivars that are easy to cross. In addition to the differences in the gross morphology of leaves, some cultivars of Mibuna (Kyo-nishiki) have many trichomes on its leaves, whereas Mizuna (Kyo-mizore) does not. To identify the genes responsible for the different number of trichomes, we performed a quantitative trait loci (QTL) analysis of Mizuna and Mibuna...
March 3, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28239387/comprehensive-analysis-of-the-cdpk-snrk-superfamily-genes-in-chinese-cabbage-and-its-evolutionary-implications-in-plants
#10
Peng Wu, Wenli Wang, Weike Duan, Ying Li, Xilin Hou
The CDPK-SnRK (calcium-dependent protein kinase/Snf1-related protein kinase) gene superfamily plays important roles in signaling pathways for disease resistance and various stress responses, as indicated by emerging evidence. In this study, we constructed comparative analyses of gene structure, retention, expansion, whole-genome duplication (WGD) and expression patterns of CDPK-SnRK genes in Brassica rapa and their evolution in plants. A total of 49 BrCPKs, 14 BrCRKs, 3 BrPPCKs, 5 BrPEPRKs, and 56 BrSnRKs were identified in B...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28210266/a-phylogenetic-analysis-of-chloroplast-genomes-elucidates-the-relationships-of-the-six-economically-important-brassica-species-comprising-the-triangle-of-u
#11
Peirong Li, Shujiang Zhang, Fei Li, Shifan Zhang, Hui Zhang, Xiaowu Wang, Rifei Sun, Guusje Bonnema, Theo J A Borm
The Brassica genus comprises many economically important worldwide cultivated crops. The well-established model of the Brassica genus, U's triangle, consists of three basic diploid plant species (Brassica rapa, Brassica oleracea, and Brassica nigra) and three amphidiploid species (Brassica napus, Brassica juncea, and Brassica carinata) that arose through interspecific hybridizations. Despite being extensively studied because of its commercial relevance, several aspects of the origin of the Brassica species and the relationships within and among these six species still remain open questions...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28185156/imi-resistance-associated-to-crop-weed-hybridization-in-a-natural-brassica-rapa-population-characterization-and-fate
#12
M S Ureta, F Torres Carbonell, C Pandolfo, A D Presotto, M A Cantamutto, M Poverene
Wild turnip (Brassica rapa) is a common weed and a close relative to oilseed rape (Brassica napus). The Clearfield® production system is a highly adopted tool which provides an alternative solution for weed management, but its efficiency is threatened by gene transfer from crop to weed relatives. Crop-weed hybrids with herbicide resistance were found in the progeny of a B. rapa population gathered from a weedy stand on the borders of an oilseed rape (B. napus) imidazolinone (IMI)-resistant crop. Interspecific hybrids were confirmed by morphological traits in the greenhouse and experimental field, survival after imazethapyr applications, DNA content through flow cytometry, and pollen viability...
March 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28177832/natural-variation-in-i-circadian-clock-associated-1-i-is-associated-with-flowering-time-in-i-brassica-rapa-i
#13
Hankuil Yi, Xiaonan Li, Seong Ho Lee, Ill-Sup Nou, Yong Pyo Lim, Yoonkang Hur
Flowering time is a very important agronomic trait and the development of molecular markers associated with this trait can facilitate crop breeding. <i>CIRCADIAN CLOCK ASSOCIATED 1</i> (<i>CCA1</i>), a core oscillator component of circadian rhythms that affect metabolic pathways in plants, has been implicated in flowering time control in <i>Brassica</i> species. <i>CCA1</i> gene sequences from three <i>Brassica rapa</i> inbred lines, showing either early flowering or late flowering phenotypes, were analyzed and a high level of ets were designed and tested using various inbred lines of <i>B...
December 22, 2016: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28169083/evaluation-of-biochar-amended-biosolids-co-composting-to-improve-the-nutrient-transformation-and-its-correlation-as-a-function-for-the-production-of-nutrient-rich-compost
#14
Mukesh Kumar Awasthi, Quan Wang, Hongyu Chen, Meijing Wang, Xiuna Ren, Junchao Zhao, Jiao Li, Di Guo, Dong-Sheng Li, Sanjeev Kumar Awasthi, Xining Sun, Zengqiang Zhang
The influence of biochar amended dewatered fresh sewage sludge (DFSS)-wheat straw co-composting on nutrients transformation and end products quality was investigated. This is the first study to examine the biochar applied compost quality with different kgha(-1) TKN on Brassica rapa L. growth. Seven mixtures were composted over 8-weeks period in 130-L reactor using the same DFSS with different concentration of biochar (2%, 4%, 6%, 8%, 12% and 18% on dry weight basis) and without additive added treatment served as control...
January 26, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28158841/cold-acclimation-alters-dna-methylation-patterns-and-confers-tolerance-to-heat-and-increases-growth-rate-in-brassica-rapa
#15
Tongkun Liu, Ying Li, Weike Duan, Feiyi Huang, Xilin Hou
No abstract text is available yet for this article.
February 1, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28154137/biosynthesis-of-cabbage-phytoalexins-from-indole-glucosinolate
#16
Andrew P Klein, Elizabeth S Sattely
Brassica crop species are prolific producers of indole-sulfur phytoalexins that are thought to have an important role in plant disease resistance. These molecules are conspicuously absent in the model plant Arabidopsis thaliana, and little is known about the enzymatic steps that assemble the key precursor brassinin. Here, we report the minimum set of biosynthetic genes required to generate cruciferous phytoalexins starting from the well-studied glucosinolate pathway. In vitro biochemical characterization revealed an additional role for the previously described carbon-sulfur lyase SUR1 in processing cysteine-isothiocyanate conjugates, as well as the S-methyltransferase DTCMT that methylates the resulting dithiocarbamate, together completing a pathway to brassinin...
February 2, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28145061/chemical-variability-and-biological-activities-of-brassica-rapa-var-rapifera-parts-essential-oils-depending-on-geographic-variation-and-extraction-technique
#17
Boualem Saka, Abderrahmane Djouahri, Zineb Djerrad, Terfi Souhila, Sihem Aberrane, Nasserdine Sabaou, Aoumeur Baaliouamer, Lynda Boudarene
In the present work, the Brassica rapa var. rapifera parts essential oils and their antioxidant and antimicrobial activities were investigated for the first time depending on geographic origin and extraction technique. GC and GC-MS analyses showed several constituents, including alcohols, aldehydes, esters, ketones, norisoprenoids, terpenic, nitrogen and sulphur compounds, totalizing 38 and 41 compounds in leaves and root essential oils, respectively. Nitrogen compounds were the main volatiles in leaves essential oils and sulphur compounds were the main volatiles in root essential oils...
February 1, 2017: Chemistry & Biodiversity
https://www.readbyqxmd.com/read/28134434/herbivore-induced-plant-volatiles-accurately-predict-history-of-coexistence-diet-breadth-and-feeding-mode-of-herbivores
#18
Holger Danner, Gaylord A Desurmont, Simona M Cristescu, Nicole M van Dam
Herbivore-induced plant volatiles (HIPVs) serve as specific cues to higher trophic levels. Novel, exotic herbivores entering native foodwebs may disrupt the infochemical network as a result of changes in HIPV profiles. Here, we analysed HIPV blends of native Brassica rapa plants infested with one of 10 herbivore species with different coexistence histories, diet breadths and feeding modes. Partial least squares (PLS) models were fitted to assess whether HIPV blends emitted by Dutch B. rapa differ between native and exotic herbivores, between specialists and generalists, and between piercing-sucking and chewing herbivores...
January 30, 2017: New Phytologist
https://www.readbyqxmd.com/read/28131821/the-fate-of-retrotransposed-processed-genes-in-arabidopsis-thaliana
#19
Basma T M Abdelkarim, Vincent Maranda, Guy Drouin
Processed genes are functional genes that have arisen as a result of the retrotransposition of mRNA molecules. We found 6 genes that generated processed genes in the common ancestor of five Brassicaceae species (Arabidopsis thaliana, Arabidopsis lyrata, Capsella rubella, Brassica rapa and Thellungiella parvula). These processed genes have therefore been kept for at least 30millionyears. Analyses of the Ka/Ks ratio of these genes, and of those having given rise to them, show that they evolve relatively slowly and suggest that the processed genes maintained the same function as that of their parental gene...
April 20, 2017: Gene
https://www.readbyqxmd.com/read/28131477/advances-on-the-determination-of-thresholds-of-cu-phytotoxicity-in-field-contaminated-soils-in-central-chile
#20
Pedro Mondaca, Joanie Catrin, José Verdejo, Sébastien Sauvé, Alexander Neaman
To better determine phytotoxicity thresholds for metals in the soil, studies should use actual field-contaminated soil samples rather than metal-spiked soil preparations. However, there are surprisingly few such data available for Cu phytotoxicity in field-contaminated soils. Moreover, these studies differ from each other with regards to soil characteristics and experimental setups. This study aimed at more accurately estimating Cu phytotoxicity thresholds using field-collected agricultural soils (Entisols) from areas exposed to contamination from Cu mining...
April 2017: Environmental Pollution
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