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Brassica rapa

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https://www.readbyqxmd.com/read/28799009/genome-wide-survey-and-characterization-of-fatty-acid-desaturase-gene-family-in-brassica-napus-and-its-parental-species
#1
Yufei Xue, Baojun Chen, Rui Wang, Aung Naing Win, Jiana Li, Yourong Chai
Rapeseed (Brassica napus) is an important oilseed crop worldwide, and fatty acid (FA) compositions determine the nutritional and economic value of its seed oil. Fatty acid desaturases (FADs) play a pivotal role in regulating FA compositions, but to date, no comprehensive genome-wide analysis of FAD gene family in rapeseed and its parent species has been reported. In this study, using homology searches, 84, 45, and 44 FAD genes were identified in rapeseed, Brassica rapa, and Brassica oleracea genomes, respectively...
August 10, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28797073/assessment-of-veterinary-drugs-in-plants-using-pharmacokinetic-approaches-the-absorption-distribution-and-elimination-of-tetracycline-and-sulfamethoxazole-in-ephemeral-vegetables
#2
Hui-Ru Chen, Tirawat Rairat, Shih-Hurng Loh, Yu-Chieh Wu, Thomas W Vickroy, Chi-Chung Chou
The present study was carried out to demonstrate novel use of pharmacokinetic approaches to characterize drug behaviors/movements in the vegetables with implications to food safety. The absorption, distribution, metabolism and most importantly, the elimination of tetracycline (TC) and sulfamethoxazole (SMX) in edible plants Brassica rapa chinensis and Ipomoea aquatica grown hydroponically were demonstrated and studied using non-compartmental pharmacokinetic analysis. The results revealed drug-dependent and vegetable-dependent pharmacokinetic differences and indicated that ephemeral vegetables could have high capacity accumulating antibiotics (up to 160 μg g-1 for TC and 38 μg g-1 for SMX) within hours...
2017: PloS One
https://www.readbyqxmd.com/read/28789614/comparative-transcriptome-analysis-reveals-key-cadmium-transport-related-genes-in-roots-of-two-pak-choi-brassica-rapa-l-ssp-chinensis-cultivars
#3
Rugang Yu, Dan Li, Xueling Du, Shenglan Xia, Caifeng Liu, Gangrong Shi
BACKGROUND: Cadmium translocation from roots to shoots is a complex biological process that is controlled by gene regulatory networks. Pak choi exhibits wide cultivar variations in Cd accumulation. However, the molecular mechanism involved in cadmium translocation and accumulation is still unclear. To isolate differentially expressed genes (DEGs) involved in transporter-mediated regulatory mechanisms of Cd translocation in two contrasting pak choi cultivars, Baiyewuyueman (B, high Cd accumulator) and Kuishan'aijiaoheiye (K, low Cd accumulator), eight cDNA libraries from the roots of two cultivars were constructed and sequenced by RNA-sequencing...
August 8, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28767102/allocation-to-male-vs-female-floral-function-varies-by-currency-and-responds-differentially-to-density-and-moisture-stress
#4
M T Brock, R L Winkelman, M J Rubin, C E Edwards, B E Ewers, C Weinig
Allocation of finite resources to separate reproductive functions is predicted to vary across environments and affect fitness. Biomass is the most commonly measured allocation currency; however, in comparison with nutrients it may be less limited and express different environmental and evolutionary responses. Here, we measured carbon, nitrogen, phosphorus, and biomass allocation among floral whorls in recombinant inbred lines of Brassica rapa in multiple environments to characterize the genetic architecture of floral allocation, including its sensitivity to environmental heterogeneity and to choice of currency...
August 2, 2017: Heredity
https://www.readbyqxmd.com/read/28751801/drought-tolerant-brassica-rapa-shows-rapid-expression-of-gene-networks-for-general-stress-responses-and-programmed-cell-death-under-simulated-drought-stress
#5
Yi Ming Guo, Birgit Samans, Sheng Chen, Kidist B Kibret, Sarah Hatzig, Neil C Turner, Matthew N Nelson, Wallace A Cowling, Rod J Snowdon
Production of oilseed rape/canola (Brassica napus) is increasingly threatened by dry conditions while the demand for vegetable oil is increasing. Brassica rapa is a genetically diverse ancestor of B. napus, and is readily crossed with B. napus. Recently, we reported promising levels of drought tolerance in a wild type of B. rapa which could be a source of drought tolerance for B. napus. We analysed global gene expression by messenger RNA sequencing in seedlings of the drought-tolerant and a drought-sensitive genotype of B...
2017: Plant Molecular Biology Reporter
https://www.readbyqxmd.com/read/28744174/analysis-of-cold-resistance-and-identification-of-ssr-markers-linked-to-cold-resistance-genes-in-brassica-rapa-l
#6
Zhen Huang, Xuexian Zhang, Shouhua Jiang, Mengfan Qin, Na Zhao, Lina Lang, Yaping Liu, Zhengshu Tian, Xia Liu, Yang Wang, Binbin Zhang, Aixia Xu
Currently, cold temperatures are one of the main factors threatening rapeseed production worldwide; thus, it is imperative to identify cold-resistant germplasm and to cultivate cold-resistant rapeseed varieties. In this study, the cold resistance of four Brassica rapa varieties was analyzed. The cold resistance of Longyou6 and Longyou7 was better than that of Tianyou2 and Tianyou4. Thus, an F2 population derived from Longyou6 and Tianyou4 was used to study the correlation of cold resistance and physiological indexes...
June 2017: Breeding Science
https://www.readbyqxmd.com/read/28743951/genome-wide-analysis-and-expression-divergence-of-the-trihelix-family-in-brassica-rapa-insight-into-the-evolutionary-patterns-in-plants
#7
Wenli Wang, Peng Wu, TongKong Liu, Haibo Ren, Ying Li, Xilin Hou
Trihelix gene family is an important transcription factor (TF) family involved in plants' growth and development. This extensive study of trihelix genes from Arabidopsis thaliana to Brassica rapa could shed light on the evolution in plants and support crop breeding. In this study, a total of 52 trihelix genes were identified in B.rapa. Whole-genome annotation, molecular-evolution and gene-expression analyses of all known trihelix genes were conducted. By statistics of the number of trihelix genes in each species, we found the expansion of trihelix gene family started with angiosperm evolution...
July 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28736570/genome-wide-identification-and-functional-analysis-of-the-calcineurin-b-like-protein-and-calcineurin-b-like-protein-interacting-protein-kinase-gene-families-in-turnip-brassica-rapa-var-rapa
#8
Xin Yin, Qiuli Wang, Qian Chen, Nan Xiang, Yunqiang Yang, Yongping Yang
The calcineurin B-like protein (CBL)-CBL-interacting protein kinase (CIPK) complex has been identified as a primary component in calcium sensors that perceives various stress signals. Turnip (Brassica rapa var. rapa) has been widely cultivated in the Qinghai-Tibet Plateau for a century as a food crop of worldwide economic significance. These CBL-CIPK complexes have been demonstrated to play crucial roles in plant response to various environmental stresses. However, no report is available on the genome-wide characterization of these two gene families in turnip...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28732175/genome-wide-identification-functional-prediction-and-evolutionary-analysis-of-r2r3-myb-superfamily-in-brassica-napus
#9
Ali Hajiebrahimi, Hajar Owji, Shiva Hemmati
R2R3-MYBs are important transcription factors (TFs) in planta, involved in development and various stress conditions. Phylogenetic analysis showed the presence of 249 R2R3-MYB TFs in Brassica napus called BnaR2R3-MYBs, clustered into 38 clades. BnaR2R3-MYBs were distributed on 19 chromosomes of B. napus. Sixteen gene clusters were identified. BnaR2R3-MYBs were characterized by motif prediction, gene structure analysis and gene ontology. Evolutionary analysis revealed that BnaR2R3-MYBs are mainly formed as a result of whole genome duplication...
July 21, 2017: Genome Génome / Conseil National de Recherches Canada
https://www.readbyqxmd.com/read/28731514/with-or-without-you-effects-of-the-concurrent-range-expansion-of-an-herbivore-and-its-natural-enemy-on-native-species-interactions
#10
David Carrasco, Gaylord A Desurmont, Diane Laplanche, Magali Proffit, Rieta Gols, Paul G Becher, Mattias C Larsson, Ted C J Turlings, Peter Anderson
Global climatic changes may lead to the arrival of multiple range-expanding species from different trophic levels into new habitats, either simultaneously or in quick succession, potentially causing the introduction of manifold novel interactions into native food webs. Unraveling the complex biotic interactions between native and range-expanding species is critical to understand the impact of climate change on community ecology, but experimental evidence is lacking. In a series of laboratory experiments that simulated direct and indirect species interactions, we investigated the effects of the concurrent arrival of a range-expanding insect herbivore in Europe, Spodoptera littoralis, and its associated parasitoid Microplitis rufiventris, on the native herbivore Mamestra brassicae, and its associated parasitoid M...
July 21, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28729817/ssr-marker-variations-in-brassica-species-provide-insight-into-the-origin-and-evolution-of-brassica-amphidiploids
#11
Ajay Kumar Thakur, Kunwar Harendra Singh, Lal Singh, Joghee Nanjundan, Yasin Jeshima Khan, Dhiraj Singh
BACKGROUND: Oilseed Brassica represents an important group of oilseed crops with a long history of evolution and cultivation. To understand the origin and evolution of Brassica amphidiploids, simple sequence repeat (SSR) markers were used to unravel genetic variations in three diploids and three amphidiploid Brassica species of U's triangle along with Eruca sativa as an outlier. RESULTS: Of 124 Brassica-derived SSR loci assayed, 100% cross-transferability was obtained for B...
2018: Hereditas
https://www.readbyqxmd.com/read/28710130/dead-or-alive-using-membrane-failure-and-chlorophyll-fluorescence-to-predict-mortality-from-drought
#12
Carmela Rosaria Guadagno, Brent E Ewers, Heather N Speckman, Timothy L Aston, Bridger J Huhn, Stanley B DeVore, Joshua T Ladwig, Rachel N Strawn, Cynthia Weinig
Climate models predict widespread increases in both drought intensity and duration in the next decades. Although water deficiency is one of the greatest environmental pressures to plant survival, limited understanding of how plants respond to extreme drought impedes adequate forecasts of how increasing aridity will impact both forest and crop productivity. Drought induces a suite of physiological responses; however, we lack an accurate mechanistic description of plant response to lethal drought that would improve predictive understanding of the timing of lethal drought with climate change...
July 14, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28694825/rapid-separation-of-indole-glucosinolates-in-roots-of-chinese-cabbage-brassica-rapa-subsp-pekinensis-by-high-performance-liquid-chromatography-with-diode-array-detection
#13
Alfredo Aires, Rosa Carvalho
Glucosinolates are a class of sulphur-containing plant compounds with diverse biological properties. They have been found exclusively in the Brassicaceae family plants and studied exhaustively in biosynthetic and application perspectives. The aim of this current study is to present a simple and updated method to quantify indole glucosinolate content in hairy root cultures of Chinese cabbage by HPLC-DAD-UV/Vis. Method validation controls were performed and recovery experiments were assayed. The data was statically treated and compared with published works...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28680265/rapanet-a-web-tool-for-the-co-expression-analysis-of-brassica-rapa-genes
#14
Jiye Kim, Kyong Mi Jun, Joung Sug Kim, Songhwa Chae, Yoon-Mok Pahk, Tae-Ho Lee, Soo-In Sohn, Soo In Lee, Myung-Ho Lim, Chang-Kug Kim, Yoonkang Hur, Baek Hie Nahm, Yeon-Ki Kim
Accumulated microarray data are used for assessing gene function by providing statistical values for co-expressed genes; however, only a limited number of Web tools are available for analyzing the co-expression of genes of Brassica rapa. We have developed a Web tool called RapaNet (http://bioinfo.mju.ac.kr/arraynet/brassica300k/query/), which is based on a data set of 143 B rapa microarrays compiled from various organs and at different developmental stages during exposure to biotic or abiotic stress. RapaNet visualizes correlated gene expression information via correlational networks and phylogenetic trees using Pearson correlation coefficient (r)...
2017: Evolutionary Bioinformatics Online
https://www.readbyqxmd.com/read/28674416/genotyping-by-sequencing-reveals-three-qtl-for-clubroot-resistance-to-six-pathotypes-of-plasmodiophora-brassicae-in-brassica-rapa
#15
Fengqun Yu, Xingguo Zhang, Gary Peng, Kevin C Falk, Stephen E Strelkov, Bruce D Gossen
Clubroot, caused by Plasmodiophora brassicae, is an important disease of Brassica crops worldwide. F1 progeny from the Brassica rapa lines T19 (resistant) × ACDC (susceptible) were backcrossed with ACDC, then self-pollinated to produce BC1S1 lines, From genotyping-by-sequencing (GBS) of the parental lines and BC1 plants, about 1.32 M sequences from T19 were aligned into the reference genome of B. rapa with 0.4-fold coverage, and 1.77 M sequences with 0.5-fold coverage in ACDC. The number of aligned short reads per plant in the BC1 ranged from 0...
July 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28670794/brassica-rapa-hairy-root-extracts-promote-skin-depigmentation-by-modulating-melanin-production-and-distribution
#16
Luigi Michele Sena, Claudia Zappelli, Fabio Apone, Ani Barbulova, Annalisa Tito, Antonella Leone, Teresa Oliviero, Rosalia Ferracane, Vincenzo Fogliano, Gabriella Colucci
BACKGROUND: Skin whitening products, used for ages by Asian people for cultural and esthetic purposes, are very popular nowadays in Western countries as well, where the need to inhibit skin spots after sun exposure has become not only a cosmetic but also a health-related issue. Thus, the development of effective and safe depigmenting agents derived from natural products gets continuous attention by cosmetic brands and consumers. OBJECTIVES: The aim of this study was to determine the effects of two preparations, obtained from the hairy root cultures of the species Brassica rapa, on melanogenesis and the expression of the extracellular matrix proteins involved in a correct pigment distribution...
July 3, 2017: Journal of Cosmetic Dermatology
https://www.readbyqxmd.com/read/28650458/duplicated-pollen-pistil-recognition-loci-control-intraspecific-unilateral-incompatibility-in-brassica-rapa
#17
Yoshinobu Takada, Kohji Murase, Hiroko Shimosato-Asano, Takahiro Sato, Honoka Nakanishi, Keita Suwabe, Kentaro K Shimizu, Yong Pyo Lim, Seiji Takayama, Go Suzuki, Masao Watanabe
In plants, cell-cell recognition is a crucial step in the selection of optimal pairs of gametes to achieve successful propagation of progeny. Flowering plants have evolved various genetic mechanisms, mediated by cell-cell recognition, to enable their pistils to reject self-pollen, thus preventing inbreeding and the consequent reduced fitness of progeny (self-incompatibility, SI), and to reject foreign pollen from other species, thus maintaining species identity (interspecific incompatibility)(1). In the genus Brassica, the SI system is regulated by an S-haplotype-specific interaction between a stigma-expressed female receptor (S receptor kinase, SRK) and a tapetum cell-expressed male ligand (S locus protein 11, SP11), encoded by their respective polymorphic genes at the S locus(2-6)...
June 26, 2017: Nature Plants
https://www.readbyqxmd.com/read/28647368/identification-and-characterization-of-the-leaf-specific-networks-of-inner-and-rosette-leaves-in-brassica-rapa
#18
Man-Sun Kim, Seongmin Hong, Sangeeth Prasath Devaraj, Subin Im, Jeong-Rae Kim, Yong Pyo Lim
Inner and rosette leaves of Chinese cabbage (Brassica rapa) have different characteristics in terms of nutritional value, appearance, taste, color and texture. Many researchers have utilized differentially expressed genes for exploring the difference between inner and rosette leaves of Brassica rapa. The functional characteristics of a gene, however, is determined by complex interactions between genes. Hence, a noble network approach is required for elucidating such functional difference that is not captured by gene expression profiles alone...
August 26, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28645261/genome-wide-analysis-of-udp-glycosyltransferase-super-family-in-brassica-rapa-and-brassica-oleracea-reveals-its-evolutionary-history-and-functional-characterization
#19
Jingyin Yu, Fan Hu, Komivi Dossa, Zhaokai Wang, Tao Ke
BACKGROUND: Glycosyltransferases comprise a highly divergent and polyphyletic multigene family that is involved in widespread modification of plant secondary metabolites in a process called glycosylation. According to conserved domains identified in their amino acid sequences, these glycosyltransferases can be classified into a single UDP-glycosyltransferase (UGT) 1 superfamily. RESULTS: We performed genome-wide comparative analysis of UGT genes to trace evolutionary history in algae, bryophytes, pteridophytes, and angiosperms; then, we further investigated the expansion mechanisms and function characterization of UGT gene families in Brassica rapa and Brassica oleracea...
June 23, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28640193/identification-of-light-independent-anthocyanin-biosynthesis-mutants-induced-by-ethyl-methane-sulfonate-in-turnip-tsuda-brassica-rapa
#20
Jian-Fei Yang, Yun-Zhu Chen, Saneyuki Kawabata, Yu-Hua Li, Yu Wang
The epidermis of swollen storage roots in purple cultivars of turnip "Tsuda" (Brassica rapa) accumulates anthocyanin in a light-dependent manner, especially in response to UV-A light, of which the mechanism is unclear. In this study, we mutagenized 15,000 seeds by 0.5% (v/v) ethyl methane sulfonate (EMS) and obtained 14 mutants with abnormal anthocyanin production in their epidermis of swollen storage roots. These mutants were classified into two groups: the red mutants with constitutive anthocyanin accumulation in their epidermis of storage roots even in underground parts in darkness and the white mutants without anthocyanin accumulation in the epidermis of storage roots in aboveground parts exposed to sunlight...
June 22, 2017: International Journal of Molecular Sciences
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