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https://www.readbyqxmd.com/read/29330901/floral-contrivances-and-specialized-pollination-mechanism-confer-strong-influence-to-elicit-mixed-mating-in-wrightia-tomentosa-apocynaceae
#1
Chandan Barman, Vineet Kumar Singh, Sandip Das, Rajesh Tandon
• Reproductive success of a plant species is largely influenced by the outcomes of mating pattern in a population. It is believed that a significantly greater proportion of animal-pollinated plants have evolved a mixed-mating strategy, the extent of which may vary among species. It is thus pertinent to investigate the key contributors of mating success, especially to identify the reproductive constraints in depauperated populations of threatened plant species. • We looked into the contribution of floral architecture, pollination mechanism and breeding system on the extent of outcrossing rate in a near threatened tree species, W...
January 13, 2018: Plant Biology
https://www.readbyqxmd.com/read/29330459/a-novel-ultra-stable-monomeric-green-fluorescent-protein-for-direct-volumetric-imaging-of-whole-organs-using-clarity
#2
Daniel J Scott, Natalie J Gunn, Kelvin J Yong, Verena C Wimmer, Nicholas A Veldhuis, Leesa M Challis, Mouna Haidar, Steven Petrou, Ross A D Bathgate, Michael D W Griffin
Recent advances in thick tissue clearing are enabling high resolution, volumetric fluorescence imaging of complex cellular networks. Fluorescent proteins (FPs) such as GFP, however, can be inactivated by the denaturing chemicals used to remove lipids in some tissue clearing methods. Here, we solved the crystal structure of a recently engineered ultra-stable GFP (usGFP) and propose that the two stabilising mutations, Q69L and N164Y, act to improve hydrophobic packing in the core of the protein and facilitate hydrogen bonding networks at the surface, respectively...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321878/asymmetric-reproductive-interference-the-consequences-of-cross-pollination-on-reproductive-success-in-sexual-apomictic-populations-of-potentilla-puberula-rosaceae
#3
Christoph Dobeš, Susanne Scheffknecht, Yulia Fenko, Dagmar Prohaska, Christina Sykora, Karl Hülber
Apomixis evolves from a sexual background and usually is linked to polyploidization. Pseudogamous gametophytic apomicts, which require a fertilization to initiate seed development, of various ploidy levels frequently co-occur with their lower-ploid sexual ancestors, but the stability of such mixed populations is affected by reproductive interferences mediated by cross-pollination. Thereby, reproductive success of crosses depends on the difference in ploidy levels of mating partners, that is, on tolerance of deviation from the balanced ratio of maternal versus paternal genomes...
January 2018: Ecology and Evolution
https://www.readbyqxmd.com/read/29321124/hearing-tests-based-on-biologically-calibrated-mobile-devices-comparison-with-pure-tone-audiometry
#4
Marcin Masalski, Tomasz Grysiński, Tomasz Kręcicki
BACKGROUND: Hearing screening tests based on pure-tone audiometry may be conducted on mobile devices, provided that the devices are specially calibrated for the purpose. Calibration consists of determining the reference sound level and can be performed in relation to the hearing threshold of normal-hearing persons. In the case of devices provided by the manufacturer, together with bundled headphones, the reference sound level can be calculated once for all devices of the same model. OBJECTIVE: This study aimed to compare the hearing threshold measured by a mobile device that was calibrated using a model-specific, biologically determined reference sound level with the hearing threshold obtained in pure-tone audiometry...
January 10, 2018: JMIR MHealth and UHealth
https://www.readbyqxmd.com/read/29297326/advantages-of-amplifluor-like-snp-markers-over-kasp-in-plant-genotyping
#5
Satyvaldy Jatayev, Akhylbek Kurishbayev, Lyudmila Zotova, Gulmira Khasanova, Dauren Serikbay, Askar Zhubatkanov, Makpal Botayeva, Aibek Zhumalin, Arysgul Turbekova, Kathleen Soole, Peter Langridge, Yuri Shavrukov
BACKGROUND: KASP (KBioscience Competitive Allele Specific PCR) and Amplifluor (Amplification with fluorescence) SNP markers are two prominent technologies based upon a shared identical Allele-specific PCR platform. METHODS: Amplifluor-like SNP and KASP analysis was carried out using published and own design of Universal probes (UPs) and Gene-specific primers (GSPs). RESULTS: Advantages of the Amplifluor-like system over KASP include the significantly lower costs and much greater flexibility in the adjustment and development of 'self-designed' dual fluorescently-labelled UPs and regular GSPs...
December 28, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29297221/amphiphilic-diblock-fullerene-derivatives-as-cathode-interfacial-layer-for-organic-solar-cells
#6
Jikang Liu, Junli Li, Xiangfu Liu, Fu Li, Guoli Tu
A new amphiphilic diblock fullerene derivative [6,6]-Phenyl-C61-butyricacid-4-(9,9,9',9'-tetrakis(3-bromopropyl)-9H,9'H-[2,2'-bifluoren]-7-yl)phenol-(N,N,N-trimethylpropan-1-aminium) bromide (C60-4TPB), was synthesized and applied in Organic Solar Cells (OSCs). Solvent annealing by toluene could obvious induce the self-assembly of the C60-4TPB layer, which can be tested by the measurements of the water contact angle. After the treatment of toluene, a vertical like arrangement in the ultrathin layer of C60-4TPB molecular will be formed between electron-collecting zinc oxide (ZnO) layers and the active layer (blend system of PTB7/PC71BM), leading to the improvement of the interfacial compatibility between active layer and ZnO layer...
January 3, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29289308/creasensor-simple-technology-for-creatinine-detection-in-plasma
#7
Francesco Dal Dosso, Deborah Decrop, Elena Pérez-Ruiz, Devin Daems, Hannah Agten, Osamah Al-Ghezi, Olivier Bollen, Jolien Breukers, Florian De Rop, Maria Katsafadou, Jens Lepoudre, Linye Lyu, Pieter Piron, Robbe Saesen, Shoera Sels, Rani Soenen, Ellen Staljanssens, Jehan Taraporewalla, Tadej Kokalj, Dragana Spasic, Jeroen Lammertyn
The lab-on-a-chip (LOC) field has witnessed an excess of new technology concepts, especially for the point-of-care (POC) applications. However, only few concepts reached the POC market often because of challenging integration with pumping and detection systems as well as with complex biological assays. Recently, a new technology termed SIMPLE was introduced as a promising POC platform due to its features of being self-powered, autonomous in liquid manipulations, cost-effective and amenable to mass production...
February 13, 2018: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29283472/seed-germination-and-seedling-allogamy-in-rosmarinus-officinalis-the-costs-of-inbreeding
#8
Patricio Garcia-Fayos, Maria Clara Castellanos, José Gabriel Segarra-Moragues
1.Self-pollination by geitonogamy is likely in self-compatible plants that simultaneously expose large numbers of flowers to pollinators. However, the progeny of these plants is often highly allogamous. Although mechanisms to increase cross-pollination have been identified and studied, their relative importance has rarely been addressed simultaneously in plant populations. 2.We used Rosmarinus officinalis to explore the factors that influence the probability of self-fertilization due to geitonogamy or that purge its consequences, focusing on their effect on seed germination and allogamy rate...
December 28, 2017: Plant Biology
https://www.readbyqxmd.com/read/29281736/characterizing-first-and-third-person-viewpoints-and-their-alternation-for-embodied-interaction-in-virtual-reality
#9
Henrique Galvan Debarba, Sidney Bovet, Roy Salomon, Olaf Blanke, Bruno Herbelin, Ronan Boulic
Empirical research on the bodily self has shown that the body representation is malleable, and prone to manipulation when conflicting sensory stimuli are presented. Using Virtual Reality (VR) we assessed the effects of manipulating multisensory feedback (full body control and visuo-tactile congruence) and visual perspective (first and third person perspective) on the sense of embodying a virtual body that was exposed to a virtual threat. We also investigated how subjects behave when the possibility of alternating between first and third person perspective at will was presented...
2017: PloS One
https://www.readbyqxmd.com/read/29277938/tools-of-the-crook-infection-strategies-of-fungal-plant-pathogens
#10
Luis Rodriguez-Moreno, Malaika K Ebert, Melvin D Bolton, Bart P H J Thomma
Fungi represent an ecologically diverse group of microorganisms that includes plant pathogenic species able to cause considerable yield loses in crop production systems worldwide. In order to establish compatible interactions with their hosts, pathogenic fungi rely on the secretion of molecules of diverse nature during host colonization to modulate host physiology, manipulate other environmental factors or provide self-defence. These molecules, collectively known as effectors, are typically small secreted cysteine-rich proteins, but may also comprise secondary metabolites and sRNAs...
December 26, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29276160/management-and-outcome-of-reduced-fetal-movements-is-ultrasound-necessary
#11
Nasser Abu Awad, Thomas Jordan, Robert Mundle, Dan Farine
OBJECTIVE: To review the management and outcome of pregnancies of women presenting to obstetrical triage with decreased fetal movements (DFM). STUDY DESIGN: A retrospective review of women presenting with DFMs to two large Canadian obstetrical centres with a combined 9490 deliveries per year. The charts were reviewed for compliance with the Canadian guidelines for demographics (age, parity, GA, comorbidities, etc.), pregnancy management (admission vs. discharge, need to deliver), and pregnancy outcomes (mortality, morbidity, GA at delivery, Apgar scores, etc...
December 21, 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/29251457/efficacy-of-an-experimental-3-potassium-nitrate-mouthwash-in-providing-long-term-relief-from-dentin-hypersensitivity-an-8-week-randomized-controlled-study-study-2
#12
Claire Hall, Farzana Sufi, Paul Constantin
PURPOSE: To evaluate the efficacy of an experimental mouthwash containing 3% potassium nitrate (KNO3) in the relief of dentin hypersensitivity when used as an adjunct to brushing with fluoride toothpaste compared with the use of the same toothpaste alone. METHODS: This was a randomized, two-treatment, examiner-blind, parallel-design single-center, 8-week study in healthy subjects with self-reported and clinically diagnosed dentin hypersensitivity. Subjects were randomized to receive either fluoride toothpaste plus 3...
December 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29250859/multitemperature-responsive-self-folding-soft-biomimetic-structures
#13
Kunihiko Kobayashi, Seung Hyun Oh, ChangKyu Yoon, David H Gracias
Untethered, millimeter-scale, stimuli-responsive shape change structures are critical to the function of autonomous devices, smart materials, and soft robotics. Temperature in a range compatible with physiological or ambient environmental conditions is an excellent cue to trigger actuation of soft structures for practical biomimetic applications. Previously, a range of thermally responsive self-folding soft structures has been described and utilized in a variety of applications from tissue engineering to minimally invasive surgery...
December 18, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/29247258/fine-mapping-a-self-fertility-locus-in-perennial-ryegrass
#14
Javier Do Canto, Bruno Studer, Ursula Frei, Thomas Lübberstedt
A self-fertility locus was fine mapped to a 1.6 cM region on linkage group 5 in a perennial ryegrass population. This locus was the main determinant of pollen self-compatibility. In grasses, self-incompatibility (SI) is characterized by a two-loci gametophytic (S and Z) mechanism acting together in the recognition and inhibition of self-pollen. Mutations affecting the expression of SI have been reported in a few grass species. In perennial ryegrass (Lolium perenne L.), a mutation independent from S and Z, and mapping on linkage group 5 (LG 5), was previously reported to produce self-fertile plants...
December 15, 2017: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/29242993/promotion-of-neuronal-regeneration-by-using-self-polymerized-dendritic-polypeptide-scaffold-for-spinal-cord-tissue-engineering
#15
Jun Ming Wan, Liang le Liu, Jian Fang Zhang, Jian Wei Lu, Qi Li
Tissue engineering technology is applicable for study of nerve regeneration after spinal cord injury. Many natural and artificial scaffold are not applicable because of poor mechanical properties and cell compatibility. Polypeptides with fine three-dimensional structure and cell compatibility and are widely used in tissue engineering research. The purpose of this study was to verify the neuronal differentiation of neural stem cells by using self-polymerize dendritic polypeptide for spinal cord tissue engineering...
December 14, 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/29240711/nad1-controls-defense-like-responses-in-medicago-truncatula-symbiotic-nitrogen-fixing-nodules-following-rhizobial-colonization-in-a-baca-independent-manner
#16
Ágota Domonkos, Szilárd Kovács, Anikó Gombár, Ernő Kiss, Beatrix Horváth, Gyöngyi Z Kováts, Attila Farkas, Mónika T Tóth, Ferhan Ayaydin, Károly Bóka, Lili Fodor, Pascal Ratet, Attila Kereszt, Gabriella Endre, Péter Kaló
Legumes form endosymbiotic interaction with host compatible rhizobia, resulting in the development of nitrogen-fixing root nodules. Within symbiotic nodules, rhizobia are intracellularly accommodated in plant-derived membrane compartments, termed symbiosomes. In mature nodule, the massively colonized cells tolerate the existence of rhizobia without manifestation of visible defense responses, indicating the suppression of plant immunity in the nodule in the favur of the symbiotic partner. Medicago truncatulaDNF2 (defective in nitrogen fixation 2) and NAD1 (nodules with activated defense 1) genes are essential for the control of plant defense during the colonization of the nitrogen-fixing nodule and are required for bacteroid persistence...
December 14, 2017: Genes
https://www.readbyqxmd.com/read/29239524/performance-measures-for-a-dialysis-setting
#17
Xiuzhu Gu, Kenji Itoh
OBJECTIVES: This study from Japan extracted performance measures for dialysis unit management and investigated their characteristics from professional views. METHODS: Two surveys were conducted using self-administered questionnaires, in which dialysis managers/staff were asked to rate the usefulness of 44 performance indicators. A total of 255 managers and 2,097 staff responded. RESULTS: Eight performance measures were elicited from dialysis manager and staff responses: these were safety, operational efficiency, quality of working life, financial effectiveness, employee development, mortality, patient/employee satisfaction and patient-centred health care...
December 14, 2017: Journal of Renal Care
https://www.readbyqxmd.com/read/29238503/designing-and-validation-a-questionnaire-of-hope-for-family-members-of-patients-hospitalized-in-intensive-care-unit
#18
Foad Rahimi, Fatemeh Oskouie, Abbas Ebadi
Background: Families of patients are faced with stressors in the intensive care unit (ICU) that cannot be controlled sometimes. Having hope is one of the strategies for obtaining self-control in patients' families, which deals with such situations. Objective: The purpose of this study was to design and validate a questionnaire of hope for family members of patients hospitalized in intensive care units. Methods: This study was conducted for family members of patients hospitalized in the intensive care units of Kurdistan and Tehran provinces, in Iran, between the years 2015-2016...
October 2017: Electronic Physician
https://www.readbyqxmd.com/read/29237581/a-web-based-training-resource-for-therapists-to-deliver-an-evidence-based-exercise-program-for-rheumatoid-arthritis-of-the-hand-isarah-design-development-and-usability-testing
#19
Cynthia Swarnalatha Srikesavan, Esther Williamson, Lucy Eldridge, Peter Heine, Jo Adams, Tim Cranston, Sarah E Lamb
BACKGROUND: The Strengthening and Stretching for Rheumatoid Arthritis of the Hand (SARAH) is a tailored, progressive exercise program for people having difficulties with wrist and hand function due to rheumatoid arthritis (RA). The program was evaluated in a large-scale clinical trial and was found to improve hand function, was safe to deliver, and was cost-effective. These findings led to the SARAH program being recommended in the UK National Institute for Health and Care Excellence guidelines for the management of adults with RA...
December 13, 2017: Journal of Medical Internet Research
https://www.readbyqxmd.com/read/29235842/mussel-inspired-synthesis-of-nir-responsive-and-biocompatible-ag-graphene-2d-nano-agents-for-versatile-bacterial-disinfections
#20
Xin Fan, Fan Yang, Chuanxiong Nie, Ye Yang, Haifeng Ji, Chao He, Chong Cheng, Changsheng Zhao
Pathogenic bacterial infection has been becoming as a global threat towards people's health, especially the massive usage of antibiotics due to the lack of antibacterial agents with less side-effects. Developing new nano-agents to fight pathogenic bacteria has provided enormous new possibilities in the treatment of bacterial infections, such as the graphene based 2D antibacterial nano-agents with different bacterial inhibition capabilities; however, mussel-inspired design of NIR-responsive and biocompatible Ag-graphene nano-agents possessed with efficient and versatile bacterial disinfection activities have rarely been reported...
December 13, 2017: ACS Applied Materials & Interfaces
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