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Kamil Kowalski, Aleksandra Kołodziejczyk, Maria Helena Sikorska, Jagoda Płaczkiewicz, Paulina Cichosz, Magdalena Kowalewska, Wladyslawa Streminska, Katarzyna Janczyk-Ilach, Marta Koblowska, Anna Fogtman, Roksana Iwanicka-Nowicka, Maria A Ciemerych, Edyta Brzoska
The skeletal muscle regeneration occurs due to the presence of tissue specific stem cells - satellite cells. These cells, localized between sarcolemma and basal lamina, are bound to muscle fibers and remain quiescent until their activation upon muscle injury. Due to pathological conditions, such as extensive injury or dystrophy, skeletal muscle regeneration is diminished. Among the therapies aiming to ameliorate skeletal muscle diseases are transplantations of the stem cells. In our previous studies we showed that Sdf-1 (stromal derived factor -1) increased migration of stem cells and their fusion with myoblasts in vitro...
October 13, 2016: Cell Adhesion & Migration
De-Min Ma, Dian-Xi Luo, Jie Zhang
BACKGROUND: More recent studies have revealed that chemokine receptor CXCR7 plays an important role in cancer development. However, little is known about the effect of CXCR7 on the process of gastric cancer cell invasion and angiogenesis. The aim of this study is to investigate the expression of CXCR7 in gastric cancer cell lines and to evaluate the role of CXCR7 in the proliferation, invasion, adhesion, and angiogenesis of gastric cancer cells. METHODS: Real-time PCR and Western blotting were used to examine the mRNA and protein levels of CXCR4 and CXCR7 in five gastric cancer cell lines (HGC-27, MGC-803, BGC-823, SGC-7901, and MKN-28)...
October 6, 2016: World Journal of Surgical Oncology
Shuchen Hsieh, Hui-Jye Chen, Shan-Hui Hsu, Yi-Chun Yang, Cheng-Ming Tang, Mei-Yun Chu, Pei-Ying Lin, Ru-Huei Fu, Mei-Lang Kung, Yun-Wen Chen, Bi-Wen Yeh, Huey-Shan Hung
The ideal characteristics of surface modification on the vascular graft for clinical application would be with excellent hemocompatibility, endothelialization capacity, and anti-restenosis ability. Here, Fourier transform infrared spectroscopy (FTIR), surface enhanced raman spectroscopy (SERS), atomic force microscopy (AFM), contact angle () measurement, and thermogravimetric analyzer (TGA) were used to evaluate the chemical and mechanical properties of collagen-gold nanocomposites (collagen+Au) with 17...
October 7, 2016: ACS Applied Materials & Interfaces
Cheng-Chun Wu, I-Fang Wang, Po-Min Chiang, Liang-Chao Wang, Che-Kun James Shen, Kuen-Jer Tsai
Recent studies reported granulocyte colony-stimulating factor (G-CSF) treatment can improve the cognitive function of Alzheimer's disease (AD) mice, and the mobilized hematopoietic stem cells (HSCs) or bone marrow mesenchymal stem cells (BM-MSCs) are proposed to be involved in this recovery effect. However, the exact role of mobilized HSC/BM-MSC in G-CSF-based therapeutic effects is still unknown. Here, we report that C-X-C chemokine receptor type 4 (CXCR4)/stromal cell-derived factor 1 (SDF-1) chemotaxis was a key mediator in G-CSF-based therapeutic effects, which was involved in the recruitment of repair-competent cells...
October 5, 2016: Molecular Neurobiology
Mateusz Adamiak, Malwina Suszynska, Ahmed Abdel-Latif, Ahmed Abdelbaset-Ismail, Janina Ratajczak, Mariusz Z Ratajczak
Migration and bone marrow (BM) homing of hematopoietic stem progenitor cells (HSPCs) is regulated by several signaling pathways, and here we provide evidence for the involvement in this process of hematopoietic-specific phospholipase C-β2 (PLC-β2). This enzyme is involved in release of intracellular calcium and activation of protein kinase C (PKC). Recently we reported that PLC-β2 promotes mobilization of HSPCs from BM into peripheral blood (PB), and this effect is mediated by the involvement of PLC-β2 in the release of proteolytic enzymes from granulocytes and its role in disintegration of membrane lipid rafts...
October 4, 2016: Stem Cell Reviews
Hai-Lang He, Dan Wang, Jie Tang, Xian-Mei Zhou, Jian-Xin Li, Ling Xu
Lung cancer is the leading cause of cancer-related deaths worldwide. Jin Fu Kang (JFK), an oral liquid prescription of Chinese herbal drugs, has been clinically available for the treatment of non-small cell lung cancer (NSCLC). Lymphangiogenesis is a primary event in the process of cancer development and metastasis, and the formation and migration of lymphatic endothelial cells (LECs) play a key role in the lymphangiogenesis. To assess the activity of stromal cell-derived factor-1 (SDF-1) and the coeffect of SDF-1 and vascular endothelial growth factor-C (VEGF-C) on the formation and migration of LECs and clarify the inhibitory effects of JFK on the LECs, the LECs were differentiated from CD34(+)/VEGFR-3(+) endothelial progenitor cells (EPCs), and JFK-containing serums were prepared from rats...
2016: Evidence-based Complementary and Alternative Medicine: ECAM
Yang-Hee Kim, Yasuhiko Tabata
The objective of the present study is to evaluate the effects of the release patterns of stromal derived factor (SDF)-1 and sphingosine-1 phosphate agonist (SEW2871), used as MSC and macrophage recruitment agents, on the wound closure of diabetic mouse skin defects. To achieve different release patterns, hydrogels were prepared using two types of gelatin with isoelectric points (IEP) of 5 and 9, into which SDF-1 and SEW2871 were then incorporated in various combinations. When the hydrogels incorporating SDF-1 and SEW2871 were applied into wound defects of diabetic mice, the number of MSCs and macrophages recruited to the defects and the levels of pro- and anti- inflammatory cytokines were found to be dependent on the release profiles of SDF-1 and SEW2871...
October 3, 2016: Journal of Tissue Engineering and Regenerative Medicine
Michael B Cheung, Viviana Sampayo-Escobar, Ryan Green, Martin L Moore, Subhra Mohapatra, Shyam S Mohapatra
Respiratory syncytial virus (RSV) has been reported to infect human mesenchymal stem cells (MSCs) but the consequences are poorly understood. MSCs are present in nearly every organ including the nasal mucosa and the lung and play a role in regulating immune responses and mediating tissue repair. We sought to determine whether RSV infection of MSCs enhances their immune regulatory functions and contributes to RSV-associated lung disease. RSV was shown to replicate in human MSCs by fluorescence microscopy, plaque assay, and expression of RSV transcripts...
2016: PloS One
Meerim Park, Chan-Jeoung Park, Young Wook Cho, Seongsoo Jang, Jung-Hee Lee, Je-Hwan Lee, Kyoo-Hyung Lee, Young Ho Lee
Hematopoiesis involves complex interactions between hematopoietic cells and the bone marrow (BM) microenvironment; the specific causes and mechanisms underlying dysregulated hematopoiesis are unknown. Here, BM biopsy specimens from patients with aplastic anemia (AA), chronic myeloid leukemia (CML), and normal marrow were analyzed by semiquantitative immunohistochemistry to determine changes in the hematopoietic stem cell (HSC) compartment and BM microenvironment. HSC levels were lowest in AA and highest in CML...
September 27, 2016: Experimental Hematology
Wen-Shih Huang, Meng-Chiao Hsieh, Cheng-Yi Huang, Yi-Hung Kuo, Shui-Yi Tung, Chien-Heng Shen, Yung-Yu Hsieh, Chih-Chuan Teng, Kam-Fai Lee, Te-Chuan Chen, Ko-Chao Lee, Hsing-Chun Kuo
The stromal cell-derived factor-1 (SDF-1)/CXC receptor 4 (CXCR4) axis plays an important role in tumor angiogenesis and invasiveness in colorectal cancer (CRC) progression. In addition, metastatic CRC remains one of the most difficult human malignancies to treat because of its chemoresistant behavior. However, the mechanism by which correlation occurs between CXCR4 and the clinical response of CRC to chemotherapy remains unknown. We generated chemoresistant cells with increasing doses of oxaliplatin (OXA) and 5-Fluorouracil (5FU) to develop resistance at a clinical dose...
2016: PloS One
Jun Xie, Ran Li, Han Wu, Jianzhou Chen, Guannan Li, Qinhua Chen, Zhonghai Wei, Guixin He, Lian Wang, Albert Ferro, Biao Xu
Endothelial progenitor cells (EPCs) are a subtype of bone marrow-derived progenitor cells. Stromal cell-derived factor 1 (SDF-1)-mediated EPC mobilization from bone marrow to areas of ischemia plays an important role in angiogenesis. Previous studies have reported that advanced glycation endproducts (AGEs), which are important mediators of diabetes-related vascular pathology, may impair EPC migration and homing, but the mechanism is unclear. Syndecan-4 (synd4) is a ubiquitous heparan sulfate proteoglycan receptor on the cell surface, involved in SDF-1-dependent cell migration...
September 24, 2016: Stem Cells
Dangen Yu, Fei Lv, Jianhe Zhang, Hongjie Li
BACKGROUND: Stromal cell-derived factor-1 (SDF-1) expression has been reported to be a predictor of poor clinical symptoms in certain types of cancer. Vascular endothelial growth factor (VEGF) is a well-known factor that mediates the micro-angiogenesis of solid tumors, and SDF-1 mediated expression of VEGF may promote tumor growth and metastasis, resulting in poor clinical outcome. Therefore, we explored the expression levels of SDF-1 and VEGF in patients with osteosarcoma in order to determine the association between their expression levels and unfavorable outcomes...
September 2016: Annals of Clinical and Laboratory Science
Baolei Wu, Lei Wang, Xi Yang, Ming Mao, Chen Ye, Peng Liu, Zihui Yang, Xinjie Yang, Delin Lei, Chenping Zhang
Mesenchymal stem cells (MSCs), which are physiologically maintained in vascular endothelial cell (VEC)-based niches, play a critical role in tissue regeneration. Our previous studies demonstrated that sympathetic denervation could promote MSC mobilization, thereby enhancing bone formation in distraction osteogenesis (DO), a self-tissue engineering for craniofacial and orthopeadic surgeries. However, the mechanisms on how sympathetic neurotransmitter norepinephrine (NE) regulates MSC migration are not well understood...
September 17, 2016: Experimental Cell Research
Lijun Zhang, Qiuhong Hua, Kaiyi Tang, Changjie Shi, Xin Xie, Ru Zhang
G protein-coupled receptors (GPCRs) are involved in many fundamental cellular responses such as growth, death, movement, transcription and excitation. Their roles in human stem cell neural specialization are not well understood. In this study, we aimed to identify GPCRs that may play a role in the differentiation of human embryonic stem cells (hESCs) to neural stem cells (NSCs). Using a feeder-free hESC neural differentiation protocol, we found that the expression of several chemokine receptors changed dramatically during the hESC/NSC transition...
September 9, 2016: Neuroscience
P M Abdul-Muneer, Adriano Andrea Conte, Debanjan Haldar, Mathew Long, Rachel Patel, Vijayalakshmi Santhakumar, Christopher M Overall, Bryan J Pfister
Traumatic brain injury (TBI), even at mild levels, can activate matrix metalloproteinases (MMPs) and the induction of neuroinflammation that can result in blood brain barrier breakdown and neurodegeneration. MMP2 has a significant role in neuroinflammation and neurodegeneration by modulating the chemokine CXCL12 (stromal cell derived factor SDF-1α) signaling pathway and the induction of apoptosis. SDF-1α is responsible for cell proliferation and differentiation throughout the nervous system and is also implicated in various neurodegenerative illnesses...
September 7, 2016: Brain, Behavior, and Immunity
Shih-Ya Tseng, Ting-Hsing Chao, Yi-Heng Li, Chung-Lung Cho
This study investigated the effect of cilostazol on proangiogenesis functions in human early endothelial progenitor cells (EPCs) in vitro and the therapeutic implication of hybrid therapy with cilostazol and human early EPCs in vivo. Cilostazol significantly increased colony-forming units and enhanced differentiation of EPCs toward endothelial lineage. Treatments resulted in antiapoptotic effects and stimulated proliferation and migration and in vitro vascular tube formation through activation of stromal cell-derived factor-1 (SDF-1)/C-X-C chemokine receptor type 4 (CXCR4)/phosphatidylinositol-3 kinase (PI3K)/Akt signaling pathway...
2016: BioMed Research International
Yan Wu, Fang Huang, Xin Zhou, Shaoling Yu, Qingming Tang, Shue Li, Juan Wang, Lili Chen
The use of biological repair in infectious bone defects has been a major challenge for clinicians. With potential for bone regeneration, stem cell therapy could be an effective biological restoration measure for infection-caused bone destruction. In this study, we propose a new stem cell therapy strategy for infectious bone defect repair through systemic transplantation of human dental pulp stem cells (hDPSCs). Hypoxic preconditioning (HP) is thought to be able to enhance duration of survival and therapeutic potency of engrafted stem cells; therefore, we examined the role of HP on hDPSC therapeutic efficacy...
October 2016: Tissue Engineering. Part A
Qiang Ma, Jun-Jie Yang, Hao Zhou, Ying Zhang, Yun-Dai Chen
OBJECTIVE: To investigate the effect of exenatide on chemotactic migration of adipose-derived stem cells (ADSCs) and confirm that Rho GTPase is the downstream effector protein of SDF-1/CXCR-4 migration pathway. METHODS: ADSCs were isolated, cultured, identified by flow cytometry, and induced to differentiate in vitro. RTCA xCELLigence system was used to analyze the effect of exenatide on ADSC proliferation. The effects of exenatide at different concentrations, AMD3100 (CXCR-4 antagonist), and CCG-1423 (Rho GTPase antagonist) on chemotactic migration of ADSCs were tested using Transwell assay...
August 20, 2016: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Y Wang, P Xu, L Qiu, M Zhang, Y Huang, J C Zheng
Cell cycle regulation of neural progenitor cells (NPCs) is an essential process for neurogenesis, neural development, and repair after brain trauma. Stromal cell-derived factor-1 (SDF-1, CXCL12) with its receptors CXCR4 and CXCR7 are well known in regulating the proliferation and survival of NPCs. The effects of CXCL12 on NPCs cell cycle and its associated signaling pathways remain unclear. The cell cycle regulation protein, Cyclin D1, a downstream target of β-catenin, is critical for cell cycle control and may play an important role in NPCs proliferation...
August 29, 2016: Current Molecular Medicine
Xiaofeng Shen, Yanxia Zhang, Yong Gu, Yun Xu, Yong Liu, Bin Li, Liang Chen
In this study, a cell-free bone tissue engineering system based on a silk fibroin (SF)/nano-hydroxyapatite (nHAp) scaffold was developed, in which two bioactive molecules, stromal cell derived factor-1 (SDF-1) and bone morphogenetic protein-2 (BMP-2), were embedded and released in a sequential and controlled manner to facilitate cell recruitment and bone formation, respectively. BMP-2 was initially loaded into SF microspheres, and these BMP-2 containing microspheres were subsequently encapsulated into the SF/nHAp scaffolds, which were successively functionalized with SDF-1 via physical adsorption...
November 2016: Biomaterials
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