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Friederike Remm, Nicolle Kränkel, Daniela Lener, Daniel J Drucker, Sieghart Sopper, Christoph Brenner
Introduction: DPP4 inhibitors (gliptins) are commonly used antidiabetic drugs for the treatment of type 2 diabetes. Gliptins also act in a glucose-independent manner and show vasoregenerative effects. We have shown that gliptins can remarkably accelerate vascular healing after vascular injury. However, the underlying mechanisms remain unclear. Here, we examined potential signaling pathways linking gliptins to enhanced endothelial regeneration. Methods and Results: We used wild-type and GLP1 receptor knockout ( Glp1r -/- ) mice to investigate the underlying mechanisms of gliptin-induced reendothelialization...
2018: Stem Cells International
Antonella Cotoia, Lucia Mirabella, Sabrina Altamura, Rachele Villani, Flavia Marchese, Giuseppe Ferrara, Karim Mariano, Tullo Livio, Gilda Cinnella
BACKGROUND: Sepsis caused by complicated intra-abdominal infection is associated with high mortality. Loss of endothelial barrier integrity, inflammation, and impaired cellular oxygen have been shown to be primary contributors to sepsis. To date, little is known regarding the pathway for the mobilization of endothelial progenitor cells (EPCs) from the bone marrow in sepsis whereas stromal-cell-derived factor 1a (SDF-1a) and hypoxia inducible factor 1 (HIF-1) seem to have a role in the EPC response to hypoxic microenvironments...
March 12, 2018: Trials
Yejun Hu, Jisheng Ran, Zefeng Zheng, Zhangchu Jin, Xiao Chen, Zi Yin, Chenqi Tang, Yangwu Chen, Jiayun Huang, Huihui Le, Ruijian Yan, Ting Zhu, Junjuan Wang, Junxin Lin, Kan Xu, Yiting Zhou, Wei Zhang, Youzhi Cai, Pioletti Dominique, Boon Chin Heng, Weishan Chen, Weiliang Shen, Hong-Wei Ouyang
Anterior cruciate ligament (ACL) is one of the most difficult tissues to heal once injured. Ligament regeneration and tendon-bone junction healing are two major goals of ACL reconstruction. This study aimed to investigate the synergistic therapeutic effects of Stromal cell-derived factor 1(SDF-1)-releasing collagen-silk (CSF) scaffold combined with intra-articular injection of ligament-derived stem/progenitor cells (LSPCs) for ACL regeneration and the amelioration in the long-term complication of osteoarthritis (OA)...
March 7, 2018: Acta Biomaterialia
Zhen Nan, Heng Fan, Qing Tang, Man Zhang, Meng Xu, Qianyun Chen, Yujin Liu, Yalan Dong, Hui Wu, Shuangjiao Deng
Bone marrow-derived mesenchymal stem cells (BMSCs) hold great promise for the treatment of inflammatory bowel disease (IBD) owing to the immunosuppressive property and tissue healing potential. The balance of Treg/Th17 plays a crucial part in BMSC-mediated immunosuppression. Interleukin (IL)-35 is a newly identified anti-inflammatory cytokine required for the expansion of regulatory T cells (Tregs) and suppression of Th17 cells differentiation. It could amplify the immunosuppressive property of BMSCs by transfecting into BMSCs...
March 7, 2018: Biochemical and Biophysical Research Communications
Klara Janjić, Barbara Cvikl, Christoph Kurzmann, Andreas Moritz, Hermann Agis
BACKGROUND: To understand the responses of the dental pulp to hypoxia is of high relevance for regenerative endodontics and dental traumatology. Here, we aimed to reveal the effects of hypoxia and the hypoxia mimetic agent L-mimosine (L-MIM) on the production of sclerostin (SOST) and dickkopf-1 (DKK-1) in human dental pulp-derived cells (DPC). METHODS: DPC in monolayer, spheroid and tooth slice cultures were treated with L-MIM or hypoxia. Resazurin-based toxicity and MTT assays were performed to determine cell viability...
March 9, 2018: BMC Oral Health
Xiaojia Li, Xiutian Guo, Weiqi Jin, Jingen Lu
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) and acupuncture are known to mitigate tissue damage. This study aimed to investigate the therapeutic effects of combined electroacupuncture (EA) stimulation and BMSC injection in a rat model of anal sphincter injury-induced faecal incontinence (FI). METHODS: 60 Sprague-Dawley rats were randomly divided into five groups: sham-operated control, FI, FI+EA, FI+BMSC, and FI+BMSC+EA. The anorectal tissues were collected on days 1, 3, 7 and 14...
March 8, 2018: Acupuncture in Medicine: Journal of the British Medical Acupuncture Society
Ruizeng Dong, Jianmin Guo, Zewei Zhang, Yimin Zhou, Yonghong Hua
The aim of this study was to identify the anti-cancer mechanism of Polyphyllin I (PPI) on gastric cancer cells via its activity on cancer-associated fibroblasts (CAFs). We cultured purified gastric CAFs obtained from fresh human gastric cancer tissue and examined the effect of Polyphyllin I on CAF proliferation using a colorimetric viability assay. In addition, we established a nude mouse xenograft model to examine the effect of Polyphyllin I administration on tumorigenesis. Using Western analysis, we quantified protein expression of the CAF-derived cytokines fibroblast activation protein alpha (FAP), secreted protein acidic and cysteine rich (SPARC), stromal cell-derived factor 1 (SDF-1), hepatocyte growth factor tenascin-C (TNC), and hepatocyte growth factor (HGF) in both in vitro and in vivo models...
February 27, 2018: Biochemical and Biophysical Research Communications
Rujun Zhai, Yongchun Wang, Le Qi, George Melville Williams, Bin Gao, Guang Song, James F Burdick, Zhaoli Sun
Rapid regeneration of the remnant liver is critical for preventing liver failure and promoting recovery after extensive liver resection. Numerous studies have demonstrated the involvement of bone marrow-derived stem cells in liver regeneration and the potential benefits of bone marrow stem cell therapy. To avoid the preparation of stem cells, we proposed in this study to mobilize endogenous bone marrow stem cells pharmacologically with a combination of AMD3100 (A), an antagonist of CXCR4 and low-dose FK506 (F)...
February 26, 2018: Scientific Reports
Xiangxiao Li, Weihong Sun, Wenda Xi, Weili Shen, Tong Wei, Wendong Chen, Pingjin Gao, Qun Li
Skin mesenchymal stem cells (S-MSCs) revealed an important immunomodulatory activity to markedly suppress the formation of the atherosclerosis (AS) plaque by modulating macrophages, and also inhibit the development of experimental autoimmune encephalomyelitis (EAE) by regulating T helper 17 (Th17) cell differentiation. Macrophages and Th17 cells play important roles in hypertension. However, it remains unclear whether S-MSCs are capable of improving angiotensin (AngII)-induced hypertension by acting on inflammatory cells...
February 23, 2018: Biochemical and Biophysical Research Communications
Tayfun Kara, İsmail Akaltun, Bedia Cakmakoglu, İlyas Kaya, Salih Zoroğlu
Objective: Autism spectrum disorders (ASD) have a complex pathophysiology including genetic, inflammatory and neurodevelopmental components. We aim to investigate the relationship between ASD and gene polymorphisms of stromal cell-derived factor-1 (SDF-1) and its receptor CXC chemokine receptor-4 (CXCR4), which may affect inflammatory and neurodevelopmental processes. Methods: 101 children diagnosed with ASD aged 2-18 and their biological parents were included in the study...
February 22, 2018: Psychiatry Investigation
Andrew B Goldstone, Cassandra E Burnett, Jeffery E Cohen, Michael J Paulsen, Anahita Eskandari, Bryan E Edwards, Arnar B Ingason, Amanda N Steele, Jay B Patel, John W MacArthur, Judith A Shizuru, Y Joseph Woo
Stromal cell-derived factor 1-alpha (SDF) is a potent bone marrow chemokine capable of recruiting circulating progenitor populations to injured tissue. SDF has known angiogenic capabilities, but bone marrow-derived cellular contributions to tissue regeneration remain controversial. Bone marrow from DsRed-transgenic donors was transplanted into recipients to lineage-trace circulating cells after myocardial infarction (MI). SDF was delivered post-MI, and hearts were evaluated for recruitment and plasticity of bone marrow-derived populations...
February 21, 2018: Journal of Cardiovascular Translational Research
Ru Yang, Yanhua Yao, Panjun Wang
Background: Rheumatoid arthritis (RA) is an auto-immune disease characterized by chronic inflammation of multiple joints. Hypoxia is a constant feature of synovial microenvironment in RA. Fibroblast-like synoviocytes (FLSs), which are potent effector cells in RA. It has been reported that large numbers of monocytes are recruited to the synovium and play an important role in synovial inflammation and tissue destruction in RA. However, the mechanism is still unclear. The aim of this study is to explore the role of hypoxia microenvironment on the recruitment of monocytes and then promote the development of RA...
2018: Cell & Bioscience
Qi Feng, Peng Guo, Jin Wang, Xiaoyu Zhang, Hui-Chai Yang, Jian-Gang Feng
Stromal cell-derived factor-1 (SDF-1) predicts poor clinical outcomes of certain types of cancer. Furthermore, vascular endothelial growth factor (VEGF) promotes the growth and metastasis of solid tumors. The aim of the present study was to examine the expression of SDF-1 and VEGF in patients with synovial sarcoma and to determine their expression is correlated with unfavorable outcomes. Levels of SDF-1 and VEGF proteins were evaluated in 54 patients with synovial sarcoma using immunohistochemical and immunofluorescence staining...
March 2018: Experimental and Therapeutic Medicine
Ali Asghar Peyvandi, Navid Ahmady Roozbahany, Hassan Peyvandi, Hojjat-Allah Abbaszadeh, Niloofar Majdinasab, Mohammad Faridan, Somayeh Niknazar
Previous animal studies have shown that stromal cell-derived factor-1 (SDF-1)/CXC chemokine receptor-4 (CXCR4) signaling pathway plays an important role in the targeted migration of bone marrow-derived mesenchymal stem cells (BMSCs) to the injured area. In the present study, we aimed to investigate the potential role of chemotactic SDF-1/CXCR4 signaling pathway in the homing of transplanted BMSCs to the injured cochlea after noise-induced hearing loss (NIHL) in a rat model. White noise exposure (110 dB) paradigm was used for hearing loss induction in male rats for 6 hours in 5 days...
January 2018: Neural Regeneration Research
Lizhen Liu, Qin Yu, Shan Fu, Binsheng Wang, Kaimin Hu, Limengmeng Wang, Yongxian Hu, Yulin Xu, Xiaohong Yu, He Huang
Mobilization of mesenchymal stem cells (MSCs) is an attractive strategy for cell therapy. Our previous study demonstrated that MSCs can be mobilized in circulating blood by short-term hypoxia and hypoxia-inducible factor-1α (HIF-1α) is essential for MSC mobilization. In the present study, the effect of the hypoxia mimicking agent CoCl2 was examined on MSC mobilization. The results indicated that the frequency of circulating MSCs increased slightly by administration of CoCl2. However, the mobilization efficiency was low...
February 13, 2018: Stem Cells and Development
Boopathi Sowndhar Rajan, Senthamizharasi Manivasagam, Suresh Dhanusu, Navvi Chandrasekar, Kalaiselvi Krishna, Lakshmi Priya Kalaiarasu, Aadhil Ashwaq Babu, Elangovan Vellaichamy
The present study was aimed to investigate the effect of diet derived AGEs (dAGEs) on the circulatory levels of pro-inflammatory cytokines, chemokines and to evaluate the protective efficacy of natural anti-oxidants curcumin (CU) and gallic acid (GA) respectively against the dAGEs-induced systemic inflammation in experimental Swiss albino mice. The experimental mice were fed with dAGEs in the presence and absence of CU and GA for 6 months. The levels of 40 circulatory pro-inflammatory cytokines and chemokines were evaluated using Proteome-Cytokine Array kit...
February 9, 2018: Food and Chemical Toxicology
Toshiki Kato, Vuong Cat Khanh, Kazutoshi Sato, Kosuke Takeuchi, Erica Carolina, Toshiharu Yamashita, Hisashi Sugaya, Tomokazu Yoshioka, Hajime Mishima, Osamu Ohneda
No abstract text is available yet for this article.
February 8, 2018: Biochemical and Biophysical Research Communications
W Wang, Y Xu, W J Jiao, S Zeng, B H Fan, H Yin, X P Hu, X D Zhang
Objective: To investigate the role and mechanism of IL-17 and CXCR4 in chronic graft nephropathy (CAN) in rat models. Methods: CAN rat models were established using Fisher 344 to Lewis rats. In the control group( n =10), Lewis rats were performed isotransplantation. CAN rat models were established in experimental group( n =10). All the rats were havested 6 weeks after transplantation. Kidneys were examined by pathology to evaluate the injury of the renal allograft. SDF-1/CXCR4, IL-17 and α-SMA expression level in serum and renal graft were detected by immunohistochemical staining and Enzyme-linked immunosorbent assays(ELISA)...
January 30, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Zaixing Yang, Yan Liang, Feng Lin, Zhencheng Zhang, Wanwan Luo, Yuting Zhang, Renqian Zhong
BACKGROUND: Stromal cell-derived factor-1 (SDF-1), also called chemokine (C-X-C motif) ligand 12 (CXCL12), as a chemokine for premature B cells, T cells and monocytes, is detected in liver, pancreas, spleen and heart. However, its diagnostic value for primary biliary cholangitis (PBC) as well as the association of SDF-1 with inflammatory and fibrotic progression is unclear. The aim of this study was to determine serum stromal cell-derived factor-1 (SDF-1) level and to explore its diagnostic value for primary biliary cholangitis (PBC) as well as the association of SDF-1 with inflammatory and fibrotic progression of PBC...
February 2, 2018: International Immunopharmacology
Caiyun Shan, Yongsheng Ma
Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways using a stroke model in vivo and a cell migration model in vitro. Bone marrow‑derived endothelial progenitor cells (EPCs) were extracted and identified using a density gradient method. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was performed to examine the expression levels of miR‑126 and C‑X‑C chemokine receptor type 7 (CXCR7)...
January 29, 2018: Molecular Medicine Reports
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