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https://www.readbyqxmd.com/read/29784328/determination-of-endrin-and-%C3%AE-keto-endrin-in-five-food-products-of-animal-origin-using-gc-%C3%AE-ecd-a-modified-quechers-approach-to-traditional-detection
#1
Md Musfiqur Rahman, Han Sol Lee, A M Abd El-Aty, Md Humayun Kabir, Hyung Suk Chung, Jong-Hyouk Park, Mi-Ra Kim, Ji-Hyun Kim, Ho-Chul Shin, Sung Shik Shin, Jae-Han Shim
A simple quick, easy, cheap, effective, rugged, and safe (QuEChERS)-based method was developed for the analysis of endrin and its metabolite, δ-keto endrin, in five animal-derived food products (chicken, pork, beef, egg, and milk) using a gas chromatography-micro electron capture detector (GC-μECD). Samples were extracted with acidified acetonitrile, salted out with magnesium sulfate and sodium acetate, and finally purified with a dual layer solid-phase extraction cartridge (SPE) that contains both Supelclean ENVI-Carb (upper layer) and primary secondary amine (lower layer) SPE sorbents...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29775894/positive-effects-of-antitumor-drugs-in-combination-with-propolis-on-canine-osteosarcoma-cells-spos-2-and-mesenchymal-stem-cells
#2
Pedro Negri Bernardino, Paulo Ricardo Oliveira Bersano, João Ferreira Lima Neto, José Maurício Sforcin
The combination of lower concentrations of antitumor drugs (carboplatin - CARB, doxorubicin - DOX, and methotrexate - MET) with propolis was investigated against canine osteosarcoma (spOS-2) and mesenchymal stem cells (MSC) in vitro. The mechanism of action in the combinations was analyzed. spOS-2 cells were incubated up to 72 h with propolis (50 μg/ml) alone or in combination with CARB (10-400 μmol/l), DOX (0.5-2 μmol/l) or MET (50-200 μmol/l). Cell viability was assessed by MTT assay, apoptosis/necrosis by flow cytometry, and MSC was incubated with the optimum combination...
May 15, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29765767/crystal-water-as-the-mol-ecular-glue-for-obtaining-different-co-crystal-ratios-the-case-of-gallic-acid-tris-caffeine-hexa-hydrate
#3
L Vella-Zarb, U Baisch
The crystal structure of the hexa-hydrate co-crystal of gallic acid and caffeine, C7 H6 O5 ·3C8 H10 N4 O2 ·6H2 O or GAL3CAF·6H2 O , is a remarkable example of the importance of hydrate water acting as structural glue to facilitate the crystallization of two components of different stoichiometries and thus to compensate an imbalance of hydrogen-bond donors and acceptors. The water mol-ecules provide the additional hydrogen bonds required to form a crystalline solid. Whereas the majority of hydrogen bonds forming the inter-molecular network between gallic acid and caffeine are formed by crystal water, only one direct classical hydrogen bond between two mol-ecules is formed between the carb-oxy-lic oxygen of gallic acid and the carbonyl oxygen of caffeine with d ( D ⋯ A ) = 2...
April 1, 2018: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/29765728/different-mol-ecular-conformations-in-the-crystal-structures-of-three-5-nitro-imidazolyl-derivatives
#4
Luis F B Osorio, Samir A Carvalho, Edson F da Silva, Carlos A M Fraga, Solange M S V Wardell, Bruce F Milne, James L Wardell, William T A Harrison
The crystal structures of ( E )-1-methyl-5-nitro-1 H -imidazole-2-carbaldehyde O -benzyl-oxime, C12 H12 N4 O3 , (I), ( E )-1-methyl-5-nitro-1 H -imidazole-2-carb-alde-hyde O -(4-fluoro-benz-yl) oxime, C12 H11 FN4 O3 , (II), and ( E )-1-methyl-5-nitro-1 H -imidazole-2-carbaldehyde O -(4-bromo-benz-yl) oxime, C12 H11 BrN4 O3 , (III), are described. The dihedral angle between the ring systems in (I) is 49.66 (5)° and the linking Nm -C-C=N (m = methyl-ated) bond shows an anti conformation [torsion angle = 175...
March 1, 2018: Acta Crystallographica. Section E, Crystallographic Communications
https://www.readbyqxmd.com/read/29753682/class-a-%C3%AE-lactamases-and-inhibitors-in-silico-analysis-of-the-binding-mode-and-the-relationship-with-resistance
#5
Rebeca Pereira, Vitor Won-Held Rabelo, Alexander Sibajev, Paula Alvarez Abreu, Helena Carla Castro
β-lactams are one of the most common antimicrobials used to treat bacterial infections. However, bacterial resistance has compromised their efficacy, mainly due to β-lactamase enzyme production. To overcome this resistance, β-lactamase inhibitors can be used in association with these antimicrobials. Herein, we analyzed the structural characteristics of β-lactamases and their interactions with classical inhibitors, such as clavulanic acid (CA), sulbactam (SB) and tazobactam (TZ) to gain insights into resistance...
May 10, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29735572/how-low-can-you-go-does-lower-carb-translate-to-lower-glucose
#6
Carly Runge, Joyce M Lee
No abstract text is available yet for this article.
May 7, 2018: Pediatrics
https://www.readbyqxmd.com/read/29655724/solid-phase-extraction-with-high-polarity-carb-psa-as-composite-fillers-prior-to-uplc-ms-ms-to-determine-six-bisphenols-and-alkylphenols-in-trace-level-hotpot-seasoning
#7
Hao Dong, Xiaofang Zeng, Weidong Bai
The present study reports an ultra high-performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of six bisphenols (bisphenol A, bisphenol B and bisphenol F) and alkylphenols (4-nonylphenol, 4-n-nonylphenol and octylphenol) in hotpot seasoning. Samples were dispersed in n-hexane after addition of internal standards bisphenol A-d4 and 4-n-nonylphenol-d4 . Sample solutions were then centrifuged, and the supernatants purified using solid phase extraction with high polarity Carb/PSA composite fillers...
August 30, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29652244/deriving-fuel-based-emission-factor-thresholds-to-interpret-heavy-duty-vehicle-roadside-plume-measurements
#8
David C Quiros, Jeremy D Smith, Walter A Ham, William H Robertson, Tao Huai, Alberto Ayala, Shaohua Hu
Remote sensing devices have been used for decades to measure gaseous emissions from individual vehicles at the roadside. Systems have also been developed that entrain diluted exhaust and can also measure particulate matter (PM) emissions. In 2015, the California Air Resources Board (CARB) reported that 8% of in-field diesel particulate filters (DPF) on heavy-duty (HD) vehicles were malfunctioning and emitted about 70% of total diesel PM emissions from the DPF-equipped fleet. A new high-emitter problem in the heavy-duty vehicle fleet had emerged...
April 13, 2018: Journal of the Air & Waste Management Association
https://www.readbyqxmd.com/read/29626108/carb-loading-takes-proteins-on-a-ride
#9
Christina Schäffer, Paul Messner
Cytoplasmic protein O -glycosylation in bacteria is often required for protein maturation, but the dependence of protein export on carbohydrate modifications is less understood. In the current issue of JBC , Chen et al. describe the mechanism for posttranslational modification of a Streptococcus gordonii adhesin and its delivery to the membrane, leading to the first comprehensive model featuring the interplay of glycosyltransferases and the translocation system.
April 6, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29604174/the-use-of-an-injection-port-is-helpful-in-improving-metabolic-control-and-compliance-to-a-strict-basal-bolus-regimen-in-children-and-adolescents-with-type-1-diabetes
#10
Giulio Maltoni, Maximiliano Zioutas, Stefano Zucchini, Andrea Pession
Basal-bolus regimen is the best treatment for children with type 1 diabetes (T1D), but requires several insulin injections a day1 . In fact, the body's physical growth, the management of frequent snacks and mistakes in carb-counting and in insulin boluses require the ability to promptly adapt insulin scheme2,3 .
March 31, 2018: Journal of Diabetes
https://www.readbyqxmd.com/read/29541907/overweight-and-diabetes-prevention-is-a-low-carbohydrate-high-fat-diet-recommendable
#11
REVIEW
Fred Brouns
In the past, different types of diet with a generally low-carbohydrate content (< 50-< 20 g/day) have been promoted, for weight loss and diabetes, and the effectiveness of a very low dietary carbohydrate content has always been a matter of debate. A significant reduction in the amount of carbohydrates in the diet is usually accompanied by an increase in the amount of fat and to a lesser extent, also protein. Accordingly, using the term "low carb-high fat" (LCHF) diet is most appropriate...
June 2018: European Journal of Nutrition
https://www.readbyqxmd.com/read/29535083/low-calorie-and-low-carb-diets-for-weight-loss-in-primary-care
#12
Jane Feinmann
No abstract text is available yet for this article.
March 13, 2018: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/29514363/comparison-of-techniques-for-preimplantation-treatment-of-osteochondral-allograft-bone
#13
Charles A Baumann, John R Baumann, Chantelle C Bozynski, Aaron M Stoker, James P Stannard, James L Cook
Articular defects are a major problem with few effective treatment options. Osteochondral allograft (OCA) transplantation can be an effective treatment; however, lack of OCA bone integration can cause failure. This controlled laboratory study was designed to compare clinically applicable methods for marrow element removal and enhanced delivery of bone marrow aspirate concentrate (BMC) to OCA bone. We hypothesized that compressed carbon dioxide (CO2 ) treatment of OCA bone would result in significantly better marrow element removal, significantly more retention and distribution of viable osteoprogenitor cells, and significantly higher osteoinductive protein elution from OCAs compared with other preimplantation treatments...
March 7, 2018: Journal of Knee Surgery
https://www.readbyqxmd.com/read/29452189/roasted-and-green-coffee-extracts-show-antioxidant-and-cytotoxic-activity-in-myoblast-and-endothelial-cell-lines-in-a-cell-specific-manner
#14
Alexandros Priftis, Eleftheria-Maria Panagiotou, Konstantinos Lakis, Christina Plika, Maria Halabalaki, Georgia Ntasi, Aristidis S Veskoukis, Dimitrios Stagos, Leandros A Skaltsounis, Dimitrios Kouretas
Coffee is one of the most highly consumed beverages with potential beneficial health implications, however its molecular mechanism of action has not been completely elucidated yet. To that cause, the polyphenolic composition of different coffee extracts (from Light, Medium and Dark roasts as well as green beans) was examined by UHPLC-HRMS analysis, indicating chlorogenic acids isomers as the main constituents. In the following step, the toxicity of the extracts was tested in myoblasts and endothelial cells and differential toxicity of green and roasted samples was displayed as the myoblasts were more sensitive to green coffee extracts, in contrast to the endothelial cells...
April 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/29446894/the-low-carb-craze
#15
(no author information available yet)
No abstract text is available yet for this article.
May 2017: Diabetes Forecast
https://www.readbyqxmd.com/read/29385034/liver-fat-scores-moderately-reflect-interventional-changes-in-liver-fat-content-by-a-low-fat-diet-but-not-by-a-low-carb-diet
#16
Stefan Kabisch, Sabrina Bäther, Ulrike Dambeck, Margrit Kemper, Christiana Gerbracht, Caroline Honsek, Anna Sachno, Andreas F H Pfeiffer
BACKGROUND: Non-alcoholic fatty liver disease (NAFLD) is a common metabolic disorder all over the world, mainly being associated with a sedentary lifestyle, adiposity, and nutrient imbalance. The increasing prevalence of NAFLD accommodates similar developments for type 2 diabetes and diabetes-related comorbidities and complications. Therefore, early detection of NAFLD is an utmost necessity. Potentially helpful tools for the prediction of NAFLD are liver fat indices. The fatty liver index (FLI) and the NAFLD-liver fat score (NAFLD-LFS) have been recently introduced for this aim...
January 31, 2018: Nutrients
https://www.readbyqxmd.com/read/29371556/carotenoid-biosynthesis-in-fusarium
#17
REVIEW
Javier Avalos, Javier Pardo-Medina, Obdulia Parra-Rivero, Macarena Ruger-Herreros, Roberto Rodríguez-Ortiz, Dámaso Hornero-Méndez, María Carmen Limón
Many fungi of the genus Fusarium stand out for the complexity of their secondary metabolism. Individual species may differ in their metabolic capacities, but they usually share the ability to synthesize carotenoids, a family of hydrophobic terpenoid pigments widely distributed in nature. Early studies on carotenoid biosynthesis in Fusarium aquaeductuum have been recently extended in Fusarium fujikuroi and Fusarium oxysporum , well-known biotechnological and phytopathogenic models, respectively. The major Fusarium carotenoid is neurosporaxanthin, a carboxylic xanthophyll synthesized from geranylgeranyl pyrophosphate through the activity of four enzymes, encoded by the genes carRA , carB , carT and carD ...
July 7, 2017: Journal of Fungi (Basel, Switzerland)
https://www.readbyqxmd.com/read/29368448/low-carbohydrate-diets-may-increase-risk-of-neural-tube-defects
#18
Tania A Desrosiers, Anna Maria Siega-Riz, Bridget S Mosley, Robert E Meyer
BACKGROUND: Folic acid fortification significantly reduced the prevalence of neural tube defects (NTDs) in the United States. The popularity of "low carb" diets raises concern that women who intentionally avoid carbohydrates, thereby consuming fewer fortified foods, may not have adequate dietary intake of folic acid. METHODS: To assess the association between carbohydrate intake and NTDs, we analyzed data from the National Birth Defects Prevention Study from 1,740 mothers of infants, stillbirths, and terminations with anencephaly or spina bifida (cases), and 9,545 mothers of live born infants without a birth defect (controls) conceived between 1998 and 2011...
January 25, 2018: Birth Defects Research
https://www.readbyqxmd.com/read/29351387/letter-to-the-editor-low-carb-meal-plan-and-postmeal-exercise
#19
Elsamma Chacko
No abstract text is available yet for this article.
December 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29344811/metabolic-evolution-and-a-comparative-omics-analysis-of-corynebacterium-glutamicum-for-putrescine-production
#20
Zhen Li, Yu-Ping Shen, Xuan-Long Jiang, Li-Shen Feng, Jian-Zhong Liu
Putrescine is widely used in the industrial production of bioplastics, pharmaceuticals, agrochemicals, and surfactants. Because the highest titer of putrescine is much lower than that of its precursor L-ornithine reported in microorganisms to date, further work is needed to increase putrescine production in Corynebacterium glutamicum. We first compared 7 ornithine decarboxylase genes and found that the Enterobacter cloacae ornithine decarboxylase gene speC1 was most suitable for putrescine production in C. glutamicum...
January 17, 2018: Journal of Industrial Microbiology & Biotechnology
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