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R Kalluri

Jaya Arya, Sebastien Henry, Haripriya Kalluri, Devin V McAllister, Winston P Pewin, Mark R Prausnitz
To support translation of microneedle patches from pre-clinical development into clinical trials, this study examined the effect of microneedle patch application on local skin reactions, reliability of use and acceptability to patients. Placebo patches containing dissolving microneedles were administered to fifteen human participants. Microneedle patches were well tolerated in the skin with no pain or swelling and only mild erythema localized to the site of patch administration that resolved fully within seven days...
June 2017: Biomaterials
Sujuan Yang, Sara P Y Che, Paul Kurywchak, Jena L Tavormina, Liv B Gansmo, Pedro Correa de Sampaio, Michael Tachezy, Maximilian Bockhorn, Florian Gebauer, Amanda R Haltom, Sonia A Melo, Valerie S LeBleu, Raghu Kalluri
Pancreatic cancer presents with a dismal mortality rate and is in urgent need of methods for early detection with potential for timely intervention. All living cells, including cancer cells, generate exosomes. We previously discovered double stranded genomic DNA in exosomes derived from the circulation of pancreatic cancer patients, which enabled the detection of prevalent mutations associated with the disease. Here, we report a proof-of-concept study that demonstrates the potential clinical utility of circulating exosomal DNA for identification of KRAS(G)(12)(D) and TP53(R)(273)(H) mutations in patients with pancreas-associated pathologies, including pancreatic ductal adenocarcinoma (PDAC), chronic pancreatitis (CP) and intraductal papillary mucinous neoplasm (IPMN), and in healthy human subjects...
March 4, 2017: Cancer Biology & Therapy
Jhansi R Kalluri, Roberto Gonzalez-Rodriguez, Phil S Hartman, Armando Loni, Leigh T Canham, Jeffery L Coffer
Multiple new approaches to tackle multidrug resistant infections are urgently needed and under evaluation. One nanotechnology-based approach to delivering new relevant therapeutics involves silicon accumulator plants serving as a viable silicon source in green routes for the fabrication of the nanoscale drug delivery carrier porous silicon (pSi). If the selected plant leaf components contain medicinally-active species as well, then a single substance can provide not only the nanoscale high surface area drug delivery carrier, but the drug itself...
2016: PloS One
Timothy P Cleland, Elena R Schroeter, Leonid Zamdborg, Wenxia Zheng, Ji Eun Lee, John C Tran, Marshall Bern, Michael B Duncan, Valerie S Lebleu, Dorothy R Ahlf, Paul M Thomas, Raghu Kalluri, Neil L Kelleher, Mary H Schweitzer
Structures similar to blood vessels in location, morphology, flexibility, and transparency have been recovered after demineralization of multiple dinosaur cortical bone fragments from multiple specimens, some of which are as old as 80 Ma. These structures were hypothesized to be either endogenous to the bone (i.e., of vascular origin) or the result of biofilm colonizing the empty osteonal network after degradation of original organic components. Here, we test the hypothesis that these structures are endogenous and thus retain proteins in common with extant archosaur blood vessels that can be detected with high-resolution mass spectrometry and confirmed by immunofluorescence...
December 4, 2015: Journal of Proteome Research
Elena V Vassilieva, Haripriya Kalluri, Devin McAllister, Misha T Taherbhai, E Stein Esser, Winston P Pewin, Joanna A Pulit-Penaloza, Mark R Prausnitz, Richard W Compans, Ioanna Skountzou
Prevention of seasonal influenza epidemics and pandemics relies on widespread vaccination coverage to induce protective immunity. In addition to a good antigenic match with the circulating viruses, the effectiveness of individual strains represented in the trivalent vaccines depends on their immunogenicity. In this study, we evaluated the immunogenicity of H1N1, H3N2, and B seasonal influenza virus vaccine strains delivered individually with a novel dissolving microneedle patch and the stability of this formulation during storage at 25 °C...
August 2015: Drug Delivery and Translational Research
Nicolas Léveillé, Carlos A Melo, Koos Rooijers, Angel Díaz-Lagares, Sonia A Melo, Gozde Korkmaz, Rui Lopes, Farhad Akbari Moqadam, Ana R Maia, Patrick J Wijchers, Geert Geeven, Monique L den Boer, Raghu Kalluri, Wouter de Laat, Manel Esteller, Reuven Agami
p53 binds enhancers to regulate key target genes. Here, we globally mapped p53-regulated enhancers by looking at enhancer RNA (eRNA) production. Intriguingly, while many p53-induced enhancers contained p53-binding sites, most did not. As long non-coding RNAs (lncRNAs) are prominent regulators of chromatin dynamics, we hypothesized that p53-induced lncRNAs contribute to the activation of enhancers by p53. Among p53-induced lncRNAs, we identified LED and demonstrate that its suppression attenuates p53 function...
March 27, 2015: Nature Communications
Dimitrios G Koutsonanos, E Stein Esser, Sean R McMaster, Priya Kalluri, Jeong-Woo Lee, Mark R Prausnitz, Ioanna Skountzou, Timothy L Denning, Jacob E Kohlmeier, Richard W Compans
Skin has gained substantial attention as a vaccine target organ due to its immunological properties, which include a high density of professional antigen presenting cells (APCs). Previous studies have demonstrated the effectiveness of this vaccination route not only in animal models but also in adults. Young children represent a population group that is at high risk from influenza infection. As a result, this group could benefit significantly from influenza vaccine delivery approaches through the skin and the improved immune response it can induce...
September 8, 2015: Vaccine
Berna C Özdemir, Tsvetelina Pentcheva-Hoang, Julienne L Carstens, Xiaofeng Zheng, Chia-Chin Wu, Tyler R Simpson, Hanane Laklai, Hikaru Sugimoto, Christoph Kahlert, Sergey V Novitskiy, Ana De Jesus-Acosta, Padmanee Sharma, Pedram Heidari, Umar Mahmood, Lynda Chin, Harold L Moses, Valerie M Weaver, Anirban Maitra, James P Allison, Valerie S LeBleu, Raghu Kalluri
Pancreatic ductal adenocarcinoma (PDAC) is associated with marked fibrosis and stromal myofibroblasts, but their functional contribution remains unknown. Transgenic mice with the ability to delete αSMA(+) myofibroblasts in pancreatic cancer were generated. Depletion starting at either noninvasive precursor (pancreatic intraepithelial neoplasia) or the PDAC stage led to invasive, undifferentiated tumors with enhanced hypoxia, epithelial-to-mesenchymal transition, and cancer stem cells, with diminished animal survival...
June 16, 2014: Cancer Cell
Ervin R Hafter, Jing Xia, Sridhar Kalluri
While studies of simple acoustic features have provided excellent bases for models of spatial hearing, we are seeking, here, to create a new paradigm for examination of shared attention and scene analysis in natural environments, where the listener is confronted with semantic information from multiple sources. In this new simulation of the cocktail party problem, a subject (S) is questioned, on-line, about information heard in multiple simultaneous stories spoken by different talkers. Questions based on brief passages in the stories are presented visually for manual response...
2013: Advances in Experimental Medicine and Biology
Michael B Duncan, Changqing Yang, Harikrishna Tanjore, Patrick M Boyle, Doruk Keskin, Hikaru Sugimoto, Michael Zeisberg, Bjorn R Olsen, Raghu Kalluri
The regenerative response to drug- and toxin-induced liver injury induces changes to the hepatic stroma, including the extracellular matrix. Although the extracellular matrix is known to undergo changes during the injury response, its impact on maintaining hepatocyte function and viability in this process remains largely unknown. We demonstrate that recovery from toxin-mediated injury is impaired in mice deficient in a key liver extracellular matrix molecule, type XVIII collagen, and results in rapid death...
July 2013: Disease Models & Mechanisms
Asha Anish Madhavan, Gopika Gopa Kumar, Sujith Kalluri, John Joseph, Sivakumar Nagarajan, Shantikumar Nair, Kavasseri R V Subramanian, Avinash Balakrishnan
The present study demonstrates an original approach by which Au nanoparticles (approximately 10 nm) are embedded into TiO2 fibers via electrospinning. The photocatalytic performance of the resultant fibrous material was studied and related to the architecture and the nature of the internal interfaces in the composite. It was found that embedment of nano Au particles into the TiO2 fiber significantly improved the photocatalytic performance as compared to non-embedded ones. Electrospun fibers with the Au nanoparticles (approximately 10 nm) showed an average fiber diameter of approximately 380 nm...
October 2012: Journal of Nanoscience and Nanotechnology
R K Kalluri, M M Biener, M E Suss, M D Merrill, M Stadermann, J G Santiago, T F Baumann, J Biener, A Striolo
Understanding and leveraging physicochemical processes at the pore scale are believed to be essential to future performance improvements of supercapacitors and capacitive desalination (CD) cells. Here, we report on a combination of electrochemical experiments and fully atomistic simulations to study the effect of pore size and surface charge density on the capacitance of graphitic nanoporous carbon electrodes. Specifically, we used cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) to study the effect of potential and pore size on the capacitance of nanoporous carbon foams...
February 21, 2013: Physical Chemistry Chemical Physics: PCCP
M Banati, P Csecsei, E Koszegi, H H Nielsen, G Suto, L Bors, A Trauninger, T Csepany, C Rozsa, G Jakab, T Molnar, A Berthele, S R Kalluri, T Berki, Z Illes
BACKGROUND: Antibodies against gastrointestinal antigens may indicate altered microbiota and immune responses in the gut. Recent experimental data suggest a connection between gastrointestinal immune responses and CNS autoimmunity. METHODS: Antibodies against gliadin, tissue transglutaminase (tTG), intrinsic factor (IF), parietal cells (PC) and Saccharomyces cerevisiae (ASCA) were screened in the sera of 45 patients with AQP4-seropositive neuromyelitis optica (NMO) and NMO spectrum diseases (NMO/NMO-SD), 17 patients with AQP4-seronegative NMO, 85 patients with clinically definite multiple sclerosis (MS), and 48 healthy controls (HC)...
November 2013: European Journal of Neurology: the Official Journal of the European Federation of Neurological Societies
Asha Anish Madhavan, Sujith Kalluri, Anjali Paravannoor, Sivakumar Nagarajan, Kavasseri R V Subramanian, Shantikumar Nair, Avinash Balakrishnan
The present study demonstrates a novel approach by which titanium foils coated with electropolymerized poly(3,4-ethylenedioxythiophene) (PEDOT) in combination with sputtered platinum can be processed into a high-surface area cathodes for dye-sensitized solar cells (DSSCs). A detailed study has been performed to elucidate how surface nanomorphology and I(-)/I(3-) redox reaction behaviors underlying these photocathodes impact the DSSC performances. From the analysis of the relevant electrochemical parameters, an intrinsic correlation between the photovoltaic performances and the cathode surface area has been deduced for such a system and explained on the basis of relative contributions of the galvanic coupling properties of the nanomorphology PEDOT film and platinum...
August 2012: Journal of Nanoscience and Nanotechnology
S Suma, G Anita, B R Chandra Shekar, Amitabh Kallury
BACKGROUND: Bonding brackets to fluorosed enamel remains a clinical challenge and bracket failure at the compromised enamel interface is common. OBJECTIVE: To check the effect of air abrasion on the retention of metallic brackets bonded to fluorosed enamel surface. MATERIALS AND METHODS: Sixty freshly extracted human premolar teeth having moderate to severe dental fluorosis as per Dean's criteria were collected and divided into three groups of 20 each...
March 2012: Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research
Don N Ho, Nathan Kohler, Aruna Sigdel, Raghu Kalluri, Jeffrey R Morgan, Chenjie Xu, Shouheng Sun
Iron oxide nanoparticles are a useful diagnostic contrast agent and have great potential for therapeutic applications. Multiple emerging diagnostic and therapeutic applications and the numerous versatile parameters of the nanoparticle platform require a robust biological model for characterization and assessment. Here we investigate the use of iron oxide nanoparticles that target tumor vasculature, via the tumstatin peptide, in a novel three-dimensional tissue culture model. The developed tissue culture model more closely mimics the in vivo environment with a leaky endothelium coating around a glioma tumor mass...
2012: Theranostics
Vesselina G Cooke, Valerie S LeBleu, Doruk Keskin, Zainab Khan, Joyce T O'Connell, Yingqi Teng, Michael B Duncan, Liang Xie, Genta Maeda, Sylvia Vong, Hikaru Sugimoto, Rafael M Rocha, Aline Damascena, Ricardo R Brentani, Raghu Kalluri
The functional role of pericytes in cancer progression remains unknown. Clinical studies suggest that low numbers of vessel-associated pericytes correlated with a drop in overall survival of patients with invasive breast cancer. Using genetic mouse models or pharmacological inhibitors, pericyte depletion suppressed tumor growth but enhanced metastasis. Pericyte depletion was further associated with increased hypoxia, epithelial-to-mesenchymal transition (EMT), and Met receptor activation. Silencing of Twist or use of a Met inhibitor suppressed hypoxia and EMT/Met-driven metastasis...
January 17, 2012: Cancer Cell
Cetin Tas, Saffar Mansoor, Haripriya Kalluri, Vladimir G Zarnitsyn, Seong-O Choi, Ajay K Banga, Mark R Prausnitz
Peptides and polypeptides have important pharmacological properties but only a limited number have been exploited as therapeutics because of problems related to their delivery. Most of these drugs require a parenteral delivery system which introduces the problems of pain, possible infection, and expertise required to carry out an injection. The aim of this study was to develop a transdermal patch containing microneedles (MNs) coated with a peptide drug, salmon calcitonin (sCT), as an alternative to traditional subcutaneous and nasal delivery routes...
February 28, 2012: International Journal of Pharmaceutics
Paul Abraham, Rachel Adams, Richard J Giannone, Udaya Kalluri, Priya Ranjan, Brian Erickson, Manesh Shah, Gerald A Tuskan, Robert L Hettich
Current state-of-the-art experimental and computational proteomic approaches were integrated to obtain a comprehensive protein profile of Populus vascular tissue. This featured: (1) a large sample set consisting of two genotypes grown under normal and tension stress conditions, (2) bioinformatics clustering to effectively handle gene duplication, and (3) an informatics approach to track and identify single amino acid polymorphisms (SAAPs). By applying a clustering algorithm to the Populus database, the number of protein entries decreased from 64,689 proteins to a total of 43,069 protein groups, thereby reducing 7505 identified proteins to a total of 4226 protein groups, in which 2016 were singletons...
January 1, 2012: Journal of Proteome Research
Joyce T O'Connell, Hikaru Sugimoto, Vesselina G Cooke, Brian A MacDonald, Ankit I Mehta, Valerie S LeBleu, Rajan Dewar, Rafael M Rocha, Ricardo R Brentani, Murray B Resnick, Eric G Neilson, Michael Zeisberg, Raghu Kalluri
Increased numbers of S100A4(+) cells are associated with poor prognosis in patients who have cancer. Although the metastatic capabilities of S100A4(+) cancer cells have been examined, the functional role of S100A4(+) stromal cells in metastasis is largely unknown. To study the contribution of S100A4(+) stromal cells in metastasis, we used transgenic mice that express viral thymidine kinase under control of the S100A4 promoter to specifically ablate S100A4(+) stromal cells. Depletion of S100A4(+) stromal cells significantly reduced metastatic colonization without affecting primary tumor growth...
September 20, 2011: Proceedings of the National Academy of Sciences of the United States of America
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