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Sperm agglutination

Elisabeth Pinart, Esther Domènech, Eva Bussalleu, Marc Yeste, Sergi Bonet
The present study compares the sperm quality of boar seminal doses artificially inoculated with Escherichia coli and Clostridium perfringens, and maintained in liquid storage at 15°C for a 9-day period. Seminal doses from 10 sexually mature Piétrain boars were diluted in a Beltsville Thawing Solution (BTS)-based extender and infected either with E. coli or C. perfringens, with bacterial loads ranging from 10(1) to 10(7)cfumL(-1). During storage, the changes in sperm quality were determined by assessing pH, sperm viability, sperm motiliy, sperm morphology, sperm agglutination degree, and sperm-bacteria interaction...
December 13, 2016: Animal Reproduction Science
S K Srivastava, S Shinde, S K Singh, S Mehrotra, M R Verma, A K Singh, S Nandi, N Srivastava, S K Singh, T K Goswami, S K Bhure, H Kumar, S K Ghosh
Antisperm antibodies have been found in repeat-breeding(RB) cows, and those causing agglutination and/or immobilization of sperm are considered to be closely related to unexplained infertility. However, a standard protocol for identifying antisperm antibodies (ASA) in cattle is not validated. Therefore, an investigation was undertaken to evaluate sperm immobilization (SIT), sperm agglutination (SAT) and immunoperoxidase (IPT)assays for detection of ASA in serum and their respective threshold levels for confirmation...
December 8, 2016: Reproduction in Domestic Animals, Zuchthygiene
D Gączarzewicz, J Udała, M Piasecka, B Błaszczyk, T Stankiewicz
This study was designed to determine the degree and type of bacterial contamination in boar semen (79 ejaculates from Large White and Landrace boars) and its consequences for sperm quality during storage (27 extended semen samples, 16°C for five days) under practical conditions of artificial insemination (AI). The results revealed the presence of aerobic bacteria in 99% of the ejaculates (from 80 to 370 ×106 colony-forming units/mL). Most of the ejaculates contained two or three bacterial contaminants, while the Staphylococcus, Streptococcus, and Pseudomonas bacterial genera were most frequently isolated...
September 1, 2016: Polish Journal of Veterinary Sciences
Gergely Bernáth, Daniel Żarski, Eszter Kása, Ádám Staszny, Levente Várkonyi, Tímea Kollár, Árpád Hegyi, Zoltán Bokor, Béla Urbányi, Ákos Horváth
The applicability of a programmable freezer for the increased-scale cryopreservation of common carp sperm was investigated. The effect of different equilibration times, cryopreservation methods, extenders, dilution ratios, activating solutions on the post-thaw motility of common carp sperm was investigated. The suitable post-thaw storage time-interval as well as fertilizing capacity of cryopreserved sperm was also examined. The motility, curvilinear velocity (VCL) and straightness (STR) values did not decrease significantly during 60min of equilibration neither in equilibrated nor thawed groups...
October 1, 2016: General and Comparative Endocrinology
Joaquín Cordero-Martínez, Charmina Aguirre-Alvarado, Jessica Gabriela Guzmán-Soriano, Cinthia Erika Sánchez-Arroyo, Juan Carlos Flores-Alonso, Lorena Rodríguez-Páez
UNLABELLED: The present study evaluates the possible antifertility effect of aqueous crude extract (OBACE) of Echeveria gibbiflora, a plant that belongs to the crassulaceae family, used in traditional Mexican medicine as a vaginal post coital rinse to prevent pregnancy and shown to have an immobilization/agglutination effect on sperm of different mammal species. We evaluated the effect of OBACE on functional parameters of mouse sperm, such as viability, capacitation, and acrosome reaction...
October 2016: Systems Biology in Reproductive Medicine
Marta F Riesco, Francisca Félix, Domitilia Matias, Sandra Joaquim, Marc Suquet, Elsa Cabrita
Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry...
May 7, 2016: General and Comparative Endocrinology
Davide Monaco, Meriem Fatnassi, Barbara Padalino, Mohamed Hammadi, Touhami Khorchani, Giovanni Michele Lacalandra
Ejaculates from five clinically healthy dromedary camels (Camelus dromedarius) were used to evaluate the effects of different enzymatic treatments (Amylase, Papain, Spermfluid) on liquefaction and seminal parameters. After collection, ejaculates were divided into 5 aliquots: (1) kept undiluted (control); or diluted 1:1 with: (2) Tris-Citrate-Fructose (TCF), (3) TCF containing Amylase, (4) TCF containing Papain or (5) Spermfluid containing Bromelain. At 120 min after dilution, each aliquot was evaluated, at 20-min intervals, for viscosity, motility, viability and agglutination...
April 2016: Research in Veterinary Science
Maria Angeles Monclus, Miguel Walter Fornes
In many mammalian and non-mammalian species, mature sperm interact within the female reproductive tract or inside the epididymal lumen using cohesive forces. This phenomenon, known as "sperm conjugation", is sometimes confused with sperm agglutination, which is the result of the interaction of epididymal or ejaculate spermatozoa upon release into culture medium. In addition to "agglutination", the terms "association", "rouleaux", or "rosettes" are employed interchangeably to describe the conjugation phenomenon, which inevitably causes confusion due to the non-unifying nomenclature...
March 11, 2016: Molecular Reproduction and Development
Jun Fu, Rongyan Yao, Yanyun Luo, Dantong Yang, Yang Cao, Yi Qiu, Wei Song, Shiying Miao, Yiqun Gu, Linfang Wang
Infertility is currently a major public health problem. Anti-sperm antibodies (ASAs) markedly reduce sperm quality, which can subsequently lead to male and/or female infertility. The accurate detection of ASAs derived from specific spermatozoa is, therefore, clinically useful. We have focused on the spermatozoa-specific expression protein ACTL7a for many years and have developed an enzyme-linked immunosorbent assay (ELISA) to detect the concentration of anti-ACTL7a antibodies in fertile sera (n = 267) and infertile sera (n = 193)...
March 9, 2016: Scientific Reports
T Leahy, J P Rickard, R J Aitken, S P de Graaf
Head-to-head agglutination of ram spermatozoa is induced by dilution in the Tyrode's capacitation medium with albumin, lactate and pyruvate (TALP) and ameliorated by the addition of the thiol d-penicillamine (PEN). To better understand the association and disassociation of ram spermatozoa, we investigated the mechanism of action of PEN in perturbing sperm agglutination. PEN acts as a chelator of heavy metals, an antioxidant and a reducing agent. Chelation is not the main mechanism of action, as the broad-spectrum chelator ethylenediaminetetraacetic acid and the copper-specific chelator bathocuproinedisulfonic acid were inferior anti-agglutination agents compared with PEN...
May 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
Jun Fu, Rongyan Yao, Yanyun Luo, Dantong Yang, Yang Cao, Yi Qiu, Wei Song, Shiying Miao, Yiqun Gu, Linfang Wang
Numerous investigations have focused on the detection of antisperm antibodies, which have a naturally occurring impact on male and female fertility. In this study, spermatogenic glyceraldehyde-3-phosphate dehydrogenase (GAPDHS) was considered to be a candidate biomarker of immune infertility. The concentrations of anti-GAPDHS antibodies in the sera of sterile individuals and fertile couples were measured by enzyme-linked immunosorbent assay. Sera were collected from immune infertile (n = 175) and fertile (n = 237) individuals and were screened by tray agglutination tests (TAT)...
April 2016: Cell and Tissue Research
Anisha A D'Souza, Smita V Yevate, A H Bandivdekar, Padma V Devarajan
The present study evaluates the feasibility of particulate carriers of a biodegradable polymer polyethylene sebacate (PES) as an alternative to Freund's adjuvant in the design of a peptide vaccine formulation. The vaccine formulation comprised of PES and the antigen KLH conjugated 80kDa HSA peptide-1 dissolved in N-methyl-2-pyrrolidone (NMP)/NMP-water as solvent. The antigen revealed good stability and the formulations were readily syringeable. Intradermal injection of the formulations resulted in the formation of PES particulates in situ at the site of injection...
December 30, 2015: International Journal of Pharmaceutics
C E Kuster, G C Althouse
Bacteriospermia is a documented risk to reproductive performance when using extended boar semen for artificial insemination. A substantial list of bacteria have been recovered from boar semen attributed to fecal, preputial, skin, and hair microorganisms, with these and other environmental bacteria from processing areas identified in doses prepared for artificial insemination. Gram-negative bacteria are most commonly recovered from extended doses, including both Enterobacteriaceae and environmental contaminants, such as those that inhabit water purification systems...
January 1, 2016: Theriogenology
Zhu Wang, Zheng Yang, Yang-Yang Lei, Ya-Dong Zhang, Li-Da Chen, Xiao-Yan Xie, Ming-De Lu, Wei Wang
The aim of this study was to explore the principle of moblile echogenicities in epididymis in patients with a history of postvasectomy or infertility, which were reported as the characteristic sonographic sign of filarial infection.We reported a 38-year-old man presented with a 3-year history of infertility after marriage. Ultrasound imaging revealed an enlarged body in the inner left epididymis along with innumerable punctate mobile echogenicities, which showed random to-and-fro movements in the left epididymis...
August 2015: Medicine (Baltimore)
Praveen Bhandari, Vijay Prabha
BACKGROUND & OBJECTIVES: Urogenital infections of bacterial origin have a high incidence among the female population at reproductive age, affecting the fertility. Strains of Escherichia coli can colonize the vagina and replace natural microflora. Lactobacillus the predominant vaginal microorganism in healthy women, maintains the acidic vaginal pH which inhibits pathogenic microorganisms. Studies on Lactobacillus have shown that these can inhibit E. coli growth and vaginal colonization...
July 2015: Indian Journal of Medical Research
Rana M A Abdella, Hatem I Abdelmoaty, Rasha H Elsherif, Ahmed Mahmoud Sayed, Nadine Alaa Sherif, Hisham M Gouda, Ahmed El Lithy, Maged Almohamady, Mostafa Abdelbar, Ahmed Naguib Hosni, Ahmed Magdy, Youssef Ma
BACKGROUND: To study the prevalence of Chlamydia infection in women with primary and secondary unexplained infertility using ELISA technique for antibody detection and real time, fully automated PCR for antigen detection and to explore its association with circulating antisperm antibodies (ASA). METHODS: A total of 50 women with unexplained infertility enrolled in this case control study and a control group of 44 infertile women with a known cause of infertility...
2015: BMC Women's Health
Caner Baran, Wayne J Hellstrom, Suresh C Sikka
A natural disaster leading to accumulation of environmental contaminants may have substantial effects on the male reproductive system. Our aim was to compare and assess semen parameters in a normospermic population residing in the Southern Louisiana, USA area pre- and post-Hurricane Katrina. We retrospectively evaluated semen analyses data (n = 3452) of 1855 patients who attended the Tulane University Andrology/Fertility Clinic between 1999 and 2013. The study inclusion criteria were men whose semen analyses showed ≥ 1...
July 2015: Asian Journal of Andrology
Harpreet Vander, Vijay Prabha
The concept of infertility as a result of asymptomatic microbial colonization of the female reproductive tract has been neglected to date. However, increasing incidence of infertility and advanced research has drawn attention towards this idea. Many of these micro-organisms have been reported to bring about adverse changes in sperm parameters in vitro, but their in vivo potential to cause infertility is still a controversy. The present study was carried out to observe what effect the intravaginal inoculation of sperm-agglutinating Serratia marcescens and sperm-immobilizing Candida albicans had in the reproductive tract and consequently in fertility outcome...
April 2015: Journal of Medical Microbiology
Arkadiy A Reunov, Yulia A Reunova
To highlight the ultrastructural features of transformation occurring with germplasm-related structures (GPRS), the spermatogenic cells of Xenopus laevis were studied by transmission electron microscopy and quantitative analysis. It was determined that in spermatogonia and spermatocytes, the compact germinal granules underwent fragmentation into particles comparable with inter-mitochondrial cement (IMC). Fragments of IMC agglutinated some cell mitochondria and resulted in the creation of mitochondrial clusters...
February 2016: Zygote: the Biology of Gametes and Early Embryos
Shengli Lin, Rong Li, Shuo Huang, Lianming Zhao, Ming Li, Junsheng Li, Jinliang Zhu, Xiaoying Zheng, Jin Huang, Ping Liu, Jie Qiao
An increasing number of infertile syphilis-infected individuals have turned to assisted reproductive technology; however, the safety of syphilis carrier serostatus on IVF and embryo transfer outcomes has not been evaluated. Data from 482 patients who delivered singletons were analysed. In the retrospective study, the rate of IVF and intracytoplasmic sperm injection fertilization was 79.50% ± 17.57%/78.72% ± 16.66% in the Treponema pallidum particle agglutination assay negative (TPPA-negative) and rapid plasma reagin negative (RPR-negative) group, 76...
December 2014: Reproductive Biomedicine Online
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