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Urine metabolic screen

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https://www.readbyqxmd.com/read/29314750/phase-i-metabolism-of-the-recently-emerged-synthetic-cannabinoid-cumyl-pegaclone-and-detection-in-human-urine-samples
#1
Lukas Mogler, Maurice Wilde, Laura M Huppertz, Georg Weinfurtner, Florian Franz, Volker Auwärter
Indole, indazole or azaindole based synthetic cannabinoids (SCs), bearing a cumyl substituent are a widespread, recreationally used subgroup of new psychoactive substances (NPS). The latest cumyl-derivative, CUMYL-PEGACLONE, emerged in December 2016 on the German drug market. The substance features a novel γ-carboline core structure, which is most likely synthesized to bypass generic legislative approaches to control SCs by prohibiting distinct core structures. Using liquid chromatography tandem mass spectrometry and liquid chromatography high resolution mass spectrometry techniques, the main in vivo phase I metabolites of this new substance were detected...
January 3, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29314710/different-in-vitro-and-in-vivo-tools-for-elucidating-the-human-metabolism-of-alpha-cathinone-derived-drugs-of-abuse
#2
Sascha K Manier, Lilian H J Richter, Jan Schäper, Hans H Maurer, Markus R Meyer
In vitro and in vivo experiments are widely used for studying the metabolism of new psychoactive substances (NPS). The availability of such data is required for toxicological risk assessments and development of urine screening approaches. This study investigated the in vitro metabolism of the five pyrrolidinophenone-derived NPS alpha-pyrrolidinobutyrophenone (alpha-PBP), alpha-pyrrolidinopentiothiophenone (alpha-PVT), alpha-pyrrolidinohexanophenone (alpha-PHP), alpha-pyrrolidinoenanthophenone (alpha-PEP, PV8), and alpha-pyrrolidinooctanophenone (alpha-POP, PV9)...
January 4, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29307017/clinical-characteristics-and-mutation-analysis-of-five-chinese-patients-with-maple-syrup-urine-disease
#3
Xiaomei Li, Yali Yang, Qing Gao, Min Gao, Yvqiang Lv, Rui Dong, Yi Liu, Kaihui Zhang, Zhongtao Gai
Maple syrup urine disease (MSUD) is an autosomal recessive disorder affecting branched-chain amino acids (BCAAs) metabolism and caused by a defect in the thiamine-dependent enzyme branched chain α-ketoacid dehydrogenase (BCKD) with subsequent accumulation of BCAAs and corresponding branched-chain keto acids (BCKAs) metabolites. Presently, at least 4 genes of BCKDHA, BCKDHB, DLD and DBT have been reported to cause MSUD. Furthermore, more than 265 mutations have been identified as the cause across different populations worldwide...
January 6, 2018: Metabolic Brain Disease
https://www.readbyqxmd.com/read/29279830/homocystinuria-with-stroke-and-positive-familial-history
#4
Ali Mazaheri, Neda Mostofizadeh, Mahin Hashemipour
Homocystinuria is the second most common treatable aminoacidopathy. Clinically, affected patients present with eye, skeleton, central nervous system, and most importantly, vascular system abnormalities. This autosomal recessive disorder leads to accumulation of homocysteine and its metabolites in the blood and urine. In this report, we present a case with clinical and biochemical findings of homocystinuria with stroke and a positive familial history of the disease in her brother. A 4-year-old girl was admitted to pediatric emergency ward because of acute onset of right hemiparesis and subsequent generalized tonic-clonic seizures...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/29237523/-clinical-analysis-of-15%C3%A2-851-children-at-risk-of-inherited-metabolic-diseases
#5
Shu-Xiang Lin, Jian-Bo Shu, Chao Wang, Rui Pan, Ying-Tao Meng, Chun-Hua Zhang, Bi-Li Zhang, Dan Wang, Yu-Qin Zhang
OBJECTIVE: To explore the value of urine gas chromatography-mass spectrometry (GC-MS) in the screening of children at risk of inherited metabolic diseases (IMD), and to identify the disease spectrum of IMD and the clinical characteristics of children with IMD. METHODS: The clinical data of 15 851 children at risk of IMD who underwent urine GC-MS in the Tianjin Children's Hospital between February 2012 and December 2016 were retrospectively analyzed. RESULTS: In the 15 851 children, 5 793 (36...
December 2017: Zhongguo Dang Dai Er Ke za Zhi, Chinese Journal of Contemporary Pediatrics
https://www.readbyqxmd.com/read/29228468/characterization-of-forsythoside-a-metabolites-in-rats-by-a-combination-of-uhplc-ltq-orbitrap-mass-spectrometer-with-multiple-data-processing-techniques
#6
Fei Wang, Guang-Shang Cao, Yun Li, Lu-Lu Xu, Zhi-Bin Wang, Ying Liu, Jian-Qiu Lu, Jia-Yu Zhang
Forsythoside A (FTA), the main active constituent isolated from Fructus Forsythiae, has various biological functions including anti-oxidant, anti-viral and anti-microbial activities. However, while researches on FTA have been mainly focused on the treatment of diseases on material basis, FTA metabolites in vivo have not been comprehensively evaluated. Here, a rapid and sensitive method using UHPLC-LTQ-Orbitrap mass spectrometer with multiple data processing techniques including high-resolution extracted ion chromatograms (HREICs), multiple mass defect filters (MMDFs) and diagnostic product ions (DPIs) was developed for screening and identification of FTA metabolites in rats...
December 11, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29214049/latent-autoimmune-diabetes-of-adulthood-case-report
#7
Cristen P Page, Brian Fitzgerald, Emily M Hawes
Background: Primary care clinicians will see a higher incidence of type 2 diabetes in adult patients, and the diagnosis and management of an initial presentation of type 1 diabetes can pose challenges to clinicians who see it less frequently. Symptoms of hyperglycemia and risk of ketoacidosis may be missed. Further, endocrine autoimmune disease can run together in patients and families. Case presentation: A 49-year-old Caucasian female with history of pituitary adenoma and Graves' disease with history of thyroid ablation presented in the outpatient setting due to hand tingling of her right middle finger that was worse in the mornings and improved throughout the day...
2017: Clinical Diabetes and Endocrinology
https://www.readbyqxmd.com/read/29179593/de-novo-identification-of-specific-exposure-biomarkers-of-the-alternative-plasticizer-di-2-ethylhexyl-terephthalate-dehtp-after-low-oral-dosage-to-male-volunteers-by-hplc-q-orbitrap-ms
#8
Frederik Lessmann, Daniel Bury, Tobias Weiss, Heiko Hayen, Thomas Brüning, Holger M Koch
CONTEXT: Human exposure biomonitoring relies on the availability of specific, sensitive biomarkers. For emerging chemicals, the identification (prediction, synthesis, verification) of such biomarkers is time and cost intensive. OBJECTIVE: This study aimed to further elucidate the urinary metabolic profile of the plasticizer di(2-ethylhexyl) terephthalate (DEHTP) in search of probably additional biomarkers of exposure. MATERIALS AND METHODS: Urine samples of an oral low dose volunteer study were analysed by HPLC-Q-Orbitrap-MS combined with a commercial data mining software...
November 28, 2017: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://www.readbyqxmd.com/read/29178637/allelic-spectrum-of-formiminotransferase-cyclodeaminase-gene-variants-in-individuals-with-formiminoglutamic-aciduria
#9
Ramanath Majumdar, Andrew Yori, Peggy W Rush, Kimiyo Raymond, Dimitar Gavrilov, Silvia Tortorelli, Dietrich Matern, Piero Rinaldo, Gerald L Feldman, Devin Oglesbee
BACKGROUND: Elevated plasma and urine formiminoglutamic acid (FIGLU) levels are commonly indicative of formiminoglutamic aciduria (OMIM #229100), a poorly understood autosomal recessive disorder of histidine and folate metabolism, resulting from formiminotransferase-cyclodeaminase (FTCD) deficiency, a bifunctional enzyme encoded by FTCD. METHODS: In order to further understanding about the molecular alterations that contribute to FIGLU-uria, we sequenced FTCD in 20 individuals with putative FTCD deficiency and varying laboratory findings, including increased FIGLU excretion...
November 2017: Molecular Genetics & Genomic Medicine
https://www.readbyqxmd.com/read/29175697/in-vivo-study-of-erysolin-metabolic-profile-by-ultra-high-performance-liquid-chromatography-coupleded-to-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#10
Binglong Li, Hui Zhou, Guochun Yang, Fei Han, Yanting Li, Yongfeng Gao, Jinwei Gao, Feng Zhang, Lixin Sun
An ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was developed for the first time to study the in vivo metabolism of erysolin, a compound derived from cruciferous plants which has a definite effect of anti-tumor and anti-nerve injury. In this research, the chromatographic separation was performed on an ACQUITY UPLC® BEH C18 column (2.1 mm×100mm, 1.7μm, Waters, USA) and eluted by a gradient program, the identification work was achieved on a Bruker ultra-high resolution spectrometer in positive ion mode...
November 16, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29168138/metabolomic-analysis-of-the-toxic-effect-of-chronic-exposure-of-cadmium-on-rat-urine
#11
Shuai Chen, Meiyan Zhang, Lu Bo, Siqi Li, Liyan Hu, Xiujuan Zhao, Changhao Sun
This study aimed to assess the toxic effect of chronic exposure to cadmium through a metabolomic approach based on ultra-performance liquid chromatography/mass spectrometry (UPLC-MS). Forty male Sprague-Dawley rats were randomly assigned to the following groups: control, low-dose cadmium chloride (CdCl2) (0.13 mg/kg body weight (bw)), middle-dose CdCl2 (0.8/kg bw), and high-dose CdCl2 (4.9 mg/kg bw). The rats continuously received CdCl2 via drinking water for 24 weeks. Rat urine samples were then collected at different time points to establish the metabolomic profiles...
November 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29157190/mucopolysaccharidosis-type-vi-in-a-great-dane-caused-by-a-nonsense-mutation-in-the-arsb-gene
#12
Ping Wang, Carol Margolis, Gloria Lin, Elizabeth L Buza, Scott Quick, Karthik Raj, Rachel Han, Urs Giger
Mucopolysaccharidoses are inherited metabolic disorders that result from a deficiency of lysosomal enzymes required for the catabolism of glycosaminoglycans. Lysosomal glycosaminoglycan accumulation results in cell and organ dysfunction. This study characterized the phenotype and genotype of mucopolysaccharidosis VI in a Great Dane puppy with clinical signs of stunted growth, facial dysmorphia, skeletal deformities, corneal opacities, and increased respiratory sounds. Clinical and pathologic evaluations, urine glycosaminoglycan analyses, lysosomal enzyme assays, and ARSB sequencing were performed...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/29153022/-rhabdomyolysis-may-it-be-a-metabolic-myopathy-case-report-and-diagnostic-algorithm
#13
Ágnes Sebők, Endre Pál, Gergő Attila Molnár, István Wittmann, Judit Berenténé Bene, Béla Melegh, Sámuel Komoly, Tibor Hidvégi, Lídia Balogh, Attila Szabó, Petra Zsidegh
We report the case of a 46-year-old female patient with recurrent rhabdomyolysis. In the background of her metabolic myopathy an inherited metabolic disorder of the fatty acid oxidation, very long-chain acyl-coenzyme A-dehydrogenase deficiency was diagnosed. The diagnosis was based on abnormal acyl-carnitine- and urine organic-acid profile in addition to low residual enzyme activity, and was confirmed by genetic testing. After introduction of dietotherapy metabolic crisis necessitating hospital admission has not occurred neither have fixed myopathic changes developed...
November 2017: Orvosi Hetilap
https://www.readbyqxmd.com/read/29148594/metabolic-study-of-paeoniflorin-and-total-paeony-glucosides-from-paeoniae-radix-rubra-in-rats-by-high-performance-liquid-chromatography-coupled-with-sequential-mass-spectrometry-uplc-esi-ms-n
#14
Lijun Zhu, Shanshan Sun, Yanxi Hu, Yufeng Liu
A clear understanding of the metabolism of Traditional Chinese Medicine (TCM) is extremely important in their rational clinical application and effective material foundation research. A novel and reliable strategy was performed to find more metabolites of paeoniflorin, determine the metabolites of total paeony glucosides (TPG) by means of determining those metabolites of paeoniflorin, and compare the metabolism differences between paeoniflorin and TPG by intragastric administration. This strategy was characterized as follows: firstly, the rats were divided into two groups (the paeoniflorin group and the TPG group) to find different metabolism mechanism between paeoniflorin and TPG; secondly, UPLC-FT-ICR MS and UPLC-Q-TOF MS(2) were applied to obtain accurate molecular weight and structural information respectively; thirdly, the metabolites were tentatively identified by a combination of data-processing methods including mass defect screening, characteristic neutral loss screening and product ion screening; finally, a comparative study was employed in the metabolism of paeoniflorin and TPG...
November 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29131975/identification-of-urinary-biomarkers-of-exposure-to-di-2-propylheptyl-phthalate-using-high-resolution-mass-spectrometry-and-two-data-screening-approaches
#15
Chia-Lung Shih, Pao-Mei Liao, Jen-Yi Hsu, Yi-Ning Chung, Victor G Zgoda, Pao-Chi Liao
Di-(2-propylheptyl) phthalate (DPHP) is a plasticizer used in polyvinyl chloride and vinyl chloride copolymer that has been suggested to be a toxicant in rats and may affect human health. Because the use of DPHP is increasing, the general German population is being exposed to DPHP. Toxicant metabolism is important for human toxicant exposure assessments. To date, the knowledge regarding DPHP metabolism has been limited, and only four metabolites have been identified in human urine. Ultra-performance liquid chromatography was coupled with Orbitrap high-resolution mass spectrometry (MS) and two data-screening approaches-the signal mining algorithm with isotope tracing (SMAIT) and the mass defect filter (MDF)-for DPHP metabolite candidate discovery...
October 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/29131518/metabolic-profile-of-kudiezi-injection-in-rats-by-uhplc-coupled-with-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#16
Jingdan Zhang, Xiaoxue Zhang, Yangyang Zhao, Aihua Song, Wei Sun, Ran Yin
In this study, a reliable and sensitive ultra high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry method was developed for the systematic study of the metabolic profile of Kudiezi injection in rat plasma, bile, urine and feces after intravenous administration of a single dose. The chromatographic separation was performed on an Agilent Eclipse Plus C18 column (4.6 mm × 50 mm, 1.8 μm) and the identification of prototype components and metabolites was achieved on a Bruker Solarix 7...
November 13, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/29128830/metabolism-of-the-synthetic-cannabinoid-5f-py-pica-by-human-and-rat-hepatocytes-and-identification-of-biliary-analytical-targets-by-directional-efflux-in-sandwich-cultured-rat-hepatocytes-using-uhplc-hr-ms-ms
#17
Marie Mardal, Pieter Annaert, Carolina Noble, Marlies Oorts, Kristian Linnet
Analytical strategies for detecting drugs in biological samples rely on information on metabolism and elimination. 5F-PY-PICA belongs to the group of synthetic cannabinoids that are known to undergo excretion into the bile. The aims of this study were the in vitro identification of metabolites of 5F-PY-PICA and to determine which analytical targets are excreted into the bile and urine. Metabolites identified after incubation of 5F-PY-PICA with pooled human liver microsomes (pHLM), pooled human hepatocytes (pHH), or suspended and sandwich-cultured rat hepatocytes (SCRH)...
November 7, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29110414/clinical-characteristics-of-schoolchildren-with-renal-glucosuria
#18
Tastuhiko Urakami, Midori Yoda, Kei Yoshida, Yusuke Mine, Masako Aoki, Junichi Suzuki
BACKGROUND: We have conducted an annual urine glucose screening program at schools, and diagnosed schoolchildren with diabetes at an early stage of the disease. We also identified some cases with renal glucosuria (RG), based on positive urine glucose with normal glucose tolerance. METHODS: During the period from 2000 to 2015, 3, 309, 631 schoolchildren participated in the screening program. The positive rate for glucosuria in the first test was approximately 0.1%, whereas in the second test it was approximately 0...
November 7, 2017: Pediatrics International: Official Journal of the Japan Pediatric Society
https://www.readbyqxmd.com/read/29105858/investigation-of-metabolic-profile-of-pimavanserin-in-rats-by-uhplc-combined-with-fourier-transform-ion-cyclotron-resonance-mass-spectrometry
#19
Wenying Wu, Yanjie Chu, Shixiao Wang, Xiaoyang Sun, Jingjing Zhang, Yannian Wang, Xiaohui Chen
RATIONALE: Pimavanserin, a selective serotonin 2A receptor inverse agonist, is a promising candidate for treating Parkinson's disease psychosis. Our previous study exhibited that there might be an existence of extensive metabolites of pimavanserin in rats. However, the metabolic fate of pimavanserin in vivo remains unknown. Thus, it is essential to develop an efficient method to investigate metabolic profile of pimavanserin in rats. Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) to date has the highest mass measurement accuracy and resolution of any mass spectrometry platforms...
November 6, 2017: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29094226/aminoacidopathies-prevalence-etiology-screening-and-treatment-options
#20
REVIEW
Muhammad Wasim, Fazli Rabbi Awan, Haq Nawaz Khan, Abdul Tawab, Mazhar Iqbal, Hina Ayesha
Inborn errors of metabolism (IEMs) are a group of inherited metabolic disorders which are caused by mutations in the specific genes that lead to impaired proteins or enzymes production. Different metabolic pathways are perturbed due to the deficiency or lack of enzymes. To date, more than 500 IEMs have been reported with most of them being untreatable. However, fortunately 91 such disorders are potentially treatable, if diagnosed at an earlier stage of life. IEMs have been classified into different categories and one class of IEMs, characterized by the physiological disturbances of amino acids is called as aminoacidopathies...
November 1, 2017: Biochemical Genetics
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