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https://www.readbyqxmd.com/read/28214442/hydrothermal-liquefaction-of-sewage-sludge-under-isothermal-and-fast-conditions
#1
Lili Qian, Shuzhong Wang, Phillip E Savage
We investigated the effects of water loading, sludge moisture content, recovery solvent, and additives on the product yields and compositions from isothermal (673K, 60min) and fast (773K, 1min) hydrothermal liquefaction (HTL) of sewage sludge. The water loading (which affects pressure within the reactor) plays a small role in product yields. The sludge moisture content had a larger effect with the highest biocrude yields (26.8wt% from isothermal HTL and 27.5wt% from fast HTL) being produced from sludge that was 85wt% moisture...
February 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214439/different-cultivation-methods-to-acclimatise-ammonia-tolerant-methanogenic-consortia
#2
Hailin Tian, Ioannis A Fotidis, Enrico Mancini, Irini Angelidaki
Bioaugmentation with ammonia tolerant-methanogenic consortia was proposed as a solution to overcome ammonia inhibition during anaerobic digestion process recently. However, appropriate technology to generate ammonia tolerant methanogenic consortia is still lacking. In this study, three basic reactors (i.e. batch, fed-batch and continuous stirred-tank reactors (CSTR)) operated at mesophilic (37°C) and thermophilic (55°C) conditions were assessed, based on methane production efficiency, incubation time, TAN/FAN (total ammonium nitrogen/free ammonia nitrogen) levels and maximum methanogenic activity...
February 11, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28214390/robust-peptidoglycan-growth-by-dynamic-and-variable-multi-protein-complexes
#3
REVIEW
Manuel Pazos, Katharina Peters, Waldemar Vollmer
In Gram-negative bacteria such as Escherichia coli the peptidoglycan sacculus resides in the periplasm, a compartment that experiences changes in pH value, osmolality, ion strength and other parameters depending on the cell's environment. Hence, the cell needs robust peptidoglycan growth mechanisms to grow and divide under different conditions. Here we propose a model according to which the cell achieves robust peptidoglycan growth by employing dynamic multi-protein complexes, which assemble with variable composition from freely diffusing sets of peptidoglycan synthases, hydrolases and their regulators, whereby the composition of the active complexes depends on the cell cycle state - cell elongation or division - and the periplasmic growth conditions...
February 15, 2017: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/28214347/involvement-of-the-guanine-nucleotide-exchange-factor-vav3-in-central-nervous-system-development-and-plasticity
#4
Annika Ulc, Christine Gottschling, Ina Schäfer, David Wegrzyn, Simon van Leeuwen, Veronika Luft, Jacqueline Reinhard, Andreas Faissner
Small GTP-hydrolysing enzymes (GTPases) of the RhoA family play manifold roles in cell biology and are regulated by upstream guanine nucleotide exchange factors (GEFs). Herein, we focus on the GEFs of the Vav subfamily. Vav1 was originally described as a protooncogene of the hematopoietic lineage. The GEFs Vav2 and Vav3 are more broadly expressed in various tissues. In particular, the GEF Vav3 may play important roles in the developing nervous system during the differentiation of neural stem cells into the major lineages, namely neurons, oligodendrocytes and astrocytes...
February 18, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28214337/palmitate-induces-mitochondrial-superoxide-generation-and-activates-ampk-in-podocytes
#5
Eugene Lee, Jin Choi, Hyun Soon Lee
Studies have shown that high levels of serum free fatty acids (FFAs) are associated with lipotoxicity and type 2 diabetes. Palmitic acid (PA) is the predominant circulating saturated FFA, yet its role in the pathogenesis of diabetic nephropathy (DN) is not clear. Recently, one study suggested that mitochondrial superoxide production is related to AMP-activated protein kinase (AMPK) activity in diabetic mice kidneys. To elucidate the link between PA and oxidative stress and AMPK activity in DN, we compared the cultured murine podocytes exposed to PA and oleic acid (OA)...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28214336/evidence-to-show-that-acetonitrile-is-sensitive-to-different-interactions-sites-of-ionic-liquids-as-unveiled-by-excess-spectroscopy
#6
Yu Zhou, Yan-Zhen Zheng, Tian Zhang, Geng Deng, Zhi-Wu Yu
By studying the interactions between an ionic liquid (IL), 1-ethyl-3-methylimidazolium bis (trifluoromethylsulfonyl) imide ([Emim][Tf2N]) and a co-solvent acetonitrile, C≡N stretching vibration was found to be very sensitive to different interaction sites as unveiled by excess infrared spectroscopy. Four existing forms of acetonitrile molecules were identified and the detailed transformation process of the ionic liquid upon dilution was obtained. Such characteristic of nitrile group is discussed from the view point of its ability to form hydrogen bonds with proton donors...
February 18, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28214242/injury-and-recovery-of-escherichia-coli-atcc25922-cells-treated-by-high-hydrostatic-pressure-at-400-600%C3%A2-mpa
#7
Keitarou Kimura, Kazuya Morimatsu, Takashi Inaoka, Kazutaka Yamamoto
Escherichia coli cells were inactivated by high hydrostatic pressure (HHP) at 400-600 MPa and their recovery under various conditions was evaluated by colony counting and flow cytometer (FCM) analyses. The lag time in colony formation and an improved recovery of cells under less oxidative conditions (pyruvate addition to the medium and incubation in anaerobic conditions) were observed for HHP treated cells, which indicated that a significant portion of cells were injured and recovered during incubation after HHP treatment...
February 14, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28214122/anaerobic-co-digestion-of-recalcitrant-agricultural-wastes-characterizing-of-biochemical-parameters-of-digestate-and-its-impacts-on-soil-ecosystem
#8
Adele Muscolo, Giovanna Settineri, Teresa Papalia, Emilio Attinà, Carmelo Basile, Maria Rosaria Panuccio
Anaerobic digestion (AD) of organic wastes is a promising alternative to landfilling for reducing Greenhouse Gas Emission (GHG) and it is encouraged by current regulation in Europe. Biogas-AD produced, represents a useful source of green energy, while its by-product (digestate) is a waste, that needs to be safely disposal. The sustainability of anaerobic digestion plants partly depends on the management of their digestion residues. This study has been focused on the environmental and economic benefits of co-digest recalcitrant agricultural wastes such olive wastes and citrus pulp, in combination with livestock wastes, straw and cheese whey for biogas production...
February 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28214121/a-molecular-zoom-into-soil-humeome-by-a-direct-sequential-chemical-fractionation-of-soil
#9
Marios Drosos, Antonio Nebbioso, Pierluigi Mazzei, Giovanni Vinci, Riccardo Spaccini, Alessandro Piccolo
A Humeomics sequential chemical fractionation coupled to advanced analytical identification was applied directly to soil for the first time. Humeomics extracted ~235% more soil organic carbon (SOC) than by the total alkaline extraction traditionally employed to solubilise soil humic molecules (soil Humeome). Seven fractions of either hydro- or organo-soluble components and a final unextractable humic residue were separated from soil. These materials enabled an unprecedented structural identification of solubilised heterogeneous humic molecules by combining NMR, GC-MS, and ESI-Orbitrap-MS...
February 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28214116/response-of-fish-communities-to-multiple-pressures-development-of-a-total-anthropogenic-pressure-intensity-index
#10
Sandra Poikane, David Ritterbusch, Christine Argillier, Witold Białokoz, Petr Blabolil, Jan Breine, Nicolaas G Jaarsma, Teet Krause, Jan Kubečka, Torben L Lauridsen, Peeter Nõges, Graeme Peirson, Tomas Virbickas
Lakes in Europe are subject to multiple anthropogenic pressures, such as eutrophication, habitat degradation and introduction of alien species, which are frequently inter-related. Therefore, effective assessment methods addressing multiple pressures are needed. In addition, these systems have to be harmonised (i.e. intercalibrated) to achieve common management objectives across Europe. Assessments of fish communities inform environmental policies on ecological conditions integrating the impacts of multiple pressures...
February 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28214085/folic-acid-protects-against-lead-acetate-induced-hepatotoxicity-by-decreasing-nf-%C3%AE%C2%BAb-il-1%C3%AE-production-and-lipid-peroxidation-mediataed-cell-injury
#11
Eman S H Abd Allah, Dalia M Badary
Folic acid plays an important role in cellular metabolic activities. The present study was designed to investigate the protective effect of folic acid against lead acetate-induced hepatotoxicity. Twenty four male Wistar albino rats were randomly divided into four groups, six animals each. Negative control group received the vehicle, positive control group received 1mg/kg folic acid for five consecutive days/week for 4 weeks orally, lead-exposed group received 10mg/kg lead acetate intraperitoneally (IP) for five consecutive days/week for 4 weeks, and lead-treated group received 10mg/kg lead acetate IP and 1mg/kg folic acid orally for five consecutive days/week for 4 weeks concurrently...
February 10, 2017: Pathophysiology: the Official Journal of the International Society for Pathophysiology
https://www.readbyqxmd.com/read/28214059/pharmacokinetic-alterations-of-rhubarb-anthraquinones-in-experimental-colitis-induced-by-dextran-sulfate-sodium-in-the-rat
#12
Wen-Jin Wu, Ru Yan, Ting Li, Ya-Ping Li, Rui-Na Zhou, Yi-Tao Wang
ETHNOPHARMACOLOGICAL RELEVANCE: Rhubarb (Rhei Rhizoma et Radix) is used for the treatment of digestive diseases in traditional medicinal practice in China. Recent studies also support its beneficial activities in alleviating ulcerative colitis (UC). AIM OF THE STUDY: This study aimed to characterize the oral pharmacokinetics of rhubarb anthraquinones, the main bioactive components of this herb, in the experimental chronic colitis rat model induced by dextran sulfate sodium (DSS) and to identify the factors causing the pharmacokinetic alterations...
February 14, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28213992/hydrolyzable-tannins-from-the-fruits-of-terminalia-chebula-retz-and-their-%C3%AE-glucosidase-inhibitory-activities
#13
Dong Young Lee, Hyun Woo Kim, Heejung Yang, Sang Hyun Sung
Nine hydrolyzable tannins, including three previously unknown and six artifacts, were isolated, together with thirty-nine known ones, from the fruits of Terminalia chebula Retz. (Combretaceae). They were identified as 1,2,3-tri-O-galloyl-6-O-cinnamoyl-β-d-glucose, 1,2,3,6-tetra-O-galloyl-4-O-cinnamoyl-β-d-glucose, 4-O-(2″,4″-di-O-galloyl-α-l-rhamnosyl)ellagic acid, 1'-O-methyl neochebulanin, dimethyl neochebulinate, 6'-O-methyl neochebulagate, dimethyl neochebulagate, dimethyl 4'-epi-neochebulagate, and methyl chebulagate by the spectroscopic interpretation...
February 14, 2017: Phytochemistry
https://www.readbyqxmd.com/read/28213986/investigation-of-the-temperature-dependence-of-water-adsorption-on-silica-based-stationary-phases-in-hydrophilic-interaction-liquid-chromatography
#14
Endre Bartó, Attila Felinger, Pavel Jandera
In the present work, the adsorption of water was investigated in aqueous normal-phase liquid chromatography on Cogent Silica C and Cogent Phenyl hydride stationary phases at different temperatures by frontal analysis - using coulometric Karl Fischer titration - to compare the temperature dependence of adsorption of water from aqueous acetonitrile. The Cogent Silica-C and Cogent Phenyl Hydride columns have a silicon hydride surface (silica hydride) with less than 2% free silanol group; therefore, they do not have a strong association with water...
February 9, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28213972/promotion-of-cell-growth-and-adhesion-of-a-peptide-hydrogel-scaffold-via-mtor-cadherin-signaling
#15
Guojun Wei, Liping Wang, Daming Dong, Zhaowei Teng, Zuowei Shi, Kaifu Wang, Gang An, Ying Guan, Bo Han, Meng Yao, Cory J Xian
Understanding neurite outgrowth, orientation, and migration is important for the design of biomaterials that interface with the neural tissue. However, the molecular signaling alternations have not been well elucidated to explain the impact of hydrogels on cell morphology. In our previous studies, a silk fibroin peptide (SF16) hydrogel was found to be an effective matrix for the viability, morphology and proliferation of PC12 rat pheocrhomocytoma cells. We found that PC12 cells in the peptide hydrogel exhibited adhesive morphology compared to those cultured in agarose or collagen...
February 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28213949/peri-implant-bone-remodeling-at-the-interface-of-three-different-implant-types-a-histomorphometric-study-in-mini-pigs
#16
Wenjuan Zhou, Sonja Kuderer, Zhonghao Liu, Christian Ulm, Xiaohui Rausch-Fan, Stefan Tangl
OBJECTIVES: The aim of this study was to perform a histomorphometric evaluation of the healing characteristics and jawbone remodeling processes in the peri-implant bone tissue of three commonly used implant surfaces, during the early weeks of osseointegration. MATERIAL AND METHODS: Dental implants with three different surfaces (anodically modified, hydroxyapatite (HA)-coated and machined) were inserted into the mandibular side-tooth region of twelve adult mini-pigs...
February 18, 2017: Clinical Oral Implants Research
https://www.readbyqxmd.com/read/28213944/separation-of-hydroxynorketamine-stereoisomers-using-capillary-electrophoresis-with-sulfated-%C3%AE-cyclodextrin-and-highly-sulfated-%C3%AE-cyclodextrin
#17
Friederike A Sandbaumhüter, Regula Theurillat, Wolfgang Thormann
The racemic N-methyl-d-aspartate receptor antagonist ketamine is used in anesthesia, analgesia and the treatment of depressive disorders. It is known that interactions of hydroxylated norketamine metabolites and 5,6-dehydronorketamine (DHNK) with the α7 -nicotinic acetylcholine receptor and the α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor are responsible for the antidepressive effects. Ketamine and its first metabolite norketamine are not active on these receptors. As stereoselectivity plays a role in ketamine metabolism, a cationic capillary electrophoresis based method capable of resolving and analyzing the stereoisomers of four hydroxylated norketamine metabolites, norketamine and DHNK was developed...
February 18, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28213937/targeting-phosphodiesterase-5-as-a-therapeutic-option-against-myocardial-ischemia-reperfusion-injury-and-in-treating-heart-failure
#18
REVIEW
Sevil Korkmaz-Icöz, Tamás Radovits, Gábor Szabó
Phosphodiesterase type 5 (PDE5) selectively hydrolyses the second messenger cyclic 3´-5´guanosine monophosphate (cGMP) into 5'-GMP, thereby regulating its intracellular concentrations. Dysregulation of the cGMP-dependent pathway plays a significant role in various cardiovascular diseases. Therefore, its modulation by drugs, such as PDE5 inhibitors, may represent an effective therapeutic approach. There are currently four PDE5 inhibitors available for the treatment of erectile dysfunction: sildenafil, vardenafil, tadalafil, and avanafil...
February 18, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28213905/a-ten-gene-containing-genomic-island-determines-flagellin-glycosylation-implication-for-its-regulatory-role-in-motility-and-virulence-of-xanthomonas-oryzae-pv-oryzae
#19
Chao Yu, Huamin Chen, Fang Tian, Fenghuan Yang, Xiaochen Yuan, Ching-Hong Yang, Chenyang He
Flagellin glycosylation plays crucial roles in flagellar assembly, motility and virulence in several pathogenic bacteria. However, little is known about the genetic determinants and biological functions of flagellin glycosylation in Xanthomonas oryzae pv. oryzae (Xoo), the causal pathogen of bacterial blight of rice. Here, the structure, regulation and functions of a ten-gene cluster gigX (glycosylation island genes of Xoo), which was embedded in a flagellar regulon were characterized. gigX1 through gigX10, encoded putative enzymes or proteins involved in glycan biosynthesis and transfer, including a nucleotide sugar transaminase, an acyl-carrier protein (ACP), a 3-oxoacyl-ACP synthase, a 3-oxoacyl-ACP reductase, a dehydrogenase, an acetyltransferase, a ring hydroxylating dioxygenase, a hypothetical protein, a methyltransferanse, and a glycosyltransferase, respectively...
February 18, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28213894/controllable-rotating-behavior-of-individual-dielectric-micro-rod-in-a-rotating-electric-field
#20
Weiyu Liu, Yukun Ren, Ye Tao, Yanbo Li, Xiaoming Chen
We report herein controllable rotating behavior of an individual dielectric micro-rod driven by a background rotating electric field. By disposing or removing structured floating microelectrode, the rigid rod suspended in electrolyte solution accordingly exhibits co-field or anti-field rotating motion. In the absence of the ideally polarizable metal surface, the dielectric rod rotates opposite to propagation of electric field, with the measured rotating rate much larger than predicted by Maxwell-Wager interfacial polarization theory incorporating surface conduction of fixed bond charge...
February 18, 2017: Electrophoresis
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