keyword
MENU ▼
Read by QxMD icon Read
search

perforin

keyword
https://www.readbyqxmd.com/read/29040297/decreased-nk-cell-immunity-in-kidney-transplant-recipients-late-post-transplant-and-increased-nk-cell-immunity-in-patients-with-recurrent-miscarriage
#1
Li Zhu, Mostafa Aly, Haihao Wang, Hristos Karakizlis, Rolf Weimer, Christian Morath, Ruben Jeremias Kuon, Bettina Toth, Gerhard Opelz, Volker Daniel
BACKGROUND: There is evidence that NK-cell reactivity might affect graft outcome in transplant recipients and pregnancy in women. METHOD: NK-cell subsets were determined in whole blood using eight-colour-fluorescence flow cytometry in patients before and after renal transplantation, patients with recurrent miscarriage (RM) and healthy controls (HC). RESULTS: Patients late post-transplant (late-Tx) with functioning renal transplants showed abnormally low CD56dimCD16+ NK-cells containing both perforin and granzyme (vs HC p = 0...
2017: PloS One
https://www.readbyqxmd.com/read/29027004/blood-lymphocyte-subsets-identify-optimal-responders-to-ifn-beta-in-ms
#2
Raquel Alenda, Lucienne Costa-Frossard, Roberto Alvarez-Lafuente, Carmen Espejo, Eulalia Rodríguez-Martín, Susana Sainz de la Maza, Noelia Villarrubia, Jordi Río, María I Domínguez-Mozo, Xavier Montalban, José C Álvarez-Cermeño, Luisa M Villar
Response to interferon-beta (IFN-beta) treatment is heterogeneous in multiple sclerosis (MS). We aimed to search for biomarkers predicting no evidence of disease activity (NEDA) status upon IFN-beta treatment in MS. 119 patients with relapsing-remitting MS (RRMS) initiating IFN-beta treatment were included in the study, and followed prospectively for 2 years. Neutralizing antibodies (NAb) were explored in serum samples obtained after 6 and 12 months of IFN-beta treatment. Soluble cytokines and blood lymphocytes were studied in basal samples by ELISA and flow cytometry, respectively...
October 12, 2017: Journal of Neurology
https://www.readbyqxmd.com/read/29017458/lifestyle-gene-gain-and-loss-and-transcriptional-remodeling-cause-divergence-in-the-transcriptomes-of-phytophthora-infestans-and-pythium-ultimum-during-potato-tuber-colonization
#3
Audrey M V Ah-Fong, Jolly Shrivastava, Howard S Judelson
BACKGROUND: How pathogen genomes evolve to support distinct lifestyles is not well-understood. The oomycete Phytophthora infestans, the potato blight agent, is a largely biotrophic pathogen that feeds from living host cells, which become necrotic only late in infection. The related oomycete Pythium ultimum grows saprophytically in soil and as a necrotroph in plants, causing massive tissue destruction. To learn what distinguishes their lifestyles, we compared their gene contents and expression patterns in media and a shared host, potato tuber...
October 10, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28994628/effects-of-total-flavonoids-of-sea-buckthorn-hippophae-rhamnoides-l-on-cytotoxicity-of-nk92-mi-cells
#4
Diandong Hou, Decheng Wang, Xiande Ma, Wenna Chen, Shengnan Guo, Hongquan Guan
Sea buckthorn ( Hippophae rhamnoides L.) has multifarious medicinal properties including immunoregulatory effect. The total flavonoids of Hippophae rhamnoides L. (TFH) are the main active components isolated from berries of sea buckthorn. The aim of this study was to evaluate the effects of TFH on the cytotoxicity of NK92-MI cells and its possible mechanisms. NK92-MI cells were treated with TFH (2.5 or 5.0 mg/L) or phosphate-buffered saline (PBS) for 24 h, the cytotoxicity against K562 was detected by measuring the release of lactate dehydrogenase (LDH), expression levels of NCRs (NKp30, NKp44, NKp46) and NKG2D were detected by flow cytometry, and expression levels of perforin and granzyme B were detected by western blot...
October 1, 2017: International Journal of Immunopathology and Pharmacology
https://www.readbyqxmd.com/read/28986067/counterintuitive-regulatory-t-cell-responses-are-observed-in-senior-patients-with-community-acquired-pneumonia-and-are-related-to-disease-severity
#5
Lu-Qin Bian, Ying Bi, Shao-Wei Zhou, Zi-Dan Chen, Jun Wen, Jin Shi, Ling Mao, Ling Wang
Senior individuals older than 65 years of age are at a disproportionally higher risk of developing pneumonia. Impaired capacity to defend against airway infections may be one of the reasons. It is generally believed that weaker regulatory T cell responses may be beneficial to host defense against pathogens. In senior patients with community-acquired bacterial pneumonia, we investigated the frequencies and functions of regulatory T cells. Interestingly, we found that compared to age- and sex-matched healthy controls, senior pneumonia patients presented lower frequencies of Foxp3-expressing and Helios-expressing CD4(+) T cells...
October 3, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28980803/analysis-of-pore-formation-and-protein-translocation-using-large-biological-nanopores
#6
Hirokazu Watanabe, Alberto Gubbiotti, Mauro Chinappi, Natsumi Takai, Koji Tanaka, Kouhei Tsumoto, Ryuji Kawano
This paper describes the analysis of pore formation and detection of a single protein molecule using a large nanopore among five different pore-forming proteins. We demonstrate that the identification of appropriate pores for nanopore sensing can be achieved by classifying the channel current signals and performing noise analysis. Through these analyses, we selected a perforin nanopore from the membrane attack complex/perforin superfamily and attempted to use it to detect the granzyme B protein, a serine protease...
October 5, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28971893/the-crosstalk-between-esc-and-m%C3%AF-impairs-cytotoxicity-of-nk-cell-by-secreting-il-10-and-tgf-%C3%AE-in-ems
#7
Hui-Li Yang, Wen-Jie Zhou, Kai-Kai Chang, Jie Mei, Li-Qing Huang, Ming-Yan Wang, Yi Meng, Si-Yao Ha, Da-Jin Li, Ming-Qing Li
The dysfunction of NK cells in women with endometriosis (EMS) contributes to the immune escape of menstrual endometrial fragments refluxed into the peritoneal cavity. The reciprocal communications between endometrial stromal cells (ESCs) and lymphocytes facilitate the development of EMS. However, the mechanism of these communications on cytotoxicity of natural killer (NK) cells in endometriotic milieus is still larger unknown. To imitate the local immune microenvironment, the co-culture systems of ESCs from patients with EMS and monocyte-derived macrophages or of ESCs, macrophages and NK cells were constructed...
October 2, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28961609/dcone-as-an-allogeneic-cell-based-vaccine-for-multiple-myeloma
#8
Rebecca Karp Leaf, Dina Stroopinsky, Athalia R Pyzer, Ada M Kruisbeek, Sandra van Wetering, Abigail Washington, Adam Ephraim, Leandra Cole, Adam Morin, Salvia Jain, Myrna R Nahas, Arik Apel, Jon Arnason, Ayad Hamdan, Jacalyn Rosenblatt, David Avigan
Multiple myeloma (MM) is characterized by progressive immune dysregulation, loss of myeloma-specific immunity, and an immunosuppressive milieu that fosters disease growth and immune escape. Accordingly, cancer vaccines that reverse tumor-associated immune suppression represent a promising therapeutic avenue of investigation. We examined the potential of an allogeneic cellular vaccine to generate immune responses against MM tumor cells. The DCOne vaccine is comprised of a human myeloid leukemia cell line differentiated into a fully functional dendritic cell, expressing a range of tumor-associated antigens that are also known targets in MM...
November 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/28959678/in-vivo-immunoprotective-role-of-indigofera-tinctoria-and-scoparia-dulcis-aqueous-extracts-against-chronic-noise-stress-induced-immune-abnormalities-in-wistar-albino-rats
#9
Boothapandi Madakkannu, Ramanibai Ravichandran
Indigofera tinctoria and Scoparia dulcis are being widely used in Indian folk medicine for the treatment of various disorders. Environmental noise pollution is thought to be an important factor for many health problems and it causes immune abnormalities. In the present study immune-regulating potential of I. tinctoria and S. dulcis aqueous extracts on innate and adaptive immune system of wistar albino rats was evaluated during normal and chronic noise induced stress conditions. The results demonstrated that both I...
2017: Toxicology Reports
https://www.readbyqxmd.com/read/28937414/rheumatoid-arthritis-and-citrullination
#10
Erika Darrah, Felipe Andrade
PURPOSE OF REVIEW: Dysregulated citrullination is a key element that drives the production and maintenance of antibodies to citrullinated proteins, a hallmark in rheumatoid arthritis (RA). This article reviews recent literature on the origin of citrullinated antigens in RA. RECENT FINDINGS: The study of synovial fluid from patients with RA has provided important insights into the identity of citrullinated proteins that accumulate in the RA joint (the RA citrullinome) and mechanisms that control their generation...
September 21, 2017: Current Opinion in Rheumatology
https://www.readbyqxmd.com/read/28926522/bos-is-associated-with-increased-cytotoxic-proinflammatory-cd8-t-nkt-like-and-nk-cells-in-the-small-airways
#11
Greg Hodge, Sandra Hodge, Aeneas Yeo, Phan Nguyen, Emily Hopkins, Chien-Li Holmes-Liew, Paul N Reynolds, Mark Holmes
BACKGROUND: Immunosuppression therapy after lung transplantation fails to prevent bronchiolitis obliterans syndrome (BOS) in many patients, primarily a disease of the small airways. We have reported that BOS is associated with a lack of suppression of cytotoxic mediators, and proinflammatory cytokines, in peripheral blood T, NKT-like (particularly CD8+) and NK cells. We also showed a loss of glucocorticoid receptor (GCR) in proinflammatory lymphocytes after transplant. It is unknown whether these proinflammatory lymphocytes target the small and/or large airways in BOS...
October 2017: Transplantation
https://www.readbyqxmd.com/read/28919991/umbilical-cord-blood-cd34-progenitor-derived-nk-cells-efficiently-kill-ovarian-cancer-spheroids-and-intraperitoneal-tumors-in-nod-scid-il2rg-null-mice
#12
Janneke S Hoogstad-van Evert, Jeannette Cany, Dirk van den Brand, Manon Oudenampsen, Roland Brock, Ruurd Torensma, Ruud L Bekkers, Joop H Jansen, Leon F Massuger, Harry Dolstra
Adoptive transfer of allogeneic natural killer (NK) cells is an attractive therapy approach against ovarian carcinoma. Here, we evaluated the potency of highly active NK cells derived from human CD34+ haematopoietic stem and progenitor cells (HSPC) to infiltrate and mediate killing of human ovarian cancer spheroids using an in vivo-like model system and mouse xenograft model. These CD56+Perforin+ HSPC-NK cells were generated under stroma-free conditions in the presence of StemRegenin-1, IL-15, and IL-12, and exerted efficient cytolytic activity and IFNγ production toward ovarian cancer monolayer cultures...
2017: Oncoimmunology
https://www.readbyqxmd.com/read/28912605/peripheral-human-cd4-cd8-t-lymphocytes-exhibit-a-memory-phenotype-and-enhanced-responses-to-il-2-il-7-and-il-15
#13
Marie-Laure Clénet, François Gagnon, Ana Carmena Moratalla, Emilie C Viel, Nathalie Arbour
CD4(+)CD8(+) T lymphocytes account for 1-2% of circulating human T lymphocytes, but their frequency is augmented in several diseases. The phenotypic and functional properties of these T lymphocytes are still ill-defined. We performed an ex vivo characterization of CD4(+)CD8(+) T lymphocytes from the blood of healthy individuals. We observed that CD4(+)CD8(+) T lymphocytes exhibit several characteristics associated with memory T lymphocytes including the expression of chemokine receptors (e.g. CCR7, CXCR3, CCR6) and activation markers (e...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28903036/culturing-ctls-under-hypoxic-conditions-enhances-their-cytolysis-and-improves-their-anti-tumor-function
#14
Yael Gropper, Tali Feferman, Tali Shalit, Tomer-Meir Salame, Ziv Porat, Guy Shakhar
Cytotoxic T lymphocytes (CTLs) used in immunotherapy are typically cultured under atmospheric O2 pressure but encounter hypoxic conditions inside tumors. Activating CTLs under hypoxic conditions has been shown to improve their cytotoxicity in vitro, but the mechanism employed and the implications for immunotherapy remain unknown. We activated and cultured OT-I CD8 T cells at either 1% or 20% O2. Hypoxic CTLs survived, as well as normoxic ones, in vitro but killed OVA-expressing B16 melanoma cells more efficiently...
September 12, 2017: Cell Reports
https://www.readbyqxmd.com/read/28881722/hepatic-nk-cell-mediated-hypersensitivity-to-cona-induced-liver-injury-in-mouse-liver-expressing-hepatitis-c-virus-polyprotein
#15
Qiuxia Fu, Shaoduo Yan, Licui Wang, Xiangguo Duan, Lei Wang, Yue Wang, Tao Wu, Xiaohui Wang, Jie An, Yulong Zhang, Qianqian Zhou, Linsheng Zhan
The role of hepatic NK cells in the pathogenesis of HCV-associated hepatic failure is incompletely understood. In this study, we investigated the effect of HCV on ConA-induced immunological hepatic injury and the influence of HCV on hepatic NK cell activation in the liver after ConA administration. An immunocompetent HCV mouse model that encodes the entire viral polyprotein in a liver-specific manner based on hydrodynamic injection and φC31o integrase was used to study the role of hepatic NK cells. Interestingly, the frequency of hepatic NK cells was reduced in HCV mice, whereas the levels of other intrahepatic lymphocytes remained unaltered...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28865999/a-novel-vaccine-targeting-glypican-3-as-a-treatment-for-hepatocellular-carcinoma
#16
Qunfeng Wu, Liya Pi, Thu Le Trinh, Chaohui Zuo, Man Xia, Yu Jiao, Zhouhua Hou, Sung Jo, William Puszyk, Kien Pham, David R Nelson, Keith Robertson, David Ostrov, Pranela Rameshwar, Chang Qing Xia, Chen Liu
Hepatocellular carcinoma (HCC) has a high morbidity and mortality rate worldwide, with limited treatment options. Glypican-3 (GPC3) is a glycosylphosphatidylinositol-anchored glycoprotein that is overexpressed in most HCC tissues but not in normal tissues. GPC3-targeting antibody therapy shows limited response in a clinical trial due to the lack of a tumor-specific cytotoxic T lymphocyte (CTL) response. Here, in C57/B6 mice, we demonstrated that intravenous infusion of GPC3-coupled lymphocytes (LC/GPC3(+)) elicited robust GPC3-specific antibody and CTL responses, which effectively restricted proliferation and lysed cultured-HCC cells...
October 4, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28863861/neuromyelitis-optica-atypical-hemophagocytic-lymphohistiocytosis-and-heterozygous-perforin-a91v-mutation
#17
Boaz Palterer, Francesca Brugnolo, Elena Sieni, Alessandro Barilaro, Paola Parronchi
Neuromyelitis optica is an autoimmune demyelinating inflammatory disease characterized by optic neuritis and myelitis with anti-aquaporin 4 antibodies. Hemophagocytic lymphohistiocytosis is a severe systemic inflammatory syndrome that can present in a genetic primary form or secondarily to infective, neoplastic or autoimmune diseases. Our case discusses the first reported case of atypical late-onset hemophagocytic lymphohistiocytosis in a patient with neuromyelitis optica, with multiple triggering factors and carrying the common A91V hypomorphic perforin mutation, that blurs the distinction between primary and secondary forms...
October 15, 2017: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/28862949/high-perforin-positive-cardiac-cell-infiltration-and-male-sex-predict-adverse-long-term-mortality-in-patients-with-inflammatory-cardiomyopathy
#18
Felicitas Escher, Uwe Kühl, Dirk Lassner, Andrea Stroux, Ulrich Gross, Dirk Westermann, Burkert Pieske, Wolfgang Poller, Heinz-Peter Schultheiss
BACKGROUND: The authors analyzed the effects of perforin-dependent infiltration on long-term mortality in patients with inflammatory cardiomyopathy (CMi). We previously demonstrated that left ventricular function deteriorates and progresses to substantial cardiac dysfunction in patients with perforin-positive cardiac cell infiltration. METHODS AND RESULTS: Between 2003 and 2013, 2389 consecutive patients with clinically suspected CMi who underwent endomyocardial biopsies were enrolled...
August 18, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28860075/cpg-odn-1668-induce-innate-and-adaptive-immune-responses-in-rock-bream-oplegnathus-fasciatus-against-rock-bream-iridovirus-rbiv-infection
#19
Myung-Hwa Jung, Sung-Ju Jung
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream in Korea. CpG ODN 1668 showed promise as immunoprotective agents against RBIV infection in rock bream. In this study, we assessed innate/adaptive-related gene expression patterns in RBIV-infected rock bream with and without CpG ODN 1668 administration to determine important immune defense related factors that may affect fish survival. In the CpG ODN 1668+virus-injected group, virus copies were more than 7.4- to 790591-fold lower than in the virus-injected group at 4 d (8...
August 30, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28858434/cd4-cd28-null-t-cells-are-not-alloreactive-unless-stimulated-by-interleukin-15
#20
B Dedeoglu, N H R Litjens, M Klepper, R Kraaijeveld, W Verschoor, C C Baan, M G H Betjes
Proinflammatory, cytotoxic CD4(+) CD28(null) T cells can be substantially expanded in patients with end-stage renal disease. These cells have been associated with the risk for rejection, but their alloreactive potential is unknown. CD4(+) CD28(null) T cells were stimulated with HLA-mismatched antigen presenting cells in the absence/presence of exogenous cytokines. Alloreactive potential was evaluated based on proliferation, degranulation, cytotoxicity, and cytokine production. Further, their suppressive capacity was assessed by measuring inhibition of proliferating alloreactive CD28(+) T cells...
August 31, 2017: American Journal of Transplantation
keyword
keyword
68638
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"