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https://www.readbyqxmd.com/read/28445856/procyanidin-a-kind-of-biological-flavonoid-induces-protective-anti-tumor-immunity-and-protects-mice-from-lethal-b16f10-challenge
#1
Lina Zhang, Shuang Wang, Zeyuan Liu, Li Zhang, Shanzheng Wang, Bin Wang
Recently, increasing evidences show that procyanidin (PC) modulate immune responses in human. To evaluate adjuvant effects of PC on vaccine immune modulation and anti-tumor activity, we formulated PC with B16F10 tumor antigen as tumor vaccine to immune C57BL/6 mice and used intramuscular injection before challenge with tumor B16F10 cells. Our results revealed that PC enhanced T cell-mediated immune responses both in vitro and in vivo. Moreover, the B16F10 tumor vaccine induced some degree of anti-tumor effects as evaluated by the inhibition of tumor growth and the prolongation of survival...
April 23, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28441851/-quantities-and-function-of-nk-cells-in-patients-with-immune-thrombocytopenia
#2
Y J Zhang, W Qu, H Liu, Y H Wang, C Y Liu, L J Li, H Q Wang, R Fu, L M Xing, Z H Shao
Objective: To investigate natural killer (NK) cell quantities and function in patients with immune thrombocytopenia (ITP) . Methods: A total of 66 ITP patients (34 newly diagnosed and 32 in complete remission) were collected from September 2015 to May 2016 in Tianjin Medical University General Hospital, and 30 healthy volunteers were recruited as controls. The percentages of NK cells and their subsets in peripheral blood, the expression of activating receptor (NKp44), inhibitory receptor (NKG2A) and CD16, perforin and granzyme β were detected by flow cytometry...
April 25, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28438315/tgf-%C3%AE-1-improving-abnormal-pregnancy-outcomes-induced-by-toxoplasma-gondii-infection-regulating-nkg2d-dap10-and-killer-subset-of-decidual-nk-cells
#3
Xiaoyan Xu, Jian Zhang, Shaowei Zhan, Zhidan Li, Xianbing Liu, Haixia Zhang, Yuzhu Jiang, Xuemei Hu
Our current aim was to investigate whether injection of TGF-β1 played an important role in improving abnormal pregnancy outcomes with T. gondii infection and how the TGF-β1 regulated. Results showed that TGF-β1 exhibited improved pregnancy outcomes induced by T. gondii infection. dNK cytotoxicity was increased with T. gondii infection while decreased with TGF-β1 treatment. dNK cytotoxicity related NKG2D/DAP10 expression, perforin, granzyme, IFN-γ and killer subsets were all increased with T. gondii infection while decreased after TGF-β1 treatment...
April 18, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28431214/interferon-%C3%AE-released-by-activated-cd8-t-lymphocytes-impairs-the-calcium-resorption-potential-of-osteoclasts-in-calcified-human-aortic-valves
#4
Edit Nagy, Yang Lei, Eduardo Martínez-Martínez, Simon C Body, Florian Schlotter, Michael Creager, Alexander Assmann, Kamal Khabbaz, Peter Libby, Göran K Hansson, Elena Aikawa
Calcium content in patients with calcific aortic valve disease (CAVD) correlates with the severity of stenosis. In CAVD, activated T lymphocytes localize with osteoclast regions; however, the functional consequences of this association remain unknown. We hypothesized that CD8(+) T cells modulate calcification in CAVD. Explanted CAVD valves (n = 52) dissected into noncalcified and calcified portions were subjected to mRNA extraction, real-time quantitative PCR, enzyme-linked immunosorbent assay, and immunohistochemical analyses...
April 18, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28429258/hormonal-regulation-of-nk-cell-cytotoxic-activity
#5
S V Shirshev, I V Nekrasova, O L Gorbunova, E G Orlova
The effects of chorionic gonadotropin, estriol (E3), leptin, ghrelin, and kisspeptin on the intracellular expression of perforin, granzyme A, and granzyme B was studied in separated NK cells. All studied hormones except E3 are could modulate the expression of cytotoxic enzymes in NK cells by suppression of the expression of the most active proapoptotic agents, resulting in increased expression of granzyme A, which is typical of the decidual subpopulation of these lymphocytes.
January 2017: Doklady Biological Sciences: Proceedings of the Academy of Sciences of the USSR, Biological Sciences Sections
https://www.readbyqxmd.com/read/28428612/granzyme-k-deficient-mice-show-no-evidence-of-impaired-anti-viral-immunity
#6
Lars T Joeckel, Cody C Allison, Mark Pellegrini, Catherina H Bird, Phillip Ian Bird
The biological role of granzyme K, a serine protease of cytotoxic T lymphocytes (CTL), is controversial. It has been reported to induce perforin-mediated cell death in vitro, but is also reported to be non-cytotoxic and to operate in inflammatory processes. To elucidate the biological role of this protease we have deleted the granzyme K gene in mice (mutant allele: Gzmk(tm1.1Pib); MGI:5636646). Gzmk(-/-) mice are healthy, anatomically normal, fecund, and show normal hematopoietic development. Gzmk(-/-) mice readily recover from Lymphocytic choriomeningitis virus and mouse pox Ectromelia virus infection...
April 21, 2017: Immunology and Cell Biology
https://www.readbyqxmd.com/read/28422754/mpeg1-perforin-2-mutations-in-human-pulmonary-nontuberculous-mycobacterial-infections
#7
Ryan M McCormack, Eva P Szymanski, Amy P Hsu, Elena Perez, Kenneth N Olivier, Eva Fisher, E Brook Goodhew, Eckhard R Podack, Steven M Holland
Perforin-2 is a highly conserved pore-forming protein encoded by macrophage expressed gene 1 (MPEG1). A number of studies have shown that Perforin-2-deficient mice are unable to survive following a bacterial challenge that is nonlethal in WT mice. There is also recent evidence that Mpeg1+/- heterozygous mice display an intermediate killing ability compared with Mpeg1 WT and Mpeg1-/- mice. Despite these in vivo findings, to date, no perforin-2 deficiencies have been associated with human disease. Here, we report four patients with persistent nontuberculous mycobacterial infection who had heterozygous MPEG1 mutations...
April 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28410483/contributions-of-cd8-t-cells-to-the-pathogenesis-of-mouse-adenovirus-type-1-respiratory-infection
#8
Caitlyn T Molloy, Jennifer S Andonian, Harrison M Seltzer, Megan C Procario, Michael E Watson, Jason B Weinberg
CD8 T cells are key components of the immune response to viruses, but their roles in the pathogenesis of adenovirus respiratory infection have not been characterized. We used mouse adenovirus type 1 (MAV-1) to define CD8 T cell contributions to the pathogenesis of adenovirus respiratory infection. CD8 T cell deficiency in β2m(-/-) mice had no effect on peak viral replication in lungs, but clearance of virus was delayed in β2m(-/-) mice. Virus-induced weight loss and increases in bronchoalveolar lavage fluid total protein, IFN-γ, TNF-α, IL-10, CCL2, and CCL5 concentrations were less in β2m(-/-) mice than in controls...
April 11, 2017: Virology
https://www.readbyqxmd.com/read/28408386/increased-pd-1-and-tim-3-tils-during-cetuximab-therapy-inversely-correlates-with-response-in-head-and-neck-cancer-patients
#9
Hyun-Bae Jie, Raghvendra M Srivastava, Athanassios Argiris, Julie E Bauman, Lawrence P Kane, Robert L Ferris
Despite emerging appreciation for the important role of immune checkpoint receptors in regulating the effector functions of T cells, it is unknown whether their expression is involved in determining the clinical outcome in response to cetuximab therapy. We examined the expression patterns of immune checkpoint receptors (including PD-1, CTLA-4, and TIM-3) and cytolytic molecules (including granzyme B and perforin) of CD8+ tumorinfiltrating lymphocytes (TILs) and compared them to those of peripheral blood T lymphocytes (PBLs) in patients with head and neck cancer (HNSCC) during cetuximab therapy...
April 13, 2017: Cancer Immunology Research
https://www.readbyqxmd.com/read/28402512/defective-trophoblast-invasion-underlies-fetal-growth-restriction-and-pre-eclampsia-like-symptoms-in-the-stroke-prone-spontaneously-hypertensive-rat
#10
G Barrientos, M Pussetto, M Rose, A C Staff, S M Blois, J E Toblli
STUDY QUESTION: What is the impact of chronic hypertension on placental development, fetal growth and maternal outcome in the stroke-prone spontaneously hypertensive rat (SHRSP)? SUMMARY ANSWER: SHRSP showed an impaired remodeling of the spiral arteries and abnormal pattern of trophoblast invasion during placentation, which were associated with subsequent maternal glomerular injury and increased baseline hypertension as well as placental insufficiency and asymmetric fetal growth restriction...
April 11, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28392788/the-transcription-factor-hobit-identifies-human-cytotoxic-cd4-t-cells
#11
Anna E Oja, Felipe A Vieira Braga, Ester B M Remmerswaal, Natasja A M Kragten, Kirsten M L Hertoghs, Jianmin Zuo, Paul A Moss, René A W van Lier, Klaas P J M van Gisbergen, Pleun Hombrink
The T cell lineage is commonly divided into CD4-expressing helper T cells that polarize immune responses through cytokine secretion and CD8-expressing cytotoxic T cells that eliminate infected target cells by virtue of the release of cytotoxic molecules. Recently, a population of CD4(+) T cells that conforms to the phenotype of cytotoxic CD8(+) T cells has received increased recognition. These cytotoxic CD4(+) T cells display constitutive expression of granzyme B and perforin at the protein level and mediate HLA class II-dependent killing of target cells...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28390966/il-17-and-ifn-%C3%AE-producing-nk-and-%C3%AE-%C3%AE-t-cells-are-preferentially-expanded-in-synovial-fluid-of-patients-with-reactive-arthritis-and-undifferentiated-spondyloarthritis
#12
Abhra Chandra Chowdhury, Smriti Chaurasia, Shravan Kumar Mishra, Amita Aggarwal, Ramnath Misra
IL-17/1L-23 axis is important in the pathogenesis of spondyloarthropathy. Besides Th17 cells, innate cells also produce IL-17. We studied the frequencies of natural killer (NK) (total, CD56(bright), CD56(dim), perforin+and granzyme+), NK-T, and γδ-T cells. In addition, IFN-γ, IL-17 producing NK and γδ-T cells were also studied. Peripheral blood (PB) from 40 patients (38 males) and paired synovial fluid (SF) from 34 patients with ReA/uSpA and PB from 11 healthy controls (HC) were studied. Frequency of γδ-T cells was decreased (p<0...
April 5, 2017: Clinical Immunology: the Official Journal of the Clinical Immunology Society
https://www.readbyqxmd.com/read/28385330/a-similar-local-immune-and-oxidative-stress-phenotype-in-vitiligo-and-halo-nevus
#13
Yuqi Yang, Shuli Li, Guannan Zhu, Qian Zhang, Gang Wang, Tianwen Gao, Chunying Li, Lin Wang, Zhe Jian
BACKGROUND: Vitiligo and halo nevus are two common T-cell-mediated skin disorders. Although autoimmunity has been suggested to be involved in both diseases, the relationship between vitiligo and halo nevus is not fully understood. OBJECTIVE: The aim of the current study was to investigate whether vitiligo and halo nevus share the same immunological and oxidative stress response. METHODS: Infiltrations of T cells, and expressions of chemokine receptors (CXCR3, CCR4, CCR5) and cytotoxic markers (Granzyme B, Perforin) in the lesions of vitiligo and halo nevus were examined by immunohistochemistry...
March 19, 2017: Journal of Dermatological Science
https://www.readbyqxmd.com/read/28378637/primary-tr1-cells-from-metastatic-melanoma-eliminate-tumor-promoting-macrophages-through-granzyme-b-and-perforin-dependent-mechanisms
#14
Hongxia Yan, Ping Zhang, Xue Kong, Xianglian Hou, Li Zhao, Tianhang Li, Xiaozhou Yuan, Hongjun Fu
In malignant melanoma, tumor-associated macrophages play multiple roles in promoting tumor growth, such as inducing the transformation of melanocytes under ultraviolet irradiation, increasing angiogenesis in melanomas, and suppressing antitumor immunity. Because granzyme B- and perforin-expressing Tr1 cells could specifically eliminate antigen-presenting cells of myeloid origin, we examined whether Tr1 cells in melanoma could eliminate tumor-promoting macrophages and how the interaction between Tr1 cells and macrophages could affect the growth of melanoma cells...
April 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28377769/antibody-dependent-cell-mediated-cytotoxicity-epitopes-on-the-hemagglutinin-head-region-of-pandemic-h1n1-influenza-virus-play-detrimental-roles-in-h1n1-infected-mice
#15
Zi-Wei Ye, Shuofeng Yuan, Kwok-Man Poon, Lei Wen, Dong Yang, Zehua Sun, Cun Li, Meng Hu, Huiping Shuai, Jie Zhou, Mei-Yun Zhang, Bo-Jian Zheng, Hin Chu, Kwok-Yung Yuen
Engaging the antibody-dependent cell-mediated cytotoxicity (ADCC) for killing of virus-infected cells and secretion of antiviral cytokines and chemokines was incorporated as one of the important features in the design of universal influenza vaccines. However, investigation of the ADCC epitopes on the highly immunogenic influenza hemagglutinin (HA) head region has been rarely reported. In this study, we determined the ADCC and antiviral activities of two putative ADCC epitopes, designated E1 and E2, on the HA head of a pandemic H1N1 influenza virus in vitro and in a lethal mouse model...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28366698/steroid-free-immunosuppression-is-associated-with-enhanced-th1-transcripts-in-kidney-transplantation
#16
Petra Hruba, Irena Tycova, Eva Krepsova, Eva Girmanova, Alena Sekerkova, Janka Slatinska, Ilja Striz, Eva Honsova, Ondrej Viklicky
BACKGROUND: Steroid avoidance in immunosuppression in kidney transplantation offers several metabolic advantages, however it is associated with higher early acute rejection rate. Cellular and molecular mechanisms of this phenomenon remain poorly understood. METHODS: In this single center observational study, low-risk kidney transplant recipients randomized into large multicenter prospective ADVANCE trial with steroid avoidance/early withdrawal and center standard of care treated patients were monitored for 12months...
March 30, 2017: Transplant Immunology
https://www.readbyqxmd.com/read/28364502/altered-cd8-t-cell-lymphocyte-function-and-tc1-cell-stemness-contribute-to-enhanced-malignant-tumor-properties-in-murine-models-of-sleep-apnea
#17
Mahzad Akbarpour, Abdelnaby Khalyfa, Zhuanghong Qiao, Alex Gileles-Hillel, Isaac Almendros, Ramon Farré, David Gozal
Study Objective: The presence of obstructive sleep apnea (OSA) in patients with cancer appears to be accompanied by poorer outcomes. However, the mechanisms underlying such association are unknown. Tumor infiltrating lymphocytes (TILs), including CD8+ T cells, function as cytotoxic T lymphocytes (CTLs) and mount immune responses to cancer by the release of cytolytic enzymes, including granzyme B (GzmB), perforin (Prf), and cytokines such as interferon (IFN)-γ. Methods: Using established in vivo mouse models, we investigated CD8+ T cells and cancer stem cells (CSCs) in intermittent hypoxia (IH) and sleep fragmentation (SF) in the context of tumor environment...
February 1, 2017: Sleep
https://www.readbyqxmd.com/read/28355563/cell-traversal-activity-is-important-for-plasmodium-falciparum-liver-infection-in-humanized-mice
#18
Annie S P Yang, Matthew T O'Neill, Charlie Jennison, Sash Lopaticki, Cody C Allison, Jennifer S Armistead, Sara M Erickson, Kelly L Rogers, Andrew M Ellisdon, James C Whisstock, Rebecca E Tweedell, Rhoel R Dinglasan, Donna N Douglas, Norman M Kneteman, Justin A Boddey
Malaria sporozoites are deposited into the skin by mosquitoes and infect hepatocytes. The molecular basis of how Plasmodium falciparum sporozoites migrate through host cells is poorly understood, and direct evidence of its importance in vivo is lacking. Here, we generated traversal-deficient sporozoites by genetic disruption of sporozoite microneme protein essential for cell traversal (PfSPECT) or perforin-like protein 1 (PfPLP1). Loss of either gene did not affect P. falciparum growth in erythrocytes, in contrast with a previous report that PfPLP1 is essential for merozoite egress...
March 28, 2017: Cell Reports
https://www.readbyqxmd.com/read/28355549/a-sigmoid-functional-response-emerges-when-cytotoxic-t-lymphocytes-start-killing-fresh-target%C3%A2-cells
#19
Saikrishna Gadhamsetty, Athanasius F M Marée, Joost B Beltman, Rob J de Boer
Cytotoxic T lymphocyte (CTL)-mediated killing involves the formation of a synapse with a target cell, followed by delivery of perforin and granzymes. Previously, we derived a general functional response for CTL killing while considering that CTLs form stable synapses (i.e., single-stage) and that the number of conjugates remains at steady state. However, the killing of target cells sometimes requires multiple engagements (i.e., multistage). To study how multistage killing and a lack of steady state influence the functional response, we here analyze a set of differential equations as well as simulations employing the cellular Potts model, in both cases describing CTLs that kill target cells...
March 28, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28345650/rational-combination-of-oncolytic-vaccinia-virus-and-pd-l1-blockade-works-synergistically-to-enhance-therapeutic-efficacy
#20
Zuqiang Liu, Roshni Ravindranathan, Pawel Kalinski, Z Sheng Guo, David L Bartlett
Both anti-PD1/PD-L1 therapy and oncolytic virotherapy have demonstrated promise, yet have exhibited efficacy in only a small fraction of cancer patients. Here we hypothesized that an oncolytic poxvirus would attract T cells into the tumour, and induce PD-L1 expression in cancer and immune cells, leading to more susceptible targets for anti-PD-L1 immunotherapy. Our results demonstrate in colon and ovarian cancer models that an oncolytic vaccinia virus attracts effector T cells and induces PD-L1 expression on both cancer and immune cells in the tumour...
March 27, 2017: Nature Communications
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