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https://www.readbyqxmd.com/read/28735417/design-and-properties-of-an-immobilization-enzyme-system-for-inulin-conversion
#1
Hua Hang, Changbao Wang, Yiqun Cheng, Ning Li, Liuli Song
A commercial inulinase could convert inulin into fructose, which was optimized to be entrapped in the calcium alginate-gelatin beads with the immobilization yield of 86% for free inulinase activities. The optimum pH values and temperatures were 4.5 and 40 °C for the free enzyme and 5.0-5.5 and 45-50 °C for the immobilized enzyme. The kinetic parameters of V max and K m were 5.24 μmol/min and 57.6 mg/mL for the free inulinase and 4.32 μmol/min and 65.8 mg/mL for the immobilized inulinase, respectively...
July 22, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28735377/study-of-the-correlation-between-fengycin-promoter-expression-and-its-production-by-bacillus-subtilis-under-different-culture-conditions-and-the-impact-on-surfactin-production
#2
Yazen Yaseen, Frédérique Gancel, Max Béchet, Djamel Drider, Philippe Jacques
This work aimed to rely expression of the fengycin promoter to fengycin production under different culture conditions. To this end, Bacillus subtilis BBG208, derived from BBG21, which is a fengycin overproducing strain carrying the green fluorescent protein (GFP) under the control of fengycin promoter, was used to assess the effects of different carbon and nitrogen sources on surfactin and fengycin production and the fengycin promoter expression. The data showed that some carbon sources oriented synthesis of one family of lipopeptides, while most of the nitrogen sources allowed high co-production of fengycin and surfactin...
July 22, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28735289/prolonged-fever-after-st-segment-elevation-myocardial-infarction-and-long-term-cardiac-outcomes
#3
Chika Kawashima, Yasushi Matsuzawa, Eiichi Akiyama, Masaaki Konishi, Hiroyuki Suzuki, Katsutaka Hashiba, Toshiaki Ebina, Masami Kosuge, Kiyoshi Hibi, Kengo Tsukahara, Noriaki Iwahashi, Nobuhiko Maejima, Kentaro Sakamaki, Satoshi Umemura, Kazuo Kimura, Kouichi Tamura
BACKGROUND: The biphasic inflammation after ST-segment elevation myocardial infarction (STEMI) plays an important role in myocardial healing and progression of systemic atherosclerosis. The purpose of this study is to investigate the impact of fever during the first and second phases of post-STEMI inflammation on long-term cardiac outcomes. METHODS AND RESULTS: A total of 550 patients with STEMI were enrolled in this study. Axillary body temperature (BT) was measured and maximum BTs were determined for the first (within 3 days: max-BT1-3d) and second (from 4 to 10 days after admission: max-BT4-10d) phases, respectively...
July 22, 2017: Journal of the American Heart Association
https://www.readbyqxmd.com/read/28734523/laparoscopic-choledochoduodenostomy
#4
Adolfo Cuendis-Velázquez, Mario E Trejo-Ávila, Enrique Rosales-Castañeda, Eduardo Cárdenas-Lailson, Martin E Rojano-Rodríguez, Sujey Romero-Loera, Carlos A Sanjuan-Martínez, Mucio Moreno-Portillo
INTRODUCTION: Today's options for biliary bypass procedures, for difficult choledocholithiasis, range from open surgery to laparo-endoscopic hybrid procedures. The aim of this study was to analyze the outcomes of patients with difficult choledocholithiasis treated with laparoscopic choledochoduodenostomy. METHODS: We performed a prospective observational study from March 2011 to June 2016. We included patients with difficult common bile duct stones (recurrent or unresolved by ERCP) in which a biliary bypass procedure was required...
July 19, 2017: Cirugía Española
https://www.readbyqxmd.com/read/28734264/a-small-scale-portable-method-for-extracting-microplastics-from-marine-sediments
#5
Rachel L Coppock, Matthew Cole, Penelope K Lindeque, Ana M Queirós, Tamara S Galloway
Microplastics (plastic particles, 0.1 μm-5 mm in size) are widespread marine pollutants, accumulating in benthic sediments and shorelines the world over. To gain a clearer understanding of microplastic availability to marine life, and the risks they pose to the health of benthic communities, ecological processes and food security, it is important to obtain accurate measures of microplastic abundance in marine sediments. To date, methods for extracting microplastics from marine sediments have been disadvantaged by complexity, expense, low extraction efficiencies and incompatibility with very fine sediments...
July 19, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28734213/enhanced-cadmium-phytoremediation-of-glycine-max-l-through-bioaugmentation-of-cadmium-resistant-bacteria-assisted-by-biostimulation
#6
Pongsarun Rojjanateeranaj, Chirawee Sangthong, Benjaphorn Prapagdee
This study examined the potential of three strains of cadmium-resistant bacteria, including Micrococcus sp., Pseudomonas sp. and Arthrobacter sp., to promote root elongation of Glycine max L. seedlings, soil cadmium solubility and cadmium phytoremediation in G. max L. planted in soil highly polluted with cadmium with and without nutrient biostimulation. Micrococcus sp. promoted root length in G. max L. seedlings under toxic cadmium conditions. Soil inoculation with Arthrobacter sp. increased the bioavailable fraction of soil cadmium, particularly in soil amended with a C:N ratio of 20:1...
July 17, 2017: Chemosphere
https://www.readbyqxmd.com/read/28733938/molecular-cloning-heterologous-expression-and-functional-characterization-of-a-cellulolytic-enzyme-cel-prii-from-buffalo-rumen-metagenome
#7
Ravi K Shah, Amrutlal K Patel, Deepti M Davla, Ishan K Parikh, Ramalingam B Subramanian, Kamlesh C Patel, Subhash J Jakhesara, Chaitanya G Joshi
A cellulase encoding gene, Cel PRII, was identified from Mehsani buffalo rumen metagenome, and cloned and expressed in Escherichia coli BL21(DE3)pLysS. The 1170 bp full length gene encodes a 389 residue polypeptide (Cel PRII) containing a catalytic domain belonging to glycosyl hydrolase (GH) 5 family. The fusion protein consisting of the Cel PRII, thioredoxin tag and 6x Histidine tag with predicted molecular weight of 63 kDa when recovered from inclusion bodies under denaturing conditions, exhibited cellulolytic activity against carboxymethyl cellulose (CMC)...
August 2017: 3 Biotech
https://www.readbyqxmd.com/read/28733828/thermosensitive-in-situ-gel-based-on-solid-dispersion-for-rectal-delivery-of-ibuprofen
#8
Yangdan Liu, Xin Wang, Youping Liu, Xin Di
The objective of this study was to develop a thermosensitive in situ gel based on solid dispersions (SDs) for rectal delivery of ibuprofen (IBU). Thermosensitive (poloxamer 407) and mucoadhesive (hydroxypropylmethyl cellulose E5 and sodium alginate) polymers were used to prepare the in situ gel and the sol-gel transition temperature (T sol-gel) and gel strength were optimized. The in vitro release performance and in vivo pharmacokinetic properties of the in situ gel after their rectal administration to rabbits were investigated...
July 21, 2017: AAPS PharmSciTech
https://www.readbyqxmd.com/read/28733823/bioaccumulation-kinetics-of-arsenite-and-arsenate-in-dunaliella-salina-under-different-phosphate-regimes
#9
Ya Wang, Chunhua Zhang, Yanheng Zheng, Ying Ge
Dunaliella salina is a potential candidate for the phycoremediation of saline water contaminated with arsenic (As) due to its strong tolerance of salt and this toxic metalloid. However, the efficiency of As removal by this microalga varies under different phosphate regimes and the underlying mechanisms remain unresolved. Therefore, more detailed studies are needed to optimize As remediation using D. salina. Here, we investigated the dynamic processes of arsenite (As(III)) and arsenate (As(V)) uptake, transformation, and excretion by D...
July 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28733813/individual-and-combined-effects-of-cannabis-and-tobacco-on-drug-reward-processing-in-non-dependent-users
#10
Chandni Hindocha, Will Lawn, Tom P Freeman, H Valerie Curran
RATIONALE: Cannabis and tobacco are often smoked simultaneously in joints, and this practice may increase the risks of developing tobacco and/or cannabis use disorders. Currently, there is no human experimental research on how these drugs interact on addiction-related measures. OBJECTIVES: This study aimed to investigate how cannabis and tobacco, each alone and combined together in joints, affected individuals' demand for cannabis puffs and cigarettes, explicit liking of drug and non-drug-related stimuli and craving...
July 22, 2017: Psychopharmacology
https://www.readbyqxmd.com/read/28733455/e3-ubiquitin-ligase-nedd4-induces-endocytosis-and-lysosomal-sorting-of-connexin43-to-promote-loss-of-gap-junctions
#11
Max Z Totland, Christian H Bergsland, Tone A Fykerud, Lars M Knudsen, Nikoline L Rasmussen, Peter W Eide, Zeremariam Yohannes, Vigdis Sørensen, Andreas Brech, Ragnhild A Lothe, Edward Leithe
Intercellular communication via gap junctions has an important role in controlling cell growth and in maintaining tissue homeostasis. Connexin43 is the most abundantly expressed gap junction channel protein in humans and acts as a tumor suppressor in multiple tissue types. Connexin43 is often dysregulated at the post-translational level during cancer development, resulting in loss of gap junctions. However, the molecular basis underlying the aberrant regulation of connexin43 in cancer cells has remained elusive...
July 21, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28732298/stomatal-and-non-stomatal-factors-regulated-the-photosynthesis-of-soybean-seedlings-in-the-present-of-exogenous-bisphenol-a
#12
Liya Jiao, Lihong Wang, Qing Zhou, Xiaohua Huang
Bisphenol A (BPA) is an emerging environmental endocrine disruptor that has toxic effects on plants growth. Photosynthesis supplies the substances and energy required for plant growth, and regulated by stomatal and non-stomatal factors. Therefore, in this study, to reveal how BPA affects photosynthesis in soybean seedlings (Glycine max L.) from the perspective of stomatal and non-stomatal factors, the stomatal factors (stomatal conductance and behaviours) and non-stomatal factors (Hill reaction, apparent quantum efficiency, Rubisco activity, carboxylation efficiency, the maximum Rubisco carboxylation velocity, ribulose-1,5-bisphospate regeneration capacities mediated by maximum electron transport rates, and triose phosphate utilization rate) were investigated using a portable photosynthesis system...
July 18, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28731683/synthesis-and-optical-properties-of-cu6e6-snph-2-pph2et-6-e-s-se-te-cluster-molecules
#13
Andreas Eichhöfer, Michael Kühn, Sergei Lebedkin, Max Kehry, Manfred M Kappes, Florian Weigend
A homologous series of three copper-tin-chalcogenide cluster molecules [Cu6E6(SnPh)2(PPh2Et)6] (E = S, Se, Te) was synthesized by reactions of CuO(O)CCH3 and PhSnCl3 with E(SiMe3)2 in the presence of PPh2Et. The cluster cage structures are similar, with slight differences in the bridging modes of the respective chalcogenide ligands E. The onset of the optical absorption displays a significant decrease of ca. 1.1 eV on going from sulfur to tellurium. The differences in bonding and electronic excitations can be rationalized by DFT and TDDFT calculations...
July 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28731334/optical-spectra-and-magnetic-behavior-of-a-wide-range-of-europium-iii-oxo-compounds-analysis-of-the-ligand-field-effects
#14
Anna Bronova, Nils Kannengießer, Robert Glaum
The europium-oxygen interaction in nine different europium(III) oxo-compounds (including C-type Eu2O3) was investigated on the basis of powder reflectance spectra (near-IR/vis/UV) and temperature-dependent magnetic measurements. Computation of the transition energies and of the effective Bohr magneton numbers for Eu(3+) in the different ligand fields were performed within the framework of the angular overlap model (AOM) using the computer program BonnMag. These calculations show that all electronic transition energies in the optical spectra, the magnetic susceptibilities as well as their temperature dependence, are very well-accounted for by AOM...
July 21, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28731034/regulation-of-hepatic-glucose-metabolism-in-health-and-disease
#15
REVIEW
Max C Petersen, Daniel F Vatner, Gerald I Shulman
The liver is crucial for the maintenance of normal glucose homeostasis - it produces glucose during fasting and stores glucose postprandially. However, these hepatic processes are dysregulated in type 1 and type 2 diabetes mellitus, and this imbalance contributes to hyperglycaemia in the fasted and postprandial states. Net hepatic glucose production is the summation of glucose fluxes from gluconeogenesis, glycogenolysis, glycogen synthesis, glycolysis and other pathways. In this Review, we discuss the in vivo regulation of these hepatic glucose fluxes...
July 21, 2017: Nature Reviews. Endocrinology
https://www.readbyqxmd.com/read/28730456/photofunctionalization-and-non-thermal-plasma-activation-of-titanium-surfaces
#16
Anders Henningsen, Ralf Smeets, Philip Hartjen, Oliver Heinrich, Roman Heuberger, Max Heiland, Clarissa Precht, Claudio Cacaci
OBJECTIVE: The aim of this study was to compare UV light and non-thermal plasma (NTP) treatment regarding the improvement of physical material characteristics and cell reaction on titanium surfaces in vitro after short-term functionalization. MATERIALS AND METHODS: Moderately rough (Ra 1.8-2.0 μm) sandblasted and acid-etched titanium disks were treated by UV light (0.05 mW/cm(2) at λ = 360 nm and 2 mW/cm(2) at λ = 250 nm) or by NTP (24 W, -0.5 mbar) of argon or oxygen for 12 min each...
July 20, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28730290/a-phase-i-study-to-investigate-the-metabolism-excretion-and-pharmacokinetics-of-14-c-fruquintinib-a-novel-oral-selective-vegfr-inhibitor-in-healthy-chinese-male-volunteers
#17
Sufeng Zhou, Feng Shao, Zhaoqiang Xu, Lu Wang, Ke Jin, Lijun Xie, Juan Chen, Yun Liu, Hongwen Zhang, Ning Ou
PURPOSE: Fruquintinib (HMPL-013) is a novel, potent, and highly selective tyrosine kinase inhibitor targeting the vascular endothelial growth factor receptors (1, 2 and 3). This study was conducted to investigate the metabolism, excretion, and pharmacokinetics of HMPL-013 after a single oral dose to healthy Chinese men. METHODS: Six subjects were administrated an oral suspension containing 5 mg of (14)C-labeled HMPL-013 (100 μCi) in a fasted state. Blood and excreta samples were collected at the designated time points or intervals for pharmacokinetics and radiometric analyses...
July 20, 2017: Cancer Chemotherapy and Pharmacology
https://www.readbyqxmd.com/read/28729931/validity-and-reliability-of-the-catwalk-system-as-a-static-and-dynamic-gait-analysis-tool-for-the-assessment-of-functional-nerve-recovery-in-small-animal-models
#18
Elisabeth A Kappos, Patricia K Sieber, Patricia E Engels, Alessio V Mariolo, Salvatore D'Arpa, Dirk J Schaefer, Daniel F Kalbermatten
INTRODUCTION: A range of behavioral testing paradigms have been developed for the research of central and peripheral nerve injuries with the help of small animal models. Following any nerve repair strategy, improved functional outcome may be the most important evidence of axon regeneration. A novel automated gait analysis system, the CatWalk(™), can measure dynamic as well as static gait patterns of small animals. Of most interest in detecting functional recovery are in particular dynamic gait parameters, coordination measures, and the intensity of the animals paw prints...
July 2017: Brain and Behavior
https://www.readbyqxmd.com/read/28729889/differential-methylation-at-mhc-in-cd4-t-cells-is-associated-with-multiple-sclerosis-independently-of-hla-drb1
#19
Vicki E Maltby, Rodney A Lea, Katherine A Sanders, Nicole White, Miles C Benton, Rodney J Scott, Jeannette Lechner-Scott
BACKGROUND: Although many genetic variants have been associated with multiple sclerosis (MS) risk, they do not explain all the disease risk and there remains uncertainty as to how these variants contribute to disease. DNA methylation is an epigenetic mechanism that can influence gene expression and has the potential to mediate the effects of environmental factors on MS. In a previous study, we found a differentially methylation region (DMR) at MHC HLA-DRB1 that was associated within relapsing-remitting MS (RRMS) patients in CD4(+) T cells...
2017: Clinical Epigenetics
https://www.readbyqxmd.com/read/28729876/genetic-architecture-of-flooding-tolerance-in-the-dry-bean-middle-american-diversity-panel
#20
Ali Soltani, Samira MafiMoghaddam, Katelynn Walter, Daniel Restrepo-Montoya, Sujan Mamidi, Stephan Schroder, Rian Lee, Phillip E McClean, Juan M Osorno
Flooding is a devastating abiotic stress that endangers crop production in the twenty-first century. Because of the severe susceptibility of common bean (Phaseolus vulgaris L.) to flooding, an understanding of the genetic architecture and physiological responses of this crop will set the stage for further improvement. However, challenging phenotyping methods hinder a large-scale genetic study of flooding tolerance in common bean and other economically important crops. A greenhouse phenotyping protocol was developed to evaluate the flooding conditions at early stages...
2017: Frontiers in Plant Science
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