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Kevin W George, Mitchell Thompson, Joonhoon Kim, Edward E K Baidoo, George Wang, Veronica Teixeira Benites, Christopher J Petzold, Leanne Jade G Chan, Suzan Yilmaz, Petri Turhanen, Paul D Adams, Jay D Keasling, Taek Soon Lee
Isopentenyl pyrophosphate (IPP) toxicity presents a challenge in engineered microbial systems since its formation is unavoidable in terpene biosynthesis. In this work, we develop an experimental platform to study IPP toxicity in isoprenol-producing Escherichia coli. We first characterize the physiological response to IPP accumulation, demonstrating that elevated IPP levels are linked to growth inhibition, reduced cell viability, and plasmid instability. We show that IPP toxicity selects for pathway "breakage", using proteomics to identify a reduction in phosphomevalonate kinase (PMK) as a probable recovery mechanism...
March 9, 2018: Metabolic Engineering
Faysal A Yafi, Robert Andrianne, Laith Alzweri, Jeffrey Brady, Michael Butcher, Daniel Chevalier, Kenneth J DeLay, Antoine Faix, Georgios Hatzichristodoulou, Wayne J G Hellstrom, Lawrence Jenkins, Tobias S Kohler, Daniar Osmanov, Sun Hung Park, M David Schwabb, Robert Valenzuela, Koenraad van Renterghem, Steven K Wilson
BACKGROUND: After radical prostatectomy (RP), erectile dysfunction, often necessitating the need for inflatable penile prosthesis (IPP) insertion, and urinary incontinence and climacturia can ensue. AIM: To assess the efficacy and safety of the mini-jupette, a mesh used to approximate the medial aspects of the 2 corporotomies at the time of IPP insertion, for the management of climacturia and urine leakage in patients with minimal incontinence. METHODS: We conducted a pilot multicenter study of patients with post-RP erectile dysfunction and climacturia and/or mild urinary incontinence (≤2 pads/day [ppd]) undergoing IPP insertion with concomitant placement of a mini-jupette graft...
March 6, 2018: Journal of Sexual Medicine
Xiaoduo Wen, Denggui Wen, Hui Zhang, Huifeng Zhang, Yi Yang
Rapid and noninvasive diagnosis on and differentiation between normal, central precocious puberty (CPP), and isolated precocious puberty (IPP) is imperative before a decision can be made with gonadotropin-releasing hormone (GnRH) agonist treatment. Our study aims to evaluate such a role by pelvic ultrasound.We consecutively enrolled 84 cases of IPP (59 with premature thelarche/ pubarche and 25 with premature menarche), 47 CPP, and 177 age-matched normal controls. The IPP and CPP were diagnosed by clinical examination and GnRH-stimulation test and confirmed by over 2 years' follow-up...
March 2018: Medicine (Baltimore)
Maribel Ramírez Torres, Roxana E Ruiz Valenzuela, Julián Esparza-Romero, Miriam T López Teros, Heliodoro Alemán-Mateo
BACKGROUND: Impaired physical performance (IPP) and physical disability (PD) are two serious public health problems in older adult populations worldwide. While studies show that changes in body composition are important risk factors for developing these conditions, there is little evidence that the fat-free mass (FFM) and fat mass (FM) indices (FFMI and FMI, respectively) are associated with IPP in older men and women. This study assessed the association among FFMI, FMI, and IPP using Short Physical Performance Battery (SPPB) in Mexican men and women aged over 60 years...
February 17, 2018: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
Haiyan Yang, Xuhai Yang, Zhe Zhang, Kunjuan Zhao
The current constellation of the BeiDou Navigation Satellite System (BDS) consists of five geostationary earth orbit (GEO) satellites, five inclined geosynchronous satellite orbit (IGSO) satellites, and four medium earth orbit (MEO) satellites. The advantage of using GEO satellites to monitor the ionosphereis the almost motionless ionospheric pierce point (IPP), which is analyzed in comparison with the MEO and IGSO satellites. The results from the analysis of the observations using eight tracking sites indicate that the ionospheric total electron content (TEC) sequence derived from each GEO satellite at their respective fixed IPPs is always continuous...
February 27, 2018: Sensors
Pietro Grande, Gabriele Antonini, Cristiano Cristini, Ettore De Berardinis, Antonio Gatto, Giovanni Di Lascio, Andrea Lemma, Giuseppe Gentile, Giovanni Battista Di Pierro
PURPOSE: To compare perioperative results, safety and efficacy profile in patients receiving inflatable penile prosthesis (IPP) via penoscrotal (PS) or minimally invasive infrapubic (MII) approach for erectile dysfunction. METHODS: A matched-pair analysis was performed including 42 patients undergoing IPP implantation via PS (n = 21) or MII (n = 21) between 2011 and 2016. Clinical and surgical data were prospectively collected. Patients' and partners' outcomes were assessed by the International Index of Erectile Function (IIEF), Erectile Dysfunction Inventory of Treatment Satisfaction (EDITS) and Quality of Life and Sexuality with Penile Prosthesis (QoLSPP) questionnaires...
February 28, 2018: World Journal of Urology
Caitlin McNair, Many Fung, Anna Taddio, Moshe Ipp, Steven Moss, Stephen Baker, Jonathan Tolkin, Malini Dave, Sharmeen Feerasta, Preeya Govan, Rebecca Pillai Riddell
No abstract text is available yet for this article.
July 2017: Paediatrics & Child Health
Jeffrey Loh-Doyle, Mukul B Patil, Hari Sawkar, Kevin Wayne, Stuart D Boyd
BACKGROUND: After radical cystoprostatectomy (RC), postoperative erectile dysfunction (ED) is a common consequence with multiple contributing etiologies. The inflatable penile prosthesis (IPP) offers patients a definitive treatment option when ED is refractory to medical therapies. Because of the hostile postoperative anatomy of these patients, a careful surgical approach is necessary for successful outcomes and to avoid adjacent organ injury. To date, there is no series describing the outcomes of 3-piece IPP placement in patients with urinary diversions...
February 13, 2018: Journal of Sexual Medicine
Pablo Ramón, Eduardo Velázquez, Adrián Escudero, Marcelino de la Cruz
We assessed the relative importance of dispersal limitation, environmental heterogeneity and their joint effects as determinants of the spatial patterns of 229 species in the moist tropical forest of Barro Colorado Island (Panama). We differentiated five types of species according to their dispersal syndrome; autochorous, anemochorous, and zoochorous species with small, medium-size and large fruits. We characterized the spatial patterns of each species and we checked whether they were best fitted by Inhomogeneous Poisson (IPP), Homogeneous Poisson cluster (HPCP) and Inhomogeneous Poisson cluster processes (IPCP) by means of the Akaike Information Criterion...
2018: PloS One
Bryndon J Oleson, Aaron Naatz, Sarah C Proudfoot, Chay Teng Yeo, John A Corbett
Nitric oxide is produced at micromolar levels by pancreatic β-cells during exposure to proinflammatory cytokines. While classically viewed as damaging, nitric oxide also activates pathways that promote β-cell survival. We have shown that nitric oxide, in a cell type selective manner, inhibits the DNA damage response (DDR) and, in doing so, protects β-cells from DNA damage-induced apoptosis. This study explores potential mechanisms by which nitric oxide inhibits DDR signaling. We show that inhibition of DDR signaling (measured by γH2AX formation and the phosphorylation of KAP1) is selective for nitric oxide, as other forms of reactive oxygen/nitrogen species do not impair DDR signaling...
February 14, 2018: Diabetes
Jiao Chen, Yaping Fan, Bomiao Cui, Xiaoying Li, Yu Yu, Yue Du, Qianming Chen, Yun Feng, Ping Zhang
γδT cells function in the regulation of T-cell activation in cancer and have been identified as a novel target for cancer immunotherapy. Activated γδT cells release a series of cytotoxic molecules-including granulysin, perforin, Fas/Fas ligand (Fas-L), and granzymes A and B-to kill target cells. Our previous research has shown that high mobility group nucleosomal-binding domain 2 (HMGN2), which is expressed at a high level in activated CD8T cells, is an antitumor effector molecule of CD8T cells. In the present study, we examined the expression and antitumor effects of HMGN2 in γδT cells...
February 2, 2018: Journal of Immunotherapy
L Topazio, C Perugia, C De Nunzio, G Gaziev, V Iacovelli, D Bianchi, G Vespasiani, E Finazzi Agrò
BACKGROUND: To investigate the efficacy of tamsulosin in patients with lower urinary tract symptoms (LUTS) and benign prostatic enlargement (BPE) with intravesical prostatic protrusion (IPP). Ultrasound measurement of the IPP has been previously described as an effective instrument for the evaluation of benign prostatic obstruction (BPO) and could help in clarifying the role of alpha-blockers in patients with (BPE). METHODS: Patients with BPE and LUTS were enrolled in this observational study...
February 2, 2018: BMC Urology
Xu Wang, Cynthia S Dowd
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), is a severe infectious disease in need of new chemotherapies especially for drug-resistant cases. To meet the urgent requirement of new TB drugs with novel modes of action, the TB research community has been validating numerous targets from several biosynthetic pathways. The methylerythritol phosphate (MEP) pathway is utilized by Mtb for the biosynthesis of isopentenyl pyrophosphate (IPP) and its isomer dimethylallyl pyrophosphate (DMAPP), the universal five-carbon building blocks of isoprenoids...
March 9, 2018: ACS Infectious Diseases
Han Jie Lee, Alvin Lee, Hong Hong Huang, Palaniappan Sundaram, Keong Tatt Foo
Objective: Despite high-grade intravesical prostatic protrusion (IPP) being closely related to bladder outlet obstruction (BOO), up to 21% of patients with low IPP remain obstructed. This study evaluates the characteristics and urodynamic findings of men with small prostates and low IPP. Methods: One hundred and fourteen men aged >50 years old with lower urinary tract symptoms (LUTS) were assessed with symptoms, uroflowmetry, serum prostate-specific antigen (PSA), transabdominal ultrasound measurement of prostate volume (PV), IPP and post-void residual urine (PVRU)...
October 2017: Asian Journal of Urology
Reshma Mangat, Henry S S Ho, Tricia L C Kuo
Lower urinary tract symptoms (LUTS) are common in males over the age of 40 years old and are likely to increase with an aging population. Currently urodynamic studies are the gold standard to determine the aetiology of voiding dysfunction and LUTS. However, due to its invasive nature, a great number of non-invasive ultrasound based investigations have been developed to assess patients with symptomatic LUTS. The clinical application of non-invasive tests could potentially stratify patients who would require more invasive investigations and allow more precise patient directed treatment...
January 2018: Asian Journal of Urology
X Wang, X Shu, Z Li, W Huo, L Zou, Y Tang, L Li
BACKGROUND: Skin imaging analysis, acting as a supplement to noninvasive bioengineering devices, has been widely used in medical cosmetology and cosmetic product evaluation. The main aim of this study is to assess the differences and correlations in measuring skin spots, wrinkles, vascular features, porphyrin, and pore between two commercially available image analysis software. MATERIALS AND METHODS: Seventy healthy women were included in the study. Before taking pictures, the dermatologist evaluated subjects' skin conditions...
January 27, 2018: Skin Research and Technology
G G Krivoborodov, N S Efremov, A D Bolotov
RELEVANCE: Benign prostatic hyperplasia (BPH) is one of the most common urological diseases among men. Despite the noticeable positive effects of pharmacotherapy on the quality of urination in BPH, the presence of an intravesical obstruction (IVO) leads to discontinuation of conservative treatment in favor of surgical interventions. One of the features of prostate enlargement is the degree of its intravesical growth (intravascular prostatic protrusion, IPP). According to some studies, IPP value of 10 mm or more is indicative of IVO in virtually all men...
December 2017: Urologii︠a︡
Juan-Pablo Caballero-Romeu, Jua-Antonio Galán-Llopis, Federico Soria, Esther Morcillo-Martín, Pablo Caballero-Pérez, Alejandro Garcia, Julia E De La Cruz-Conty, Jesús Romero-Maroto
PURPOSE: Ureteroscopy (URS) is related to complications, as fever or postoperative urinary sepsis, due to high intrapelvic pressure (IPP) during the procedure. Micro-ureteroscopy (m-URS) aims to reduce morbidity by miniaturizing the instrument. The objective of this study is to compare IPP and changes in renal haemodynamics, while performing m-URS vs. conventional URS. METHODS: A porcine model involving 14 female pigs was used in this experimental study. Two surgeons performed 7 URS (8/9...
January 25, 2018: World Journal of Urology
Marie McGovern, Perla Gisela Castaneda, Olga Pekar, Laura G Vallier, Erin J Cram, E Jane Albert Hubbard
DSL ligands activate the Notch receptor in many cellular contexts across metazoa to specify cell fate. In addition, Notch receptor activity is implicated in post-mitotic morphogenesis and neuronal function. In C. elegans, the DSL family ligand APX-1 is expressed in a subset of cells of the proximal gonad lineage, where it can act as a latent proliferation-promoting signal to maintain proximal germline tumors. Here we examine apx-1 in the proximal gonad and uncover a role in the maintenance of normal ovulation...
January 22, 2018: Developmental Biology
Teresa A O'Sullivan, Erin Sy
Objective. To design an experiential education curriculum that sequentially and deliberately prepares community pharmacy-bound graduates to practice at the level of the care provider and display the skills needed to be a pharmacist-in-charge. Methods. Semi-structured interviews were conducted with community pharmacy stakeholders. Transcriptions from the interviews were analyzed to identify common themes in needed community pharmacy training for core and elective advanced pharmacy practice experiences (APPEs)...
December 2017: American Journal of Pharmaceutical Education
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