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https://www.readbyqxmd.com/read/29030959/ipsc-derived-vascular-cell-spheroids-as-building-blocks-for-scaffold-free-biofabrication
#1
Leni Moldovan, April Barnard, Chang-Hyun Gil, Y Lin, Maria B Grant, Mervin C Yoder, Nutan Prasain, Nicanor I Moldovan
Recently a protocol was established to obtain large quantities of human induced pluripotent stem cells (iPSC)-derived endothelial progenitors, called endothelial colony forming cells (ECFC), and of candidate smooth-muscle forming cells (SMFC). Here, we tested their suitability for assembling in spheroids, and in larger 3D cell constructs. iPSC-derived ECFC and SMFC were labeled with tdTomato and eGFP, respectively. Spheroids were formed in ultra-low adhesive wells, and their dynamic proprieties were studied by time-lapse microscopy, or by confocal microscopy...
October 14, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/29024216/acrochordons-on-the-neck-a-remarkable-clinical-feature-of-tuberous-sclerosis-showing-different-patterns
#2
C Baykal
Sachs and Lipsker(1) recently published a few patients diagnosed with tuberous sclerosis (TS) presenting with multiple loosely arranged acrochordons (molluscum pendulum) located on the posterior neck showing a lace-like distribution and they proposed the term "molluscum pendulum necklace sign" for these typically located lesions. They detected acrochordons in five cases (17%) in a retrospectively analysed consecutive series of 29 TS patients, of whom four (three adults and one child) remarkably showed this typical pattern...
October 12, 2017: Journal of the European Academy of Dermatology and Venereology: JEADV
https://www.readbyqxmd.com/read/29021583/polydopamine-laced-biomimetic-material-stimulation-of-bone-marrow-derived-mesenchymal-stem-cells-to-promote-osteogenic-effects
#3
Dong Joon Lee, Yan-Ting Lee, Rui Zou, Renie Daniel, Ching-Chang Ko
A hydroxyapatite-collagen (HC) composite material can mimic composition and ultra-structures of natural bone and provide adequate bioactive material-tissue interactions. Incorporation of dopamine (DA) is one of keys in increasing the mechanical strength of the HC material to approaching that of cortical bone. In this study, the in vitro osteogenic effects of polydopamine-laced hydroxyapatite collagen calcium silicate (HCCS-PDA) were examined by culturing rat mesenchymal stem cells (rMSCs) on HCCS-PDA and HCCS coated plates...
October 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28970815/the-evolutionary-psychology-of-envy-and-jealousy
#4
Vilayanur S Ramachandran, Baland Jalal
The old dogma has always been that the most complex aspects of human emotions are driven by culture; Germans and English are thought to be straight-laced whereas Italians and Indians are effusive. Yet in the last two decades there has been a growing realization that even though culture plays a major role in the final expression of human nature, there must be a basic scaffolding specified by genes. While this is recognized to be true for simple emotions like anger, fear, and joy, the relevance of evolutionary arguments for more complex nuances of emotion have been inadequately explored...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28888327/vicarious-social-defeat-stress-induces-depression-related-outcomes-in-female-mice
#5
Sergio D Iñiguez, Francisco J Flores-Ramirez, Lace M Riggs, Jason B Alipio, Israel Garcia-Carachure, Mirella A Hernandez, David O Sanchez, Mary Kay Lobo, Peter A Serrano, Stephen H Braren, Samuel A Castillo
BACKGROUND: Stress is a prevailing risk factor for mood-related illnesses, wherein women represent the majority of those affected by major depression. Despite the growing literature suggesting that affective disorders can arise after a traumatic event is vicariously experienced, this relationship remains understudied in female subjects at the preclinical level. Thus, the objective of the current investigation was to examine whether exposure to emotional and/or psychological stress (ES) mediates depression-related outcomes in female mice...
July 29, 2017: Biological Psychiatry
https://www.readbyqxmd.com/read/28855957/discrimination-of-boron-tolerance-in-pisum-sativum-l-genotypes-using-a-rapid-high-throughput-hydroponic-screen-and-precociously-germinated-seed-grown-under-far-red-enriched-light
#6
Richard G Bennett, Federico M Ribalta, Maria Pazos-Navarro, Antonio Leonforte, Janine S Croser
BACKGROUND: Boron (B) tolerance has been identified as a key target for field pea improvement. Screening for B tolerance in the field is problematic due to variability in space and time, and robust B molecular markers are currently unavailable in field pea. There has been recent progress in developing protocols that can accelerate the life cycle of plants to enable rapid generation turnover in single seed descent breeding programs. A robust B screening protocol that can be fully integrated within an accelerated single seed descent system could lead to rapid identification and introgression of B tolerance into field pea genotypes...
2017: Plant Methods
https://www.readbyqxmd.com/read/28831932/-a-rare-cause-of-insufficiency-fractures
#7
M A W Hermans, P L A van Daele
A 41-year-old male with a history of cutaneous sarcoidosis presented with sudden onset pain in his left foot. An X-ray showed cystic lesions in the proximal phalanges of the foot with two insufficiency fractures. The lace-like pattern of these lesions is exemplary for osseous sarcoidosis.
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28802244/detoxification-of-diphenyl-ether-herbicide-lactofen-by-bacillus-sp-za-and-enantioselective-characteristics-of-an-esterase-gene-lace
#8
Jing Zhang, Luyao Lu, Feng Chen, Lingling Chen, Jingang Yin, Xing Huang
A bacterial strain Za capable of degrading diphenyl ether herbicide lactofen was isolated and identified as Bacillus sp. This strain could degrade 94.8% of 50mgL(-1) lactofen after 4days of inoculation in flasks. It was revealed that lactofen was initially hydrolyzed to desethyl lactofen, which was further transformed to acifluorfen, followed by the reduction of the nitro group to yield aminoacifluorfen. The phytotoxicity of the transformed product aminoacifluorfen to maize was decreased significantly compared with the lactofen...
August 3, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28790370/duplication-events-downstream-of-irx1-cause-north-carolina-macular-dystrophy-at-the-mcdr3-locus
#9
Valentina Cipriani, Raquel S Silva, Gavin Arno, Nikolas Pontikos, Ambreen Kalhoro, Sandra Valeina, Inna Inashkina, Mareta Audere, Katrina Rutka, Bernard Puech, Michel Michaelides, Veronica van Heyningen, Baiba Lace, Andrew R Webster, Anthony T Moore
Autosomal dominant North Carolina macular dystrophy (NCMD) is believed to represent a failure of macular development. The disorder has been linked to two loci, MCDR1 (chromosome 6q16) and MCDR3 (chromosome 5p15-p13). Recently, non-coding variants upstream of PRDM13 (MCDR1) and a duplication including IRX1 (MCDR3) have been identified. However, the underlying disease-causing mechanism remains uncertain. Through a combination of sequencing studies on eighteen NCMD families, we report two novel overlapping duplications at the MCDR3 locus, in a gene desert downstream of IRX1 and upstream of ADAMTS16...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28772129/the-effects-of-ankle-braces-and-taping-on-lower-extremity-running-kinematics-and-energy-expenditure-in-healthy-non-injured-adults
#10
Kaori Tamura, Kara N Radzak, Rachele E Vogelpohl, Bethany A Wisthoff, Yukiya Oba, Ronald K Hetzler, Christopher D Stickley
Ankle braces and taping are commonly used to prevent ankle sprains and allow return to play following injury, however, it is unclear how passive restriction of joint motion may effect running gait kinematics and energy expenditure during exercise. The purpose of this study was to determine the effect of different types of ankle supports on lower extremity kinematics and energy expenditure during continuous running. Thirteen healthy physically active adults ran at self-selected speed on the treadmill for 30min in four different ankle support conditions: semi-rigid hinged brace, lace-up brace, tape and control...
October 2017: Gait & Posture
https://www.readbyqxmd.com/read/28771704/parallel-environmental-factors-drive-variation-in-insect-density-and-plant-resistance-in-the-native-and-invaded-ranges
#11
Yuzu Sakata, Timothy P Craig, Joanne K Itami, Michimasa Yamasaki, Takayuki Ohgushi
Geographic variation in the traits of a species is shaped by variation in abiotic conditions, biotic interactions, and evolutionary history of its interactions with other species. We studied the geographic variation in the density of the lace bug, Corythucha marmorata, and the resistance of tall goldenrod Solidago altissima to the lace bug herbivory in their native range in the United States and invaded range in Japan. We conducted field surveys and reciprocal transplant experiments to examine what abiotic and biotic factors influence variation in lace bug density, and what ecological and evolutionary factors predict the resistance of the host plant between and within the native and invaded ranges...
August 3, 2017: Ecology
https://www.readbyqxmd.com/read/28770430/effect-of-selenium-application-on-arsenic-uptake-in-rice-oryza-sativa-l
#12
Sumandeep Kaur, Dhanwinder Singh, Kuldip Singh
Alluvial aquifers of the agrarian state of Punjab of southwestern arid zone used for irrigation of rice crops are rich in arsenic concentration. In the present study, rice (Oryza sativa L.) crops were raised in pots in a greenhouse with a purpose to study whether selenium (Se) application was effective in ameliorating As uptake. The rice crop was irrigated with arsenic laced water (0, 2.5, 5.0, 10.0 μM As L(-1)) throughout the growing period, without and with selenium (0.05 and 0.10 mg kg(-1)) added through mustard biomass, grown ex situ in seleniferous soil...
September 2017: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/28750417/prediction-of-potentially-avoidable-readmission-risk-in-a-division-of-general-internal-medicine
#13
Marc Uhlmann, Estelle Lécureux, Anne-Claude Griesser, Hong Dung Duong, Olivier Lamy
INTRODUCTION: The 30-day post-discharge readmission rate is a quality indicator that may reflect suboptimal care. The computerised algorithm SQLape® can retrospectively identify a potentially avoidable readmission (PARA) with high sensitivity and specificity. We retrospectively analysed the hospital stays of patients readmitted to the Department of Internal Medicine of the CHUV (Centre Hospitalier Universitaire Vaudois) in order to quantify the proportion of PARAs and derive a risk prediction model...
July 27, 2017: Swiss Medical Weekly
https://www.readbyqxmd.com/read/28747875/effects-of-high-definition-anodal-transcranial-direct-current-stimulation-applied-simultaneously-to-both-primary-motor-cortices-on-bimanual-sensorimotor-performance
#14
Nils H Pixa, Fabian Steinberg, Michael Doppelmayr
Many daily activities, such as tying one's shoe laces, opening a jar of jam or performing a free throw in basketball, require the skillful coordinated use of both hands. Even though the non-invasive method of transcranial direct current stimulation (tDCS) has been repeatedly shown to improve unimanual motor performance, little is known about its effects on bimanual motor performance. More knowledge about how tDCS may improve bimanual behavior would be relevant to motor recovery, e.g., in persons with bilateral impairment of hand function...
2017: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/28743602/menthol-disrupts-nicotine-s-psychostimulant-properties-in-an-age-and-sex-dependent-manner-in-c57bl-6j-mice
#15
Benjamin W Fait, David C Thompson, Tenna N Mose, Peter Jatlow, Sven E Jordt, Marina R Picciotto, Yann S Mineur
Menthol is a commonly used flavorant in tobacco and e-cigarettes, and could contribute to nicotine sensitivity. To understand how menthol could contribute to nicotine intake and addiction, it is important to determine whether specific mechanisms related to sex and age could underlie behavioral changes induced by menthol-laced nicotinic products. Using a validated paradigm of nicotine-dependent locomotor stimulation, adolescent and adult C57BL/6J mice of both sexes were exposed to nicotine, or nicotine laced with menthol, as their sole source of fluid, and psychostimulant effects were evaluated by recording home cage locomotor activity for ten days...
September 15, 2017: Behavioural Brain Research
https://www.readbyqxmd.com/read/28742370/a-community-grows-around-the-geysering-world-of-enceladus
#16
Carolyn C Porco
The discovery by NASA's Cassini mission at Saturn in 2005 of a large plume of material erupting from the south polar terrain of Enceladus, sourced within a subsurface ocean of salty liquid water laced with organic compounds, has brought together scientists from a diverse range of disciplines over the last decade to evaluate this small moon's potential for extraterrestrial life. The collection of papers published today in Astrobiology, as the mission draws to a close, is the outcome of our most recent meeting at UC Berkeley in June 2016...
September 2017: Astrobiology
https://www.readbyqxmd.com/read/28728975/continuous-nitrogen-removal-by-a-single-stage-reactor-packed-with-ring-laced-string-medium
#17
Masahiro Tatara, Shu Ishikawa, Yoshiyuki Ueno
The efficiency of nitrogen removal by a partial-nitritation/anammox (PNA) reaction was investigated using a packed-bed reactor in which ring-laced strings were used as the supporting medium. A stable population of PNA microorganisms was established from typical activated sludge, after less than two months of acclimation in the packed-bed reactor, by applying a high nitrogen-loading rate (NLR: 0.53 kg/m(3)/d) and short hydraulic retention time (HRT: 1.8 h). The stability of reactor performance was confirmed in industrial wastewater (IW), demonstrating a nitrogen removal efficiency (NRE) of greater than 77% during 260 days of continuous operation, between 0...
July 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28710226/evaluating-the-predictive-strength-of-the-lace-index-in-identifying-patients-at-high-risk-of-hospital-readmission-following-an-inpatient-episode-a-retrospective-cohort-study
#18
Sarah Damery, Gill Combes
OBJECTIVE: To assess how well the LACE index and its constituent elements predict 30-day hospital readmission, and to determine whether other combinations of clinical or sociodemographic variables may enhance prognostic capability. DESIGN: Retrospective cohort study with split sample design for model validation. SETTING: One large hospital Trust in the West Midlands. PARTICIPANTS: All alive-discharge adult inpatient episodes between 1 January 2013 and 31 December 2014...
July 13, 2017: BMJ Open
https://www.readbyqxmd.com/read/28709684/hairpin-structures-with-conserved-sequence-motifs-determine-the-3-ends-of-non-polyadenylated-invertebrate-iridovirus-transcripts
#19
İkbal Agah İnce, Gorben P Pijlman, Just M Vlak, Monique M van Oers
Previously, we observed that the transcripts of Invertebrate iridescent virus 6 (IIV6) are not polyadenylated, in line with the absence of canonical poly(A) motifs (AATAAA) downstream of the open reading frames (ORFs) in the genome. Here, we determined the 3' ends of the transcripts of fifty-four IIV6 virion protein genes in infected Drosophila Schneider 2 (S2) cells. By using ligation-based amplification of cDNA ends (LACE) it was shown that the IIV6 mRNAs often ended with a CAUUA motif. In silico analysis showed that the 3'-untranslated regions of IIV6 genes have the ability to form hairpin structures (22-56 nt in length) and that for about half of all IIV6 genes these 3' sequences contained complementary TAATG and CATTA motifs...
November 2017: Virology
https://www.readbyqxmd.com/read/28708848/predicting-all-cause-risk-of-30-day-hospital-readmission-using-artificial-neural-networks
#20
Mehdi Jamei, Aleksandr Nisnevich, Everett Wetchler, Sylvia Sudat, Eric Liu
Avoidable hospital readmissions not only contribute to the high costs of healthcare in the US, but also have an impact on the quality of care for patients. Large scale adoption of Electronic Health Records (EHR) has created the opportunity to proactively identify patients with high risk of hospital readmission, and apply effective interventions to mitigate that risk. To that end, in the past, numerous machine-learning models have been employed to predict the risk of 30-day hospital readmission. However, the need for an accurate and real-time predictive model, suitable for hospital setting applications still exists...
2017: PloS One
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