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crosslink cornea

Valeria Perugini, Anna L Guildford, Joana Silva-Correia, Joaquim M Oliveira, Steven T Meikle, Rui L Reis, Matteo Santin
Damage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers is limited due to their short half-life in vivo. In this work, an enhanced stability and bioavailability of a known VEGF blocker aptamer sequence (WHLPFKC) was pursued through its tethering of molecular scaffolds based on hyperbranched peptides, the poly(ɛ-lysine) dendrons, bearing three branching generations...
October 8, 2016: Journal of Tissue Engineering and Regenerative Medicine
Vipuil Kishore, Ranjani Iyer, Athela Frandsen, Thuy-Uyen Nguyen
Loss of vision due to corneal disease is a significant problem worldwide. Transplantation of donor corneas is a viable treatment option but limitations such as short supply and immune-related complications call for alternative options for the treatment of corneal disease. A tissue engineering-based approach using a collagen scaffold is a promising alternative to develop a bioengineered cornea that mimics the functionality of native cornea. In this study, an electrochemical compaction method was employed to synthesize highly dense and transparent collagen matrices...
October 6, 2016: Biomedical Materials
Patrícia A Bersanetti, Tatiane L N Bueno, Andreia de A Morandim-Giannetti, Regina F Nogueira, Jivaldo R Matos, Paulo Schor
PURPOSE: In this study, we characterized rabbit corneas subjected to corneal cross-linking (CXL) with açaí extract compared with a riboflavin photo-stimulated procedure. MATERIALS AND METHODS: The corneas of the slaughterhouse rabbits were divided into three groups: control, consisting of untreated corneal samples; riboflavin/UVA, where corneas were treated with 0.1% riboflavin photo-stimulated at 365 nm as the standard protocol; and açaí, where the samples were subjected to 4% açaí extract for 0...
September 9, 2016: Current Eye Research
Leon C Ho, Ian A Sigal, Ning-Jiun Jan, Xiaoling Yang, Yolandi van der Merwe, Yu Yu, Ying Chau, Christopher K Leung, Ian P Conner, Tao Jin, Ed X Wu, Seong-Gi Kim, Gadi Wollstein, Joel S Schuman, Kevin C Chan
The microstructural organization and composition of the corneoscleral shell (CSS) determine the biomechanical behavior of the eye, and are important in diseases such as glaucoma and myopia. However, limited techniques can assess these properties globally, non-invasively and quantitatively. In this study, we hypothesized that multi-modal magnetic resonance imaging (MRI) can reveal the effects of biomechanical or biochemical modulation on CSS. Upon intraocular pressure (IOP) elevation, CSS appeared hyperintense in both freshly prepared ovine eyes and living rat eyes using T2-weighted MRI...
2016: Scientific Reports
Manmohan Singh, Jiasong Li, Srilatha Vantipalli, Shang Wang, Zhaolong Han, Achuth Nair, Salavat R Aglyamov, Michael D Twa, Kirill V Larin
The mechanical properties of tissues can provide valuable information about tissue integrity and health and can assist in detecting and monitoring the progression of diseases such as keratoconus. Optical coherence elastography (OCE) is a rapidly emerging technique, which can assess localized mechanical contrast in tissues with micrometer spatial resolution. In this work we present a noncontact method of optical coherence elastography to evaluate the changes in the mechanical properties of the cornea after UV-induced collagen cross-linking...
May 2016: IEEE Journal of Selected Topics in Quantum Electronics
Marcel Y Avila, Mauricio Narvaez, Juan P Castañeda
PURPOSE: To evaluate the effect of genipin, a natural crosslinking agent, in rabbit eyes. SETTING: Department of Ophthalmology, Universidad Nacional de Colombia Centro de Tecnologia Oftalmica, Bogotá, Colombia. DESIGN: Experimental study. METHODS: Ex vivo rabbit eyes (16; 8 rabbits) were treated with genipin 1.00%, 0.50%, and 0.25% for 5 minutes with a vacuum device to increase corneal permeability. Penetration was evaluated using Scheimpflug pachymetry (Pentacam)...
July 2016: Journal of Cataract and Refractive Surgery
Bia Z Kim, Charlotte A Jordan, Charles N J McGhee, Dipika V Patel
PURPOSE: To analyze corneal haze after corneal collagen crosslinking (CXL) for progressive keratoconus using Scheimpflug densitometry. SETTING: Auckland District Health Board, Auckland, New Zealand. DESIGN: Prospective randomized controlled study. METHODS: Both eyes of all patients were examined preoperatively and 1, 3, 6, and 12 months postoperatively. One eye of each patient was treated with corneal CXL, with the contralateral eye serving as the control...
July 2016: Journal of Cataract and Refractive Surgery
Joyce L Covre, Priscila C Cristovam, Renata R Loureiro, Rossen M Hazarbassanov, Mauro Campos, Élcio H Sato, José Álvaro P Gomes
PURPOSE: To culture quiescent human keratocytes and evaluate the effects of ultraviolet light and riboflavin on human corneal keratocytes in vitro. METHODS: Keratocytes were obtained from remaining corneoscleral ring donor corneas previously used in corneal transplant surgeries and cultured in DMEM/F12 with 2% FBS until confluence. Characterization of cultured cells was performed by immunofluorescence analysis for anti-cytokeratin-3, anti-Thy-1, anti-α-smooth muscle actin, and anti-lumican...
May 2016: Arquivos Brasileiros de Oftalmologia
Ahmet Kırgız, Kürşat Atalay, Kübra Şerefoğlu Çabuk, Havva Kaldırım, Muhittin Taşkapılı
PURPOSE: The present study aimed to report the outcomes of patients with progressive keratoconus who were treated via accelerated crosslinking (CXL) 6 months earlier and to determine the factors that promoted improved visual acuity after treatment. METHODS: This retrospective study included 35 eyes of 34 patients with progressive keratoconus who underwent CXL. Topographical measurements were obtained preoperatively and in the first, third, and sixth months postoperatively using a rotating Scheimpflug camera...
May 2016: Arquivos Brasileiros de Oftalmologia
Hong Zhu, Clemens Alt, Robert H Webb, Samir Melki, Irene E Kochevar
PURPOSE: To evaluate crosslinking of cornea in vivo using green light activation of Rose Bengal (RGX) and assess potential damaging effects of the green light on retina and iris. METHODS: Corneas of Dutch belted rabbits were de-epithelialized, then stained with Rose Bengal and exposed to green light, or not further treated. Corneal stiffness was measured by uniaxial tensiometry. Re-epithelialization was assessed by fluorescein fluorescence. Keratocytes were counted on hematoxylin and eosin (H&E)-stained sections, and iris cell damage was assessed by lactate dehydrogenase staining...
September 2016: Cornea
S J Lang, T Reinhard
Keratoconus leads to progressive thinning and protrusion of the cornea. Young patients exhibit the highest risk for progression. Corneal crosslinking was introduced in 1998 and is intended to prevent progression of keratoconus. Only a few prospective controlled trials have been published. Six randomised controlled trials have been published. All these trials confirmed the efficacy of crosslinking through keratometric data. In two trials, progression was reported in some patients of the treatment group. This indicates that not all patients benefit from corneal crosslinking...
June 2016: Klinische Monatsblätter Für Augenheilkunde
C J Rapuano
Iatrogenic corneal ectasia is a rare but devastating complication after refractive surgery. While its incidence appears to be declining, it has not been eliminated. Its cause is probably related to a combination of an intrinsic predisposition to ectasia and an additional anatomical destabilising effect from the refractive surgery. Determining which eyes are intrinsically "weak" and exactly how much additional "weakening" an eye can withstand before developing progressive thinning and protrusion are both difficult tasks...
June 2016: Klinische Monatsblätter Für Augenheilkunde
Barac Ileana Ramona, Corbu Catalina, Merticariu Andrei, Stefan Daciana, Tataru Calin
OBJECTIVE: The evaluation of the efficiency of corneal cross linking in the management of corneal ulcers. METHOD: A prospective study that included 10 patients, 10 eyes, with chronic corneal ulcer, bacterial and/ or fungal. The patients were divided into two groups. Group A included 5 patients with unperforated corneal ulcer and group B included 5 patients with perforated corneal ulcer. These patients were treated with general and local antibiotic and antifungal drugs, but the response was poor after two weeks...
January 2016: Romanian Journal of Ophthalmology
Elan Rosenblat, Peter S Hersh
PURPOSE: To determine intraoperative changes in corneal thickness and outcomes of corneal collagen crosslinking (CXL) using 2 intraoperative regimens: riboflavin-dextran or hypotonic riboflavin. SETTING: Cornea and refractive surgery practice, Teaneck, New Jersey, USA. DESIGN: Prospective randomized case series. METHODS: Eyes with keratoconus or corneal ectasia were treated. All eyes received preloading with riboflavin 0...
April 2016: Journal of Cataract and Refractive Surgery
Nurullah Cagil, Ozge Sarac, Gamze Dereli Can, Emine Akcay, Mehmet Erol Can
The purpose of the study was to evaluate the outcomes and possible complications of CXL performed with customized epithelial debridement technique to keratoconic corneas with the thinnest pachymetry values less than 400 µm. Nineteen eyes of 19 patients were included. The uncorrected (UCVA) and best corrected visual acuity (BCVA), flattest and steepest keratometric (K) readings, central corneal thickness at the thinnest point (t-CCT), endothelial cell density (ECD) were assessed before and 12 months after CXL...
April 21, 2016: International Ophthalmology
Nada H Aldahlawi, Sally Hayes, David P S O'Brart, Alina Akhbanbetova, Stacy L Littlechild, Keith M Meek
PURPOSE: To investigate the effect of various riboflavin/ultraviolet light (UVA) crosslinking (CXL) protocols on corneal enzymatic resistance. METHODS: A total of 66 enucleated porcine eyes, with the corneal epithelium removed, were divided into 6 groups. Group 1 remained untreated. Groups 2 to 6 received riboflavin/dextran for 30 minutes. Group 3 underwent standard CXL (SCXL) with 3 mW/cm(2) UVA for 30 minutes (total energy dose 5.4 J/cm(2)). Groups 4 and 5 underwent high intensity CXL (HCXL) using 30 mW/cm(2) UVA for 3 minutes (5...
April 2016: Investigative Ophthalmology & Visual Science
George D Kymionis, Anna I Kouroupaki, Dimitrios A Liakopoulos, Ivana R Arandjelovic, Konstantinos I Tsoulnaras
PURPOSE: To describe a case of multiorganism, drug-resistant keratitis that responded well to corneal crosslinking (CXL) treatment. METHODS: A 52-year-old man presented to our institute with a 2-month ocular history of infectious keratitis in his right eye, on topical antibacterial, antifungal, and antiprotozoan treatment, and complained of increase in pain, discomfort, and visual deterioration. Slit-lamp examination revealed advanced infectious keratitis (extensive deep corneal infiltrate with perineuritis)...
January 22, 2016: European Journal of Ophthalmology
Nacim Bouheraoua, Lea Jouve, Vincent Borderie, Laurent Laroche
Keratoconus is a bilateral and progressive corneal ectasia. In order to slow down its progression, corneal collagen cross-linking (CXL) has recently been introduced as an efficient treatment option. In biological and chemical sciences, crosslinking refers to new chemical bonds formed between reactive molecules. Hence, the aim of corneal collagen CXL is to synthetically increase the formation of crosslinks between collagen fibrils in the corneal stroma. Despite the fact that the efficiency of the conventional CXL (C-CXL) protocol has already been shown in several clinical studies, it might benefit from improvements in duration of the procedure and removal of corneal epithelium...
2015: Journal of Visualized Experiments: JoVE
Shreesha Kumar Kodavoor, Nikit J Sarwate, D Ramamurhy
A deep stromal infiltrate with hypopyon appeared in central cornea of right eye of a 15-year-old boy postoperatively after 2 days, who underwent uneventful accelerated corneal collagen crosslinking (C3R) with riboflavin and ultraviolet-A (UVA) for the treatment of keratoconus. Staphylococcus aureus keratitis was confirmed by the microbiological studies, which guided intense treatment with topical and systemic antibiotics. Before C3R, the best corrected visual acuity (BCVA) in the ocular dexter was 20/30 with the refraction of - 1...
May 2015: Oman Journal of Ophthalmology
Sanja Sefic Kasumovic, Milka Mavija, Aida Kasumovic, Orhan Lepara, Belkisa Duric-Colic, Emir Cabric, Lejla Muhamedagic, Adisa Sakovic-Racic, Mirko Jankov
AIM: To determine the possible relation between intraocular pressure (IOP), central corneal thickness (CCT) and corneal resistance (CR) in kerotoconic eyes before, 3,6 and 12 months after collagen crosslinking procedure (CXL) with aim to find out does the thicker cornea means already more resistance cornea followed with higher IOP. METHODS: Thirty eyes (30 patients) with central keratoconus (KC)were evaluated in retrospective cross sectional study. The corneal biomechanical parameters were taken with Wave Light Allegro Oculyzer produced by Alcon before the CXL, 3, 6 and 12 months after the procedure...
October 2015: Medical Archives
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