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https://www.readbyqxmd.com/read/27930708/three-dimensional-construction-of-a-rabbit-anterior-corneal-replacement-for-lamellar-keratoplasty
#1
Kunpeng Pang, Liqun Du, Kai Zhang, Chenyang Dai, Chengqun Ju, Jing Zhu, Xinyi Wu
The aim of this study was to construct a rabbit anterior corneal replacement for transplantation using acellular porcine corneal matrix (APCM) and rabbit epithelial or stromal cells. APCM was prepared from fresh porcine cornea treated with 0.5% (wt./vol.) sodium dodecyl sulfate (SDS) solution. The expanded stromal cells were first injected into APCM parallel to its surface and were cultured in a shaking culture system for 7 days to obtain the stromal construct. Next, corneal epithelial cells were cultured on the stromal construct surface for another 7 days to obtain rabbit anterior corneal lamella...
2016: PloS One
https://www.readbyqxmd.com/read/27898982/evaluation-of-corneal-cross-linking-for-treatment-of-fungal-keratitis-using-confocal-laser-scanning-microscopy-on-an-ex-vivo-human-corneal-model
#2
Jawaher M Alshehri, David Caballero-Lima, M Chantal Hillarby, Susan G Shawcross, Arun Brahma, Fiona Carley, Nick D Read, Hema Radhakrishnan
Purpose: Some previous reports have established the use of photoactivated chromophore-induced corneal cross-linking (PACK-CXL) in treating fungal keratitis. The results of these case reports have often been conflicting. To systematically study the effect of PACK-CXL in the management of Fusarium keratitis, we have developed an ex vivo model of human corneal infection using eye-banked human corneas. Methods: Sixteen healthy ex vivo human corneas were divided into four study groups: (1) untreated control, (2) cross-linked, (3) infected with fungal spores, and (4) infected with fungal spores and then cross-linked...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27856177/diagnostic-criteria-for-limbal-stem-cell-deficiency-a-systematic-literature-review
#3
REVIEW
Lona Jawaheer, Deepa Anijeet, Kanna Ramaesh
PURPOSE: The diagnosis of limbal stem cell deficiency (LSCD) is often based on clinical manifestations with or without the use of tests to demonstrate the presence of goblet cells or of specific epithelial markers on the corneolimbal surface. This systematic review looks at the various diagnostic methods employed in the diagnosis of LSCD in published interventional studies. DESIGN: Systematic literature review METHODS: We did a systematic search on MEDLINE and PUBMED for articles published in English between January 1, 2003 and December 31, 2013...
November 14, 2016: Survey of Ophthalmology
https://www.readbyqxmd.com/read/27849309/activation-of-rac1-and-rhoa-preserve-corneal-endothelial-barrier-function
#4
María C Ortega, Diana Santander-García, Beatriz Marcos-Ramiro, Susana Barroso, Susan Cox, Ignacio Jiménez-Alfaro, Jaime Millán
Purpose: The corneal endothelium is responsible for the correct hydration of the corneal stroma. Corneal endothelial cells have a low proliferative capacity, so preserving their barrier function under suboptimal conditions that cause osmotic imbalance, such as those arising from corneal pathologies, age, cryopreservation, and transplantation, is essential for maintaining corneal transparency. We have investigated the signaling induced by hyperosmotic shock that reversibly disrupts corneal endothelial barriers in human endothelial cells and in murine corneas...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27843433/successful-treatment-of-lasiodiplodia-theobromae-keratitis-assessing-the-role-of-voriconazole
#5
Stephen Tak-Lun Li, Evan Po-Fat Yiu, Angela Hiu-Yan Wong, John Chun-Ting Yeung, Lester Wang-Hon Yu
PURPOSE: The aim of this paper is to report the successful management of the first case of Lasiodiplodia theobromae keratitis in Hong Kong. METHODS: We conducted a case report. RESULTS: A 43-year-old Chinese male with a history of diabetes developed left eye keratitis after a trauma during tree felling. Fungal keratitis was diagnosed using a confocal microscope on day 1, and L. theobromae was confirmed from the culture. He was given oral voriconazole, topical natamycin, and topical and intracameral amphotericin B...
September 2016: Case Reports in Ophthalmology
https://www.readbyqxmd.com/read/27820631/in-vivo-distribution-of-corneal-epithelial-dendritic-cells-in-patients-with-glaucoma
#6
Rodolfo Mastropasqua, Luca Agnifili, Vincenzo Fasanella, Andrea Lappa, Lorenza Brescia, Manuela Lanzini, Francesco Oddone, Paolo Perri, Leonardo Mastropasqua
Purpose: The purpose of this study was to evaluate dendritic cell (DC) distribution, morphology, and DC density in the entire cornea of medically controlled glaucoma patients (MCGP), using in vivo confocal microscopy (IVCM). Methods: Fifty MCGP were enrolled, 15 patients with dry eye, and 15 healthy subjects served as controls. Patients were asked to complete the Ocular Surface Disease Index (OSDI) questionnaire and then underwent tear film break-up time (BUT), corneal staining, and Schirmer test (ST) I and then IVCM...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27816571/corneal-nerve-and-epithelial-cell-alterations-in-corneal-allodynia-an-in-vivo-confocal-microscopy-case-series
#7
Pedram Hamrah, Yureeda Qazi, Bashar Shahatit, Mohammad H Dastjerdi, Deborah Pavan-Langston, Deborah S Jacobs, Perry Rosenthal
PURPOSE: To investigate the morphological changes of the corneal epithelium and sub-basal nerves in patients with corneal allodynia using in vivo confocal microscopy (IVCM). DESIGN: Case-control study of patients with corneal allodynia and healthy controls. METHODS: Ten eyes of six patients were diagnosed with corneal allodynia at a single center and compared to fifteen healthy eyes. IVCM of the central cornea was performed on all subjects and controls...
November 2, 2016: Ocular Surface
https://www.readbyqxmd.com/read/27813513/comparative-quantitative-assessment-of-the-human-corneal-sub-basal-nerve-plexus-by-in-vivo-confocal-microscopy-and-histological-staining
#8
B S Kowtharapu, K Winter, C Marfurt, S Allgeier, B Köhler, M Hovakimyan, T Stahnke, A Wree, O Stachs, R F Guthoff
PurposeThis study was designed to compare and contrast quantitative data of the human corneal sub-basal nerve plexus (SBP) evaluated by two different methods: in vivo confocal microscopy (IVCM), and immunohistochemical staining of ex vivo donor corneas.MethodsSeven parameters of the SBP in large-scale IVCM mosaicking images from healthy subjects were compared with the identical parameters in ex vivo donor corneas stained by β-III-tubulin immunohistochemistry. Corneal nerve fiber length (CNFL), corneal nerve fiber density (CNFD), corneal nerve branch density (CNBD), average weighted corneal nerve fiber tortuosity (CNFTo), corneal nerve connection points (CNCP), average corneal nerve single-fiber length (CNSFL), and average weighted corneal nerve fiber thickness (CNFTh) were calculated using a dedicated, published algorithm and compared...
November 4, 2016: Eye
https://www.readbyqxmd.com/read/27811567/patients-with-dry-eye-disease-and-low-subbasal-nerve-density-are-at-high-risk-for-accelerated-corneal-endothelial-cell-loss
#9
Ahmad Kheirkhah, Vannarut Satitpitakul, Pedram Hamrah, Reza Dana
PURPOSE: To evaluate changes in corneal endothelial cell density over time in patients with dry eye disease (DED) and to correlate endothelial cell loss with corneal subbasal nerve density. METHODS: This retrospective study included 40 eyes of 20 patients with DED. Laser in vivo confocal microscopy had been performed in the central cornea of both eyes at an initial visit and repeated after a mean follow-up of 33.2 ± 10.2 months. The densities of corneal endothelial cells and subbasal nerves were measured in both visits and compared with 13 eyes of 13 normal age-matched controls...
November 2, 2016: Cornea
https://www.readbyqxmd.com/read/27811337/patients-with-multiple-sclerosis-demonstrate-reduced-subbasal-corneal-nerve-fibre-density
#10
Janine Mikolajczak, Hanna Zimmermann, Ahmad Kheirkhah, Ella Maria Kadas, Timm Oberwahrenbrock, Rodrigo Muller, Aiai Ren, Joseph Kuchling, Holger Dietze, Harald Prüss, Friedemann Paul, Pedram Hamrah, Alexander U Brandt
BACKGROUND: Many studies in multiple sclerosis (MS) have investigated the retina. Little, however, is known about the effect of MS on the cornea, which is innervated by the trigeminal nerve. It is the site of neural-immune interaction with local dendritic cells reacting in response to environmental stimuli. OBJECTIVE: This study aims to investigate the effect of MS on corneal nerve fibres and dendritic cells in the subbasal nerve plexus using in vivo confocal microscopy (IVCM)...
November 3, 2016: Multiple Sclerosis: Clinical and Laboratory Research
https://www.readbyqxmd.com/read/27774316/effect-of-treatment-with-salsalate-menhaden-oil-combination-of-salsalate-and-menhaden-oil-or-resolvin-d1-of-c57bl-6j-type-1-diabetic-mouse-on-neuropathic-endpoints
#11
Matthew S Yorek, Lawrence J Coppey, Hanna Shevalye, Alexander Obrosov, Randy H Kardon, Mark A Yorek
Aims. In this study a streptozotocin induced type 1 diabetes mouse model was used to assess the effectiveness of salsalate, menhaden oil, the combination of salsalate and menhaden oil, or resolvin D1 on neuropathic endpoints. Materials and Methods. Changes in body weight, blood glucose, serum markers for triglycerides, free fatty acids, cholesterol, and resolvin D1, motor and sensory nerve conduction velocities and thermal sensitivity were assessed, as well as performing in vivo confocal microscopy of subepithelial corneal nerves and immunohistochemistry of nerves in the cornea and foot pad...
2016: Journal of Nutrition and Metabolism
https://www.readbyqxmd.com/read/27771327/in-vivo-confocal-microscopy-of-corneal-nerves-in-health-and-disease
#12
REVIEW
Andrea Cruzat, Yureeda Qazi, Pedram Hamrah
In vivo confocal microscopy (IVCM) is becoming an indispensable tool for studying corneal physiology and disease. Enabling the dissection of corneal architecture at a cellular level, this technique offers fast and noninvasive in vivo imaging of the cornea with images comparable to that of ex vivo histochemical techniques. Corneal nerves bear substantial relevance to clinicians and scientists alike, given their pivotal roles in regulation of corneal sensation, maintenance of epithelial integrity, and proliferation and promotion of wound healing...
October 19, 2016: Ocular Surface
https://www.readbyqxmd.com/read/27760648/-study-on-the-evaluation-of-pterygium-activity-with-in-vivo-confocal-microscopy
#13
Q F Liang, C Gao, H Liang, B Li, X H Du, Antoine Labbe
Objective: To observe the characteristics of pterygium with in vivo confocal microscopy (IVCM) and understand pterygium activity with the density of inflammatory cells, formation of new blood vessels, and the number of activated keratocytes within the stroma. Methods: A prospective case-controlled study. Thirty-six pterygia from 28 patients were analyzed in this study. A pterygium activity score was obtained by summing four scores of ocular discomfort, pterygium hyperemia, keratitis, and the presence of Fuchs patches...
October 11, 2016: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/27755188/matrix-based-regenerating-agent-for-corneal-wound-healing-after-collagen-cross-linking
#14
Marina Hovakimyan, Oliver Stachs, Olmiere Céline, Rudolf F Guthoff
PURPOSE: Corneal collagen cross-linking (CXL) is associated with massive cellular damage, the long-term clinical consequences of which have still to be elucidated. In this study, we seek to determine whether matrix-based regenerating agent (RGTA) therapy has a positive influence on stromal regeneration after CXL. METHODS: Twelve New Zealand white rabbits were randomly divided into 2 groups of 6. CXL of the right cornea was performed in both groups, followed by topical corneal application of one drop of vehicle or one drop of RGTA...
October 14, 2016: Cornea
https://www.readbyqxmd.com/read/27749450/morphology-of-the-corneal-limbus-following-standard-and-accelerated-corneal-collagen-cross-linking-9-mw-cm2-for-keratoconus
#15
Ömür Ö Uçakhan, Betül Bayraktutar
PURPOSE: To evaluate the morphological features of the corneal limbus as measured by in vivo confocal microscopy (IVCM) following standard and accelerated corneal collagen cross-linking (CXL) for keratoconus. METHODS: Patients with progressive keratoconus scheduled to undergo standard CXL (group 1; 31 patients, 3 mW/cm, 370 nm, 30 minutes), or accelerated CXL (group 2; 20 patients, 9 mW/cm, 370 nm, 10 minutes) in the worse eye were included in this prospective study...
January 2017: Cornea
https://www.readbyqxmd.com/read/27709355/rapidly-dissolving-polymeric-microneedles-for-minimally-invasive-intraocular-drug-delivery
#16
Raghu Raj Singh Thakur, Ismaiel A Tekko, Farhan Al-Shammari, Ahlam A Ali, Helen McCarthy, Ryan F Donnelly
In this study, dissolving microneedles (MNs) were used to enhance ocular drug delivery of macromolecules. MNs were fabricated using polyvinylpyrrolidone (PVP) polymer of various molecular weights (MWs) containing three model molecules of increasing MW, namely fluorescein sodium and fluorescein isothiocyanate-dextrans (with MW of 70 k and 150 k Da). Arrays (3 × 3) of PVP MNs with conical shape measuring about 800 μm in height with a 300 μm base diameter, containing the model drugs, were fabricated and characterized for their fracture forces, insertion forces (in the sclera and cornea), depth of penetration (using OCT and confocal imaging), dissolution time and in vitro permeation...
December 2016: Drug Delivery and Translational Research
https://www.readbyqxmd.com/read/27701622/regeneration-of-corneal-epithelium-with-dental-pulp-stem-cells-using-a-contact-lens-delivery-system
#17
Evgeny Kushnerev, Susan G Shawcross, Shankari Sothirachagan, Fiona Carley, Arun Brahma, Julian M Yates, M Chantal Hillarby
Purpose: The corneal epithelium is sloughed off surface of the eye by the action of blinking and is continually replaced by division and maturation of the limbal stem cells (LSCs). In the case of injury or disease, LSCs can be lost or damaged to a point at which the corneal epithelial layer is no longer maintained. leading to LSC deficiencies (LSCDs). When this occurs, the opaque conjunctiva overgrows the anterior surface of the eye, leading to vision impairment or loss. Dental pulp stem cells (DPSCs) are promising candidates as autologous LSC substitutes...
October 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27676095/design-and-synthesis-of-new-cell-penetrating-peptides-diffusion-and-distribution-inside-the-cornea
#18
Silvia Pescina, Marina Sala, Cristina Padula, Maria Carmina Scala, Antonia Spensiero, Silvana Belletti, Rita Gatti, Ettore Novellino, Pietro Campiglia, Patrizia Santi, Sara Nicoli, Carmine Ostacolo
The role of cell penetrating peptides (CPPs) has been challenged in recent years for drug delivery to ocular tissues for the targeting of both anterior and posterior segments. The enhancement of trans-corneal transport for anterior segment targeting is a very important issue possibly leading to important outcomes on efficacy and to the opportunity of topical administration of molecules with unfavorable penetration properties. The aim of the present work was the design and synthesis of new CPPs, deriving from the structure of PEP-1 peptide...
October 4, 2016: Molecular Pharmaceutics
https://www.readbyqxmd.com/read/27661858/corneal-hydration-control-in-fuchs-endothelial-corneal-dystrophy
#19
Katrin Wacker, Jay W McLaren, Katrina M Kane, Keith H Baratz, Sanjay V Patel
Purpose: To assess corneal hydration control across a range of severity of Fuchs' endothelial corneal dystrophy (FECD) by measuring the percent recovery per hour (PRPH) of central corneal thickness after swelling the cornea and to determine its association with corneal morphologic parameters. Methods: Twenty-three corneas of 23 phakic FECD patients and 8 corneas of 8 healthy control participants devoid of guttae were graded (modified Krachmer scale). Effective endothelial cell density (ECDe) was determined from the area of guttae and local cell density in confocal microscopy images...
September 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27660697/in-vivo-corneal-confocal-microscopy-in-diabetes-where-we-are-and-where-we-can-get
#20
REVIEW
Ernesto Maddaloni, Francesco Sabatino
In vivo corneal confocal microscopy (IVCCM) is a novel, reproducible, easy and noninvasive technique that allows the study of the different layers of the cornea at a cellular level. As cornea is the most innervated organ of human body, several studies investigated the use of corneal confocal microscopy to detect diabetic neuropathies, which are invalidating and deadly complications of diabetes mellitus. Corneal nerve innervation has been shown impaired in subjects with diabetes and a close association between damages of peripheral nerves due to the diabetes and alterations in corneal sub-basal nerve plexus detected by IVCCM has been widely demonstrated...
September 15, 2016: World Journal of Diabetes
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