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toxoplasmosis diagnosis

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https://www.readbyqxmd.com/read/28968510/molecular-diagnosis-of-symptomatic-toxoplasmosis-a-9-year-retrospective-and-prospective-study-in-a-referral-laboratory-in-s%C3%A3-o-paulo-brazil
#1
Lilian Muniz Camilo, Vera Lucia Pereira-Chioccola, Ricardo Gava, Cristina da Silva Meira-Strejevitch, Jose Ernesto Vidal, Cinara Cássia Brandão de Mattos, Fábio Batista Frederico, Luiz Carlos de Mattos, Lígia Cosentino Junqueira Franco Spegiorin
Symptomatic forms of toxoplasmosis are a serious public health problem and occur in around 10-20% of the infected people. Aiming to improve the molecular diagnosis of symptomatic toxoplasmosis in Brazilian patients, this study evaluated the performance of real time PCR (qPCR) testing two primer sets (B1 and REP-529) in detecting Toxoplasma gondii DNA. The methodology was assayed in 807 clinical samples with known clinical diagnosis, ELISA, and conventional PCR (cPCR) results in a 9-year period. All samples were from patients with clinical suspicion of several features of toxoplasmosis...
September 29, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28964770/advantages-of-bioconjugated-silica-coated-nanoparticles-as-an-innovative-diagnosis-for-human-toxoplasmosis
#2
Ibrahim Aly, Eman E Taher, Gehan El Nain, Hoda El Sayed, Faten A Mohammed, Rabab S Hamad, Elsayed M Bayoumy
Nanotechnology is a promising arena for generating new applications in Medicine. To successfully functionalised nanoparticles for a given biomedical application, a wide range of chemical, physical and biological factors have to be taken into account. Silica-coated nanoparticles, (SiO2NP) exhibit substantial diagnostic activity owing to their large surface to volume ratios and crystallographic surface structure. This work aimed to evaluate the advantage of bioconjugation of SiO2NP with PAb against Toxoplasma lyzate antigen (TLA) as an innovative diagnostic method for human toxoplasmosis...
September 28, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28920810/newly-described-toxoplasma-gondii-strain-causes-high-mortality-in-red-necked-wallabies-macropus-rufogriseus-in-a-zoo
#3
Amanda Guthrie, Leah Rooker, Rommel Tan, Richard Gerhold, Karen Trainor, Tiantian Jiang, Chunlei Su
This manuscript describes an outbreak of fatal toxoplasmosis in wallabies. Ten adult red necked wallabies (Macropus rufogriseus) were imported from New Zealand to the Virginia Zoo. Agglutination testing upon admission into quarantine showed all animals to be negative for antibodies to Toxoplasma gondii. Nine of these wallabies died from acute toxoplasmosis within 59-565 (average 224) days after being moved onto exhibit. Clinical signs included lethargy, diarrhea, tachypnea, and ataxia that progressed rapidly; death without premonitory signs occurred in one case...
September 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/28882612/proposed-panel-of-diagnostic-tools-for-accurate-temporal-classification-of-symptomatic-t-gondii-infection
#4
Geisa Baptista Barros, Elenice Moreira Lemos, Priscila Pinto E Silva-Dos-Santos, Reynaldo Dietze, Eliana Zandonade, José Roberto Mineo, Deise Aparecida de Oliveira Silva, Ana Cláudia Marquez Pajuaba, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Jordana Grazziela Coelho-Dos-Reis, Olindo Assis Martins-Filho, José Carlos Serufo
Serological tests available for the diagnosis of acute Toxoplasma gondii infection have limitations in establishing the temporal diagnosis of acute toxoplasmosis. The present analytical-descriptive investigation comprises of a prospective longitudinal cohort study to search for accurate biomarkers to distinguish acute, early and late convalescent T. gondii infection. Classic methods (immunofluorescence-IFA along with Enzyme-linked immunosorbent-ELISA and fluorescent-ELFA assays) for IgM, IgA, IgG and IgG avidity were employed in parallel with flow cytometry-based anti-fixed T...
September 4, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28861733/patterns-of-uveitis-in-children-at-the-apex-institute-for-eye-care-in-india-analysis-and-review-of-literature
#5
Brijesh Takkar, Pradeep Venkatesh, Nripen Gaur, Sat Pal Garg, Rajpal Vohra, Supriyo Ghose
AIM: To study patterns of uveitis in Indian children and compare with data sets published earlier in the literature. METHODS: Consecutive patients below 16 years of age presenting to the uvea clinic of a tertiary eye care center were included prospectively through the period of July 2009-August 2013. Children with retinal vasculitis, exogenous endophthalmitis and masquerade syndromes were excluded from analysis. Uveitis was classified as per the nomenclature system adopted by the International Uveitis Study Group...
August 31, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/28851620/diagnosis-of-congenital-toxoplasmosis-in-a-renal-transplant-recipient-mother
#6
M Hermann, H Yéra, I Villena, B Cimon, E Thervet, A Benachi
We report the case of a first trimester toxoplasmosis infection in a renal transplant recipient. Real-time polymerase chain reaction in amniotic fluid at 18 weeks was negative for Toxoplasma gondii but at 26 weeks major fetal hydrocephalus was discovered leading to medical termination of pregnancy. Pathological examination confirmed lesions consistent with congenital toxoplasmosis. The herein case report, as well as data from the French reference centre for congenital Toxoplamosis (1835 cases in the past eight years), suggests that the strategy of management of pregnancy's first trimester Toxoplasmosis infection in patients treated by immunosuppressive therapy needs to be reconsidered...
October 2017: Journal of gynecology obstetrics and human reproduction
https://www.readbyqxmd.com/read/28835429/a-rare-case-of-pulmonary-toxoplasmosis-in-a-patient-with-undifferentiated-inflammatory-arthritis-on-chronic-methotrexate-and-corticosteroid-therapy
#7
Abdullateef Abdulkareem, Ryan Steven D'Souza, Nitin Patel, Anthony A Donato
Pulmonary toxoplasmosis is a serious pulmonary condition caused by the protozoan Toxoplasma gondii It typically affects immunocompromised patients presenting acutely with cough, fever, myalgias, arthralgias and lymphadenopathy, and chronically with persistent cough and dyspnoea. Because of its protean features, it can mimic many more common lung conditions in the immunocompromised patient, including atypical pneumonia, Pneumocystis pneumonia and interstitial lung disease. In this article, we present the case of a 55-year-old woman who presented to our hospital with persistent dyspnoea and cough, initially suspected to have an arthritis-related interstitial lung disease...
August 23, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28827190/establishing-tools-for-early-diagnosis-of-congenital-toxoplasmosis-flow-cytometric-igg-avidity-assay-as-a-confirmatory-test-for-neonatal-screening
#8
Aline de Castro Zacche-Tonini, Giuliana Schmidt França Fonseca, Laura Néspoli Nassar Pansini de Jesus, Geisa Baptista Barros, Jordana Grazziela Alves Coelho-Dos-Reis, Samantha Ribeiro Béla, Anderson Silva Machado, Ana Carolina Aguiar Vasconcelos Carneiro, Gláucia Manzan Queiroz Andrade, Daniel Vitor Vasconcelos-Santos, José Nélio Januário, Andréa Teixeira-Carvalho, Ricardo Wagner Almeida Vitor, Eloísa Amália Vieira Ferro, José Roberto Mineo, Olindo Assis Martins-Filho, Elenice Moreira Lemos
The aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™) and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO=88) and age-matching non-infected controls (NI=15).The results demonstrated that all samples tested positive/indeterminate for anti-T. gondii IgM screening at birth using air-dried whole blood samples. Serum samples collected at 30-45days after birth tested positive for ELFAVIDAS™ IgG in both groups...
August 18, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28819470/causes-of-death-and-factors-associated-with-early-mortality-of-hiv-infected-adults-admitted-to-korle-bu-teaching-hospital
#9
Adriana Saavedra, Nia Campinha-Bacote, Maurice Hajjar, Ernest Kenu, Fizza Syeda Gillani, Adjoa Obo-Akwa, Margaret Lartey, Awewura Kwara
INTRODUCTION: This study sought to identify common causes of death as well as the factors associated with the high inpatient mortality rate of HIV-infected patients at the Korle-Bu Teaching Hospital (KBTH). METHODS: The retrospective study reviewed the medical records of 547 HIV-infected adults aged 18 years or older admitted to the KBTH between the months of January 2012 and October 2013. Using standardized abstraction forms, clinical and demographic data of eligible patients was collected...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28818085/a-rare-case-of-acute-toxoplasmosis-in-a-stray-dog-due-to-infection-of-t-gondii-clonal-type-i-public-health-concern-in-urban-settings-with-stray-animals
#10
Sergio Migliore, Salvatore La Marca, Cristian Stabile, Vincenzo Di Marco Lo Presti, Maria Vitale
BACKGROUND: Typing of Toxoplasma gondii strains is important in epidemiological surveys, to understand the distribution and virulence of different clones of the parasite among human and animal populations. Stray dogs can be consider sentinel animals for contaminated environments playing an important but probably under- evaluated role in the epidemiology of T. gondii. We reported a rare case of acute toxoplasmosis in a stray dog due to clonal type I infection. The clonal type I, sporadic in Europe, is frequently associated with severe toxoplasmosis in humans and the control of its circulation is particularly relevant for public health...
August 17, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28803790/utility-of-the-cytochrome-c-oxidase-subunit-i-gene-for-the-diagnosis-of-toxoplasmosis-using-pcr
#11
Xue Feng, Kazumi Norose, Kexin Li, Kenji Hikosaka
Toxoplasmosis is caused by the protozoan parasite Toxoplasma gondii, which belongs to the phylum Apicomplexa. Since this parasite causes severe clinical symptoms in immunocompromised patients, early diagnosis of toxoplasmosis is essential. PCR is currently used for early diagnosis, but there is no consensus regarding the most effective method for amplifying Toxoplasma DNA. In this study, we considered the utility of the cytochrome c subunit I (cox1) gene, which is encoded in the mitochondrial DNA of this parasite, as a novel target of PCR for the diagnosis of toxoplasmosis...
August 10, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28793826/toxoplasma-appendicitis-a-once-in-a-lifetime-diagnosis
#12
Anisha M Fernandes, Maheshkumar M Lakhe, Sanjay A Pai
Toxoplasmosis is generally asymptomatic in immunocompetent individuals, but it can be life-threatening in immunocompromised patients. We present a case of a 62-year-old man with clinical features of acute appendicitis. Histology showed a transmural infiltrate of eosinophils. In addition, there were reactive lymphoid follicles with histiocytes in the submucosa and tachyzoites in the muscularis propria. Immunohistochemistry confirmed the diagnosis of toxoplasma appendicitis. Serological evaluation yielded negative results...
August 1, 2017: International Journal of Surgical Pathology
https://www.readbyqxmd.com/read/28765144/comparing-optical-coherence-tomography-findings-in-different-aetiologies-of-infectious-necrotising-retinitis
#13
Alessandro Invernizzi, Aniruddha Kishandutt Agarwal, Vittoria Ravera, Chiara Mapelli, Agostino Riva, Giovanni Staurenghi, Peter J McCluskey, Francesco Viola
AIMS: To compare optical coherence tomography (OCT) features of active necrotising infectious retinitis (NIR) due to toxoplasmosis or herpesviruses and to determine distinctive OCT signs for these two causes of infectious retinitis. METHODS: OCT scans from eyes with active NIR due to varicella zoster virus (VZV), herpes simplex virus (HSV), cytomegalovirus (CMV), and toxoplasmosis (TOXO) were reviewed. All images were evaluated for the presence of previously described OCT findings in TOXO-NIR and compared with the viral group...
August 1, 2017: British Journal of Ophthalmology
https://www.readbyqxmd.com/read/28761461/pcr-based-diagnosis-of-toxoplasma-parasite-in-ocular-infections-having-clinical-indications-of-toxoplasmosis
#14
Atieh Farhadi, Ali Haniloo, Asghar Fazaeli, Siamak Moradian, Mehdi Farhadi
BACKGROUND: The diagnosis of ocular toxoplasmosis is mainly based on clinical features. However, ocular fluid testing by PCR may be very helpful for approval or rejection of this etiology. In this study, we utilized a nested-PCR technique, targeting the B1 partial sequence to analyze the aqueous and vitreous samples for evaluating the presence of the Toxoplasma DNA. METHODS: Fifty aqueous or vitreous humor samples were obtained from patients with clinical features of ocular toxoplasmosis admitted to ophthalmology hospitals and clinics in Iran, within 2014...
January 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28728858/-dress-complicated-by-hemophagocytic-lymphohistiocytosis-in-an-infant-treated-for-congenital-toxoplasmosis
#15
S Rioualen, J Dufau, C Flatres, P Lavenant, L Misery, J-M Roué
BACKGROUND: Drug reaction with eosinophilia and systemic symptoms (DRESS) is a severe disease that may be complicated by hemophagocytic lymphohistiocytosis but this is rarely described in children. PATIENTS AND METHODS: We report the case of a 5-week old infant hospitalized in a pediatric intensive care unit for hemophagocytic lymphohistiocytosis with prolonged fever, splenomegaly, cytopenia, fibrinogen≤1.5g/L, ferritin≥500μg/L, and soluble IL-2 receptor≥2400U/mL...
July 17, 2017: Annales de Dermatologie et de Vénéréologie
https://www.readbyqxmd.com/read/28719949/seroprevalence-of-toxoplasmosis-with-elisa-and-rapid-diagnostic-test-among-residents-in-gyodong-do-inchon-city-korea-a-four-year-follow-up
#16
Yeong Hoon Kim, Ji Hoo Lee, Seong Kyu Ahn, Tong-Soo Kim, Sung-Jong Hong, Chom-Kyu Chong, Hye-Jin Ahn, Ho-Woo Nam
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera...
June 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28713605/diarrhea-as-a-presenting-symptom-of-disseminated-toxoplasmosis
#17
Matthew Glover, Zhouwen Tang, Robert Sealock, Shilpa Jain
Disseminated toxoplasmosis is uncommon in both immunocompetent and immunocompromised hosts with gastrointestinal involvement being rarely described. We report a case of disseminated gastrointestinal toxoplasmosis in an immunocompromised man who presented with one month of diarrhea and abdominal pain. Imaging showed thickening of the ascending colon and cecum. Esophagogastroduodenoscopy and colonoscopy biopsies revealed Toxoplasma gondii, confirmed by immunostain. Symptoms completely resolved following treatment with pyrimethamine, sulfadiazine, and leucovorin...
2017: Case Reports in Gastrointestinal Medicine
https://www.readbyqxmd.com/read/28700250/false-negative-toxoplasma-serology-in-an-immunocompromised-patient-with-pcr-positive-ocular-toxoplasmosis
#18
Rehan Rajput, Alastair K Denniston, Philip I Murray
PURPOSE: The authors report a case of a 60-year-old Caucasian male with a background of treated Chronic Lymphocytic Leukaemia (CLL) with secondary hypogammaglobulinaemia present with toxoplasma chorioretinitis and negative serum toxoplasma serology on presentation and on subsequent reactivation. METHODS: Retrospective case notes review with fundal photographs. RESULTS: In this case, on initial presentation and on recurrence, the patient's serum anti-Toxoplasma IgG remained negative...
July 12, 2017: Ocular Immunology and Inflammation
https://www.readbyqxmd.com/read/28697120/association-of-parasite-load-levels-in-amniotic-fluid-with-clinical-outcome-in-congenital-toxoplasmosis
#19
Lidia Yamamoto, Lília S Targa, Laura M Sumita, Paulo T Shimokawa, Jonatas C Rodrigues, Kelly A Kanunfre, Thelma S Okay
OBJECTIVE: To correlate neonatal and infant clinical outcome with parasite load in amniotic fluid (AF). METHODS: We conducted a retrospective cohort study of 122 children whose mothers had toxoplasmosis during pregnancy. The children were monitored from birth to 12 months old. Stored AF samples were obtained at maternal diagnosis and tested by quantitative polymerase chain reaction. Gestational age at maternal infection, quantitative polymerase chain reaction results, neonatal anti-Toxoplasma gondii immunoglobulin (Ig) M, and clinical outcome at 12 months were correlated...
August 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28695827/experimental-toxoplasmosis-in-japanese-quails-coturnix-coturnix-japonica
#20
Ayhan Atasever, Cahit Babür, Abdullah İnci, Görkem Ekebaş, Anıl İça
OBJECTIVE: In this study, forming of experimental toxoplasmosis in quails; clinical, pathological, and serological determination of tissue lesions and bioassay techniques, which were aimed to compare them and determine pathogenesis. METHODS: A total of 120 one-year-old female quails (Coturnix coturnix japonica) were divided into oral infection, parenteral infection, and control groups. The oral group was infected with 0.5 ml inoculum suspension containing 106 tachyzoites of Toxoplasma gondii, whereas the control group was administered 0...
June 2017: Türkiye Parazitolojii Dergisi
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