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toxoplasmosis diagnosis

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https://www.readbyqxmd.com/read/28610938/toxoplasmosis-seroprevalence-in-pregnant-women-and-serological-and-molecular-screening-in-neonatal-umbilical-cord-blood
#1
Mahshad Shieh, Mojtaba Didehdar, Reza Hajihossein, Farzam Ahmadi, Zahra Eslamirad
Toxoplasmosis is a common zoonotic disease that can also be transmitted from the mother to the embryo, with the risk of congenital infection varying around the world. The aim of this study was to screen pregnant women and their neonates for toxoplasmosis by serologic and molecular methods and assess the impact of risk factors associated with toxoplasmosis on the rate of congenital infection. This study was conducted at a regional maternity hospital in Arak, the capital of the Markazi Province in Iran, during a period of six months...
June 10, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28591403/preliminary-multiplex-microarray-igg-immunoassay-for-the-diagnosis-of-toxoplasmosis-and-rubella
#2
Priscila T Baschirotto, Marco A Krieger, Leonardo Foti
BACKGROUND: During pregnancy, toxoplasmosis and rubella can cause serious damage to the mother and the foetus through vertical transmission. Early diagnosis enables implementation of health measures aimed at preventing vertical transmission and minimising damage caused by these diseases. OBJECTIVE: Here, we report the development of a multiplex assay for simultaneous detection of IgG antibodies produced during toxoplasmosis and rubella infection. METHODS: This assay is based on xMap technology...
June 2017: Memórias do Instituto Oswaldo Cruz
https://www.readbyqxmd.com/read/28591288/ocular-sarcoidosis-masked-by-positive-igm-for-toxoplasmosis
#3
Murilo Bertazzo Peres, Jacqueline Martins de Sousa, Heloisa Nascimento
We report a case of ocular sarcoidosis with positive immunoglobulin (Ig) M and IgG serology for toxoplasmosis. The patient was a young female with red painful eyes, bilateral eyelid edema, and panuveitis with periphlebitis. In laboratory testing, she was IgM and IgG positive for toxoplasmosis and anergic in the tuberculin test. Topical treatment for anterior uveitis and oral antibiotics for toxoplasmosis were started, without improvement. Orbit tomography showed increased lacrimal glands bilaterally, and chest X-ray radiographic findings were consistent with pulmonary sarcoidosis, which supported the presumed ocular sarcoidosis diagnosis...
March 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/28579301/incidence-presentation-and-outcome-of%C3%A2-toxoplasmosis-in-hiv-infected-in-the-combination-antiretroviral-therapy-era
#4
Raquel Martin-Iguacel, Magnus Glindvad Ahlström, Madeleine Touma, Frederik Neess Engsig, Nina Breinholt Stærke, Mette Stærkind, Niels Obel, Line D Rasmussen
BACKGROUND: HIV-associated incidence and prognosis of cerebral toxoplasmosis (CTX) is not well established during later years. METHODS: From the Danish HIV Cohort Study, we identified 6325 HIV-infected individuals. We assessed incidence, mortality, predictive and prognostic factors of CTX during the pre-combination antiretroviral therapy (pre-cART; 1995-1996) and cART-era (1997-2014). Adjusted incidence rate ratios (aIRR), mortality rate ratios (aMRR) and 95% confidence intervals (CI) were assessed using Poisson regression analysis...
June 1, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28575919/zika-virus-infection-in-pregnant-women-and-microcephaly
#5
Geraldo Duarte, Antonio Fernandes Moron, Artur Timerman, César Eduardo Fernandes, Corintio Mariani Neto, Gutemberg Leão de Almeida Filho, Heron Werner Junior, Hilka Flavia Barra do Espírito Santo, João Alfredo Piffero Steibel, João Bortoletti Filho, Juvenal Barreto Borriello de Andrade, Marcelo Burlá, Marcos Felipe Silva de Sá, Newton Eduardo Busso, Paulo César Giraldo, Renato Augusto Moreira de Sá, Renato Passini Junior, Rosiane Mattar, Rossana Pulcineli Vieira Francisco
From the discovery of the Zika virus (ZIKV) in 1947 in Uganda (Africa), until its arrival in South America, it was not known that it would affect human reproductive life so severely. Today, damage to the central nervous system is known to be multiple, and microcephaly is considered the tip of the iceberg. Microcephaly actually represents the epilogue of this infection's devastating process on the central nervous system of embryos and fetuses. As a result of central nervous system aggression by the ZIKV, this infection brings the possibility of arthrogryposis, dysphagia, deafness and visual impairment...
May 2017: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/28569215/diagnosis-of-swine-toxoplasmosis-by-pcr-and-genotyping-of-toxoplasma-gondii-from-pigs-in-henan-central-china
#6
Haiyan Wang, Longxian Zhang, Qinge Ren, Fuchang Yu, Yurong Yang
BACKGROUND: Toxoplasma gondii, a widely prevalent protozoan parasite, causes serious toxoplasmosis infections in humans and other animals. Among livestock, pigs are susceptible to T. gondii infection. Despite Henan being one of the biggest pig-raising provinces in China, little information exists on the epidemiology of toxoplasmosis in this location. Therefore, we molecularly characterized DNA samples from pigs in Henan. A total of 1647 samples, including 952 from dead piglets, 478 from seriously sick fattening pigs and 217 from abortion sows, were collected from different animal hospitals or pig farms from 10 different cities in Henan (2006-2008)...
May 31, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28561960/acute-respiratory-distress-syndrome-and-septic-shock-in-a-cat-with-disseminated-toxoplasmosis
#7
Natashia A Evans, Julie M Walker, Alison C Manchester, Jonathan F Bach
OBJECTIVE: To describe acute respiratory distress syndrome (ARDS) and septic shock in a cat with disseminated toxoplasmosis. CASE SUMMARY: A 2-year-old neutered male domestic shorthair cat was presented for acute respiratory distress. At the time of presentation it had been receiving cyclosporine for treatment of eosinophilic dermatitis. Thoracic radiographs revealed severe mixed nodular interstitial and alveolar patterns. An endotracheal wash was performed, which confirmed a diagnosis of pulmonary toxoplasmosis...
May 31, 2017: Journal of Veterinary Emergency and Critical Care
https://www.readbyqxmd.com/read/28545476/prenatal-diagnosis-and-prevention-of-toxoplasmosis-in-pregnant-women-in-northern-vietnam-study-protocol
#8
G Suzanne A Smit, Thi Lam Binh Vu, Trung Dung Do, Niko Speybroeck, Brecht Devleesschauwer, Elizaveta Padalko, Ellen Roets, Pierre Dorny
BACKGROUND: In Vietnam, no systematic prenatal toxoplasmosis screening is in place, and only few studies have assessed the prevalence and importance of this zoonotic parasite infection. In addition, no studies have been conducted to assess the risk factors associated with toxoplasmosis. This study protocol was developed to determine the seroprevalence of toxoplasmosis in pregnant women in Hanoi and Thai Binh, Northern Vietnam, and to evaluate the association with risk factors and congenital toxoplasmosis...
May 25, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28533649/cerebral-toxoplasmosis-masquerading-cns-lymphoma-on-fdg-pet-ct-in-post-renal-transplant-patient
#9
Anirban Mukherjee, Chandrasekhar Bal, Madhavi Tripathi, Chandan Jyoti Das, Shamim Ahmed Shamim
20 year old post renal transplant patient developed recurrent episodes of seizure. MRI revealed focal lesion in right parieto-occipital lobe with perilesional edema. FDG PET-CT revealed multiple hypermetabolic lesions in bilateral cerebral hemisphere. Subsequent biopsy from the lesion demonstrated bradyzoites of Toxoplasma gondii with inflammatory cells and thereby, a confirmatory diagnosis of cerebral toxoplasmosis was made. This case demonstrates the fact that increased FDG uptake in cerebral lesions in post transplant patient should be interpreted with caution and confirmed with histopathological correlation...
April 2017: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
https://www.readbyqxmd.com/read/28527270/-effect-of-antenatal-spiramycin-treatment-on-the-frequency-of-retinochoroiditis-due-to-congenital-toxoplasmosis-in-a-colombian-cohort
#10
Liliana María Zuluaga, John Camilo Hernández, Carlos Felipe Castaño, Jorge Hernando Donado
INTRODUCTION: Gestational toxoplasmosis is frequent and severe. There is still debate about the benefits of treatment against ocular manifestations in the newborn. Spiramycin treatment is used for this purpose, unfortunately prenatal diagnosis is sometimes delayed and pregnant women are not treated. OBJECTIVE: To describe the relationship between treatment with spiramycin during pregnancy in mothers with gestational toxoplasmosis and development of ocular toxoplasmosis in newborns...
April 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28491442/respiratory-distress-associated-with-lungworm-infection-in-a-kitten
#11
Melissa M Hawley, Lynelle R Johnson, Donato Traversa, Dan Bucy, Karen M Vernau, William Vernau
CASE SUMMARY: A 5-month-old feral kitten developed worsening respiratory signs, including tachypnea, coughing and wheezing after standard anthelmintic treatment with fenbendazole at a local shelter. The kitten was referred to the University of California, Davis, William R Pritchard Veterinary Medicine Teaching Hospital for further evaluation. Thoracic radiographs revealed a severe diffuse bronchointerstitial pattern with bronchial cuffing, ill-defined nodules and lymphadenomegaly. Differentials included infectious etiologies such as toxoplasmosis, feline infectious peritonitis and cryptococcosis...
July 2016: JFMS open reports
https://www.readbyqxmd.com/read/28481155/a-unique-case-series-of-autosomal-recessive-bestrophinopathy-exhibiting-multigenerational-inheritance
#12
Joshua S Hardin, G Bradley Schaefer, Ahmed B Sallam, M Kathryn Williams, Sami Uwaydat
INTRODUCTION: Autosomal recessive bestrophinopathy (ARB) is a retinal disease caused by biallelic mutations of the BEST1 gene. It has a variable phenotype with white flecks in the retina, multifocal yellow subretinal deposits, macular edema, choroidal neovascularization, hyperopia, and electrophysiological abnormalities. We describe a family with ARB and multigenerational inheritance. METHODS: Three generations of a Middle Eastern family (a woman, one son, and two grandchildren) were evaluated by our ocular genetics team...
May 8, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/28477099/nanomedicine-advances-in-toxoplasmosis-diagnostic-treatment-and-vaccine-applications
#13
REVIEW
João Paulo Assolini, Virginia Márcia Concato, Manoela Daiele Gonçalves, Amanda Cristina Machado Carloto, Ivete Conchon-Costa, Wander Rogério Pavanelli, Francine Nesello Melanda, Idessania Nazareth Costa
Toxoplasmosis is an infectious disease caused by the intracellular parasite Toxoplasma gondii that affects about one third of the world's population. The diagnosis of this disease is carried out by parasite isolation and host antibodies detection. However, the diagnosis presents problems in regard to test sensitivity and specificity. Currently, the most effective T. gondii treatment is a combination of pyrimethamine and sulfadiazine, although both drugs are toxic to the host. In addition to the problems that compromise the effective diagnosis and treatment of toxoplasmosis, there are no reports or indications of any vaccine capable of fully protecting against this infection...
May 5, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28433574/serodiagnosis-of-toxoplasmosis-the-effect-of-measurement-of-igg-avidity-in-pregnant-women-in-rabat-in-morocco
#14
Majda Laboudi, Abderrahim Sadak
BACKGROUND: The diagnosis of Toxoplasmosis in pregnant women during the early first trimester of pregnancy is very important for preventing congenital infection of the fetus; it will not only prevent the risk of transmitting the infection to the fetus but it will also enable to give these women a preventive treatment. In this study, the avidity test was performed on pregnant women during their first prenatal visit at the National Institute of Hygiene in Rabat, Morocco. FINDINGS: One hundred and twenty-eight sera samples were collected from 128 pregnant women between August 2015 and June 2016; these women were chosen retrospectively and were in their first four months of pregnancy...
April 20, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28410693/clinical-and-radiological-spectrum-of-intracranial-tuberculosis-a-hospital-based-study-in-northeast-india
#15
Baiakmenlang Synmon, Marami Das, Ashok K Kayal, Munindra Goswami, Jogesh Sarma, Lakshya Basumatary, Suvorit Bhowmick
Central nervous system tuberculosis (TB) is the most severe extra pulmonary TB having a high mortality and morbidity. OBJECTIVE: To study the various clinical, biochemical, and radiological spectrum of intracranial TB. MATERIALS AND METHOD: Ninety-three patients were enrolled in this prospective study after ethical clearance and consent from August 2013 to May 2015. The entire clinical course with complications and predictors of mortality were assessed. RESULTS: 36 females (38...
April 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28404997/novel-fitc-labeled-igy-antibody-fluorescence-imaging-toxoplasma-gondii-in-vitro
#16
Mehtap Sert, Rabia Cakir Koc, Yasemin Budama Kilinc
Toxoplasmosis is caused by T. gondii and can create serious health problems in humans and also worldwide economic harm. Because of the clinical and veterinary importance of toxoplasmosis, its timely and accurate diagnosis has a major impact on disease-fighting strategies. T. gondii surface antigen 1 (SAG1), an immunodominant-specific antigen, is often used as a diagnostic tool. Therefore, the aim of this study was the optimization of novel fluorescein isothiocyanate (FITC) labeling of the SAG1-specific IgY antibody to show the potential for immunofluorescence imaging of T...
April 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28394985/-chagas-disease-affecting-the-central-nervous-system-in-a-patient-with-aids-demonstrated-by-quantitative-molecular-methods
#17
Alberto Fica, Marcia Salinas, María Isabel Jercic, Jeannette Dabanch, Andrés Soto, Sergio Quintanilla, Carlos Flores
Although infrequent, Trypanosoma cruzi reactivation is possible among patients with HIV/AIDS infection that develop a tumor-like or granulomatous lesion in the CNS. We report the case of a 60 years old male patient with HIV/AIDS and low CD4 lymphocytes count with cerebellar symptoms and mild paresis, associated to supra and infratentorial hypodense lesions and positive serology tests both to T. gondii and Trypanosoma cruzi. Empirical therapy against toxoplasmosis was prescribed together with antiretroviral therapy but without a favorable response...
February 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28368078/genotyping-of-toxoplasma-gondii-strain-directly-from-human-csf-samples-of-congenital-toxoplasmosis-clinical-case
#18
Chiara Pagliuca, Gabiria Pastore, Elena Scaglione, Annalisa Migliucci, Giuseppe Maria Maruotti, Annunziata Gaetana Cicatiello, Elena Salvatore, Marco Picardi, Josè Camilla Sammartino, Maria Consiglio Buonocore, Pasquale Martinelli, Emilia Iaccarino, Roberta Colicchio, Paola Salvatore
This report describes a case of congenital toxoplasmosis in a newborn in Southern Italy. A pregnant mother had been admitted at the 20th week of her pregnancy on account of pharyngodynia and laterocervical lymphadenopathy. Although serological testing of the mother's serum documented a seroconversion with positive IgG and IgM anti-Toxoplasma antibodies during II trimester, the woman refused to perform prenatal diagnosis for congenital toxoplasmosis. Fetal ultrasound scan already showed mild asymmetrical triventricular hydrocephaly and cerebral calcifications...
April 3, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28353456/comparison-of-iif-elisa-and-igg-avidity-tests-for-the-detection-of-anti-toxoplasma-antibodies-in-single-serum-sample-from-pregnant-women
#19
Behrouz Naghili, Shamsi Abbasalizadeh, Shahin Tabrizi, Mehranghiz Rajaii, Masoumeh Akramiyan, Hossein Alikhah, Mohammad Naghavi-Behzad, Reza Piri, Farid Karkon-Shayan, Sepideh Tehrani-Ghadim
A valid estimate of test efficiency is needed to choose adequate screening and detecting strategies in diagnosis of acute toxoplasmosis. Therefore, in the present study we evaluated the efficiency of diagnostic laboratory methods to detect anti-toxoplasma antibodies in single serum samples of pregnant women by indirect immunofluorescence test (IIF), enzyme-linked immunosorbent assay (ELISA) and IgG avidity tests in north-western Iran. In an analytical-descriptive study, during March 2010 to April 2013, 391 pregnant women aged 21 to 35 years who were referred by gynaecologists or infectious disease specialists for anti-toxoplasma antibody evaluation were studied...
March 1, 2017: Le Infezioni in Medicina
https://www.readbyqxmd.com/read/28347510/-good-s-syndrome-and-congenital-toxoplasmosis-due-to-maternal-reactivation-during-pregnancy
#20
J Tahiri, F Fouyssac, O Morel, A Maatouk
INTRODUCTION: Good syndrome is a rare condition in which thymoma is associated with hypogammaglobulinemia. It is characterized by an increased susceptibility to infections. CASE REPORT: We report a woman with Good's syndrome diagnosed after severe congenital toxoplasmosis in her daughter, even though she was immunized against this infection during pregnancy. CONCLUSION: This presentation is very unusual by its early diagnosis and to our knowledge is the first report of parasitic infection in this syndrome...
May 2017: La Revue de Médecine Interne
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