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https://www.readbyqxmd.com/read/28316418/seroepidemiology-of-toxoplasma-gondii-infection-in-pregnant-women-in-west-iran-determined-by-elisa-and-pcr-analysis
#1
Gholam Reza Ahmadpour, Behrouz Ezatpour, Ramtin Hadighi, Hormoz Oormazdi, Lame Akhlaghi, Fatemeh Tabatabaei, Mehdi Azami, Mozafar Mohammadi Nejad, Hossein Mahmoudvand
Congenital toxoplasmosis can lead to severe damage for the fetus and newborn. Considering that the seroepidemiology of Toxoplasma infection in the pregnant women is poorly studied in west of Iran, the main objective of this study was to estimate the seroprevalence and potential risk factors for congenital toxoplasmosis in Delfan, Iran. In this cross-sectional study, the serum samples obtained from pregnant women who were referred to health centers for routine monitoring of the pregnancy. Totally, 264 sera were screened for IgG and IgM anti-T...
March 2017: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/28290263/diagnosis-of-toxoplasmosis-in-pregnancy-evaluation-of-latex-protein-complexes-by-immnunoagglutination
#2
Leandro E Peretti, Verónica D G Gonzalez, Iván S Marcipar, Luis M Gugliotta
The aim of this work was to obtain a reagent based on latex particles for ruling out acute toxoplasmosis in pregnant women by immunoagglutination (IA). Latex-protein complexes (LPC) were previously synthesized coupling the recombinant protein of Toxoplasma gondii P22Ag and the homogenate of the parasite to latex particles with different size, chemical functionality and charge density. LPC were tested in IA assays against a panel of 72 pregnant women serum samples. Results were analysed through receiver operating characteristic curves, determining area under the curve (AUC), sensitivity, specificity positive and negative predictive values (PPV and NPV, respectively)...
March 14, 2017: Parasitology
https://www.readbyqxmd.com/read/28288770/experimental-toxoplasma-gondii-infections-in-pigs-humoral-immune-response-estimation-of-specific-igg-avidity-and-the-challenges-of-reproducing-vertical-transmission-in-sows
#3
Walter Basso, Felix Grimm, Maja Ruetten, Vitomir Djokic, Radu Blaga, Xaver Sidler, Peter Deplazes
Ten pregnant sows were experimentally inoculated per os with T. gondii in order to investigate vertical and galactogenic transmission of the parasite and the evolution and maturation of the specific IgG humoral response in the sows and piglets. Five seronegative sows received 10(4)T. gondii (CZ isolate clone H3) sporulated oocysts during late-pregnancy (Exp. 1), three sows received 10(4) oocysts during mid-pregnancy (Exp. 2) and three sows from Exp. 1 (and two seronegative sows) were re-inoculated with 10(5) oocysts during a further pregnancy (late-pregnancy) (Exp...
March 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28223455/acute-fetal-demise-with-first-trimester-maternal-infection-resulting-from-listeria-monocytogenes-in-a-nonhuman-primate-model
#4
Bryce Wolfe, Gregory J Wiepz, Michele Schotzko, Gennadiy I Bondarenko, Maureen Durning, Heather A Simmons, Andres Mejia, Nancy G Faith, Emmanuel Sampene, Marulasiddappa Suresh, Sophia Kathariou, Charles J Czuprynski, Thaddeus G Golos
Infection with Listeria monocytogenes during pregnancy is associated with miscarriage, preterm birth, and neonatal complications, including sepsis and meningitis. While the risk of these conditions is thought to be greatest during the third trimester of pregnancy, the determinants of fetoplacental susceptibility to infection, the contribution of gestational age, and the in vivo progression of disease at the maternal-fetal interface are poorly understood. We developed a nonhuman primate model of listeriosis to better understand antecedents of adverse pregnancy outcomes in early pregnancy...
February 21, 2017: MBio
https://www.readbyqxmd.com/read/28208940/neurological-disorders-complicating-pregnancy-focus-on-obstetric-outcome
#5
Sandya Renukesh, Lavanya Rai
INTRODUCTION: Neurological disorders in pregnancy can be pregnancy related or can be caused by exacerbation of a pre-existing neurological condition or sometimes may even be detected for the first time during pregnancy in which it might be an incidental finding. The diagnosis and management of the neurological disorders in pregnancy is always a challenging task due to varied symptomatology and risks to the fetus. The evaluation and management should be performed in a stepwise fashion and requires multidisciplinary approach...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28127336/genotyping-of-toxoplasma-gondii-strains-isolated-from-patients-with-ocular-toxoplasmosis-in-iran
#6
Maryam Norouzi, Seyyed Javad Seyyed Tabaei, Maryam Niyyati, Vafa Saber, Hamed Behniafar
BACKGROUND: Toxoplasma gondii is an obligate, intracellular protozoon that develops its sexual stage in cat's intestinal epithelial cells as definitive host and develops its asexual stage in different tissues of a wide range hosts called intermediate host. The protozoon is a food-borne and worldwide parasite that can cause serious complications such as abortion in pregnant women, encephalitis, and ocular toxoplasmosis. The present study aimed to genotype T. gondii strains isolated from patients with toxoplasmic retinochoroiditis...
July 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28056829/seroprevalence-and-determinants-of-toxoplasmosis-in-pregnant-women-attending-antenatal-clinic-at-the-university-teaching-hospital-lusaka-zambia
#7
Christiana Frimpong, Mpundu Makasa, Lungowe Sitali, Charles Michelo
BACKGROUND: Toxoplasmosis is a neglected zoonotic disease which is prevalent among pregnant women especially in Africa. This study aimed to determine the seroprevalence and determinants of the disease among pregnant women attending antenatal clinic at the University Teaching Hospital (UTH). METHOD: A cross-sectional study was employed where 411 pregnant women attending antenatal clinic at UTH were interviewed using closed ended questionnaires. Their blood was also tested for Toxoplasma gondii IgG and IgM antibodies using the OnSite Toxo IgG/IgM Combo Rapid test cassettes by CTK Biotech, Inc, USA...
January 5, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28030925/comparison-of-the-efficiency-of-two-commercial-kits-elfa-and-western-blot-in-estimating-the-phase-of-toxoplasma-gondii-infection-in-pregnant-women
#8
COMPARATIVE STUDY
Jacek Sroka, Angelina Wójcik-Fatla, Violetta Zając, Anna Sawczyn, Ewa Cisak, Jacek Karamon, Jacek Dutkiewicz, Iwona Bojar
Sera of 89 pregnant women were selected according to the results of ELFA IgM, IgG and avidity IgG, and tested with commercial tests IgM, IgG and avidity IgG Western Blot (WB) to compare the efficacy of both techniques in determining the phase of T. gondii infection. In total, 81 of 89 tested sera (91.0%) were classified as positive, both in the ELFA and WB tests for the presence of anti-Toxoplasma antibodies of class IgG, indicating a past infection, while the prevalence of anti-Toxoplasma positive reactions associated with the antibodies of class IgM indicating a recent infection was much lower - 31...
December 23, 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/27929279/pet-related-infections
#9
Michael J Day
Physicians and veterinarians have many opportunities to partner in promoting the well-being of people and their pets, especially by addressing zoonotic diseases that may be transmitted between a pet and a human family member. Common cutaneous pet-acquired zoonoses are dermatophytosis (ringworm) and sarcoptic mange (scabies), which are both readily treated. Toxoplasmosis can be acquired from exposure to cat feces, but appropriate hygienic measures can minimize the risk to pregnant women. Persons who work with animals are at increased risk of acquiring bartonellosis (e...
November 15, 2016: American Family Physician
https://www.readbyqxmd.com/read/27907933/evaluation-of-pregnant-and-postpartum-women-s-knowledge-about-toxoplasmosis-in-rio-grande-rs-brazil
#10
Lis Maurente Lehmann, Paula Costa Santos, Carlos James Scaini
Introduction Toxoplasmosis a parasitic zoonosis of global distribution, responsible for disorders during gestation can cause fetal death or congenital anomalies. Objective To evaluate the knowledge of toxoplasmosis among pregnant and postpartum women treated at the University Hospital of the city of Rio Grande, Rio Grande do Sul, Brazil. Methods This was a cross-sectional study of 100 pregnant and postpartum women at the University Hospital. Participants answered a self-administered questionnaire and gave consent for data relating to serological examinations to be abstracted from their medical records...
November 2016: Revista Brasileira de Ginecologia e Obstetrícia
https://www.readbyqxmd.com/read/27876989/seroprevalence-of-toxoplasmosis-in-diabetic-pregnant-women-in-southwestern-of-iran
#11
Jasem Saki, Shahla Shafieenia, Masoud Foroutan-Rad
To investigate anti-Toxoplasma gondii IgG and IgM antibodies in diabetic pregnant women in Ahvaz, southwest of Iran this experiment was performed. In current study the sera of 110 diabetic pregnant women as well as 110 non diabetic pregnant women referred to the hospitals affiliated with the Ahvaz Jundishapur University of Medical Sciences were assessed for anti-T. gondii IgG and IgM antibodies by ELISA and IFA methods. The ELISA assessments showed that 47 (42.7 %) and 3 (2.7 %) of diabetic women were positive for IgG and IgM antibodies, respectively...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27876900/the-common-zoonotic-protozoal-diseases-causing-abortion
#12
REVIEW
Raafat Mohamed Shaapan
Toxoplasmosis, neosporosis, sarcosporidiosis (sarcocystosis) and trypanosomiasis are the common zoonotic protozoal diseases causing abortion which caused by single-celled protozoan parasites; Toxoplasma gondii, Neospora caninum, Sarcocystis spp and Trypanosoma evansi, respectively. Toxoplasmosis is generally considered the most important disease that causing abortion of both pregnant women and different female animals throughout the world, about third of human being population had antibodies against T. gondii...
December 2016: Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology
https://www.readbyqxmd.com/read/27869937/factors-associated-to-toxoplasmosis-related-knowledge-among-pregnant-women-attending-public-health-services-in-the-municipality-of-niter%C3%A3-i-rio-de-janeiro-brazil-2013-2015
#13
Fernanda Loureiro de Moura, Patrícia Riddell Millar Goulart, Ana Paula Pereira de Moura, Thais Silva de Souza, Ana Beatriz Monteiro Fonseca, Maria Regina Reis Amendoeira
OBJECTIVE: to analyze the factors associated to toxoplasmosis-related knowledge among pregnant women attending public health services in the municipality of Niterói, Rio de Janeiro, Brazil. METHODS: this is a cross-sectional study conducted with pregnant women assisted in eight health care units; data was collected using a standardized questionnaire, from April 2013 to February 2015. RESULTS: among the 405 pregnant women interviewed, 173 (42...
July 2016: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/27861692/-gestational-and-congenital-toxoplasmosis-a-practical-approach-to-disease-notification
#14
Jaqueline Dario Capobiango, Regina Mitsuka Breganó, Fabiana Maria Ruiz Lopes Mori, Italmar Teodorico Navarro, Josemari Sawczuk de Arruda Campos, Linda Tsuiko Tatakihara, Thalita Bento Talizin, Monica Dos Santos, Tayná Rolim Galvão Pereira, Simone Garani Narciso, Edna Maria Vissoci Reiche
OBJECTIVE: to demonstrate the experience of implanting toxoplasmosis notification during pregnancy and congenital toxoplasmosis in a Sentinel Unit (SU) and describe reported cases. METHODS: this was a descriptive study of the implantation of a notification protocol using a specific notification form for suspected cases of toxoplasmosis in pregnant women and congenital toxoplasmosis in a reference center in Paraná State, Brazil, from August 2013 to August 2014. RESULTS: all 64 suspected cases were notified and case investigation was completed by the SU Epidemiology Sector; 63 received prenatal care and 51 received treatment during pregnancy; 7 of the children being clinically monitored had confirmed diagnosis of congenital toxoplasmosis...
January 2016: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/27809967/prevalence-of-toxoplasma-gondii-in-pet-dogs-in-mainland-china-a-meta-analysis
#15
Yu-Meng Gao, Huan Ding, Poppy H L Lamberton, Da-Bing Lu
Toxoplasmosis, caused by Toxoplasma gondii, is one of the most common zoonosis in the world, and can cause severe adverse consequences in pregnant women and immunosuppressed people. Throughout the world dogs are popular companion animals, however they are also important intermediate host of T. gondii and may play an instrumental role in mechanically transmitting Toxoplasma infection to humans. Therefore, an assessment of the level of T. gondii infection in pet dogs is of public health importance. To estimate the overall serological prevalence of toxoplasmosis in pet dogs in mainland China, we performed a meta-analysis using five bibliographic databases: CNKI, WanFang, VIP Chinese Journal Databases, PubMed and Google Scholar...
October 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27795962/immunity-status-of-blood-donors-regarding-toxoplasma-gondii-infection-in-a-low-income-district-of-abidjan-c%C3%A3-te-d-ivoire-west-africa
#16
Liliane Siransy, Sery Romuald Dasse, Serge Pacôme Dou Gonat, Antoinette Legbedji, Koffi N'guessan, Patricia Ama Kouacou, Richard Yeboah, Hervé Menan
Background. Toxoplasmosis is a widespread cosmopolitan anthropozoonosis, which affects more than a third of the world population. Except the modes of transmission well known, Toxoplasma gondii can be transmitted during transplantation or blood transfusion. The aim of this study is to determine the prevalence of IgG and IgM Toxoplasma gondii and to estimate the potential risk by blood products. Methods. This is a cross-sectional study on the research for Toxoplasma gondii antibodies (IgG and IgM) blood donors performed by ELISA...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27794096/high-frequency-detection-of-toxoplasma-gondii-dna-in-human-neonatal-tissue-from-libya
#17
Sameena Z H Haq, Muftah S Abushahama, Omar Gerwash, Jacqueline M Hughes, Elizabeth A Wright, Mohamed S Elmahaishi, Zhao-Rong Lun, Denise Thomasson, Geoff Hide
BACKGROUND: Toxoplasma gondii is a parasite that causes significant disease in humans. Toxoplasmosis is normally asymptomatic, unless associated with congenital transmission, or in immunocompromised people. Congenital transmission generally occurs at low frequencies. In this study, we use PCR to investigate possible congenital transmission of T. gondii during pregnancy in a cohort of mothers from Libya. METHODS: Two hundred and seventy two pregnant women (producing 276 neonates) were recruited to obtain umbilical cord tissue from their neonates at birth; DNA was extracted from that tissue and tested for T...
September 2016: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27766799/seroprevalence-of-toxoplasma-gondii-among-pregnant-women-in-northeast-india
#18
Biswajyoti Borkakoty, Dipankar Biswas, Aniruddha Jakharia, Jagadish Mahanta
OBJECTIVE: Toxoplasma gondii infection is primarily asymptomatic and one third of world's population is estimated to be infected by this protozoan parasite. This study was carried out to determine the seroprevalence of T. gondii infection among pregnant women from north east India, where data on this important parasitic infection is scanty. METHODS: A total of 1141 serum archival samples collected from antenatal clinic attendees in 2007-09, were screened for T. gondii IgG by ELISA and analyzed with their socio demographic information...
October 2016: Journal of the Association of Physicians of India
https://www.readbyqxmd.com/read/27765564/evaluation-of-the-ldbio-point-of-care-test-for-the-combined-detection-of-toxoplasmic-igg-and-igm
#19
Emmanuelle Chapey, Martine Wallon, François Peyron
The toxoplasma ICT IgG-IgM rapid diagnostic test for the simultaneous detection of specific toxoplasmic immunoglobulin (Ig) G and IgM was compared with the Architect fully automated chemiluminescence test. Four hundred sera were included, among which 248 scored negative in Architect. The cassettes were easily read with the naked eye. Diagnostic sensitivity and specificity were 97% and 96%, respectively. The test scored 8 false-positive IgG and yielded negative results in 3 sera displaying unspecific IgM in Architect...
January 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27762467/immunoglobulin-m-indirect-fluorescent-antibody-test-for-the-diagnosis-of-acute-toxoplasmosis-during-pregnancy-in-the-avidity-era-a-14-year-experience-at-the-tuscany-reference-center-for-infectious-diseases-in-pregnancy-florence-italy
#20
Michele Trotta, Beatrice Borchi, Lorenzo Zammarchi, Gaetana Sterrantino, Michela Brogi, Seble Tekle Kiros, Chiara Lorini, Guglielmo Bonaccorsi, Maria Grazia Colao, Alessandro Bartoloni
AIM: The aim of this study was to evaluate immunoglobulin M indirect-fluorescent antibody test (IgM IFAT) for the diagnosis of acute or chronic Toxoplasma infection in pregnancy. METHODS: Pregnant women with suspected acute toxoplasmosis referred to the Tuscany Reference Center for Infectious Diseases in Pregnancy during the period 1998-2012 were retrospectively enrolled. All women were tested with a panel of serological tests, including enzyme-linked immunosorbent assay for IgG avidity and IgM IFAT...
December 2016: Journal of Obstetrics and Gynaecology Research
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