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https://www.readbyqxmd.com/read/28640134/association-between-pd-l1-and-hpv-status-and-the-prognostic-value-for-hpv-treatment-in-premalignant-cervical-lesion-patients
#1
Feng Yang-Chun, Cheng Zhen-Zhen, Huang Yan-Chun, Ma Xiu-Min
The difference of PD-L1 expression between only HPV-positive patients and premalignant cervical lesion patients did not be reported in present studies. And to test the PD-L1 expression in some cervical cell lesion studies using cervical exfoliated cells sample also was ignored. Meanwhile, the PD-L1 expression as a predictive biomarker still existed controversy. So in the study, first to compare the expression of PD-L1 between only HPV-positive patients and premalignant cervical lesion patients, then to research the association between PD-L1 and HPV status, lastly to explore the possible prognostic value for HPV treatment in premalignant cervical lesion patients...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28640108/correlation-of-hla-dp-dq-polymorphisms-with-transplant-etiologies-and-prognosis-in-liver-transplant-recipients
#2
Yi Li, Qian Huang, Jiang-Tao Tang, Tian-Tian Wei, Lin Yan, Zhi-Qiang Yang, Yang-Juan Bai, Lan-Lan Wang, Yun-Ying Shi
Previous study has identified that the genetic variants in the human leukocyte antigen (HLA)-DP/DQ region were strongly associated with hepatitis B virus (HBV) infection. But their roles in liver function recovery after hepatic transplantation were still obscure. This study aimed to investigate whether HLA-DP/DQ polymorphisms were associated with post-transplant etiologies and prognosis in Chinese liver transplant recipients.A total of 144 liver transplant recipients were enrolled, which were divided into 2 groups according to the transplant etiology: HBV-related disease and non-HBV-related disease...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28638221/prevalence-of-ifnl3-rs4803217-single-nucleotide-polymorphism-and-clinical-course-of-chronic-hepatitis-c
#3
Bogna Świątek-Kościelna, Ewelina Kałużna, Ewa Strauss, Jerzy Nowak, Iwona Bereszyńska, Ewelina Gowin, Jacek Wysocki, Jolanta Rembowska, Dominika Barcińska, Iwona Mozer-Lisewska, Danuta Januszkiewicz-Lewandowska
AIM: To evaluate the association of IFNL3 (IL28B) SNP rs4803217 with severity of disease and treatment outcome in chronic hepatitis C (CHC). METHODS: The study enrolled 196 CHC Polish patients (82 women and 114 men in age 20-64) infected with hepatitis C virus (HCV) genotype 1. They were treatment naïve and qualified to pegylated interferon alpha (PEG-IFN-α) and ribavirin (RBV) therapy. The analyzed baseline parameters included: degree of inflammation, stage of fibrosis, viral load as well as alanine aminotransferase (ALT), asparagine aminotransferase (AST) and total bilirubin (TBIL)...
June 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28631817/application-of-high-resolution-melting-analysis-for-authenticity-testing-of-valuable-dendrobium-commercial-products
#4
Xiaoman Dong, Chao Jiang, Yuan Yuan, Daiyin Peng, Yuqin Luo, Yuyang Zhao, Luqi Huang
BACKGROUND: The accurate identification of botanical origin in commercial products is important to ensure food authenticity and safety for consumers. The Dendrobium species have long been commercialized as functional food supplements and herbal medicines in Asia. Three valuable Dendrobium species, namely Dendrobium officinale, D. huoshanense and D. moniliforme, are often mutually adulterated in trade products in pursuit of higher profit. RESULTS: In this paper, a rapid and reliable semi-quantitative method for identifying the botanical origin of Dendrobium products in terminal markets was developed using HRM (High-Resolution Melting) analysis with specific primer pairs to target the trnL-F region...
June 20, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28627450/impact-of-fixation-artifacts-and-threshold-selection-on-high-resolution-melting-analysis-for-kras-mutation-screening
#5
Wendy Pérez-Báez, Ethel A Garcia-Latorre, Hector Aquiles Maldonado-Martinez, Iris Coronado-Martinez, Leonardo Flores-Garcia, Lucía Taja-Chayeb
INTRODUCTION: Treatment in the metastatic colorectal cancer has expanded with monoclonal antibodies targeting Epidermal Growth Factor Receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) tissues are based on Real-time PCR. Among them, high resolution melting analysis (HRMA), is a simple, fast, highly sensitive, specific and cost-effective method, proposed as adjunct for KRAS mutation detection...
June 13, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28620610/molecular-detection-of-tick-borne-pathogen-diversities-in-ticks-from-livestock-and-reptiles-along-the-shores-and-adjacent-islands-of-lake-victoria-and-lake-baringo-kenya
#6
David Omondi, Daniel K Masiga, Burtram C Fielding, Edward Kariuki, Yvonne Ukamaka Ajamma, Micky M Mwamuye, Daniel O Ouso, Jandouwe Villinger
Although diverse tick-borne pathogens (TBPs) are endemic to East Africa, with recognized impact on human and livestock health, their diversity and specific interactions with tick and vertebrate host species remain poorly understood in the region. In particular, the role of reptiles in TBP epidemiology remains unknown, despite having been implicated with TBPs of livestock among exported tortoises and lizards. Understanding TBP ecologies, and the potential role of common reptiles, is critical for the development of targeted transmission control strategies for these neglected tropical disease agents...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28620232/real-time-monitoring-of-laser-powder-bed-fusion-process-using-high-speed-x-ray-imaging-and-diffraction
#7
Cang Zhao, Kamel Fezzaa, Ross W Cunningham, Haidan Wen, Francesco De Carlo, Lianyi Chen, Anthony D Rollett, Tao Sun
We employ the high-speed synchrotron hard X-ray imaging and diffraction techniques to monitor the laser powder bed fusion (LPBF) process of Ti-6Al-4V in situ and in real time. We demonstrate that many scientifically and technologically significant phenomena in LPBF, including melt pool dynamics, powder ejection, rapid solidification, and phase transformation, can be probed with unprecedented spatial and temporal resolutions. In particular, the keyhole pore formation is experimentally revealed with high spatial and temporal resolutions...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28616916/-relationship-between-lep-g2548a-polymorphism-and-cholesterol-gallstone
#8
Jing Chen, Jie He, Jiang-Hui Chen, Zhen-Bin Lai, Qi-Jun Cheng, Xiao-Shan Yu, Hui-Fen Liu, Yan-Hua Su, Ben-Hua Zhao
OBJECTIVES: To determine the associations of single nucleotide polymorphism (SNP) in leptin (LEP) genes and environmental factors with cholesterol gallstone in southeast Han populations. METHODS: A 1:2 matched case-control study was conducted involving 200 patients with cholesterol gallstone. Genotyping of the SNP was examined on the LightCycler480 PCR platform using in-house high resolution melting (HRM) approaches. Detection correctness was validated through direct sequencing...
May 2017: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28616555/mtrr-rs326119-polymorphism-is-associated-with-plasma-concentrations-of-homocysteine-and-cobalamin-but-not-with-congenital-heart-disease-or-coronary-atherosclerosis-in-brazilian-patients
#9
Melanie Horita, Carolina Tosin Bueno, Andrea R Horimoto, Pedro A Lemos, Antonio A Morandini-Filho, Jose E Krieger, Paulo C J L Santos, Alexandre C Pereira
BACKGROUND: Differences in the distribution of the MTRR rs326119 polymorphism (c.56 + 781 A > C) between patients with congenital heart disease (CHD) and controls have been described in Chinese individuals. The association is thought to be due to deregulation of homocysteine-cobalamin pathways. This has not been replicated in other populations. The primary objective of this study was to assess the influence of the MTRR rs326119 polymorphism on biochemical parameters of vitamin B12 metabolism, coronary lesions, and congenital heart disease in Brazilian subjects...
March 2017: IJC Heart & Vasculature
https://www.readbyqxmd.com/read/28616554/alpha2a-adrenergic-receptor-and-enos-genetic-polymorphisms-are-associated-with-exercise-muscle-vasodilatation-in-apparently-healthy-individuals
#10
Rafael Amorim Belo Nunes, Lucia Pereira Barroso, Alexandre da Costa Pereira, Maria Urbana Pinto Brandão Rondon, Carlos Eduardo Negrão, José Eduardo Krieger, Alfredo José Mansur
PURPOSE: Muscle vasodilatation during exercise has been associated with cardiovascular health and may be influenced by genetic variability. The purpose of this study was to evaluate functional genetic polymorphisms of physiologic pathways related to the regulation of the cardiovascular function (alpha-adrenergic receptors, endothelial nitric oxide synthase and bradykinin B2 receptor) and exercise muscle vasodilatation in apparently healthy men and women. METHODS: We enrolled 689 individuals without established cardiovascular disease that had attended a check-up program...
December 2016: IJC Heart & Vasculature
https://www.readbyqxmd.com/read/28615016/ex-vivo-susceptibility-and-genotyping-of-plasmodium-falciparum-isolates-from-pikine-senegal
#11
Aminata Mbaye, Amy Gaye, Baba Dieye, Yaye D Ndiaye, Amy K Bei, Muna Affara, Awa B Deme, Mamadou S Yade, Khadim Diongue, Ibrahima M Ndiaye, Tolla Ndiaye, Mouhamed Sy, Ngayo Sy, Ousmane Koita, Donald J Krogstad, Sarah Volkman, Davis Nwakanma, Daouda Ndiaye
BACKGROUND: The monitoring of Plasmodium falciparum sensitivity to anti-malarial drugs is a necessity for effective case management of malaria. This species is characterized by a strong resistance to anti-malarial drugs. In Senegal, the first cases of chloroquine resistance were reported in the Dakar region in 1988 with nearly 7% population prevalence, reaching 47% by 1990. It is in this context that sulfadoxine-pyrimethamine temporarily replaced chloroquine as first line treatment in 2003, pending the introduction of artemisinin-based combination therapy in 2006...
June 14, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28603011/a-comprehensive-spectroscopic-study-of-the-polymorphs-of-diflunisal-and-their-phase-transformations
#12
Anuradha R Pallipurath, Francesco Civati, Juraj Sibik, Clare Crowley, J Axel Zeitler, Patrick McArdle, Andrea Erxleben
Understanding phase transitions in pharmaceutical materials is of vital importance for drug manufacturing, processing and storage. In this paper we have carried out comprehensive high-resolution spectroscopic studies on the polymorphs of the non-steroidal anti-inflammatory drug diflunisal that has four known polymorphs, forms I-IV (FI-FIV), three of which have known crystal structures. Phase transformations during milling, heating, melt-quenching and exposure to high relative humidity were investigated using Raman and terahertz spectroscopy in combination with differential scanning calorimetry and X-ray powder diffraction...
June 8, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28592231/high-burden-of-complicated-skin-and-soft-tissue-infections-in-the-indigenous-population-of-central-australia-due-to-dominant-panton-valentine-leucocidin-clones-st93-mrsa-and-cc121-mssa
#13
Susan A J Harch, Eleanor MacMorran, Steven Y C Tong, Deborah C Holt, Judith Wilson, Eugene Athan, Saliya Hewagama
BACKGROUND: Superficial skin and soft tissue infections (SSTIs) are common among the Indigenous population of the desert regions of Central Australia. However, the overall burden of disease and molecular epidemiology of Staphylococcus aureus complicated SSTIs has yet to be described in this unique population. METHODS: Alice Springs Hospital (ASH) admission data was interrogated to establish the population incidence of SSTIs. A prospective observational study was conducted on a subset of S...
June 7, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28591744/multiple-gene-specific-dna-methylation-in-blood-leukocytes-and-colorectal-cancer-risk-a-case-control-study-in-china
#14
Yupeng Liu, Yibaina Wang, Fulan Hu, Hongru Sun, Zuoming Zhang, Xuan Wang, Xiang Luo, Lin Zhu, Rong Huang, Yan Li, Guangxiao Li, Xia Li, Shangqun Lin, Fan Wang, Yanhong Liu, Jiesheng Rong, Huiping Yuan, Yashuang Zhao
The relationship between gene-specific DNA methylation in peripheral blood leukocytes and colorectal cancer (CRC) susceptibility is unclear. In this case-control study, the methylation status of a panel of 10 CRC-related genes in 428 CRC cases and 428 cancer-free controls were detected with methylation-sensitive high-resolution melting analysis. We calculated a weighted methylation risk score (MRS) that comprehensively combined the methylation status of the panel of 10 genes and found that the MRS_10 was significantly associated with CRC risk...
May 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28578596/high-resolution-melting-analysis-of-gyra-codon-84-and-grla-codon-80-mutations-conferring-resistance-to-fluoroquinolones-in-staphylococcus-pseudintermedius-isolates-from-canine-clinical-samples
#15
Monica Loiacono, Piera Anna Martino, Francesca Albonico, Francesca Dell'Orco, Manuela Ferretti, Sergio Zanzani, Michele Mortarino
Staphylococcus pseudintermedius is an opportunistic pathogen of dogs and cats. A high-resolution melting analysis (HRMA) protocol was designed and tested on 42 clinical isolates with known fluoroquinolone (FQ) susceptibility and gyrA codon 84 and grlA codon 80 mutation status. The HRMA approach was able to discriminate between FQ-sensitive and FQ-resistant strains and confirmed previous reports that the main mutation site associated with FQ resistance in S. pseudintermedius is located at position 251 (Ser84Leu) of gyrA...
June 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28576321/quantification-of-gene-specific-dna-methylation-in-oesophageal-cancer-via-electrochemistry
#16
Md Hakimul Haque, Vinod Gopalan, Md Nazmul Islam, Mostafa Kamal Masud, Ripon Bhattacharjee, Md Shahriar Al Hossain, Nam-Trung Nguyen, Alfred K Lam, Muhammad J A Shiddiky
Development of simple and inexpensive method for the analysis of gene-specific DNA methylation is important for the diagnosis and prognosis of patients with cancer. Herein, we report a relatively simple and inexpensive electrochemical method for the sensitive and selective detection of gene-specific DNA methylation in oesophageal cancer. The underlying principle of the method relies on the affinity interaction between DNA bases and unmodified gold electrode. Since the affinity trend of DNA bases towards the gold surface follows as adenine (A) > cytosine (C) > guanine (G)> thymine (T), a relatively larger amount of bisulfite-treated adenine-enriched unmethylated DNA adsorbs on the screen-printed gold electrodes (SPE-Au) in comparison to the guanine-enriched methylated sample...
July 11, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28574180/topical-equol-preparation-improves-structural-and-molecular-skin-parameters
#17
U Magnet, Chr Urbanek, D Gaisberger, E Tomeva, E Dum, A Pointner, A G Haslberger
OBJECTIVE: Equol has been shown to improve skin health and regeneration, due to its antioxidative, phytoestrogenic and epigenetic characteristics. The effects of a topical intervention on skin structure, telomere length and epigenetic markers in skin cells were analysed. METHODS: 64 participants were divided in four groups and three of them treated topically with: emulsion with Equol powder (Isoflavandiol-E-55-RS(®) ); emulsion with microencapsulated Equol (Vesisorb(®) Isoflavandiol-E-55-RS(®) ) and an emulsion with lecithin (Vesisorb(®) placebo)...
June 2, 2017: International Journal of Cosmetic Science
https://www.readbyqxmd.com/read/28570560/qpcr-high-resolution-melt-analysis-for-drug-susceptibility-testing-of-mycobacterium-leprae-directly-from-clinical-specimens-of-leprosy-patients
#18
Sergio Araujo, Luiz Ricardo Goulart, Richard W Truman, Isabela Maria B Goulart, Varalakshmi Vissa, Wei Li, Masanori Matsuoka, Philip Suffys, Amanda B Fontes, Patricia S Rosa, David M Scollard, Diana L Williams
BACKGROUND: Real-Time PCR-High Resolution Melting (qPCR-HRM) analysis has been recently described for rapid drug susceptibility testing (DST) of Mycobacterium leprae. The purpose of the current study was to further evaluate the validity, reliability, and accuracy of this assay for M. leprae DST in clinical specimens. METHODOLOGY/PRINCIPAL FINDINGS: The specificity and sensitivity for determining the presence and susceptibility of M. leprae to dapsone based on the folP1 drug resistance determining region (DRDR), rifampin (rpoB DRDR) and ofloxacin (gyrA DRDR) was evaluated using 211 clinical specimens from leprosy patients, including 156 multibacillary (MB) and 55 paucibacillary (PB) cases...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28569339/high-resolution-melting-as-a-rapid-reliable-accurate-and-cost-effective-emerging-tool-for-genotyping-pathogenic-bacteria-and-enhancing-molecular-epidemiological-surveillance-a-comprehensive-review-of-the-literature
#19
M Tamburro, G Ripabelli
INTRODUCTION: Rapid, reliable and accurate molecular typing methods are essential for outbreaks detection and infectious diseases control, for monitoring the evolution and dynamics of microbial populations, and for effective epidemiological surveillance. The introduction of a novel method based on the analysis of melting temperature of amplified products, known as High Resolution Melting (HRM) since 2002, has found applications in epidemiological studies, either for identification of bacterial species or molecular typing, as well as an extensive and increasing use in many research fields...
July 2017: Annali di Igiene: Medicina Preventiva e di Comunità
https://www.readbyqxmd.com/read/28552715/influence-of-scarb1-gene-snps-on-serum-lipid-levels-and-susceptibility-to-coronary-heart-disease-and-cerebral-infarction-in-a-chinese-population
#20
Ting-Ting Zeng, Di-Jiao Tang, Yuan-Xin Ye, Jun Su, Hong Jiang
The SCARB1 gene encodes human scavenger receptor class B type I (SR-BI), the primary receptor for high-density lipoprotein (HDL)- cholesteryl ester uptake, and polymorphisms in this gene may influence SR-BI protein expression and serum lipid levels, modulating susceptibility to coronary heart disease (CHD) and cerebral infarction (CI). Therefore, we investigated the association between singlenucleotide polymorphisms (SNPs) in the SCARB1 gene and serum lipid levels as well as risk of CHD and CI in the Chinese Han population...
May 24, 2017: Gene
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