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https://www.readbyqxmd.com/read/28819966/characterization-of-nanopipet-supported-ities-tips-for-scanning-electrochemical-microscopy-of-single-solid-state-nanopores
#1
Ran Chen, Ryan James Balla, Alex Lima, Shigeru Amemiya
Nanoscale scanning electrochemical microscopy (SECM) is a powerful scanning probe technique that enables high-resolution imaging of chemical processes at single nanometer-sized objects. However, it has been a challenging task to quantitatively understand nanoscale SECM images, which requires to accurately characterize the size and geometry of nanoelectrode tips. Herein, we address this challenge through transmission electron microscopy (TEM) of quartz nanopipets for SECM imaging of single solid-state nanopores by using nanopipet-supported interfaces between two immiscible electrolyte solutions (ITIES) as tips...
August 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28818830/high-speed-melting-analysis-the-effect-of-melting-rate-on-small-amplicon-microfluidic-genotyping
#2
Robert J Pryor, Joseph T Myrick, Robert A Palais, Scott O Sundberg, Jeanette Y Paek, Carl T Wittwer, Ivor T Knight
BACKGROUND: High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS: Using a microfluidic platform for serial PCR and melting analysis, 4 targets containing single nucleotide variants were amplified and then melted at different rates over a 250-fold range from 0.13 to 32ü°C/s. Genotypes (n = 1728) were determined manually by visual inspection after background removal, normalization, and conversion to negative derivative plots...
August 17, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28808709/phase-diagram-and-structural-evolution-of-tin-indium-sn-in-nanosolder-particles-from-a-non-equilibrium-state-to-an-equilibrium-state
#3
Yang Shu, Teiichi Ando, Qiyue Yin, Guangwen Zhou, Zhiyong Gu
A binary system of tin/indium (Sn/In) in the form of nanoparticles was investigated for phase transitions and structural evolution at different temperatures and compositions. The Sn/In nanosolder particles in the composition range of 24-72 wt% In were synthesized by a surfactant-assisted chemical reduction method under ambient conditions. The morphology and microstructure of the as-synthesized nanoparticles were analyzed by scanning electron microscopy (SEM), high resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED) and X-ray diffraction (XRD)...
August 15, 2017: Nanoscale
https://www.readbyqxmd.com/read/28802406/real-time-pcr-followed-by-high-resolution-melting-curve-analysis-a-rapid-and-pragmatic-approach-for-screening-of-multidrug-resistant-extrapulmonary-tuberculosis
#4
Kusum Sharma, Megha Sharma, Shreya Singh, Manish Modi, Aman Sharma, Pallab Ray, Subhash Varma
INTRODUCTION: Multidrug resistance (MDR) in extrapulmonary tuberculosis (EPTB) is a diagnostic challenge in an endemic country like India. Timely detection of MDR-TB can contribute to a better patient outcome. OBJECTIVE: To perform real-time PCR (qPCR) using rpoB, mpb64 and IS6110 gene on a variety of EPTB samples and to compare the performance of different gene targets. All qPCR positive samples were subjected to high resolution melt-curve analysis (HRM analysis) for rpoB and katG gene to evaluate its potential for MDR screening among different sample types...
September 2017: Tuberculosis
https://www.readbyqxmd.com/read/28799826/multiplex-assay-for-identifying-animal-species-found-in-the-tibetan-area-using-the-mitochondrial-12s-rrna-gene
#5
Jung Nam Lee, MingFeng Jiang, YongLi Wen, ShiLin Li, GuoRong Yuan
Southwestern China has an area with unique natural conditions located in alpine regions at altitudes from 2000 to 5000 m; this area is referred to as the Qinghai-Tibetan plateau (QTP). Unique animals, such as yaks (Bos grunniens), are found extensively on the plateau of Southwestern China due to its unique environment. In recent years, the prevalence of fake meat products such as fake jerky has increased in this area. This research was conducted as an attempt to develop a reliable multiplex polymerase chain reaction (mPCR) detection method for identifying nine animal species found in QTP...
August 11, 2017: Animal Biotechnology
https://www.readbyqxmd.com/read/28799177/racemic-and-enantiopure-forms-of-3-ethyl-3-phenylpyrrolidin-2-one-adopt-very-different-crystal-structures
#6
Arcadius V Krivoshein, Sergey V Lindeman, Tatiana V Timofeeva, Victor N Khrustalev
3-Ethyl-3-phenylpyrrolidin-2-one (EPP) is an experimental anticonvulsant based on the newly proposed α-substituted amide group pharmacophore. These compounds show robust activity in animal models of drug-resistant epilepsy and are thus promising for clinical development. In order to understand pharmaceutically relevant properties of such compounds, we are conducting an extensive investigation of their structures in the solid state. In this article, we report chiral high-performance liquid chromatography (HPLC) separation, determination of absolute configuration of enantiomers, and crystal structures of EPP...
August 11, 2017: Chirality
https://www.readbyqxmd.com/read/28795223/high-resolution-melting-pcr-assay-applicable-for-diagnostics-and-screening-studies-allowing-detection-and-differentiation-of-several-babesia-spp-infecting-humans-and-animals
#7
Wioletta Rozej-Bielicka, Aleksander Masny, Elzbieta Golab
The goal of the study was to design a single tube PCR test for detection and differentiation of Babesia species in DNA samples obtained from diverse biological materials. A multiplex, single tube PCR test was designed for amplification of approximately 400 bp region of the Babesia 18S rRNA gene. Universal primers were designed to match DNA of multiple Babesia spp. and to have low levels of similarity to DNA sequences of other intracellular protozoa and Babesia hosts. The PCR products amplified from Babesia DNA isolated from human, dog, rodent, deer, and tick samples were subjected to high-resolution melting analysis for Babesia species identification...
August 10, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28794126/a-refined-method-to-study-gene-dosage-changes-in-vitro-using-crispr-cas9
#8
Teresa P Raposo, Henry O Ebili, Mohammad Ilyas
AIMS: Gene dosage can have a major impact on cell biology, although, hitherto, it has been difficult to study using in vitro models. We sought to refine and accelerate the development of 'gene dosage' models through using CRISPR/Cas9 (a gene editing technology) for sequential knockout of gene alleles. METHODS: Our method involved (1) using Cas9 nuclease mRNA rather than expression plasmids, (2) using a fluorescently labelled FAM-6 tracr complexed with guide RNA and (3) using high-resolution melting (HRM) analysis to screen for mutations...
August 9, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28791595/high-resolution-melting-analysis-for-prenatal-diagnosis-of-beta-thalassemia-in-northern-thailand
#9
Pimlak Charoenkwan, Supatra Sirichotiyakul, Arunee Phusua, Sudjai Suanta, Kanda Fanhchaksai, Rattika Sae-Tung, Torpong Sanguansermsri
High-resolution melting (HRM) analysis is a rapid mutation analysis which assesses the pattern of reduction of fluorescence signal after subjecting the amplified PCR product with saturated fluorescence dye to an increasing temperature. We used HRM analysis for prenatal diagnosis of beta-thalassemia disease in northern Thailand. Five PCR-HRM protocols were used to detect point mutations in five different segments of the beta-globin gene, and one protocol to detect the 3.4 kb beta-globin deletion. We sought to characterize the mutations in carriers and to enable prenatal diagnosis in 126 couples at risk of having a fetus with beta-thalassemia disease...
August 8, 2017: International Journal of Hematology
https://www.readbyqxmd.com/read/28783496/the-transport-of-polycyclic-aromatic-hydrocarbons-during-rainfall-and-snowmelt-in-contrasting-landscapes
#10
Abha Parajulee, Ying Duan Lei, Ajitha Kananathalingam, David S McLagan, Carl P J Mitchell, Frank Wania
Though it has been established that stream concentrations of polycyclic aromatic hydrocarbons (PAHs) in urban watersheds can be much greater than those in less developed watersheds, knowledge of transport mechanisms is lacking, particularly in temperate, Northern climates with seasonal snow packs. We combine high-resolution stream water sampling with air, suspended solid and stream flow monitoring to investigate the source to stream transport of PAHs during rainfall and snowmelt in paired watersheds with contrasting land use...
July 31, 2017: Water Research
https://www.readbyqxmd.com/read/28777845/-rapid-detection-of-hot-spot-mutations-of-fgfr3-gene-with-pcr-high-resolution-melting-assay
#11
Shan Li, Han Wang, Hua Su, Jinsong Gao, Xiuli Zhao
OBJECTIVE: To identify the causative mutations in five individuals affected with dyschondroplasia and develop an efficient procedure for detecting hot spot mutations of the FGFR3 gene. METHODS: Genomic DNA was extracted from peripheral blood samples with a standard phenol/chloroform method. PCR-Sanger sequencing was used to analyze the causative mutations in the five probands. PCR-high resolution melting (HRM) was developed to detect the identified mutations. RESULTS: A c...
August 10, 2017: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/28777095/pcsk-9-gain-of-function-mutations-r496w-and-d374y-and-clinical-cardiovascular-characteristics-in-a-cohort-of-turkish-patients-with-familial-hypercholesterolemia
#12
Esra Kaya, Meral Kayıkçıoğlu, Aslı Tetik Vardarlı, Zuhal Eroğlu, Serdar Payzın, Levent Can
OBJECTIVE: The molecular basis of the mutations in the PCSK9 gene that produces familial hypercholesterolemia (FH) in the Turkish population is unknown. This study was conducted to determine the presence of four different PCSK9 gain-of-function (GOF) mutations (F216L, R496W, S127R, and D374Y) in a group of patients with FH. METHODS: A total of 80 consecutive patients with FH (mean age: 56±11 years; mean maximum LDL cholesterol: 251±76 mg/dL) were included in the study...
August 2, 2017: Anatolian Journal of Cardiology
https://www.readbyqxmd.com/read/28774095/an-investigation-of-sintering-parameters-on-titanium-powder-for-electron-beam-melting-processing-optimization
#13
Philipp Drescher, Mohamed Sarhan, Hermann Seitz
Selective electron beam melting (SEBM) is a relatively new additive manufacturing technology for metallic materials. Specific to this technology is the sintering of the metal powder prior to the melting process. The sintering process has disadvantages for post-processing. The post-processing of parts produced by SEBM typically involves the removal of semi-sintered powder through the use of a powder blasting system. Furthermore, the sintering of large areas before melting decreases productivity. Current investigations are aimed at improving the sintering process in order to achieve better productivity, geometric accuracy, and resolution...
December 1, 2016: Materials
https://www.readbyqxmd.com/read/28769035/spiraling-pathways-of-global-deep-waters-to-the-surface-of-the-southern-ocean
#14
Veronica Tamsitt, Henri F Drake, Adele K Morrison, Lynne D Talley, Carolina O Dufour, Alison R Gray, Stephen M Griffies, Matthew R Mazloff, Jorge L Sarmiento, Jinbo Wang, Wilbert Weijer
Upwelling of global deep waters to the sea surface in the Southern Ocean closes the global overturning circulation and is fundamentally important for oceanic uptake of carbon and heat, nutrient resupply for sustaining oceanic biological production, and the melt rate of ice shelves. However, the exact pathways and role of topography in Southern Ocean upwelling remain largely unknown. Here we show detailed upwelling pathways in three dimensions, using hydrographic observations and particle tracking in high-resolution models...
August 2, 2017: Nature Communications
https://www.readbyqxmd.com/read/28764056/species-identification-in-meat-products-a-new-screening-method-based-on-high-resolution-melting-analysis-of-cyt-b-gene
#15
A Lopez-Oceja, C Nuñez, M Baeta, D Gamarra, M M de Pancorbo
Meat adulteration by substitution with lower value products and/or mislabeling involves economic, health, quality and socio-religious issues. Therefore, identification and traceability of meat species has become an important subject to detect possible fraudulent practices. In the present study the development of a high resolution melt (HRM) screening method for the identification of eight common meat species is reported. Samples from Bos taurus, Ovis aries, Sus scrofa domestica, Equus caballus, Oryctolagus cuniculus, Gallus gallus domesticus, Meleagris gallopavo and Coturnix coturnix were analyzed through the amplification of a 148 bp fragment from the cyt b gene with a universal primer pair in HRM analyses...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28761481/comparison-of-three-pcr-based-methods-for-simplicity-and-cost-effectiveness-identification-of-cutaneous-leishmaniasis-due-to-leishmania-tropica
#16
Mohammad Ali Mohammadi, Mehdi Bamorovat, Majid Fasihi Harandi, Tayyebeh Karimi, Iraj Sharifi, Mohammad Reza Aflatoonian
BACKGROUND: To compare three molecular methods, PCR-RFLP for internal transcribed spacer, PCR sequencing and high resolution melting analysis shown reliable sensitivity and specificity for detecting Leishmania tropica as a model for cutaneous leishmaniasis (CL) as the perspective overview for scientific and economic approaches. METHODS: This study was carried out between 2015 and 2016 in Leishmaniasis Research Center in Kerman University of Medical Sciences, Kerman, Iran...
April 2017: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28758965/identification-and-quantification-of-celery-allergens-using-fiber-optic-surface-plasmon-resonance-pcr
#17
Devin Daems, Bernd Peeters, Filip Delport, Tony Remans, Jeroen Lammertyn, Dragana Spasic
Abstract: Accurate identification and quantification of allergens is key in healthcare, biotechnology and food quality and safety. Celery (Apium graveolens) is one of the most important elicitors of food allergic reactions in Europe. Currently, the golden standards to identify, quantify and discriminate celery in a biological sample are immunoassays and two-step molecular detection assays in which quantitative PCR (qPCR) is followed by a high-resolution melting analysis (HRM). In order to provide a DNA-based, rapid and simple detection method suitable for one-step quantification, a fiber optic PCR melting assay (FO-PCR-MA) was developed to determine different concentrations of celery DNA (1 pM-0...
July 31, 2017: Sensors
https://www.readbyqxmd.com/read/28745649/high-resolution-melting-pcr-for-complement-receptor-1-length-polymorphism-genotyping-an-innovative-tool-for-alzheimer-s-disease-gene-susceptibility-assessment
#18
Aymric Kisserli, Thierry Tabary, Jacques Henri Max Cohen, Valérie Duret, Rachid Mahmoudi
Complement receptor 1 (CR1), a transmembrane glycoprotein that plays a key role in the innate immune system, is expressed on many cell types, but especially on red blood cells (RBCs). As a receptor for the complement components C3b and C4b, CR1 regulates the activation of the complement cascade and promotes the phagocytosis of immune complexes and cellular debris, as well as the amyloid-beta (Aβ) peptide in Alzheimer's disease (AD). Several studies have confirmed AD-associated single nucleotide polymorphisms (SNPs), as well as a copy-number variation (CNV) in the CR1 gene...
July 18, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28740450/single-nucleotide-polymorphisms-in-genes-macc1-rad18-mmp7-and-sdf-1a-as-prognostic-factors-in-resectable-colorectal-cancer
#19
Matej Horvat, Uros Potocnik, Katja Repnik, Rajko Kavalar, Vesna Zadnik, Stojan Potrc, Borut Stabuc
BACKGROUND: Colorectal cancer (CRC) represents one of the most common malignancies worldwide. Research has indicated that functional gene changes such as single nucleotide polymorphism (SNP) influence carcinogenesis and metastasis and might have an influence on disease relapse. The aim of our study was to evaluate the role of SNPs in selected genes as prognostic markers in resectable CRC. PATIENTS AND METHODS: In total, 163 consecutive patients treated surgically for CRC of stages I, II and III at the University Medical Centre in Maribor in 2007 and 2008 were investigated...
June 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/28734895/a-sensitive-and-convenient-method-for-clinical-detection-of-non-syndromic-hearing-loss-associated-common-mutations
#20
Er-Feng Yuan, Wei Xia, Jing-Tao Huang, Ling Hu, Xing Liao, Xiang Dai, Song-Mei Liu
BACKGROUND: The majority of non-syndromic hearing loss (NSHL) patients result from causative mutations in GJB2, SLC26A4 and mitochondrial 12S rRNA genes. Accurate detection of these genetic mutations is increasingly recognized for its clinical significance to reduce incidence and guide individual treatment of NSHL. Current methods for clinical practice are labor intensive, expensive or of low sensitivity. METHODS: Genomic DNA from 7 newborns not passing the hearing screening and 94 newborns passing the hearing screening were analyzed for the common mutations using high resolution melting analysis (HRMA) and Sanger sequencing...
July 19, 2017: Gene
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