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Yunchun Zhou, Qian Liu, Xuping Sun, Rongmei Kong
The determination of specific nucleic acid sequences is key in identifying disease-causing pathogens and genetic diseases. In this paper we report the utilization of Fe-nitrilotriacetic acid coordination polymer nanowires as an effective nanoquencher for fluorescence-enhanced nucleic acid detection. The detection is fast and the whole process can be completed within 15 min. This nanosensor shows a low detection limit of 0.2 nM with selectivity down to single-base mismatch. This work provides us with an attractive sensing platform for applications...
January 12, 2017: Nanotechnology
Farhad Akhgari, Naser Samadi, Khalil Farhadi
Here a simple and sensitive fluorescent assay for detecting Cefixime based on inner filter effect (IFE) has been proven, which is conceptually different from the previously reported CEF fluorescent assays. In this sensing platform, fluorescent carbon dots (CDs) were prepared by one-pot synthesis and was directly used as fluorophore in IFE. The method is based on the complexation reaction between cefixime and palladium ion in the presence of acidic buffer solution (pH 4). The Pd(II)-CEFcomplex was capable of functioning as a powerful absorber in IFE to influence the excitation of fluorophore (CDs)...
January 11, 2017: Journal of Fluorescence
Anran Gao, Xun Yang, Jing Tong, Lin Zhou, Yuelin Wang, Jianlong Zhao, Hongju Mao, Tie Li
In this work, a real-time assay for highly sensitive, label-free, multiplexed electrical detection of lung cancer biomarkers was developed by using silicon nanowire field-effect (SiNW-FET) devices. Highly responsive SiNW arrays were fabricated using a CMOS-compatible anisotropic self-stop etching technique with mass reproducibility and low cost character. The SiNW nanosensor was integrated with PDMS microfluidic device, which allows rapid analyte delivery, makes the analysis to be conducted using exceedingly small samples and enables potential multiplexed detection...
January 3, 2017: Biosensors & Bioelectronics
Jian Zhang, Lifang He, Peirong Chen, Chao Tian, Jianping Wang, Bianhua Liu, Changlong Jiang, Zhongping Zhang
Chemical sensing for the convenient detection of trace aqueous fluoride ions (F(-)) has been widely explored with the use of various sensing materials and techniques. It still remains a challenge to achieve ultrasensitive but simple, rapid, and inexpensive detection of F(-) for environmental monitoring and protection. Here we reported a novel surface-enhanced Raman scattering (SERS) nanosensor, fluorescein phenylboronic acid covalently linked to 1,4-dimercapto-2,3-butanediol modified Au@Ag NPs by a cyclic boronate ester (Flu-PBA-Diol-Au@Ag NPs), for the rapid and ultrasensitive detection of F(-)...
January 10, 2017: Nanoscale
Monireh Bakhshpour, Erdoğan Özgür, Nilay Bereli, Adil Denizli
Detection of protein C (PC) in human serum was performed by quartz crystal microbalance (QCM) based on molecular imprinting technique (MIP). The high-resolution and mass-sensitive QCM based sensor was integrated with high sensitivity and selectivity of the MIP technique. The PC microcontact imprinted (PC-μCIP) nanofilm was prepared on the glass surface. Then, the PC-μCIP/QCM sensor was prepared with 2-hydroxyethyl methacrylate (HEMA), ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl l-histidine methylester (MAH) as the functional monomer with copper(II) ions...
December 15, 2016: Colloids and Surfaces. B, Biointerfaces
Meng Jin, Zhao-Li Mou, Rui-Ling Zhang, Si-Si Liang, Zhi-Qi Zhang
The development of a simple and accurate quantitative method for the determination of 6-mercaptopurine (6-MP) is of great importance because of its serious side effects. Ratiometric fluorescence (RF) sensors are not subject to interference from environmental factors, and exhibit enhanced precision and accuracy. Therefore, a novel RF sensor for the selective detection of 6-MP was developed based on a dual-emission nanosensor. The nanosensor was fabricated by combining a blue-emission metal-organic framework (MOF) NH2-MIL-53(Al) (λem=425nm) with green-emission 3-mercaptopropionic acid-capped CdTe quantum dots (MPA-CdTe QDs) (λem=528nm) under a single excitation wavelength (335nm)...
December 9, 2016: Biosensors & Bioelectronics
Zhi Yong Bao, Wei Zhang, Yong-Liang Zhang, Jijun He, Jiyan Dai, Chi-Tung Yeung, Ga-Lai Law, Dang Yuan Lei
Detailed understanding of the interaction between a chiral molecule and a noble metal surface is essential to rationalize and advance interfacial self-assembly of amino acids and metal-mediated anchoring of proteins. Here we demonstrate that individual Au@Ag core-shell nanocuboids can serve as a plasmonic reporter of an extended helical network formed among chemisorbed cysteine molecules, through generating an interband absorption enhanced, Ag-surface-exclusive circular dichroism (CD) band in the UV region...
December 22, 2016: Angewandte Chemie
H Tavassoli Larijani, M Jahanshahi, M Darvish Ganji, M H Kiani
In the present work, the adsorption of glycine amino acid and its zwitterionic form onto three different hexagonal sheets, namely graphene, boron-nitride (h-BN) and silicon carbide (h-SiC), has been investigated within the framework of Density Functional Theory (DFT) calculations. The energetics and geometrical parameters of the considered systems have been explored at the GGA-PBE level in combination with Grimme's empirical dispersion corrections with Becke-Johnson damping, the DFT-D3(BJ) method. Based on the obtained results, we found that both the glycine molecule and its zwitterionic conformation tend to be chemisorbed onto the surface of h-SiC (Eads ranges from -1...
December 22, 2016: Physical Chemistry Chemical Physics: PCCP
Tao Xie, Chao Jing, Yi-Tao Long
Plasmonic nanoparticles, especially Au and Ag, exhibit unique and tuneable optical properties due to their localized surface plasmon resonance, which have been widely applied in cell imaging, biocatalysis and biosensing. Notably, dark-field microscopy enables the observation of the scattering spectra of single nanoparticles and therefore has been intensively used in the construction of various nanosensors. In this mini review, we will briefly introduce the recent applications of plasmonic nanoparticles based on dark-field microscopy...
December 22, 2016: Analyst
Paolo Pierobon, Ana-Maria Lennon-Duménil
Using an exquisite cell imaging approach based on DNA nanosensors, Spillane and Tolar (2016. J. Cell Biol. explore how the physical properties of antigen-presenting cell surfaces affect how B cells internalize surface-tethered antigens. Soft and flexible surfaces promote mechanical force-mediated antigen extraction, whereas stiff surfaces lead to enzyme-mediated antigen release before subsequent internalization.
January 2, 2017: Journal of Cell Biology
B Rezaei, M Shahshahanipour, Ali A Ensafi
In the present research, a sensitive biosensing method was proposed for the detection of trace amounts of ascorbic acid (AA). Herein, colloidal silver nanoparticles (SNPs) were successfully in-situ produced by chemical reduction of silver ion in the presence of AA, as a reducing agent. The one-pot in-situ produced silver nanoparticles were characterized by UV-vis, dynamic light scattering (DLS), zeta potential and transmission electron microscopic (TEM). SNPs act as a strong fluorescence quencher for the CdTe quantum dots via an inner filter effect (IFE)...
February 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Haowen Huang, Lanfang Liu, Lingyang Zhang, Qian Zhao, Yuan Zhou, Shishan Yuan, Zilong Tang, Xuanyong Liu
Ethylene diamine tetraacetic acid (EDTA) is such a powerful chelating agent that it may form stable complexes with most metal ions, which has wide applications in industry, agriculture, environment, and pharmaceutical technology. Recently, EDTA was found to enhance the photocatalytic property of some materials. Inspired by this fact of EDTA in the photocatalytic role, we further investigated the photocatalytic property of EDTA and found much the same as that of natural horseradish peroxidase (HRP). This significant discovery of peroxidase-like property may extend the applications of conventional EDTA in life science...
December 16, 2016: Analytical Chemistry
Rubén Ahijado-Guzmán, Julia Menten, Janak Prasad, Christina Lambertz, Germán Rivas, Carsten Sönnichsen
We demonstrate the potential of the NanoSPR (nanoscale surface plasmon resonance sensors) method as a simple and cheap tool for the quantitative study of membrane protein-protein interactions. We use NanoSPR to determine the effectiveness of two potential drug candidates that inhibit the protein complex formation between FtsA and ZipA at initial stages of bacterial division. As the NanoSPR method relies on individual gold nanorods as sensing elements, there is no need for fluorescent labels or organic cosolvents, and it provides intrinsically high statistics...
December 22, 2016: ACS Applied Materials & Interfaces
Jiang Ouyang, Zhenjun Liu, Yajing Han, Ke Zeng, Jianping Sheng, Liu Deng, You-Nian Liu
Glycoproteins are important biomarkers and therapeutic targets in clinical diagnostics. The conventional analytical methods for glycoprotein are usually faced with some challenges, such as the complex pretreatment of samples, poor availability, and limited stability of antibody, making them not suitable for point-of-care and on-site application. Herein, we demonstrate a novel miniaturized biofuel cells (BFCs)-based self-powered nanosensor for the specific and sensitive determination of glycoproteins in complex samples through the combination of boronate-affinity molecularly imprinted polymer (MIP) and the boronate affinity functionalized biliroxidase-carbon nanotube nanocomposites...
December 28, 2016: ACS Applied Materials & Interfaces
Verena Wulf, Fabian Knoch, Thomas Speck, Carsten Sönnichsen
Plasmonic gold nanoparticles are normally used as sensor to detect analytes permanently bound to their surface. If the interaction between the analyte and the nanosensor surface is negligible, it only diffuses through the sensor's sensing volume, causing a small temporal shift of the plasmon resonance position. By using a very sensitive and fast detection scheme, we are able to detect these small fluctuations in the plasmon resonance. With the help of a theoretical model consistent with our detection geometry, we determine the analyte's diffusion coefficient...
December 1, 2016: Journal of Physical Chemistry Letters
Javeria Zahra, Shahid Iqbal, Kiran Zahra, Zulha Javed, Muhammad Aslam Shad, Atif Akbar, Muhammad Naeem Ashiq, Furhan Iqbal
Zinc oxide nanoparticles (ZnO NPs) have diverse utility these days ranging from being part of nanosensors to be ingredient of cosmetics. Present study was designed to report the effect of variable doses of ZnO NPs on selected aspects of male albino mice behavior. Nano particles were synthesized by sol-gel auto-combustion method (Data not shown here). 10 week old male albino mice were divided into four experimental groups; group A, B and C were orally supplemented with 50 (low dose), 300 (medium dose) and 600 mg/ml solvent/kg body weight (high dose) of ZnO NPs for 4 days...
December 8, 2016: Neurochemical Research
Katelyn M Spillane, Pavel Tolar
Antibody production and affinity maturation are driven by B cell extraction and internalization of antigen from immune synapses. However, the extraction mechanism remains poorly understood. Here we develop DNA-based nanosensors to interrogate two previously proposed mechanisms, enzymatic liberation and mechanical force. Using antigens presented by either artificial substrates or live cells, we show that B cells primarily use force-dependent extraction and resort to enzymatic liberation only if mechanical forces fail to retrieve antigen...
January 2, 2017: Journal of Cell Biology
Hairulazwan Hashim, Hisataka Maruyama, Taisuke Masuda, Fumihito Arai
Manipulation and injection of single nanosensors with high cell viability is an emerging field in cell analysis. We propose a new method using fluorescence nanosensors with a glass nanoprobe and optical control of the zeta potential. The nanosensor is fabricated by encapsulating a fluorescence polystyrene nanobead into a lipid layer with 1,3,3-trimethylindolino-6'-nitrobenzopyrylospiran (SP), which is a photochromic material. The nanobead contains iron oxide nanoparticles and a temperature-sensitive fluorescent dye, Rhodamine B...
December 1, 2016: Sensors
Alberto Bonanno, Alessandro Sanginario, Simone L Marasso, Beatrice Miccoli, Katarzyna Bejtka, Simone Benetto, Danilo Demarchi
This paper presents a customizable sensing system based on functionalized nanowires (NWs) assembled onto complementary metal oxide semiconductor (CMOS) technology. The Micro-for-Nano (M4N) chip integrates on top of the electronics an array of aluminum microelectrodes covered with gold by means of a customized electroless plating process. The NW assembly process is driven by an array of on-chip dielectrophoresis (DEP) generators, enabling a custom layout of different nanosensors on the same microelectrode array...
November 30, 2016: Sensors
F J Rawson, M T Cole, J M Hicks, J W Aylott, W I Milne, C M Collins, S K Jackson, N J Silman, P M Mendes
With the rapidly increasing demands for ultrasensitive biodetection, the design and applications of new nano-scale materials for development of sensors based on optical and electrochemical transducers have attracted substantial interest. In particular, given the comparable sizes of nanomaterials and biomolecules, there exist plenty of opportunities to develop functional nanoprobes with biomolecules for highly sensitive and selective biosensing, shedding new light on cellular behaviour. Towards this aim, herein we interface cells with patterned nano-arrays of carbon nanofibers forming a nanosensor-cell construct...
December 1, 2016: Scientific Reports
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