keyword
MENU ▼
Read by QxMD icon Read
search

Drosophila optogenetic

keyword
https://www.readbyqxmd.com/read/28332980/a-receptor-and-neuron-that-activate-a-circuit-limiting-sucrose-consumption
#1
Ryan M Joseph, Jennifer S Sun, Edric Tam, John R Carlson
The neural control of sugar consumption is critical for normal metabolism. In contrast to sugar-sensing taste neurons that promote consumption, we identify a taste neuron that limits sucrose consumption in Drosophila. Silencing of the neuron increases sucrose feeding; optogenetic activation decreases it. The feeding inhibition depends on the IR60b receptor, as shown by behavioral analysis and Ca2+ imaging of an IR60b mutant. The IR60b phenotype shows a high degree of chemical specificity when tested with a broad panel of tastants...
March 23, 2017: ELife
https://www.readbyqxmd.com/read/28215335/optogenetic-inhibition-of-apical-constriction-during-drosophila-embryonic-development
#2
G Guglielmi, S De Renzis
Morphogenesis of multicellular organisms is driven by changes in cell behavior, which happen at precise locations and defined developmental stages. Therefore, the studying of morphogenetic events would greatly benefit from tools that allow the perturbation of cell activity with spatial and temporal precision. We recently developed an optogenetic approach to modulate cell contractility with cellular precision and on fast (seconds) timescales during Drosophila embryogenesis. We present here a protocol to handle genetically engineered photosensitive Drosophila embryos and achieve light-mediated inhibition of apical constriction during tissue invagination...
2017: Methods in Cell Biology
https://www.readbyqxmd.com/read/28118601/the-spatiotemporal-limits-of-developmental-erk-signaling
#3
Heath E Johnson, Yogesh Goyal, Nicole L Pannucci, Trudi Schüpbach, Stanislav Y Shvartsman, Jared E Toettcher
Animal development is characterized by signaling events that occur at precise locations and times within the embryo, but determining when and where such precision is needed for proper embryogenesis has been a long-standing challenge. Here we address this question for extracellular signal regulated kinase (Erk) signaling, a key developmental patterning cue. We describe an optogenetic system for activating Erk with high spatiotemporal precision in vivo. Implementing this system in Drosophila, we find that embryogenesis is remarkably robust to ectopic Erk signaling, except from 1 to 4 hr post-fertilization, when perturbing the spatial extent of Erk pathway activation leads to dramatic disruptions of patterning and morphogenesis...
January 23, 2017: Developmental Cell
https://www.readbyqxmd.com/read/28115483/gap-junction-mediated-signaling-from-motor-neurons-regulates-motor-generation-in-the-central-circuits-of-larval-drosophila
#4
Teruyuki Matsunaga, Hiroshi Kohsaka, Akinao Nose
In this study, we used the peristaltic crawling of Drosophila larvae as a model to study how motor patterns are regulated by central circuits. We built an experimental system that allows simultaneous application of optogenetics and calcium imaging to the isolated ventral nerve cord (VNC). We then investigated the effects of manipulating local activity of motor neurons (MNs) on fictive locomotion observed as waves of MN activity propagating along neuromeres. Optical inhibition of MNs with halorhodopsin3 (NpHR3) in a middle segment (A4, A5 or A6), but not other segments, dramatically decreased the frequency of the motor waves...
January 23, 2017: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/28114289/optogenetic-inhibition-of-behavior-with-anion-channelrhodopsins
#5
Farhan Mohammad, James C Stewart, Stanislav Ott, Katarina Chlebikova, Jia Yi Chua, Tong-Wey Koh, Joses Ho, Adam Claridge-Chang
Optogenetics uses light exposure to manipulate physiology in genetically modified organisms. Abundant tools for optogenetic excitation are available, but the limitations of current optogenetic inhibitors present an obstacle to demonstrating the necessity of neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit neural systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, thus demonstrating their broad utility in the circuit analysis of behavior...
March 2017: Nature Methods
https://www.readbyqxmd.com/read/28113210/fim2c-a-multi-colour-multi-purpose-imaging-system-to-manipulate-and-analyse-animal-behaviour
#6
Benjamin Risse, Nils Otto, Dimitri Berh, Xiaoyi Jiang, Matthias Kiel, Christian Klambt
In vivo whole-body imaging of small animals plays an important role for biomedical studies. In particular, animals like the fruit fly Drosophila melanogaster or the nematode Caenorhabditis elegans are popular model organisms for preclinical research since they offer sophisticated genetic tool-kits. Recording these translucent animals with high contrast in a large arena is however not trivial. Furthermore fluorescent proteins are widely used to mark cells in vivo and report their functions. This paper introduces a novel optical imaging technique called FIM2c enabling simultaneous detection of the animals posture and movement as well as fluorescent markers like GFP...
May 18, 2016: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28065655/precision-optogenetic-tool-for-selective-single-and-multiple-cell-ablation-in-a-live-animal-model-system
#7
Kalpana Makhijani, Tsz-Leung To, Rubén Ruiz-González, Céline Lafaye, Antoine Royant, Xiaokun Shu
Cell ablation is a strategy to study cell lineage and function during development. Optogenetic methods are an important cell-ablation approach, and we have previously developed a mini singlet oxygen generator (miniSOG) tool that works in the living Caenorhabditis elegans. Here, we use directed evolution to generate miniSOG2, an improved tool for cell ablation via photogenerated reactive oxygen species. We apply miniSOG2 to a far more complex model animal system, Drosophila melanogaster, and demonstrate that it can be used to kill a single neuron in a Drosophila larva...
January 19, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28029094/visual-projection-neurons-in-the-drosophila-lobula-link-feature-detection-to-distinct-behavioral-programs
#8
Ming Wu, Aljoscha Nern, W Ryan Williamson, Mai M Morimoto, Michael B Reiser, Gwyneth M Card, Gerald M Rubin
Visual projection neurons (VPNs) provide an anatomical connection between early visual processing and higher brain regions. Here we characterize lobula columnar (LC) cells, a class of Drosophila VPNs that project to distinct central brain structures called optic glomeruli. We anatomically describe 22 different LC types and show that, for several types, optogenetic activation in freely moving flies evokes specific behaviors. The activation phenotypes of two LC types closely resemble natural avoidance behaviors triggered by a visual loom...
December 28, 2016: ELife
https://www.readbyqxmd.com/read/27818173/the-trp-channels-pkd2-nompc-and-trpm-act-in-cold-sensing-neurons-to-mediate-unique-aversive-behaviors-to-noxious-cold-in-drosophila
#9
Heather N Turner, Kevin Armengol, Atit A Patel, Nathaniel J Himmel, Luis Sullivan, Srividya Chandramouli Iyer, Surajit Bhattacharya, Eswar Prasad R Iyer, Christian Landry, Michael J Galko, Daniel N Cox
The basic mechanisms underlying noxious cold perception are not well understood. We developed Drosophila assays for noxious cold responses. Larvae respond to near-freezing temperatures via a mutually exclusive set of singular behaviors-in particular, a full-body contraction (CT). Class III (CIII) multidendritic sensory neurons are specifically activated by cold and optogenetic activation of these neurons elicits CT. Blocking synaptic transmission in CIII neurons inhibits CT. Genetically, the transient receptor potential (TRP) channels Trpm, NompC, and Polycystic kidney disease 2 (Pkd2) are expressed in CIII neurons, where each is required for CT...
December 5, 2016: Current Biology: CB
https://www.readbyqxmd.com/read/27730580/performing-chromophore-assisted-laser-inactivation-in-drosophila-embryos-using-gfp
#10
Anne Pélissier-Monier, Bénédicte Sanson, Bruno Monier
Chromophore-assisted laser inactivation (CALI) is an optogenetic technique in which light-induced release of reactive oxygen species triggers acute inactivation of a protein of interest, with high spatial and temporal resolution. At its simplest, selective protein inactivation can be achieved via the genetic fusion of the protein to a photosensitizer such as EGFP, and using standard optical setups such as laser scanning confocal microscopes. Although use of CALI in Drosophila is relatively recent, this technique can be a powerful complement to developmental genetics, especially in vivo as it allows visualization of the immediate consequences of local protein inactivation when coupled to time-lapse microscopy analysis...
2016: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27617442/odorant-responses-and-courtship-behaviors-influenced-by-at4-neurons-in-drosophila
#11
Svetlana Pitts, Elizabeth Pelser, Julian Meeks, Dean Smith
In insects, pheromones function as triggers to elicit complex behavior programs, such as courtship and mating behavior. In most species, the neurons tuned to pheromones are localized in a specific subset of olfactory sensilla located on the antenna called trichoid sensilla. In Drosophila there are two classes of trichoid sensilla, at1 sensilla that contain the dendrites of a single neuron that is specifically tuned to the male-specific pheromone 11-cis vaccenyl acetate (cVA), and at4 sensilla that contain three neurons with relatively poorly defined chemical specificity and function...
2016: PloS One
https://www.readbyqxmd.com/read/27585032/olfactory-behaviors-assayed-by-computer-tracking-of-drosophila-in-a-four-quadrant-olfactometer
#12
Chun-Chieh Lin, Olena Riabinina, Christopher J Potter
A key challenge in neurobiology is to understand how neural circuits function to guide appropriate animal behaviors. Drosophila melanogaster is an excellent model system for such investigations due to its complex behaviors, powerful genetic techniques, and compact nervous system. Laboratory behavioral assays have long been used with Drosophila to simulate properties of the natural environment and study the neural mechanisms underlying the corresponding behaviors (e.g. phototaxis, chemotaxis, sensory learning and memory)(1-3)...
August 20, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27572257/functional-integration-of-a-serotonergic-neuron-in-the-drosophila-antennal-lobe
#13
Xiaonan Zhang, Quentin Gaudry
Serotonin plays a critical role in regulating many behaviors that rely on olfaction and recently there has been great effort in determining how this molecule functions in vivo. However, it remains unknown how serotonergic neurons that innervate the first olfactory relay respond to odor stimulation and how they integrate synaptically into local circuits. We examined the sole pair of serotonergic neurons that innervates the Drosophila antennal lobe (the first olfactory relay) to characterize their physiology, connectivity, and contribution to pheromone processing...
August 30, 2016: ELife
https://www.readbyqxmd.com/read/27484401/high-brightness-organic-light-emitting-diodes-for-optogenetic-control-of-drosophila-locomotor-behaviour
#14
Andrew Morton, Caroline Murawski, Stefan R Pulver, Malte C Gather
Organic light emitting diodes (OLEDs) are in widespread use in today's mobile phones and are likely to drive the next generation of large area displays and solid-state lighting. Here we show steps towards their utility as a platform technology for biophotonics, by demonstrating devices capable of optically controlling behaviour in live animals. Using devices with a pin OLED architecture, sufficient illumination intensity (0.3 mW.mm(-2)) to activate channelrhodopsins (ChRs) in vivo was reliably achieved at low operating voltages (5 V)...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27479324/circadian-neuron-feedback-controls-the-drosophila-sleep-activity-profile
#15
Fang Guo, Junwei Yu, Hyung Jae Jung, Katharine C Abruzzi, Weifei Luo, Leslie C Griffith, Michael Rosbash
Little is known about the ability of Drosophila circadian neurons to promote sleep. Here we show, using optogenetic manipulation and video recording, that a subset of dorsal clock neurons (DN1s) are potent sleep-promoting cells that release glutamate to directly inhibit key pacemaker neurons. The pacemakers promote morning arousal by activating these DN1s, implying that a late-day feedback circuit drives midday siesta and night-time sleep. To investigate more plastic aspects of the sleep program, we used a calcium assay to monitor and compare the real-time activity of DN1 neurons in freely behaving males and females...
August 18, 2016: Nature
https://www.readbyqxmd.com/read/27445732/artificial-induction-of-associative-olfactory-memory-by-optogenetic-and-thermogenetic-activation-of-olfactory-sensory-neurons-and-octopaminergic-neurons-in-drosophila-larvae
#16
Takato Honda, Chi-Yu Lee, Ken Honjo, Katsuo Furukubo-Tokunaga
The larval brain of Drosophila melanogaster provides an excellent system for the study of the neurocircuitry mechanism of memory. Recent development of neurogenetic techniques in fruit flies enables manipulations of neuronal activities in freely behaving animals. This protocol describes detailed steps for artificial induction of olfactory associative memory in Drosophila larvae. In this protocol, the natural reward signal is substituted by thermogenetic activation of octopaminergic neurons in the brain. In parallel, the odor signal is substituted by optogenetic activation of a specific class of olfactory receptor neurons...
2016: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/27441388/dopaminergic-neurons-write-and-update-memories-with-cell-type-specific-rules
#17
Yoshinori Aso, Gerald M Rubin
Associative learning is thought to involve parallel and distributed mechanisms of memory formation and storage. In Drosophila, the mushroom body (MB) is the major site of associative odor memory formation. Previously we described the anatomy of the adult MB and defined 20 types of dopaminergic neurons (DANs) that each innervate distinct MB compartments (Aso et al., 2014a, 2014b). Here we compare the properties of memories formed by optogenetic activation of individual DAN cell types. We found extensive differences in training requirements for memory formation, decay dynamics, storage capacity and flexibility to learn new associations...
2016: ELife
https://www.readbyqxmd.com/read/27373835/behavior-reveals-selective-summation-and-max-pooling-among-olfactory-processing-channels
#18
Joseph S Bell, Rachel I Wilson
The olfactory system is divided into processing channels (glomeruli), each receiving input from a different type of olfactory receptor neuron (ORN). Here we investigated how glomeruli combine to control behavior in freely walking Drosophila. We found that optogenetically activating single ORN types typically produced attraction, although some ORN types produced repulsion. Attraction consisted largely of a behavioral program with the following rules: at fictive odor onset, flies walked upwind, and at fictive odor offset, they reversed...
July 20, 2016: Neuron
https://www.readbyqxmd.com/read/27281494/cyclic-nucleotide-signaling-is-required-during-synaptic-refinement-at-the-drosophila-neuromuscular-junction
#19
Fernando Vonhoff, Haig Keshishian
The removal of miswired synapses is a fundamental prerequisite for normal circuit development, leading to clinical problems when aberrant. However, the underlying activity-dependent molecular mechanisms involved in synaptic pruning remain incompletely resolved. Here the dynamic properties of intracellular calcium oscillations and a role for cAMP signaling during synaptic refinement in intact Drosophila embryos were examined using optogenetic tools. We provide In vivo evidence at the single gene level that the calcium-dependent adenylyl cyclase rutabaga, the phosphodiesterase dunce, the kinase PKA, and Protein Phosphatase 1 (PP1) all operate within a functional signaling pathway to modulate Sema2a-dependent chemorepulsion...
January 2017: Developmental Neurobiology
https://www.readbyqxmd.com/read/27269519/the-timed-depolarization-of-morning-and-evening-oscillators-phase-shifts-the-circadian-clock-of-drosophila
#20
Saskia Eck, Charlotte Helfrich-Förster, Dirk Rieger
Phase response curves (PRCs) for light or temperature stimuli have been shown to be most valuable in understanding how circadian clocks are entrained to daily environmental cycles. Nowadays, PRC experiments in which clock neurons are manipulated in a temporally restricted manner by thermogenetic or optogenetic tools are also useful to comprehend clock network properties. Here, we temporally depolarized specific clock neurons of Drosophila melanogaster by activating temperature-sensitive dTrpA1 channels to unravel their role in phase shifting the flies' activity rhythm...
October 2016: Journal of Biological Rhythms
keyword
keyword
68404
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"