keyword
MENU ▼
Read by QxMD icon Read
search

TEMS

keyword
https://www.readbyqxmd.com/read/28930685/human-tissue-resident-memory-t-cells-are-defined-by-core-transcriptional-and-functional-signatures-in-lymphoid-and-mucosal-sites
#1
Brahma V Kumar, Wenji Ma, Michelle Miron, Tomer Granot, Rebecca S Guyer, Dustin J Carpenter, Takashi Senda, Xiaoyun Sun, Siu-Hong Ho, Harvey Lerner, Amy L Friedman, Yufeng Shen, Donna L Farber
Tissue-resident memory T cells (TRMs) in mice mediate optimal protective immunity to infection and vaccination, while in humans, the existence and properties of TRMs remain unclear. Here, we use a unique human tissue resource to determine whether human tissue memory T cells constitute a distinct subset in diverse mucosal and lymphoid tissues. We identify a core transcriptional profile within the CD69(+) subset of memory CD4(+) and CD8(+) T cells in lung and spleen that is distinct from that of CD69(-) TEM cells in tissues and circulation and defines human TRMs based on homology to the transcriptional profile of mouse CD8(+) TRMs...
September 19, 2017: Cell Reports
https://www.readbyqxmd.com/read/28930577/exosome-enrichment-by-ultracentrifugation-and-size-exclusion-chromatography
#2
Yong Qin Koh, Fatema B Almughlliq, Kanchan Vaswani, Hassendrini N Peiris, Murray D Mitchell
Exosomes are a subset of extracellular vesicles (EVs) that have important roles in intercellular communication. They contain and carry bioactive molecules within their membranes which are delivered to target cells. Reproducible isolation and enrichment of these exosomes will aid in evaluation of cellular communication. We present an approach that involved the pre-processing of plasma, combined with ultracentrifugation (UC) and size exclusion chromatography (SEC) to isolate EVs and subsequently enrich exosomes...
January 1, 2018: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/28930435/new-triplet-sensitization-routes-for-photon-upconversion-thermally-activated-delayed-fluorescence-molecules-inorganic-nanocrystals-and-singlet-to-triplet-absorption
#3
Nobuhiro Yanai, Nobuo Kimizuka
Photon upconversion based on triplet-triplet annihilation (TTA-UC) has attracted much interest because of its possible applications to renewable energy production and biological fields. In particular, the UC of near-infrared (NIR) light to visible (vis) light is imperative to overcome the Shockley-Queisser limit of single-junction photovoltaic cells, and the efficiency of photocatalytic hydrogen production from water can also be improved with the aid of vis-to-ultraviolet (UV) UC. However, both processes have met limitations in the wavelength range, efficiency, and sensitivity for weak incident light...
September 20, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28930353/interplay-between-bulk-self-assembly-interfacial-and-foaming-properties-in-a-catanionic-surfactant-mixture-of-varying-composition
#4
José Ferreira, Alesya Mikhailovskaya, Alexis Chenneviere, Frédéric Restagno, Fabrice Cousin, François Muller, Jéril Degrouard, Anniina Salonen, Eduardo F Marques
The self-aggregation, surface properties and foamability of the catanionic surfactant mixture cetyltrimethylammonium bromide (CTAB)/sodium octyl sulfonate (SOSo) have been investigated to obtain insight on the relation between bulk nanostructures, surfactant packing, and foam stability and aging. Light microscopy, SANS, cryo-TEM, DLS, surface tension, rheometry and direct photography were used to characterize mixtures with varying CTAB molar fraction, xCTAB. In the bulk, self-assembly is richer in the excess CTAB region than in the excess SOSo one...
September 20, 2017: Soft Matter
https://www.readbyqxmd.com/read/28929150/developing-an-efficient-nico2s4-cocatalyst-for-improving-the-visible-light-h2-evolution-performance-of-cds-nanoparticles
#5
Junwen Peng, Junli Xu, Zhaoyu Wang, Zhengxin Ding, Sibo Wang
Developing efficient alternatives to the widely used Pt cocatalyst in photocatalytic H2O splitting is of great importance in view of large-scale production of clear H2 energy. Herein, we report the facile synthesis of NiCo2S4 and its first use as a highly active and cost-affordable cocatalyst to boost visible light H2 generation with the CdS semiconductor. The synthesized NiCo2S4/CdS composite materials are fully characterized by various techniques including X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy-dispersive X-ray (EDX) spectroscopy, X-ray photoelectron spectroscopy (XPS), UV-Vis diffusion reflectance spectroscopy (DRS), and N2 adsorption measurements...
September 20, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28928850/spiral-computed-tomography-evaluation-of-rabbit-vx2-hepatic-tumors-treated-with-20-khz-ultrasound-and-microbubbles
#6
Zhi-Yong Shen, Chun Liu, Ming-Feng Wu, Hai-Feng Shi, Yu-Feng Zhou, Wei Zhuang, Gan-Lin Xia
The aim of the present study was to explore the therapeutic effect of 20 kHz ultrasound (US) and microbubbles (MBs) on rabbit VX2 liver tumors by spiral computed tomography (CT) scanning. A total of 16 New Zealand rabbits with hepatic VX2 tumors were divided into four groups: Control, MB, low-frequency US and US + MB. The treatment effect was evaluated by spiral CT scanning prior to, during and following treatment (at 0 weeks and the end of 1 and 2 weeks). The tumor growth rate was recorded. The specimens of VX2 tumors were collected for histological examination and transmission electron microscopy (TEM)...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28928385/dually-functioned-core-shell-nayf4-er-3-yb-3-nayf4-tm-3-yb-3-nanoparticles-as-nano-calorifiers-and-nano-thermometers-for-advanced-photothermal-therapy
#7
Yanqiu Zhang, Baojiu Chen, Sai Xu, Xiangping Li, Jinsu Zhang, Jiashi Sun, Hui Zheng, Lili Tong, Guozhu Sui, Hua Zhong, Haiping Xia, Ruinian Hua
To realize photothermal therapy (PTT) of cancer/tumor both the photothermal conversion and temperature detection are required. Usually, the temperature detection in PTT needs complicated instruments, and the therapy process is out of temperature control in the present investigations. In this work, we attempt to develop a novel material for achieving both the photothermal conversion and temperature sensing and control at the same time. To this end, a core-shell structure with NaYF4:Er(3+)/Yb(3+) core for temperature detection and NaYF4:Tm(3+)/Yb(3+) shell for photothermal conversion was designed and prepared...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28928140/selenoprotein-t-is-a-novel-ost-subunit-that-regulates-upr-signaling-and-hormone-secretion
#8
Abdallah Hamieh, Dorthe Cartier, Houssni Abid, André Calas, Carole Burel, Christine Bucharles, Cedric Jehan, Luca Grumolato, Marc Landry, Patrice Lerouge, Youssef Anouar, Isabelle Lihrmann
Selenoprotein T (SelT) is a recently characterized thioredoxin-like protein whose expression is very high during development, but is confined to endocrine tissues in adulthood where its function is unknown. We report here that SelT is required for adaptation to the stressful conditions of high hormone level production in endocrine cells. Using immunofluorescence and TEM immunogold approaches, we find that SelT is expressed at the endoplasmic reticulum membrane in all hormone-producing pituitary cell types. SelT knockdown in corticotrope cells promotes unfolded protein response (UPR) and ER stress and lowers endoplasmic reticulum-associated protein degradation (ERAD) and hormone production...
September 19, 2017: EMBO Reports
https://www.readbyqxmd.com/read/28927630/sustainable-thermoplastic-elastomers-derived-from-cellulose-fatty-acid-and-furfural-via-atrp-and-click-chemistry
#9
Juan Yu, Chuanwei Lu, Chunpeng Wang, Jifu Wang, Yimin Fan, Fuxiang Chu
Cellulose-based thermoplastic elastomers (TPEs) have attracted considerable attention because of their rigid backbone, good mechanical properties, renewable nature and abundance. In the present study, sustainable TPEs based on ethyl cellulose (EC), fatty acid and furfural were generated by the combination of ATRP and "click chemistry". To fabricate sustainable TPEs with higher toughness, a range of polymers, including mono random-copolymer poly(tetrahydrofurfuryl methacrylate-co-lauryl methacrylate) (P(THFMA-co-LMA), dual polymer side chains PTHFMA and PLMA, and mono-block copolymer PTHFMA-b-PLMA, were designed as side chains to fabricate EC brush copolymers with random, dual or block side chain architectures using the "grafting from" and "grafting onto" methods...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28927615/green-thermal-assisted-synthesis-and-characterization-of-novel-cellulose-mg-oh-2-nanocomposite-in-peg-naoh-solvent
#10
Nikolai Ponomarev, Eveliina Repo, Varsha Srivastava, Mika Sillanpää
Synthesis of nanocomposites was performed using microcrystalline cellulose (MCC), MgCl2 in PEG/NaOH solvent by a thermal-assisted method at different temperatures by varying time and the amount of MCC. Results of XRD, FTIR, and EDS mapping showed that the materials consisted of only cellulose (CL) and magnesium hydroxide (MH). According to FTIR and XRD, it was found that crystallinity of MH in cellulose nanocomposites is increased with temperature and heating time and decreased with increasing of cellulose amount...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28927601/new-technique-in-starch-nanoparticles-synthesis
#11
Manal A El-Sheikh
Starch nanoparticles (StNPs) were previously prepared using severe mechanical, physical and/or chemical conditions that takes too long time to produce a little yield of uncontrolled large size of StNP. The current work presents a new technique for the synthesis of StNPs based on the combination between sodium hydroxide and glycerol in aqueous medium during the synthesis process while the precipitation of the StNPs is performed under homogenization at ambient conditions. The new technique is based on three assumptions: 1) the dual impact of sodium hydroxide and glycerol on the swelling and gelatinization of starch as a pre-stage of the synthesis of (StNPs1), 2) the dual impact of glycerol and cooking of starch on the swelling and gelatinization of starch as a pre-stage of the formation of (StNPs2), and 3) the precipitation under homogenization at ambient conditions and its effect on breaking the H- bonds of the starch molecule in both StNPs1 and StNPs2...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28927589/molecular-dynamics-of-carrageenan-composites-reinforced-with-cloisite-na-montmorillonite-nanoclay
#12
M J Sanchis, M Carsí, M Culebras, C M Gómez, S Rodriguez, F G Torres
Nanocomposites comprising biodegradable carrageenan and glycerol (KCg) as the host polymer, with different contents of natural montmorillonite (MMT) as filler, were prepared by a solution casting process. Different techniques have been used to determine the interaction/behavior among the different components of the samples such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Transmission electron microscope (TEM) and, mainly, Dielectric relaxation spectroscopy (DRS). FTIR indicates hydrogen interaction between carrageenan matrix and silicate that is confirmed by the XRD data indicating some kind of carrageenan intercalation between the MMT layers...
November 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28927274/fluoride-induced-autophagy-via-the-regulation-of-mtor-phosphorylation-in-mice-leydig-cells
#13
Jianhai Zhang, Yuchen Zhu, Yan Shi, Yongli Han, Chen Liang, Zhiyuan Feng, Heping Zheng, Michelle Eng, Jundong Wang
Abstracts:Fluoride is known to impair testicular functions and decrease testosterone levels, yet the underlying mechanisms remain inconclusive. The objective of this study is to investigate the roles of autophagy in fluoride-induced male reproductive toxicity by both in vivo and in vitro Leydig cell models. Using Transmission Electron Microscopy (TEM) and Monodansylcadaverine (MDC) staining, we observed increasing numbers of autophagosomes in testicular tissue, especially in Leydig cells of fluoride-exposed mice...
September 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28927033/all-trans-retinoic-acid-enhances-temozolomide-induced-autophagy-in-human-glioma-cells-u251-via-targeting-keap1-nrf2-are-signaling-pathway
#14
Lin Shi, Hongyuan Li, Yang Zhan
The present study evaluated the retinoic acid (RA) enhancement of temozolomide (TMZ) effects on the human glioma cells U251 and explored its underlying molecular mechanism. The cell growth was detected using the MTT assay and the cell cycle was assessed by flow cytometry. Cell apoptosis was analyzed by Annexin V/propidium iodide staining, and the cell morphology was evaluated using transmission electron microscopy (TEM). Additionally, reverse transcription-PCR and western blot analysis were applied to detect the mRNA and protein levels...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927021/acetylene-gas-sensing-properties-of-layer-by-layer-self-assembled-ag-decorated-tin-dioxide-graphene-nanocomposite-film
#15
Chuanxing Jiang, Dongzhi Zhang, Nailiang Yin, Yao Yao, Talgar Shaymurat, Xiaoyan Zhou
This paper demonstrates an acetylene gas sensor based on an Ag-decorated tin dioxide/reduced graphene oxide (Ag-SnO₂/rGO) nanocomposite film, prepared by layer-by-layer (LbL) self-assembly technology. The as-prepared Ag-SnO₂/rGO nanocomposite was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and Raman spectrum. The acetylene sensing properties were investigated using different working temperatures and gas concentrations. An optimal temperature of 90 °C was determined, and the Ag-SnO₂/rGO nanocomposite sensor exhibited excellent sensing behaviors towards acetylene, in terms of response, repeatability, stability and response/recovery characteristics, which were superior to the pure SnO₂ and SnO₂/rGO film sensors...
September 18, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28926823/preparation-characterization-and-application-of-urease-nanoparticles-for-construction-of-an-improved-potentiometric-urea-biosensor
#16
Seema Jakhar, C S Pundir
The nanoparticles (NPs) aggregates of commercial urease from jack beans (Canavalia ensiformis) were prepared by desolvation and glutaraldehyde crosslinking and functionalized by cysteamine dihydrochloride. These enzyme nanoparticles (ENPs) were characterized by transmission electron microscopy (TEM), UV and Fourier transform infrared (FTIR) spectroscopy. The TEM images of urease NPs showed their size in the range, 18-100nm with an average of 51.2nm. The ENPs were more active and stable with a longer shelf life than native enzyme molecules...
September 6, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28926806/simultaneous-removal-of-sulfides-and-benzene-in-fcc-gasoline-by-in-situ-hydrogenation-over-nilain-zro2-r-al2o3
#17
Lei Wang, Qian Liu, Chunyu Jing, Jiajia Yin, Nchare Mominou, Shuzhen Li
The ZrO2-r-Al2O3 composite was developed and co-modified with nickel, lanthanum and indium species by the impregnation method. A fixed-bed reactor was utilized to investigate the activity of the resulting catalysts in the removal of sulfides and benzene in fluid catalytic cracking gasoline. The resultant samples were characterized using XRD, TEM, XPS, TGA, H2-TPR, NH3-TPD and N2 adsorption/desorption techniques. XRD analysis indicated no agglomeration of Ni and La species, in good agreement with the TEM image...
September 6, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28926707/medieval-gilding-technology-of-historical-metal-threads-revealed-by-electron-optical-and-micro-raman-spectroscopic-study-of-focused-ion-beam-milled-cross-sections
#18
Tamás Gábor Weiszburg, Katalin Gherdán, Kitti Ratter, Norbert Zajzon, Zsolt Bendő, György Z Radnoczi, Ágnes Takács, Tamás Váczi, Gábor Varga, György Szakmány
Although gilt silver threads were widely used for decorating historical textiles, their manufacturing techniques have been elusive for centuries. Contemporary written sources give only limited, sometimes ambiguous information, and detailed cross-sectional study of the microscale soft noble metal objects has been hindered by sample preparation. In this work, to give a thorough characterization of historical gilt silver threads, nano- and microscale textural, chemical and structural data on cross sections, prepared by focused ion beam milling, were collected, using various electron-optical methods (high-resolution scanning electron microscopy (SEM), wavelength-dispersive electron probe microanalysis (EPMA), electron back-scattered diffraction (EBSD) combined with energy-dispersive electron probe microanalysis (EDX), transmission electron microscopy (TEM) combined with EDX, and micro-Raman spectroscopy...
September 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28926094/curcumin-regulates-proliferation-autophagy-and-apoptosis-in-gastric-cancer-cells-by-affecting-pi3k-and-p53-signaling
#19
Hongbing Fu, Changming Wang, Dejun Yang, Xin Zhang, Ziran Wei, Zhenxin Zhu, Jiapeng Xu, Zunqi Hu, Yu Zhang, Weimin Wang, Ronglin Yan, Qingping Cai
In this study, we aimed to investigate the effects of curcumin on cell activities of gastric cancer (GC), and the connection between curcumin and P53 as well as PI3K signaling. This study was conducted with two cell lines SGC-7901 and BGC-823, both were exposed to curcumin at the concentrations of 0 µM, 10 µM, 20 µM and 40 µM. MTT assay, flow cytometry (FCM) assay, transmission electron microscopy (TEM) were used to study the underlying mechanisms of curcumin in respective of proliferation, apoptosis and autophagy...
September 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28925796/should-transmission-electron-microscopy-and-ultrastructural-cilia-evaluation-remain-part-of-the-diagnostic-work-up-for-primary-ciliary-dyskinesia
#20
Claudius Werner, Panayiotis Kouis
The diagnostic work-up for primary ciliary dyskinesia (PCD) traditionally includes ciliary ultrastructural assessment using transmission electron microscopy (TEM). However, the identification of genetic variants of PCD that are missed by TEM, along with the development of novel diagnostic modalities for PCD that allow structural evaluation of cilia, such as immunofluorescence analysis and the increased availability of genetic testing, calls into questioning the contemporary role of TEM in the diagnostic work-up for PCD...
September 19, 2017: Ultrastructural Pathology
keyword
keyword
68395
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"