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https://www.readbyqxmd.com/read/29345852/cadmium-and-cadmium-tolerant-soil-bacteria-in-cacao-crops-from-northeastern-colombia
#1
Daniel Bravo, Sergio Pardo-Díaz, Javier Benavides-Erazo, Gersain Rengifo-Estrada, Olivier Braissant, Clara Leon-Moreno
AIMS: This research aims to assess total-cadmium soil content and microbiological aspects to understand the dynamics of culturable cadmium-tolerant bacteria (CdtB) in cacao soils from northeastern Colombia. METHODS AND RESULTS: An integration of inverted dish plating, Cd determination and a microcalorimetry assay (IMC) was carried out. A farm in Boyacá showed the highest level of total soil Cd (3.74 mg kg-1 ) followed by farms in Santander and Arauca (2.76 and 1...
January 18, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29345200/distinct-niches-within-the-extracellular-matrix-dictate-fibroblast-function-in-cell-free-3d-lung-tissue-cultures
#2
Gerald Burgstaller, Arunima Sengupta, Sarah Vierkotten, Gerhard Preissler, Michael Lindner, Jürgen Behr, Melanie Königshoff, Oliver Eickelberg
Instructive cues from the extracellular matrix (ECM) and their functional interplay with cells play pivotal roles for development, tissue repair, and disease. However, the precise nature of this interplay remains elusive. We used an innovative 3D cell culture ECM model by decellularizing ex vivo lung tissue scaffolds (d3D-LTCs) derived from diseased and healthy mouse lungs, which widely mimics the native (patho)physiological in vivo microenvironment. We successfully repopulated all d3D-LTCs with primary human and murine fibroblasts...
January 18, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29345093/rapid-fabrication-of-detachable-three-dimensional-tissues-by-layering-of-cell-sheets-with-heating-centrifuge
#3
Yuji Haraguchi, Yuki Kagawa, Akiyuki Hasegawa, Hirotsugu Kubo, Tatsuya Shimizu
Confluent cultured cells on a temperature-responsive culture dish can be harvested as an intact cell sheet by decreasing temperature below 32°C. A three-dimensional (3-D) tissue can be fabricated by the layering of cell sheets. A resulting 3-D multi-layered cell sheet-tissue on a temperature-responsive culture dish can be also harvested without any damage by only temperature decreasing. For shortening the fabrication time of the 3-D multi-layered constructs, we attempted to layer cell sheets on a temperature-responsive culture dish with centrifugation...
January 18, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29337880/the-sodium-and-potassium-content-of-the-most-commonly-available-street-foods-in-tajikistan-and-kyrgyzstan-in-the-context-of-the-feedcities-project
#4
Inês Lança de Morais, Nuno Lunet, Gabriela Albuquerque, Marcello Gelormini, Susana Casal, Albertino Damasceno, Olívia Pinho, Pedro Moreira, Jo Jewell, João Breda, Patrícia Padrão
This cross-sectional study is aimed at assessing sodium (Na) and potassium (K) content and the molar Na:K ratios of the most commonly available ready-to-eat street foods in Tajikistan and Kyrgyzstan. Four different samples of each of these foods were collected and 62 food categories were evaluated through bromatological analysis. Flame photometry was used to quantify sodium and potassium concentrations. The results show that home-made foods can be important sources of sodium. In particular, main dishes and sandwiches, respectively, contain more than 1400 and nearly 1000 mg Na in an average serving and provide approximately 70% and 50% of the maximum daily recommended values...
January 16, 2018: Nutrients
https://www.readbyqxmd.com/read/29336219/asymmetry-between-sister-cells-of-pluripotent-stem-cells-at-the-onset-of-differentiation
#5
Shogo Nakamura, Atsushi Maruyama, Yuki Kondo, Ayumu Kano, Olga Maria De Sousa, Masahiro Iwahashi, Bayar Hexig, Toshihiro Akaike, Jingyue Li, Yohei Hayashi, Kiyoshi Ohnuma
Various somatic stem cells divide asymmetrically; however, it is not known whether embryonic stem cells (ESCs) divide symmetrically or asymmetrically, not only while maintaining an undifferentiated state, but also at the onset of differentiation. Here, we observed single ESCs using time-lapse imaging, and compared sister cell pairs derived from the same mother cell in either the maintenance or differentiation medium. Mouse ESCs were cultured on E-cadherin-coated glass-based dishes, which allowed us to trace single cells...
January 16, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29334242/development-and-disease-in-a-dish-the-epigenetics-of-neurodevelopmental-disorders
#6
Emily Ma Lewis, Kristen L Kroll
Human neurodevelopmental disorders (NDDs) involve mutations in hundreds of individual genes, with over-representation in genes encoding proteins that alter chromatin structure to modulate gene expression. Here, we highlight efforts to model these NDDs through in vitro differentiation of patient-specific induced pluripotent stem cells into neurons. We discuss how epigenetic regulation controls normal cortical development, how mutations in several classes of epigenetic regulators contribute to NDDs, and approaches for modeling cortical development and function using both directed differentiation and formation of cerebral organoids...
January 15, 2018: Epigenomics
https://www.readbyqxmd.com/read/29332501/-plates-and-dishes-smash-married-couples-clash-cultural-and-social-barriers-to-help-seeking-among-women-domestic-violence-survivors-in-kyrgyzstan
#7
Saltanat Childress
This article develops a grounded theory of help-seeking to investigate the social and cultural determinants of help-seeking among Kyrgyz women who have experienced domestic violence. Results indicate that cultural traditions and social norms-most notably the social construction of marriage, the shame associated with divorce, and the status of daughters-in-law in Kyrgyz society-are used to justify domestic violence and prevent victims from seeking help. The proposed theory and results suggest that scholars, policymakers, and front-line contacts must emphasize dispelling myths, misconceptions, and traditional beliefs about gender and marriage to break the abusive dynamics and provide professional help...
August 1, 2017: Violence Against Women
https://www.readbyqxmd.com/read/29327302/diffuse-idiopathic-skeletal-hyperostosis-is-associated-with-lumbar-spinal-stenosis-requiring-surgery
#8
Kentaro Yamada, Shigenobu Satoh, Hiroshi Hashizume, Noriko Yoshimura, Ryohei Kagotani, Yuyu Ishimoto, Yuichiro Abe, Hiromitsu Toyoda, Hidetomi Terai, Takeshi Masuda, Shigeyuki Muraki, Hiroaki Nakamura, Munehito Yoshida
Factors related to the onset and progression of lumbar spinal stenosis (LSS) have not yet been identified. Diffuse idiopathic skeletal hyperostosis (DISH) increases mechanical loading on the non-fused lumbar levels and may therefore lead to LSS. This cross-sectional study aimed to identify associations between LSS and DISH. This study included 2363 consecutive patients undergoing surgery for LSS and 787 general inhabitants without symptoms of LSS as participants of the population-based cohort study, Research on Osteoarthritis/Osteoporosis Against Disability...
January 11, 2018: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29321686/effect-of-monitoring-salt-concentration-of-home-prepared-dishes-and-using-low-sodium-seasonings-on-sodium-intake-reduction
#9
Misako Nakadate, Junko Ishihara, Motoki Iwasaki, Kaori Kitamura, Erika Kato, Junta Tanaka, Kazutoshi Nakamura, Takuma Ishihara, Ayumi Shintani, Ribeka Takachi
BACKGROUND/OBJECTIVES: Objective methods such as the monitoring of salt concentrations in home-prepared dishes may be effective in reducing salt intake. We investigated the effect of monitoring the salt concentration of home-prepared dishes (Monitoring) on salt reduction and change in taste threshold, and the effect of the simultaneous use of low-sodium seasonings (Seasoning) to compare the effect of Monitoring with the conventional method. SUBJECTS/METHODS: We conducted a double-blind randomized controlled study using a 2 × 2 factorial design with two interventions...
January 10, 2018: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29321630/engineered-mesenchymal-stem-cell-sheets-patches-prevents-postoperative-pancreatic-leakage-in-a-rat-model
#10
Seong-Ryong Kim, Hye-Jin Yi, Yu Na Lee, Ji Yoon Park, Robert M Hoffman, Teruo Okano, In Kyong Shim, Song Cheol Kim
Post-operative pancreatic fistula (POPF) following pancreatic resection is a life-threatening surgical complication. Cell sheets were prepared and harvested using temperature-responsive culture dishes and transplanted as patches to seal POPF. Two different mesenchymal stem cell (MSC) sheets were compared in terms of the preventative ability for pancreatic leakage in a rat model. Both rat adipose-derived stem cell (rADSC) and bone marrow-derived stem cell (rBMSC) sheets were transplanted. Those rADSC and rBMSC sheets are created without enzymes and thus maintained their cell-cell junctions and adhesion proteins with intact fibronectin on the basal side, as well as characteristics of MSCs...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29320769/electronic-nose-in-the-detection-of-wound-infection-bacteria-from-bacterial-cultures-a-proof-of-principle-study
#11
Taavi Saviauk, Juha P Kiiski, Maarit K Nieminen, Nelly N Tamminen, Antti N Roine, Pekka S Kumpulainen, Lauri J Hokkinen, Markus T Karjalainen, Risto E Vuento, Janne J Aittoniemi, Terho J Lehtimäki, Niku K Oksala
BACKGROUND: Soft tissue infections, including postoperative wound infections, result in a significant burden for modern society. Rapid diagnosis of wound infections is based on bacterial stains, cultures, and polymerase chain reaction assays, and the results are available earliest after several hours, but more often not until days after. Therefore, antibiotic treatment is often administered empirically without a specific diagnosis. METHODS: We employed our electronic nose (eNose) system for this proof-of-concept study, aiming to differentiate the most relevant bacteria causing wound infections utilizing a set of clinical bacterial cultures on identical blood culture dishes, and established bacterial lines from the gaseous headspace...
January 10, 2018: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
https://www.readbyqxmd.com/read/29318991/in-vitro-investigation-of-the-pediculicidal-activities-of-the-volatile-oil-components-of-some-medical-plants-raised-in-turkey
#12
M Emin Limoncu, Cüneyt Balcıoğlu, Tuğba Oyur, Gizem Zeybek, Ulvi Zeybek
OBJECTIVE: The human head louse Pediculus capitis has recently acquired resistance to commercially available insecticides, which has expanded the search concerning the pediculicidal activities of some herbal products. The present study aimed to assess the in vitro pediculicidal activities of volatile oils extracted from 10 medical plants raised in Turkey: Rosa damascena (red provins rose), Pelargonium graveolens (geranium), Lavandula angustifolia (lavender), Salvia triloba (salvia), Rosmarinus officinalis (rosemary; two different chemotypes), Citrus bergamia (citrus tree), Cymbopogon nardus (citronella), Citrus limonum (lemon), and Cymbopogon flexuosus (lemongrass)...
December 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29314024/and-the-dish-ran-away-with-the-spoon-a-hodgepodge-of-reflections-as-rigorous-as-they-are-rambling-on-the-true-subject
#13
Greg Mogenson
In this article, the topic of 'the true subject' is explored in terms of a dialectical mode thinking which is in keeping with Jung's great insight concerning psychology's lack of an Archimedean vantage-point distinct from its topics and subject matters. Evocative and expository, the emphasis is upon the performative poetics of such thinking within the sphere of our soul-making. The interpretative mode of practice presented in the article is richly illustrated with examples from Biblical texts, literary images, and proverbial sayings, as well as from the sur-real of nursery rhyme and the genre of the murder mystery...
February 2018: Journal of Analytical Psychology
https://www.readbyqxmd.com/read/29313704/fetal-bovine-serum-fbs-a-pain-in-the-dish
#14
Jan van der Valk, Gerhard Gstraunthaler
The use of Fetal Bovine Serum in replacement alternative methods is associated with serious animal welfare concerns, as well as worrying reproducibility issues.
December 2017: Alternatives to Laboratory Animals: ATLA
https://www.readbyqxmd.com/read/29310794/cell-derived-decellularized-extracellular-matrices
#15
Greg M Harris, Irene Raitman, Jean E Schwarzbauer
The ability to create cell-derived decellularized matrices in a dish gives researchers the opportunity to possess a bioactive, biocompatible material made up of fibrillar proteins and other factors that recapitulates key features of the native structure and composition of in vivo microenvironments. By using cells in a culture system to provide a natural ECM, decellularization allows for a high degree of customization through the introduction of selected proteins and soluble factors. The culture system, culture medium, cell types, and physical environments can be varied to provide specialized ECMs for wide-ranging applications to study cell-ECM signaling, cell migration, cell differentiation, and tissue engineering purposes...
2018: Methods in Cell Biology
https://www.readbyqxmd.com/read/29306339/differences-in-nutrient-and-energy-contents-of-commonly-consumed-dishes-prepared-in-restaurants-v-at-home-in-hunan-province-china
#16
Xiaofang Jia, Jiawu Liu, Bo Chen, Donghui Jin, Zhongxi Fu, Huilin Liu, Shufa Du, Barry M Popkin, Michelle A Mendez
OBJECTIVE: Eating away from home is associated with poor diet quality, in part due to less healthy food choices and larger portions. However, few studies account for the potential additional contribution of differences in food composition between restaurant- and home-prepared dishes. The present study aimed to investigate differences in nutrients of dishes prepared in restaurants v. at home. DESIGN: Eight commonly consumed dishes were collected in twenty of each of the following types of locations: small and large restaurants, and urban and rural households...
January 8, 2018: Public Health Nutrition
https://www.readbyqxmd.com/read/29305659/development-of-micro-mechanical-device-having-two-dimensional-array-of-micro-chambers-for-cell-stretching
#17
K Minami, T Hayashi, K Sato, T Nakahara
This paper presents a novel cell stretching micro device having two-dimensional array of micro chambers. It enables an in situ time-lapse observation of stretched cell by using an optical microscope with high measurement efficiency. The presented device consists of a cell culture dish and the array of micro chambers made of silicone elastomer and extension structures made of photocurable resin, and is fabricated with MEMS technology. The fabrication process of the thin micro chamber array combines photoresist mold and lift-off process based on conventional photolithography...
January 5, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29305184/bactericidal-effect-of-silver-nanoparticles-against-the-propagation-of-clavibacter-michiganensis-infection-in-lycopersicon-esculentum-mill
#18
Raymundo Rene Rivas-Cáceres, Jose LuisStephano-Hornedo, Lugo Jorge, Vaca Rocio, Pedro Del Aguila, Gustavo Yañez-Ocampo, Martha Elena Mora-Herrera, Luis Miguel Camacho Díaz, Moisés Cipriano-Salazar, Peter Adeniyi Alaba
This study explored the use of silver nanoparticle as a bactericidal against the propagation of Clavibacter michiganensis onto tomatoes (Lycopersicon esculentum Mill). In Mexico, tomato production covers about 73% of the total vegetable production but it is affected by outbreak of bacteria canker caused by Clavibacter michiganensis subspecies michiganensis (Cmm). Silver ions are possess inhibitor properties, bactericides and high specter antimicrobials. In this study, 6 groups of culture were prepared using 6 different petri dishes where silver nanoparticles of varying concentrations (120, 84, 48, 24, 12 and 0 μg) were added...
January 2, 2018: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29304862/cluster-randomized-controlled-trial-of-a-mobile-market-intervention-to-increase-fruit-and-vegetable-intake-among-adults-in-lower-income-communities-in-north-carolina
#19
Lucia A Leone, Gina L Tripicchio, Lindsey Haynes-Maslow, Jared McGuirt, Jacqueline S Grady Smith, Janelle Armstrong-Brown, Ziya Gizlice, Alice Ammerman
BACKGROUND: Poorer diets and subsequent higher rates of chronic disease among lower-income individuals may be partially attributed to reduced access to fresh fruits and vegetables (F&V) and other healthy foods. Mobile markets are an increasingly popular method for providing access to F&V in underserved communities, but evaluation efforts are limited. The purpose of this study was to determine the impact of Veggie Van (VV), a mobile produce market, on F&V intake in lower-income communities using a group randomized controlled trial...
January 5, 2018: International Journal of Behavioral Nutrition and Physical Activity
https://www.readbyqxmd.com/read/29304256/dynamics-ultrastructure-and-gene-expression-of-human-in-vitro-organized-testis-cells-from-testicular-sperm-extraction-biopsies
#20
Kathrein von Kopylow, Wolfgang Schulze, Andrea Salzbrunn, Matthias Schaks, Elke Schäfer, Beate Roth, Stefan Schlatt, Andrej-Nikolai Spiess
STUDY QUESTION: Is it possible to induce in-vitro reorganization of primary human testis cells from testicular sperm extraction (TESE) biopsies, maintain their long-term cultivation in a 2D system and identify cellular compositions? SUMMARY ANSWER: In-vitro reorganization of primary human testis cells from TESE biopsies and their long-term cultivation on uncoated cell culture dishes is feasible and the cellular compositions can be uncovered through gene expression and microscopic analyses...
January 3, 2018: Molecular Human Reproduction
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