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https://www.readbyqxmd.com/read/28928500/a-rare-case-of-eagle-syndrome-and-diffuse-idiopathic-skeletal-hyperostosis-in-the-cervical-spine
#1
Peter C Emary, Marshall Dornink, John A Taylor
Published case studies involving diffuse idiopathic skeletal hyperostosis (DISH) along with symptomatic ossification of the stylohyoid ligament, or "Eagle syndrome," are rare. In this report, we document a case of bilateral Eagle syndrome and advanced DISH in the cervical spine of an 80-year-old man who presented with severe neck stiffness and intermittent asphyxia. The key imaging and clinical features of this disorder are also described.
August 2017: Journal of the Canadian Chiropractic Association
https://www.readbyqxmd.com/read/28926965/glycemic-responses-glycemic-index-and-glycemic-load-values-of-some-street-foods-prepared-from-plantain-musa-spp-aab-genome-in-c%C3%A3-te-d-ivoire
#2
Camille Adam Kouamé, Nestor Kouakou Kouassi, Denis Yao N'dri, Kingsley Kwadwo Asare Pereko, Maria Cristina Casiraghi, Abodo Jacko Rhedoor, Georges N'guessan Amani
The glycemic index (GI) and glycemic load (GL) of four culinary preferences including five local street dishes prepared from three varieties of plantain at different maturity stages was determined. The GI was obtained following ISO/FDI 26642:2010 protocol, and the GL was calculated from test foods' GI, considering the amount of available carbohydrate in the traditional portion size. GI values were 44 for Klaclo (with Ameletiha variety at all black stage), 39 for Aloco (with Agnrin variety at full yellow stage), 39 for Aloco (with Agnrin variety at full yellow with black spots stage); 45 for Chips (with Ameletiha variety at green stage) and 89 for Banane braisée (with Afoto variety at light green stage)...
September 16, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28923873/an-anti-persister-strategy-for-the-treatment-of-chronic-pseudomonas-aeruginosa-infections
#3
Martina Koeva, Alina D Gutu, Wesley Hebert, Jeffrey D Wager, Lael M Yonker, George A O'Toole, Frederick M Ausubel, Samuel M Moskowitz, Diane Joseph-McCarthy
Bacterial persisters are a quasi-dormant sub-population of cells that are tolerant to antibiotic treatment. The combination of the aminoglycoside tobramycin with fumarate as an antibacterial potentiator utilizes an anti-persister strategy that is aimed at reducing recurrent Pseudomonas aeruginosa infections by enhancing the killing of P. aeruginosa persisters. Stationary phase cultures of P. aeruginosa were used to generate persister cells. A range of tobramycin concentrations was tested with a range of metabolite concentrations to determine the potentiation effect of the metabolite under a variety of conditions, including a range of pH values and in the presence of azithromycin or cystic fibrosis (CF) patient sputum...
September 18, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28919455/is-there-a-role-for-il-17-in-the-pathogenesis-of-systemic-sclerosis
#4
REVIEW
Carlo Chizzolini, Aleksandra Maria Dufour, Nicolò Costantino Brembilla
In systemic sclerosis (SSc) immuno-inflammatory events are central to disease development. Amongst other mediators of inflammation, interleukin 17 (IL-17) and Th17 cells have been reported to be increased in the peripheral blood and target organs including involved skin in SSc. They participate and amplify inflammatory responses by inducing the production of cytokines such as IL-6, chemokines such as CCL2 and CXCL8 (IL-8), matrix metalloproteinases-1, -2, -9 and the expression of adhesion molecules in stromal cells including fibroblasts and endothelial cells...
September 14, 2017: Immunology Letters
https://www.readbyqxmd.com/read/28918504/autism-spectrum-disorders-and-disease-modeling-using-stem-cells
#5
REVIEW
Anita Brito, Fabiele Baldino Russo, Alysson Renato Muotri, Patricia Cristina Baleeiro Beltrão-Braga
Autism spectrum disorders (ASD) represent a variety of disorders characterized as complex lifelong neurodevelopment disabilities, which may affect the ability of communication and socialization, including typical comportments like repetitive and stereotyped behavior. Other comorbidities are usually present, such as echolalia, hypotonia, intellectual disability and difficulties in processing figured speech. Furthermore, some ASD individuals may present certain abilities, such as eidetic memory, outstanding musical or painting talents and special mathematical skills, among others...
September 16, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28917809/characterization-of-sodium-tripolyphosphate-and-sodium-citrate-dehydrate-residues-on-surfaces
#6
M Sadi Gurses, Can Erkey, Seda Kizilel, Alper Uzun
Sodium tripolyphosphate (STPP) and sodium citrate dihydrate (sodium citrate) are the most widely used components in detergent formulations. Here, we characterized these two components on glass surfaces to assess their possible exposures from white spots on dishwasher-washed dishes. Ultraviolet/visible near infrared spectroscopy (UV/Vis-NIR), Fourier transform infrared spectroscopy (FTIR) in the attenuated total reflectance mode (ATR-FTIR), Raman spectroscopy and laser ablation-inductively coupled plasma mass spectroscopy (LA-ICP-MS) were utilized to design calibration models for a range of STPP and sodium citrate concentrations 1-8% w/w) precipitated on glass surfaces...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28899452/lc-hrms-for-characterizing-durum-wheat-pasta-production-variability-and-consumer-overall-liking
#7
Francesca Lambertini, Daniele Cavanna, Dante Catellani, Mario Li Vigni, Caterina Durante, Alessandro D'Alessandro, Michele Suman
Semolina pasta represents one of the most important dishes in Italian cuisine worldwide. Italy is the leader in its production and, recently, the worldwide diffusion of its production has begun to grow tremendously. The perceived quality of a food product, such as pasta, is a key feature that allows a company to increase and maintain the competitive advantage of a specific brand. The overall flavor perception of the consumer, therefore, has become as important as other key quality factors such as texture and color; thus, the food industry needs to meet consumer expectations and needs the tools to objectively “measure” the quality of food products...
September 12, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28895536/impact-of-kitchen-organization-on-oral-intake-of-malnourished-inpatients-a-two-center-study
#8
Alicia Calleja-Fernández, Cristina Velasco-Gimeno, Alfonso Vidal-Casariego, Begoña Pintor-de-la-Maza, Laura Frías-Soriano, Rocío Villar-Taibo, Pilar García-Peris, Isidoro Cano-Rodríguez, Camino García-Fernández, María D Ballesteros-Pomar
AIM: To determine the impact of the type of hospital kitchen on the dietary intake of patients. METHODS: A cross-sectional, two-centre study, of cooking in a traditional kitchen (TK) and in a chilled kitchen (CK). Subjective global assessment (SGA) was used for nutritional diagnosis. Before study start, a dietician performed a nutritional assessment of the menus of each hospital. All dishes were weighed upon arrival to the ward and at the end of the meal. RESULTS: 201 and 41 patients from the centres with TK and CK respectively were evaluated...
October 2017: Endocrinología, diabetes y nutrición
https://www.readbyqxmd.com/read/28890514/attack-rates-of-norovirus-and-campylobacter-food-poisonings-that-cannot-be-represented-by-numeric-constant-but-by-plotting-attack-rate-against-number-of-patients-per-incident-in-cartesian-coordinates
#9
Hiroshi Yoshikura, Fumihiko Takeuchi
 In norovirus and Campylobacter food poisonings, the frequencies of the number of patients per incident and that of the number of eaters per incident followed lognormal distribution, and their respective medians were 12~27 and 23~48 for norovirus food poisoning and 5~8 and 9~21 for Campylobacter food poisoning. The lognormal frequency distribution of eaters could be simulated by assuming that people are attracted more to a dish that has already attracted many. The number of patients per incident and that of eaters per incident were not necessarily inter-correlated; the frequencies of the attack rates (number of patients/number of eaters) were distributed evenly from ~0...
September 11, 2017: Japanese Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28890327/induction-of-interdigitating-cell-processes-in-podocyte-culture
#10
Eishin Yaoita, Yutaka Yoshida, Masaaki Nameta, Hiroki Takimoto, Hidehiko Fujinaka
Highly organized cell processes characterize glomerular podocytes in vivo. However, podocytes in culture have a simple morphology lacking cell processes, especially upon reaching confluence. Here, we aimed to establish culture conditions under which cultured podocytes extend cell processes at confluence. Among various culture conditions that could possibly cause phenotypic changes in podocytes, we examined the effects of heparin, all-trans retinoic acid, fetal bovine serum, and extracellular matrices on the morphology of podocytes in rat primary culture...
September 7, 2017: Kidney International
https://www.readbyqxmd.com/read/28889351/multidimensional-phenotyping-of-breast-cancer-cell-lines-to-guide-preclinical-research
#11
Jodi M Saunus, Chanel E Smart, Jamie R Kutasovic, Rebecca L Johnston, Priyakshi Kalita-de Croft, Mariska Miranda, Esdy N Rozali, Ana Cristina Vargas, Lynne E Reid, Eva Lorsy, Sibylle Cocciardi, Tatjana Seidens, Amy E McCart Reed, Andrew J Dalley, Leesa F Wockner, Julie Johnson, Debina Sarkar, Marjan E Askarian-Amiri, Peter T Simpson, Kum Kum Khanna, Georgia Chenevix-Trench, Fares Al-Ejeh, Sunil R Lakhani
PURPOSE: Cell lines are extremely useful tools in breast cancer research. Their key benefits include a high degree of control over experimental variables and reproducibility. However, the advantages must be balanced against the limitations of modelling such a complex disease in vitro. Informed selection of cell line(s) for a given experiment now requires essential knowledge about molecular and phenotypic context in the culture dish. METHODS: We performed multidimensional profiling of 36 widely used breast cancer cell lines that were cultured under standardised conditions...
September 9, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28887969/viability-of-hek-293-cells-on-poly-%C3%AE-hydroxybutyrate-phb-biosynthesized-from-a-mutant-azotobacter-vinelandii-strain-cast-film-and-electrospun-scaffolds
#12
Angel Romo-Uribe, Angelica Meneses-Acosta, Maraolina Domínguez-Díaz
Sterilization, cytotoxicity and cell viability are essential properties defining a material for medical applications and these characteristics were investigated for poly(β-hydroxybutyrate) (PHB) of 230kDa obtained by bacterial synthesis from a mutant strain of Azotobacter vinelandii. Cell viability was investigated for two types of PHB scaffolds, solution cast films and non-woven electrospun fibrous membranes, and the efficiency was compared against a culture dish. The biosynthesized PHB was sterilized by ultraviolet radiation and autoclave, it was found that the thermal properties and intrinsic viscosity remained unchanged indicating that the sterilization methods did not degrade the polymer...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28887108/new-approach-for-the-derivation-of-melanocytes-from-induced-pluripotent-stem-ips-cells
#13
Tamihiro Kawakami, Tatsuro Okano, Sora Takeuchi, Kayoko Osumi, Yoshinao Soma, Munenari Itoh, Tomohisa Hirobe, Kowichi Jimbow
Induced pluripotent stem (iPS) cells have the ability to differentiate into multiple cell types in the body and have an unlimited growth potential. However, iPS cell-derived melanocytes produced by existing protocols have significant limitations in developing novel strategies for regenerative medicine and cell therapies of pigmentation disorders in humans, because they involve culture in media containing fetal bovine serum (FBS) and non-physiologic agents. In this study, we established a new in vitro approach to generate iPS cell-derived human melanocytes which have higher proliferation rates and increased melanin production compared to melanocytes prepared by previously reported approaches...
September 5, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28881093/expansion-of-bone-marrow-mesenchymal-stromal-cells-in-perfused-3d-ceramic-scaffolds-enhances-in-vivo-bone-formation
#14
Allison I Hoch, Ralph Duhr, Nunzia Di Maggio, Arne Mehrkens, Marcel Jakob, David Wendt
Bone marrow-derived mesenchymal stromal cells (BMSC), when expanded directly within 3D ceramic scaffolds in perfusion bioreactors, more reproducibly form bone when implanted in vivo as compared to conventional expansion on 2D polystyrene dishes/flasks. Since the bioreactor-based expansion on 3D ceramic scaffolds encompasses multiple aspects that are inherently different from expansion on 2D polystyrene, we aimed to decouple the effects of specific parameters among these two model systems. We assessed the effects of the: i) 3D scaffold vs 2D surface, ii) ceramic vs polystyrene materials, and iii) BMSC niche established within the ceramic pores during in vitro culture, on subsequent in vivo bone formation...
September 7, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28880277/use-of-a-neonatal-rat-system-as-a-bioincubator-to-generate-adult-like-mature-cardiomyocytes-from-human-and-mouse-pluripotent-stem-cells
#15
Gun-Sik Cho, Emmanouil Tampakakis, Peter Andersen, Chulan Kwon
Pluripotent stem cells (PSCs), including induced PSCs, hold great potential for personalized disease modeling, drug testing and cell-based therapeutics. However, cells differentiated from PSCs remain immature in a dish, and thus there are serious caveats to their use in modeling adult-onset diseases such as cardiomyopathies and Alzheimer's disease. By taking advantage of knowledge gained about mammalian development and from bioinformatics analyses, we recently developed a neonatal rat system that enables maturation of PSC-derived cardiomyocytes into cardiomyocytes analogous to those seen in adult animals...
October 2017: Nature Protocols
https://www.readbyqxmd.com/read/28880251/studying-electrotaxis-in-microfluidic-devices
#16
REVIEW
Yung-Shin Sun
Collective cell migration is important in various physiological processes such as morphogenesis, cancer metastasis and cell regeneration. Such migration can be induced and guided by different chemical and physical cues. Electrotaxis, referring to the directional migration of adherent cells under stimulus of electric fields, is believed to be highly involved in the wound-healing process. Electrotactic experiments are conventionally conducted in Petri dishes or cover glasses wherein cells are cultured and electric fields are applied...
September 7, 2017: Sensors
https://www.readbyqxmd.com/read/28879677/transcriptomic-analysis-of-gingival-mesenchymal-stem-cells-cultured-on-3d-bioprinted-scaffold-a-promising-strategy-for-neuroregeneration
#17
Agnese Gugliandolo, Francesca Diomede, Paolo Cardelli, Alessia Bramanti, Domenico Scionti, Placido Bramanti, Oriana Trubiani, Emanuela Mazzon
The combined approach of Mesenchymal stem cells (MSCs) and scaffolds has been proposed as a potential therapeutic tool for the treatment of neurodegenerative diseases. Indeed, even if MSCs can promote neuronal regeneration, replacing lost neurons or secreting neurotrophic factors, many limitations still exist for their application in regenerative medicine, including the low survival and differentiation rate. The scaffolds, by mimicking the endogenous microenvironment, have shown to promote cell survival, proliferation and differentiation...
September 6, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28877248/flying-foxes-create-extensive-seed-shadows-and-enhance-germination-success-of-pioneer-plant-species-in-deforested-madagascan-landscapes
#18
Ryszard Oleksy, Luca Giuggioli, Thomas J McKetterick, Paul A Racey, Gareth Jones
Seed dispersal plays a significant role in forest regeneration and maintenance. Flying foxes are often posited as effective long-distance seed dispersers due to their large home ranges and ability to disperse seeds when flying. We evaluate the importance of the Madagascan flying fox Pteropus rufus in the maintenance and regeneration of forests in one of the world's priority conservation areas. We tested germination success of over 20,000 seeds from the figs Ficus polita, F. grevei and F. lutea extracted from bat faeces and ripe fruits under progressively more natural conditions, ranging from petri-dishes to outdoor environments...
2017: PloS One
https://www.readbyqxmd.com/read/28868245/isolation-culture-and-characterization-of-human-sertoli-cells-by-flow-cytometry-development-of-procedure
#19
Mohammad Reza Lakpour, Samaneh Aghajanpour, Morteza Koruji, Abdolhossein Shahverdi, Mohammad Ali Sadighi-Gilani, Marjan Sabbaghian, Reza Aflatoonian, Majid Rajabian-Naghandar
BACKGROUND: The sertoli cells in the testis create unique and safe environment to protect seminiferous tubules from auto antigens and invading pathogens. These cells produce the survival factor of the blood-testis barrier and produce special materials such as androgen binding proteins and contribute to the coordinated action of spermatogenesis. Given that the sertoli cells play an essential role in spermatogenesis and the lack of these cells leads to the disruption of spermatogenesis, it is necessary to investigate the behavior and performance of these cells...
April 2017: Journal of Reproduction & Infertility
https://www.readbyqxmd.com/read/28863479/ag-fractal-structures-in-electroless-metal-deposition-systems-with-and-without-magnetic-field
#20
Huria Ibrahim, Hiba Farah, Amal Zein Eddin, Samih Isber, Rabih Sultan
Metal electrodeposition systems display tree-like structures with extensive ramification and a fractal character. Electrolysis is not a necessary route for the growth of such dendritic metal deposits. We can grow beautiful ramification patterns via a simple redox reaction. We present here a study of silver (Ag) deposits from the reduction of Ag(+) in (AgNO3) solution by metallic copper. The experiments are carried out in discotic geometry, in a Petri dish hosting a thin AgNO3 solution film. A variety of deposited structures and patterns is obtained at different Ag(+) concentrations, yet with essentially the same fractal dimension averaged at 1...
August 2017: Chaos
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