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https://www.readbyqxmd.com/read/29145019/ordered-cylindrical-micropatterned-petri-dishes-used-as-scaffolds-for-cell-growth
#1
Van-Tien Bui, Le Thi Thuy, Joon Sig Choi, Ho-Suk Choi
Three-dimensional (3D) culture dish patterned with a microwell structure demonstrates a great application potential in biotechnology. This study reports on the easy fabrication of an ordered customizable honeycomb microwell array on the surface of polymer substrates including the commercial Petri dish to create a biological platform for cell culture. The fabrication method is based on a very simple solvent dip-coating technique and the methanol accumulation-induced phase separation in which a binary mixture of chloroform and methanol is used to induce a ternary solution and to guarantee the formation of the ordered pore array on the substrate...
November 10, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29142327/the-circumstellar-envelope-around-the-s-type-agb-star-w-aql-effects-of-an-eccentric-binary-orbit
#2
S Ramstedt, S Mohamed, W H T Vlemmings, T Danilovich, M Brunner, E De Beck, E M L Humphreys, M Lindqvist, M Maercker, H Olofsson, F Kerschbaum, G Quintana-Lacaci
Context: Recent observations at subarcsecond resolution, now possible also at submillimeter wavelengths, have shown intricate circumstellar structures around asymptotic giant branch (AGB) stars, mostly attributed to binary interaction. The results presented here are part of a larger project aimed at investigating the effects of a binary companion on the morphology of circumstellar envelopes (CSEs) of AGB stars. Aims: AGB stars are characterized by intense stellar winds that build CSEs around the stars...
September 21, 2017: Astronomy and Astrophysics
https://www.readbyqxmd.com/read/29140275/old-fashioned-vs-ultra-processed-based-current-diets-possible-implication-in-the-increased-susceptibility-to-type-1-diabetes-and-celiac-disease-in-childhood
#3
REVIEW
Sandra V Aguayo-Patrón, Ana M Calderón de la Barca
Ultra-processed foods are ready-to-heat and ready-to-eat products created to replace traditional homemade meals and dishes due to convenience and accessibility. Because of their low-fiber and high-fat and sugar composition, these foodstuffs could induce a negative impact on health. They are partially responsible for obesity and chronic non-transmissible diseases; additionally, they could impact in the prevalence of autoimmune diseases such as type 1 diabetes and celiac disease. The rationale is that the nutritional composition of ultra-processed foodstuffs can induce gut dysbiosis, promoting a pro-inflammatory response and consequently, a "leaky gut"...
November 15, 2017: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/29137641/improving-e-coli-growth-performance-by-manipulating-small-rna-expression
#4
REVIEW
Alejandro Negrete, Joseph Shiloach
Efficient growth of E. coli, especially for production of recombinant proteins, has been a challenge for the biotechnological industry since the early 1970s. By employing multiple approaches, such as different media composition, various growth strategies and specific genetic manipulations, it is now possible to grow bacteria to concentrations exceeding 100 g/L and to achieve high concentrations of recombinant proteins. Although the growth conditions are carefully monitored and maintained, it is likely that during the growth process cells are exposed to periodic stress conditions, created by fluctuations in pH, dissolved oxygen, temperature, glucose, and salt concentration...
November 14, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29136882/growth-of-escherichia-coli-on-the-gaas-001-surface
#5
Elnaz Nazemi, Walid M Hassen, Eric H Frost, Jan J Dubowski
Detection of pathogenic bacteria and monitoring their susceptibility to antibiotics are of great importance in the fields of medicine, pharmaceutical research, as well as water and food industries. In order to develop a photonic biosensor for detection of bacteria by taking advantage of photoluminescence (PL) of GaAs-based devices, we have investigated the capture and growth of Escherichia coli K12 on bare and biofunctionalized surfaces of GaAs (001) - a material of interest for capping different semiconductor microstructures...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136040/morphological-adaptations-in-breast-cancer-cells-as-a-function-of-prolonged-passaging-on-compliant-substrates
#6
Sana Syed, Joseph Schober, Alexandra Blanco, Silviya Petrova Zustiak
Standard tissue culture practices involve propagating cells on tissue culture polystyrene (TCP) dishes, which are flat, 2-dimensional (2D) and orders of magnitude stiffer than most tissues in the body. Such simplified conditions lead to phenotypical cell changes and altered cell behaviors. Hence, much research has been focused on developing novel biomaterials and culture conditions that more closely emulate in vivo cell microenvironments. In particular, biomaterial stiffness has emerged as a key property that greatly affects cell behaviors such as adhesion, morphology, proliferation and motility among others...
2017: PloS One
https://www.readbyqxmd.com/read/29133187/duddingtonia-flagrans-formulated-in-rice-bran-in-the-control-of-oesophagostomum-spp-intestinal-parasite-of-swine
#7
João Victor Facchini Rodrigues, Fabio Ribeiro Braga, Artur Kanadani Campos, Lorendane Millena de Carvalho, Juliana Milani Araujo, Anderson Rocha Aguiar, Carolina Magri Ferraz, Wendeo Ferreira da Silveira, Marisa Caixetta Valadão, Thais de Oliveira, Samuel Galvão de Freitas, Jackson Victor de Araújo
Three experimental assays with Duddingtonia flagrans (isolated AC001) were carried out. The growth of the genus Duddingtonia present in formulation of rice bran, its predatory capability on Oesophagostomum spp. infective larvae (L3) in petri dishes (assay 1), its action in faecal cultures with eggs of that parasite (assay 2) and isolate's capability of predation after passing through gastrointestinal tract of swine (assay 3) was evaluated. At assay 3, feces were collected at time intervals of 12, 24, 36, 48, and 60 h after feed animals with the formulation...
November 10, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/29132810/medium-retaining-petri-dish-insert-to-grow-and-image-cultured-cells
#8
Lech Kiedrowski, Alan Feinerman
BACKGROUND: Microscope chambers that accept glass coverslips with cultured cells are often used to monitor intracellular Ca(2+) concentration ([Ca(2+)]i) during cell superfusion. Unfortunately, the experimental maneuvers associated with the coverslip installation in these chambers (medium removal and re-application) trigger unintended [Ca(2+)]i elevations. NEW METHOD: To prevent these [Ca(2+)]i elevations, a Petri dish insert has been constructed. The insert features a superfusion-optimized well to grow cell cultures...
November 10, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/29127711/can-i-serve-this-dish-half-baked-approaches-to-publishing-some-less-than-perfect-research
#9
EDITORIAL
Margaret H Kearney
No abstract text is available yet for this article.
November 11, 2017: Research in Nursing & Health
https://www.readbyqxmd.com/read/29127646/can-we-spice-up-our-christmas-dinner-busting-the-myth-of-the-chinese-restaurant-syndrome
#10
N W E van den Berg, J Neefs, W R Berger, S W E Baalman, E Meulendijks, M Kawasaki, E M Kemper, F R Piersma, M W Veldkamp, R Wesselink, S P J Krul, J R de Groot
BACKGROUND: Monosodium glutamate (MSG), also referred to as Vetsin or E621, is a flavour enhancer frequently used in Asian cuisine and abundantly present in the famous Chinese dish Peking duck. MSG is notorious for triggering the onset of the so-called 'Chinese restaurant syndrome' (CRS), a complex of unpleasant symptoms, which might include flushing, sweating and the onset of atrial fibrillation (AF). This study aims to determine the effects of MSG on the occurrence of AF. METHODS: We conducted a placebo self-controlled single-arm study in the Academic Medical Centre in Amsterdam...
November 10, 2017: Netherlands Heart Journal
https://www.readbyqxmd.com/read/29121079/challenges-and-opportunities-for-moringa-growers-in-southern-ethiopia-and-kenya
#11
Diriba B Kumssa, Edward J M Joy, Scott D Young, David W Odee, E Louise Ander, Charles Magare, James Gitu, Martin R Broadley
Moringa oleifera (MO) and M. stenopetala (MS) are two commonly cultivated species of the Moringaceae family. Some households in southern Ethiopia (S. ETH) and Kenya (KEN) plant MS and MO, respectively. The edible parts of these species are rich in amino acids, vitamins and minerals, especially selenium. Despite their nutritional value, Moringa is sometimes considered as a "famine food". The aim of this study was to determine the extent of dietary utilization of these plants by Moringa Growing Households (MGHs)...
2017: PloS One
https://www.readbyqxmd.com/read/29120400/fibrin-enhanced-canonical-wnt-signaling-directs-plasminogen-expression-in-cementoblasts
#12
Saeed Ur Rahman, Chan Ho Park, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Cementum is a mineralized layer on the tooth's root surface and facilitates the biomechanical anchoring of fibrous connective tissues as a part of tooth-supportive complexes. Previously, we observed that OCCM30 cementoblasts cultured on fibrin matrices underwent apoptosis due to fibrin degradation through the expression of proteases. Here, we demonstrated that OCCM30 on fibrin matrices (OCCM30-fibrin) enhanced canonical Wnt signaling, which directed to plasminogen expression. The OCCM30-fibrin showed higher levels of Wnt3a expression, nuclear translocation of β-catenin, and T-cell factor (TCF) optimal motif (TOP) reporter activity than the cells on tissue culture dishes (OCCM30-TCD), indicating that the OCCM30-fibrin enhanced canonical Wnt/β-catenin signaling...
November 9, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29119512/multi-agent-simulations-of-population-behavior-a-promising-tool-for-systems-biology
#13
Alfredo Colosimo
This contribution reports on the simulation of some dynamical events observed in the collective behavior of different kinds of populations, ranging from shape-changing cells in a Petri dish to functionally correlated brain areas in vivo. The unifying methodological approach, based upon a Multi-Agent Simulation (MAS) paradigm as incorporated in the NetLogo™ interpreter, is a direct consequence of the cornerstone that simple, individual actions within a population of interacting agents often give rise to complex, collective behavior...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29111152/vegetarian-or-meat-food-choice-modeling-of-main-dishes-occurs-outside-of-awareness
#14
Chelsea D Christie, Frances S Chen
It is well established that the amount eaten by other people affects how much we eat, but unanswered questions exist regarding how much the food choices of other people affect the types of food that we choose. Past research on food choice modeling has primarily been conducted in controlled laboratory situations and has focused on snack foods. The current research examines the extent to which food choice modeling of a main dish occurs in a real-life context and whether people are aware of being influenced by others...
October 27, 2017: Appetite
https://www.readbyqxmd.com/read/29108531/eating-occasions-and-the-contribution-of-foods-to-sodium-and-potassium-intakes-in-adults
#15
Kacie M Dickinson, Lily Chan, Carly J Moores, Jacqueline Miller, Jolene Thomas, Alison Yaxley, Kathryn Jackson, Kaye Mehta, Louisa Matwiejczyk, Amanda Wray, Michelle Miller
OBJECTIVE: To examine dietary Na and K intake at eating occasions in Australian adults and identify the contribution of major food sources to Na and K at different eating occasions. DESIGN: Secondary analysis of 24 h recall diet data from the Australian Health Survey (2011-2013). SETTING: Nationally representative survey in Australia. SUBJECTS: Male and female Australians aged 18-84 years (n 7818). RESULTS: Dinner contributed the greatest proportion to total daily Na intake (33 %) and K intake (35 %)...
November 7, 2017: Public Health Nutrition
https://www.readbyqxmd.com/read/29107681/myogenic-progenitor-specification-from-pluripotent-stem-cells
#16
REVIEW
Alessandro Magli, Rita Rc Perlingeiro
Pluripotent stem cells represent important tools for both basic and translational science as they enable to study mechanisms of development, model diseases in vitro and provide a potential source of tissue-specific progenitors for cell therapy. Concomitantly with the increasing knowledge of the molecular mechanisms behind activation of the skeletal myogenic program during embryonic development, novel findings in the stem cell field provided the opportunity to begin recapitulating in vitro the events occurring during specification of the myogenic lineage...
October 28, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/29104755/combined-approach-for-finding-susceptibility-genes-in-dish-chondrocalcinosis-families-whole-genome-wide-linkage-and-ibs-ibd-studies
#17
Ana Rita Couto, Bruna Parreira, Russell Thomson, Marta Soares, Deborah M Power, Jim Stankovich, Jácome Bruges Armas, Matthew A Brown
Twelve families with exuberant and early-onset calcium pyrophosphate dehydrate chondrocalcinosis (CC) and diffuse idiopathic skeletal hyperostosis (DISH), hereafter designated DISH/CC, were identified in Terceira Island, the Azores, Portugal. Ninety-two (92) individuals from these families were selected for whole-genome-wide linkage analysis. An identity-by-descent (IBD) analysis was performed in 10 individuals from 5 of the investigated pedigrees. The chromosome area with the maximal logarithm of the odds score (1...
2017: Human Genome Variation
https://www.readbyqxmd.com/read/29102930/re-evaluation-of-culture-condition-of-pc12-and-sh-sy5y-cells-based-on-growth-rate-and-amino-acid-consumption
#18
Hiroshi Sakagami, Ryuichiro Suzuki, Yoshiaki Shirataki, Soichi Iwama, Mika Nakagawa, Hayato Suzuki, Kenta Tanaka, Nobuaki Tamura, Hiroshi Takeshima
BACKGROUND/AIM: Most of the previous investigators have used various types of media for the culture of nerve cells. In order to optimize the culture conditions, we compared the growth rate and amino acid consumption by two popular neuron models, rat PC12 and human SH-SY5Y, grown in DMEM or DMEM: Ham's F-12 (1:1): non-essential amino acids, supplemented with 10% fetal bovine serum (referred to DMEM and Mix, respectively). MATERIALS AND METHODS: Cell growth was monitored by the MTT method...
November 2017: In Vivo
https://www.readbyqxmd.com/read/29102668/lead-exposure-in-young-children-over-a-5-year-period-from-urban-environments-using-alternative-exposure-measures-with-the-us-epa-ieubk-model-a-trial
#19
Brian Gulson, Alan Taylor, Marc Stifelman
The US Environmental Protection Agency (EPA) Integrated Exposure Uptake Biokinetic (IEUBK) model has been widely used to predict blood lead (PbB) levels in children especially around industrial sites. Exposure variables have strongly focussed on the major contribution of lead (Pb) in soil and interior dust to total intake and, in many studies, site-specific data for air, water, diet and measured PbB were not available. We have applied the IEUBK model to a comprehensive data set, including measured PbB, for 108 children monitored over a 5-year period in Sydney, New South Wales, Australia...
November 2, 2017: Environmental Research
https://www.readbyqxmd.com/read/29098733/au-coated-honeycomb-structure-as-an-efficient-tco-free-counter-electrode-for-quantum-dot-sensitized-solar-cells
#20
Van-Duong Dao, Van-Tien Bui, Minki Baek, The-Long Phan, Kijung Yong, Ho-Suk Choi
This study reports the fabrication of a Petri dish patterned with cylindrical micro cavity which are produced using a one-step solvent-immersion phase-separation process. The developed 3D honeycomb Petri is coated with Au film through a sputtering method as an efficient Au-coated FTO-free electrode for quantum-dot-sensitized solar cells. Due to the high specific active surface area of the electrode with the Au-coated honeycomb structure, the energy conversion efficiency of devices which use this electrode is 5...
November 2, 2017: Chemistry: a European Journal
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