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https://www.readbyqxmd.com/read/29791795/surface-acoustic-wave-saw-driven-device-for-dynamic-cell-cultures
#1
Gina Greco, Matteo Agostini, Ilaria Tonazzini, Damiano Sallemi, Stefano Barone, Marco Cecchini
In the last decades new types of cell cultures have been introduced to provide better cell survival and development, and micro/nano-environmental physico-chemical conditions aimed to mimic those present in vivo. However, despite the efforts made, the systems available to date are often difficult to replicate and use. Here, an easy-to-use surface acoustic wave (SAW)-based platform is presented for realizing dynamic cell cultures that is compatible with standard optical microscopes, incubators and cell culture dishes...
May 23, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29791489/contractile-force-measurement-of-human-induced-pluripotent-stem-cell-derived-cardiac-cell-sheet-tissue
#2
Daisuke Sasaki, Katsuhisa Matsuura, Hiroyoshi Seta, Yuji Haraguchi, Teruo Okano, Tatsuya Shimizu
We have developed our original tissue engineering technology "cell sheet engineering" utilizing temperature-responsive culture dishes. The cells are confluently grown on a temperature-responsive culture dish and can be harvested as a cell sheet by lowering temperature without enzymatic digestion. Cell sheets are high-cell-density tissues similar to actual living tissues, maintaining their structure and function. Based on this "cell sheet engineering", we are trying to create functional cardiac tissues from human induced pluripotent stem cells, for regenerative therapy and in vitro drug testing...
2018: PloS One
https://www.readbyqxmd.com/read/29786754/regulation-of-interferon-signaling-and-hcv%C3%A2-rna-replication-by-extracellular-matrix
#3
Takuya Kuwashiro, Shinji Iwane, Xia Jinghe, Sachiko Matsuhashi, Yuichiro Eguchi, Keizo Anzai, Kazuma Fujimoto, Toshihiko Mizuta, Naoya Sakamoto, Masanori Ikeda, Nobuyuki Kato, Iwata Ozaki
Although interferon (IFN)‑based treatment of patients with chronic hepatitis C virus (HCV) infection is widely applied, treatment resistance is often observed in patients with advanced liver fibrosis. Given that the molecular mechanisms of IFN resistance in liver fibrosis remain elusive, the present study investigated the effects of extracellular matrix (ECM) on IFN signaling in hepatic cells. The native HuH‑7 human hepatoma cell line and HuH‑7 cells were stably transfected with full‑length HCV‑RNA fused with Renilla luciferase (OR6 cells) were cultured on ECM‑coated dishes or non‑coated plastic dishes (NDs), and treated with human IFN‑α...
May 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29786548/designer-human-tissue-coming-to-a-lab-near-you
#4
David C Hay, Cliona O'Farrelly
Human pluripotent stem cells (PSCs) offer a scalable alternative to primary and transformed human tissue. PSCs include human embryonic stem cells, derived from the inner cell mass of blastocysts unsuitable for human implantation; and induced PSCs, generated by the reprogramming of somatic cells. Both cell types display the ability to self-renew and retain pluripotency, promising an unlimited supply of human somatic cells for biomedical application. A distinct advantage of using PSCs is the ability to select for genetic background, promising personalized modelling of human biology 'in a dish' or immune-matched cell-based therapies for the clinic...
July 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29783706/effect-of-temperature-changes-on-serum-protein-adsorption-on-thermoresponsive-cell-culture-surfaces-monitored-by-a-quartz-crystal-microbalance-with-dissipation
#5
Jun Kobayashi, Yoshinori Arisaka, Nobuhiko Yui, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano
Thermoresponsive cell-culture polystyrene (PS) surfaces that are grafted with poly( N -isopropylacrylamide) (PIPAAm) facilitate the cultivation of cells at 37 °C and the detachment of cultured cells as a sheet with an underlying extracellular matrix (ECM) by reducing the temperature. However, the ECM and cell detachment mechanisms are still unclear because the detachment of cells from thermoresponsive surfaces is governed by complex interactions among the cells/ECM/surface. To explore the dynamic behavior of serum protein adsorption/desorption, thermoresponsive surfaces that correspond to thermoresponsive tissue-culture PS dishes were formed on sensor chips for quartz crystal microbalance with dissipation (QCM-D) measurements...
May 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29781496/a-universal-platform-for-selection-and-high-resolution-phenotypic-screening-of-bacterial-mutants-using-the-nanowell-slide
#6
H Antypas, M Veses-Garcia, E Weibull, H Andersson-Svahn, A Richter-Dahlfors
The Petri dish and microtiter plate are the golden standard for selection and screening of bacteria in microbiological research. To improve on the limited resolution and throughput of these methods, we developed a universal, user-friendly platform for selection and high-resolution phenotypic screening based on the nanowell slide. This miniaturized platform has an optimal ratio between throughput and assay complexity, holding 672 nanowells of 500 nl each. As monoclonality is essential in bacterial genetics, we used FACS to inoculate each nanowell with a single bacterium in 15 min...
May 21, 2018: Lab on a Chip
https://www.readbyqxmd.com/read/29776365/nutrient-intake-disparities-in-the-us-modeling-the-effect-of-food-substitutions
#7
Zach Conrad, LuAnn K Johnson, James N Roemmich, WenYen Juan, Lisa Jahns
BACKGROUND: Diet quality among federal food assistance program participants remains low, and little research has assessed the diet quality of food insecure non-participants. Further research is needed to assess the extent to which food substitutions can improve the nutritional status of these vulnerable populations. Substituting egg dishes for other commonly consumed dishes at certain eating occasions may be an effective strategy for improving the daily nutrient intake among these groups...
May 17, 2018: Nutrition Journal
https://www.readbyqxmd.com/read/29774073/polypyrrole-chitosan-conductive-biomaterial-synchronizes-cardiomyocyte-contraction-and-improves-myocardial-electrical-impulse-propagation
#8
Zhi Cui, Nathan C Ni, Jun Wu, Guo-Qing Du, Sheng He, Terrence M Yau, Richard D Weisel, Hsing-Wen Sung, Ren-Ke Li
Background: The post-myocardial infarction (MI) scar interrupts electrical impulse propagation and delays regional contraction, which contributes to ventricular dysfunction. We investigated the potential of an injectable conductive biomaterial to restore scar tissue conductivity and re-establish synchronous ventricular contraction. Methods: A conductive biomaterial was generated by conjugating conductive polypyrrole (PPY) onto chitosan (CHI) backbones. Trypan blue staining of neonatal rat cardiomyocytes (CMs) cultured on biomaterials was used to evaluate the biocompatibility of the conductive biomaterials...
2018: Theranostics
https://www.readbyqxmd.com/read/29773904/methanol-fixed-fibroblasts-serve-as-feeder-cells-to-maintain-stem-cells-in-the-pluripotent-state-in-vitro
#9
Yahui Ren, Ziyu Ma, Tong Yu, Min Ling, Huayan Wang
Preparation of mouse embryonic fibroblast (MEF) feeder cells to maintain pluripotent stem cells (PSCs) is time consuming and involved in animal issues. Here, we demonstrated a novel method to prepare feeder cells with high efficiency, timesaving, and low costs. MEFs in 3 × 104 cell/cm2 were fixed by methanol for 5 min and air drying for 5 min. Thereafter, the methanol fixed MEF cells (MT-MEF) were able to be used directly to culture PSCs or stored at room temperature for the future usage. PSCs cultured on MT-MEF could be continuously expanded for over 40 passages with the naïve pluripotency...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29773134/developmental-capacity-of-ascaridia-galli-eggs-is-preserved-after-anaerobic-storage-in-faeces
#10
B Tarbiat, S Rahimian, D S Jansson, P Halvarsson, J Höglund
The reliability of the results of in vitro studies such as detection of anthelmintic resistance often depends on the ability of the parasite eggs to develop under laboratory conditions. The aim of this study was to assess the embryonation capability of the chicken roundworm Ascaridia galli eggs after storage under different conditions. Two storage media for parasite eggs were used; faeces or water. Eggs in petri dishes (90 dishes in total) containing faces or water media were first exposed either to aerobic or anaerobic conditions at different temperatures (4 °C /+O2 , 4 °C /-O2 , 25 °C/-O2 ) for a maximum of 72 days...
May 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29772696/the-effect-of-negative-pressure-wound-therapy-with-and-without-instillation-on-mature-biofilms-in-vitro
#11
Shamaila Tahir, Matthew Malone, Honghua Hu, Anand Deva, Karen Vickery
BACKGROUND: To investigate the effect of negative pressure wound therapy (NPWT) with and without instillation (NPWTi) on in vitro mature biofilm. METHODS: Mature biofilms of Pseudomonas aeruginosa and Staphylococcus aureus were grown under shear (130 rpm) on polycarbonate coupons in a CDC biofilm reactor for 3 days. Coupons containing biofilms were placed in a sterile petri dish and sealed using NPWT or NPWTi. Coupons were exposed to treatment for 24 h with NPWT alone or with instillation of: Povidone iodine solution (PVP-I) (10% w / v equivalent to 1% w / v available iodine, BETADINE® , Mundipharma, Singapore), surfactant based antimicrobial solution with polyhexamethylene biguanide (SBPHMB) (Prontosan® , B Braun Medical, Melsungen, Germany), Gentamicin 1 µg/mL (GM) (G1264 Sigma-Aldrich Pty Ltd...
May 16, 2018: Materials
https://www.readbyqxmd.com/read/29772292/does-the-cost-of-a-meal-influence-the-portion-size-effect
#12
Faris M Zuraikat, Liane S Roe, Alissa D Smethers, Levi W Reihart, Barbara J Rolls
Serving larger portions leads to increased intake, but little is known about how the cost of a meal affects this response. Therefore, we tested whether the amount of money paid for a meal influenced the portion size effect at a lunch served in a controlled restaurant-style setting. In a crossover design, 79 adults (55 women; 24 men) came to the lab once a week for 4 weeks to eat a main dish of pasta with side dishes. Across weeks, the meal was varied in two factors: portion size of the main dish (400 g or 600 g) and cost of the meal (US$8 or $16)...
May 14, 2018: Appetite
https://www.readbyqxmd.com/read/29772216/human-liver-fluke-opisthorchis-viverrini-trematoda-opisthorchiidae-in-central-myanmar-new-records-of-adults-and-metacercariae-identified-by-morphology-and-molecular-analysis
#13
Oranuch Sanpool, Win Pa Pa Aung, Rutchanee Rodpai, Wanchai Maleewong, Pewpan M Intapan
Opisthorchis-like metacercariae were found in cyprinoid fish, Puntius brevis, bought from markets in the Bago region, Central Myanmar. Adult worms recovered from experimentally-infected hamsters resembled Opisthorchis viverrini. DNA was extracted from adults and metacercariae. A portion of the mitochondrial cytochrome c oxidase subunit 1 (cox1) and the internal transcribed spacer 2 (ITS2) regions were amplified using the polymerase chain reaction and then sequenced. The sequences confirmed that the flukes were O...
May 14, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29769137/laboratory-studies-on-the-oviposition-stimuli-of-culicoides-stellifer-diptera-ceratopogonidae-a-suspected-vector-of-orbiviruses-in-the-united-states
#14
Dinesh Erram, Nathan Burkett-Cadena
BACKGROUND: Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) exert a significant impact on animal agriculture worldwide because they transmit bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) to ruminants. Without effective vaccines, BTV/EHDV vector management strategies are needed, particularly in commercial white-tailed deer (WTD) facilities. However, detailed information on the ecology of midge immatures in/around cervid operations is currently lacking...
May 16, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29768932/differential-levels-of-reactive-oxygen-species-in-murine-preadipocyte-3t3-l1-cells-cultured-on-type-i-collagen-molecule-coated-and-gel-covered-dishes-exert-opposite-effects-on-nf-%C3%AE%C2%BAb-mediated-proliferation-and-migration
#15
Xiaoling Liu, Xinyu Long, Weiwei Liu, Guodong Yao, Yeli Zhao, Toshihiko Hayashi, Shunji Hattori, Hitomi Fujisaki, Takaaki Ogura, Shin-Ichi Tashiro, Satoshi Onodera, Masayuki Yamato, Takashi Ikejima
Reactive oxygen species (ROS) participate in various cell responses in association with cell proliferation, migration, differentiation and death. Extracellular matrix (ECM) serves as cellular microenvironments for many kinds of cells, affecting cell activities. However, whether or not ECM influences cellular ROS levels has not been well studied. In this study, cells are cultured on collagen I molecule-coated (mol. coated) dishes and collagen I fibrous gel-covered (gel) dishes to explore their influence on cell behaviors...
May 16, 2018: Free Radical Research
https://www.readbyqxmd.com/read/29767686/stratification-of-mouse-vaginal-epithelium-1-development-of-3-dimensional-models-in-vitro-with-clonal-cell-lines
#16
Minako Ogawa-Tominaga, Tomohiro Umezu, Tadaaki Nakajima, Yasuhiro Tomooka
The mouse vagina consists of stratified squamous epithelium and stroma and is regulated by ovarian hormones. Vaginal epithelial cells do not stratify, but rather form a monolayer and show an inconsistent responsiveness to ovarian hormones when cultured on plastic dish or matrix. To address the discrepancy between in vivo and in vitro observations, 3 dimensional (3D) co-culture models are developed with clonal vaginal epithelial and stromal cell lines; stromal cells are embedded in collagen gel and epithelial cells are seeded on the gel...
May 14, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29766931/-in-vitro-differentiation-of-neural-cells-from-human-adipose-tissue-derived-stromal-cells
#17
Shruti D Dave, Chetan N Patel, Aruna V Vanikar, Hargovind L Trivedi
Background: Stem cells, including neural stem cells (NSCs), are endowed with self-renewal capability and hence hold great opportunity for the institution of replacement/protective therapy. We propose a method for in vitro generation of stromal cells from human adipose tissue and their differentiation into neural cells. Materials and Methods: Ten grams of donor adipose tissue was surgically resected from the abdominal wall of the human donor after the participants' informed consents...
May 2018: Neurology India
https://www.readbyqxmd.com/read/29766697/the-assessment-of-the-nutritional-value-of-meals-consumed-by-patients-with-recognized-schizophrenia
#18
Ewa Stefańska, Agnieszka Wendołowicz, Magdalena Lech, Karolina Wilczyńska, Beata Konarzewska, Joanna Zapolska, Lucyna Ostrowska
Background: As studies show, changes in diet - so important in the therapy of psychiatric disorders and related to changes in appetite and nutritional preferences, including avoiding of the consumption of specific groups of products and dishes - are much more frequent among patients affected by schizophrenia. Objective: The aim of the study was to assess the chosen nutritional habits, including the number and type of meals usually consumed during a day, snacking between meals and the energy value and content of the chosen nutrients in the diets of persons with recognized schizophrenia...
2018: Roczniki Państwowego Zakładu Higieny
https://www.readbyqxmd.com/read/29766694/analysis-of-the-choice-of-food-products-and-the-energy-value-of-diets-of-female-middle-and-long-distance-runners-depending-on-the-self-assessment-of-their-nutritional-habits
#19
Dominika Głąbska, Marta Jusińska
Background: Properly balanced diet is especially important in the case of young athletes, as it influences not only their physical development, but also influences results obtained during trainings and competitions. Objective: The aim of the study was to assess the choice of food products and the energy value of diets of female middleand long-distance runners, depending on the self-assessment of their nutritional habits. Material and methods: The study was conducted in the group of 40 female middle- and long-distance runners, aged 15-25, who declared average diet (n=15, 37...
2018: Roczniki Państwowego Zakładu Higieny
https://www.readbyqxmd.com/read/29766693/food-aversions-and-dietary-preferences-in-pre-school-children-from-olsztyn
#20
Joanna Ciborska, Jan Kłobukowski, Iwona Pierzchała
Background: The nutrition of pre-school children often does not follow the recommendations, and qualitatively and quantitatively limited selection of products in the diet shapes an attitude of aversion towards new products and dishes. The risk of deficiencies in many important nutrients emerges, particularly in vitamins and mineral components, which can affect the psychophysical development of the child and have an impact on nutritional status and health disorders in adulthood. Objective: Study of dietary preferences in pre-school children and identify possible food aversions...
2018: Roczniki Państwowego Zakładu Higieny
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