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https://www.readbyqxmd.com/read/28738724/neighbor-effect-penumbra-dose-exposed-neighbor-cells-contribute-to-the-enhanced-survival-of-high-dose-targeted-cells
#1
Min-Ho Lee, Ki-Man Lee, Eun-Hee Kim
PURPOSE: In the last decade, new types of "bystander effect" have been suggested by multiple research groups and have been challenged by others. In this study, we explored a new type of bystander effect, which has been defined in previous studies as the enhancement of the survival of high-dose targeted cells due to the penumbra-dose exposed neighbor cells. Intensity modulated radiation therapy, which is the most widely used treatment modality, generates local regions of gradient doses between targeted and shielded cells throughout the treatment volume; therefore, we were urged to ascertain whether the new type of effect is real and to suggest a revised treatment planning...
July 25, 2017: International Journal of Radiation Biology
https://www.readbyqxmd.com/read/28735851/assessing-beans-as-a-source-of-intrinsic-fiber-on-satiety-in-men-and-women-with-metabolic-syndrome
#2
Elizabeth J Reverri, Jody M Randolph, C Tissa Kappagoda, Eunyoung Park, Indika Edirisinghe, Britt M Burton-Freeman
Dietary fiber is well-known for its satiety inducing properties. Adding fibers to mixed dishes is one way to increase fiber intake. However, adding fibers to foods versus including foods inherently containing fiber may reveal differing effects on satiety. The present study aimed to explore the satiety effects of adding fiber to a mixed meal versus using beans (Phaseolus vulgaris) as a source of intrinsic fiber in the meal. In this pilot study, 12 men and women with metabolic syndrome were randomly assigned to eat three standard meals in a crossover design on three different occasions that contained either no added fiber (control (NF)), extrinsic or added fiber (AF), or whole black beans as the source of intrinsic fiber (BN)...
July 20, 2017: Appetite
https://www.readbyqxmd.com/read/28733393/microscopic-observation-of-living-cells-stained-with-fluorescent-probes
#3
Haruhiko Asakawa, Da-Qiao Ding, Tokuko Haraguchi, Yasushi Hiraoka
Fluorescence imaging of living cells provides a unique opportunity to follow dynamic behavior of specific molecules under physiological conditions. In the fission yeast Schizosaccharomyces pombe, expression of a target protein genetically fused with a fluorescent protein such as the jellyfish green fluorescent protein (GFP) is widely used. In addition, fluorescent chemical reagents are also used to stain specific molecules (e.g., Hoechst 33324 to stain DNA). Specimens of S. pombe cells for live cell imaging are prepared by either of two methods: sandwiching the cells between glass coverslips and by mounting the cells on a glass-bottom culture dish...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28732810/the-interaction-of-auraptene-and-other-oxyprenylated-phenylpropanoids-with-glucose-transporter-type-4
#4
Salvatore Genovese, Hitoshi Ashida, Yoko Yamashita, Tomoya Nakgano, Masaki Ikeda, Shirasaya Daishi, Francesco Epifano, Vito Alessandro Taddeo, Serena Fiorito
BACKGROUND: Glucose transporter 4 (GLUT4) is firmly established to play a pivotal role in glucose metabolism and in particular in modulating the insulin-stimulated glucose transport in several tissues, such as skeletal muscle and adipose tissue. Stimulation of GLUT4 by insulin results in its translocation to the plasma membrane, activation of several kinases, and finally in a large glucose influx into cells. PURPOSE: In this study we investigated the modulating properties of four biologically active oxyprenylated ferulic acid and umbelliferone derivatives and of their unprenylated parent compounds on GLUT-4 mediated glucose uptake and translocation...
August 15, 2017: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
https://www.readbyqxmd.com/read/28731641/macronutrient-content-and-food-exchanges-for-48-greek-mediterranean-dishes
#5
Paraskevi Detopoulou, Maria Aggeli, Elena Andrioti, Maria Detopoulou
AIM: The purpose of the present study was to facilitate the translation of traditional Greek Mediterranean recipes into food exchanges for diabetes. Moreover, it provides a useful food list for meal planning, which can be used by health professionals and nutritionists, as well as researchers and the public. METHODS: A total of 48 traditional Greek Mediterranean dishes were selected in order to include appetisers, sauces, salads, pies, dishes with vegetables and legumes as well as egg, pasta, rice, meat, fish and poultry-based dishes...
April 2017: Nutrition & Dietetics: the Journal of the Dietitians Association of Australia
https://www.readbyqxmd.com/read/28723268/assay-of-calcium-transients-and-synapses-in-rat-hippocampal-neurons-by-kinetic-image-cytometry-and-high-content-analysis-an-in-vitro-model-system-for-postchemotherapy-cognitive-impairment
#6
Patrick M McDonough, Natalie L Prigozhina, Ranor C B Basa, Jeffrey H Price
Postchemotherapy cognitive impairment (PCCI) is commonly exhibited by cancer patients treated with a variety of chemotherapeutic agents, including the endocrine disruptor tamoxifen (TAM). The etiology of PCCI is poorly understood. Our goal was to develop high-throughput assay methods to test the effects of chemicals on neuronal function applicable to PCCI. Rat hippocampal neurons (RHNs) were plated in 96- or 384-well dishes and exposed to test compounds (forskolin [FSK], 17β-estradiol [ES]), TAM or fulvestrant [FUL], aka ICI 182,780) for 6-14 days...
July 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28721669/mimicking-parkinson-s-disease-in-a-dish-merits-and-pitfalls-of-the-most-commonly-used-dopaminergic-in-vitro-models
#7
REVIEW
Fernanda Martins Lopes, Ivi Juliana Bristot, Leonardo Lisbôa da Motta, Richard B Parsons, Fabio Klamt
Parkinson's disease (PD) is the second most common neurodegenerative disorder and has both unknown etiology and non-curative therapeutic options. Patients begin to present the classic motor symptoms of PD-tremor at rest, bradykinesia and rigidity-once 50-70% of the dopaminergic neurons of the nigrostriatal pathway have degenerated. As a consequence of this, it is difficult to investigate the early-stage events of disease pathogenesis. In vitro experimental models are used extensively in PD research because they present a controlled environment that enables the direct investigation of the early molecular mechanisms that are potentially involved with dopaminergic degeneration, as well as for the screening of potential therapeutic drugs...
July 18, 2017: Neuromolecular Medicine
https://www.readbyqxmd.com/read/28721001/diffuse-idiopathic-skeletal-hyperostosis-dish-a-common-but-less-known-cause-of-back-pain
#8
REVIEW
Raju Vaishya, Vipul Vijay, Ifeanyi Charles Nwagbara, Amit K Agarwal
Diffuse idiopathic skeletal hyperostosis (DISH) is a systemic non-inflammatory disease of an unknown aetiology. It is characterized by ossification of the enthesis and may significantly affect the quality of life of the affected individuals. Despite the fact that this disease was first described more than 60 years ago and is a relatively common condition in the society, its awareness among clinician is still limited. An awareness of this entity and its mimicking conditions is essential so as to reach to an early diagnosis and avoid unnecessary investigations and treatment...
April 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28720939/cholesterol-analysis-of-korean-eat-out-foods-for-national-food-composition-database
#9
Farhana Sharmin, Beom-Gyun Jeong, Jiyoung Jung, Venus Quines, Jiyeon Chun
Information on cholesterol intake through restaurant meal is of high concern because of increasing eat-out population. Since nutrient labeling is not mandatory for restaurant food in Korea, cholesterol database on restaurant menu is unavailable. This study was performed to construct regional and national cholesterol database on primary Korean restaurant foods including 30 soup/stew, 24 rice dishes, and 27 noodles. From 2009 to 2012, Korea Ministry of Food and Drug Safety collected total 5832 foods (81 food types ×6 regions ×12 restaurants) nationwide and then 486 composites representing food types and regions were prepared for cholesterol analysis...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28720081/identification-of-an-easy-to-use-3d-culture-model-to-investigate-invasion-and-anticancer-drug-response-in-chondrosarcomas
#10
Eva Lhuissier, Céline Bazille, Juliette Aury-Landas, Nicolas Girard, Julien Pontin, Martine Boittin, Karim Boumediene, Catherine Baugé
BACKGROUND: Cytotoxic efficacy of anticancer drugs has been widely studied with monolayer-cultured cancer cells. However, the efficacy of drugs under two-dimensional (2D) culture condition usually differs from that of three-dimensional (3D) one. In the present study, an in vitro tumor tissue model was constructed using alginate hydrogel, and in vitro cytotoxic efficacy of two anticancer drugs (cisplatin and DZNep) was investigated in chondrosarcomas, and compared to in vivo response. METHODS: Three cell lines derived from human chondrosarcomas, CH2879, JJ012 and SW1353, were embedded in alginate hydrogel...
July 18, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28715366/continuous-wave-thulium-laser-for-heating-cultured-cells-to-investigate-cellular-thermal-effects
#11
Yoko Miura, Joachim Pruessner, Carla Lotta Mertineit, Katharina Kern, Michael Muenter, Moritz Moltmann, Veit Danicke, Ralf Brinkmann
An original method to heat cultured cells using a 1.94 µm continuous-wave thulium laser for biological assessment is introduced here. Thulium laser radiation is strongly absorbed by water, and the cells at the bottom of the culture dish are heated through thermal diffusion. A laser fiber with a diameter of 365 µm is set about 12 cm above the culture dish, without any optics, such that the laser beam diameter is almost equivalent to the inner diameter of the culture dish (30 mm). By keeping a consistent amount of culture medium in each experiment, it is possible to irradiate the cells with a highly reproducible temperature increase...
June 30, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28714585/mathematical-model-of-collective-cell-migrations-based-on-cell-polarity
#12
Masakazu Akiyama, Takamichi Sushida, Sumire Ishida, Hisashi Haga
Individual cells migrate toward the direction of the cell polarity generated by interior or exterior factors. Under situations without guides such as chemoattractants, they migrate randomly. On the other hand, it has been observed that cell groups lead to systematic collective cell migrations. For example, Dictyostelium discoideum and Madin-Darby canine kidney (epithelial) cells exhibit typical collective cell migration patterns such as uniformly directional migration and rotational migration. In particular, it has been suggested from experimental investigations that rotational migrations are intimately related to morphogenesis of organs and tissues in several species...
July 17, 2017: Development, Growth & Differentiation
https://www.readbyqxmd.com/read/28711418/intakes-and-sources-of-dietary-sugars-and-their-association-with-metabolic-and-inflammatory-markers
#13
Laura O'Connor, Fumiaki Imamura, Soren Brage, Simon J Griffin, Nicholas J Wareham, Nita G Forouhi
BACKGROUND & AIMS: Associations of dietary sugars with metabolic and inflammatory markers may vary according to the source of the sugars. The aim of this study was to examine the association of dietary sugars from different sources [beverages (liquids), foods (solids), extrinsic (free) or intrinsic (non-free)] with metabolic and inflammatory markers. METHODS: Population-based cross-sectional study of adults in the East of England (n = 9678). Sugar intakes were estimated using food frequency questionnaires...
June 17, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28706339/expansion-of-bone-marrow-derived-human-mesenchymal-stem-stromal-cells-hmscs-using-a-two-phase-liquid-liquid-system
#14
Mariana P Hanga, Halina Murasiewicz, Andrzej W Pacek, Alvin W Nienow, Karen Coopman, Christopher J Hewitt
BACKGROUND: Human mesenchymal stem/stromal cells (hMSCs) are at the forefront of regenerative medicine applications due to their relatively easy isolation and availability in adults, potential to differentiate and to secrete a range of trophic factors that could determine specialised tissue regeneration. To date, hMSCs have been successfully cultured in vitro on substrates such as polystyrene dishes (TCPS) or microcarriers. However, hMSC sub-cultivation and harvest typically employs proteolytic enzymes that act by cleaving important cell membrane proteins resulting in long-term cell damage...
July 2017: Journal of Chemical Technology and Biotechnology
https://www.readbyqxmd.com/read/28704823/different-behavior-and-response-of-staphylococcus-epidermidis-and-streptococcus-pneumoniae-to-a-ventriculoperitoneal-shunt-an-in-vitro-study
#15
Vahid Heidari, Zohreh Habibi, Ahmadreza Hojjati Marvasti, Zahra Ebrahim Soltani, Negin Naderian, Parvin Tanzifi, Farideh Nejat
BACKGROUND: Pneumococcal shunt infection is a rare event. There is no consensus on the therapeutic management of this kind of shunt infection according to literature reviews, and it seems to be different from infection with Staphylococcus epidermidis. We studied 2 shunted patients with pneumococcal meningitis, both of whom were treated with only antibiotics. The management of these cases seems to be different from that of shunt catheter infection due to these bacteria. We conducted a laboratory study to show the different behavior of pneumococcus compared to S...
July 14, 2017: Pediatric Neurosurgery
https://www.readbyqxmd.com/read/28704176/availability-and-promotion-of-healthful-foods-in-stores-and-restaurants-guam-2015
#16
Elizabeth A Lundeen, Brenna K VanFrank, Sandra L Jackson, Brittani Harmon, Alyssa Uncangco, Patrick Luces, Carrie Dooyema, Sohyun Park
Chronic disease, which is linked to unhealthy nutrition environments, is highly prevalent in Guam. The nutrition environment was assessed in 114 stores and 63 restaurants in Guam. Stores had limited availability of some healthier foods such as lean ground meat (7.5%) and 100% whole-wheat bread (11.4%), while fruits (81.0%) and vegetables (94.8%) were more commonly available; 43.7% of restaurants offered a healthy entrée or main dish salad, 4.1% provided calorie information, and 15.7% denoted healthier choices on menus...
July 13, 2017: Preventing Chronic Disease
https://www.readbyqxmd.com/read/28704145/adult-zebrafish-danio-rerio-an-alternative-behavioral-model-of-formalin-induced-nociception
#17
Francisco Ernani Alves Magalhães, Caio Átila Prata Bezerra de Sousa, Sacha Aubrey Alves Rodrigues Santos, Renata Barbosa Menezes, Francisco Lucas Alves Batista, Ângela Oliveira Abreu, Messias Vital de Oliveira, Luiz Francisco Wemmenson Gonçalves Moura, Ramon da Silva Raposo, Adriana Rolim Campos
The zebrafish (Danio rerio) has been proposed as a low-cost and simple alternative to the use of higher vertebrates in laboratory research on novel compounds with antinociceptive potential. In this study, we tested adult zebrafish (Danio rerio) as an alternative behavioral model of formalin-induced nociception. We evaluated the nociceptive effect of 0.1% formalin (3 or 5 μL; intramuscularly [i.m.]), applied into the tail or lips, on locomotor activity, using as parameter the number of times the fish crossed the lines between the quadrants of a glass Petri dish during the neurogenic stage (0-5 min) and the inflammatory stage (15-30 min)...
July 13, 2017: Zebrafish
https://www.readbyqxmd.com/read/28702885/use-of-a-super-hydrophobic-microbioreactor-to-generate-and-boost-pancreatic-mini-organoids
#18
Tiziana A L Brevini, Elena F M Manzoni, Sergio Ledda, Fulvio Gandolfi
Cell remarkable ability to self-organize and rearrange in functional organoids has been greatly boosted by the recent advances in 3-D culture technologies and materials. This approach can be presently applied to model human organ development and function "in a dish" and to predict drug response in a patient specific fashion.Here we describe a protocol that allows for the derivation of functional pancreatic mini-organoids from skin biopsies. Cells are suspended in a drop of medium and encapsulated with hydrophobic polytetrafluoroethylene (PTFE) powder particles, to form microbioreactors defined as "Liquid Marbles," that stimulate cell coalescence and 3-D aggregation...
July 13, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28699968/phytochemistry-antioxidant-potential-and-antifungal-of-byrsonima-crassifolia-on-soil-phytopathogen-control
#19
B S Andrade, R Matias, B O Corrêa, A K M Oliveira, D G F Guidolin, A R Roel
The use of chemical defensives to control fungal diseases has by consequence to impact negatively over the environment and human health, this way, the use of plant extracts with antifungal properties along with proper cultural management makes viable an alternative plant production control, specially for familiar and organic cultures. The objective of this study was to perform phytochemical and antioxidant analysis of Byrsonima crassifolia (canjiqueira) barks and evaluate its antifungal potential over Fusarium solani and Sclerotinia sclerotiorum mycelial growth...
July 10, 2017: Brazilian Journal of Biology, Revista Brasleira de Biologia
https://www.readbyqxmd.com/read/28693085/-molecular-characterization-genetic-diversity-of-extended-spectrum-%C3%AE-lactamases-harboring-conjugative-plasmids-identified-in-multi-drug-resistant-escherichia-coli-isolated-from-food-in-china
#20
L Bai, Z Pan, J Xu, F Q Li
Objective: The purpose of this study was to investigate the molecular characteristics of ESBL-encoding conjugative plasmid identified in muti-drug resistant Escherichia coli isolated from food. Methods: 465 Escherichia coli isolates were collected from national foodborne disease surveillance net from 2013 to 2014 (salad, n=159; meat, n=102; processed meat, n=95; cakes/rice, n=46; cooked dish, n=63). ESBLs strain was detected by Mueller-Hinton agar plate, and then its drug resistance was tested by agar dilution method...
July 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
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