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https://www.readbyqxmd.com/read/28223974/determination-of-antibacterial-and-technological-properties-of-vaginal-lactobacilli-for-their-potential-application-in-dairy-products
#1
Lorenzo Siroli, Francesca Patrignani, Diana I Serrazanetti, Carola Parolin, Rogers A Ñahui Palomino, Beatrice Vitali, Rosalba Lanciotti
Functional foods could differently affect human health in relation to the gender. Recent studies have highlighted the anti-Candida and anti-Chlamydia activities of some Lactobacillus strains isolated from the vagina of healthy women. Considering these important beneficial activities on women's health, the preparation of functional food containing active vaginal lactobacilli can represent a great scientific challenge for the female gender. In this context, the aim of this work was to study some functional and technological properties of 17 vaginal strains belonging to the species Lactobacillus crispatus, Lactobacillus gasseri, and Lactobacillus vaginalis in the perspective to include them in dairy products...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28221972/control-points-to-reduce-movement-of-central-nervous-system-tissue-during-beef-slaughter
#2
J L Aalhus, R D Thacker, I L Larsen, J C Roberts, M A Price, M Juárez
Consumption of central nervous system tissue (CNST) from cattle with bovine spongiform encephalopathy (BSE) is thought to cause the human neurological disease, variant Creutzfeldt-Jacob disease. To identify points of cross-contamination of beef carcasses with CNST, 55 young beef cattle were slaughtered and processed through a federally inspected multispecies abattoir. The objectives of this study were to evaluate CNST spread following the placement of a plug in the penetration site of the skull after captive bolt stunning, to evaluate cross-contamination of carcasses before and after splitting, to compare the effects of hot water pasteurization (84°C for 10 s) versus cold water wash (10°C for 30 s) for reducing CNST on the carcass, and to examine other possible sources of cross-contamination in the abattoir...
February 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28221923/matrix-specific-method-validation-of-an-automated-most-probable-number-system-for-use-in-measuring-bacteriological-quality-of-grade-a-milk-products
#3
Samantha Lindemann, Matthew Kmet, Ravinder Reddy, Steffen Uhlig
The U.S. Food and Drug Administration (FDA) oversees a long-standing cooperative federal and state milk sanitation program that uses the grade "A" Pasteurized Milk Ordinance standards to maintain the safety of grade "A" milk sold in the United States. The Pasteurized Milk Ordinance requires that grade "A" milk samples be tested using validated total aerobic bacterial and coliform count methods. The objective of this project was to conduct an interlaboratory method validation study to compare performance of a film plate method with an automated most-probable-number method for total aerobic bacterial and coliform counts, using statistical approaches from international data standards...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28221874/comparing-thermal-process-validation-methods-for-salmonella-inactivation-on-almond-kernels
#4
Sanghyup Jeong, Bradley P Marks, Michael K James
Ongoing regulatory changes are increasing the need for reliable process validation methods for pathogen reduction processes involving low-moisture products; however, the reliability of various validation methods has not been evaluated. Therefore, the objective was to quantify accuracy and repeatability of four validation methods (two biologically based and two based on time-temperature models) for thermal pasteurization of almonds. Almond kernels were inoculated with Salmonella Enteritidis phage type 30 or Enterococcus faecium (NRRL B-2354) at ~10(8) CFU/g, equilibrated to 0...
January 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28220111/identity-abundance-and-reactivation-kinetics-of-thermophilic-fermentative-endospores-in-cold-marine-sediment-and-seawater
#5
Marta Volpi, Bente Aa Lomstein, Andreas Sichert, Hans Røy, Bo B Jørgensen, Kasper U Kjeldsen
Cold marine sediments harbor endospores of fermentative and sulfate-reducing, thermophilic bacteria. These dormant populations of endospores are believed to accumulate in the seabed via passive dispersal by ocean currents followed by sedimentation from the water column. However, the magnitude of this process is poorly understood because the endospores present in seawater were so far not identified, and only the abundance of thermophilic sulfate-reducing endospores in the seabed has been quantified. We investigated the distribution of thermophilic fermentative endospores (TFEs) in water column and sediment of Aarhus Bay, Denmark, to test the role of suspended dispersal and determine the rate of endospore deposition and the endospore abundance in the sediment...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28215695/inactivation-of-viruses-by-pasteurization-at-60%C3%A2-%C3%A2-c-for-10%C3%A2-h-with-and-without-40-glucose-as-stabilizer-during-a-new-manufacturing-process-of-%C3%AE-2-macroglobulin-from-cohn-fraction-iv
#6
Chaoji Huangfu, Yuyuan Ma, Junting Jia, Maomin Lv, Fengxuan Zhu, Xiaowei Ma, Xiong Zhao, Jingang Zhang
Pasteurization is regularly used to inactivate viruses for the safety of plasma derivatives. Influence of pasteurization at 60 °C for 10 h on α2-Macroglobulin activity and virus inactivation were studied. With 40% sugar as stabilizers more than 70% α2-Macroglobulin activity was reserved after pasteurization compared with 20% in control. Glucose presented a better activity protection effect than sucrose and maltose. By pasteurization without stabilizer the virus titers of pseudorabies virus, Sindbis virus, porcine parvovirus and encephalomyocarditis virus were reduced more than 5...
February 16, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28214171/g-protein-based-elisa-as-a-potency-test-for-rabies-vaccines
#7
Martine Chabaud-Riou, Nadège Moreno, Fabien Guinchard, Marie Claire Nicolai, Elisabeth Niogret-Siohan, Nicolas Sève, Catherine Manin, Françoise Guinet-Morlot, Patrice Riou
The NIH test is currently used to assess the potency of rabies vaccine, a key criterion for vaccine release. This test is based on mice immunization followed by intracerebral viral challenge. As part of global efforts to reduce animal experimentation and in the framework of the development of Sanofi Pasteur next generation, highly-purified vaccine, produced without any material of human or animal origin, we developed an ELISA as an alternative to the NIH test. This ELISA is based on monoclonal antibodies recognizing specifically the native form of the viral G-protein, the major antigen that induces neutralizing antibody response to rabies virus...
February 14, 2017: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/28213026/heat-resistance-of-psychrotolerant-bacillus-cereus-vegetative-cells
#8
Alizée Guérin, Claire Dargaignaratz, Thierry Clavel, Véronique Broussolle, Christophe Nguyen-The
Spores of psychrotolerant strains of the foodborne pathogen Bacillus cereus can multiply during storage of cooked or pasteurized, refrigerated foods and can represent a risk if these cells are not eliminated during reheating of food product before consumption. We determined the heat-resistance of psychrotolerant B. cereus vegetative cells at different heating temperatures in laboratory medium and compared it with that of thermotolerant B. cereus vegetative cells. The z values, based on times for a 3 log10 reduction, of the vegetative cells of the three psychrotolerant phylogenetic groups of B...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28213025/influence-of-controlled-atmosphere-on-thermal-inactivation-of-escherichia-coli-atcc-25922-in-almond-powder
#9
Teng Cheng, Rui Li, Xiaoxi Kou, Shaojin Wang
Heat controlled atmosphere (CA) treatments hold potential to pasteurize Salmonella enteritidis PT 30 in almonds. Nonpathogenic Escherichia coli ATCC 25922 was used as a surrogate species of pathogenic Salmonella for validation of thermal pasteurization to meet critical safety requirements. A controlled atmosphere/heating block system (CA-HBS) was used to rapidly determine thermal inactivation of E. coli ATCC 25922. D- and z-values of E. coli ATCC 25922 inoculated in almond powder were determined at four temperatures between 65 °C and 80 °C under different gas concentrations and heating rates...
June 2017: Food Microbiology
https://www.readbyqxmd.com/read/28212540/expression-of-met-in-circulating-tumor-cells-correlates-with-expression-in-tumor-tissue-from-advanced-stage-lung-cancer-patients
#10
Marius Ilie, Edith Szafer-Glusman, Véronique Hofman, Elodie Long-Mira, Rebecca Suttmann, Walter Darbonne, Catherine Butori, Salomé Lalvée, Julien Fayada, Eric Selva, Wei Yu, Charles-Hugo Marquette, David S Shames, Elizabeth Punnoose, Paul Hofman
Given the difficulty in obtaining adequate tissue in NSCLC, we investigated the utility of circulating tumor cells (CTCs) for MET status assessment in NSCLC patients. We used two platforms for CTC capture, and assessed MET expression in CTCs and matched-bronchial biopsies in patients with advanced-stage III/IV lung adenocarcinoma. Baseline peripheral blood was collected from 256 advanced-stage III/IV NSCLC patients from Genentech clinical trials, and from 106 patients with advanced-stage III/IV lung adenocarcinoma treated at the Department of Pneumology, Pasteur Hospital, Nice...
February 15, 2017: Oncotarget
https://www.readbyqxmd.com/read/28210462/accuracy-of-a-new-rapid-antigen-detection-test-for-pulmonary-tuberculosis
#11
Rasoul Aliannejad, Ahmadreza Bahrmand, Hamidreza Abtahi, Mahnaz Seifi, Enayat Safavi, Farid Abdolrahimi, Shahriyar Shahriaran
BACKGROUND AND OBJECTIVES: Tuberculosis (TB) is a major problem in the world. Treatment and control of TB needs detection of the Mycobacterium tuberculosis (MT) in the proper samples. While smear doesn't have enough sensitivity, culture and PCR are expensive, time consuming and unavailable in many centers. Recent development of a rapid TB antigen detection test (PrTBK) at Pasteur Institute of Iran could give a simple way for diagnosis of TB in about two hours. In this test the antigen-antibody complex will change color when gold conjugated mouse anti-rabbit antibody detects specific MT cell wall antigen in suspected samples...
August 2016: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/28210223/consumer-willingness-to-pay-for-dengue-vaccine-cyd-tdv-dengvaxia-%C3%A2-in-brazil-implications-for-future-pricing-considerations
#12
Isabella P Godói, André S Santos, Edna A Reis, Livia L P Lemos, Cristina M R Brandão, Juliana Alvares, Francisco A Acurcio, Brian Godman, Augusto A Guerra Júnior
Introduction and Objective: Dengue virus is a serious global health problem with an estimated 3.97 billion people at risk for infection worldwide. In December 2015, the first vaccine (CYD-TDV) for dengue prevention was approved in Brazil, developed by Sanofi Pasteur. However, given that the vaccine will potentially be paid via the public health system, information is need regarding consumers' willingness to pay for the dengue vaccine in the country as well as discussions related to the possible inclusion of this vaccine into the public health system...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28208877/evaluation-of-antibiotic-residues-in-pasteurized-and-raw-milk-distributed-in-the-south-of-khorasan-e-razavi-province-iran
#13
Mortez Mohammadzadeh Moghadam, Mostafa Amiri, Hamed Ramezani Awal Riabi, Hamid Ramezani Awal Riabi
INTRODUCTION: The presence of antibiotic residues in milk and other products livestock is a health problem which can endanger public health. Antibiotics are used widely in animal husbandry to treat diseases related to bacterial infections. Antimicrobial drugs have been in use for decades in industry. They are commonly used in livestock facilities to treat mastitis. AIM: This study aimed to investigate antibiotic residues in pasteurized milk distributed in schools, in milk collection centers, and in milk production factories in Gonabad city...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28208853/genotypic-characterization-of-virulence-factors-in-escherichia-coli-isolated-from-patients-with-acute-cystitis-pyelonephritis-and-asymptomatic-bacteriuria
#14
Mohsen Tabasi, Mohammad Reza Asadi Karam, Mehri Habibi, Ehsan Mostafavi, Saeid Bouzari
INTRODUCTION: Urinary Tract Infections (UTIs) caused by Uropathogenic Escherichia coli (UPEC) are among the most common infections worldwide. It is well-documented that the pathogenesis of UPEC is mediated by the production of a wide variety of Virulence Factors (VFs). Thus, detection of these VFs and evaluation of their association with different clinical types of UTIs could help to understand the role of these factors in pathogenesis of UPEC isolates. AIM: To investigate the genotypic characteristics of UPEC isolates and to examine the relationship between VFs and different clinical symptoms of UTI...
December 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28197147/spoilage-of-microfiltered-and-pasteurized-extended-shelf-life-milk-is-mainly-induced-by-psychrotolerant-spore-forming-bacteria-that-often-originate-from-recontamination
#15
Etienne V Doll, Siegfried Scherer, Mareike Wenning
Premature spoilage and varying product quality due to microbial contamination still constitute major problems in the production of microfiltered and pasteurized extended shelf life (ESL) milk. Spoilage-associated bacteria may enter the product either as part of the raw milk microbiota or as recontaminants in the dairy plant. To identify spoilage-inducing bacteria and their routes of entry, we analyzed end products for their predominant microbiota as well as the prevalence and biodiversity of psychrotolerant spores in bulk tank milk...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28196121/prognostic-significance-of-chemotherapy-induced-necrosis-in-osteosarcoma-patients-receiving-pasteurized-autografts
#16
Min Wook Joo, Yong Koo Kang, Chang-Young Yoo, Sung Ho Cha, Yang-Guk Chung
BACKGROUND: Among various reconstruction methods after wide excision for osteosarcoma, pasteurized autograft is often preferred. While the whole area of the tumor can be assessed for chemotherapy-induced necrosis, one of the important prognostic factors, in other reconstructive techniques, only a portion removed from a wide-resection specimen is available when using pasteurized autograft method. The assessment, therefore, may be unreliable. We analyzed the prognostic significance of the chemotherapy-induced necrosis in osteosarcoma patients who underwent reconstruction with pasteurized autografts...
2017: PloS One
https://www.readbyqxmd.com/read/28195339/effect-of-high-voltage-atmospheric-cold-plasma-on-white-grape-juice-quality
#17
S K Pankaj, Zifan Wan, William Colonna, Kevin M Keener
BACKGROUND: This study focusses on the effects of novel, non-thermal high voltage atmospheric cold plasma (HVACP) processing on the quality of grape juice. A quality based comparison of cold plasma treatment of white grape juice was done with thermal pasteurization treatment. RESULTS: HVACP treatment of grape juice at 80 kV for 4 min resulted in 7.4 log10 cfu/mL reduction of S. cerevisiae without any significant (p > 0.05) change in pH, acidity and electrical conductivity of the juice...
February 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28195002/coverage-of-tendon-exposure-after-radial-forearm-free-flap-by-the-dorsoulnar-artery-perforator-flap
#18
Rémi Foissac, Marc Benatar, Olivier Dassonville, Alexandre Bozec, Gilles Poissonnet, Olivier Camuzard
Objective This study was designed to assess the effectiveness of an alternative technique using a perforator flap to manage secondary tendon exposure after a radial forearm free flap in head and neck oncologic surgery. Study Design Prospective cohort study. Setting Plastic Reconstructive Surgery Unit, Nice University Hospital, Pasteur 2 Hospital, France. Subjects and Methods Despite its numerous advantages, the radial forearm free flap is associated with significant donor site morbidity and the risk of secondary tendon exposure...
February 1, 2017: Otolaryngology—Head and Neck Surgery
https://www.readbyqxmd.com/read/28191640/pathogen-safety-of-a-pasteurized-four-factor-human-prothrombin-complex-concentrate-preparation-using-serial-20n-virus-filtration
#19
Thomas Nowak, Birgit Popp, Albrecht Gröner, Wolfram Schäfer, Uwe Kalina, Karlheinz Enssle, Nathan J Roth
BACKGROUND: Beriplex P/N/Kcentra/Coaplex/Confidex is a four-factor human prothrombin complex concentrate (PCC). Here, we describe the pathogen safety profile and biochemical characteristics of an improved manufacturing process that further enhances the virus safety of Beriplex P/N. STUDY DESIGN AND METHODS: Samples of product intermediates were spiked with test viruses, and prions were evaluated under routine production and robustness conditions of the scale-down version of the commercial manufacturing process for their capacity to inactivate or remove pathogens...
February 12, 2017: Transfusion
https://www.readbyqxmd.com/read/28186149/dielectric-properties-of-almond-kernels-associated-with-radio-frequency-and-microwave-pasteurization
#20
Rui Li, Shuang Zhang, Xiaoxi Kou, Bo Ling, Shaojin Wang
To develop advanced pasteurization treatments based on radio frequency (RF) or microwave (MW) energy, dielectric properties of almond kernels were measured by using an open-ended coaxial-line probe and impedance analyzer at frequencies between 10 and 3000 MHz, moisture contents between 4.2% to 19.6% w.b. and temperatures between 20 and 90 °C. The results showed that both dielectric constant and loss factor of the almond kernels decreased sharply with increasing frequency over the RF range (10-300 MHz), but gradually over the measured MW range (300-3000 MHz)...
February 10, 2017: Scientific Reports
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