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https://www.readbyqxmd.com/read/28812075/evidence-for-coherent-mixing-of-excited-and-charge-transfer-states-in-the-major-plant-light-harvesting-antenna-lhcii
#1
Charusheela Ramanan, Marco Ferretti, Henny van Roon, Vladimir I Novoderezhkin, Rienk van Grondelle
LHCII, the major light harvesting antenna from plants, plays a dual role in photosynthesis. In low light it is a light-harvester, while in high light it is a quencher that protects the organism from photodamage. The switching mechanism between these two orthogonal conditions is mediated by protein dynamic disorder and photoprotective energy dissipation. The latter in particular is thought to occur in part via spectroscopically 'dark' states. We searched for such states in LHCII trimers from spinach, at both room temperature and at 77 K...
August 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28807548/application-of-a-simple-column-switching-ion-chromatography-technique-for-removal-of-matrix-interferences-and-sensitive-fluorescence-determination-of-acidic-compounds-pharmaceutical-drugs-in-complex-samples
#2
Nadeem Muhammad, Qamar Subhani, Fenglian Wang, Dandan Guo, Qiming Zhao, Shuchao Wu, Yan Zhu
This work illustrates the introduction of a simple, rugged and flexible column-switching ion chromatography (IC) technique for an automated on-line QuEChERS extracted samples extracts washing followed by sensitive fluorescence (FLD) determination of five acidic pharmaceutical drugs namely; clofibric acid (CLO), ibuprofen (IBU), aspirin (ASP), naproxen (NAP) and flurobrofen (FLU) in three complex samples (spinach, apple and hospital sewage sludge). An old anion exchange column IonPac(®) AS11-HC was utilized as a pre-treatment column for on-line washing of inorganic and organic interferences followed by isocratic separation of five acidic drugs with another anion exchange IonPac(®) AS12A analytical column by exploiting the column-switching technique...
July 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28805458/thermal-inactivation-of-listeria-monocytogenes-and-salmonella-during-water-and-steam-blanching-of-vegetables
#3
Erdogan Ceylan, Wendy McMahon, Donna M Garren
Thermal inactivation of Listeria monocytogenes and Salmonella was evaluated on peas, spinach, broccoli, potatoes, and carrots that were treated with hot water and steam. One gram-positive bacterium, L. monocytogenes, and one gram-negative bacterium, Salmonella, were selected as pertinent human pathogens for evaluation. Samples were inoculated with a composite of five strains each of L. monocytogenes and Salmonella to achieve approximately 10(8) to 10(9) CFU/g. Inoculated samples were treated with hot water at 85 and 87...
August 14, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28801049/a-four-state-parametric-model-for-the-kinetics-of-the-non-photochemical-quenching-in-photosystem-ii
#4
Joris J Snellenburg, Matthew P Johnson, Alexander V Ruban, Rienk van Grondelle, Ivo H M van Stokkum
The phenomenon of non-photochemical quenching (NPQ) was studied in spinach chloroplasts using pulse amplitude modulated (PAM) fluorometry. We present a new analysis method which describes the observed fluorescence quantum yield as the sum of the product of four different states of PsiI and their corresponding quantum yields. These four distinct states are PsiI in the quenched or unquenched state, and with its reaction center either open or closed depending upon the reduction of the QA site. With this method we can describe the dynamics of the NPQ induction and recovery as well as quantify the percentage of photoinactivated RC throughout the measurement...
August 8, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28798859/amelioration-of-abnormalities-associated-with-the-metabolic-syndrome-by-spinacia-oleracea-spinach-consumption-and-aerobic-exercise-in-rats
#5
Vandana Panda, Kinjal Mistry, S Sudhamani, Mukesh Nandave, Shreesh Kumar Ojha
The present study evaluates the protective effects of an antioxidant-rich extract of Spinacea oleracea (NAOE) in abnormalities associated with the metabolic syndrome (MetS) in rats. HPTLC of NAOE revealed the presence of 13 total antioxidants, 14 flavonoids, and 10 phenolic acids. Rats administered with fructose (20% w/v) in drinking water for 45 days to induce abnormalities of MetS received NAOE (200 and 400 mg/kg, po), the standard drug gemfibrozil (60 mg/kg, po), aerobic exercise (AE), and a combination of NAOE 400 mg/kg and AE (NAOEAE) daily for 45 days...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28798380/contribution-of-cropland-to-the-spread-of-shiga-toxin-phages-and-the-emergence-of-new-shiga-toxin-producing-strains
#6
Pablo Quirós, Maite Muniesa
A growing interest in healthy eating has lead to an increase in the consumption of vegetables, associated with a rising number of bacterial outbreaks related to fresh produce. This is the case of the outbreak in Germany, caused by a O104:H4 enteroaggregative E. coli strain lysogenic for a Stx phage. Temperate Stx phages released from their hosts occur as free particles in various environments. This study reports the occurrence of Stx phages in vegetables (lettuce, cucumber, and spinach) and cropland soil samples...
August 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28795566/nucleotide-dependence-of-subunit-rearrangements-in-short-form-rubisco-activase-from-spinach
#7
Dayna S Peterson-Forbrook, Matthew T Hilton, Laura Tichacek, J Nathan Henderson, Hoang Quoc Bui, Rebekka M Wachter
Higher plant Rubisco activase (Rca) plays a critical role in regulating the activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). In vitro, Rca is known to undergo dynamic assembly-disassembly processes, with several oligomer stoichiometries coexisting over a broad concentration range. Although the hexamer appears to be the active form, changes in quaternary structure could play a role in Rubisco regulation. Therefore, fluorescent labels were attached to the C-termini of spinach beta-Rca, and the rate of subunit mixing was monitored by measuring energy transfer as a function of nucleotide and divalent cation...
August 10, 2017: Biochemistry
https://www.readbyqxmd.com/read/28783785/effect-of-soil-amendments-on-cd-accumulation-by-spinach-from-a-cd-mineralized-soil
#8
Adrian L D Paul, Rufus L Chaney
Cadmium (Cd)-mineralized soils occur in many nations. When these soils are noncalcareous, crops and especially leafy vegetables such as lettuce ( L.) and spinach ( L.) may accumulate levels of Cd in their edible portions that exceed international standards. Vegetable crops grown on Lockwood loam from Monterey County, CA, absorb an excessive amount of Cd into their edible portions. Agronomic or genetic management alternatives are needed to allow the use of these otherwise highly productive soils for spinach...
July 2017: Journal of Environmental Quality
https://www.readbyqxmd.com/read/28778009/plant-mediated-restriction-of-salmonella-enterica-on-tomato-and-spinach-leaves-colonized-with-pseudomonas-plant-growth-promoting-rhizobacteria
#9
Chiun-Kang Hsu, Shirley A Micallef
Reducing Salmonella enterica association with plants during crop production could reduce risks of fresh produce-borne salmonellosis. Plant growth-promoting rhizobacteria (PGPR) colonizing plant roots are capable of promoting plant growth and boosting resistance to disease, but the effects of PGPR on human pathogen-plant associations are not known. Two root-colonizing Pseudomonas strains S2 and S4 were investigated in spinach, lettuce and tomato for their plant growth-promoting properties and their influence on leaf populations of S...
July 27, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28766036/evaluation-of-the-antimicrobial-activities-of-ultrasonicated-spinach-leaf-extracts-using-rapd-markers-and-electron-microscopy
#10
Ammar Altemimi, Naoufal Lakhssassi, Amer Abu-Ghazaleh, David A Lightfoot
Spinach (Spinacia oleracea L.) leaves represent an important dietary source of nutrients, antioxidants and antimicrobials. As such, spinach leaves play an important role in health and have been used in the treatment of human diseases since ancient times. Here, the aims were to optimize the extraction methods for recovering antimicrobial substances of spinach leaves, determine the minimum inhibitory concentrations (MICs) of the antimicrobial substances against Escherichia coli and Staphylococcus aureus and, finally, evaluate the effects of spinach leaves' antimicrobials on bacterial DNA using central composite face-centered methods...
August 1, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28764135/potential-of-miscanthus-biochar-to-improve-sandy-soil-health-in-situ-nickel-immobilization-in-soil-and-nutritional-quality-of-spinach
#11
Waqas-Ud-Din Khan, Pia Muhammad Adnan Ramzani, Shazia Anjum, Farhat Abbas, Muhammad Iqbal, Abdullah Yasar, Muhammad Zahid Ihsan, Muhammad Naveed Anwar, Mujtaba Baqar, Hafiz Muhammad Tauqeer, Zaheer Abbas Virk, Shahbaz Ali Khan
The complex interaction of biochar (BC) with soil health reflecting properties, the feedstock used to prepare BC and application rate of BC in sandy soil is still a question for the researchers. An incubation study was conducted where nine different sorts of BC, each prepared from the different feedstock, were applied at 2% rate to evaluate their relative suitability to improve sandy soil health. Results revealed that BC prepared from miscanthus (MIB) significantly increased soil medium and fine pores, available water content (AWC), electrical conductivity (EC), and cation exchange capacity (CEC) while decreased soil wide pores, pH, bulk density (BD) and particle density (PD) compared to the rest sorts of BC...
October 2017: Chemosphere
https://www.readbyqxmd.com/read/28764001/investigation-of-degradation-and-penetration-behaviors-of-dimethoate-on-and-in-spinach-leaves-using-in-situ-sers-and-lc-ms
#12
Ruyan Hou, Mengmeng Tong, Wanjun Gao, Lei Wang, Tianxi Yang, Lili He
To investigate the degradation and penetration behaviors of the organophosphate insecticide dimethoate applied on spinach leaves, in situ SERS and LC-MS methods were used to detect dimethoate residue on&in spinach leaves picked on different days after treatment (DAT). The SERS and LC-MS methods determined that the dimethoate degradation rate followed first-order kinetics, with a half-life of 3.56 or 4.13days, depending on the respective method. The correlation coefficient of quantification value of these two methods was 0...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28761775/real-time-monitoring-of-superoxide-anion-radical-generation-in-response-to-wounding-electrochemical-study
#13
Ankush Prasad, Aditya Kumar, Ryo Matsuoka, Akemi Takahashi, Ryo Fujii, Yamato Sugiura, Hiroyuki Kikuchi, Shigeo Aoyagi, Tatsuo Aikawa, Takeshi Kondo, Makoto Yuasa, Pavel Pospíšil, Shigenobu Kasai
BACKGROUND: The growth and development of plants is deleteriously affected by various biotic and abiotic stress factors. Wounding in plants is caused by exposure to environmental stress, mechanical stress, and via herbivory. Typically, oxidative burst in response to wounding is associated with the formation of reactive oxygen species, such as the superoxide anion radical (O2(•-)), hydrogen peroxide (H2O2) and singlet oxygen; however, few experimental studies have provided direct evidence of their detection in plants...
2017: PeerJ
https://www.readbyqxmd.com/read/28753512/a-new-tool-for-direct-non-invasive-evaluation-of-chlorophyll-a-content-from-diffuse-reflectance-measurements
#14
M Muñoz-Ortuño, P Serra-Mora, R Herráez-Hernández, J Verdú-Andrés, P Campíns-Falcó
Chlorophyll is a key biochemical component that is responsible for photosynthesis and is an indicator of plant health. The effect of stressors can be determined by measuring the amount of chlorophyll a, which is the most abundant chlorophyll, in vegetation in general. Nowadays, invasive methods and vegetation indices are used for establishing chlorophyll amount or an approximation to this value, respectively. This paper demonstrates that H-point curve isolation method (HPCIM) is useful for isolating the signal of chlorophyll a from non-invasive diffuse reflectance measurements of leaves...
July 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28749669/thermal-degradation-of-major-gomphrenin-pigments-in-the-fruit-juice-of-basella-alba-l-malabar-spinach
#15
Agnieszka Kumorkiewicz, Slawomir Wybraniec
Generation of decarboxylated and dehydrogenated gomphrenins during heating of Basella alba L. fruit juice which contains high levels of betacyanin pigments was monitored by LC-DAD-ESI-MS/MS. The presence of principal decarboxylation products, 2-, 17- and 2,17-decarboxy-gomphrenins, their diastereomers as well as minor levels of their dehydrogenated derivatives are reported. In addition, determination of molecular masses of decarboxylated gomphrenins by high-resolution mass spectrometry (LCMS-IT-TOF) was performed...
July 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28738142/metabolomics-reveals-cu-oh-2-nanopesticide-activated-antioxidative-pathways-and-decreased-beneficial-antioxidants-in-spinach-leaves
#16
Lijuan Zhao, Yuxiong Huang, Adeyemi S Adeleye, Arturo A Keller
While the use of nanopesticides in modern agriculture continues to increase, their effects on crop plants are still poorly understood. Here, 4-week-old spinach plants grown in an artificial medium were exposed via foliar spray to Cu(OH)2 nanopesticide (0.18 and 18 mg/plant) or Cu ions (0.15 and 15 mg/plant) for 7 days. A gas chromatography-time of flight-mass spectrometry metabolomics approach was applied to assess metabolic alterations induced by Cu(OH)2 nanopesticide in spinach leaves. Exposure to Cu(OH)2 nanopesticide and copper ions induced alterations in the metabolite profiles of spinach leaves...
July 24, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28737734/storage-of-fruits-and-vegetables-in-refrigerator-increases-their-phenolic-acids-but-decreases-the-total-phenolics-anthocyanins-and-vitamin-c-with-subsequent-loss-of-their-antioxidant-capacity
#17
Joseph H Y Galani, Jalpesh S Patel, Nilesh J Patel, Jayant G Talati
It is of paramount importance for consumers, scientists and industrialists to understand how low-temperature storage of food items affects their bioactive compounds and properties. This study evaluated the effects of cold storage on total phenolics (TP), phenolic acids profile (PA), total anthocyanins (TA), total ascorbic acid (Vit. C) and antioxidant activity (AA) of 19 fruits and vegetables, collected from local Indian markets and stored in refrigerator (4 °C) during 15 days. Content of TP was highest in dill and amaranth and decreased (up to 29...
July 24, 2017: Antioxidants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28733762/reconsidering-betaine-as-a-natural-anti-heat-stress-agent-in-poultry-industry-a-review
#18
REVIEW
Muhammad Saeed, Daryoush Babazadeh, Muhammad Naveed, Muhammad Asif Arain, Faiz Ul Hassan, Sun Chao
Betaine is found ubiquitously in plants, animals, microorganisms, and rich dietary sources including seafood, spinach, and wheat bran. The chief physiological role of betaine is to function as a methyl donor and an osmolyte. Betaine also acts as an osmolyte, to maintain the avian's cellular water and ion balance to improve the avian's capacity against heat stress via preventing dehydration and osmotic inactivation. It helps in maintaining the protective osmolytic activity, especially in heat-stressed birds...
July 21, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28731633/australian-consumer-awareness-of-health-benefits-associated-with-vegetable-consumption
#19
Reetica Rekhy, Aila Khan, Jocelyn Eason, Hazel Mactavish-West, Carolyn Lister, Robyn Mcconchie
AIM: The present study investigated the perceived health benefits of specific vegetable consumption to guide the use of nutrition and health claims on vegetable marketing collateral. METHODS: Free elicitation and consumer ranking data were collected through an online survey of 1000 adults from across Australia and analysed for the perceived importance of vegetables in the daily diet, number of serves consumed per day, knowledge about health-related benefits of specific vegetables and perceived health benefits of vegetable consumption...
April 2017: Nutrition & Dietetics: the Journal of the Dietitians Association of Australia
https://www.readbyqxmd.com/read/28731339/colloidal-bound-polyphosphates-and-organic-phosphates-are-bioavailable-a-nutrient-solution-study
#20
Jessica Bollyn, Joran Faes, Andreas Fritzsche, Erik Smolders
Colloidal forms of Fe(III) minerals can be stabilized in solution by coatings of organic or poly-phosphate (P), which reduce the zeta-potential. This opens up a route toward the development of nanoforms of P fertilizers. However, it is unclear if such P forms are bioavailable. To address this question, spinach (Spinacia oleracea) was grown in nutrient solutions, at equal total P, using three different forms of P (orthophosphate = Pi; hexametaphosphate = HMP; myo-inositol hexaphosphate = IHP), free or bound to goethite/ferrihydrite colloids...
August 16, 2017: Journal of Agricultural and Food Chemistry
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