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https://www.readbyqxmd.com/read/28548831/folate-biofortification-in-hydroponically-cultivated-spinach-by-the-addition-of-phenylalanine
#1
Sho Watanabe, Yuta Ohtani, Yohei Tatsukami, Wataru Aoki, Takashi Amemiya, Yasunori Sukekiyo, Seiichi Kubokawa, Mitsuyoshi Ueda
Folate is an important vitamin mainly ingested from vegetables, and folate deficiency causes various health problems. Recently, several studies demonstrated folate biofortification in plants or food crops by metabolic engineering through genetic modifications. However, genetically modified foods may have adverse effects, and their production and sales are under strict regulation. Here, we developed a new approach to achieve folate biofortification in spinach (Spinacia oleracea) without genetic modification...
May 26, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28544262/synthesis-theoretical-studies-and-effect-on-the-photosynthetic-electron-transport-of-trifluoromethyl-arylamides
#2
Róbson Ricardo Teixeira, Marcus Vinícius de Andrade Barros, Gustavo Costa Bressan, Raoni Pais Siqueira, Fabíola Suelen Dos Santos, Michele Bertazzini, Rudolf Kiralj, Márcia Miguel Castro Ferreira, Giuseppe Forlani
BACKGROUND: The photosynthetic apparatus is targeted by various herbicides, including several amides such as diuron and linuron. Considering the need for the discovery of new active ingredients to cope with weed resistance, the synthesis of a series of trifluoromethyl aryl amides is herein described whose inhibitory properties were assessed in vitro on the photosynthetic electron transport chain, and in vivo on the growth of a model cyanobacterial strain. Theoretical studies were also carried out...
May 25, 2017: Pest Management Science
https://www.readbyqxmd.com/read/28541664/prediction-of-collision-cross-section-values-for-small-molecules-application-to-pesticide-residue-analysis
#3
Lubertus Bijlsma, Richard Bade, Alberto Celma, Lauren Mullin, Gareth Cleland, Sara Stead, Felix Hernandez, Juan Vicente Sancho
The use of collision cross-section (CCS) values obtained by ion mobility high-resolution mass spectrometry has added a third dimension (alongside retention time and exact mass) to aid in the identification of compounds. However, its utility is limited by the number of experimental CCS values currently available. This work demonstrates the potential of artificial neural networks (ANNs) for the prediction of CCS values of pesticides. The predictor, based on eight software-chosen mo-lecular descriptors, was optimised using CCS values of 205 small molecules and validated using a set of 131 pesticides...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28537264/draft-genome-of-spinach-and-transcriptome-diversity-of-120-spinacia-accessions
#4
Chenxi Xu, Chen Jiao, Honghe Sun, Xiaofeng Cai, Xiaoli Wang, Chenhui Ge, Yi Zheng, Wenli Liu, Xuepeng Sun, Yimin Xu, Jie Deng, Zhonghua Zhang, Sanwen Huang, Shaojun Dai, Beiquan Mou, Quanxi Wang, Zhangjun Fei, Quanhua Wang
Spinach is an important leafy vegetable enriched with multiple necessary nutrients. Here we report the draft genome sequence of spinach (Spinacia oleracea, 2n=12), which contains 25,495 protein-coding genes. The spinach genome is highly repetitive with 74.4% of its content in the form of transposable elements. No recent whole genome duplication events are observed in spinach. Genome syntenic analysis between spinach and sugar beet suggests substantial inter- and intra-chromosome rearrangements during the Caryophyllales genome evolution...
May 24, 2017: Nature Communications
https://www.readbyqxmd.com/read/28537029/arsenic-accumulation-and-physiological-attributes-of-spinach-in-the-presence-of-amendments-an-implication-to-reduce-health-risk
#5
Muhammad Shahid, Marina Rafiq, Nabeel Khan Niazi, Camille Dumat, Saliha Shamshad, Sana Khalid, Irshad Bibi
The current study examined the effect of calcium (Ca) and ethylenediaminetetraacetic acid (EDTA) on arsenic (As) uptake and toxicity to spinach (Spinacia oleracea) as well as assessed the potential human health risks. Spinach seedlings were exposed to three levels of As (25, 125, and 250 μM) alone or together with three levels of EDTA (25, 125, and 250 μM) and Ca (1, 5, and 10 mM). The effect of EDTA and Ca was assessed in terms of As contents in roots and shoots, hydrogen peroxide production, chlorophyll contents, and lipid peroxidation...
May 24, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28530571/novel-insights-into-ascorbate-retention-and-degradation-during-the-washing-and-post-harvest-storage-of-spinach-and-other-salad-leaves
#6
Rebecca A Dewhirst, Graham J J Clarkson, Steve D Rothwell, Stephen C Fry
Post-harvest treatments of pre-packaged salad leaves potentially cause l-ascorbate loss, but the mechanisms of ascorbate degradation remain incompletely understood, especially in planta. We explored the extent and pathways of ascorbate loss in variously washed and stored salad leaves. Ascorbate was assayed by 2,6-dichlorophenolindophenol titration, and pathways were monitored by (14)C-radiolabelling followed by high-voltage electrophoresis. All leaves tested showed ascorbate loss during storage: lettuce showed the greatest percentage loss, wild rocket the least...
October 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28511294/genome-sequences-of-spinach-deltapartitivirus-1-spinach-amalgavirus-1-and-spinach-latent-virus-identified-in-spinach-transcriptome
#7
Dongbin Park, Yoonsoo Hahn
Complete genome sequences of three new plant RNA viruses, Spinach deltapartitivirus 1 (SpDPV1), Spinach amalgavirus 1 (SpAV1), and Spinach latent virus (SpLV), were identified from a spinach (Spinacia oleracea) transcriptome dataset. The RNA-dependent RNA polymerases (RdRps) of SpDPV1, SpAV1, and SpLV showed 72%, 53%, and 93% amino acid sequence identities with the homologous RdRp of the most closely related virus in each case, respectively, suggesting that SpDPV1 and SpAV1 were novel viruses. Sequence similarity and phylogenetic analyses revealed that SpDPV1 belonged to the genus Deltapartitivirus of the family Partitiviridae, SpAV1 to the genus Amalgavirus of the family Amalgaviridae, and SpLV to the genus Ilarvirus of the family Bromoviridae...
May 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28505119/laxative-effects-of-dietary-supplementation-with-sujiaonori-algal-biomaterial-in-japanese-adult-women-with-functional-constipation-a-case-study
#8
Nlandu Roger Ngatu, Mitsunori Ikeda, Hiroyuki Watanabe, Mamoru Tanaka, Masataka Inoue
Constipation is a gastrointestinal motility disorder that represents a major health problem in Japan. Approximately 26% of young Japanese adult women are reported to have this complaint. We report on the health effects of daily intake of Sujiaonori algal biomaterial (SBM) on constipation on 12 Japanese adult women. Data are from a four-week dietary intervention study on the health effects of daily Sujiaonori supplementation on cardiovascular, skin, and gastrointestinal health in which 32 adult Japanese volunteers (age range: 20-54 years) participated...
May 15, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/28499881/spectral-dependence-of-irreversible-light-induced-fluorescence-quenching-chlorophyll-forms-with-maximal-emission-at-700-702-and-705-710nm-as-spectroscopic-markers-of-conformational-changes-in-the-core-complex
#9
Sherzod Nematov, Anna Paola Casazza, William Remelli, Vakhobjon Khuvondikov, Stefano Santabarbara
The spectral dependence of the irreversible non-photochemical fluorescence quenching associated with photoinhibition in vitro has been comparatively investigated in thylakoid membranes, PSII enriched particles and PSII core complexes isolated from spinach. The analysis of the fluorescence emission spectra of dark-adapted and quenched samples as a function of the detection temperature in the 280-80K interval, indicates that Chlorophyll spectral forms having maximal emission in the 700-702nm and 705-710nm ranges gain relative intensity in concomitance with the establishment of irreversible light-induced quenching, acting thereby as spectroscopic markers...
May 9, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28497193/evaluation-of-new-cu-ii-complexes-as-a-novel-class-of-inhibitors-against-plant-carbonic-anhydrase-glutathione-reductase-and-photosynthetic-activity-in-photosystem-ii
#10
Margarita V Rodionova, Sergei K Zharmukhamedov, Mehmet Sayım Karacan, Kubra Begum Venedik, Alexandr V Shitov, Turgay Tunç, Serhat Mamaş, Vladimir D Kreslavski, Nurcan Karacan, Vyacheslav V Klimov, Suleyman I Allakhverdiev
Increasing inefficiency of production of important agricultural plants raises one of the biggest problems in the modern world. Herbicide application is still the best method of weed management. Traditional herbicides blocking only one of the plant metabolic pathways is ineffective due to the rapid growth of herbicide-resistant weeds. The synthesis of novel compounds effectively suppressing several metabolic processes, and therefore achieving the synergism effect would serve as the alternative approach to weed problem...
May 11, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28484233/mercury-pollution-in-vegetables-grains-and-soils-from-areas-surrounding-coal-fired-power-plants
#11
Rui Li, Han Wu, Jing Ding, Weimin Fu, Lijun Gan, Yi Li
Mercury contamination in food can pose serious health risks to consumers and coal-fired power plants have been identified as the major source of mercury emissions. To assess the current state of mercury pollution in food crops grown near coal-fired power plants, we measured the total mercury concentration in vegetables and grain crops collected from farms located near two coal-fired power plants. We found that 79% of vegetable samples and 67% of grain samples exceeded the PTWI's food safety standards. The mercury concentrations of soil samples were negatively correlated with distances from the studied coal-fired power plants, and the mercury contents in lettuce, amaranth, water spinach, cowpea and rice samples were correlated with the mercury contents in soil samples, respectively...
May 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28480414/nitrate-in-leafy-green-vegetables-and-estimated-intake
#12
Danijel Brkić, Jasna Bošnir, Martina Bevardi, Andrea Gross Bošković, Sanja Miloš, Dario Lasić, Adela Krivohlavek, Aleksandar Racz, Ana Mojsović Ćuić, Natalija Uršulin Trstenjak
BACKGROUND: Vegetarian diets are rich in vegetables. Green leafy vegetables are foods that contain considerable amounts of nitrate, which can have both positive and negative effects on the human body. Their potential carcinogenicity and toxicity have been proven, particularly after the reduction of nitrate to nitrite itself or just serving as a reactant with amines and/or amides in the formation of N-nitroso compounds -N-nitrosamines and other nitrogen compounds which may have high levels of nitrate...
2017: African Journal of Traditional, Complementary, and Alternative Medicines: AJTCAM
https://www.readbyqxmd.com/read/28463566/influence-of-groundwater-and-wastewater-irrigation-on-lead-accumulation-in-soil-and-vegetables-implications-for-health-risk-assessment-and-phytoremediation
#13
Sana Khalid, Muhammad Shahid, Camille Dumat, Nabeel Khan Niazi, Irshad Bibi, Hafiz Faiq Sidique Gul Bakhat, Ghulam Abbas, Behzad Murtaza, Hafiz Muhammad Rashid Javeed
The current study evaluated the effect of groundwater and wastewater irrigation on lead (Pb) accumulation in soil and vegetables, and its associated health implications. A pot experiment was conducted in which spinach (Spinacia oleracea), radish (Raphanus sativus) and cauliflower (Brassica oleracea) were irrigated with groundwater and wastewaters containing varying concentrations of Pb. Lead contents were measured in wastewaters, soils and root and shoot of vegetables. We also measured health risk index (HRI) associated with the use of vegetables irrigated by wastewaters...
May 2, 2017: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/28462718/rubiscolins-highly-potent-peptides-derived-from-plant-proteins
#14
Renata Perlikowska, Anna Janecka
Rubiscolins are two naturally occurring, linear peptides, isolated from the pepsin digests of D-ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) from spinach leaves. Their sequences are as follows: Tyr-Pro-Leu-Asp-Leu (YPLDL, rubiscolin-5) and Tyr-Pro-Leu-Asp-Leu-Phe (YPLDLF, rubiscolin-6, also known as rubixyl). Though their structure does not resemble typical opioid peptides, rubiscolins were found to exhibit high affinity and selectivity for the delta opioid receptor and an antinociceptive effect in mice after oral administration...
April 26, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28448865/glycolipids-from-spinach-suppress-lps-induced-vascular-inflammation-through-enos-and-nk-%C3%AE%C2%BAb-signaling
#15
Masakazu Ishii, Tatsuo Nakahara, Daisuke Araho, Juri Murakami, Masahiro Nishimura
Glycolipids are the major constituent of the thylakoid membrane of higher plants and have a variety of biological and pharmacological activities. However, anti-inflammatory effects of glycolipids on vascular endothelial cells have not been elucidated. Here, we investigated the effect of glycolipids extracted from spinach on lipopolysaccharides (LPS)-induced endothelial inflammation and evaluated the underlying molecular mechanisms. Treatment with glycolipids from spinach had no cytotoxic effects on cultured human umbilical vein endothelial cells (HUVECs) and significantly blocked the expression of LPS-induced interleukin (IL)-6, monocyte chemoattractant protein-1 (MCP-1), vascular cell adhesion molecule-1 (VCAM-1), and intracellular adhesion molecule-1 (ICAM-1) in them...
April 24, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28437617/electrostatic-origin-of-the-red-solvatochromic-shift-of-dfhbdi-in-rna-spinach
#16
Samik Bose, Suman Chakrabarty, Debashree Ghosh
Interactions with the environment tune the spectral properties of biological chromophores, e.g., fluorescent proteins. Understanding the relative contribution of the various types of noncovalent interactions in the spectral shifts can provide rational design principles toward developing new fluorescent probes. In this work, we investigate the origin of the red shift in the absorption spectra of the difluoro hydroxybenzylidene dimethyl imidazolinone (DFHBDI) chromophore in RNA spinach as compared to the aqueous solution...
May 11, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28432538/superoxide-generated-in-the-chloroplast-stroma-causes-photoinhibition-of-photosystem-i-in-the-shade-establishing-tree-species-psychotria-henryi
#17
Wei Huang, Ying-Jie Yang, Jiao-Lin Zhang, Hong Hu, Shi-Bao Zhang
Our previous studies indicated that high light induced significant photoinhibition of photosystem I (PSI) in the shade-establishing tree species Psychotria henryi. However, the underlying mechanism has not been fully clarified. In the present study, in order to investigate the mechanism of PSI photoinhibition in P. henryi, we treated detached leaves with constant high light in the presence of methyl viologen (MV) or a soluble α-tocopherol analog, 2,2,5,7,8-pentamethyl-6-chromanol (PMC). We found that MV significantly depressed photochemical quantum yields in PSI and PSII when compared to PMC...
April 21, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28428863/switching-among-natal-and-auxiliary-hosts-increases-vulnerability-of-spodoptera-exigua-h%C3%A3-bner-lepidoptera-noctuidae-to-insecticides
#18
Qamar Saeed, Shafqat Saeed, Faheem Ahmad
The role of insecticidal application and host plant resistance in managing Spodoptera exigua has been well documented, but the effect of different host plants, on which the pest cycles its population in the field, has seldom been investigated. Therefore, we have studied the vulnerability of S. exigua against commonly used insecticides (cypermethrin, chlorpyrifos, lufenuron, and emamectin benzoate) with different mode of actions when it switches its generations from natal to auxiliary hosts and vice versa. Different field populations being established on different host plants including castor, cauliflower, cotton, okra, and spinach were collected and reared in the laboratory before insecticidal bioassays...
April 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28425510/a-universal-split-spinach-aptamer-ussa-for-nucleic-acid-analysis-and-dna-computation
#19
Nanami Kikuchi, Dmitry M Kolpashchikov
We demonstrate how a single universal spinach aptamer (USSA) probe can be used to detect multiple (potentially any) nucleic acid sequences. USSA can be used for cost-efficient and highly selective analysis of even folded DNA and RNA analytes, as well as for the readout of outputs of DNA logic circuits.
April 20, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28413190/virgin-olive-oil-enriched-with-lutein-zeaxanthin-from-spinacia-oleracea
#20
María Benito de Valle-Prieto, Jonathan Delgado-Adámez, Ma Victoria Gil, Sara Martillanes, Ma Nieves Franco, Daniel Martín-Vertedor
The aim of this work consists of developing a technological process for elaborating a virgin olive oil enriched in lutein-zeaxanthin extracted from spinach, studying different parameters like temperature, time of extraction and different ratios (spinach-oil). It was observed that the amount of carotenoids extracted increased up to a maximum after 24 hours and decreased as the maceration time progressed up to 60 hours, resulting of biological degradation. It was also observed that as more spinach we added, as more lutein-zeaxanthin in the enriched virgin olive oil was obtained...
April 13, 2017: Journal of Oleo Science
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