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https://www.readbyqxmd.com/read/28345909/rapid-and-simultaneous-analysis-of-360-pesticides-in-brown-rice-spinach-orange-and-potato-using-microbore-gc-ms-ms
#1
Jonghwa Lee, Leesun Kim, Yongho Shin, Junghak Lee, Jiho Lee, Eunhye Kim, Joon-Kwan Moon, Jeong-Han Kim
A multi-residue method for simultaneous and rapid analysis of 360 pesticides in representative agricultural produce (brown rice, orange, spinach, and potato) was developed using a modified QuEChERS procedure combined with gas chromatography tandem mass spectrometry (GC-MS/MS). Selected reaction monitoring transition parameters (e.g., collision energy, precursor and product ions) in MS/MS were optimized to achieve the best selectivity and sensitivity for a wide range of GC amenable pesticides. A short (20 m) microbore (0...
March 27, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28341915/in-vivo-system-for-analyzing-the-function-of-the-psbp-protein-using-chlamydomonas-reinhardtii
#2
Taishi Nishimura, Fumihiko Sato, Kentaro Ifuku
The PsbP protein is an extrinsic subunit of photosystem II (PSII) specifically developed in green-plant species including land plants and green algae. The protein-protein interactions involving PsbP and its effect on oxygen evolution have been investigated in vitro using isolated PSII membranes. However, the importance of those interactions needs to be examined at the cellular level. To this end, we developed a system expressing exogenous PsbP in the background of the Chlamydomonas BF25 mutant lacking native PsbP...
March 24, 2017: Photosynthesis Research
https://www.readbyqxmd.com/read/28334800/optimization-of-a-novel-biophysical-model-using-large-scale-in-vivo-antisense-hybridization-data-displays-improved-prediction-capabilities-of-structurally-accessible-rna-regions
#3
Jorge Vazquez-Anderson, Mia K Mihailovic, Kevin C Baldridge, Kristofer G Reyes, Katie Haning, Seung Hee Cho, Paul Amador, Warren B Powell, Lydia M Contreras
Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a thermodynamic understanding of RNA-RNA interactions. However, these approaches depend on structure predictions and have limited accuracy, arguably due to overlooking important cellular environment factors. In this work, we develop a biophysical model to describe asRNA-RNA hybridization that incorporates in vivo factors using large-scale experimental hybridization data for three model RNAs: a group I intron, CsrB and a tRNA. A unique element of our model is the estimation of the availability of the target region to interact with a given asRNA using a differential entropic consideration of suboptimal structures...
February 21, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28319798/cold-plasma-activated-hydrogen-peroxide-aerosol-inactivates-escherichia-coli-o157-h7-salmonella-typhimurium-and-listeria-innocua-and-maintains-quality-of-grape-tomato-spinach-and-cantaloupe
#4
Yunbin Jiang, Kimberly Sokorai, Georgios Pyrgiotakis, Philip Demokritou, Xihong Li, Sudarsan Mukhopadhyay, Tony Jin, Xuetong Fan
The purpose of this study was to investigate the efficacy of aerosolized hydrogen peroxide in inactivating bacteria and maintaining quality of grape tomatoes, baby spinach leaves and cantaloupes. Stem scars and smooth surfaces of tomatoes, spinach leaves, and cantaloupe rinds, inoculated with Escherichia coli O157:H7, Salmonella Typhimurium and Listeria innocua, were treated for 45s followed by additional 30min dwell time with hydrogen peroxide (7.8%) aerosols activated by atmospheric cold plasma. Non-inoculated samples were used to study the effects on quality and native microflora populations...
March 10, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28316477/effect-of-fermented-spinach-as-sources-of-pre-converted-nitrite-on-color-development-of-cured-pork-loin
#5
Tae-Kyung Kim, Young-Boong Kim, Ki-Hong Jeon, Jong-Dae Park, Jung-Min Sung, Hyun-Wook Choi, Ko-Eun Hwang, Yun-Sang Choi
The effect of fermented spinach extracts on color development in cured meats was investigated in this study. The pH values of raw cured meats without addition of fermented spinach extract or nitrite (negative control) were higher (p<0.05) than those added with fermented spinach extract. The pH values of raw and cooked cured meats in treatment groups were decreased with increasing addition levels of fermented spinach extract. The lightness and yellowness values of raw cured meats formulated with fermented spinach extract were higher (p<0...
2017: Korean Journal for Food Science of Animal Resources
https://www.readbyqxmd.com/read/28281009/verrucarin-a-and-roridin-e-produced-on-spinach-by-myrothecium-verrucaria-under-different-temperatures-and-co2-levels
#6
Ilenia Siciliano, Pietro Bosio, Giovanna Gilardi, Maria Lodovica Gullino, Angelo Garibaldi
The behavior of Myrothecium verrucaria, artificially inoculated on spinach, was studied under seven different temperature conditions (from 5 to 35 °C) and under eight different combinations of temperature and CO2 concentration (14-30 °C and 775-870 or 1550-1650 mg/m(3)). The isolate used for this study was growing well on spinach, and the mycotoxins verrucarin A and roridin E were produced under all tested temperature and CO2 conditions. The maximum levels of verrucarin A (18.59 ng/g) and roridin E (49...
March 9, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28280510/determination-of-levels-of-organochlorine-organophosphorus-and-pyrethroid-pesticide-residues-in-vegetables-from-markets-in-dar-es-salaam-by-gc-ms
#7
John A M Mahugija, Farhat A Khamis, Esther H J Lugwisha
This study investigated the levels of pesticides and metabolites in vegetables from major markets in Dar es Salaam city, Tanzania. Samples of fresh cabbage, spinach, and onions from the markets were analysed for pesticide residues. Extraction was performed using acetone followed by dichloromethane : cyclohexane mixture and the extracts were cleaned up using Florisil. The compounds were determined by gas chromatography-mass spectrometry (GC-MS). Pesticides and metabolites were detected in 72.2% of the samples...
2017: International Journal of Analytical Chemistry
https://www.readbyqxmd.com/read/28276907/dietary-sources-of-polyphenols-in-the-mediterranean-healthy-eating-aging-and-lifestyle-meal-study-cohort
#8
Justyna Godos, Stefano Marventano, Antonio Mistretta, Fabio Galvano, Giuseppe Grosso
The aim of this study was to estimate the dietary intake and major food sources of polyphenols in the Mediterranean healthy Eating, Aging and Lifestyles (MEAL) study cohort. A total of 1937 individuals (18 + y) of urban population of Catania, Italy, completed a validated 110-item food frequency questionnaire; Phenol-Explorer database was used to estimate polyphenol intake. Mean intake of polyphenols was 663.7 mg/d; the most abundant classes were phenolic acids (362.7 mg/d) and flavonoids (258.7 mg/d)...
February 9, 2017: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/28276484/acetylation-and-phosphorylation-control-both-local-and-global-stability-of-the-chloroplast-f1-atp-synthase
#9
Carla Schmidt, Victoria Beilsten-Edmands, Shabaz Mohammed, Carol V Robinson
ATP synthases (ATPases) are enzymes that produce ATP and control the pH in the cell or cellular compartments. While highly conserved over different species, ATPases are structurally well-characterised but the existence and functional significance of many post-translational modifications (PTMs) is not well understood. We combined a range of mass spectrometric techniques to unravel the location and extent of PTMs in the chloroplast ATP synthase (cATPase) purified from spinach leaves. We identified multiple phosphorylation and acetylation sites and found that both modifications stabilise binding of ε and δ subunits...
March 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28272919/in-situ-generation-of-chlorine-dioxide-for-surface-decontamination-of-produce
#10
Cheng-An Hwang, Lihan Huang, Vivian Chi-Hua Wu
Fresh fruits and vegetables are frequently contaminated with bacterial pathogens and implicated in foodborne illnesses. The objective of this study was to develop a unique surface decontamination method for produce using sodium chlorite and an acid in a sequential treatment. The surfaces of cantaloupe rinds, peels of cucumbers, stem scars of grape tomatoes, and leaves of baby spinach were inoculated with Salmonella or Listeria monocytogenes at 5 to 6 log CFU/g, submerged in 1.6 to 4% sodium chlorite solutions for 10 or 30 min, dried for 20 min, and then soaked in 6 mM hydrogen chloride (HCl) for 10 or 30 min and dried for 20 min...
March 8, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28265366/micronutrient-composition-and-acceptability-of-moringa-oleifera-leaf-fortified-dishes-by-children-in-ada-east-district-ghana
#11
Mary Glover-Amengor, Richmond Aryeetey, Edwin Afari, Alexander Nyarko
Moringa oleifera (M. oleifera), a green leafy vegetable, is a good source of minerals and vitamins which could be consumed as part of diet to improve human health and well-being. Drying M. oleifera leaves could make it readily available for use as a food fortificant. The objectives of the study were to determine micromineral and β -carotene content of dried M. oleifera leaves, and leaf-incorporated local dishes, and also assess school children's (4-12 yr) acceptability of dried M. oleifera leaf-incorporated local dishes and feasibility of introducing dried leaves into a school lunch menu...
March 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28260733/survey-of-nitrate-ion-concentrations-in-vegetables-cultivated-in-plant-factories-comparison-with-open-culture-vegetables
#12
COMPARATIVE STUDY
Yuka Oka, Izumi Hirayama, Mitsuhide Yoshikawa, Tomoko Yokoyama, Kenji Iida, Katsushi Iwakoshi, Ayana Suzuki, Midori Yanagihara, Yukino Segawa, Sonomi Kukimoto, Humika Hamada, Satomi Matsuzawa, Setsuko Tabata, Takeo Sasamoto
A survey of nitrate-ion concentrations in plant-factory-cultured leafy vegetables was conducted. 344 samples of twenty-one varieties of raw leafy vegetables were examined using HPLC. The nitrate-ion concentrations in plant-factory-cultured leafy vegetables were found to be LOD-6,800 mg/kg. Furthermore, the average concentration values varied among different leafy vegetables. The average values for plant-factory-cultured leafy vegetables were higher than those of open-cultured leafy vegetables reported in previous studies, such as the values listed in the Standard Tables of Food Composition in Japan- 2015 - (Seventh revised edition)...
2017: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/28249191/inoculating-chlamydospores-of-trichoderma-asperellum-sm-12f1-changes-arsenic-availability-and-enzyme-activity-in-soils-and-improves-water-spinach-growth
#13
Shiming Su, Xibai Zeng, Lingyu Bai, Paul N Williams, Yanan Wang, Lili Zhang, Cuixia Wu
Arsenic (As)-contaminated agricultural soils threaten crop yields and pose a human health risk. Augmentation of exogenous microorganisms exhibiting plant-growth promoting and As speciation changing shows potential to improve crop growth and change soil As availability. Trichoderma asperellum SM-12F1 exhibiting both traits was developed into chlamydospores to improve its persistence in contaminated soils. After inoculation, As availability and enzyme activity in two types of soils and the growth as well as As uptake of water spinach (Ipomoea aquatic Forsk...
May 2017: Chemosphere
https://www.readbyqxmd.com/read/28248635/health-risk-assessment-of-dietary-cadmium-intake-do-current-guidelines-indicate-how-much-is-safe
#14
Soisungwan Satarug, David A Vesey, Glenda C Gobe
BACKGROUND: Cadmium (Cd), a food-chain contaminant, is a significant health hazard. The kidney is one of the primary sites of injury after chronic Cd exposure. Kidney-based risk assessment establishes the urinary Cd threshold at 5.24 μg/g creatinine, and tolerable dietary intake of Cd at 62 μg/day per 70-kg person. However, cohort studies show that dietary Cd intake below a threshold limit and that tolerable levels may increase the risk of death from cancer, cardiovascular disease, and Alzheimer's disease...
March 2017: Environmental Health Perspectives
https://www.readbyqxmd.com/read/28242932/dietary-fat-composition-food-matrix-and-relative-polarity-modulate-the-micellarization-and-intestinal-uptake-of-carotenoids-from-vegetables-and-fruits
#15
Purna Chandra Mashurabad, Ravindranadh Palika, Yvette Wilda Jyrwa, K Bhaskarachary, Raghu Pullakhandam
Dietary fat increases carotenoid bioavailability by facilitating their transfer to the aqueous micellar fraction during digestion. However, the specific effect of both quantity and type of dietary fat required for optimal carotenoid absorption remained unexplored. In the present study, the effect of amount and type of vegetable oils on carotenoid micellarization from carrot, spinach, drumstick leaves and papaya using in vitro digestion/Caco-2 cell model have been assessed. Although, dietary fat (0.5-10% w/w) significantly increased the micellarization of carotenoids from all the test foods, the extent of increase was determined by the food matrix (papaya > drumstick = spinach > carrot) and polarity of carotenoids (lutein > β-carotene = α-carotene > lycopene)...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28240917/regulated-electron-tunneling-of-photoinduced-primary-charge-separated-state-in-the-photosystem-ii-reaction-center
#16
Masashi Hasegawa, Hiroki Nagashima, Reina Minobe, Takashi Tachikawa, Hiroyuki Mino, Yasuhiro Kobori
In initial events of the photosynthesis by higher plants, the photosystem II (PSII) generates photoinduced primary charge-separated (CS) state composed of reduced pheophytin (PheoD1(-•)) and oxidized special pair (P(+•)) in chlorophyll a (Chla) PD1/PD2 in the D1/D2 heterodimer, ultimately leading to the water oxidation at the oxygen-evolving Mn4CaO5 cluster by P(+•). To understand the mechanism of the efficient generation of initially localized CS state (PD1(+•) PheoD1(-•)), we have characterized cofactor geometries and electronic coupling of the photoinduced primary CS state in quinone prereduced membrane of PSII from spinach using the time-resolved electron paramagnetic resonance method...
February 27, 2017: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/28238891/appetite-suppressing-effect-of-spinacia-oleracea-in-rats-involvement-of-the-short-term-satiety-signal-cholecystokinin
#17
Vandana Panda, Priyanka Shinde
Spinacia oleracea (spinach) is a green leafy vegetable rich in antioxidant phyto-constituents such as flavonoids, polyphenols, carotenoids and vitamins. Fruits and vegetables rich in flavonoids are known to prevent weight gain by inducing satiety. The present study evaluates the appetite suppressing effect of a flavonoid rich extract of the spinach leaf (SOE) in rats. HPTLC of SOE was performed for detecting flavonoids. Rats were administered SOE (200 mg/kg and 400 mg/kg, p. o) and fluoxetine (6 mg/kg i...
February 24, 2017: Appetite
https://www.readbyqxmd.com/read/28235460/tyrozine-d-oxidation-and-redox-equilibrium-in-photosystem-ii
#18
Nigar Ahmadova, Felix Ho, Stenbjorn Styring, Fikret Mamedov
Tyrosine D (TyrD) is an auxiliary redox active tyrosine residue in photosystem II (PsiI). The mechanism of TyrD oxidation was investigated by EPR spectroscopy, flash-induced fluorescence decay and thermoluminescence measurements in PsiI enriched membranes from spinach. PsiI membranes were chemically treated with 3mM ascorbate and 1mM diaminodurene and subsequent washing, leading to the complete reduction of TyrD. TyrD oxidation kinetics and competing recombination reactions were measured after a single saturating flash in the absence and presence of DCMU (inhibitor of the QB-site) in the pH range of 4...
February 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28231123/antibacterial-and-antioxidant-activities-of-essential-oils-from-artemisia-herba-alba-asso-pelargonium-capitatum-%C3%A3-radens-and-laurus-nobilis-l
#19
Ragina Rafiq, Saeed A Hayek, Ugochukwu Anyanwu, Bonita I Hardy, Valerie L Giddings, Salam A Ibrahim, Reza Tahergorabi, Hye Won Kang
Essential oils are natural antimicrobials that have the potential to provide a safer alternative to synthetic antimicrobials currently used in the food industry. Therefore, the aim of this study was to evaluate the antimicrobial and antioxidant activities of essential oils from white wormwood, rose-scented geranium and bay laurel against Salmonella typhimurium and Escherichia coli O157:H7 on fresh produce and to examine consumer acceptability of fresh produce treated with these essential oils. Our results showed that essential oil derived from rose-scented geranium exhibited the most effective antimicrobial activity at the same and similar minimum inhibition concentration levels (0...
April 11, 2016: Foods (Basel, Switzerland)
https://www.readbyqxmd.com/read/28221988/prevalence-and-phylogenetic-characterization-of-escherichia-coli-and-hygiene-indicator-bacteria-isolated-from-leafy-green-produce-beef-and-pork-obtained-from-farmers-markets-in-pennsylvania
#20
Joshua A Scheinberg, Edward G Dudley, Jonathan Campbell, Beth Roberts, Michael DiMarzio, Chitrita DebRoy, Catherine N Cutter
The popularity of farmers' markets in the United States has led to over 8,400 farmers' markets being in operation in 2015. As farmers' markets have increased in size and complexity in the kinds of foods sold at these venues, so have the potential food safety risks. Since 2008, seven major foodborne illness outbreaks and two recalls associated with food products from farmers' markets have occurred, causing 80 known reported illnesses and one death. Various researchers also have observed vendors performing high-risk food safety retail behaviors, and others have identified microbiological hazards in foods sold at farmers' markets...
February 2017: Journal of Food Protection
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