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https://www.readbyqxmd.com/read/28092761/chlorinated-and-ultraviolet-radiation-treated-reclaimed-irrigation-water-is-the-source-of-aeromonas-found-in-vegetables-used-for-human-consumption
#1
Fadua Latif-Eugenín, Roxana Beaz-Hidalgo, Carolina Silvera-Simón, Xavi Fernandez-Cassi, María J Figueras
Wastewater is increasingly being recognized as a key water resource, and reclaimed water (or treated wastewater) is used for irrigating vegetables destined for human consumption. The aim of the present study was to determine the diversity and prevalence of Aeromonas spp. both in reclaimed water used for irrigation and in the three types of vegetables irrigated with that water. Seven of the 11 (63.6%) samples of reclaimed water and all samples of vegetables were positive for the presence of Aeromonas. A total of 216 Aeromonas isolates were genotyped and corresponded to 132 different strains that after identification by sequencing the rpoD gene belonged to 10 different species...
January 13, 2017: Environmental Research
https://www.readbyqxmd.com/read/28089465/differential-bioaccumulation-patterns-of-nanosized-and-dissolved-silver-in-a-land-snail-achatina-fulica
#2
Yuanzhen Chen, Youbin Si, Dongmei Zhou, Fei Dang
With the increasing application in antimicrobial products, silver nanoparticles (AgNP) are inevitably released into the terrestrial environment, and pose potential risks to invertebrates such as land snails Achatina fulica, which take up AgNP from food and water. Here we differentiate Ag uptake biodynamic between Ag forms (i.e., PVP-AgNP vs. AgNO3) and between exposure pathways. Snails assimilated Ag efficiently from lettuce leaves pre-exposed to AgNP, with assimilation efficiencies (AEs) averaging 62-85% and food ingestion rates of 0...
January 12, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28078051/complete-genome-sequence-of-pseudomonas-brassicacearum-strain-l13-6-12-a-biological-control-agent-from-the-rhizosphere-of-potato
#3
Christin Zachow, Henry Müller, Jana Monk, Gabriele Berg
Pseudomonas brassicacearum strain L13-6-12 is a rhizosphere colonizer of potato, lettuce and sugar beet. Previous studies have shown that this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against different phytopathogens. Here, we announce and describe the complete genome sequence of P. brassicacearum L13-6-12 consisting of a single 6.7 Mb circular chromosome that consists of 5773 protein coding genes and 85 RNA-only encoding genes. Genome analysis revealed genes encoding specialized functions for pathogen suppression, thriving in the rhizosphere and interacting with eukaryotic organisms...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28076772/impact-of-dairy-manure-pre-application-treatment-on-manure-composition-soil-dynamics-of-antibiotic-resistance-genes-and-abundance-of-antibiotic-resistance-genes-on-vegetables-at-harvest
#4
Yuan-Ching Tien, Bing Li, Tong Zhang, Andrew Scott, Roger Murray, Lyne Sabourin, Romain Marti, Edward Topp
Manuring ground used for crop production is an important agricultural practice. Should antibiotic-resistant enteric bacteria carried in the manure be transferred to crops that are consumed raw, their consumption by humans or animals will represent a route of exposure to antibiotic resistance genes. Treatment of manures prior to land application is a potential management option to reduce the abundance of antibiotic resistance genes entrained with manure application. In this study, dairy manure that was untreated, anaerobically digested, mechanically dewatered or composted was applied to field plots that were then cropped to lettuce, carrots and radishes...
January 8, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28074369/mitigating-cadmium-accumulation-in-greenhouse-lettuce-production-using-biochar
#5
Ruilun Zheng, Guoxin Sun, Cui Li, Brian J Reid, Zubin Xie, Bo Zhang, Qinghai Wang
Greenhouse experiments were conducted to investigate the influence of rice straw biochar (RSB) on soil cadmium (Cd) availability and accumulation in lettuce. The RSB was applied either in bands or broadcast in the test site of four greenhouses with soil Cd concentrations ranging from 1.70-3.14 μg g(-1). Biochar doses applied in bands were half of those broadcast. The Cd levels in the shoots of lettuce were observed to be reduced by up to 57% with increasing RSB application rate (0, 6, 12, 18 t ha(-1))...
January 10, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28070585/effect-of-proline-on-biochemical-and-molecular-mechanisms-in-lettuce-lactuca-sativa-l-exposed-to-uv-b-radiation
#6
Ozkan Aksakal, Dilruba Tabay, Aslıhan Esringu, Feyza Icoglu Aksakal, Nevzat Esim
The purpose of the present study was to evaluate the role of proline (Pro) in relieving UV-B radiation-induced oxidative stress in lettuce. Lettuce seedlings were exposed to 3.3 W m(-2) UV-B radiation for 12 h after pre-treatment sprayed with 20 mM Pro. The data for malondialdehyde (MDA), hydrogen peroxide (H2O2), endogenous Pro level, the activities of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD)], total phenolic concentration, antioxidant capacity, expression of phenylalanine ammonia lyase (PAL), γ-tocopherol methyltransferase (γ-TMT) and proline dehydrogenase (ProDH) genes, phytohormone levels such as abscisic acid (ABA), gibberellic acid (GA), indole acetic acid (IAA) and salicylic acid (SA), soluble sugars and organic acids were recorded...
January 10, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28070317/prevention-of-microbial-hazard-on-fresh-cut-lettuce-through%C3%A2-adoption-of-food-safety-and-hygienic-practices-by%C3%A2-lettuce-farmers
#7
Lateefah A Oyinlola, Adewale O Obadina, Adebukunola M Omemu, Olusola B Oyewole
Lettuce is consumed raw in salads and is susceptible to microbial contamination through environment, agricultural practices, and its morphology, thus, a potential vehicle for food-borne illness. This study investigated the effect of adoption of food safety and hygienic practices by lettuce farmers on the microbial safety of field sourced lettuce in Lagos State, Nigeria. Ten structured questionnaires were administered randomly to 10 lettuce farmers to assess food safety and hygienic practices (FSH). Two farmers who practice FSH and two farmers who do not practice NFSH were finally used for this study...
January 2017: Food Science & Nutrition
https://www.readbyqxmd.com/read/28059505/predicting-plant-invasions-following-china-s-water-diversion-project
#8
Dasheng Liu, Rui Wang, Doria R Gordon, Xihua Sun, Lu Chen, Yanwen Wang
China's South to North Water Diversion (SNWD) project connects portions of the Yangtze River in the south to the Yellow River system in the north, overcoming biogeographic barriers to water movement. The diversion will supply potable water to over 110 million people and provide multiple other socio-economic benefits. However, an inadvertent negative impact of this connection includes creation of conduits for species invasions. Alligator weed (Alternanthera philoxeroides), water hyacinth (Eichhornia crassipes), and water lettuce (Pistia stratiotes) are the only aquatic plant species on China's shortlists for special control...
January 6, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/28051328/a-single-step-enrichment-medium-for-nonchromogenic-isolation-of-healthy-and-cold-injured-salmonella-spp-from-fresh-vegetables
#9
Hong-Seok Kim, Dasom Choi, Il-Byeong Kang, Dong-Hyeon Kim, Jin-Hyeok Yim, Young-Ji Kim, Jung-Whan Chon, Deog-Hwan Oh, Kun-Ho Seo
Culture-based detection of nontyphoidal Salmonella spp. in foods requires at least four working days; therefore, new detection methods that shorten the test time are needed. In this study, we developed a novel single-step Salmonella enrichment broth, SSE-1, and compared its detection capability with that of commercial single-step ONE broth-Salmonella (OBS) medium and a conventional two-step enrichment method using buffered peptone water and Rappaport-Vassiliadis soy broth (BPW-RVS). Minimally processed lettuce samples were artificially inoculated with low levels of healthy and cold-injured Salmonella Enteritidis (10(0) or 10(1) colony-forming unit/25 g), incubated in OBS, BPW-RVS, and SSE-1 broths, and streaked on xylose lysine deoxycholate (XLD) agar...
January 4, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28039776/far-red-light-is-needed-for-efficient-photochemistry-and-photosynthesis
#10
Shuyang Zhen, Marc W van Iersel
The efficiency of monochromatic light to drive photosynthesis drops rapidly at wavelengths longer than 685nm. The photosynthetic efficiency of these longer wavelengths can be improved by adding shorter wavelength light, a phenomenon known as the Emerson enhancement effect. The reverse effect, the enhancement of photosynthesis under shorter wavelength light by longer wavelengths, however, has not been well studied and is often thought to be insignificant. We quantified the effect of adding far-red light (peak at 735nm) to red/blue or warm-white light on the photosynthetic efficiency of lettuce (Lactuca sativa)...
December 15, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28035839/high-performing-windowfarm-hydroponic-system-transcriptomes-of-fresh-produce-and-microbial-communities-in-response-to-beneficial-bacterial-treatment
#11
Seungjun Lee, Ruisheng An, Parwinder Grewal, Zhongtang Yu, Zuzana Borherova, Jiyoung Lee
Beneficial microorganisms play an important role in enhancing plant health, especially by promoting resistance to plant pathogen infection. The purpose of this study was to gain an understanding of such protection by i) examining the responses of fresh produce (lettuce) to beneficial treatments in their transcriptomes, ii) comparing biological (bacteria, fungi, and oomycete) communities and their diversity when treated with Pseudomonas chlororaphis (beneficial bacterium) in windowfarm hydroponic systems, and iii) identifying the microorganisms in root areas and water...
December 2016: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28034504/revisiting-quechers-parameters-for-sample-preparation-in-pesticide-residue-analysis-of-lettuce-by-liquid-chromatography-tandem-mass-spectrometry
#12
Fernanda Ribeiro Begnini Konatu, Márcia Cristina Breitkreitz, Isabel Cristina Sales Fontes Jardim
A new analytical method using a quick, easy, cheap, effective, rugged, and safe (QuEChERS) procedure for multi-residue determination of 16 multiclass pesticides in five different types of lettuce was developed using high-performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry. The QuEChERS procedure was optimized in terms of extractor solvent, partitioning salts and clean-up salts, through recovery, gravimetric analysis and matrix-effect studies. Microwave extraction and the use of disposable pipette extraction in the clean-up step were also tested, providing interesting alternatives to the traditional QuEChERS method, depending on the pesticides properties...
December 21, 2016: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28033096/detection-of-heavy-metals-in-common-vegetables-at-vara%C3%A5-din-city-market-croatia
#13
Zvjezdana Stančić, Dinko Vujević, Ana Gomaz, Saša Bogdan, Dragutin Vincek
The present study was aimed at the estimation of heavy metal content in vegetables sold at the city market of one of the densely populated Croatian cities, Varaždin, and to establish the relationship between their levels and possible sources of contamination. Twenty-eight samples of the most common diet vegetables (red and white potato, onion, carrot, common bean, lettuce, and cabbage) were randomly bought at the market in September and October 2013. Using the atomic absorption spectrometry method, concentrations of nine heavy metals (As, Cd, Cr, Cu, Hg, Mn, Ni, Pb, and Zn) were measured in the selected samples...
December 1, 2016: Arhiv za Higijenu Rada i Toksikologiju
https://www.readbyqxmd.com/read/28030923/microbiological-characterization-of-vegetables-and-their-rhizosphere-soil-in-eastern-poland
#14
Teresa Kłapeć, Angelina Wójcik-Fatla, Alicja Cholewa, Grażyna Cholewa, Jacek Dutkiewicz
The aim of this study was to investigate the bacteriological quality of 5 kinds of vegetables (lettuce, dill, radish, beetroot, carrot) and their rhizosphere soil, originating from conventional farms located in the Lublin Province of Eastern Poland. A total number of 35 samples of fresh vegetables (FV) taken immediately from soil, 35 samples of soil from rhizosphere of these vegetables (SR) and 35 samples of vegetables sold at retail in the markets in Lublin (VR) were examined. The samples were analysed for the content of: aerobic mesophilic bacteria (AMB) grown at 30°C and 37°C, Gram-negative bacteria of Enterobacteriaceae family, faecal coliform (FC) bacteria, Salmonella spp...
23, 2016: Annals of Agricultural and Environmental Medicine: AAEM
https://www.readbyqxmd.com/read/28028698/a-dose-response-relationship-for-marketable-yield-reduction-of-two-lettuce-lactuca-sativa-l-cultivars-exposed-to-tropospheric-ozone-in-southern-europe
#15
Riccardo Marzuoli, Angelo Finco, Maria Chiesa, Giacomo Gerosa
The present study investigated the response to ozone (O3) of two cultivars (cv.'Romana' and cv. 'Canasta') of irrigated lettuce grown in an open-top chamber (OTC) experiment in Mediterranean conditions. Two different levels of O3 were applied, ambient O3 in non-filtered OTCs (NF-OTCs) and -40% of ambient O3 in charcoal-filtered OTCs (CF-OTCs), during four consecutive growing cycles. At the end of each growing cycle, the marketable yield (fresh biomass) was assessed while during the growing periods, measurements of the stomatal conductance at leaf level were performed and used to define a stomatal conductance model for calculation of the phytotoxic ozone dose (POD) absorbed by the plants...
December 27, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28024451/weathering-in-soil-increases-nanoparticle-cuo-bioaccumulation-within-a-terrestrial-food-chain
#16
Alia D Servin, Luca Pagano, Hiram Castillo-Michel, Roberto De la Torre-Roche, Joseph Hawthorne, Jose A Hernandez-Viezcas, René Loredo-Portales, Sanghamitra Majumdar, Jorge Gardea-Torresday, Om Parkash Dhankher, Jason C White
This study evaluates the bioaccumulation of unweathered (U) and weathered (W) CuO in NP, bulk and ionic form (0-400 mg/kg) by lettuce exposed for 70 d in soil co-contaminated with field incurred chlordane. To evaluate CuO trophic transfer, leaves were fed to crickets (Acheta domestica) for 15 d, followed by insect feeding to lizards (Anolis carolinensis). Upon weathering, the root Cu content of the NP treatment increased 214% (327 ± 59.1 mg/kg) over unaged treatment. Cu root content decreased in bulk and ionic treatments from 70-130 mg/kg to 13-26 mg/kg upon aging in soil...
December 26, 2016: Nanotoxicology
https://www.readbyqxmd.com/read/28018414/mads-box-genes-and-gibberellins-regulate-bolting-in-lettuce-lactuca-sativa-l
#17
Yingyan Han, Zijing Chen, Shanshan Lv, Kang Ning, Xueliang Ji, Xueying Liu, Qian Wang, Renyi Liu, Shuangxi Fan, Xiaolan Zhang
Bolting in lettuce is promoted by high temperature and bolting resistance is of great economic importance for lettuce production. But how bolting is regulated at the molecular level remains elusive. Here, a bolting resistant line S24 and a bolting sensitive line S39 were selected for morphological, physiological, transcriptomic and proteomic comparisons. A total of 12204 genes were differentially expressed in S39 vs. S24. Line S39 was featured with larger leaves, higher levels of chlorophyll, soluble sugar, anthocyanin and auxin, consistent with its up-regulation of genes implicated in photosynthesis, oxidation-reduction and auxin actions...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28012907/culex-melanoconion-panocossa-from-peninsular-florida-usa
#18
Erik M Blosser, Nathan D Burkett-Cadena
Culex (Melanoconion) panocossa is a suspected vector of Venezuelan equine encephalitis virus in Central America. Prior to this report, Cx. panocossa was known from Central America (Belize, Costa Rica, El Salvador, Guatemala, southern Mexico, Panama), northern South America (Colombia, Venezuela) and the Greater Antilles (Cuba and Jamaica). Larvae (n=5) and adults (n=4286) of Cx. panocossa were collected at two locations near Homestead, FL, which indicates substantial established populations of this probable vector species in the continental US...
December 21, 2016: Acta Tropica
https://www.readbyqxmd.com/read/28000070/accumulation-of-trace-elements-in-edible-crops-and-poplar-grown-on-a-titanium-ore-landfill
#19
Mohamad Assad, Fabienne Tatin-Froux, Damien Blaudez, Michel Chalot, Julien Parelle
Urban gardening has recently experienced rapid development; however, the risk of the transfer of toxic elements from neighboring industry needs to be evaluated. We performed a multi-elemental analysis with several common edible crops (cucumber, pepper, cabbage, and lettuce) and poplar grown directly on a titanium ore landfill as a maximized scenario of exposure. Despite elevated concentrations of soil Ca, Fe, Mn, and Ti resulting from the industrial process, we did not register higher accumulation of these elements in the edible parts of crops or in poplar leaves grown on red gypsum compared with the control soil...
December 20, 2016: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/27995654/simultaneous-detection-of-sulfoxaflor-and-its-metabolites-x11719474-and-x11721061-in-lettuce-using-a-modified-quechers-extraction-method-and-liquid-chromatography-tandem-mass-spectrometry
#20
Sung-Woo Kim, Md Musfiqur Rahman, A M Abd El-Aty, Md Humayun Kabir, Tae Woong Na, Jeong-Heui Choi, Ho-Chul Shin, Jae-Han Shim
An analytical method has been developed to quantify the residual levels of sulfoxaflor and its metabolites (X11719474 and X11721061) in/on cultivated lettuce grown under greenhouse conditions. Samples were extracted and purified using a quick, easy, cheap, effective, rugged, and safe 'QuEChERS' method (original version) following systematic method optimization and were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Good linearity with coefficient of determination ≥0.9930 was obtained and the limits of detection (LOD) and quantification (LOQ) were in the ranges of 0...
November 5, 2016: Biomedical Chromatography: BMC
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