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Merete Wiken Dees, May Bente Brurberg, Erik Lysøe
The genus Microbacterium contains bacteria that are ubiquitously distributed in various environments and includes plant-associated bacteria that are able to colonize tissue of agricultural crop plants. Here, we report the 3,508,491 bp complete genome sequence of Microbacterium sp. strain BH-3-3-3, isolated from conventionally grown lettuce (Lactuca sativa) from a field in Vestfold, Norway. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession CP017674.
March 2017: Genomics Data
Phasita Toonsiri, Stephen J Del Grosso, Arina Sukor, Jessica G Davis
Improper application of nitrogen (N) fertilizer and environmental factors can cause the loss of nitrous oxide (NO) to the environment. Different types of fertilizers with different C/N ratios may have different effects on the environment. The focus of this study was to evaluate the effects of environmental factors and four organic fertilizers (feather meal, blood meal, fish emulsion, and cyano-fertilizer) applied at different rates (0, 28, 56, and 112 kg N ha) on NO emissions and to track CO emissions from a lettuce field ( L...
November 2016: Journal of Environmental Quality
Steffen Hadasch, Ivan Simko, Ryan J Hayes, Joseph O Ogutu, Hans-Peter Piepho
Breeding for traits with polygenic inheritance is a challenging task that can be done by phenotypic selection, marker-assisted selection (MAS) or genome-wide selection. We comparatively evaluated the predictive abilities of four selection models on a biparental lettuce ( L.) population genotyped with 95 single nucleotide polymorphisms and 205 amplified fragment length polymorphism markers. These models were based on (i) phenotypic selection, (ii) MAS (with quantitative trait locus (QTL)-linked markers), (iii) genomic prediction using all the available molecular markers, and (iv) genomic prediction using molecular markers plus QTL-linked markers as fixed covariates...
March 2016: Plant Genome
Willeke de Bruin, Chris van der Merwe, Quenton Kritzinger, Riana Bornman, Lise Korsten
It has long been understood that the presence of endocrine disrupter chemicals (EDCs) in water can affect the reproductive, behavioural and regulatory systems of different types of mammals. Thus far, only a handful of studies have examined its impact on plant systems. Present research is limited to the potential uptake of these chemicals by plants and the general phytotoxic effects it can elicit. The aim of this study was to determine what effect an EDC has on developing plant and cell organelles and how it affects it...
November 24, 2016: Chemosphere
Michelle S F Tan, Sadequr Rahman, Gary A Dykes
This study investigated the removal of bacterial surface structures, particularly flagella, using sonication, and examined its effect on the attachment of Salmonella Typhimurium ATCC 14028 cells to plant cell walls. S. Typhimurium ATCC 14028 cells were subjected to sonication at 20 kHz to remove surface structures without affecting cell viability. Effective removal of flagella was determined by staining flagella of sonicated cells with Ryu's stain and enumerating the flagella remaining by direct microscopic counting...
April 2017: Food Microbiology
Bei Zhang, Madhusudhan Rapolu, Sandeep Kumar, Manju Gupta, Zhibin Liang, Zhenlin Han, Philip Williams, Wei Wen Su
A novel approach is developed for coordinated expression of multiple proteins from a single transgene in plants. An Ssp DnaE mini-intein variant engineered for hyper N-terminal autocleavage is covalently linked to the foot-and-mouth disease virus 2A (F2A) peptide with unique ribosome skipping property, via a peptide linker, to create an "IntF2A" self-excising fusion protein domain. This IntF2A domain acts, in cis, to direct highly effective release of its flanking proteins of interest (POI) from a "polyprotein" precursor in plants...
November 23, 2016: Plant Biotechnology Journal
Anna Czubacka, Ermanno Sacco, Hanna Olszak-Przybyś, Teresa Doroszewska
Genetic transformation of plants allows us to obtain improved genotypes enriched with the desired traits. However, if transgenic lines were to be used in breeding programs the stability of inserted transgenes is essential. In the present study, we followed the inheritance of transgenes in hybrids originated from crossing two transgenic tobacco lines resistant to Potato virus Y (PVY): MN 944 LMV with the transgene containing Lettuce mosaic virus coat protein gene (LMV CP) and AC Gayed ROKY2 with PVY replicase gene (ROKY2)...
November 23, 2016: Journal of Applied Genetics
Merete Wiken Dees, May Bente Brurberg, Erik Lysøe
Here, we present the 3,795,952 bp complete genome sequence of the biofilm-forming Curtobacterium sp. strain BH-2-1-1, isolated from conventionally grown lettuce (Lactuca sativa) from a field in Vestfold, Norway. The nucleotide sequence of this genome was deposited into NCBI GenBank under the accession CP017580.
December 2016: Genomics Data
Peng Zhang, Yuhui Ma, Shutong Liu, Guohua Wang, Junzhe Zhang, Xiao He, Jing Zhang, Yukui Rui, Zhiyong Zhang
Toxicity and uptake of nano-CeO2 (nCeO2) in edible vegetables are not yet fully understood. In the present study, we grew romaine lettuce in sand amended with nCeO2. At high concentrations (1000 and 2000 mg/kg), nCeO2 diminished the chlorophyll content by 16.5% and 25.8%, respectively, and significantly inhibited the biomass production. nCeO2 (≥100 mg/kg) altered antioxidant enzymatic activities and malondialdehyde levels in the plants. nCeO2 (≥500 mg/kg) triggered a remarkable increase of nitrate-N level in the shoots, which can be converted to toxic nitrite in humans thereby posed risk to human health...
November 11, 2016: Environmental Pollution
Abhinav Mishra, Hao Pang, Robert L Buchanan, Donald W Schaffner, Abani K Pradhan
: Majority of foodborne outbreaks in the U.S. associated with the consumption of leafy greens contaminated with E. coli O157:H7 have been reported during July-November. A dynamic system model consisting of subsystems and inputs to the system (soil, irrigation, cattle, wild pig, and rainfall) simulating a hypothetical farm was developed. The model assumed two crops of lettuce in a year, and simulated planting, irrigation, harvesting, ground preparation for the new crop, contamination of soil and plants, and survival of E...
November 11, 2016: Applied and Environmental Microbiology
Krin S Mann, Ralf G Dietzgen
RNA silencing in plants can be triggered by the introduction of an exogenous gene. Green fluorescent protein (GFP) has been widely used as a visual reporter to study RNA silencing and viral-mediated suppression of RNA silencing in the model plant Nicotiana benthamiana. In transgenic N. benthamiana plants expressing an endoplasmic reticulum targeted GFP variant (16c) known as mGFP5, RNA silencing can be induced by ectopic over-expression of mGFP5. However, other GFP variants can also be used to induce GFP silencing in these plants...
January 2017: Journal of Virological Methods
Ramalingam Radhakrishnan, Jae-Man Park, In-Jung Lee
Very few bacterial species were identified as bio-herbicides for weed control. The present research was focused to elucidate the plant growth retardant properties of Enterobacter sp. I-3 during their interaction by determining the changes in endogenous photosynthetic pigments, plant hormones and amino acids. The two bacterial isolates I-4-5 and I-3 were used to select the superior bacterium for controlling weed seeds (Echinochloa crus-galli L. and Portulaca oleracea L.) germination. The post-inoculation of I-3 (Enterobacter sp...
December 2016: Microbiological Research
Bokai Zhu, Qinglin Chen, Songcan Chen, Yong-Guan Zhu
The demand for organic food products, especially for organic vegetables has been growing rapidly in the last few decades. However, the risk of introducing more antibiotic resistant genes (ARGs) and antibiotic resistant bacteria (ARB) to the vegetables by organic production procedures has long been overlooked. In our study, by using high-throughput quantitative PCR and 16sRNA Illumina sequencing technology, we investigated the abundance and diversity of ARGs and the microbial communities in conventionally (CPL) and organically produced lettuce (OPL)...
November 4, 2016: Environment International
A A Tikhomirov, S V Trifonov, E A Morozov, Yu A Kudenko, G S Kalacheva, S A Ushakova
Methods of physicochemical further oxidation of hardly soluble sediment obtained from "wet combustion" of human exometabolites applied to space-purpose Bio Technological Life Support Systems (BTLLS) were studied. Most hardly dissoluble sediment containing Ca, P, Mg, and other essential plant nutrition elements were shown to dissolve in H2O2 and HNO3 aqueous media activated by alternating electric current. Dissolved additional mineral elements allowed (as demonstrated for lettuce) to increase the productivity of BTLLS phototrophic unit plants more than twice, which is comparable to their productivity on standard Knop solution with balanced chemical composition...
September 2016: Doklady. Biochemistry and Biophysics
Mojgan Yazdi, Masoud Yavarmanesh, Masumeh Bahreini, Mohebbat Mohebbi
The aim of this research was to preliminary track fecal source male-specific F(+)RNA coliphages including human and animals in lettuce. At first, two published virus extraction procedures of ultracentrifugation and PEG precipitation were compared using DAL assay for determining the recovery efficiency in lettuce spiked artificially with three concentrations (10(2), 10(4), 10(6) pfu/100 ml) of MS2 coliphage. The results showed that PEG precipitation had the highest recovery in which the recovery efficiency at the spiked level of 10(6) pfu/100 ml was 16...
November 4, 2016: Food and Environmental Virology
Volha Lazouskaya, Taozhu Sun, Li Liu, Gang Wang, Yan Jin
Bacterial contamination of fresh produce is a growing concern in food industry. Pathogenic bacteria can attach to and colonize the surfaces of fresh produce and cause disease outbreaks among consumers. Surface properties of both bacteria and produce affect bacterial contamination; however, the effects of produce roughness, topography, and hydrophobicity on bacterial retention are still poorly understood. In this work, we used spherical polystyrene colloids as bacterial surrogates to investigate colloid retention on and removal (by rinsing) from fresh produce surfaces including tomato, orange, apple, lettuce, spinach, and cantaloupe, and from surrogate produce surface Sharklet (a micro-patterned polymer)...
November 3, 2016: Journal of Food Science
Gregory E Welbaum, Osamah S Khan, Nezar H Samarah
Thermogradient tables were first developed in the 1950s primarily to test seed germination over a range of temperatures simultaneously without using a series of incubators. A temperature gradient is passively established across the surface of the table between the heated and cooled ends and is lost quickly at distances above the surface. Since temperature is only controlled on the table surface, experiments are restricted to shallow containers, such as Petri dishes, placed on the table. Welding continuous aluminum vertical strips or gussets perpendicular to the surface of a table enables temperature control in depth via convective heat flow...
October 22, 2016: Journal of Visualized Experiments: JoVE
Luise Müller, Lasse Dam Rasmussen, Tenna Jensen, Anna Charlotte Schultz, Charlotte Kjelsø, Celine Barnadas, Kim Sigsgaard, Anne Ribert Larsen, Carl Widstrup Jensen, Simon Jeppesen, Katrine Uhrbrand, Nikolas Hove, Kåre Mølbak, Steen Ethelberg
INTRODUCTION: In early April 2016, an unusual high number of point-source outbreaks of gastrointestinal disease were reported to occur in Denmark. METHODS: Outbreaks were individually investigated. Two analytical studies were performed. Patient stool samples collected and analysed; positive stool samples were sequenced over the polymerase and/or capsid gene areas. Implicated lettuce heads were collected and analysed for the presence of norovirus. Foods were traced-back and traced-forward and international alert systems applied...
October 4, 2016: PLoS Currents
Sylwester Smoleń, Iwona Kowalska, Małgorzata Czernicka, Mariya Halka, Kinga Kęska, Włodzimierz Sady
Iodine (I) and selenium (Se) are included in the group of beneficial elements. They both play important roles in humans and other animals, particularly in the regulation of thyroid functioning. A substantial percentage of people around the world suffer from health disorders related to the deficiency of these elements in the diet. Salicylic acid (SA) is a compound similar to phytohormones and is known to improve the efficiency of I biofortification of plants. The influence of SA on Se enrichment of plants has not, however, been recognized together with its effect on simultaneous application of I and Se to plants...
2016: Frontiers in Plant Science
Sofia V Poimenidou, Danai-Natalia Chatzithoma, George-John Nychas, Panagiotis N Skandamis
Pathogens found on fresh produce may encounter low temperatures, high acidity and limited nutrient availability. The aim of this study was to evaluate the effect of habituation of Listeria monocytogenes on cherry tomatoes or lettuce leaves on its subsequent response to inhibitory levels of acid, osmotic and heat stress. Habituation was performed by inoculating lettuce coupons, whole cherry tomatoes or tryptic soy broth (TSB) with a three-strains composite of L. monocytogenes, which were further incubated at 5°C for 24 hours or 5 days...
2016: PloS One
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