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https://www.readbyqxmd.com/read/28342799/lost-in-processing-perceived-healthfulness-taste-and-caloric-content-of-whole-and-processed-organic-food
#1
Marília Prada, Margarida V Garrido, David Rodrigues
The "organic" claim explicitly informs consumers about the food production method. Yet, based on this claim, people often infer unrelated food attributes. The current research examined whether the perceived advantage of organic over conventional food generalizes across different organic food types. Compared to whole organic foods, processed organic foods are less available, familiar and prototypical of the organic food category. In two studies (combined N = 258) we investigated how both organic foods types were perceived in healthfulness, taste and caloric content when compared to their conventional alternatives...
March 23, 2017: Appetite
https://www.readbyqxmd.com/read/28337397/development-and-application-of-a-brush-spray-derived-from-a-calligraphy-brush-style-synthetic-hair-pen-for-use-in-esi-ms
#2
Jen-Ying Liu, Pei-Chun Chen, Yea-Wenn Liou, Kai-Yin Chang, Cheng-Huang Lin
The development of a novel type of a sampling/ionization kit for use in electrospray ionization/mass spectrometry is reported. Using a small calligraphy-brush-style synthetic hair pen (nylon-brush), and analogous to paper-spray mass spectrometry, the analytes can be collected, elution/desorption and then ionized from the surface of the nylon-brush. The body of the kit was produced by means of a commercial 3D-printer, in which ABS (acrylonitrile butadiene styrene) was used as the starting material. Meanwhile, a small nylon-brush was embedded inside a 3D-printed plastic cell, in which a solvent was supplied to rinse the brush by means of capillary action...
2017: Mass Spectrometry
https://www.readbyqxmd.com/read/28335468/differential-degradation-and-detoxification-of-an-aromatic-pollutant-by-two-different-peroxidases
#3
Aysha Hamad Alneyadi, Iltaf Shah, Synan F AbuQamar, Syed Salman Ashraf
Enzymatic degradation of organic pollutants is a new and promising remediation approach. Peroxidases are one of the most commonly used classes of enzymes to degrade organic pollutants. However, it is generally assumed that all peroxidases behave similarly and produce similar degradation products. In this study, we conducted detailed studies of the degradation of a model aromatic pollutant, Sulforhodamine B dye (SRB dye), using two peroxidases-soybean peroxidase (SBP) and chloroperoxidase (CPO). Our results show that these two related enzymes had different optimum conditions (pH, temperature, H₂O₂ concentration, etc...
March 18, 2017: Biomolecules
https://www.readbyqxmd.com/read/28330287/transformation-of-lactuca-sativa-l-with-rol-c-gene-results-in-increased-antioxidant-potential-and-enhanced-analgesic-anti-inflammatory-and-antidepressant-activities-in-vivo
#4
Hammad Ismail, Erum Dilshad, Mohammad Tahir Waheed, Moniba Sajid, Waqas Khan Kayani, Bushra Mirza
Lettuce is an important edible crop which possesses various medicinal properties. In this study Lactuca sativa L. (cv Grand Rapids) was transformed by Agrobacterium-mediated transformation with rol C gene. Transgene integration and expression was confirmed through PCR and semiquantitative RT-PCR. The transformed extracts were evaluated for their in vitro antioxidant and in vivo analgesic, anti-inflammatory and antidepressant activities in rats. The transformed plants showed 53-98 % increase in total phenolic and 45-58 % increase in total flavonoid contents compared with untransformed plants...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28319746/predicting-biphasic-responses-in-binary-mixtures-pelargonic-acid-versus-glyphosate
#5
Regina G Belz, Hans-Peter Piepho
Predicting hormesis in mixtures is challenging, but essential considering that chemical exposures often occur in mixtures and at low doses. This study investigated mixture effects with two herbicides prone to induce hormesis and to interact, namely pelargonic acid versus glyphosate. Five independent mixture experiments were conducted in vitro to assess effects on root growth of lettuce. Mixture effects on the dose were analyzed using classical joint-action models in terms of deviation from the reference model of concentration addition...
March 13, 2017: Chemosphere
https://www.readbyqxmd.com/read/28314158/asparagus-decline-autotoxicity-and-autotoxic-compounds-in-asparagus-rhizomes
#6
Hisashi Kato-Noguchi, Keisuke Nakamura, Osamu Ohno, Kiyotake Suenaga, Nobuyuki Okuda
Asparagus (Asparagus officinalis L.) is a perennial vegetable, but its crop productivity and quality decrease gradually. One possible reason for "asparagus decline" is thought to be the autotoxicity of asparagus. However, the autotoxic property of asparagus rhizomes remains unknown. The objective of this study was to determine the potential role of rhizomes in the autotoxicity of asparagus. An aqueous methanol extract of asparagus rhizomes inhibited the growth of asparagus seedlings and six other test plants in a concentration-dependent manners: garden cress (Lepidum sativum L...
March 1, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/28314134/phytotoxicity-of-effluents-from-swine-slaughterhouses-using-lettuce-and-cucumber-seeds-as-bioindicators
#7
Michel David Gerber, Thomaz Lucia, Luciara Correa, José Eduardo Pereira Neto, Érico Kunde Correa
This study evaluated the phytotoxic effects of raw and treated effluents from a swine slaughterhouse on cucumber and lettuce seeds and determined correlations among physicochemical characteristics of such effluents and the germination of seeds used as bioindicators. Physicochemical parameters were characterized for both effluents and their phytotoxicity was determined through the germination index (GI), the root length (RL) and the number of germinated seeds (SG) for both plant species. The effluents treatment system was efficient to reduce the concentration of some physicochemical parameters to levels within those recommended by the Brazilian legislation, except for P, ammoniacal N and TKN concentration...
March 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28303392/lettuce-flavonoids-screening-and-phenotyping-by-chlorophyll-fluorescence-excitation-ratio
#8
Marek Zivcak, Klaudia Brückova, Oksana Sytar, Marian Brestic, Katarina Olsovska, Suleyman I Allakhverdiev
Environmentally induced variation and the genotypic differences in flavonoid and phenolic content in lettuce can be reliably detected using the appropriate parameters derived from the records of rapid non-invasive fluorescence technique. The chlorophyll fluorescence excitation ratio method was designed as a rapid and non-invasive tool to estimate the content of UV-absorbing phenolic compounds in plants. Using this technique, we have assessed the dynamics of accumulation of flavonoids related to developmental changes and environmental effects...
March 16, 2017: Planta
https://www.readbyqxmd.com/read/28299485/characterization-of-lettuce-big-vein-associated-virus-and-mirafiori-lettuce-big-vein-virus-infecting-lettuce-in-saudi-arabia
#9
M Umar, M A Amer, M A Al-Saleh, I M Al-Shahwan, M T Shakeel, A M Zakri, N I Katis
During 2014 and 2015, 97 lettuce plants that showed big-vein-disease-like symptoms and seven weed plants were collected from the Riyadh region. DAS-ELISA revealed that 25% and 9% of the lettuce plants were singly infected with LBVaV and MiLBVV, respectively, whereas 63% had a mixed infection with both viruses. The results were confirmed by multiplex reverse transcription polymerase chain reaction using primers specific for LBVaV and MiLBVV. LBVaV and MiLBVV were also detected in Sonchus oleraceus and Eruca sativa, respectively...
March 16, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28298675/lactic-acid-as-potential-substitute-of-acetic-acid-for-dissolution-of-chitosan-preharvest-application-to-butterhead-lettuce
#10
María Gabriela Goñi, Bárbara Tomadoni, Sara Inés Roura, María Del Rosario Moreira
Chitosan must be dissolved in acid solution to activate its antimicrobial properties. The objectives of present study were to determine whether acetic and lactic acids used to dissolve chitosan would influence its effectiveness to control the native microflora of Butterhead lettuce at harvest and during postharvest storage (7-8 °C, 5 days). Chitosan was applied as a SINGLE DOSE (14, 10, 7, 3 or 0 days previous to harvest) or in SUCCESSIVE DOSES (at 14 + 10 + 7+3 + 0 days prior to harvest). Although chitosan in acetic acid showed antimicrobial activity, treated plants showed dried brown stains which significantly reduced sensorial quality...
March 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28289987/biochar-based-constructed-wetlands-to-treat-reverse-osmosis-rejected-concentrates-in-chronic-kidney-disease-endemic-areas-in-sri-lanka
#11
B C L Athapattu, T W L R Thalgaspitiya, U L S Yasaratne, Meththika Vithanage
The objectives were to investigate the potential remedial measures for reverse osmosis (RO) rejected water through constructed wetlands (CWs) with low-cost materials in the media established in chronic kidney disease of unknown etiology (CKDu) prevalent area in Sri Lanka. A pilot-scale surface and subsurface water CWs were established at the Medawachchiya community-based RO water supply unit. Locally available soil, calicut tile and biochar were used in proportions of 81, 16.5 and 2.5% (w/w), respectively, as filter materials in the subsurface...
March 13, 2017: Environmental Geochemistry and Health
https://www.readbyqxmd.com/read/28284554/effects-of-biochar-on-reducing-the-abundance-of-oxytetracycline-antibiotic-resistance-genes-and-human-pathogenic-bacteria-in-soil-and-lettuce
#12
Manli Duan, Haichao Li, Jie Gu, Xiaxia Tuo, Wei Sun, Xun Qian, Xiaojuan Wang
Antibiotics and antibiotic resistance genes (ARGs) in soil can affect human health via the food chain. Biochar is a soil amendment but its impacts on ARGs and the microbial communities associated with soil and vegetables are unclear. Therefore, we established three lettuce pot culture experiments, i.e., O300: 300 mg/kg oxytetracycline (OTC), BO300: 300 mg/kg OTC + 2% biochar, and a control without OTC or biochar. We found that under BO300, the relative abundances of ARGs were reduced by 51.8%, 43.4%, and 44...
March 8, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28280387/ulva-lactuca-polysaccharides-prevent-wistar-rat-breast-carcinogenesis-through-the-augmentation-of-apoptosis-enhancement-of-antioxidant-defense-system-and-suppression-of-inflammation
#13
Gamal-Eldein F Abd-Ellatef, Osama M Ahmed, Eman S Abdel-Reheim, Abdel-Hamid Z Abdel-Hamid
BACKGROUND: Recently, several research studies have been focused on the isolation and function of the polysaccharides derived from different algal species, which revealed multiple biological activities such as antioxidant and antitumor activities. This study assesses the possible breast cancer chemopreventive properties of common seaweeds, sea lettuce, Ulva lactuca (ulvan) polysaccharides using in vitro bioassays on human breast cancer cell line (MCF-7) and an in vivo animal model of breast carcinogenesis...
2017: Breast Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/28278633/synthesis-of-a-new-allelopathic-agent-from-the-biotransformation-of-ent-15%C3%AE-hydroxy-16-kauren-19-oic-acid-with-fusarium-proliferatum
#14
A D Rocha, H da S Vieira, J A Takahashi, M A D Boaventura
The use of kaurane diterpenes as substrates in fungal biotransformation to achieve bioactive compounds has been widely reported. In this work, the natural product kaurenoic acid, a diterpene widely distributed in the plant Kingdom, was chemically converted into ent-15α-hydroxy-kaur-16-en-19-oic acid (1). Substrate 1 was subjected to biotransformation by the fungus Fusarium proliferatum, furnishing a new derivative, ent-2α,15α-dihydroxy-kaur-16-en-19-oic acid (2). The structure of metabolite 2 was deduced on the basis of spectroscopy and MS data...
February 28, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28273532/arsenic-uptake-by-lettuce-from-as-contaminated-soil-remediated-with-pteris-vittata-and-organic-amendment
#15
Letuzia M de Oliveira, Das Suchismita, Julia Gress, Bala Rathinasabapathi, Yanshan Chen, Lena Q Ma
Leaching of inorganic arsenic (As) from chromated copper arsenate (CCA)-treated wood may elevate soil As levels. Thus, an environmental concern arises regarding As accumulation in vegetables grown in these soils. In this study, a greenhouse experiment was conducted to investigate the ability of As-hyperaccumulator P. vittata and organic amendments in reducing As uptake by lettuce (Lactuca sativa) from a soil contaminated from CCA-treated wood (63.9 mg kg(-1) As). P. vittata was grown for 150 d in a CCA-contaminated soil amended with biochar, activated carbon or coffee grounds at 1%, followed by lettuce for another 55 d...
February 24, 2017: Chemosphere
https://www.readbyqxmd.com/read/28272449/design-and-functional-characterization-of-a-novel-abscisic-acid-analog
#16
Xiaoqiang Han, Lun Jiang, Chuanliang Che, Chuan Wan, Huizhe Lu, Yumei Xiao, Yanjun Xu, Zhongzhou Chen, Zhaohai Qin
The phytohormone abscisic acid (ABA) plays a crucial role in mediating plant growth and development by recruiting genetically redundant ABA receptors. To overcome its oxidation inactivation, we developed a novel ABA analog named 2',3'-benzo-iso-ABA (iso-PhABA) and studied its function and structural characterization with A. thaliana ABA receptors. The (+)-iso-PhABA form showed much higher ABA-like activities than (+)-ABA including inhibitory effects on the seed germination of lettuce and A. thaliana, wheat embryo germination and rice seedling elongation...
March 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28271926/listeria-monocytogenes-internalizes-in-romaine-lettuce-grown-in-greenhouse-conditions
#17
Archana G Shenoy, Haley F Oliver, Amanda J Deering
Listeria monocytogenes has been implicated in a number of outbreaks involving fresh produce, including an outbreak in 2016 resulting from contaminated packaged salads. The persistence and internalization potential of L. monocytogenes in romaine lettuce was evaluated, and the persistence of two L. monocytogenes strains was assessed on three romaine lettuce cultivars. Seeds were germinated, and plants grown in three soil types (i.e., standard potting mix, autoclaved potting mix, and top soil) and sterile soft-top agar for up to 21 days...
March 8, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28265267/bacterial-surface-displayed-gii-4-human-norovirus-capsid-proteins-bound-to-hbga-like-molecules-in-romaine-lettuce
#18
Ming Wang, Shaofeng Rong, Peng Tian, Yue Zhou, Shimin Guan, Qianqian Li, Dapeng Wang
Human Noroviruses (HuNoVs) are the main cause of non-bacterial gastroenteritis. Contaminated produce is a main vehicle for dissemination of HuNoVs. In this study, we used an ice nucleation protein mediated surface display system to present the protruding domain of GII.4 HuNoV capsid protein on bacterial surface and used it as a new strategy to explore interaction between HuNoV protein and receptor candidates from romaine lettuce. The surface-displayed HuNoV proteins were confirmed on the surface of the transformed bacteria by an immunofluorescence assay...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28262983/evaluating-the-effects-of-herbicide-drift-on-non-target-terrestrial-plants-a-case-study-with-mesotrione
#19
Richard A Brain, Jeff Perine, Catriona Cooke, Clare Butler Ellis, Paul Harrington, Andrew Lane, Christine Sullivan, Mark Ledson
Nature of exposure is a fundamental driver in non-target terrestrial plant (NTTP) risk assessment for pesticides, consequently a novel study was designed to generate field-based drift exposure and evaluate corresponding biological effects of the herbicide mesotrione. The approach used a combination of U.S. guideline drift reduction technology (DRT) and vegetative vigor approaches. In each of three independent replicate spray application trials, ten pots each of lettuce and tomato were placed at distances of 10, 20, 30, 40 and 50 ft (∼3, 6, 9, 12, and 15 m) from the downwind edge of the spray boom...
March 6, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28256413/treated-wastewater-phytotoxicity-assessment-using-lactuca%C3%A2-sativa-focus-on-germination-and-root-elongation-test-parameters
#20
Anne Priac, Pierre-Marie Badot, Grégorio Crini
Sensitive and simple ecotoxicological bioassays like seed germination and root elongation tests are commonly used to evaluate the phytotoxicity of waste and industrial discharge waters. Although the tests are performed following national and international standards, various parameters such as the number of seeds per dish, the test duration or the type of support used remain variable. To be able to make a correct comparison of results from different studies, it is crucial to know which parameter(s) could affect ecotoxicological diagnosis...
February 27, 2017: Comptes Rendus Biologies
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