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https://www.readbyqxmd.com/read/28632416/survival-of-escherichia-coli-on-lettuce-under-field-conditions-encountered-in-the-northeastern-united-states
#1
Daniel L Weller, Jasna Kovac, Sherry Roof, David J Kent, Jeffrey I Tokman, Barbara Kowalcyk, David Oryang, Renata Ivanek, Anna Aceituno, Christopher Sroka, Martin Wiedmann
Although wildlife intrusion and untreated manure have been associated with microbial contamination of produce, relatively few studies have examined the survival of Escherichia coli on produce under field conditions following contamination (e.g., via splash from wildlife feces). This experimental study was performed to estimate the die-off rate of E. coli on preharvest lettuce following contamination with a fecal slurry. During August 2015, field-grown lettuce was inoculated via pipette with a fecal slurry that was spiked with a three-strain cocktail of rifampin-resistant nonpathogenic E...
June 20, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28613869/effect-of-different-elicitors-and-pre-harvest-day-application-on-the-content-of-phytochemicals-and-antioxidant-activity-of-butterhead-lettuce-lactuca-sativa-var-capitata-produced-under-hydroponic-conditions
#2
Jesus Omar Moreno-Escamilla, Emilio Alvarez-Parrilla, Laura A de la Rosa, José Alberto Núñez-Gastélum, Gustavo A González-Aguilar, Joaquin Rodrigo-Garcia
The effect of four elicitors on phytochemical content in two varieties of lettuce was evaluated. The best pre-harvest day for application of each elicitor was chosen. Solutions of arachidonic acid (AA), salicylic acid (SA), methyl jasmonate (MJ), and Harpin protein (HP) were applied by foliar aspersion on lettuce leaves while cultivated under hydroponic conditions. Application of elicitors was done at 15, 7, 5, 3 or 1 day before harvest. Green lettuce showed the highest increase in phytochemical content when elicitors (AA, SA, and HP) were applied on day 7 before harvest...
June 14, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28613108/a-novel-phylogroup-of-pseudomonas-cichorii-identified-following-an-unusual-disease-outbreak-on-tomato
#3
Sujan Timilsina, Heather Adkison, Anna Louise Testen, Eric A Newberry, Sally A Miller, Mathews Lal Paret, Gerald V Minsavage, Erica M Goss, Jeffrey B Jones, Gary E Vallad
Recently, in Central Florida tomato production fields, tomato foliage and fruit were observed with symptoms similar to bacterial speck. Fluorescent pseudomonads were consistently isolated and the strains were characterized by standard LOPAT tests, pathogenicity tests and genetic characterization using 16S rRNA sequences and multilocus sequence analysis of conserved housekeeping genes. LOPAT test results indicated the strains were likely P. cichorii. These strains were pathogenic on tomato and were also pathogenic on lettuce, the host for the type strain of P...
June 14, 2017: Phytopathology
https://www.readbyqxmd.com/read/28612389/biochar-potential-in-intensive-cultivation-of-capsicum-anuum-l-sweet-pepper-crop-yield-and-plant-protection
#4
Abhay Kumar, Yigal Elad, Ludmila Tsechansky, Vikas Abrol, Beni Lew, Rivka Offenbach, Ellen R Graber
BACKGROUND: The influence of various biochars on crop yield and disease resistance of Capsicum anuum L. (sweet pepper) under modern, high-input, intensive net house cultivation was tested over the course of 2011 to 2014 in the Arava desert region of Israel. A pot experiment with Lactuca sativa L. (lettuce) grown in the absence of fertilizer employed the 3-year old field trial soils to determine if biochar treatments contributed to soil intrinsic fertility. RESULTS: Biochar amendments resulted in a significant increase in number and weight of pepper fruits over three years...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28612342/effect-of-glycine-nitrogen-on-lettuce-growth-under-soilless-culture-a-metabolomics-approach-to-identify-the-main-changes-occurred-in-plant-primary-and-secondary-metabolism
#5
Xiao Yang, Lei Feng, Li Zhao, Xiaosong Liu, Danial Hassani, Danfeng Huang
BACKGROUND: Lettuce is a significant source of antioxidants and bioactive compounds. Nitrate is a cardinal fertilizer in horticulture and influences vegetable yield and quality; however, the negative effects of nitrate on the biosynthesis of flavonoids require further study. It is expected that using fertilizers containing organic N could promote the synthesis of health-promoting compounds. RESULTS: Lettuces were hydroponically cultured in media containing 9 mM nitrate or 9 mM glycine for 4 weeks...
June 14, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28609214/the-potyvirus-particle-recruits-the-plant-translation-initiation-factor-eif4e-by-means-of-the-vpg-covalently-linked-to-the-viral-rna
#6
Geneviève Tavert-Roudet, Agnès Anne, Amandine Barra, Arnaud Chovin, Christophe Demaille, Thierry Michon
The VPg of potyviruses is a protein covalently linked to the 5' end of viral RNA. It interacts with eIF4E, a component of the cellular translation initiation complex. It has been suggested that the 5' RNA linked VPg could mimic the cellular mRNA cap, promoting synthesis of viral proteins. We report here evidences for evidence for recruitment of the plant eIF4E by Lettuce mosaic virus (LMV, potyvirus) particles via the 5' RNA linked VPg. Analysis of the viral population was performed by ELISA-based tests, either with crude extracts of LMV-infected tissues or purified viral particles...
June 13, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28605983/phytotoxic-property-of-the-invasive-plant-tithonia-diversifolia-and-a-phytotoxic-substance
#7
Masahiko Suzuki, Arihiro Iwasaki, Kiyotake Suenaga, Hisashi Kato-Noguchi
Tithonia diversifolia (Hermsl.) A. Gray is a perennial invasive plant and spreads quickly in the invasive areas. The extracts of T. diversifolia were found to be toxic to several crop plant species such as rice, maize, sorghum, lettuce and cowpea, and several putative allelopathic substances were identified. However, there is limited information available for the effects of T. diversifolia on wild plants including weed plant species. We investigated the allelopathic potential of T. diversifolia extracts on weed plants, and searched for phytotoxic substances with allelopathic activity...
June 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28604176/irrigation-water-sources-and-time-intervals-as-variables-on-the-presence-of-campylobacter-spp-and-listeria-monocytogenes-on-romaine-lettuce-grown-in-muck-soil
#8
Evelyne Guévremont, Lisyanne Lamoureux, Mylène Généreux, Caroline Côté
Irrigation water has been identified as a possible source of vegetable contamination by foodborne pathogens. Risk management for pathogens such as Campylobacter spp. and Listeria monocytogenes in fields can be influenced by the source of the irrigation water and the time interval between last irrigation and harvest. Plots of romaine lettuce were irrigated with manure-contaminated water or aerated pond water 21, 7, or 3 days prior to harvesting, and water and muck soil samples were collected at each irrigation treatment...
June 12, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28594569/presence-of-human-pathogens-in-produce-from-retail-markets-in-northern-germany
#9
Gregor Fiedler, Jan Kabisch, Christina Böhnlein, Melanie Huch, Biserka Becker, Gyu-Sung Cho, Charles M A P Franz
Two hundred fresh produce samples (cucumber, carrots, herbs, leaf lettuce, and ready-to-eat mixed salad leaves) were obtained from retail in northern Germany in 2015. These were investigated for microbial contamination and the presence of foodborne pathogens, including Listeria monocytogenes, Salmonella serovars, presumptive Bacillus (B.) cereus, and Shiga toxin-producing Escherichia coli using culture-dependent (enrichment, plating on selective media) and -independent (real-time polymerase chain reaction [PCR]) techniques...
June 8, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28589271/surveillance-of-pesticide-residues-in-fruits-and-vegetables-from-accra-metropolis-markets-ghana-2010-2012-a-case-study-in-sub-saharan-africa
#10
Paul Osei Fosu, Augustine Donkor, Cephas Ziwu, Brajesh Dubey, Robert Kingsford-Adaboh, Isaac Asante, Stephen Nyarko, Rose Tawiah, Noble Nazzah
Monitoring of pesticide residues in food commodities of plant origin is part of the regular controls on food to safeguard consumer's health. This study reports for the first time in Ghana a 3-year (2010-2012) monitoring of pesticide contamination of fruits and vegetables and their health implications. A total of 3483 samples were purchased in notable markets within Accra Metropolis and analysed for pesticide residues, employing the modified quick, easy, cheap, effective, rugged and safe analytical procedure...
June 6, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28588603/short-term-effect-of-salt-shock-on-ethylene-and-polyamines-depends-on-plant-salt-sensitivity
#11
Pedro J Zapata, María Serrano, Manuel F García-Legaz, M T Pretel, M A Botella
In the present manuscript the short term effect (3-24 h) of a saline shock (NaCl 100 mM) on fresh weight, water content, respiration rate, ethylene production and Na(+), Cl(-), ACC and polyamine concentration was studied in four plant species with different salt sensitivity, pepper, lettuce, spinach, and beetroot. Higher reduction in fresh weight and water content as a consequence of saline shock was found in pepper and lettuce plants than in spinach and beetroot, the latter behaving as more salinity tolerant...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28585369/chemical-composition-and-antibacterial-antifungal-allelopathic-and-acetylcholinesterase-inhibitory-activities-of-cassumunar-ginger
#12
Ram S Verma, Neeta Joshi, Rajendra C Padalia, Ved R Singh, Prakash Goswami, Sajendra K Verma, Hina Iqbal, Debabrata Chanda, Rajesh K Verma, Mahendra P Darokar, Amit Chauhan, Manish K Kandwal
BACKGROUND: Zingiber montanum (J.Koenig) Link ex A.Dietr. (Zingiberaceae), commonly known as cassumunar-ginger, is a folk remedy for the treatments of inflammations, sprains, rheumatism and asthma. The aim of this study was to assess the chemical composition, and antibacterial, antifungal, allelopathic and acetylcholinesterase inhibitory activities of the essential oil of Z. montanum originated from India. RESULTS: The hydrodistilled essential oil of Z. montanum rhizome was analyzed using GC-FID and GC-MS...
June 6, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28578228/keeping-it-cool-survival-of-giardia-cysts-and-cryptosporidium-oocysts-on-lettuce-leaves
#13
Kjersti Selstad Utaaker, Eystein Skjerve, Lucy J Robertson
Fresh produce has been recognized as a vehicle for transmission of protozoan parasites for many years, and there are numerous publications regarding their occurrence on such foodstuffs, indicating their potential importance as foodborne parasites. Nevertheless, few studies have been published regarding the effectiveness of this transmission route, and whether contamination is likely to result in transmission. The purpose of this study was to assess the viability of Cryptosporidium oocysts and Giardia cysts, two protozoa associated with both waterborne and foodborne transmission, by spiking fresh produce (lettuce leaves) with viable transmission stages and determining changes in viability...
May 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28576742/draft-genome-sequence-of-a-ctx-m-15-producing-endophytic-klebsiella-pneumoniae-st198-isolate-from-commercial-lettuce
#14
Ralf Lopes, Louise T Cerdeira, Miriam R Fernandes, Paula J Pérez-Chaparro, John A McCulloch, Nilton Lincopan
CTX-M-15-producing Klebsiella pneumoniae is one of the leading causes of hospital-acquired infections globally. In this study, we present the draft genome sequence of the first CTX-M-15-producing endophytic K. pneumoniae strain, belonging to sequence type 198 (ST198) and isolated from commercial lettuce, which can be used as a reference sequence for comparative analysis with clinical and environmental strains of K. pneumoniae.
May 30, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28576376/impact-of-solar-radiation-exposure-on-phyllosphere-bacterial-community-of-red-pigmented-baby-leaf-lettuce
#15
Pilar Truchado, M Isabel Gil, Patricia Reboleiro, Belén Rodelas, Ana Allende
Solar radiation has been identified as a stress factor affecting phyllosphere associated bacteria colonization and survival during primary production. In the present study, the impact of different solar radiation doses on the phyllosphere microbiota of red-pigmented baby leaf lettuce cultivated in open field under commercial conditions was evaluated. Four weeks before harvest, the growing field was divided into four plots; each one was consecutively covered with one-week-interval with a light-excluding plastic to reduce the sunlight exposure...
September 2017: Food Microbiology
https://www.readbyqxmd.com/read/28576363/effect-of-green-tea-extract-on-enteric-viruses-and-its-application-as-natural-sanitizer
#16
W Randazzo, I Falcó, R Aznar, G Sánchez
In this work, the effect of green tea extract (GTE) was assessed against murine norovirus (MNV) and hepatitis A virus (HAV) at different temperatures, exposure times and pH conditions. Initially, GTE at 0.5 and 5 mg/ml were individually mixed with each virus at 5 log TCID50/ml and incubated 2 h at 37 °C at different pHs (from 5.5 to 8.5). GTE affected both viruses depending on pH with higher reductions observed in alkaline conditions. Secondly, different concentrations of GTE (0.5 and 5 mg/ml) were mixed with viral suspensions and incubated for 2 or 16 h at 4, 25 and 37 °C at pH 7...
September 2017: Food Microbiology
https://www.readbyqxmd.com/read/28575911/rapid-transient-production-of-a-monoclonal-antibody-neutralizing-the-porcine-epidemic-diarrhea-virus-pedv-in-nicotiana-benthamiana-and-lactuca-sativa
#17
Kaewta Rattanapisit, Anchalee Srijangwad, Taksina Chuanasa, Suchada Sukrong, Angkana Tantituvanont, Hugh S Mason, Dachrit Nilubol, Waranyoo Phoolcharoen
Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration, weight loss, and high mortality rate in neonatal piglets. Porcine epidemic diarrhea (PED) has been reported in Europe, America, and Asia including Thailand. The disease causes substantial losses to the swine industry in many countries. Presently, there is no effective PEDV vaccine available. In this study, we developed a plant-produced monoclonal antibody (mAb) 2C10 as a prophylactic candidate to prevent the PEDV infection...
June 2, 2017: Planta Medica
https://www.readbyqxmd.com/read/28553632/hydroquinone-a-novel-bioactive-compound-from-plant-derived-smoke-can-cue-seed-germination-of-lettuce
#18
Muhammad Kamran, Abdul L Khan, Liaqat Ali, Javid Hussain, Muhammad Waqas, Ahmed Al-Harrasi, Qari M Imran, Yoon-Ha Kim, Sang-Mo Kang, Byung-Wook Yun, In-Jung Lee
Plant-derived smoke has been known to play an important role in distribution and growth of vegetation. Using a proficiently designed furnace, we extracted smoke from the leaves of four plant viz. Helianthus annuus,Aloe vera,Ginkgo biloba, and Cymbopogon jwarancusa. Smoke dilutions obtained from these plants were obtained in different concentrations to identify potential lettuce growth promoting smoke solution. Results revealed that smoke obtained from G. biloba significantly enhanced the lettuce seed germination...
2017: Frontiers in Chemistry
https://www.readbyqxmd.com/read/28550806/lettuce-facing-microcystins-rich-irrigation-water-at-different-developmental-stages-effects-on-plant-performance-and-microcystins-bioaccumulation
#19
Efi Levizou, George Statiris, Theodoti Papadimitriou, Chrysi S Laspidou, Konstantinos Ar Kormas
This study investigated the microcystins (MCs)-rich irrigation water effect on lettuce of different developmental stages, i.e. during a two months period, covering the whole period from seed germination to harvest at marketable size of the plant. We followed four lettuce plant groups receiving MCs-rich water (1.81μgl(-1) of dissolved MCs), originating from the Karla Reservoir, central Greece: 1) from seeds, 2) the cotyledon, 3) two true leaves and 4) four true leaves stages, all of which were compared to control plants that received tap water...
May 24, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28542913/reduction-of-escherichia-coli-o157-h7-in-biofilms-using-bacteriophage-bpeco-19
#20
Mohammad Sadekuzzaman, Sungdae Yang, Md Furkanur Rahaman Mizan, Sang-Do Ha
Biofilm formation is a growing concern in the food industry. Escherichia coli O157:H7 is one of the most important foodborne pathogens that can persists in food and food-related environments and subsequently produce biofilms. The efficacy of bacteriophage BPECO 19 was evaluated against three E. coli O157:H7 strains in biofilms. Biofilms of the three E. coli O157:H7 strains were grown on abiotic (stainless steel, rubber, and minimum biofilm eradication concentration [MBEC(TM) ] device) and biotic (lettuce) surfaces at different temperatures...
June 2017: Journal of Food Science
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