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Candida albicans IgM

Luciano Polonelli, Tecla Ciociola, Lisa Elviri, Pier Paolo Zanello, Laura Giovati, Denise C Arruda, Julián E Muñoz, Renato A Mortara, Giulia Morace, Elisa Borghi, Serena Galati, Oriano Marin, Claudio Casoli, Elisabetta Pilotti, Paola Ronzi, Luiz R Travassos, Walter Magliani, Stefania Conti
A phosphorylated peptide, named K40H, derived from the constant region of IgMs was detected in human serum by liquid chromatography coupled to high-resolution mass spectrometry. Synthetic K40H proved to exert a potent in vitro activity against fungal pathogens, and to inhibit HIV-1 replication in vitro and ex vivo. It also showed a therapeutic effect against an experimental infection by Candida albicans in the invertebrate model Galleria mellonella. K40H represents the proof of concept of the innate role that naturally occurring antibody fragments may exert against infectious agents, shedding a new light upon the posthumous role of antibodies and opening a new scenario on the multifaceted functionality of humoral immunity...
October 11, 2016: Scientific Reports
Li Wang, Lan Yan, Xing Xing Li, Guo Tong Xu, Mao Mao An, Yuan Ying Jiang
Candida albicans is the most common cause of invasive fungal infections in humans. The C. albicans cell wall proteins play an important role in crucial host-fungus interactions and might be ideal vaccine targets to induce protective immune response in host. Meanwhile, protein that is specific to C. albicans is also an ideal target of vaccine. In this study, 11 proteins involving cell wall biosynthesis, yeast-to-hypha formation, or specific to C. albicans were chosen and were successfully cloned, purified and verified...
2015: Biological & Pharmaceutical Bulletin
Ghaffari Javad, Mehdi Taheri Sarvtin, Mohammad Taghi Hedayati, Zohreh Hajheydari, Jamshid Yazdani, Tahereh Shokohi
The aim of this study was to assess the candidal colonization and specific humoral responses against Candida albicans in patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagar Candida medium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodies against C. albicans in sera of participants...
2015: BioMed Research International
Cevahir Aydın, Haluk Ataoğlu
Candida albicans is a polymorphic fungus that may be observed as both commensal and opportunistic pathogen in humans. As one of the major components of Candida cell wall structure, mannan plays an important role in the fungus-host cell interaction and in virulence. The ability to switch from yeast to hypha form of microorganism is crutial in the development of C.albicans infections. Hyphal form has different antigenic properties compared to yeast form and structural changes occur in the yeast cell wall during transition from yeast to hypha form...
January 2015: Mikrobiyoloji Bülteni
Ema Paulovičová, Helena Bujdáková, Jarmila Chupáčová, Lucia Paulovičová, Pavol Kertys, Martin Hrubiško
In vitro evaluation of specific anti-Candida albicans sera antibodies based on synthetically prepared complement receptor 3-related protein (CR3-RP) mimicking the structure of native complement receptor 3 in a cohort of 72 patients with atopy and recurrent Candida vulvovaginitis (RVC) revealed effective humoral response against Candida CR3-RP. The most significant have been IgM and IgA isotype antibodies (33 and 47% positive cases, respectively). The quantitative evaluation of anti-CR3RP isotype antibodies was confronted with results of commercial ELISA anti-C...
March 2015: FEMS Yeast Research
M Suchankova, E Paulovicova, L Paulovicova, I Majer, E Tedlova, H Novosadova, E Tibenska, M Tedla, M Bucova
The recent studies suggest a role of fungi in development of sarcoidosis. Moreover, the immune response in sarcoidosis and fungal infection shows a striking similarity. We formulated a hypothesis of the possible increase in antifungal antibodies in bronchoalveolar lavage fluid (BALF) and serum in pulmonary sarcoidosis. BALF and serum levels of IgG-, IgM- and IgA-specific antibodies against the cell wall β-D-glucan and mannan of Candida albicans and Saccharomyces cerevisiae were tested in 47 patients (29 pulmonary sarcoidosis patients and 18 patients with other interstitial lung diseases (ILD - control group)) and 170 healthy controls...
April 2015: Scandinavian Journal of Immunology
Mehdi Taheri Sarvtin, Tahereh Shokohi, Zohreh Hajheydari, Jamshid Yazdani, Mohammad T Hedayati
BACKGROUND: Psoriasis is an inflammatory skin disease that can considerably affect a patient's quality of life. Environmental and genetic factors, as well as superantigens and toxins from Candida species, may play various roles in the exacerbation and persistence of psoriasis. In the present study, we evaluated candidal colonization and specific humoral responses against Candida albicans in patients with psoriasis. METHODS: A total of 100 patients with psoriasis vulgaris and 50 healthy control individuals were enrolled in the study...
December 2014: International Journal of Dermatology
Maya J Nurgeldiyeva, Bayram G Hojakuliyev, Merdan B Muhammedov
PURPOSE: To study contents of atherosclerotic plaques for the presence of fungi of the genus Candida; and an analysis of some immunological and biochemical indices in patients with acute coronary syndrome (ACS) that are positive for Candida albicans. MATERIALS AND METHODS: To test for the presence of fungi in an atherosclerotic plaque, we used a method developed by us (patent NO 531, a priority from 6/28/2010). A total of 47 atherosclerotic plaques were obtained during 20 autopsies...
2014: International Journal of Clinical and Experimental Medicine
Luciano Polonelli, Concetta Beninati, Giuseppe Teti, Franco Felici, Tecla Ciociola, Laura Giovati, Martina Sperindè, Carla Lo Passo, Ida Pernice, Maria Domina, Milena Arigò, Salvatore Papasergi, Giuseppe Mancuso, Stefania Conti, Walter Magliani
A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR...
2014: PloS One
Genna E Davies, Christopher R Thornton
The fungal genus Trichosporon contains emerging opportunistic pathogens of humans, and is the third most commonly isolated non-candidal yeast from humans. Trichosporon asahii and T. asteroides are the most important species causing disseminated disease in immunocompromised patients, while inhalation of T. asahii spores is the most important cause of summer-type hypersensitivity pneumonitis in healthy individuals. Trichosporonosis is misdiagnosed as candidiasis or cryptococcosis due to a lack of awareness and the ambiguity of diagnostic tests for these pathogens...
2014: PloS One
Rong Tian, Meng Fu, Zhuo Zhang, Jing Ren, Jingang An, Yufeng Liu, Wei Li
B-1 cells are innate-like cells that play important roles in host defense against infection. However, the function of B-1 cells in fungi infection remains unclear. Previously we produced IgH transgenic mice TgVH3B4 with VH derived from a natural antibody 3B4 that can identify C. albicans, and found that TgVH3B4 mice were resistant to intraperitoneal (i. p.) and intravenous C. albicans infection. Most of the peritoneal cavity (PEC) B-1 cells in TgVH3B4 mice express transgenic BCR that binds C. albicans. In the present study, we explored the response of B-1 cells to C...
2013: PloS One
L Paulovičová, E Paulovičová, A A Karelin, Y E Tsvetkov, N E Nifantiev, S Bystrický
Several studies have established the potential efficacy of humoral immunity, primarily mannan-specific antibodies, in host protection against major fungal pathogen Candida albicans. In this study, we analysed humoral immune response induced by immunization with BSA-based conjugates bearing synthetic α-1,6-branched oligomannosides (pentamannosides (M5) or hexamannosides (M6)) mimicking antigenic sequences of Candida cell wall mannan. We analysed the ability of antibodies prepared by immunization to recognize relevant antigenic determinants in mannan polysaccharide structure and in C...
June 2013: Scandinavian Journal of Immunology
Luciano Polonelli, Tecla Ciociola, Walter Magliani, Pier Paolo Zanello, Tiziana D'Adda, Serena Galati, Flavia De Bernardis, Silvia Arancia, Elena Gabrielli, Eva Pericolini, Anna Vecchiarelli, Denise C Arruda, Marcia R Pinto, Luiz R Travassos, Thelma A Pertinhez, Alberto Spisni, Stefania Conti
Synthetic peptides with sequences identical to fragments of the constant region of different classes (IgG, IgM, IgA) of antibodies (Fc-peptides) exerted a fungicidal activity in vitro against pathogenic yeasts, such as Candida albicans, Candida glabrata, Cryptococcus neoformans, and Malassezia furfur, including caspofungin and triazole resistant strains. Alanine-substituted derivatives of fungicidal Fc-peptides, tested to evaluate the critical role of each residue, displayed unaltered, increased or decreased candidacidal activity in vitro...
2012: PloS One
Otavio Cabral-Marques, Lena-Friederike Schimke, Paulo Vítor Soeiro Pereira, Angela Falcai, João Bosco de Oliveira, Mary J Hackett, Paolo Ruggero Errante, Cristina Worm Weber, Janaíra Fernandes Ferreira, Gisele Kuntze, Nelson Augusto Rosário-Filho, Hans D Ochs, Troy R Torgerson, Beatriz Tavares Costa Carvalho, Antonio Condino-Neto
CD40 ligand (CD40L) deficiency or X-linked hyper-IgM syndrome (X-HIGM) is a well-described primary immunodeficiency in which Pneumocystis jiroveci pneumonia is a common clinical feature. We have identified an unusual high incidence of fungal infections and other not yet described infections in a cohort of 11 X-HIGM patients from nine unrelated Brazilian families. Among these, we describe the first case of paracoccidioidomycosis (PCM) in X-HIGM. The molecular genetic analysis of CD40L was performed by gene sequencing and evaluation of CD40L protein expression...
April 2012: Journal of Clinical Immunology
Craig Boulton, Karin Meiser, Olivier J David, Robert Schmouder
Fingolimod, a first-in-class oral sphingosine 1-phosphate receptor (S1PR) modulator, is approved in many countries for relapsing-remitting multiple sclerosis, at a once-daily 0.5-mg dose. A reduction in peripheral lymphocyte count is an expected consequence of the fingolimod mechanism of S1PR modulation. The authors investigated if this pharmacodynamic effect impacts humoral and cellular immunogenicity. In this double-blind, parallel-group, 4-week study, 72 healthy volunteers were randomized to steady state, fingolimod 0...
December 2012: Journal of Clinical Pharmacology
Otavio Cabral-Marques, Christina Arslanian, Rodrigo Nalio Ramos, Mariana Morato, Lenafriederike Schimke, Paulo Vitor Soeiro Pereira, Sonia Jancar, Janaíra Fernandes Ferreira, Cristina Worm Weber, Gisele Kuntze, Nelson Augusto Rosario-Filho, Beatriz Tavares Costa Carvalho, Patricia Cruz Bergami-Santos, Mary J Hackett, Hans D Ochs, Troy R Torgerson, Jose Alexandre Marzagão Barbuto, Antonio Condino-Neto
BACKGROUND: Patients with X-linked hyper-IgM syndrome (X-HIGM) due to CD40 ligand (CD40L) mutations are susceptible to fungal pathogens; however, the underlying susceptibility mechanisms remain poorly understood. OBJECTIVE: To determine whether monocyte-derived dendritic cells (DCs) from patients with X-HIGM exhibit normal responses to fungal pathogens. METHODS: DCs from patients and controls were evaluated for the expression of costimulatory (CD80 and CD86) and MHC class II molecules and for their ability to produce IL-12 and IL-10 in response to Candida albicans and Paracoccidioides brasiliensis...
March 2012: Journal of Allergy and Clinical Immunology
R A Bryan, A J Guimaraes, S Hopcraft, Z Jiang, K Bonilla, A Morgenstern, F Bruchertseifer, M Del Poeta, A Torosantucci, A Cassone, J D Nosanchuk, A Casadevall, E Dadachova
BACKGROUND: Previously, we demonstrated the ability of radiolabeled antibodies recognizing the cryptococcal polysaccharide capsule to kill Cryptococcus neoformans both in vitro and in infected mice. This approach, known as radioimmunotherapy (RIT), uses the exquisite ability of antibodies to bind antigens to deliver microbicidal radiation. To create RIT reagents which would be efficacious against all major medically important fungi, we have selected monoclonal antibodies (mAbs) to common surface fungal antigens such as heat shock protein 60 (HSP60), which is found on the surface of diverse fungi; beta (1,3)-glucan, which is a major constituent of fungal cell walls; ceramide which is found at the cell surface, and melanin, a polymer present in the fungal cell wall...
June 2012: Mycopathologia
Armando Heriazon, Korinne Hamilton, Jennifer Huffman, Bruce N Wilkie, William Sears, Margaret Quinton, Bonnie A Mallard
Infectious diseases are detrimental to the health and economy of the livestock industry. Observations of cattle resistant to natural infections have implied the feasibility of breeding livestock for disease resistance. Studies of pigs selected for antibody (AMIR)- and cell (CMIR)-mediated immune responses have demonstrated increased immune responsiveness suggesting enhanced protection by both type 2 and type 1 responses, respectively. Additionally, natural or artificial infections of cattle suggest that the production of particular immunoglobulin (Ig) M, IgG1 and IgG2 isotypes are important for protecting against pathogens...
December 15, 2011: Veterinary Immunology and Immunopathology
Olivier Denis, Anne Van Cauwenberge, Greta Treutens, Bouazza Es Saadi, Françoise Symoens, Nathalie Popovic, Kris Huygen
In this study, three different rat hybridoma cell lines secreting monoclonal antibodies (mAbs) recognizing the spores from Alternaria alternata, a plant pathogenic fungus, contaminant of food products and important cause of both allergic rhinitis and asthma, have been characterized. These three mAbs are all of IgM isotype. Two antibodies, A1 and F10, were cross-reactive antibodies recognizing spores from Alternaria, Cladosporium, Penicillium, Aspergillus and Stachybotrys genera, but not the yeasts Saccharomyces cerevisiae or Candida albicans...
March 2012: Mycopathologia
Hong Xin, Jim E Cutler
Previously we showed that antibodies specific for the glycan β-1,2-mannotriose [β-(Man)(3)] on the cell surface of Candida albicans protect mice against disseminated candidiasis (H. Xin, S. Dziadek, D. R. Bundle, and J. E. Cutler, Proc. Natl. Acad. Sci. U. S. A. 105:13526-13531, 2008). Furthermore, six 14-mer peptides that are within the N-terminal portion of C. albicans wall proteins were conjugated to the glycan in an attempt to create immunogenic glycopeptide conjugates. By a dendritic cell (DC)-based immunization approach, all were immunogenic and three of the six conjugates induced a high degree of protection in mice...
October 2011: Clinical and Vaccine Immunology: CVI
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