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Candida albicans IgG

Zheng-Xin He, Lan-Chun Shi, Xiang-Yang Ran, Wei Li, Xian-Ling Wang, Fu-Kun Wang
Early and accurate diagnosis of invasive candidiasis (IC) is very important. In this study, a lateral flow immunoassay (LFIA) was developed to detect antibody against Candida albicans enolase (Eno). Colloidal gold particle labeled mouse anti human IgG (1.0 mg/L) was used as the detector reagent. Recombinant enolase (rEno, 1.0 mg/L) and goat anti IgG (1.0 mg/L) were immobilized in test and control lines, respectively, of a nitrocellulose membrane, acting as the capture reagents. The LFIA was used to detect anti Eno in 38 sera from clinically proven IC patients, as well as in 50 healthy control subjects...
2016: Frontiers in Microbiology
Guochao Liao, Zhifang Zhou, Jun Liao, Luning Zu, Qiuye Wu, Zhongwu Guo
With the rapid growth in fungal infections and drug-resistant fungal strains, antifungal vaccines have become an especially attractive strategy to tackle this important health problem. β-Glucans, a class of extracellular carbohydrate antigens abundantly and consistently expressed on fungal cell surfaces, are intriguing epitopes for antifungal vaccine development. β-Glucans have a conserved β-1,3-glucan backbone with sporadic β-1,3- or β-1,6-linked short glucans as branches at the 6-O-positions, and the branches may play a critical role in their immunologic functions...
February 12, 2016: ACS Infectious Diseases
Blanca Huertas, Daniel Prieto, Aida Pitarch, Concha Gil, Jesus Pla, Rosalia Diez-Orejas
Candida albicans is a commensal microorganism in the oral cavity, gastrointestinal and urogenital tracts of most individuals that acts like as an opportunistic pathogen when the host immune responses is reduced. Here, we settled down different immunocompetent murine models, to analyze the antibody responses to the C. albicans proteome during commensalism, commensalism followed by infection, and infection (C, C+I and I models, respectively). Serum anti-C. albicans IgG antibody levels were higher in colonized mice than in infected mice...
August 18, 2016: Journal of Proteome Research
Emily G Severance, Kristin L Gressitt, Catherine R Stallings, Emily Katsafanas, Lucy A Schweinfurth, Christina L Savage, Maria B Adamos, Kevin M Sweeney, Andrea E Origoni, Sunil Khushalani, F Markus Leweke, Faith B Dickerson, Robert H Yolken
Immune aberrations in schizophrenia and bipolar disorder have led to the hypotheses that infectious agents or corresponding immune responses might contribute to psychiatric etiopathogeneses. We investigated case-control differences in exposure to the opportunistic fungal pathogen, Candida albicans, and examined associations with cognition, medication, lifestyle, and somatic conditions. We quantified C. albicans IgG antibodies in two cohorts totaling 947 individuals and evaluated odds ratios (OR) of exposure with psychiatric disorder using multivariate regressions...
2016: NPJ Schizophrenia
Catarina Carneiro, Alexandra Correia, Tânia Lima, Manuel Vilanova, Célia Pais, Andreia C Gomes, M Elisabete C D Real Oliveira, Paula Sampaio
UNLABELLED: We evaluated the potential of a liposomal antigen delivery system (ADS) containing Candida albicans cell wall surface proteins (CWSP) in mediating protection against systemic candidiasis. Treatment of bone-marrow-derived dendritic cells with CWSP-loaded dioctadecyldimethylammonium bromide:monoolein (DODAB:MO) liposomes enhanced and prolonged their activation comparatively to free antigen, indicating that liposome-entrapped CWSP were released more sustainable. Therefore, we immunized mice with CWSP either in a free form or loaded into two different DODAB:MO liposome formulations, respectively designated as ADS1 and ADS2, prior to intravenous C...
July 15, 2016: Acta Biomaterialia
Fan Yang, Chunyu Xiao, Jing Qu, Guiyun Wang
Structure and immunologic enhancement of low molecular weight polysaccharide (LMW-ASP) isolated from the root of Astragalus membranaceus (Fisch) Bge. Were detected in recombinant protein vaccine. Structure analysis of LMW-ASP revealed that LMW-ASP (Mw=5.6kDa) was an acid heteropolysaccharide, which consisted of Glc, Gal, Ara, Xyl and GalA in ratio of 10.0:1.3:1.7:1.0:0.9. Recombinant protein (rP-HSP90C) contained epitope C (LKVIRK) from heat shock protein 90 (HSP90) of Candida albicans was used as a vaccine...
July 10, 2016: Carbohydrate Polymers
Tecla Ciociola, Thelma A Pertinhez, Laura Giovati, Martina Sperindè, Walter Magliani, Elena Ferrari, Rita Gatti, Tiziana D'Adda, Alberto Spisni, Stefania Conti, Luciano Polonelli
Synthetic peptides encompassing sequences related to the complementarity-determining regions of antibodies or derived from their constant region (Fc peptides) were proven to exert differential antimicrobial, antiviral, antitumor, and/or immunomodulatory activitiesin vitroand/orin vivo, regardless of the specificity and isotype of the parental antibody. Alanine substitution derivatives of these peptides exhibited unaltered, increased, or decreased candidacidal activitiesin vitro The bioactive IgG-derived Fc N10K peptide (NQVSLTCLVK) spontaneously self-assembles, a feature previously recognized as relevant for the therapeutic activity of another antibody-derived peptide...
April 2016: Antimicrobial Agents and Chemotherapy
Renata Stawerska, Elzbieta Czkwianianc, Agnieszka Matusiak, Joanna Smyczyńska, Maciej Hilczer, Magdalena Chmiela, Andrzej Lewiński
OBJECTIVES: Many of peptides synthesized in gastrointestinal tract (GI) and adipose tissues, regulate growth and food intake. The GI microflora is an antigenic source. Based on the molecular mimicry hypothesis, intestinal microbe-derived antigens may trigger the production of autoantibodies cross-reacting with some neuropeptides. DESIGN: The aim of the study was to assess whether in idiopathic short stature (ISS) children with Candida albicans (C.albicans) colonisation and/or Helicobacter pylori (H...
2015: Neuro Endocrinology Letters
Li Wang, Lan Yan, Xing Xing Li, Guo Tong Xu, Mao Mao An, Yuan Ying Jiang
Candida albicans is the most common cause of invasive fungal infections in humans. The C. albicans cell wall proteins play an important role in crucial host-fungus interactions and might be ideal vaccine targets to induce protective immune response in host. Meanwhile, protein that is specific to C. albicans is also an ideal target of vaccine. In this study, 11 proteins involving cell wall biosynthesis, yeast-to-hypha formation, or specific to C. albicans were chosen and were successfully cloned, purified and verified...
2015: Biological & Pharmaceutical Bulletin
Paulo S R Coelho, Hogune Im, Karl V Clemons, Michael P Snyder, David A Stevens
We profiled the global immunoglobulin response against fungal infection by using yeast protein microarrays. Groups of CD-1 mice were infected systemically with human fungal pathogens (Coccidioides posadasii, Candida albicans, or Paracoccidioides brasiliensis) or inoculated with PBS as a control. Another group was inoculated with heat-killed yeast (HKY) of Saccharomyces cerevisiae. After 30 days, serum from mice in the groups were collected and used to probe S. cerevisiae protein microarrays containing 4800 full-length glutathione S-transferase (GST)-fusion proteins...
September 4, 2015: Journal of Proteome Research
Yoshifumi Okochi, Yasuaki Aratani, Hibret A Adissu, Nana Miyawaki, Mari Sasaki, Kazuo Suzuki, Yasushi Okamura
Neutrophil granule exocytosis is crucial for host defense and inflammation. Neutrophils contain 4 types of granules, the exocytotic release of which is differentially regulated. This exocytosis is known to be driven by diverse mediators, including calcium and nucleotides, but the precise molecular mechanism remains largely unknown. We show in the present study that voltage-gated proton (Hv) channels are necessary for the proper release of azurophilic granules in neutrophils. On activation of NADPH oxidase by PMA and IgG, neutrophils derived from Hvcn1 gene knockout mouse exhibited greater secretion of MPO and elastase than WT cells...
January 2016: Journal of Leukocyte Biology
Andrew C Issekutz, Derek Rowter, Sylvia Miescher, Fabian Käsermann
IVIG modulates T cell activation in vitro and inflammatory-autoimmune conditions in vivo. Sialylation of IgG, Fc receptor interactions, modulation of monocyte/macrophage/B cell functions have been implicated in IVIG effects. Subcutaneous IgG (SCIG) therapy is increasingly used for IgG replacement but whether these preparations share the effects of IVIG on T cell modulation is not documented. We compared the potency of SCIG-Hizentra™ (20% IgG preparation) with IVIG-Privigen® (10% IgG) for T cell inhibition, and assessed the involvement of IgG sialylation, monocytes and B cells in this process...
October 2015: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Ghaffari Javad, Mehdi Taheri Sarvtin, Mohammad Taghi Hedayati, Zohreh Hajheydari, Jamshid Yazdani, Tahereh Shokohi
The aim of this study was to assess the candidal colonization and specific humoral responses against Candida albicans in patients with atopic dermatitis. One hundred patients with atopic dermatitis and 50 healthy individuals were enrolled in the study. Skin and oral specimens from all participants were cultured on CHROMagar Candida medium. Isolated yeasts were identified by using the sequence of the D1/D2 domain of the 26S rRNA gene. ELISA was used for detection of IgM, IgA, and IgG antibodies against C. albicans in sera of participants...
2015: BioMed Research International
Yicun Wang, Zhigang Ju, Binrui Cao, Xiang Gao, Ye Zhu, Penghe Qiu, Hong Xu, Pengtao Pan, Huizheng Bao, Li Wang, Chuanbin Mao
Candida albicans (C. albicans) infection causes high mortality rates within cancer patients. Due to the low sensitivity of the current diagnosis systems, a new sensitive detection method is needed for its diagnosis. Toward this end, here we exploited the capability of genetically displaying two functional peptides, one responsible for recognizing the biomarker for the infection (antisecreted aspartyl proteinase 2 IgG antibody) in the sera of cancer patients and another for binding magnetic nanoparticles (MNPs), on a single filamentous fd phage, a human-safe bacteria-specific virus...
2015: ACS Nano
Seiji Shibasaki, Miki Karasaki, Senji Tafuku, Wataru Aoki, Tomomitsu Sewaki, Mitsuyoshi Ueda
Candidiasis is a common fungal infection that is prevalent in immunocompromised individuals. In this study, an oral vaccine against Candida albicans was developed by using the molecular display approach. Enolase 1 protein (Eno1p) of C. albicans was expressed on the Lactobacillus casei cell surface by using poly-gamma-glutamic acid synthetase complex A from Bacillus subtilis as an anchoring protein. The Eno1p-displaying L. casei cells were used to immunize mice, which were later challenged with a lethal dose of C...
September 2014: Scientia Pharmaceutica
Marion Corouge, Séverine Loridant, Chantal Fradin, Julia Salleron, Sébastien Damiens, Maria Dolores Moragues, Vianney Souplet, Thierry Jouault, Raymond Robert, Sylvain Dubucquoi, Boualem Sendid, Jean Fréderic Colombel, Daniel Poulain
OBJECTIVE: The protein Hwp1, expressed on the pathogenic phase of Candida albicans, presents sequence analogy with the gluten protein gliadin and is also a substrate for transglutaminase. This had led to the suggestion that C. albicans infection (CI) may be a triggering factor for Celiac disease (CeD) onset. We investigated cross-immune reactivity between CeD and CI. METHODS: Serum IgG levels against recombinant Hwp1 and serological markers of CeD were measured in 87 CeD patients, 41 CI patients, and 98 healthy controls (HC)...
2015: PloS One
Eva Machová, Jana Korcová, Alžbeta Cížová, Slavomír Bystrický
A new approach to obtain broadly cross-reactive antisera against important yeast pathogens by intensive hyperimmunization with polysaccharide-protein conjugates is described here. Surface mannan of Candida albicans and capsular galactoglucoxylomannan of Cryptococcus laurentii were isolated and chemically linked to human serum albumin. Antisera elicited by a 7-week vigorous immunization of rabbits with the conjugates showed effective cross-reactive growth inhibition of different representatives of Candida spp...
July 2015: Journal of Microbiology and Biotechnology
Guochao Liao, Zhifang Zhou, Srinivas Burgula, Jun Liao, Cheng Yuan, Qiuye Wu, Zhongwu Guo
Antifungal vaccines have recently engendered considerable excitement for counteracting the resurgence of fungal infections. In this context, β-glucan, which is abundantly expressed on all fungal cell surfaces, functionally necessary for fungi, and immunologically active, is an attractive target antigen. Aiming at the development of effective antifungal vaccines based on β-glucan, a series of its oligosaccharide derivatives was designed, synthesized, and coupled with a carrier protein, keyhole limpet hemocyanin (KLH), to form new semisynthetic glycoconjugate vaccines...
March 18, 2015: Bioconjugate Chemistry
M Suchankova, E Paulovicova, L Paulovicova, I Majer, E Tedlova, H Novosadova, E Tibenska, M Tedla, M Bucova
The recent studies suggest a role of fungi in development of sarcoidosis. Moreover, the immune response in sarcoidosis and fungal infection shows a striking similarity. We formulated a hypothesis of the possible increase in antifungal antibodies in bronchoalveolar lavage fluid (BALF) and serum in pulmonary sarcoidosis. BALF and serum levels of IgG-, IgM- and IgA-specific antibodies against the cell wall β-D-glucan and mannan of Candida albicans and Saccharomyces cerevisiae were tested in 47 patients (29 pulmonary sarcoidosis patients and 18 patients with other interstitial lung diseases (ILD - control group)) and 170 healthy controls...
April 2015: Scandinavian Journal of Immunology
Susan Stockton, Karen Breshears, David McA Baker
BACKGROUND: Optimal human function and performance through diet strategies are critical for everyone but especially for those involved in collegiate or professional athletics. Currently, individualized medicine (IM) is emerging as a more efficacious approach to health with emphasis on personalized diet strategies for the public and is common practice for elite athletes. One method for directing patient-specific foods in the diet, while concomitantly impacting physical performance, may be via IgG food sensitivity and Candida albicans analysis from dried blood spot (DBS) collections...
November 2014: Global Advances in Health and Medicine: Improving Healthcare Outcomes Worldwide
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