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Himanshi Rathore, Abhishek Sharma, Shalinee Prasad, Satyawati Sharma
Calocybe indica was cultivated on wheat straw enriched with various concentrations of sodium selenite (Na2 SeO3 ), (2.5-40 μg/ml). The content of selenium (Se) in fruit bodies increased linearly by increasing the amount of Na2 SeO3 , although the biomass yield inhibited above 5 μg/ml. The fruit bodies accumulated inorganic Se into organic forms by integrating the Se into proteins (56%-68%), polysaccharides (22%-29%), and nucleic acids (1.4%-2.7%). Maximum protein content (25.31 g/100 g) was observed in fruit bodies harvested from the substrate enriched with 10 μg/ml Se...
May 15, 2018: Journal of Bioscience and Bioengineering
Esti Wahyu Widowati, Simone Bamberg-Lemper, Walter Becker
OBJECTIVE: Dual specificity tyrosine phosphorylation-regulated kinases (DYRK) contain a characteristic sequence motif (DYRK homology box, DH box) that is located N-terminal of the catalytic domain and supports the autophosphorylation of a conserved tyrosine during maturation of the catalytic domain. Two missense mutations in the DH box of human DYRK1B were recently identified as causative of a rare familiar form of metabolic syndrome. We have recently shown that these amino acid exchanges impair maturation of the kinase domain...
May 15, 2018: BMC Research Notes
Selvaraj Muthukrishnan, Munish Puri
OBJECTIVES: The arrival of free oxygen on the globe, aerobic life is becoming possible. However, it has become very clear that the oxygen binding proteins are widespread in the biosphere and are found in all groups of organisms, including prokaryotes, eukaryotes as well as in fungi, plants, and animals. The exponential growth and availability of fresh annotated protein sequences in the databases motivated us to develop an improved version of "Oxypred" for identifying oxygen-binding proteins...
May 11, 2018: BMC Research Notes
Rahman Md Moshikur, Chowdhury Md Raihan, Rie Wakabayashi, Yoshiro Tahara, Muhammad Moniruzzaman, Masahiro Goto
The technological utility of active pharmaceutical ingredients (APIs) is greatly enhanced when they are transformed into ionic liquids (ILs). API-ILs have better solubility, thermal stability, and the efficacy in topical delivery than solid or crystalline drugs. However, toxicological issue of API-ILs is the main challenge for their application in drug delivery. To address this issue, 11 amino acid esters (AAEs) were synthesized and investigated as biocompatible counter cations for the poorly water-soluble drug salicylic acid (Sal) to form Sal-ILs...
May 8, 2018: International Journal of Pharmaceutics
Le Zhang, Momei Zhou, Richard Stanton, Jeremy Kamil, Brent J Ryckman
Tropism of human cytomegalovirus (HCMV) is influenced by the envelope glycoprotein complexes gH/gL/gO and gH/gL/UL128-131. During virion assembly, gO and the UL128-131 proteins compete for binding to gH/gL in the ER. This assembly process clearly differs among strains since Merlin (ME) virions contain abundant gH/gL/UL128-131 and little gH/gL/gO, whereas TR contains much higher levels of total gH/gL, mostly in the form of gH/gL/gO, but much less gH/gL/UL128-131 than ME. Remaining questions include 1) what are the mechanisms behind these assembly differences, and 2) do differences reflect in vitro culture adaptations or natural genetic variations? Since the UL74(gO) ORF differs by 25% of amino acids between TR and ME, we analyzed recombinant viruses in which the UL74(gO) ORF was swapped...
May 9, 2018: Journal of Virology
Hanna B Scinto, Sandeep Gupta, Swati Thorat, Muhammad M Mukhtar, Anthony Griffiths, Jennifer Delgado, Elizabeth Plake, Hemant K Vyas, Amanda Strickland, Siddappa N Byrareddy, David C Montefiori, Celia LaBranche, Ranajit Pal, Jim Treece, Sharon Orndorff, Maria Grazia Ferrari, Deborah Weiss, Agnes-Laurence Chenine, Robert McLinden, Nelson Michael, Jerome H Kim, Merlin Robb, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Ruth M Ruprecht
The Phase III RV144 HIV vaccine trial conducted in Thailand remains the only study to show efficacy in decreasing HIV acquisition risk. In Thailand, circulating recombinant forms of HIV clades A/E (CRF01_AE) predominate; in such viruses, env originates from clade E (HIV-E). We constructed a simian-human immunodeficiency virus (SHIV) chimera carrying env isolated from an RV144 placebo recipient in the SHIV-1157ipd3N4 backbone. The latter contains long terminal repeats (LTRs) with duplicated NF-κB sites, thus resembling HIV LTRs...
May 9, 2018: Journal of Virology
Scott Grieshaber, Nicole Grieshaber, Hong Yang, Briana Baxter, Ted Hackstadt, Anders Omsland
Bacteria of the genus Chlamydia include the significant human pathogens C. trachomatis and C. pneumoniae All Chlamydiae are obligate intracellular parasites that depend on infection of a host cell and transition through a biphasic developmental cycle. Following host cell invasion by the infectious Elementary Body (EB), the pathogen transitions to the replicative but non-infectious Reticulate Body (RB). Differentiation of the RB back to the EB is essential to generate infectious progeny. While the EB form has historically been regarded as metabolically inert, maintenance of infectivity during incubation with specific nutrients has revealed active maintenance of the infectious phenotype...
May 7, 2018: Journal of Bacteriology
Elena Bartkiene, Vadims Bartkevics, Laura Elīna Ikkere, Iveta Pugajeva, Paulina Zavistanaviciute, Vita Lele, Modestas Ruzauskas, Jurga Bernatoniene, Valdas Jakstas, Dovile Klupsaite, Daiva Zadeike, Pranas Viskelis, Grazina Juodeikiene
The aim of this study was to evaluate the influence of ultrasonication, fermentation with Lactobacillus plantarum LUHS135 and Lactobacillus paracasei LUHS244, and different methods of dehydration on the chemical composition of bovine colostrum (BC), including the fatty acid and free amino acid profile and the content of micro- and macroelements. In addition, we analyzed the changes in lactic acid bacteria count, microbial contamination (aerobic mesophilic spore-forming bacteria, enterobacteria including Escherichia coli, and fungi/yeasts), the abundance of biogenic amines, and the concentration of nucleotide monophosphates...
May 2, 2018: Journal of Dairy Science
Lipeng Xiong, Wenwen Yan, Emmanuel Zubia, Yanqing Zhou, Yang Zhang, Qianqian Duan, Mahesh Narayan, Guoqiang Xu
A Disintegrin And Metalloproteinase 12 (ADAM12) is highly expressed in multiple cancers such as breast and cervical cancers and its high expression reduces the overall patient survival rate. ADAM12 has two major splicing variants, the long membrane-anchored form ADAM12L and the short secreted form ADAM12S. However, how they are regulated and whether they are modulated similarly or differently in cells are not clear. Here, we use affinity purification and mass spectrometry to identify the ADAM12S-interacting proteins...
May 2, 2018: Journal of Proteomics
Emmanuelle Schmitt, Gabrielle Bourgeois, Muriel Gondry, Alexey Aleksandrov
Cyclodipeptide synthases (CDPSs) form various cyclodipeptides from two aminoacyl tRNAs via a stepwise mechanism with the formation of a dipeptidyl enzyme intermediate. As a final step of the catalytic reaction, the dipeptidyl group undergoes intramolecular cyclization to generate the target cyclodipeptide product. In this work, we investigated the cyclization reaction in the cyclodipeptide synthase AlbC using QM/MM methods and free energy simulations. The results indicate that the catalytic Y202 residue is in its neutral protonated form, and thus, is not likely to serve as a general base during the reaction...
May 4, 2018: Scientific Reports
Ilia A Dereven'kov, Luciana Hannibal, Sergei V Makarov, Anna S Makarova, Pavel A Molodtsov, Oskar I Koifman
Serum albumin binds to a variety of endogenous ligands and drugs. Human serum albumin (HSA) binds to heme via hydrophobic interactions and axial coordination of the iron center by protein residue Tyr161. Human serum albumin binds to another tetrapyrrole, cobalamin (Cbl), but the structural and functional properties of this complex are poorly understood. Herein, we investigate the reaction between aquacobalamin (H2 OCbl) and bovine serum albumin (BSA, the bovine counterpart of HSA) using Ultraviolet-Visible and fluorescent spectroscopy, and electron paramagnetic resonance...
May 2, 2018: Journal of Biological Inorganic Chemistry: JBIC
Syang-Peng Rwei, Huynh Nguyen Anh Tuan, Whe-Yi Chiang, Tun-Fun Way
A series of semi-interpenetrating polymer network (semi-IPN) hydrogels were synthesized and investigated in this study. Linear copolymer poly( N -isopropylacrylamide-co-itaconamic acid) p(NIPAM-co-IAM), which is formed by copolymerization of N -isopropylacrylamide (NIPAM) and itaconamic acid (IAM, 4-amino-2-ethylene-4-oxobutanoic acid), was introduced into a solution of NIPAM to form a series of pH and thermo dual-responsive p(NIPAM-co-IAM)/pNIPAM semi-IPN hydrogels by free radical polymerization. The structural, morphological, chemical, and physical properties of the linear copolymer and semi-IPN hydrogels were investigated...
April 28, 2018: Materials
Ashok Kumar, Gaobing Wu, Ziduo Liu
A thermo-stable purified serine hydroxymethyltransferase (SHMT; 418 AA) was used for the carrier free immobilization using pectin as a coach molecule and formaldehyde as a cross-linker. The purified protein was cross linked with formaldehyde in the presence of pectin to form stable and active aggregates. The cross-linked enzyme aggregates [CLEAs] of SHMT showed improved catalytic properties and reusability. The SHMT-CLEAs showed a noteworthy change in the thermo-stability and activity compared to its free counterpart...
April 22, 2018: International Journal of Biological Macromolecules
Vasiliy I Vladimirov, Evgeni Yu Zernii, Viktoriia E Baksheeva, Hanna Wimberg, Alexey S Kazakov, Natalya K Tikhomirova, Ekaterina L Nemashkalova, Vladimir A Mitkevich, Andrey A Zamyatnin, Valery M Lipkin, Pavel P Philippov, Sergei E Permyakov, Ivan I Senin, Karl-W Koch, Dmitry V Zinchenko
Rod cell membranes contain cholesterol-rich detergent-resistant membrane (DRM) rafts, which accumulate visual cascade proteins as well as proteins involved in regulation of phototransduction such as rhodopsin kinase and guanylate cyclases. Caveolin-1 is the major integral component of DRMs, possessing scaffolding and regulatory activities towards various signaling proteins. In this study, photoreceptor Ca2+ -binding proteins recoverin, NCS1, GCAP1, and GCAP2, belonging to neuronal calcium sensor (NCS) family, were recognized as novel caveolin-1 interacting partners...
April 14, 2018: Cell Calcium
Chunho Kim, Intae Son, Jae Hong Kim, Ji Yong Yoo, Byungsun Lee, Chi Hyeong Cho, Jun Hyup Lee
Nanoscale self-assembled molecular layers of 1-pyrene carboxylic acid, 1-pyrene butylic acid, and 1-amino pyrene were employed as photoluminescent alignment agents to form vertical arrays of liquid crystals (LCs). The LC device using alignment agent of 1-amino pyrene appeared to have a stable vertical alignment based on crossed polarization analysis. The electro-optical properties of the LCs fabricated using this self-assembled layer exhibited better performance than those of general LC cells containing a conventional polyimide layer...
September 1, 2018: Journal of Nanoscience and Nanotechnology
Sergey Arkhipenko, Marco T Sabatini, Andrei S Batsanov, Valerija Karaluka, Tom D Sheppard, Henry S Rzepa, Andrew Whiting
The generally accepted monoacyloxyboron mechanism of boron-catalysed direct amidation is brought into question in this study, and new alternatives are proposed. We have carried out a detailed investigation of boron-catalysed amidation reactions, through study of the interaction between amines/carboxylic acids and borinic acids, boronic acids and boric acid, and have isolated and characterised by NMR/X-ray crystallography many of the likely intermediates present in catalytic amidation reactions. Rapid reaction between amines and boron compounds was observed in all cases, and it is proposed that such boron-nitrogen interactions are highly likely to take place in catalytic amidation reactions...
January 28, 2018: Chemical Science
Dicky Pranantyo, Li Qun Xu, En-Tang Kang, Mary B Chan-Park
The rapid spread of multidrug resistant bacteria has called for effective antimicrobial agents which work on a more direct mechanism of killing. Cationic peptidopolysaccharides are developed in the present work to mimic the peptidoglycan structure of bacteria and to enhance the membrane-compromising bactericidal efficacy. Antimicrobial CysHHC10 peptide was grafted to the C-2 (amino) or C-6 (hydroxyl) position of chitosan backbone via thiol-maleimide 'click' conjugation, utilizing the maleimidohexanoic linkers...
April 19, 2018: Biomacromolecules
Yifeng Wang, Jibiao Li, David Matye, Yuxia Zhang, Katie Dennis, Wen-Xing Ding, Tiangang Li
Bile acids are signaling molecules that critically control hepatocellular function. Disrupted bile acid homeostasis may be implicated in the pathogenesis of chronic liver diseases. Glutathione is an important antioxidant that protects the liver against oxidative injury. Various forms of liver disease share the common characteristics of reduced cellular glutathione and elevated oxidative stress. This study reports a potentially novel physiological function of bile acids in regulating hepatic sulfur amino acid and glutathione metabolism...
April 19, 2018: JCI Insight
Xin Yin, Dong Ying, Sébastien Lhomme, Zimin Tang, Christopher M Walker, Ningshao Xia, Zizheng Zheng, Zongdi Feng
The enterically transmitted hepatitis E virus (HEV) adopts a unique strategy to exit cells by cloaking its capsid (encoded by the viral ORF2 gene) and circulating in the blood as "quasi-enveloped" particles. However, recent evidence suggests that the majority of the ORF2 protein present in the patient serum and supernatants of HEV-infected cell culture exists in a free form and is not associated with virus particles. The origin and biological functions of this secreted form of ORF2 (ORF2S ) are unknown...
May 1, 2018: Proceedings of the National Academy of Sciences of the United States of America
Chenchen Wang, Ying Tong, Yankai Wen, Jie Cai, Han Guo, Lifeng Huang, Min Xu, Mingxuan Feng, Xiaosong Chen, Jianjun Zhang, Hailong Wu, Xiaoni Kong, Qiang Xia
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death worldwide. Increased lipogenesis has been reported to play a critical role in HCC progression. However, the underlying mechanism contributing to lipogenesis increase in HCC remains elusive. Here, we show that hepatocellular carcinoma-associated protein TD26 (TD26) was highly expressed in HCC tumor tissues compared to matched normal tissues. From the clinicopathologic analyses of two independent HCC cohorts, we demonstrate that TD26 expression was positively correlated with tumor size and was an independent predictor of overall survival (OS) and recurrence free survival (RFS) in HCC patients...
April 16, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
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