Karine Anselme, Odile Broux, Benoit Noel, Bertrand Bouxin, Gerard Bascoulergue, Anne-France Dudermel, Fabien Bianchi, Joseph Jeanfils, Pierre Hardouin
For the clinical application of cultured human mesenchymal stem cells (MSCs), cells must have minimal contact with fetal calf serum (FCS) because it might be a potential vector for contamination by adventitious agents. The use of human plasma and serum for clinical applications also continues to give rise to considerable concerns with respect to the transmission of known and unknown human infectious agents. With the objective of clinical applications of cultured human MSCs, we tested the ability of autologous plasma, AB human serum, FCS, and artificial serum substitutes containing animal-derived proteins (Ultroser G) or vegetable-derived proteins (Prolifix S6) to permit their growth and differentiation in vitro...
December 2002: Tissue Engineering