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tissue engineered vascular graft

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https://www.readbyqxmd.com/read/28717129/3d-printed-scaffolds-of-calcium-silicate-doped-%C3%AE-tcp-synergize-with-co-cultured-endothelial-and-stromal-cells-to-promote-vascularization-and-bone-formation
#1
Yuan Deng, Chuan Jiang, Cuidi Li, Tao Li, Mingzheng Peng, Jinwu Wang, Kerong Dai
Synthetic bone scaffolds have potential application in repairing large bone defects, however, inefficient vascularization after implantation remains the major issue of graft failure. Herein, porous β-tricalcium phosphate (β-TCP) scaffolds with calcium silicate (CS) were 3D printed, and pre-seeded with co-cultured human umbilical cord vein endothelial cells (HUVECs) and human bone marrow stromal cells (hBMSCs) to construct tissue engineering scaffolds with accelerated vascularization and better bone formation...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28680888/early-graft-tunnel-healing-after-anterior-cruciate-ligament-reconstruction-with-intratunnel-injection-of-bone-marrow-mesenchymal-stem-cells-and-vascular-endothelial-growth-factor
#2
Rosy Setiawati, Dwikora Novembri Utomo, Fedik Abdul Rantam, Nadia Nastassia Ifran, Nicolaas C Budhiparama
BACKGROUND: Bone marrow mesenchymal stem cells (BM-MSCs) are multipotent adult stem cells and have become an important source of cells for engineering tissue repair and cell therapy. Vascular endothelial growth factor (VEGF) promotes angiogenesis and contributes fibrous integration between tendon and bone during the early postoperative stage. Both MSCs and VEGF can stimulate cell proliferation, differentiation, and matrix deposition by enhancing angiogenesis and osteogenesis of the graft in the tunnel...
June 2017: Orthopaedic Journal of Sports Medicine
https://www.readbyqxmd.com/read/28674697/cell-free-vascular-grafts-recent-developments-and-clinical-potential
#3
Sindhu Row, Ana Santandreu, Daniel D Swartz, Stelios T Andreadis
Recent advances in vascular tissue engineering have led to the development of cell-free grafts that are available off-the-shelf for on demand surgery. Challenges associated with cell-based technologies including cell sourcing, cell expansion and long-term bioreactor culture motivated the development of completely cell-free vascular grafts. These are based on decellularized arteries, decellularized cultured cell-based tissue engineered grafts or biomaterials functionalized with biological signals that promote in situ tissue regeneration...
March 2017: Technology
https://www.readbyqxmd.com/read/28671618/vascular-mechanobiology-towards-control-of-in-situ-regeneration
#4
REVIEW
Eline E van Haaften, Carlijn V C Bouten, Nicholas A Kurniawan
The paradigm of regenerative medicine has recently shifted from in vitro to in situ tissue engineering: implanting a cell-free, biodegradable, off-the-shelf available scaffold and inducing the development of functional tissue by utilizing the regenerative potential of the body itself. This approach offers a prospect of not only alleviating the clinical demand for autologous vessels but also circumventing the current challenges with synthetic grafts. In order to move towards a hypothesis-driven engineering approach, we review three crucial aspects that need to be taken into account when regenerating vessels: (1) the structure-function relation for attaining mechanical homeostasis of vascular tissues, (2) the environmental cues governing cell function, and (3) the available experimental platforms to test instructive scaffolds for in situ tissue engineering...
July 3, 2017: Cells
https://www.readbyqxmd.com/read/28667291/a-biomimetic-heparinized-composite-silk-based-vascular-scaffold-with-sustained-antithrombogenicity
#5
Masoud Zamani, Mona Khafaji, Mohammad Naji, Manouchehr Vossoughi, Iran Alemzadeh, Nooshin Haghighipour
Autologous grafts, as the gold standard for vascular bypass procedures, associated with several problems that limit their usability, so tissue engineered vessels have been the subject of an increasing number of works. Nevertheless, gathering all of the desired characteristics of vascular scaffolds in the same construct has been a big challenge for scientists. Herein, a composite silk-based vascular scaffold (CSVS) was proposed to consider all the mechanical, structural and biological requirements of a small-diameter vascular scaffold...
June 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28665233/decellularization-of-whole-human-liver-grafts-using-controlled-perfusion-for-transplantable-organ-bioscaffolds
#6
Monique Verstegen, Jorke Willemse, Sjoerd van den Hoek, Gert-Jan Kremers, Theo M Luider, Nick A van Huizen, Francois E J A Willemssen, Herold J Metselaar, Jan Nm IJzermans, Luc J W van der Laan, Jeroen de Jonge
Liver transplantation is the only effective treatment for end-stage liver disease, but absolute donor shortage remains a limiting factor. Recent advances in tissue engineering focus on generation of native extracellular matrix (ECM) by decellularized complete livers in animal models. Though proof of concept has been reported for human livers, the current study aims to perform whole liver decellularization in a clinically relevant series using controlled machine perfusion. Here we describe a mild non-destructive decellularization protocol, effective in 11 discarded human whole liver grafts to generate constructs that reliably maintain hepatic architecture and ECM components using machine perfusion, while completely removing cellular DNA and RNA...
June 30, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-eptfe-graft-sodding
#7
Stuart K Williams, Marvin E Morris, Paul Kosnik, Kevin D Lye, Gary Gentzkow, Charles B Ross, Amit J Dwivedi, Leigh B Kleinert
Adipose-derived stromal vascular fraction cell populations are being evaluated for numerous clinical applications. The current study evaluated a point of care (POC) technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived stromal vascular fraction cells to be used in the fabrication of a tissue engineered vascular graft in the operating room. A total of 7 patients were enrolled in this FDA registered Investigational Device Exemption clinical trial and received femoral to tibial expanded polytetrafluoroethylene (ePTFE) bypass grafts to treat peripheral arterial disease...
June 28, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28643432/the-penetration-and-phenotype-modulation-of-smooth-muscle-cells-on-surface-heparin-modified-poly-%C3%AE%C2%B5-caprolactone-vascular-scaffold
#8
Jie Cao, Xue Geng, Juan Wen, Qingxuan Li, Lin Ye, Aiying Zhang, Zengguo Feng, Lianrui Guo, Yongquan Gu
The tubular porous poly(ε-caprolactone)(PCL) scaffold was fabricated by electrospinning. After then, the scaffold's surface was firstly eroded by hexyldiamine to endow amine group, and heparin was covalently grafted to the surface to get surface heparin modified scaffold(ShPCL scaffold). It was found that ShPCL scaffold can induce smooth muscle cells (SMCs) to penetrate the scaffold surface, while the SMCs can't penetrate the surface of PCL scaffold. Subsequently, the rabbit SMCs were seeded on the ShPCL scaffold and cultured for 14 days...
June 23, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28642016/electrospun-vascular-scaffold-for-cellularized-small-diameter-blood-vessels-a-preclinical-large-animal-study
#9
Young Min Ju, Hyunhee Ahn, Juan Arenas-Herrera, Cheil Kim, Mehran Abolbashari, Anthony Atala, James J Yoo, Sang Jin Lee
The strategy of vascular tissue engineering is to create a vascular substitute by combining autologous vascular cells with a tubular-shaped biodegradable scaffold. We have previously developed a novel electrospun bilayered vascular scaffold that provides proper biological and biomechanical properties as well as structural configuration. In this study, we investigated the clinical feasibility of a cellularized vascular scaffold in a preclinical large animal model. We fabricated the cellularized vascular construct with autologous endothelial progenitor cell (EPC)-derived endothelial cells (ECs) and smooth muscle cells (SMCs) followed by a pulsatile bioreactor preconditioning...
June 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28604360/chitosan-based-hydrogels-for-developing-a-small-diameter-vascular-graft-in-vitro-and-in-vivo-evaluation
#10
Audrey Aussel, Noélie Thébaud, Xavier Berard, Vincenzo Brizzi, Samantha Delmond, Reine Bareille, Robin Siadous, Chloe James, Jean Ripoche, Marlène Durand, Alexandra Montembault, Béatrice Burdin, Didier Letourneur, Nicolas L'Heureux, Laurent David, Laurence Bordenave
Vascular grafts made of synthetic polymers perform poorly in small-diameter applications (cardiac and peripheral bypass). Chitosan is a biocompatible natural polymer that can provide a novel biological scaffold for tissue engineering development. The goal of this study was to demonstrate the biocompatibility of a novel chitosan preparation in vitro and in vivo, and to assess its potential as a scaffold for vascular applications. Methods and Results. A series of experiments of increasing complexity, ranging from in vitro biocompatibility and hemocompatibility tests to in vivo studies in small and large animals (rat and sheep), was performed to provide a comprehensive analysis of chitosan hydrogels' biological properties...
June 12, 2017: Biomedical Materials
https://www.readbyqxmd.com/read/28576046/fabrication-of-self-assembled-vesicle-nanoparticles-of-poly-l-lysine-arachidic-acid-conjugates-for-a-vascular-endothelial-growth-factor-carrier
#11
Po-Chung Chen, Adhimoorthy Prasannan, Chun-Chiang Huang, Shu-Fang Tang, Hsieh Chih Tsai
Numerous growth factors account for tissue and organ development and therapeutic efficiency. Hence, the delivery of growth factors is crucial in regenerative medical practice. However, the delivery of a single factor to regenerate tissues lacks clinical utility in many current approaches. We reported the delivery of the bioactive vascular endothelial growth factor (VEGF) from novel polymeric vesicles. Polymeric vesicles were prepared from the poly(l-lysine)-g-polylysine(AA) amphiphilic graft copolymer through the conjugation of arachidic acid (AA) with poly(l-lysine) for obtaining a VEGF carrier...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28569644/microwave-assisted-facile-fabrication-of-porous-poly-glycerol-sebacate-scaffolds
#12
Soo Hyon Lee, Kee-Won Lee, Piyusha S Gade, Anne M Robertson, Yadong Wang
The biodegradable elastomeric polyester poly(glycerol sebacate) (PGS) was developed for soft-tissue engineering. It has been used in various research applications such as wound healing, cartilage tissue engineering, and vascular grafting due to its biocompatibility and elastomeric properties. However conventional PGS manufacture is generally limited by the laborious reaction conditions needed for curing which requires elevated reaction temperatures, high vacuum and multi-day reaction times. In this study, we developed a microwave irradiation methodology to fabricate PGS scaffolds under milder conditions with curing times that are 8 times faster than conventional methods...
June 16, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28544815/fabrication-of-viable-and-functional-pre-vascularized-modular-bone-tissues-by-coculturing-mscs-and-huvecs-on-microcarriers-in-spinner-flasks
#13
Songjie Zhang, Min Zhou, Zhaoyang Ye, Yan Zhou, Wen-Song Tan
BACKGROUND AND AIM: Slow vascularization often impedes the viability and function of engineered bone replacements. Prevascularization is a promising way to solve this problem. In this study, a new process was developed by integrating microcarrier culture and coculture to fabricate pre-vascularized bone microtissues with mesenchymal stem cells (MSCs) and human umbilical vein endotheli-al cells (HUVECs). METHODOLOGY: Initially, coculture medium and cell ratio between MSCs and HUVECs were optimized in tissue culture plates concerning cell proliferation, osteogenesis and angiogenesis...
May 24, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28524773/the-role-of-myeloid-cell-derived-pdgf-b-in-neotissue-formation-in-a-tissue-engineered-vascular-graft
#14
Ekene Onwuka, Cameron Best, Andrew Sawyer, Tai Yi, Eric Heuer, Malik Sams, Matthew Wiet, Hong Zheng, Themis Kyriakides, Christopher Breuer
AIM: Inflammatory myeloid lineage cells mediate neotissue formation in tissue-engineered vascular grafts, but the molecular mechanism is not completely understood. We examined the role of vasculogenic PDGF-B in tissue-engineered vascular graft neotissue development. MATERIALS & METHODS: Myeloid cell-specific PDGF-B knockout mice (PDGF-KO) were generated using bone marrow transplantation, and scaffolds were implanted as inferior vena cava interposition grafts in either PDGF-KO or wild-type mice...
May 19, 2017: Regenerative Medicine
https://www.readbyqxmd.com/read/28494781/preparation-and-characterization-of-small-diameter-decellularized-scaffolds-for-vascular-tissue-engineering-in-an-animal-model
#15
Shuangyue Xu, Fangna Lu, Lianna Cheng, Chenglin Li, Xu Zhou, Yuan Wu, Hongxing Chen, Kaichuang Zhang, Lumin Wang, Junjie Xia, Guoliang Yan, Zhongquan Qi
BACKGROUND: The development of a suitable extracellular matrix (ECM) scaffold is the first step in vascular tissue engineering (VTE). Synthetic vascular grafts are available as an alternative to autologous vessels in large-diameter arteries (>8 mm) and medium-diameter arteries (6-8 mm). In small-diameter vessels (<6 mm), synthetic vascular grafts are of limited use due to poor patency rates. Compared with a vascular prosthesis, natural tissue ECM has valuable advantages. Despite considerable progress in recent years, identifying an optimal protocol to create a scaffold for use in small-diameter (<6 mm) fully natural tissue-engineered vascular grafts (TEVG), remains elusive...
May 11, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28486019/role-of-bone-marrow-mononuclear-cell-seeding-for-nanofiber-vascular-grafts
#16
Takuma Fukunishi, Cameron A Best, Chin Siang Ong, Tyler Groehl, James Reinhardt, Tai Yi, Hideki Miyachi, Huaitao Zhang, Toshiharu Shinoka, Christopher K Breuer, Jed Johnson, Narutoshi Hibino
OBJECTIVE: Electrospinning is a promising technology that provides biodegradable nanofiber scaffolds for cardiovascular tissue engineering. However, success with these materials has been limited, and the optimal combination of scaffold parameters for a tissue-engineered vascular graft (TEVG) remains elusive. The purpose of the present study is to evaluate the effect of bone marrow mononuclear cell (BM-MNC) seeding in electrospun scaffolds to support the rational design of optimized TEVGs...
June 13, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28485303/synthesis-of-cell-composite-alginate-microfibers-by-microfluidics-with-the-application-potential-of-small-diameter-vascular-grafts
#17
Mingying Liu, Zhizhi Zhou, Yongchao Chai, Shuai Zhang, Xiaomeng Wu, Shaopeng Huang, Jiaqiang Su, Jiahuan Jiang
Fabrication of small diameter vascular grafts (SDVGs) with appropriate responses for clinical application is still challenging. In the present work, the production and characterization of solid alginate based microfibers as potential SDVG candidates through the method of microfluidics were considered original. A simple glass microfluidic device with a 'L-shape' cylindrical-flow channel in the microfluidic platform was developed. The gelation of microfibers occurred when the alginate solution and a CaCl2 solution were introduced as a core flow and as a sheath flow, respectively...
June 7, 2017: Biofabrication
https://www.readbyqxmd.com/read/28467648/building-better-bone-the-weaving-of-biologic-and-engineering-strategies-for-managing-bone-loss
#18
Andrew M Schwartz, Mara L Schenker, Jaimo Ahn, Nick J Willett
Segmental bone loss remains a challenging clinical problem for orthopaedic trauma surgeons. In addition to the missing bone itself, the local tissues (soft tissue, vascular) are often highly traumatized as well, resulting in a less than ideal environment for bone regeneration. As a result, attempts at limb salvage become a highly expensive endeavor, often requiring multiple operations and necessitating the use of every available strategy (autograft, allograft, bone graft substitution, Masquelet, bone transport, etc...
May 3, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28457188/functional-tissue-engineering-a-prevascularized-cardiac-muscle-construct-for-validating-human-mesenchymal-stem-cells-engraftment-potential-in-vitro
#19
Mani T Valarmathi, John W Fuseler, Jay D Potts, Jeffrey M Davis, Robert L Price
The influence of somatic stem cells in the stimulation of mammalian cardiac muscle regeneration is still in its early stages, and so far, it has been difficult to determine the efficacy of the procedures that have been employed. The outstanding question remains whether stem cells derived from the bone marrow or some other location within or outside of the heart can populate a region of myocardial damage and transform into tissue-specific differentiated progenies, and also exhibit functional synchronization...
May 25, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28447994/scaling-of-engineered-vascular-grafts-using-3d-printed-guides-and-the-ring-stacking-method
#20
Cameron B Pinnock, Zhengfan Xu, Mai T Lam
Coronary artery disease remains a leading cause of death, affecting millions of Americans. With the lack of autologous vascular grafts available, engineered grafts offer great potential for patient treatment. However, engineered vascular grafts are generally not easily scalable, requiring manufacture of custom molds or polymer tubes in order to customize to different sizes, constituting a time-consuming and costly practice. Human arteries range in lumen diameter from about 2.0-38 mm and in wall thickness from about 0...
March 27, 2017: Journal of Visualized Experiments: JoVE
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