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tissue engineered vascular graft

M Gasser, M H Frank, A M Waaga-Gasser
Critical chronic ischemia in patients with underlying arterial occlusive disease requires vascular reconstructive surgery. The limited supply of suitable small-diameter autologous vascular grafts in many patients and obvious disadvantages of synthetic bypass material demand the development of clinically usable tissue-engineered blood vessel substitutes. Despite substantial progress in the field over the last two decades, their implementation into the clinical routine has been challenging. The limited replicative life span of human adult vascular cells and their slow rate of collagenous matrix production in vitro have posed important problems in the development of mechanically robust and biologically functional engineered grafts...
February 2018: Gefässchirurgie: Zeitschrift Für Vaskuläre und Endovaskuläre Chirurgie
Santiago Roura, Carolina Gálvez-Montón, Antoni Bayes-Genis
Chronic diseases, including myocardial scar healing and heart failure remission, impose huge social and economic burdens, and novel approaches are needed. Several therapeutic modalities are currently being evaluated, including cell therapy, stem cell conditioning, and cardiac tissue engineering. Areas covered: This review discusses the restoration of cardiac function after myocardial infarction using a vascularized flap of autologous cardiac adipose tissue over an akinetic scar. It addresses the risks and benefits of using cardiac adipose progenitors and the adipose graft transposition procedure (AGTP) to ameliorate cardiac dysfunction in preclinical and clinical trials...
March 6, 2018: Expert Review of Cardiovascular Therapy
Agnieszka A Ksiazek, Laura Frese, Petra E Dijkman, Bart Sanders, Sarah E Motta, Benedikt Weber, Simon P Hoerstrup
Data on in vitro engineered "off the shelf" matrices support the concept of endogenous cellular repopulation driving the graft's remodeling via immune-mediated response. This seems important to further accelerate the cell reconstitution and may play a crucial role when mononuclear cells are used. Nevertheless, studies on decellularized xenogeneic grafts showed only limited host cell repopulation post-implantation. This study aims at a systematic comparison of reseeding methods (dripping, injection, bathing in a cell suspension and combined puncturing-dripping method) to define the most efficient technique enhancing recellularization of tissue engineered vascular matrices (patches, vessels, small diameter and standard size valves) prior implantation...
March 2, 2018: Acta Biomaterialia
Anton G Kutikhin, Maxim Yu Sinitsky, Arseniy E Yuzhalin, Elena A Velikanova
Among applicable high-throughput techniques in cardiovascular biology, whole-transcriptome sequencing is of particular use. By utilizing RNA that is isolated from virtually all cells and tissues, the entire transcriptome can be evaluated. In comparison with other high-throughput approaches, RNA sequencing is characterized by a relatively low-cost and large data output, which permits a comprehensive analysis of spatiotemporal variation in the gene expression profile. Both shear stress and cyclic strain exert hemodynamic force upon the arterial endothelium and are considered to be crucial determinants of endothelial physiology...
February 21, 2018: High Throughput
Juliana Jaramillo, Karen T Valencia-Rivero, Francisco J Cedano-Serrano, Rocío López, Néstor Sandoval, Juan C Briceño
Synthetic vascular access for hemodialysis exhibits biological and mechanical material properties mismatch with the native vessels. These limitations prevent infiltration of endothelial cells and decrease grafts long-term patency, particularly in small diameter vessels. We aimed to design a curved structural reinforced small intestinal submucosa (SIS) vascular graft for hemodialysis access and to evaluate in a porcine animal model graft patency by Doppler ultrasonography, tissue remodeling by histology, and vascular wall Young's modulus after implantation by biaxial tensile test...
March 2018: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Bijal Patel, Zhengfan Xu, Cameron B Pinnock, Loay S Kabbani, Mai T Lam
Efforts for tissue engineering vascular grafts focuses on the tunica media and intima, although the tunica adventitia serves as the primary structural support for blood vessels. In surgery, during endarterectomies, surgeons can strip the vessel, leaving the adventitia as the main strength layer to close the vessel. Here, we adapted our recently developed technique of forming vascular tissue rings then stacking the rings into a tubular structure, to accommodate human fibroblasts to create adventitia vessels in 8 days...
February 19, 2018: Scientific Reports
Anh La, Robert T Tranquillo
Conferring anti-thrombogenicity to tissue-engineered vascular grafts remains a major challenge, especially for urgent bypass grafting that excludes approaches based on expanding autologous endothelial cells that requires weeks of cell culture. Adipose-derived stem cells are available from most patients in sufficient number for coronary bypass graft seeding and may be effective as allogeneic cells. We thus compared the adhesion and platelet binding of human adipose-derived stem cells that were shear-conditioned with steady and pulsatile shear stress after seeding the cells on a biologically-engineered matrix suitable for arterial grafts...
February 16, 2018: Tissue Engineering. Part A
Yan-Yun Wu, Yan-Peng Jiao, Li-Ling Xiao, Min-Min Li, Hong-Wei Liu, Sheng-Hong Li, Xuan Liao, Yong-Tian Chen, Jiang-Xuan Li, Yang Zhang
BACKGROUND: Wound healing is a complex process that relies on growth factors and stimulation of angiogenesis. Tissue engineering materials composed of adipose-derived stem cells (ADSCs) and silk fibroin (SF)/chitosan (CS) may be able to solve this problem. The aim of this study was to investigate the wound-healing potential of ADSC-seeded SF/CS in streptozotocin-induced diabetic rats. MATERIALS AND METHODS: Thirty-six male Sprague-Dawley rats were purchased and randomly assigned into 3 groups: a control group (no graft), a group treated with SF/CS film graft, and a group treated with ADSC-seeded SF/CS graft...
February 13, 2018: Annals of Plastic Surgery
Cameron A Best, Takuma Fukunishi, Joseph Drews, Ramak Khosravi, Kan Hor, Nathan Mahler, Tai Yi, Jay D Humphrey, Jed Johnson, Christopher K Breuer, Narutoshi Hibino
Most tissue-engineered arterial grafts are complicated by aneurysmal dilation secondary to insufficient neotissue formation after scaffold degradation. The optimal graft would form an organized multi-layered structure with a robust extracellular matrix that could withstand arterial pressure. The purpose of the current study was to determine how oversizing a biodegradable arterial scaffold affects long-term neotissue formation. Size-matched (1.0 mm, n=11) and oversized (1.6 mm, n=9) electrospun polycaprolactone/chitosan scaffolds were implanted as abdominal aortic interposition grafts in Lewis rats...
February 12, 2018: Tissue Engineering. Part A
Tadahisa Sugiura, Goki Matsumura, Shinka Miyamoto, Hideki Miyachi, Christopher K Breuer, Toshiharu Shinoka
OBJECTIVE: Tissue engineering holds great promise for the advancement of cardiovascular surgery as well as other medical fields. Tissue-engineered vascular grafts have the ability to grow and remodel and could therefore make great advances for pediatric cardiovascular surgery. In 2001, we began a human clinical trial evaluating these grafts in patients with a univentricular physiology. Herein, we report the long-term results of patients who underwent implantation of tissue-engineered vascular grafts as extracardiac total cavopulmonary conduits...
February 7, 2018: Seminars in Thoracic and Cardiovascular Surgery
Huijie Jianga, Pengzhen Chenga, Donglin Lia, Junqin Lia, Jimeng Wanga, Yi Gaoa, Shuaishuai Zhanga, Tianqing Caoa, Chunmei Wanga, Liu Yanga, Guoxian Peia
Massive bone defects are a challenge in orthopedic research. Defective regeneration leads to bone atrophy, non-union of bone and physical morbidity. Large animals are important models, however, production costs are high, nursing is complex and evaluation methods are limited. A suitable laboratory animal model is required to explore the underlying molecular mechanism and cellular process of bone tissue engineering. We designed a stainless steel plate with eight holes; the middle two holes were used as a guide to create a standardized critical size defect in the femur of anesthetized rats...
February 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
Jhilmil Dhulekar, Agneta Simionescu
Hyperglycemia and dyslipidemia coexist in diabetes and result in inflammation, degeneration, and impaired tissue remodeling, processes which are not conducive to the desired integration of tissue engineered products into the surrounding tissues. There are several challenges for vascular tissue engineering such as non-thrombogenicity, adequate burst pressure and compliance, suturability, appropriate remodeling responses, and vasoactivity, but, under diabetic conditions, an additional challenge needs to be considered: the aggressive oxidative environment generated by the high glucose and lipid concentrations that lead to the formation of advanced glycation end products (AGEs) in the vascular wall...
January 25, 2018: Acta Biomaterialia
Hao Zhao, Yan Zhao, Zili Li, Qi Ouyang, Yi Sun, Di Zhou, Pingyuan Xie, Sicong Zeng, Lingfeng Dong, Hua Wen, Guangxiu Lu, Ge Lin, Liang Hu
Rationale-endothelial cells (ECs) play important roles in various regeneration processes and can be used in a variety of therapeutic applications, such as cardiac regeneration, gene therapy, tissue-engineered vascular grafts and prevascularized tissue transplants. ECs can be acquired from pluripotent and adult stem cells. To acquire ECs from human embryonic stem cells (hESCs) in a fast, efficient and economic manner. We established a conditional overexpression system in hESCs based on 15 transcription factors reported to be responsible for hematopoiesis lineage...
January 26, 2018: Cell Death & Disease
Fabiana Zanata, Annie Bowles, Trivia Frazier, J Lowry Curley, Bruce A Bunnell, Xiying Wu, James Wade, Ram Devireddy, Jeffrey M Gimble, Lydia Masako Ferreira
BACKGROUND: Adipose tissue is a source of adipose-derived stromal/stem cells for tissue engineering and reconstruction and a tissue source for fat grafts. Although liposuction is a simple procedure for the harvest of adipose tissue, the repetition of this surgical intervention can cause adverse effects to the patient and can be a limiting factor for immediate use. Cryopreservation can avoid the morbidity associated with repetitive liposuction, allowing the use of stored tissue after the initial harvest procedure...
February 2018: Plastic and Reconstructive Surgery
Eva Gonzalez Diaz, Yuru Shih, Manando Nakasaki, Mengqian Liu, Shyni Varghese
Synthetic biomaterials that create a dynamic calcium (Ca2+), phosphate (PO43-) ions, and calcium phosphate (CaP)-rich microenvironment, similar to that found in native bone tissue, have been shown to promote osteogenic commitment of stem cells in vitro and in vivo. The intrinsic osteoconductivity and osteoinductivity of such biomaterials makes them promising bone grafts for the treatment of bone defects. We thus aimed to evaluate the potential of mineralized biomaterials to induce bone repair of a critical-sized cranial defect in the absence of exogenous cells and growth factors...
January 25, 2018: Tissue Engineering. Part A
Dominik Siallagan, Yue-Hin Loke, Laura Olivieri, Justin Opfermann, Chin Siang Ong, Diane de Zélicourt, Anastasios Petrou, Marianne Schmid Daners, Vartan Kurtcuoglu, Mirko Meboldt, Kevin Nelson, Luca Vricella, Jed Johnson, Narutoshi Hibino, Axel Krieger
BACKGROUND: Despite advances in the Fontan procedure, there is an unmet clinical need for patient-specific graft designs that are optimized for variations in patient anatomy. The objective of this study is to design and produce patient-specific Fontan geometries, with the goal of improving hepatic flow distribution (HFD) and reducing power loss (Ploss), and manufacturing these designs by electrospinning. METHODS: Cardiac magnetic resonance imaging data from patients who previously underwent a Fontan procedure (n = 2) was used to create 3-dimensional models of their native Fontan geometry using standard image segmentation and geometry reconstruction software...
December 5, 2017: Journal of Thoracic and Cardiovascular Surgery
Mattia Francesco Maria Gerli, Jacques Paul Guyette, Daniele Evangelista-Leite, Brian Burns Ghoshhajra, Harald Christian Ott
Muscle and fasciocutaneous flaps taken from autologous donor sites are currently the most utilized approach for trauma repair, accounting annually for 4.5 million procedures in the US alone. However, the donor tissue size is limited and the complications related to these surgical techniques lead to morbidities, often involving the donor sites. Alternatively, recent reports indicated that extracellular matrix (ECM) scaffolds boost the regenerative potential of the injured site, as shown in a small cohort of volumetric muscle loss patients...
2018: PloS One
Dong Wang, LeeAnn K Li, Tiffany Dai, Aijun Wang, Song Li
Understanding the contribution of vascular cells to blood vessel remodeling is critical for the development of new therapeutic approaches to cure cardiovascular diseases (CVDs) and regenerate blood vessels. Recent findings suggest that neointimal formation and atherosclerotic lesions involve not only inflammatory cells, endothelial cells, and smooth muscle cells, but also several types of stem cells or progenitors in arterial walls and the circulation. Some of these stem cells also participate in the remodeling of vascular grafts, microvessel regeneration, and formation of fibrotic tissue around biomaterial implants...
2018: Theranostics
Frederic Wolf, Diana M Rojas González, Ulrich Steinseifer, Markus Obdenbusch, Werner Herfs, Christian Brecher, Stefan Jockenhoevel, Petra Mela, Thomas Schmitz-Rode
In vitro tissue engineering of vascular grafts requires dynamic conditioning in a bioreactor system for in vitro tissue maturation and remodeling to receive a mechanically adequate and hemocompatible implant. The goal of the current work was to develop a bioreactor system for the conditioning of vascular grafts which is (i) able to create a wide range of flow, pressure and frequency conditions, including physiological ones; (ii) compact and easy to assemble; (iii) transportable; (iv) disposable. The system is driven by a small centrifugal pump controlled via a custom-made control unit, which can also be operated on batteries to allow for autonomous transportation...
January 16, 2018: Annals of Biomedical Engineering
Xuewen Qiu, Jiahan Wang, Guifang Wang, Huangdin Wen
The Lando® dermal scaffold is a newly developed, tissue-engineered dermal scaffold material. This study sought to observe its vascularization in an acute full-thickness skin-defect porcine model. There were eight Tibetan pigs in this research. Six 5 × 5 cm full-thickness skin-defect wounds were prepared on the dorsal area of each pig, which were divided into two groups. The experimental group wounds were covered by Lando® dermal scaffolds, while the other received Vaseline gauzes as blank control. At day 3, 7, 14 and 21 after injury, the general condition of wounds was observed, and wound specimens were obtained for HE staining, Masson staining and the expression of CD31, α-SMA and VEGF, which were examined by immunohistochemistry...
January 10, 2018: Journal of Plastic Surgery and Hand Surgery
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