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tissue engineered blood vessel

Tianlin Liu, Xin Zhang, Yuan Luo, Yuanliang Huang, Gang Wu
Bone tissue engineering technique is a promising strategy to repair large-volume bone defects. In this study, we developed a 3-dimensional construct by combining icariin (a small-molecule Chinese medicine), allogeneic bone marrow-derived mesenchymal stem cells (BMSCs), and a siliceous mesostructured cellular foams-poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (SMC-PHBHHx) composite scaffold. We hypothesized that the slowly released icariin could significantly promote the efficacy of SMC-PHBHHx/allogeneic BMSCs for repairing critical-size bone defects in rats...
2016: Stem Cells International
Valeria Perugini, Anna L Guildford, Joana Silva-Correia, Joaquim M Oliveira, Steven T Meikle, Rui L Reis, Matteo Santin
Damage of non-vascularised tissues such as cartilage and cornea can result in healing processes accompanied by a non-physiological angiogenesis. Peptidic aptamers have recently been reported to block the vascular endothelial growth factor (VEGF). However, the therapeutic applications of these aptamers is limited due to their short half-life in vivo. In this work, an enhanced stability and bioavailability of a known VEGF blocker aptamer sequence (WHLPFKC) was pursued through its tethering of molecular scaffolds based on hyperbranched peptides, the poly(ɛ-lysine) dendrons, bearing three branching generations...
October 8, 2016: Journal of Tissue Engineering and Regenerative Medicine
Yu Shrike Zhang, Andrea Arneri, Simone Bersini, Su-Ryon Shin, Kai Zhu, Zahra Goli-Malekabadi, Julio Aleman, Cristina Colosi, Fabio Busignani, Valeria Dell'Erba, Colin Bishop, Thomas Shupe, Danilo Demarchi, Matteo Moretti, Marco Rasponi, Mehmet Remzi Dokmeci, Anthony Atala, Ali Khademhosseini
Engineering cardiac tissues and organ models remains a great challenge due to the hierarchical structure of the native myocardium. The need of integrating blood vessels brings additional complexity, limiting the available approaches that are suitable to produce integrated cardiovascular organoids. In this work we propose a novel hybrid strategy based on 3D bioprinting, to fabricate endothelialized myocardium. Enabled by the use of our composite bioink, endothelial cells directly bioprinted within microfibrous hydrogel scaffolds gradually migrated towards the peripheries of the microfibers to form a layer of confluent endothelium...
December 2016: Biomaterials
Soonchul Lee, Jia Shen, Hsin Chuan Pan, Swati Shrestha, Greg Asatrian, Alan Nguyen, Carolyn Meyers, Vi Nguyen, Min Lee, Chia Soo, Kang Ting, Aaron W James
Hedgehog (Hh) signaling positively regulates both endochondral and intramembranous ossification. Use of small molecules for tissue engineering applications pose several advantages. Here, we examined whether use of an acellular scaffold treated with the small molecule Smoothened agonist (SAG) could aid in critical size mouse calvarial defect repair. First, we verified the pro-osteogenic effect of SAG in vitro, using primary neonatal mouse calvarial cells (NMCCs). Next, a 4 mm non-healing defect was created in the mid-parietal bone of ten week old CD-1 mice...
October 5, 2016: Tissue Engineering. Part A
Margot Den Hondt, Bart M Vanaudenaerde, Erik K Verbeken, Jan J Vranckx
BACKGROUND: Successful trachea transplantation comprises the use of biocompatible constructs with little immune-reactivity, submucosal revascularization and creation of an epithelial covering. Allogenic chondrocytes might be protected from an overt immune-response due to physical isolation. Our aim was to evaluate in-vivo biocompatibility of allotracheae, stripped of their highly-immunogenic inner lining. Secondly, we established whether these constructs might serve as suitable scaffolds for autologous epithelial grafting...
October 4, 2016: Acta Chirurgica Belgica
Joong-Hyun Kim, Tae-Hyun Kim, Min Sil Kang, Hae-Won Kim
Vascularization is a key issue for the success of tissue engineering to repair damaged tissue. In this study, we report a composite scaffold delivering angiogenic factor for this purpose. Vascular endothelial growth factor (VEGF) was loaded on mesoporous silica nanoparticle (MSN), which was then incorporated within a type I collagen sponge, to produce collagen/MSN/VEGF (CMV) scaffold. The CMV composite scaffold could release VEGF sustainably over the test period of 28 days. The release of VEGF improved the cell proliferation...
2016: BioMed Research International
Aida Llucià-Valldeperas, Carolina Soler-Botija, Carolina Gálvez-Montón, Santiago Roura, Cristina Prat-Vidal, Isaac Perea-Gil, Benjamin Sanchez, Ramon Bragos, Gordana Vunjak-Novakovic, Antoni Bayes-Genis
: : Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the viability of a tissue-engineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time...
September 29, 2016: Stem Cells Translational Medicine
Alexander Moncion, Keith J Arlotta, Eric G O'Neill, Melissa Lin, Lily A Mohr, Renny T Franceschi, Oliver D Kripfgans, Andrew J Putnam, Mario L Fabiilli
: Spatiotemporally controlled release of growth factors (GFs) is critical for regenerative processes such as angiogenesis. A common strategy is to encapsulate the GF within hydrogels, with release being controlled via diffusion and/or gel degradation (i.e., hydrolysis and/or proteolysis). However, simple encapsulation strategies do not provide spatial or temporal control of GF delivery, especially non-invasive, on-demand controlled release post implantation. We previously demonstrated that fibrin hydrogels, which are widely used in tissue engineering and GF delivery applications, can be doped with perfluorocarbon emulsion, thus yielding an acoustically responsive scaffold (ARS) that can be modulated with focused ultrasound, specifically via a mechanism termed acoustic droplet vaporization...
September 27, 2016: Acta Biomaterialia
Meredith A Roberts, Surya S Kotha, Kiet T Phong, Ying Zheng
In vitro platforms to study endothelial cells and vascular biology are largely limited to 2D endothelial cell culture, flow chambers with polymer or glass based substrates, and hydrogel-based tube formation assays. These assays, while informative, do not recapitulate lumen geometry, proper extracellular matrix, and multi-cellular proximity, which play key roles in modulating vascular function. This manuscript describes an injection molding method to generate engineered vessels with diameters on the order of 100 µm...
2016: Journal of Visualized Experiments: JoVE
Satyabrat Gogoi, Somnath Maji, Debasish Mishra, K Sanjana P Devi, Tapas Kumar Maiti, Niranjan Karak
The present study delves into a combined bio-nano-macromolecular approach for bone tissue engineering. This approach relies on the properties of an ideal scaffold material imbued with all the chemical premises required for fostering cellular growth and differentiation. A tannic acid based water dispersible hyperbranched polyurethane is fabricated with bio-nanohybrids of carbon dot and four different peptides (viz. SVVYGLR, PRGDSGYRGDS, IPP, and CGGKVGKACCVPTKLSPISVLYK) to impart target specific in vivo bone healing ability...
September 29, 2016: Macromolecular Bioscience
Hiromasa Yamashita, Hisae Aoki, Kenkichi Tanioka, Toshiyuki Mori, Toshio Chiba
BACKGROUND: We have started clinical application of 8K ultra-high definition (UHD; 7680 × 4320 pixels) imaging technology, which is a 16-fold higher resolution than the current 2K high-definition (HD; 1920 × 1080 pixels) technology, to an endoscope for advanced laparoscopic surgery. RESULTS: Based on preliminary testing experience and with subsequent technical and system improvements, we then proceeded to perform two cases of cholecystectomy and were able to achieve clinical success with an 8K UHD endoscopic system, which consisted of an 8K camera, a 30-degrees angled rigid endoscope with a lens adapter, a pair of 300-W xenon light sources, an 85-inch 8K LCD and an 8K video recorder...
2016: SpringerPlus
Tonia C Rothuizen, Raymond Kemp, Jacques M G J Duijs, Hetty C De Boer, Roel Bijkerk, Eric P Van der Veer, Lorenzo Moroni, Anton Jan Van Zonneveld, Anthony S Weiss, Ton J Rabelink, Joris I Rotmans
BACKGROUND: Elastin, critical for its structural and regulatory functions, is a missing link in vascular tissue engineering. Several elastin-inducting compounds have previously been reported, but their relative efficiency in promoting elastogenesis by adult arterial and venous vascular smooth muscle cells (VSMCs) and fibroblasts, four main vascular and elastogenic cells, has not been described. In addition to elasto-inductive substances, microRNA-29a was recently established as a potent post-transcriptional inhibitor of elastogenesis...
September 7, 2016: Tissue Engineering. Part C, Methods
Farrokh Sharifi, Bhavika B Patel, Adam K Dzuilko, Reza Montazami, Donald S Sakaguchi, Nastaran Hashemi
Fibrous scaffolds have shown promise in tissue engineering due to their ability to improve cell alignment and migration. In this paper, poly(ε-caprolactone) (PCL) fibers are fabricated in different sizes using a microfluidic platform. By using this approach, we demonstrated considerable flexibility in ability to control the size of the fibers. It was shown that the average diameter of the fibers was obtained in the range of 2.6-36.5 μm by selecting the PCL solution flow rate from 1 to 5 μL min(-1) and the sheath flow rate from 20 to 400 μL min(-1) in the microfluidic channel...
October 10, 2016: Biomacromolecules
Takehito Momose, Hirofumi Miyaji, Akihito Kato, Kosuke Ogawa, Takashi Yoshida, Erika Nishida, Syusuke Murakami, Yuta Kosen, Tsutomu Sugaya, Masamitsu Kawanami
OBJECTIVE: Collagen hydrogel scaffold exhibits bio-safe properties and facilitates periodontal wound healing. However, regenerated tissue volume is insufficient. Fibroblast growth factor-2 (FGF2) up-regulates cell behaviors and subsequent wound healing. We evaluated whether periodontal wound healing is promoted by application of collagen hydrogel scaffold in combination with FGF2 in furcation defects in beagle dogs. METHODS: Collagen hydrogel was fabricated from bovine type I collagen with an ascorbate-copper ion cross-linking system...
2016: Open Dentistry Journal
Keita Kinoshita, Masaki Iwase, Masumi Yamada, Yuya Yajima, Minoru Seki
Vascular tissues fabricated in vitro are useful tools for studying blood vessel-related cellular physiologies and for constructing relatively large 3D tissues. Here we propose an efficient strategy for fabricating vascular tissue models with multilayered, branched, and thick structures through the in situ hydrogel formation in fluidic channels. First, an aqueous solution of RGD-alginate containing smooth muscle cells (SMCs) was introduced into channel structures made of agarose hydrogel, forming a cell-embedding Ca-alginate hydrogel layer with a thickness of several hundred micrometers on the channel surface because of the Ca2+ ions diffused from the agarose hydrogel matrix...
August 31, 2016: Biotechnology Journal
Inés Moreno-Jiménez, Gry Hulsart-Billstrom, Stuart A Lanham, Agnieszka A Janeczek, Nasia Kontouli, Janos M Kanczler, Nicholas D Evans, Richard Oc Oreffo
Biomaterial development for tissue engineering applications is rapidly increasing but necessitates efficacy and safety testing prior to clinical application. Current in vitro and in vivo models hold a number of limitations, including expense, lack of correlation between animal models and human outcomes and the need to perform invasive procedures on animals; hence requiring new predictive screening methods. In the present study we tested the hypothesis that the chick embryo chorioallantoic membrane (CAM) can be used as a bioreactor to culture and study the regeneration of human living bone...
2016: Scientific Reports
Timon Hussain, Manuela Schneider, Burkhard Summer, Sebastian Strieth
BACKGROUND: Key factors for successful porous polyethylene (PPE) implantation are rapid vascularization and low inflammatory response. Dermal fibroblasts produce a variety of pro-angiogenic and immunmodulatory factors. OBJECTIVE: The aim of this tissue engineering study was to investigate whether coating PPE implants with dermal fibroblasts in vitro is sustainable in vivo and whether the kinetics of blood vessel ingrowth and immunological responses are hereby affected...
August 12, 2016: Bio-medical Materials and Engineering
Luuk R Versteegden, Henk R Hoogenkamp, Roger M Lomme, Harry van Goor, Dorien M Tiemessen, Paul J Geutjes, Egbert Oosterwijk, Wout F Feitz, Theo G Hafmans, Nico Verdonschot, Willeke F Daamen, Toin H van Kuppevelt
UNLABELLED: Type I collagen is widely applied as a biomaterial for tissue regeneration. In the extracellular matrix, collagen provides strength but not elasticity under large deformations, a characteristic crucial for dynamic organs and generally imparted by elastic fibers. In this study, a methodology is described to induce elastic-like characteristics in a scaffold consisting of solely type I collagen. Tubular scaffolds are prepared from collagen fibrils by a casting, molding, freezing and lyophilization process...
October 15, 2016: Acta Biomaterialia
Ai Ozaki, Yoshinori Arisaka, Naoya Takeda
Shear stress caused by fluid flow is known to promote tissue development from cells in vivo. Therefore, perfusion cultures have been studied to investigate the mechanisms involved and to fabricate engineered tissues in vitro, particularly those that include blood vessels. Microfluidic devices, which function with fine machinery of chambers and microsyringes for fluid flow and have small culture areas, are conventionally used for perfusion culture. In contrast, we have developed a self-driven perfusion culture system by using a paper-based double-layered scaffold as the fundamental component...
2016: Biofabrication
Kai Hsia, Chao-Ling Yao, Wei-Min Chen, Jian-Haw Chen, Hsinyu Lee, Jen-Her Lu
The increasing morbidity of cardiovascular diseases in modern society has made it crucial to develop a small-caliber blood vessel. In the absence of appropriate autologous vascular grafts, an alternative prosthesis must be constructed for cardiovascular disease patients. The aim of this article is to describe the advances in making cell-seeded cardiovascular prostheses. It also discusses the combinations of types of scaffolds and cells, especially autologous stem cells, which are suitable for application in tissue-engineered vessels with the favorable properties of mechanical strength, antithrombogenicity, biocompliance, anti-inflammation, fatigue resistance and long-term durability...
August 23, 2016: Cells, Tissues, Organs
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