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tissue engineered blood vessel

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https://www.readbyqxmd.com/read/28642016/electrospun-vascular-scaffold-for-cellularized-small-diameter-blood-vessels-a-preclinical-large-animal-study
#1
Young Min Ju, Hyunhee Ahn, Juan Arenas-Herrera, Cheil Kim, Mehran Abolbashari, Anthony Atala, James J Yoo, Sang Jin Lee
The strategy of vascular tissue engineering is to create a vascular substitute by combining autologous vascular cells with a tubular-shaped biodegradable scaffold. We have previously developed a novel electrospun bilayered vascular scaffold that provides proper biological and biomechanical properties as well as structural configuration. In this study, we investigated the clinical feasibility of a cellularized vascular scaffold in a preclinical large animal model. We fabricated the cellularized vascular construct with autologous endothelial progenitor cell (EPC)-derived endothelial cells (ECs) and smooth muscle cells (SMCs) followed by a pulsatile bioreactor preconditioning...
June 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28629021/novel-hipsc-based-tri-culture-for-pre-vascularization-of-calcium-phosphate-scaffold-to-enhance-bone-and-vessel-formation
#2
Chi Zhang, Kevin Hu, Xian Liu, Mark A Reynolds, Chongyun Bao, Ping Wang, Liang Zhao, Hockin H K Xu
Vascularization of tissue-engineered bone is a critical step in maintaining cell viability and advancing cell performance in vivo. In this study, a novel tri-culture system was developed to elicit pre-vascularization of calcium phosphate cement (CPC) scaffold in which human induced pluripotent stem cell-derived mesenchymal stem cells (hiPSMSCs) were seeded together with human umbilical vein endothelial cells (HUVECs) and pericytes. In a two-step methodology design, we first performed osteoinduction of the seeded hiPSMSCs on the CPC scaffold and then incorporated HUVECs and pericytes to the hiPSMSC-colonized CPC scaffold under a favorable culturing condition, with an objective to form a stable and functional capillary-like vascular network that sustained the engineered osseous tissue...
October 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28614918/the-significance-of-sdf-1alpha-cxcr4-axis-in-in-vivo-angiogenic-ability-of-human-periodontal-ligament-stem-cells
#3
Yoon-Kyung Bae, Gee-Hye Kim, Jae Cheoun Lee, Byoung-Moo Seo, Kyeung-Min Joo, Gene Lee, Hyun Nam
Periodontal ligament stem cells (PDLSCs) are multipotent stem cells derived from periodontium and have mesenchymal stem cell (MSC)-like characteristics. Recently, the perivascular region was recognized as the developmental origin of MSCs, which suggests the in vivo angiogenic potential of PDLSCs. In this study, we investigated whether PDLSCs could be a potential source of perivascular cells, which could contribute to in vivo angiogenesis. PDLSCs exhibited typical MSC-like characteristics such as the expression pattern of surface markers (CD29, CD44, CD73, and CD105) and differentiation potentials (osteogenic and adipogenic differentiation)...
June 14, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28607933/optimal-branching-structure-of-fluidic-networks-with-permeable-walls
#4
Vinicius R Pepe, Luiz A O Rocha, Antonio F Miguel
Biological and engineering studies of Hess-Murray's law are focused on assemblies of tubes with impermeable walls. Blood vessels and airways have permeable walls to allow the exchange of fluid and other dissolved substances with tissues. Should Hess-Murray's law hold for bifurcating systems in which the walls of the vessels are permeable to fluid? This paper investigates the fluid flow in a porous-walled T-shaped assembly of vessels. Fluid flow in this branching flow structure is studied numerically to predict the configuration that provides greater access to the flow...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28599055/delivered-adipose-derived-stromal-cells-improve-host-derived-adipose-tissue-formation-in-composite-constructs-in-vivo
#5
Katharina Storck, Reyk Fischer, Maria Buchberger, Bernhard Haller, Sybille Regn
OBJECTIVES/HYPOTHESIS: Adipose tissue engineering aims to provide functional tissue surrogates for the restoration of soft tissue defects and contour deformities in the face. Many studies involve the delivery of cells; however, the impact and the exact role of the implanted cells is not yet fully elucidated. STUDY DESIGN: Animal research. METHODS: In this study, we used a mouse model for the development of volume-stable adipose tissue using polyurethane scaffolds combined with a long-term stable fibrin gel and adipose-derived stromal cells to investigate the influence of cell delivery on tissue development...
June 9, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28592214/multinozzle-multichannel-temperature-deposition-system-for-construction-of-a-blood-vessel
#6
Huanbao Liu, Huixing Zhou, Haiming Lan, Fu Liu, Xuhan Wang
3D bioprinting is an emerging technology that drives us to construct the complicated tissues and organs consisting of various materials and cells, which has been in widespread use in tissue engineering and organ regeneration. However, the protection and accurate distribution of cells are the most urgent problems to achieve tissue and organ reconstruction. In this article, a multinozzle multichannel temperature deposition and manufacturing (MTDM) system is proposed to fabricate a blood vessel with heterogeneous materials and gradient hierarchical porous structures, which enables not only the reconstruction of a blood vessel with an accurate 3D model structure but also the capacity to distribute bioactive materials such as growth factors, nutrient substance, and so on...
June 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28585207/endothelial-dysfunction-in-obesity
#7
Atilla Engin
Chronic inflammatory state in obesity causes dysregulation of the endocrine and paracrine actions of adipocyte-derived factors, which disrupt vascular homeostasis and contribute to endothelial vasodilator dysfunction and subsequent hypertension. While normal healthy perivascular adipose tissue (PVAT) ensures the dilation of blood vessels, obesity-associated PVAT leads to a change in profile of the released adipo-cytokines, resulting in a decreased vasorelaxing effect. Adipose tissue inflammation, nitric oxide (NO)-bioavailability, insulin resistance and oxidized low-density lipoprotein (oxLDL) are main participating factors in endothelial dysfunction of obesity...
2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28583167/engineering-biomimetic-periosteum-with-%C3%AE-tcp-scaffolds-to-promote-bone-formation-in-calvarial-defects-of-rats
#8
Dan Zhang, Peng Gao, Qin Li, Jinda Li, Xiaojuan Li, Xiaoning Liu, Yunqing Kang, Liling Ren
BACKGROUND: There is a critical need for the management of large bone defects. The purpose of this study was to engineer a biomimetic periosteum and to combine this with a macroporous β-tricalcium phosphate (β-TCP) scaffold for bone tissue regeneration. METHODS: Rat bone marrow-derived mesenchymal stem cells (rBMSCs) were harvested and cultured in different culture media to form undifferentiated rBMSC sheets (undifferentiated medium (UM)) and osteogenic cell sheets (osteogenic medium (OM))...
June 5, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28582613/flow-induced-shape-reconfiguration-phase-separation-and-rupture-of-bio-inspired-vesicles
#9
Xiaolei Chu, Xiang Yu, Joseph Greenstein, Fikret Aydin, Geetartha Uppaladadium, Meenakshi Dutt
The structural integrity of red blood cells and drug delivery carriers through blood vessels is dependent upon their ability to adapt their shape during their transportation. Our goal is to examine the role of the composition of bio-inspired multicomponent and hairy vesicles on their shape during their transport through in a channel. Through the dissipative particle dynamics simulation technique, we apply Poiseuille flow in a cylindrical channel. We investigate the effect of flow conditions and concentration of key molecular components on the shape, phase separation, and structural integrity of the bio-inspired multicomponent and hairy vesicles...
June 12, 2017: ACS Nano
https://www.readbyqxmd.com/read/28575984/chitosan-%C3%AE-poly-glutamic-acid-scaffolds-with-surface-modified-albumin-elastin-and-poly-l-lysine-for-cartilage-tissue-engineering
#10
Yung-Chih Kuo, Hao-Fu Ku, Rajendiran Rajesh
Cartilage has limited ability to self-repair due to the absence of blood vessels and nerves. The application of biomaterial scaffolds using biomimetic extracellular matrix (ECM)-related polymers has become an effective approach to production of engineered cartilage. Chitosan/γ-poly(glutamic acid) (γ-PGA) scaffolds with different mass ratios were prepared using genipin as a cross-linker and a freeze-drying method, and their surfaces were modified with elastin, human serum albumin (HSA) and poly-l-lysine (PLL)...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28571106/sdf-1%C3%AE-peptide-tethered-polyester-facilitates-tissue-repair-by-endogenous-cell-mobilization-and-recruitment
#11
Muhammad Shafiq, Deling Kong, Soo Hyun Kim
The design of bioactive scaffolds that can invoke host's own regenerative capabilities and facilitate endogenous tissue repair hold great promise. This study aims to evaluate the potential of stromal cell-derived factor 1 alpha (SDF-1α)-derived peptide and heparin tethered poly(L-lactide-co-ε-caprolactone) (PLCL) copolymers for blood vessel regeneration applications. Amino acid analysis and toluidine blue assays confirm successful conjugation of SDF-1α peptide and heparin with the PLCL copolymers. Assessment of biocompatibility after subcutaneous implantation in rats discloses higher cell infiltration in SDF-1α peptide (SDF-1 group) or SDF-1 peptide and heparin (SDF-1/heparin group) than the control group...
June 1, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28570946/microfabricated-blood-vessels-undergo-neoangiogenesis
#12
Kyle A DiVito, Michael A Daniele, Steven A Roberts, Frances S Ligler, André A Adams
The greatest ambition and promise of tissue engineering is to manufacture human organs. Before "made-to-measure" tissues can become a reality [1-3], however, three-dimensional tissues must be reconstructed and characterized. The current inability to manufacture operational vasculature has limited the growth of engineered tissues. Here, free-standing, small diameter blood vessels with organized cell layers that recapitulate normal biological functionality are fabricated using microfluidic technology. Over time in culture, the endothelial cells form a monolayer on the luminal wall and remodel the scaffold with human extracellular matrix proteins...
May 19, 2017: Biomaterials
https://www.readbyqxmd.com/read/28569086/estimation-of-the-physiological-mechanical-conditioning-in-vascular-tissue-engineering-by-a-predictive-fluid-structure-interaction-approach
#13
Claudia Tresoldi, Elena Bianchi, Alessandro Filippo Pellegata, Gabriele Dubini, Sara Mantero
The in vitro replication of physiological mechanical conditioning through bioreactors plays a crucial role in the development of functional Small-Caliber Tissue-Engineered Blood Vessels. An in silico scaffold-specific model under pulsatile perfusion provided by a bioreactor was implemented using a fluid-structure interaction (FSI) approach for viscoelastic tubular scaffolds (e.g. decellularized swine arteries, DSA). Results of working pressures, circumferential deformations, and wall shear stress on DSA fell within the desired physiological range and indicated the ability of this model to correctly predict the mechanical conditioning acting on the cells-scaffold system...
August 2017: Computer Methods in Biomechanics and Biomedical Engineering
https://www.readbyqxmd.com/read/28557870/in-situ-delivery-of-fibrin-based-hydrogels-prevents-contraction-and-reduces-inflammation
#14
David M Burmeister, Daniel C Roy, Sandra C Becerra, Shanmugasundaram Natesan, Robert J Christy
While early excision and grafting has revolutionized burn wound care, autologous split-thickness skin grafts are sometimes unavailable. Tissue-engineered skin substitutes have generated great interest but have proven inadequate. Therefore, the development of novel biomaterials to replace/augment skin grafting could improve burn patient outcomes. Herein, we establish the effects of debridement on deep-partial thickness burns and subsequently examine the effects of 3 different hydrogels on healing. Burns were created on the dorsum of pigs and 4 days after, the eschar was either left intact or debrided for treatment with collagen, PEGylated fibrinogen (PEG-fibrin) or PEGylated autologous platelet-free plasma hydrogels...
May 23, 2017: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/28556610/vegf-release-from-a-polymeric-nanofiber-scaffold-for-improved-angiogenesis
#15
Hadar Zigdon-Giladi, Alaa Khutaba, Rina Elimelech, Eli E Machtei, Samer Srouji
Angiogenesis plays a pivotal role in tissue engineering and regenerative medicine. This study aimed to develop an electrospun fiber scaffold that supports release of recombinant human vascular endothelial growth factor (rhVEGF) to enhance angiogenesis. Scaffolds composed of core-shell fibers were fabricated using co-electrospinning. The core solution was composed of polyethylene oxide and mixed with rhVEGF. The shell solution was composed of polycarpolactone, with 0.25, 1, and 3% of polyethylene glycol (PEG) to manipulate pore size on the shell...
May 27, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28547766/local-release-of-vegf-using-fiber-mats-enables-effective-transplantation-of-layered-cardiomyocyte-sheets
#16
Kenichi Nagase, Yuhei Nagumo, Miri Kim, Hee-Jung Kim, Hei-Won Kyung, Hye-Jin Chung, Hidekazu Sekine, Tatsuya Shimizu, Hideko Kanazawa, Teruo Okano, Seung-Jin Lee, Masayuki Yamato
Cell sheet transplantation is a key tissue engineering technology. A vascular endothelial growth factor (VEGF)-releasing fiber mat is developed for the transplantation of multilayered cardiomyocyte sheets. Poly(vinyl alcohol) fiber mats bearing poly(lactic-co-glycolic acid) nanoparticles that incorporate VEGF are fabricated using electrospinning and electrospray methods. Six-layered cardiomyocyte sheets are transplanted with a VEGF-releasing mat into athymic rats. After two weeks, these sheets produce thicker cardiomyocyte layers compared with controls lacking a VEGF-releasing mat, and incorporate larger-diameter blood vessels containing erythrocytes...
May 26, 2017: Macromolecular Bioscience
https://www.readbyqxmd.com/read/28546952/time-dependency-of-morphological-remodeling-of-endothelial-cells-in-response-to-substrate-stiffness
#17
Zahra Goli-Malekabadi, Mohammad Tafazzoli-Shadpour, Ali Tamayol, Ehsan Seyedjafari
Introduction: Substrate stiffness regulates cellular behavior as cells experience different stiffness values of tissues in the body. For example, endothelial cells (ECs) covering the inner layer of blood vessels are exposed to different stiffness values due to various pathologic and physiologic conditions. Despite numerous studies, cells by time span sense mechanical properties of the substrate, but the response is not well understood. We hypothesized that time is a major determinant influencing the behavior of cells seeded on substrates of varying stiffness...
2017: BioImpacts: BI
https://www.readbyqxmd.com/read/28524375/bioprinted-osteogenic-and-vasculogenic-patterns-for-engineering-3d-bone-tissue
#18
Batzaya Byambaa, Nasim Annabi, Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Weitao Jia, Mehdi Kazemzadeh-Narbat, Su Ryon Shin, Ali Tamayol, Ali Khademhosseini
Fabricating 3D large-scale bone tissue constructs with functional vasculature has been a particular challenge in engineering tissues suitable for repairing large bone defects. To address this challenge, an extrusion-based direct-writing bioprinting strategy is utilized to fabricate microstructured bone-like tissue constructs containing a perfusable vascular lumen. The bioprinted constructs are used as biomimetic in vitro matrices to co-culture human umbilical vein endothelial cells and bone marrow derived human mesenchymal stem cells in a naturally derived hydrogel...
May 19, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28513058/pro-myogenic-and-low-oxygen-culture-increases-expression-of-contractile-smooth-muscle-markers-in-human-fibroblasts
#19
Matija Veber, David Dolivo, Marsha Rolle, Tanja Dominko
Smooth muscle cells are essential for tissue engineering strategies to fabricate organs such as blood vessels, esophagus, and bladder, and to create disease models of these systems. In order for such therapies and models to be feasible, smooth muscle cells must be sourced effectively to enable production of large numbers of functional cells. In vitro, smooth muscle cells divide slowly and demonstrate short proliferative lifespans compared to other types of cells including stem cells and fibroblasts, limiting the number of cells that can be derived from expansion in culture of a primary isolation...
May 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28495397/safety-profile-and-long-term-engraftment-of-human-cd31-blood-progenitors-in-bone-tissue-engineering
#20
Hadar Zigdon-Giladi, Rina Elimelech, Gal Michaeli-Geller, Utai Rudich, Eli E Machtei
BACKGROUND: Endothelial progenitor cells (EPCs) participate in angiogenesis and induce favorable micro-environments for tissue regeneration. The efficacy of EPCs in regenerative medicine is extensively studied; however, their safety profile remains unknown. Therefore, our aims were to evaluate the safety profile of human peripheral blood-derived EPCs (hEPCs) and to assess the long-term efficacy of hEPCs in bone tissue engineering. METHODS: hEPCs were isolated from peripheral blood, cultured and characterized...
July 2017: Cytotherapy
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