keyword
MENU ▼
Read by QxMD icon Read
search

scheffer c g Tseng

keyword
https://www.readbyqxmd.com/read/27739458/senescence-mediated-by-p16-ink4a-impedes-reprogramming-of-human-corneal-endothelial-cells-into-neural-crest-progenitors
#1
Wen-Juan Lu, Scheffer C G Tseng, Shuangling Chen, Sean Tighe, Yuan Zhang, Xin Liu, Szu-Yu Chen, Chen-Wei Su, Ying-Ting Zhu
Human corneal endothelial cells (HCECs) have limited proliferative capacity due to "contact-inhibition" at G1 phase. Such contact-inhibition can be delayed from Day 21 to Day 42 by switching EGF-containing SHEM to LIF/bFGF-containing MESCM through transient activation of LIF-JAK1-STAT3 signaling that delays eventual nuclear translocation of p16(INK4a). Using the latter system, we have reported a novel tissue engineering technique by implementing 5 weekly knockdowns with p120 catenin (p120) and Kaiso siRNAs since Day 7 to achieve effective expansion of HCEC monolayers to a transplantable size with a normal HCEC density, through reprogramming of HCECs into neural crest progenitors by activating p120-Kaiso-RhoA-ROCK-canonical BMP signaling...
October 14, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27621952/neurological-outcomes-after-human-umbilical-cord-patch-for-in-utero-spina-bifida-repair-in-a-sheep-model
#2
Ramesha Papanna, Lovepreet K Mann, Saul Snowise, Yisel Morales, Sanjay P Prabhu, Scheffer C G Tseng, Raymond Grill, Stephen Fletcher, Kenneth J Moise
OBJECTIVES: The objective of our study was to test the hypothesis that in utero repair of surgically created spina bifida in a sheep model using cryopreserved human umbilical cord (HUC) patch improves neurological outcome. METHODS: Spina bifida with myelotomy was surgically created in timed pregnant ewes at gestational day (GD) 75. The fetuses were randomly assigned to unrepaired versus HUC and treated at GD 95 and then delivered at GD 140. Neurological evaluation was performed using the Texas Spinal Cord Injury Scale (TSCIS), bladder control using ultrasound, and the hindbrain herniation...
July 2016: American Journal of Perinatology Reports
https://www.readbyqxmd.com/read/27400004/cryopreserved-human-umbilical-cord-for-in-utero-myeloschisis-repair
#3
Ramesha Papanna, Stephen Fletcher, Kenneth J Moise, Lovepreet K Mann, Scheffer C G Tseng
BACKGROUND: There is an urgent need is for a regenerative patch for in utero repair of spina bifida in situations in which primary closure is difficult. CASES: We present two cases of large myeloschisis defects that underwent in utero spina bifida repair at midgestation with closure of the skin defect by using a cryopreserved human umbilical cord patch. The pregnancies were uncomplicated, and the births occurred at 37 weeks by planned cesarean delivery. The repair sites were intact with no evidence of cerebrospinal fluid leakage, with skin regenerated after delivery over a period of 3-4 weeks...
August 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27226933/morselized-amniotic-membrane-tissue-for-refractory-corneal-epithelial-defects-in-cicatricial-ocular-surface-diseases
#4
Anny M S Cheng, Lorraine Chua, Victoria Casas, Scheffer C G Tseng
PURPOSE: To evaluate the clinical efficacy of morselized amniotic membrane and umbilical cord tissue (MAU) in treating refractory corneal epithelial defect in ocular cicatricial diseases. METHODS: Retrospective review of four patients with ocular cicatricial diseases treated with topical MAU for corneal epithelial defects refractory to conventional treatments including topical lubricants, autologous serum, bandage contact lens, and tarsorraphy. Their symptoms, corneal staining, conjunctival inflammation, and visual acuity were compared before and after treatment...
May 2016: Translational Vision Science & Technology
https://www.readbyqxmd.com/read/27116665/hc-ha-ptx3-purified-from-amniotic-membrane-as-novel-regenerative-matrix-insight-into-relationship-between-inflammation-and-regeneration
#5
Scheffer C G Tseng
PURPOSE: Human limbal palisade of Vogt is an ideal model for studying and practicing regenerative medicine due to their accessibility. Nonresolving inflammation is a common manifestation of limbal stem cell deficiency, which is the major cause of corneal blindness, and presents as a threat to the success of transplanted limbal epithelial stem cells. Clinical studies have shown that the efficacy of transplantation of limbal epithelial stem cells can be augmented by transplantation of cryopreserved human amniotic membrane (AM), which exerts anti-inflammatory, antiscarring, and antiangiogenic action to promote wound healing...
April 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27063525/sutureless-cryopreserved-amniotic-membrane-graft-and-wound-healing-after%C3%A2-photorefractive-keratectomy
#6
Anton Vlasov, Rose K Sia, Denise S Ryan, Michael J Mines, Richard D Stutzman, Bruce A Rivers, Scheffer C G Tseng, Kraig S Bower
PURPOSE: To evaluate the effect of sutureless cryopreserved amniotic membrane (Prokera) on corneal wound healing after photorefractive keratectomy (PRK). SETTING: Center for Refractive Surgery, Walter Reed Army Medical Center, Washington, DC, USA. DESIGN: Prospective nonrandomized control trial. METHODS: Patients had PRK for myopia with or without astigmatism. A 20% ethanol solution was used to create a standard 9.0 mm epithelial defect followed by photoablation with the Allegretto Wave Eye-Q 400 Hz laser...
March 2016: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/26890668/restoration-of-fornix-tear-reservoir-in-conjunctivochalasis-with-fornix-reconstruction
#7
Anny M S Cheng, Han Y Yin, Rendian Chen, Sean Tighe, Hosam Sheha, Dandan Zhao, Victoria Casas, Scheffer C G Tseng
PURPOSE: To determine whether conjunctivochalasis (CCh) obliterates the fornix tear reservoir and to discern whether there is concomitant aqueous tear deficiency (ATD) dry eye. METHODS: Retrospective review of 18 eyes of 12 patients with CCh and ATD (CCh + ATD) and 18 eyes of 13 patients with CCh without ATD (CCh - ATD). Changes were compared before and after fornix reconstruction regarding symptoms, basal tear volumes, use of medications, conjunctival inflammation, and corneal staining...
June 2016: Cornea
https://www.readbyqxmd.com/read/26882981/acute-and-chronic-ophthalmic-involvement-in-stevens-johnson-syndrome-toxic-epidermal-necrolysis-a-comprehensive-review-and-guide-to-therapy-ii-ophthalmic-disease
#8
REVIEW
Sahar Kohanim, Sotiria Palioura, Hajirah N Saeed, Esen K Akpek, Guillermo Amescua, Sayan Basu, Preston H Blomquist, Charles S Bouchard, John K Dart, Xiaowu Gai, José A P Gomes, Darren G Gregory, Geetha Iyer, Deborah S Jacobs, Anthony J Johnson, Shigeru Kinoshita, Iason S Mantagos, Jodhbir S Mehta, Victor L Perez, Stephen C Pflugfelder, Virender S Sangwan, Kimberly C Sippel, Chie Sotozono, Bhaskar Srinivasan, Donald T H Tan, Radhika Tandon, Scheffer C G Tseng, Mayumi Ueta, James Chodosh
Our purpose is to comprehensively review the state of the art with regard to Stevens- Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), with particular attention to improving the management of associated ocular surface complications. SJS and TEN are two ends of a spectrum of immune-mediated disease, characterized in the acute phase by a febrile illness followed by skin and mucous membrane necrosis and detachment. Part I of this review focused on the systemic aspects of SJS/TEN and was published in the January 2016 issue of this journal...
April 2016: Ocular Surface
https://www.readbyqxmd.com/read/26549248/stevens-johnson-syndrome-toxic-epidermal-necrolysis-a-comprehensive-review-and-guide-to-therapy-i-systemic-disease
#9
REVIEW
Sahar Kohanim, Sotiria Palioura, Hajirah N Saeed, Esen K Akpek, Guillermo Amescua, Sayan Basu, Preston H Blomquist, Charles S Bouchard, John K Dart, Xiaowu Gai, José A P Gomes, Darren G Gregory, Geetha Iyer, Deborah S Jacobs, Anthony J Johnson, Shigeru Kinoshita, Iason S Mantagos, Jodhbir S Mehta, Victor L Perez, Stephen C Pflugfelder, Virender S Sangwan, Kimberly C Sippel, Chie Sotozono, Bhaskar Srinivasan, Donald T H Tan, Radhika Tandon, Scheffer C G Tseng, Mayumi Ueta, James Chodosh
The intent of this review is to comprehensively appraise the state of the art with regard to Stevens Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN), with particular attention to the ocular surface complications and their management. SJS and TEN represent two ends of a spectrum of immune-mediated, dermatobullous disease, characterized in the acute phase by a febrile illness followed by skin and mucous membrane necrosis and detachment. The widespread keratinocyte death seen in SJS/TEN is rapid and irreversible, and even with early and aggressive intervention, morbidity is severe and mortality not uncommon...
January 2016: Ocular Surface
https://www.readbyqxmd.com/read/26509105/engineering-of-human-corneal-endothelial-grafts
#10
Ying-Ting Zhu, Sean Tighe, Shuang-Ling Chen, Thomas John, Winston Y Kao, Scheffer C G Tseng
Human corneal endothelial cells (HCEC) play a pivotal role in maintaining corneal transparency. Unlike in other species, HCEC are notorious for their limited proliferative capacity in vivo after diseases, injury, aging, and surgery. Persistent HCEC dysfunction leads to sight-threatening bullous keratopathy with either an insufficient cell density or retrocorneal membrane due to endothelial-mesenchymal transition (EMT). Presently, the only solution to restore vision in eyes inflicted with bullous keratopathy or retrocorneal membrane relies upon transplantation of a cadaver human donor cornea containing a healthy corneal endothelium...
September 2015: Current Ophthalmology Reports
https://www.readbyqxmd.com/read/26489897/cryopreserved-human-umbilical-cord-patch-for-in-utero-spina-bifida-repair
#11
R Papanna, K J Moise, L K Mann, S Fletcher, R Schniederjan, M B Bhattacharjee, R J Stewart, S Kaur, S P Prabhu, S C G Tseng
OBJECTIVE: To identify a patch system to repair surgically created spina bifida in a sheep model for its efficacy in healing the skin defect, protecting the underlying spinal cord and reducing the Chiari II malformation. METHODS: Spina bifida was created surgically in 16 fetuses from eight timed-pregnant sheep at gestational age of 75 days. Two fetuses did not survive the procedure. Repeat hysterotomy was performed at 95 days' gestation to cover the defect with either biocellulose film with underwater adhesive (BCF-adhesive) (n = 7) or human umbilical cord with suture (HUC-suture) (n = 7)...
February 2016: Ultrasound in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/26387870/accelerated-restoration-of-ocular-surface-health-in-dry-eye-disease-by-self-retained-cryopreserved-amniotic-membrane
#12
Anny M S Cheng, Dandan Zhao, Rendian Chen, Han Y Yin, Sean Tighe, Hosam Sheha, Victoria Casas, Scheffer C G Tseng
PURPOSE: To evaluate the clinical efficacy of self-retained cryopreserved amniotic membrane in treating dry eye disease. METHODS: Retrospective review of 10 patients treated with self-retained cryopreserved amniotic membrane (PROKERA® Slim [PKS], Bio-Tissue, Miami, FL) for moderate-to-severe dry eye refractory to conventional maximal medical treatments. Patients' symptoms, use of medications, conjunctival inflammation, corneal staining, and visual acuity were compared before and after treatment...
January 2016: Ocular Surface
https://www.readbyqxmd.com/read/26148958/hc-ha-ptx3-purified-from-amniotic-membrane-promotes-bmp-signaling-in-limbal-niche-cells-to-maintain-quiescence-of-limbal-epithelial-progenitor-stem-cells
#13
Szu-Yu Chen, Bo Han, Ying-Ting Zhu, Megha Mahabole, Jie Huang, David C Beebe, Scheffer C G Tseng
To explore how limbal niche cells (LNCs) may control quiescence, self-renewal, and corneal epithelial lineage commitment/differentiation of limbal epithelial progenitor/stem cells (LEPCs), we have established an in vitro sphere assay by reunion between the two cell types in three-dimensional Matrigel. The resultant sphere exhibits inhibition of corneal epithelial lineage commitment/differentiation and marked clonal growth of LEPCs, of which the latter is correlated with activation of canonical Wnt signaling...
November 2015: Stem Cells
https://www.readbyqxmd.com/read/26093286/sealing-of-the-gap-between-the-conjunctiva-and-tenon-capsule-to-improve-symblepharon-surgery
#14
Dandan Zhao, Han Y Yin, Anny Cheng, Rendian Chen, Hosam Sheha, Scheffer C G Tseng
PURPOSE: To report the surgical outcome of "sealing the gap" in treating symblepharon caused by various etiologies other than recurrent pterygium. DESIGN: Retrospective, interventional case series. METHODS: Sixteen eyes of 14 patients with pathogenic symblepharon were consecutively operated by conjunctival recession, sealing the gap between recessed conjunctiva and Tenon capsule with a running 9-0 nylon suture, and covering of the bare sclera with amniotic membrane...
September 2015: American Journal of Ophthalmology
https://www.readbyqxmd.com/read/26058028/recent-advances-on-ocular-demodex-infestation
#15
REVIEW
Anny M S Cheng, Hosam Sheha, Scheffer C G Tseng
PURPOSE OF REVIEW: To summarize recent advances on ocular Demodex infestation. RECENT FINDINGS: Demodex infestation is a potential cause of ocular surface inflammation. The pathogenesis of Demodex in eliciting ocular surface inflammation has been further clarified. Cliradex is currently the treatment of choice, it comprises the most active ingredient of tea tree oil, that is terpinen-4-ol, which helps eradicate Demodex mites and reduce ocular surface inflammation...
July 2015: Current Opinion in Ophthalmology
https://www.readbyqxmd.com/read/25695744/lif-jak1-stat3-signaling-delays-contact-inhibition-of-human-corneal-endothelial-cells
#16
Xin Liu, Scheffer C G Tseng, Ming-Chang Zhang, Szu-Yu Chen, Sean Tighe, Wen-Juan Lu, Ying-Ting Zhu
Human corneal endothelial cells (HCECs) responsible for corneal transparency have limited proliferative capacity in vivo because of "contact-inhibition." This feature has hampered the ability to engineer HCECs for transplantation. Previously we have reported an in vitro model of HCECs in which contact inhibition was re-established at Day 21, even though cell junction and cell matrix interaction were not perturbed during isolation. Herein, we observe that such HCEC monolayers continue to expand and retain a normal phenotype for 2 more weeks if cultured in a leukemia inhibitory factor (LIF)-containing serum-free medium...
2015: Cell Cycle
https://www.readbyqxmd.com/read/25202030/activation-of-rhoa-rock-bmp-signaling-reprograms-adult-human-corneal-endothelial-cells
#17
Ying-Ting Zhu, Fu Li, Bo Han, Sean Tighe, Suzhen Zhang, Szu-Yu Chen, Xin Liu, Scheffer C G Tseng
Currently there are limited treatment options for corneal blindness caused by dysfunctional corneal endothelial cells. The primary treatment involves transplantation of healthy donor human corneal endothelial cells, but a global shortage of donor corneas necessitates other options. Conventional tissue approaches for corneal endothelial cells are based on EDTA-trypsin treatment and run the risk of irreversible endothelial mesenchymal transition by activating canonical Wingless-related integration site (Wnt) and TGF-β signaling...
September 15, 2014: Journal of Cell Biology
https://www.readbyqxmd.com/read/24994870/isolation-and-characterization-of-mesenchymal-progenitor-cells-from-human-orbital-adipose-tissue
#18
Szu-Yu Chen, Megha Mahabole, Elan Horesh, Sara Wester, Jeffrey L Goldberg, Scheffer C G Tseng
PURPOSE: Adipose-derived stem cells (ASCs) have gained importance due to their myriad potential clinical applications. We hypothesize that progenitor cells also exist besides those conventionally isolated from the stromal vascular fraction (SVF). METHOD: Central and medial orbital adipose tissues obtained from patients during eyelid surgery were digested with collagenase for 3 or 16 hours at 37°C with or without shaking. After centrifugation, the remaining cell pellet was resuspended and filtered to yield flow through in SVF and retained cells (RC) on the filter...
August 2014: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/24652286/constitutive-expression-of-pentraxin-3-ptx3-protein-by-human-amniotic-membrane-cells-leads-to-formation-of-the-heavy-chain-hc-hyaluronan-ha-ptx3-complex
#19
Suzhen Zhang, Ying-Ting Zhu, Szu-Yu Chen, Hua He, Scheffer C G Tseng
Heavy chain (HC)-hyaluronan (HA), a complex formed by the covalent linkage between HC1 from the inter-α-trypsin inhibitor (IαI) and HA, purified from the human amniotic membrane (AM), is responsible for the anti-inflammatory, antiscarring, and antiangiogenic actions of the AM. This HC-HA complex is produced by constitutive expression of TNF-stimulated gene 6 and endogenous production of IαI by AM cells. Pentraxin 3 (PTX3), a prototypic long pentraxin that plays a non-redundant role in innate immunity against selected pathogens, also helps stabilize HC-HA to ensure female fertility...
May 9, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24530980/integration-of-bmp-wnt-signaling-to-control-clonal-growth-of-limbal-epithelial-progenitor-cells-by-niche-cells
#20
Bo Han, Szu-Yu Chen, Ying-Ting Zhu, Scheffer C G Tseng
Both BMP and Wnt signaling control stem cells in bulge/dermal papilla, intestinal crypt, and bone marrow. To explore their roles in the limbal niche, which govern corneal epithelial homeostasis, we established an in vitro model of sphere growth by reunion between single limbal epithelial progenitor cells (LEPCs) and aggregates of limbal niche cells (LNCs) in 3D Matrigel. Compared to LEPCs alone, spheres formed by LEPC+LNC exhibited higher clonal growth and less corneal epithelial differentiation. Furthermore, pSmad1/5/8 was in the nucleus of LEPCs, but not LNCs, and correlated with upregulation of BMP1, BMP3, BMP4, all three BMP receptors, and BMP target genes...
March 2014: Stem Cell Research
keyword
keyword
68156
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"