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Vacuolar copper

Juanjuan Liu, Hongjing Zhao, Yu Wang, Yizhi Shao, Jinglun Li, Mingwei Xing
In this study, we sought to investigate the effects of sub-chronic exposure of arsenic (As) and copper (Cu) on oxidative stress, inflammatory response, and mitochondria and death receptor apoptosis pathways in chicken liver. Seventy-two 1-day-old male Hy-line chickens were treated with basal diet, 30 mg/kg arsenic trioxide (As2 O3 ), or/and 300 mg/kg copper sulfate (CuSO4 ) for 4, 8, and 12 weeks. Study revealed that exposure to As or/and Cu undermined the antioxidant function and increased lipid peroxidation...
March 22, 2018: Environmental Science and Pollution Research International
Zhong-Rui Xu, Shen-Wen Cai, Wu-Xing Huang, Rong-Xiang Liu, Zhi-Ting Xiong
Acid invertase activities in roots and young seeds of a metalliferous population (MP) of Rumex dentatus were previously observed to be significantly higher than those of a non-metalliferous population (NMP) under Cu stress. To date, no acid invertase gene has been cloned from R. dentatus. Here, we isolated four full-length cDNAs from the two populations of R. dentatus, presumably encoding cell wall (RdnCIN1 and RdmCIN1 from the NMP and MP, respectively) and vacuolar invertases (RdnVIN1 and RdmVIN1 from the NMP and MP, respectively)...
January 2018: Ecotoxicology and Environmental Safety
Hiroshi Nishikawa, Taiga Miyazaki, Hironobu Nakayama, Asuka Minematsu, Shunsuke Yamauchi, Kohei Yamashita, Takahiro Takazono, Shintaro Shimamura, Shigeki Nakamura, Koichi Izumikawa, Katsunori Yanagihara, Shigeru Kohno, Hiroshi Mukae
Vacuolar H(+)-ATPase (V-ATPase) is responsible for the acidification of eukaryotic intracellular compartments and plays an important role in oxidative stress response (OSR), but its molecular bases are largely unknown. Here, we investigated how V-ATPase is involved in the OSR by using a strain lacking VPH2, which encodes an assembly factor of V-ATPase, in the pathogenic fungus Candida glabrata The loss of Vph2 resulted in increased H2O2 sensitivity and intracellular reactive oxygen species (ROS) level independently of mitochondrial functions...
August 2016: FEMS Yeast Research
Ravindra Kumar Garg, Hardeep Singh Malhotra, Neeraj Kumar
Myeloneuropathy is a frequently encountered condition and often poses a diagnostic challenge. A variety of nutritional, toxic, metabolic, infective, inflammatory, and paraneoplastic disorders can present with myeloneuropathy. Deficiencies of vitamin B12, folic acid, copper, and vitamin E may lead to myeloneuropathy with a clinical picture of subacute combined degeneration of the spinal cord. Among infective causes, chikungunya virus has been shown to produce a syndrome similar to myeloneuropathy. Vacuolar myelopathy seen in human immunodeficiency virus (HIV) infection is clinically very similar to subacute combined degeneration...
April 2016: Annals of Indian Academy of Neurology
Min-Jing Li, Zhi-Ting Xiong, Hui Liu, Yi-Ming Kuo, Lei Tong
Hydroponic culture was used to comparatively investigate the copper (Cu)-induced alteration to sucrose metabolism and biomass allocation in two Elsholtzia haichowensis Sun populations with one from a Cu-contaminated site (CS) and the other from a non-contaminated site (NCS). Experimental results revealed that biomass allocation preferred roots over shoots in CS population, and shoots over roots in NCS population under Cu exposure. The difference in biomass allocation was correlated with the difference in sucrose partitioning between the two populations...
October 2, 2016: International Journal of Phytoremediation
Lavinia L Ruta, Claudia V Popa, Ioana Nicolau, Ileana C Farcasanu
To respond to metal surpluses, cells have developed intricate ways of defense against the excessive metallic ions. To understand the ways in which cells sense the presence of toxic concentration in the environment, the role of Ca(2+) in mediating the cell response to high Cu(2+) was investigated in Saccharomyces cerevisiae cells. It was found that the cell exposure to high Cu(2+) was accompanied by elevations in cytosolic Ca(2+) with patterns that were influenced not only by Cu(2+) concentration but also by the oxidative state of the cell...
December 2016: Environmental Science and Pollution Research International
Haiyan Zhang, Zhiyong Ni, Quanjia Chen, Zhongjun Guo, Wenwei Gao, Xiujuan Su, Yanying Qu
Drought, one of the most widespread factors reducing agricultural crop productivity, affects biological processes such as development, architecture, flowering and senescence. Although protein analysis techniques and genome sequencing have made facilitated the proteomic study of cotton, information on genetic differences associated with proteomic changes in response to drought between different cotton genotypes is lacking. To determine the effects of drought stress on cotton seedlings, we used two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry to comparatively analyze proteome of drought-responsive proteins during the seedling stage in two cotton (Gossypium hirsutum L...
June 2016: Molecular Genetics and Genomics: MGG
W D Klaren, G S Gadupudi, B Wels, D L Simmons, A K Olivier, L W Robertson
Polychlorinated Biphenyls (PCBs) are industrial chemicals that have become a persistent threat to human health due to ongoing exposure. A subset of PCBs, known as dioxin-like PCBs, pose a special threat given their potent hepatic effects. Micronutrients, especially Cu, Zn and Se, homeostatic dysfunction is commonly seen after exposure to dioxin-like PCBs. This study investigates whether micronutrient alteration is the byproduct of the ongoing hepatotoxicity, marked by lipid accumulation, or a concurrent, yet independent event of hepatic damage...
December 2, 2015: Toxicology
Chang Jia, Kai Zhang, Qilin Yu, Bing Zhang, Chenpeng Xiao, Yijie Dong, Yulu Chen, Biao Zhang, Laijun Xing, Mingchun Li
The vacuolar-type H+-ATPase (V-ATPase) is crucial for the maintenance of ion homeostasis. Dysregulation of ion homeostasis affects various aspects of cellular processes. However, the importance of V-ATPase in Candida albicans is not totally clear. In this study, we demonstrated the essential roles of V-ATPase through Tfp1, a putative V-ATPase subunit. Deletion of TFP1 led to generation of an iron starvation signal and reduced total iron content, which was associated with mislocalization of Fet34p that was finally due to disorders in copper homeostasis...
October 2015: Biochimica et Biophysica Acta
Zhongrui Xu, Chen Liu, Shenwen Cai, Luan Zhang, Zhiting Xiong
Vacuolar invertases (VINs) from Cu-tolerant and non-tolerant populations of Elsholtzia haichowensis have similar enzyme properties, and the enzyme protein divergences contribute little to the varied VIN activities between the contrasting populations. In our previous studies of Elsholtzia haichowensis, vacuolar invertase (VIN) activity in roots of a Cu-tolerant population was found to be significantly higher than that of a non-tolerant population under Cu stress. Divergences of amino acid residues in a sucrose-binding box and other regions of the VINs were detected...
October 2015: Plant Cell Reports
P S Paruruckumani, A Maharajan, V Ganapiriya, Y Narayanaswamy, R Raja Jeyasekar
Surface ultrastructure of the gill and liver of 3-month-old Asian sea bass, Lates calcarifer, after copper exposure, was investigated by scanning electron microscopy (SEM). Fish samples were exposed to copper concentrations of 6.83 and 13.66 ppm (sublethal) for 28 days with parallel untreated control. These structures showed structural modifications in both low and high concentrations of copper exposure. Oedema, hyperplasia, desquamation, necrosis, epithelial lifting, lamellar fusion, collapsed secondary lamellae, curling of secondary lamellae and aneurism in the secondary lamellae were observed in gill tissues exposed to copper...
December 2015: Biological Trace Element Research
Magdalena Migocka, Ewelina Posyniak, Ewa Maciaszczyk-Dziubinska, Anna Papierniak, Anna Kosieradzaka
Plant copper P1B-type ATPases appear to be crucial for maintaining copper homeostasis within plant cells, but until now they have been studied mostly in model plant systems. Here, we present the molecular and biochemical characterization of two cucumber copper ATPases, CsHMA5.1 and CsHMA5.2, indicating a different function for HMA5-like proteins in different plants. When expressed in yeast, CsHMA5.1 and CsHMA5.2 localize to the vacuolar membrane and are activated by monovalent copper or silver ions and cysteine, showing different affinities to Cu(+) (Km ∼1 or 0...
June 19, 2015: Journal of Biological Chemistry
Aleeza C Gerstein, Jasmine Ono, Dara S Lo, Marcus L Campbell, Anastasia Kuzmin, Sarah P Otto
Copper is a micronutrient essential for growth due to its role as a cofactor in enzymes involved in respiration, defense against oxidative damage, and iron uptake. Yet too much of a good thing can be lethal, and yeast cells typically do not have tolerance to copper levels much beyond the concentration in their ancestral environment. Here, we report a short-term evolutionary study of Saccharomyces cerevisiae exposed to levels of copper sulfate that are inhibitory to the initial strain. We isolated and identified adaptive mutations soon after they arose, reducing the number of neutral mutations, to determine the first genetic steps that yeast take when adapting to copper...
February 2015: Genetics
Catherine C Cortright, Sharon A Center, John F Randolph, Sean P McDonough, Kellie A Fecteau, Karen L Warner, Ann M Chiapella, Rhonda L Pierce, A Heather Graham, Linda J Wall, John H Heidgerd, Melisa A Degen, Patricia A Lucia, Hollis N Erb
OBJECTIVE: To characterize signalment, clinical features, clinicopathologic variables, hepatic ultrasonographic characteristics, endocrinologic profiles, treatment response, and age at death of Scottish Terriers with progressive vacuolar hepatopathy (VH) with or without hepatocellular carcinoma (HCC). DESIGN: Retrospective case series. ANIMALS: 114 Scottish Terriers with progressive VH. PROCEDURES: Electronic databases from 1980 to 2013 were searched for adult (age > 1 year) Scottish Terriers with histopathologic diagnoses of diffuse glycogen-like VH...
October 1, 2014: Journal of the American Veterinary Medical Association
Jianbin Wang, Tim Binks, Coral G Warr, Richard Burke
Copper and zinc homeostasis in polarized epithelial cells requires the correct localization and regulation of membrane-bound transport proteins at the apical and basolateral cell membranes. We have identified a subunit of the vacuolar-type H(+)-ATPase (V-ATPase) complex, vhaPPA1-2, and the Drosophila aquaporin homolog big brain (bib), as being required for the correct localization of the copper uptake transporters Ctr1A and Ctr1B and the zinc uptake protein dZip89B and hence necessary for optimal copper and zinc accumulation in vivo...
November 2014: Metallomics: Integrated Biometal Science
Liangtao Li, Ren Miao, Xuan Jia, Diane M Ward, Jerry Kaplan
Mmt1 and Mmt2 are highly homologous yeast members of the cation diffusion facilitator transporter family localized to mitochondria. Overexpression of MMT1/2 led to changes in cellular metal homeostasis (increased iron sensitivity, decreased cobalt sensitivity, increased sensitivity to copper), oxidant generation, and increased sensitivity to H2O2. The phenotypes due to overexpression of MMT1&2 were similar to that seen in cells with deletions in MRS3 and MRS4, genes that encode the mitochondrial iron importers...
June 13, 2014: Journal of Biological Chemistry
J M Bernard, K M Newkirk, A E McRee, J C Whittemore, E C Ramsay
Hepatic lesions in nondomestic felids are poorly characterized. The purpose of this study was to evaluate hepatic lesions in 90 captive, nondomestic felids including tigers, cougars, and lions. Hepatic lesions were histologically characterized as vacuolar change (lipidosis or glycogenosis), biliary cysts, biliary hyperplasia, hepatitis, necrosis, neoplasia, fibrosis, veno-occlusive disease, cholestasis, hematoma, congestion, or hemorrhage. Stepwise logistic regression analyses were performed for vacuolar change, benign biliary lesions, hepatitis, lipogranulomas, extramedullary hematopoiesis, and hepatic stellate cell hypertrophy and hyperplasia, with species as the outcome variable...
March 2015: Veterinary Pathology
Xuezhi Wang, Hui Wang, Jianxi Li, Zhiqiang Yang, Jingyan Zhang, Zhe Qin, Lei Wang, Xiaojun Kong
The present study was to evaluate the hepatotoxicity effects in mice exposed to copper (Cu) used as dietary supplements for 95 days. Cu-treated mice showed increased body weight, and no toxic symptoms were observed at the beginning, but the tendency gradually changed with progress of experiment. In the liver, beneficial metals [Cu, iron (Fe), zinc (Zn), manganese (Mn), and molybdenum (Mo)] were analyzed by flame atomic absorption spectrometry. The content of Cu maintained at the same level during the experiments, but not resulting in the imbalance of Fe, Zn, Mn, and Mo being distributed...
June 2014: Biological Trace Element Research
Ulrich Schlecht, Sundari Suresh, Weihong Xu, Ana Maria Aparicio, Angela Chu, Michael J Proctor, Ronald W Davis, Curt Scharfe, Robert P St Onge
BACKGROUND: Copper is essential for the survival of aerobic organisms. If copper is not properly regulated in the body however, it can be extremely cytotoxic and genetic mutations that compromise copper homeostasis result in severe clinical phenotypes. Understanding how cells maintain optimal copper levels is therefore highly relevant to human health. RESULTS: We found that addition of copper (Cu) to culture medium leads to increased respiratory growth of yeast, a phenotype which we then systematically and quantitatively measured in 5050 homozygous diploid deletion strains...
2014: BMC Genomics
Viviana Martins, Elias Bassil, Mohsen Hanana, Eduardo Blumwald, Hernâni Gerós
The Vitis vinifera copper transporter 1 is capable of self-interaction and mediates intracellular copper transport. An understanding of copper homeostasis in grapevine (Vitis vinifera L.) is particularly relevant to viticulture in which copper-based fungicides are intensively used. In the present study, the Vitis vinifera copper transporter 1 (VvCTr1), belonging to the Ctr family of copper transporters, was cloned and functionally characterized. Amino acid sequence analysis showed that VvCTr1 monomers are small peptides composed of 148 amino acids with 3 transmembrane domains and several amino acid residues typical of Ctr transporters...
July 2014: Planta
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