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Yuan-yuan Li, Zhao-qi Peng, Shi-lin He, Yan Ni, Xu-liang Hao
Thirteen compounds were isolated from the ethyl acetate fraction of Crepis crocea by column chromatographies on silica gel, Sephadex LH-20 and semi-preparative HPLC. The structures were elucidated on the basis of spectral analysis as tectorone I (1), 8β- (2-methyl- 2-hydroxy-3-oxobutanoyloxy) -glucozaluzanin C (2), tectoroside (3), luteolin-7-O-glucoside (4), cosmosiin (5), esculetin (6), 3,4-dihydroxybenzaldehyde (7), trans-4-hydroxycinnamic acid (8), Caffeic acid (9), methyl p-hydroxyphenyllactate (10), ethylp- hydroxyphenyllactate (11), cis-3,4-dihydroxy-β-ionion (12)...
October 2015: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
N V Beloborodova, V V Moroz, A A Osipov, A V Vlasenko, K M Fateev, Yu N Sarshor, A K Pautova, A Yu Bedova
INTRODUCTION: Simple prognostic marker search remains relevant for patients with acute abdomen. Previously it was shown that the levels of aromatic amino acid metabolites (phenyl carboxylic acids - PhCAs) are significantly increased in septic and other critically ill patients []. METHODS: In the present study we performed ROC-analysis for total level of four clinically significant PhCAs, namely: phenylacetic(PAA), phenyllactic(PLA), p-hydroxyphenyllactic(p-HPLA), p- hydroxyphenylacetic(p-HPAA): ∑4PhCAs...
October 2015: Shock
M Trent Herdman, Natthida Sriboonvorakul, Stije J Leopold, Sam Douthwaite, Sanjib Mohanty, M Mahtab Uddin Hassan, Richard J Maude, Hugh W F Kingston, Katherine Plewes, Prakaykaew Charunwatthana, Kamolrat Silamut, Charles J Woodrow, Aniruddha Ghose, Kesinee Chotinavich, Md Amir Hossain, M Abul Faiz, Saroj Mishra, Natchanun Leepipatpiboon, Nicholas J White, Nicholas P J Day, Joel Tarning, Arjen M Dondorp
INTRODUCTION: Severe falciparum malaria is commonly complicated by metabolic acidosis. Together with lactic acid (LA), other previously unmeasured acids have been implicated in the pathogenesis of falciparum malaria. METHODS: In this prospective study, we characterised organic acids in adults with severe falciparum malaria in India and Bangladesh. Liquid chromatography-mass spectrometry was used to measure organic acids in plasma and urine. Patients were followed until recovery or death...
2015: Critical Care: the Official Journal of the Critical Care Forum
N V Beloborodova, V V Moroz, A A Osipov, A Yu Bedova, A Yu Olenin, M L Getsina, O V Karpova, E G Olenina
Previous studies showed that large amounts of phenylcarboxylic acids (PhCAs) are accumulated in a septic patient's blood due to increased endogenous and microbial phenylalanine and tyrosine biotransformation. Frequently, biochemical aromatic amino acid transformation into PhCAs is considered functionally insignificant for people without monogenetic hereditary diseases. The blood of healthy people contains the same PhCAs that are typical for septic patients as shown in this paper. The overall serum PhCAs level was 6 µM on average as measured by gas chromatography with flame ionization detection...
March 2015: Biochemistry. Biokhimii︠a︡
Claudia Axel, Bettina Röcker, Brid Brosnan, Emanuele Zannini, Ambrose Furey, Aidan Coffey, Elke K Arendt
The present study investigated the antifungal activity of Lactobacillus amylovorus DSM19280 as a starter culture for gluten-free quinoa sourdough bread under pilot-plant conditions to extend the microbial shelf life. Challenge tests against environmental moulds were conducted and a negative control with non-antifungal strain, L. amylovorus DSM20531(T), as well as a chemically acidified and a non-acidified control were included. Organic acid production, antifungal metabolites, carbohydrates changes during fermentation and bread quality were compared to wheat counterparts...
May 2015: Food Microbiology
Hyun-Jung Choi, Hae In Bang, Chang-Seok Ki, Soo-Youn Lee, Jong-Won Kim, Junghan Song, Mee-Ran Shin, Yong-Wha Lee, Dong Hwan Lee, Hyung-Doo Park
BACKGROUND: Hereditary tyrosinemia type I (HT I) is a severe inborn metabolic disorder affecting the tyrosine degradation pathway. Most untreated patients die within the first two years of life. HT I results from fumarylacetoacetate hydrolase (FAH) deficiency caused by mutations in the FAH gene. The diagnosis of HT I is confirmed by measuring FAH enzyme activity in cultured fibroblasts or liver tissue and/or detecting disease-causing mutations in the FAH gene. METHODS: A female neonate was referred to our hospital for further evaluation of an abnormal newborn screening test that showed elevated tyrosine levels...
2014: Annals of Clinical and Laboratory Science
N V Beloborodova, A A Osipov, A Iu Bedova
Physico-chemical and biological properties of sepsis-associated low molecular aromatic compounds are summarized and analysed in the review. Phenylcarbonic acids (PCAs), such as para-hydroxyphenyllactic acid (p-HPLA), phenyllactic acid (PLA), para-hydroxyphenylacetic (p-HPAA), phenylacetic acid (PAA), benzoic acid (BA), and phenylpropionic acid (PPA) are shown to have biorequlatory activity and be able to affect both bacteria and eukaryotic cells. In the review there are presented data on the diagnostic and pathogenetic value of PCAs, their bacteriostatic and bacteriocidal properties and biosynthesis by clinically significant bacterial species, as well as description of the mechanisms of microbial resistance to PCAs, the pathways of PCAs metabolism by prokaryotes, PCAs membrane transport and excretion pathways in humans, the data on the use of PCAs in clinical practice...
2013: Antibiotiki i Khimioterapii︠a︡, Antibiotics and Chemoterapy [sic]
Natthida Sriboonvorakul, Natchanun Leepipatpiboon, Arjen M Dondorp, Thomas Pouplin, Nicholas J White, Joel Tarning, Niklas Lindegardh
Acidosis is an important cause of mortality in severe falciparum malaria. Lactic acid is a major contributor to metabolic acidosis, but accounts for only one-quarter of the strong anion gap. Other unidentified organic acids have an independent strong prognostic significance for a fatal outcome. In this study, a simultaneous bio-analytical method for qualitative and quantitative assessment in plasma and urine of eight small organic acids potentially contributing to acidosis in severe malaria was developed and validated...
December 15, 2013: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Shira Elkobi-Peer, Raya Faigenbaum, Shmuel Carmeli
Five new natural products, aeruginosins GE686 (1), GE766 (2), GE730 (3), GE810 (4), and GE642 (5), were isolated along with four known aeruginosins, 98C, 101, KY642, and DA688, from bloom material of the cyanobacterium Microcystis aeruginosa collected from a fish pond in Kibbutz Geva, Israel, in August 2007. Their structures were elucidated by a combination of various spectroscopic techniques, primarily NMR and MS, while the absolute configurations of the stereogenic centers were determined by Marfey's and chiral-phase HPLC methods...
December 28, 2012: Journal of Natural Products
Natalia Beloborodova, Iskander Bairamov, Andrei Olenin, Victoria Shubina, Vera Teplova, Nadezhda Fedotcheva
BACKGROUND: Several low-molecular-weight phenolic acids are present in the blood of septic patients at high levels. The microbial origin of the most of phenolic acids in the human body was shown previously, but pathophysiological role of the phenolic acids is not clear. Sepsis is associated with the excessive production of reactive oxygen species (ROS) in both the circulation and the affected organs. In this work the influence of phenolic acids on ROS production in mitochondria and neutrophils was investigated...
2012: Journal of Biomedical Science
Faruk Lumanul Hakkim, Mathiraj, Musthafa Mohamed Essa, Girija Arivazhagan, Nejib Guizani, Song Hyuk
The present study evaluated the antioxidant (AA), antimicrobial and preservation effects of five plant derived natural products viz., Rosmarinic Acid (RA), p-Coumaric Acid (pCA), Trans-Cinnamic Acid (TCA), Hydroxyphenyllactic Acid (HPA) and Caffeic acid (CA) along with synthetic compounds (Ascorbic acid, gallic acid, citric acid and BHA) on fresh cut apple slices. Antimicrobial efficacy of these compounds against Bacillus licheniformis, Pseudomonas vulgaris, Shigella boydii, Salmonella typhi, Staphylococcus aureus, Listeria monocytogenes and Escherichia coli was found to be concentration dependent with the maximum inhibition observed at 500 microg mL(-1)...
January 1, 2012: Pakistan Journal of Biological Sciences: PJBS
A M Dallagnol, C A N Catalán, M I Mercado, G Font de Valdez, G C Rollán
AIM: To evaluate the influence of biosynthetic precursors, intermediates and electron acceptors on the production of antifungal compounds [phenyllactic acid (PLA) and hydroxyphenyllactic acid (OH-PLA)] by Lactobacillus plantarum CRL 778, a strain isolated from home-made sourdough. METHODS AND RESULTS: Growth of fermentative activity and antifungal compounds production by Lact. plantarum CRL 778 were evaluated in a chemically defined medium (CDM) supplemented with biosynthetic precursors [phenylalanine (Phe), tyrosine (Tyr)], intermediates [glutamate (Glu), alpha-ketoglutarate (α-KG)] and electron acceptors [citrate (Cit)]...
December 2011: Journal of Applied Microbiology
B Markaverich, T Schauweker, R Gregory, R Varma, M Varma, F Kittrell, D Medina
Methyl p-hydroxyphenyllactate (MeHPLA) is an endogenous ligand for nuclear type II sites which apparently regulates cellular growth and proliferation through this binding interaction. Occupancy of type II sites by MeHPLA agonists such as dihydroxybenzylidene acetophenone (DHBA), 2,6-bis ([3,4-dihydroxyphenyl]-methylene) cyclohexanone (BDHPC) and 2,6-bis ([3-methoxy-4-hydroxyphenyl]-methylene)cyclohexanone (BMHPC) is directly correlated with the inhibition of malignant cell proliferation. Most importantly, these compounds inhibit mammary tumor growth in vivo with minimal non-specific cytotoxicity...
March 1993: International Journal of Oncology
B Markaverich, M Varma, C Densmore, A Tiller, T Schauweker, R Gregory
Previous studies from this laboratory demonstrated that 2,6-bis-([3,4-dihydroxyphenyl]methylene)cyclohexanone (BDHPC) and related compounds mimic methyl p-hydroxyphenyllactate (MeHPLA) as endogenous ligands for nuclear type II [H-3]estradiol binding sites. Occupancy of type II sites by these agents results in the inhibition of malignant cell proliferation in vitro and mammary tumor growth in vivo. The present studies were designed to assess the effects of BDHPC esterification on type II site binding interactions in uterine nuclei and in cultured MCF-7 human breast cancer cells in vitro...
June 1994: International Journal of Oncology
N V Beloborodova, I T Baĭramov, A Iu Olenin, N I Fedotcheva
Some exometabolites produced by basic representatives of human anaerobic microflora were investigated, detected by gas chromatography-mass spectrometry (GC-MS). In vitro besides lactic acid Bifidobacterium and Lactobacillus generate substantial amounts of phenyllactic and p-hydroxyphenyllactic acids. Clostridium produced 2-hydroxybutyric acid and to a lesser extent lactic and phenyllactic acids. In contrast to C. perfringens, C. sporogenes generates substantial amount of phenylpropionic and p-hydroxyphenylpropionic acids and less p-hydroxyphenyllactic acid...
January 2011: Biomedit︠s︡inskai︠a︡ Khimii︠a︡
Tomiko Kuhara, Morimasa Ohse, Yoshito Inoue, Arthur J L Cooper
Citrin is the hepatic mitochondrial aspartate-glutamate carrier that is encoded by the gene SLC25A13. Citrin deficiency often leads to hyperammonemia, for which the current treatment concept is different from that for primary hyperammonemias. Metabolite level diagnosis, often referred to as chemical diagnosis, is not always successful in identifying citrin deficiency immediately or in a timely fashion. We previously made the chemical diagnosis of citrin deficiency in ten patients from nine families. In order to devise a more rapid and more accurate chemical diagnosis of this disorder than is currently available, we reinvestigated the gas chromatography/mass spectrometry-based urine metabolome in these patients...
June 2011: Analytical and Bioanalytical Chemistry
Verena Janiak, Maike Petersen, Matthias Zentgraf, Gerhard Klebe, Andreas Heine
Hydroxy(phenyl)pyruvate reductase [H(P)PR] belongs to the family of D-isomer-specific 2-hydroxyacid dehydrogenases and catalyzes the reduction of hydroxyphenylpyruvates as well as hydroxypyruvate and pyruvate to the corresponding lactates. Other non-aromatic substrates are also accepted. NADPH is the preferred cosubstrate. The crystal structure of the enzyme from Coleus blumei (Lamiaceae) has been determined at 1.47 A resolution. In addition to the apoenzyme, the structure of a complex with NADP(+) was determined at a resolution of 2...
May 2010: Acta Crystallographica. Section D, Biological Crystallography
N V Beloborodova, A S Khodakova, I T Bairamov, A Yu Olenin
In previous studies we demonstrated increased amounts of phenylcarboxylic acids (PCA) in serum of patients with sepsis. This observation prompted the present study of the ability of the human microbiome bacteria to produce PCA in vitro. PCA were detected in culture media by gas chromatography-mass spectrometry. Increased amounts of phenyllactic and p-hydroxyphenyllactic acids were produced by Klebsiella pneumonia, Escherichia coli, and Staphylococcus aureus. Certain strict anaerobes (bifidobacteria, lactobacteria, eubacteria) have also been found to actively produce these PCA, but these bacteria are not etiologically linked to sepsis...
December 2009: Biochemistry. Biokhimii︠a︡
Maike Petersen, Yana Abdullah, Johannes Benner, David Eberle, Katja Gehlen, Stephanie Hücherig, Verena Janiak, Kyung Hee Kim, Marion Sander, Corinna Weitzel, Stefan Wolters
Rosmarinic acid and chlorogenic acid are caffeic acid esters widely found in the plant kingdom and presumably accumulated as defense compounds. In a survey, more than 240 plant species have been screened for the presence of rosmarinic and chlorogenic acids. Several rosmarinic acid-containing species have been detected. The rosmarinic acid accumulation in species of the Marantaceae has not been known before. Rosmarinic acid is found in hornworts, in the fern family Blechnaceae and in species of several orders of mono- and dicotyledonous angiosperms...
October 2009: Phytochemistry
N I Fedotcheva, R E Kazakov, M N Kondrashova, N V Beloborodova
Low-molecular-weight phenolic acids (PhAs) phenylacetate, phenyllactate, phenylpropionate, p-hydroxyphenyllactate, and p-hydroxyphenylacetate are essentially the products of the degradation of aromatic amino acids and polyphenols by the intestinal microflora. In sepsis, the concentrations of some of these acids in the blood increase tens of times. Assuming that these compounds can cause the mitochondrial dysfunction in sepsis, we examined their effects on respiration, the induction of pore opening, and the production of reactive oxygen species (ROS) in mitochondria...
August 28, 2008: Toxicology Letters
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