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Bernd Markus Lange, Justin T Fischedick, Malte F Lange, Narayanan Srividya, Dunja Samec, Brenton C Poirier
Members of the genus Tripterygium are known to contain an astonishing diversity of specialized metabolites. The lack of authentic standards has been an impediment to the rapid identification of such metabolites in extracts. We employed an approach that involves the searching of multiple, complementary chromatographic and spectroscopic data sets against the Spektraris database to speed up the metabolite identification process. Mass spectrometry-based imaging indicated a differential localization of triterpenoids to the periderm and sesquiterpene alkaloids to the cortex layer of Tripterygium roots...
November 18, 2016: Plant Physiology
S Akroum
Human and animal mycoses become more frequent and more resistant to traditional treatments. In this work, we tested the in vitro antifungal activity of acetonic extracts of Punica granatum L., Quercus suber L. and Vicia faba L. against seven pathogen fungi and the in vivo antifungal activity against Candida albicans and Trichophyton mentagrophytes. The phytochemical screening was also carried out and showed that the extracts contained mainly proanthocyanidins. Other polyphenols were also present but in low quantity...
November 14, 2016: Journal de Mycologie Médicale
Carole Beaulieu, Amadou Sidibé, Raoudha Jabloune, Anne-Marie Simao-Beaunoir, Sylvain Lerat, Ernest Monga, Mark A Bernards
Potato peels consist of a tissue called phellem, which is formed by suberized cell layers. The degradation of suberin, a lipidic and recalcitrant polymer, is an ecological process attributed to soil fungal populations; however, previous studies have suggested that Streptomyces scabiei, the causal agent of potato common scab, possesses the ability to degrade suberin. In the present study, S. scabiei was grown in medium containing suberin-enriched potato phellem as the sole carbon source and its secretome was analyzed periodically (10- to 60-d-old cultures) with a special focus on proteins potentially involved in cell wall degradation...
November 17, 2016: Microbes and Environments
Ana Lourenço, Jorge Rencoret, Catarina Chemetova, Jorge Gominho, Ana Gutiérrez, José C Del Río, Helena Pereira
The composition and structure of lignin in different tissues-phellem (cork), phloem and xylem (wood)-of Quercus suber was studied. Whole cell walls and their respective isolated milled lignins were analyzed by pyrolysis coupled with gas chromatography/mass spectrometry (Py-GC/MS), two-dimensional nuclear magnetic resonance spectroscopy (2D-NMR) and derivatization followed by reductive cleavage (DFRC). Different tissues presented varied p-hydroxyphenyl:guaiacyl:syringyl (H:G:S) lignin compositions. Whereas lignin from cork has a G-rich lignin (H:G:S molar ratio 2:85:13), lignin from phloem presents more S-units (H:G:S molar ratio of 1:58:41) and lignin from xylem is slightly enriched in S-lignin (H:G:S molar ratio 1:45:55)...
2016: Frontiers in Plant Science
Xiaoqiang Liu, Pepipei Liu, Xiaohe Huo, Xiuhua Liu, Jin Liu
TiO2 nanosheets (TNSs) were synthesized and deposited on multi-wall carbon nanotubes (MWCNTs) to form a nano-composite through a hydrothermal method, followed by the characterization with various spectroscopic and microscopic techniques. The TNS-MWCNT composite was then applied as not only an electrode scaffold to immobilize primary antibody, but also as a carrier to load secondary antibody and horseradish peroxidase (HRP). In both cases, bis(sulfosuccinimidyl) suberate sodium salt acted as an amino cross-linker to covalently bind the biomolecules on TNS-MWCNT composite through their surface primary amino groups...
November 23, 2016: Analytica Chimica Acta
A Abdessamad, G Baraket, H Sakka, Y Ammari, M Ksontini, A Salhi Hannachi
Sequences of the trnL-trnF spacer and combined trnL-trnF region in chloroplast DNA of cork oak (Quercus suber L.) were analyzed to detect polymorphisms and to elucidate molecular evolution and demographic history. The aligned sequences varied in length and nucleotide composition. The overall ratio of transition/transversion (ti/tv) of 0.724 for the intergenic spacer and 0.258 for the pooled sequences were estimated, and indicated that transversions are more frequent than transitions. The molecular evolution and demographic history of Q...
October 24, 2016: Genetics and Molecular Research: GMR
Marica T Engström, Xiaowei Sun, Matthew P Suber, Min Li, Juha-Pekka Salminen, Ann E Hagerman
Many food and forage plants contain tannins, high molecular weight polyphenols that characteristically interact strongly with protein, forming complexes that affect taste, nutritional quality and health of the consumer. In the present study, the interaction between bovine serum albumin (BSA), and each of seven hydrolyzable tannins or epigallocatechin gallate was examined. The objective was to define the effect of tannin oxidation, measured as oxidative activity (browning) or as oxidizability (degradation monitored by HPLC) on the formation on highly stabilized tannin-protein complexes, and to determine how the reaction depended on the pH conditions...
November 3, 2016: Journal of Agricultural and Food Chemistry
Presentini Rivo
A new method has been developed to prepare horseradish peroxidase (HRP) conjugates using bis(sulfosuccinimidyl)suberate (BS3) as cross-linking reagent in a two-step procedure. The enzyme is reacted with BS3, introducing active ester molecules into the enzyme itself, and it is then used directly to label amino-containing compounds without further treatment. The proteins involved in the conjugation undergo minimal modifications. The reaction conditions are evaluated, as well as studies on the conservation of the biological activities of the conjugated proteins and the stability of the conjugates in time...
October 31, 2016: Journal of Immunoassay & Immunochemistry
Michael G Ficklin, Michael P Kowaleski, Kevin A R Kunkel, Jonathan T Suber
A two-year-old, 44 kg dog with a right Helica cementless total hip replacement (THR) was radiographically diagnosed with implant loosening eight months after the index total hip replacement procedure. Subsequent synoviocentesis and synovial fluid culture revealed a methicillin-resistant coagulase-negative Staphylococcus spp infection of the right THR. A one-stage revision using a hybrid BFX cementless acetabular cup and CFX cemented femoral stem was performed. Vancomycin and micro-silver antimicrobial powder impregnated cement were used in the revision...
October 20, 2016: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
Sylvain Legay, Gea Guerriero, Christelle André, Cédric Guignard, Emmanuelle Cocco, Sophie Charton, Marc Boutry, Owen Rowland, Jean-Francois Hausman
A comparison of the transcriptomes of russeted vs nonrusseted apple skins previously highlighted a tight relationship between a gene encoding an MYB-type transcription factor, MdMYB93, and some key suberin biosynthetic genes. The present work assesses the role of this transcription factor in the suberization process. A phylogenetic analysis of MdMYB93 and Arabidopsis thaliana MYBs was performed and the function of MdMYB93 was further investigated using Agrobacterium-mediated transient overexpression in Nicotiana benthamiana leaves...
September 15, 2016: New Phytologist
Rosalia Deeken, Stefanie Saupe, Jörn Klinkenberg, Michael Riedel, Jana Leide, Rainer Hedrich, Thomas Mueller
Non-specific lipid transfer proteins (nsLTPs) reversibly bind different types of lipid molecules in a hydrophobic cavity. They facilitate phospholipid transfer between membranes in vitro, play a role in cuticle and possibly in suberin formation, and might be involved in plant pathogen defense signaling. This study focuses on the role of AtLTPI-4 in crown gall development. Arabidopsis thaliana crown gall tumors, which develop upon infection with the virulent Agrobacterium tumefaciens strain C58, highly expressed the type I nsLTP gene AtLTPI-4...
September 29, 2016: Plant Physiology
Andreia F Sousa, Alessandro Gandini, Ana Caetano, Teresa M R Maria, Carmen S R Freire, Carlos Pascoal Neto, Armando J D Silvestre
The main purpose of this study was to investigate the potential of suberin (a naturally occurring aromatic-aliphatic polyester ubiquitous to the vegetable realm) as a renewable source of chemicals and, in particular, to assess their physical properties. A comparison between cork and birch suberin fragments obtained by conventional depolymerisation processes (hydrolysis or methanolysis) is provided, focusing essentially on their thermal and crystallinity properties. It was found that suberin fragments obtained by the hydrolysis depolymerisation of birch had a high degree of crystallinity, as indicated by their thermal analysis and corroborated by the corresponding XRD diffractions, as opposed to hydrolysis-depolymerised cork suberin counterparts, which were essentially amorphous...
September 12, 2016: International Journal of Biological Macromolecules
Suber S Huang, Chad Marcantonio
No abstract text is available yet for this article.
September 2016: Asia-Pacific Journal of Ophthalmology
Justin Graham Lashbrooke, Hagai Cohen, Dorit Levy-Samocha, Oren Tzfadia, Irina Panizel, Viktoria Zeisler, Hassan Massalha, Adi Stern, Livio Trainotti, Lukas Schreiber, Fabrizio Costa, Asaph Aharoni
Suberin, a polymer composed of both aliphatic and aromatic domains, is deposited as a rough matrix upon plant surface damage and during normal growth in the root endodermis, bark, specialized organs (e.g., Solanum tuberosum (potato) tubers), and seed coats. To identify genes associated with the developmental control of suberin deposition, we investigated the chemical composition and transcriptomes of suberized Solanum lycopersicum (tomato) and russet apple (Malus x domestica) fruit surfaces. Consequently, a gene expression signature for suberin polymer assembly was revealed that is highly conserved in angiosperms...
September 7, 2016: Plant Cell
Madori Osawa, Sohtaro Mine, Shinichiro Ota, Kengo Kato, Tsuyoshi Sekizuka, Makoto Kuroda, Michiyo Kataoka, Hitomi Fukumoto, Yuko Sato, Takayuki Kanno, Hideki Hasegawa, Keiji Ueda, Masashi Fukayama, Takuya Maeda, Soichiro Kanoh, Akihiko Kawana, Yuji Fujikura, Harutaka Katano
BACKGROUND: Primary effusion lymphoma is a rare distinct large B-cell neoplasm that is associated with Kaposi's sarcoma-associated herpesvirus (KSHV) infection. Over recent years, 9 KSHV-positive/Epstein-Barr virus (EBV)-negative PEL cell lines have been established. METHODS: Tumor cells were collected from the pleural effusion of a 49-year-old male with AIDS. Cells were grown in RPMI1640 culture medium supplemented with 10 % fetus bovine serum. Single cell cloning was performed successfully by a limiting dilution method in a 96-well plate...
2016: Infectious Agents and Cancer
Roger Verdaguer, Marçal Soler, Olga Serra, Aïda Garrote, Sandra Fernández, Dolors Company-Arumí, Enriqueta Anticó, Marisa Molinas, Mercè Figueras
Suberin and wax deposited in the cork (phellem) layer of the periderm form the lipophilic barrier that protects mature plant organs. Periderm lipids have been widely studied for their protective function with regards to dehydration and for how they respond to environmental stresses and wounding. However, despite advances in the biosynthetic pathways of suberin and associated wax, little is known about the regulation of their deposition. Here, we report on a potato NAC transcription factor gene, StNAC103, induced in the tuber phellem (skin)...
October 2016: Journal of Experimental Botany
Suber S Huang, Katherine High, Raffaella Toso
No abstract text is available yet for this article.
July 2016: Asia-Pacific Journal of Ophthalmology
Devrim Coskun, Dev T Britto, Wayne Q Huynh, Herbert J Kronzucker
Although deemed a "non-essential" mineral nutrient, silicon (Si) is clearly beneficial to plant growth and development, particularly under stress conditions, including salinity and drought. Here, we review recent research on the physiological, biochemical, and molecular mechanisms underlying Si-induced alleviation of osmotic and ionic stresses associated with salinity and drought. We distinguish between changes observed in the apoplast (i.e., suberization, lignification, and silicification of the extracellular matrix; transpirational bypass flow of solutes and water), and those of the symplast (i...
2016: Frontiers in Plant Science
Devon H Ghodasra, Adrienne Chen, J Fernando Arevalo, David G Birch, Kari Branham, Brian Coley, Gislin Dagnelie, Eugene de Juan, Robert G Devenyi, Jessy D Dorn, Andy Fisher, Duane R Geruschat, Ninel Z Gregori, Robert J Greenberg, Paul Hahn, Allen C Ho, Ashley Howson, Suber S Huang, Raymond Iezzi, Naheed Khan, Byron L Lam, Jennifer I Lim, Kirsten G Locke, Michelle Markowitz, Anne-Marie Ripley, Mark Rankin, Hannah Schimitzek, Fay Tripp, James D Weiland, Jiong Yan, David N Zacks, K Thiran Jayasundera
No abstract text is available yet for this article.
2016: BMC Ophthalmology
Michael G Ficklin, Kevin A R Kunkel, Jonathan T Suber, Patrick D Gerard, Michael P Kowaleski
OBJECTIVES: Numerous studies have examined the biomechanics of polymethyl methacrylate (PMMA) with added antibiotics, but direct comparison between studies is difficult. Our purpose was to evaluate the effects of the addition of antibiotic drugs and silver on compressive and bending strength of PMMA. Our null hypothesis was that there would be no significant difference in the compressive strength or bending strength of PMMA with the addition of silver or varying amounts of antibiotic drugs...
September 20, 2016: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
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