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https://www.readbyqxmd.com/read/28506468/-impact-of-cork-oak-management-on-the-ectomycorrhizal-fungal-diversity-associated-with-quercus-suber-in-the-m%C3%A3-amora-forest-morocco
#1
Fatima Z Maghnia, Hervé Sanguin, Younes Abbas, Marcello Verdinelli, Benaissa Kerdouh, Naima El Ghachtouli, Enrico Lancellotti, Salah Eddine Bakkali Yakhlef, Robin Duponnois
The cork oak forest is an ecosystem playing a major role in Moroccan socio-economy and biodiversity conservation. However, this ecosystem is negatively impacted by extensive human- and climate-driven pressures, causing a strong decrease in its distribution and a worsening of the desertification processes. This study aims at characterising the impact of cork oak forest management on a major actor of its functioning, the ectomycorrhizal (EcM) fungal community associated with Quercus suber, and the determination of EcM bio-indicators...
May 11, 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28443925/the-effect-of-ligand-ligand-interactions-on-the-formation-of-photoluminescent-gold-nanoclusters-embedded-in-au-i-thiolate-supramolecules
#2
Hsiang-Yu Chang, Yu-Ting Tseng, Zhiqin Yuan, Hung-Lung Chou, Ching-Hsiang Chen, Bing-Joe Hwang, Meng-Che Tsai, Huan-Tsung Chang, Chih-Ching Huang
In this study, we prepared photoluminescent l-cysteine (Cys)-capped gold nanoclusters (Cys-Au NCs) via NaBH4-mediated reduction of aggregated coordination polymers (supramolecules) of -[Cys-Au(i)]n-. The -[Cys-Au(i)]n- supramolecules with interesting chiral properties were formed through simple reactions of chloroauric acid (HAuCl4) with Cys at certain pH values (pH 3-7). The -[Cys-Au(i)]n- polymers could self-assemble into -[Cys-Au(i)]n- supramolecules with irregular morphologies and diameters larger than 500 nm through stacked hydrogen bonding and zwitterionic interactions between Cys ligands and through Au(i)Au(i) aurophilic interactions in solutions with pH values ≤7...
May 17, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28419920/the-retention-and-distribution-of-parent-alkylated-and-n-o-s-containing-polycyclic-aromatic-hydrocarbons-on-the-epidermal-tissue-of-mangrove-seedlings
#3
Ruilong Li, Huadong Tan, Yaxian Zhu, Yong Zhang
The polycyclic aromatic hydrocarbons (PAHs) located on the epidermal tissues showed distinctive toxic effects to root, while the retention and distribution of PAHs on mangrove seedlings poorly understood. Our results confirmed that the partition coefficients (Kf) of the PAHs retained on the epidermal tissue of mangrove roots, such as Kandelia obovata, Avicennia marina and Aegiceras corniculatum, were much higher than the Poaceae plants roots, for example wheat and maize (Wild et al., 2005). Moreover, to the parent and alkyl PAHs, a well negative correlation was observed between the surface polarity of these three species of mangrove root and the Kf values (p < 0...
April 15, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28419433/plant-soil-feedbacks-in-declining-forests-implications-for-species-coexistence
#4
Lorena Gómez-Aparicio, Jara Domínguez-Begines, Paul Kardol, José M Ávila, Beatriz Ibáñez, Luis V García
Plant-soil feedbacks (PSFs) play a relevant role as drivers of species abundance, coexistence, and succession in plant communities. However, the potential contribution of PSFs to community dynamics in changing forest ecosystems affected by global change drivers is still largely unexplored. We measured the direction, strength and nature (biological vs. chemical) of PSFs experienced by coexisting tree species in two types of declining Quercus suber forests of southwestern Spain (open woodland vs. closed forest) invaded by the exotic soil pathogen Phytophthora cinnamomi...
April 18, 2017: Ecology
https://www.readbyqxmd.com/read/28402676/optimizing-the-parameters-governing-the-fragmentation-of-cross-linked-peptides-in-a-tribrid-mass-spectrometer
#5
Lars Kolbowski, Marta L Mendes, Juri Rappsilber
We compared the five different ways of fragmentation available on a tribrid mass spectrometer and optimized their collision energies with regard to optimal sequence coverage of cross-linked peptides. We created a library of bis(sulfosuccinimidyl)suberate (BS3/DSS) cross-linked precursors, derived from the tryptic digests of three model proteins (Human Serum Albumin, creatine kinase, and myoglobin). This enabled in-depth targeted analysis of the fragmentation behavior of 1065 cross-linked precursors using the five fragmentation techniques: collision-induced dissociation (CID), beam-type CID (HCD), electron-transfer dissociation (ETD), and the combinations ETciD and EThcD...
May 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28351588/synthesis-and-anti-hiv-activities-of-unsymmetrical-long-chain-dicarboxylate-esters-of-dinucleoside-reverse-transcriptase-inhibitors
#6
Hitesh K Agarwal, Bhupender S Chhikara, Gustavo F Doncel, Keykavous Parang
A series of 11 unsymmetrical dicarboxylate conjugates of dinucleoside reverse transcriptase inhibitors were synthesized. Three dicarboxylic acids, succinic acid, suberic acid and 1,14-tetradecandioc acid, were diesterified with either 3'-azido-2',3'-dideoxythymidine (AZT), 3'-fluoro-2',3'-dideoxythymidine (FLT), 2',3'-dideoxy-3'-thiacytidine (3TC), or 5-fluoro-2',3'-dideoxy-3'-thiacytidine (FTC). The anti-HIV activity of synthesized compounds was evaluated against HIV-1 X4 (IIIB) and R5 (BaL) viral strains in single-round infection assays...
May 1, 2017: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/28348461/am966-an-antagonist-of-lysophosphatidic-acid-receptor-1-increases-lung-microvascular-endothelial-permeability-through-activation-of-rho-signaling-pathway-and-phosphorylation-of-ve-cadherin
#7
Junting Cai, Jianxin Wei, Shuang Li, Tomeka Suber, Jing Zhao
Maintenance of pulmonary endothelial barrier integrity is important for reducing severity of lung injury. Lysophosphatidic acid (LPA) regulates cell motility, cytoskeletal rearrangement, and cell growth. Knockdown of LPA receptor 1 (LPA1) has been shown to mitigate lung injury and pulmonary fibrosis. AM966, an LPA1 antagonist exhibiting an antifibrotic property, has been considered to be a future antifibrotic medicine. Here, we report an unexpected effect of AM966, which increases lung endothelial barrier permeability...
2017: Mediators of Inflammation
https://www.readbyqxmd.com/read/28323849/bis-sulfosuccinimidyl-suberate-bs3-crosslinking-analysis-of-the-behavior-of-amyloid-%C3%AE-peptide-in-solution-and-in-phospholipid-membranes
#8
Jing-Ming Shi, Jie Pei, En-Qi Liu, Lin Zhang
The structure and state of amyloid-β peptide (Aβ) oligomers often need to be checked by reliable experimental methods. Electrophoresis is a commonly applied measurement method. However, due to the presence of detergents, oligomers are easily broken during electrophoresis, which makes it very hard to accurately assess Aβ aggregate states. In the current study, bis(sulfosuccinimidyl) suberate (BS3) was used to cross-link Aβ1-42 oligomers prior to electrophoresis. When compared to a previously reported Aβ cross-linking agent, glutaraldehyde, it was quite apparent that BS3 is more suitable for detecting intra-membrane Aβ oligomers and extra-membrane Aβ oligomers states...
2017: PloS One
https://www.readbyqxmd.com/read/28298444/scf-fbxo17-e3-ligase-modulates-inflammation-by-regulating-proteasomal-degradation-of-glycogen-synthase-kinase-3%C3%AE-in-lung-epithelia
#9
Tomeka Suber, Jianxin Wei, Anastasia M Jacko, Ina Nikolli, Yutong Zhao, Jing Zhao, Rama K Mallampalli
Glycogen synthase kinase-3β (GSK3β) has diverse biological roles including effects on cellular differentiation, migration, and inflammation. GSK3β phosphorylates proteins to generate phosphodegrons necessary for recognition by Skp1/Cullin-1/F-box (SCF) E3 ubiquitin ligases leading to subsequent proteasomal degradation of these substrates. However, little is known regarding how GSK3β protein stability itself is regulated and how its stability may influence inflammation. Here we show that GSK3β is degraded by the ubiquitin-proteasome pathway in murine lung epithelial cells through lysine 183 as an acceptor site for K48 polyubiquitination...
May 5, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28268127/spectrophotometric-method-for-the-quantitative-assay-of-n-hydroxysulfosuccinimide-esters-including-extinction-coefficients-and-reaction-kinetics
#10
Rivo Presentini
A quantitative spectrophotometric method has been developed for the analysis of N-hydroxysulfosuccinimide (sulfo-NHS), a chromophore with a maximum absorbance at 268 nm. The extinction coefficients were determined between pH 6.0 and 8.0 and found to vary in a nonlinear manner. This spectrophotometric profile is not present in its esters which however release an equimolar amount of sulfo-NHS when they react with nucleophilic groups or hydrolyze in aqueous solution. This fact facilitates the determination in solution of the concentration and purity of bis(sulfosuccinimidyl) suberate (BS3) used as a model, as well as the examination of hydrolysis and aminolysis half-lives in different reaction conditions, these parameters being valuable in optimization of the use of the active esters...
May 15, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28238658/role-of-lotr1-in-nutrient-transport-through-organization-of-spatial-distribution-of-root-endodermal-barriers
#11
Baohai Li, Takehiro Kamiya, Lothar Kalmbach, Mutsumi Yamagami, Katsushi Yamaguchi, Shuji Shigenobu, Shinichiro Sawa, John M C Danku, David E Salt, Niko Geldner, Toru Fujiwara
The formation of Casparian strips and suberin lamellae at the endodermis limits the free diffusion of nutrients and harmful substances via the apoplastic space between the soil solution and the stele in roots [1-3]. Casparian strips are ring-like lignin polymers deposited in the middle of anticlinal cell walls between endodermal cells and fill the gap between them [4-6]. Suberin lamellae are glycerolipid polymers covering the endodermal cells and likely function as a barrier to limit transmembrane movement of apoplastic solutes into the endodermal cells [7, 8]...
March 6, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28164319/root-cortical-senescence-decreases-root-respiration-nutrient-content-and-radial-water-and-nutrient-transport-in-barley
#12
Hannah M Schneider, Tobias Wojciechowski, Johannes A Postma, Kathleen M Brown, Andreas Lücke, Viktoria Zeisler, Lukas Schreiber, Jonathan P Lynch
The functional implications of root cortical senescence (RCS) are poorly understood. We tested the hypotheses that RCS in barley: (1) reduces the respiration and nutrient content of root tissue; (2) decreases radial water and nutrient transport; (3) is accompanied by increased suberization to protect the stele. Genetic variation for RCS exists between modern germplasm and landraces. Nitrogen and phosphorus deficiency increased the rate of RCS. Maximal RCS, defined as the disappearance of the entire root cortex, reduced root nitrogen content by 66%, phosphorus content by 63%, and respiration by 87% compared to root segments with no RCS...
February 6, 2017: Plant, Cell & Environment
https://www.readbyqxmd.com/read/28149303/impact-of-leaf-traits-on-temporal-dynamics-of-transpired-oxygen-isotope-signatures-and-its-impact-on-atmospheric-vapor
#13
Maren Dubbert, Angelika Kübert, Christiane Werner
Oxygen isotope signatures of transpiration (δ E ) are powerful tracers of water movement from plant to global scale. However, a mechanistic understanding of how leaf morphological/physiological traits effect δ E is missing. A laser spectrometer was coupled to a leaf-level gas-exchange system to measure fluxes and isotopic signatures of plant transpiration under controlled conditions in seven distinct species (Fagus sylvatica, Pinus sylvestris, Acacia longifolia, Quercus suber, Coffea arabica, Plantago lanceolata, Oxalis triangularis)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28133655/kinetics-mechanisms-and-ionic-liquids-in-the-uptake-of-n-butylamine-onto-low-molecular-weight-dicarboxylic-acids
#14
Michelle C Fairhurst, Michael J Ezell, Carla Kidd, Pascale S J Lakey, Manabu Shiraiwa, Barbara J Finlayson-Pitts
Atmospheric particles adversely affect visibility, health, and climate, yet the kinetics and mechanisms of particle formation and growth are poorly understood. Multiphase reactions between amines and dicarboxylic acids (diacids) have been suggested to contribute. In this study, the reactions of n-butylamine (BA) with solid C3-C8 diacids were studied at 296 ± 1 K using a Knudsen cell interfaced to a quadrupole mass spectrometer. Uptake coefficients for amines on the diacids with known geometric surface areas were measured at initial amine concentrations from (3-50) × 10(11) cm(-3)...
January 30, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28065927/the-composite-water-and-solute-transport-of-barley-hordeum-vulgare-roots-effect-of-suberized-barriers
#15
Kosala Ranathunge, Yangmin X Kim, Friedrich Wassmann, Tino Kreszies, Viktoria Zeisler, Lukas Schreiber
Background and Aims: Roots have complex anatomical structures, and certain localized cell layers develop suberized apoplastic barriers. The size and tightness of these barriers depend on the growth conditions and on the age of the root. Such complex anatomical structures result in a composite water and solute transport in roots. Methods: Development of apoplastic barriers along barley seminal roots was detected using various staining methods, and the suberin amounts in the apical and basal zones were analysed using gas chromatography-mass spectometry (GC-MS)...
March 1, 2017: Annals of Botany
https://www.readbyqxmd.com/read/28063300/a-psma-targeted-theranostic-agent-for-photodynamic-therapy
#16
Ying Chen, Samit Chatterjee, Ala Lisok, Il Minn, Mrudula Pullambhatla, Bryan Wharram, Yuchuan Wang, Jiefu Jin, Zaver M Bhujwalla, Sridhar Nimmagadda, Ronnie C Mease, Martin G Pomper
Prostate-specific membrane antigen (PSMA) is over-expressed in the epithelium of prostate cancer and in the neovasculature of many non-prostate solid tumors. PSMA has been increasingly used as a target for cancer imaging and therapy. Here we describe a low-molecular-weight theranostic photosensitizer, YC-9, for PSMA-targeted optical imaging and photodynamic therapy (PDT). YC-9 was synthesized by conjugating IRDye700DX N-hydroxysuccinimide (NHS) ester with a PSMA targeting Lys-Glu urea through a lysine-suberate linker in suitable yield...
February 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28045988/differential-dna-methylation-patterns-are-related-to-phellogen-origin-and-quality-of-quercus-suber-cork
#17
Vera Inácio, Pedro M Barros, Augusta Costa, Cristóvão Roussado, Elsa Gonçalves, Rita Costa, José Graça, M Margarida Oliveira, Leonor Morais-Cecílio
DNA methylation is thought to influence Quercus suber cork quality, which is the main constraint for its economic valorisation. However, a deep knowledge of the cytosine methylation patterns disclosing the epigenetic variability of trees with different cork quality types is totally missing. This study investigates the hypothesis that variations in DNA methylation contribute to differences in cork cellular characteristics directly related to original or traumatic phellogen activity. We used MSAPs (Methylation Sensitive Amplified Polymorphism) to assess DNA methylation patterns of cork and leaf tissues of Q...
2017: PloS One
https://www.readbyqxmd.com/read/28004575/whole-milk-increases-intestinal-angptl4-expression-and-excretion-of-fatty-acids-through-feces-and-urine
#18
Søren Drud Nielsen, Bashar Amer, Karoline Blaabjerg, Trine K Dalsgaard, Randi Jessen, Bjørn Petrat-Melin, Martin Krøyer Rasmussen, Hanne D Poulsen, Jette F Young
The angiopoietin-like 4 (ANGPLT4) protein is involved in lipid metabolism and is known to inhibit lipoprotein lipase in the bloodstream. We investigated the effect of milk on intestinal ANGPTL4 and the metabolic profile of growing pigs and the effect of free fatty acids (FFAs) on ANGPTL4 in ex vivo and in vitro assays. Feeding pigs whole milk increased intestinal ANGPTL4 mRNA and increased fecal excretion of long-chain FFA compared to the control group fed soybean oil (n = 9). Furthermore, FFAs (C4-C8) induced ANGPTL4 gene expression in porcine intestinal tissue mounted in Ussing chambers and ANGPTL4 protein secretion to both the apical and basolateral sides of intestinal Caco-2 cells on permeable membranes...
January 18, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/27988001/novel-cellulose-based-amorphous-solid-dispersions-enhance-quercetin-solution-concentrations-in-vitro
#19
Andrew D Gilley, Hale Cigdem Arca, Brittany L B Nichols, Laura I Mosquera-Giraldo, Lynne S Taylor, Kevin J Edgar, Andrew P Neilson
Quercetin (Q) is a bioactive flavonol with potential to benefit human health. However, Q bioavailability is relatively low, due to its poor aqueous solubility and extensive phase-II metabolism. Strategies to increase solution concentrations in the small intestinal lumen have the potential to substantially increase Q bioavailability, and by extension, efficacy. We aimed to achieve this by incorporating Q into amorphous solid dispersions (ASDs) with cellulose derivatives. Q was dispersed in matrices of cellulose esters including 6-carboxycellulose acetate butyrate (CCAB), hydroxypropylmethylcellulose acetate succinate (HPMCAS) and cellulose acetate suberate (CASub) to afford ASDs that provided stability against crystallization, and pH-triggered release...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27986844/the-apoplasmic-pathway-via-the-root-apex-and-lateral-roots-contributes-to-cd-hyperaccumulation-in-the-hyperaccumulator-sedum-alfredii
#20
Qi Tao, Radek Jupa, Jipeng Luo, Alexander Lux, Ján Kováč, Yue Wen, Yimei Zhou, Japenga Jan, Yongchao Liang, Tingqiang Li
Although the significance of apoplasmic barriers in roots with regards to the uptake of toxic elements is generally known, the contribution of apoplasmic bypasses (ABs) to cadmium (Cd) hyperaccumulation is little understood. Here, we employed a combination of stable isotopic tracer techniques, an ABs tracer, hydraulic measurements, suberin lamellae staining, metabolic inhibitors, and antitranspirants to investigate and quantify the impact of the ABs on translocation of Cd to the xylem in roots of a hyperaccumulating (H) ecotype and a non-hyperaccumulating (NH) ecotype of Sedum alfredii In the H ecotype, the Cd content in the xylem sap was proportional to hydrostatic pressure, which was attributed to pressure-driven flow via the ABs...
December 16, 2016: Journal of Experimental Botany
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