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Kimberly A Lackey, Janet E Williams, William J Price, Janae M Carrothers, Sarah L Brooker, Bahman Shafii, Mark A McGuire, Michelle K McGuire
BACKGROUND: Inhibiting changes to bacteria in human milk between sample collection and analysis is necessary for unbiased characterization of the milk microbiome. Although cold storage is considered optimal, alternative preservation is sometimes necessary. RESEARCH AIM/QUESTION: The objective of this study was to compare the effectiveness of several commercially-available preservatives with regard to maintaining bacterial DNA in human milk for delayed microbiome analysis...
August 9, 2017: Journal of Microbiological Methods
Kathleen Deeley, Jacqueline Noel, Alexandre R Vieira
BACKGROUND: Obtaining DNA samples through saliva samples is becoming more common, and more companies are responding to this demand with saliva collection/DNA extraction kits. METHODS: The University of Pittsburgh's School of Dental Medicine maintains a DNA bank, which our lab helps direct. While we have always used Oragene (DNAGenotek, Kanata, Canada) kits to collect samples in the past, we recently compared 5 alternative kits/methods in an effort to reduce costs while maintaining quality...
September 1, 2016: Clinical Laboratory
Amy Sorensen, Elizabeth Rahman, Cassandra Canela, David Gangitano, Sheree Hughes-Stamm
One of the key features to be considered in a mass disaster is victim identification. However, the recovery and identification of human remains are sometimes complicated by harsh environmental conditions, limited facilities, loss of electricity and lack of refrigeration. If human remains cannot be collected, stored, or identified immediately, bodies decompose and DNA degrades making genotyping more difficult and ultimately decreasing DNA profiling success. In order to prevent further DNA damage and degradation after collection, tissue preservatives may be used...
November 2016: Forensic Science International. Genetics
Amy Sorensen, Clare Berry, David Bruce, Michelle Elizabeth Gahan, Sheree Hughes-Stamm, Dennis McNevin
Disaster victim identification (DVI) often occurs in remote locations with extremes of temperatures and humidities. Access to mortuary facilities and refrigeration are not always available. An effective and robust DNA sampling and preservation procedure would increase the probability of successful DNA profiling and allow faster repatriation of bodies and body parts. If the act of tissue preservation also released DNA into solution, ready for polymerase chain reaction (PCR), the DVI process could be further streamlined...
May 2016: International Journal of Legal Medicine
Malini Udtha, Rene Flores, Jennifer Sanner, Krystle Nomie, Elizabeth Backes, Luke Wilbers, James Caldwell
Preservation of biospecimens for biobanking applications traditionally involves freezing while maintaining the integrity of the product throughout multiple freeze-thaw cycles. The protection and stabilization of DNA at room temperature (RT) may eliminate the costs associated with freezer storage and reduce the maintenance costs for biobanks. However, there is a paucity of information describing the yield, purity, and integrity of DNA extracted from biospecimens stored at RT. To evaluate the yield, purity, and integrity of DNA extracted from whole blood samples stored at RT (18°C), low (-20°C), and ultra-low (-80°C) temperatures, whole blood samples from sheep and human subjects were collected, and aliquots were stored at RT (18°C), -20°C, and -80°C...
October 2014: Biopreservation and Biobanking
L M Wolfe, R D Thiagarajan, F Boscolo, V Taché, R L Coleman, J Kim, W K Kwan, J F Loring, M Parast, L C Laurent
INTRODUCTION: Banking of high-quality placental tissue specimens will enable biomarker discovery and molecular studies on diseases involving placental dysfunction. Systematic studies aimed at developing feasible standardized methodology for placental collection in a typical clinical setting are lacking. METHODS: To determine the acceptable timeframe for placental collection, we collected multiple samples from first and third trimester placentas at serial timepoints in a 2-h window after delivery, simultaneously comparing the traditional snap-freeze technique to commercial solutions designed to preserve RNA (RNAlater™), and DNA (DNAgard(®))...
August 2014: Placenta
Michael A Gray, Zoe A Pratte, Christina A Kellogg
Field collections of environmental samples, for example corals, for molecular microbial analyses present distinct challenges. The lack of laboratory facilities in remote locations is common, and preservation of microbial community DNA for later study is critical. A particular challenge is keeping samples frozen in transit. Five nucleic acid preservation methods that do not require cold storage were compared for effectiveness over time and ease of use. Mixed microbial communities of known composition were created and preserved by DNAgard(™), RNAlater(®), DMSO-EDTA-salt (DESS), FTA(®) cards, and FTA Elute(®) cards...
February 2013: FEMS Microbiology Ecology
A Allen-Hall, D McNevin
Disaster victim identification (DVI) poses unique challenges for forensic personnel. Typical scenarios may involve many bodies or body parts to identify in remote locations with limited access to laboratory facilities and in extreme temperatures. Transportation of tissue samples to a forensic laboratory for DNA profiling can take weeks without refrigeration. As well as protecting DNA for subsequent analysis, tissue preservation methods ideally should be safe, readily available and easy to transport to the scene at relatively low cost...
September 2012: Forensic Science International. Genetics
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