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https://www.readbyqxmd.com/read/25940193/evaluation-of-nucleic-acid-stabilization-products-for-ambient-temperature-shipping-and-storage-of-viral-rna-and-antibody-in-a-dried-whole-blood-format
#1
Allison L Dauner, Theron C Gilliland, Indrani Mitra, Subhamoy Pal, Amy C Morrison, Robert D Hontz, Shuenn-Jue L Wu
Loss of sample integrity during specimen transport can lead to false-negative diagnostic results. In an effort to improve upon the status quo, we used dengue as a model RNA virus to evaluate the stabilization of RNA and antibodies in three commercially available sample stabilization products: Whatman FTA Micro Cards (GE Healthcare Life Sciences, Pittsburgh, PA), DNAstāble Blood tubes (Biomātrica, San Diego, CA), and ViveST tubes (ViveBio, Alpharetta, GA). Both contrived and clinical dengue-positive specimens were stored on these products at ambient temperature or 37°C for up to 1 month...
July 2015: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/25340942/the-protection-and-stabilization-of-whole-blood-at-room-temperature
#2
Malini Udtha, Rene Flores, Jennifer Sanner, Krystle Nomie, Elizabeth Backes, Luke Wilbers, James Caldwell
Preservation of biospecimens for biobanking applications traditionally involves freezing while maintaining the integrity of the product throughout multiple freeze-thaw cycles. The protection and stabilization of DNA at room temperature (RT) may eliminate the costs associated with freezer storage and reduce the maintenance costs for biobanks. However, there is a paucity of information describing the yield, purity, and integrity of DNA extracted from biospecimens stored at RT. To evaluate the yield, purity, and integrity of DNA extracted from whole blood samples stored at RT (18°C), low (-20°C), and ultra-low (-80°C) temperatures, whole blood samples from sheep and human subjects were collected, and aliquots were stored at RT (18°C), -20°C, and -80°C...
October 2014: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/24315605/evaluation-of-dnastable-for-dna-storage-at-ambient-temperature
#3
Susanne E Howlett, Hilda S Castillo, Lora J Gioeni, James M Robertson, Joseph Donfack
Preserving DNA is important for validation of prospective and retrospective analyses, requiring many expensive types of equipment (e.g., freezers and back-up generators) and energy. While freezing is the most common method for storing extracted DNA evidence or well-characterized DNA samples for validation studies, DNAstable (Biomatrica), a commercially available medium for room temperature storage of DNA extracts was evaluated in this study. Two groups of samples consisting of different DNA quantities were investigated, one ranging from 20 to 400 ng (group 1) and the other one ranging from 1...
January 2014: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/23789643/protocols-for-dry-dna-storage-and-shipment-at-room-temperature
#4
COMPARATIVE STUDY
Natalia V Ivanova, Masha L Kuzmina
The globalization of DNA barcoding will require core analytical facilities to develop cost-effective, efficient protocols for the shipment and archival storage of DNA extracts and PCR products. We evaluated three dry-state DNA stabilization systems: commercial Biomatrica(®) DNAstable(®) plates, home-made trehalose and polyvinyl alcohol (PVA) plates on 96-well panels of insect DNA stored at 56 °C and at room temperature. Controls included unprotected samples that were stored dry at room temperature and at 56 °C, and diluted samples held at 4 °C and at -20 °C...
September 2013: Molecular Ecology Resources
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