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https://www.readbyqxmd.com/read/28928851/%C3%AE-transducin-repeat-containing-e3-ubiquitin-protein-ligase-inhibits-migration-invasion-and-proliferation-of-glioma-cells
#1
Jun Liang, Wei-Feng Wang, Shao Xie, Xian-Li Zhang, Wei-Feng Qi, Xiu-Ping Zhou, Jin-Xia Hu, Qiong Shi, Ru-Tong Yu
β-transducin repeat-containing E3 ubiquitin protein ligase (β-TrCP) serves as the substrate recognition subunit for the Skp1-Cullin1-F-box protein E3 ubiquitin ligase, which recognizes the double phosphorylated DSG (X)2+nS destruction motif in various substrates that are essential for numerous aspects of tumorigenesis and regulates several important signaling pathways. However, the biological significance of β-TrCP in glioma progression remains largely unknown. A previous study by the authors demonstrated that the levels of β-TrCP protein expression in brain glioma tissues were significantly lower compared with non-tumorous tissues and that higher grades of gliomas exhibited lower levels of β-TrCP expression in comparison with lower glioma grades...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28928220/characterization-of-a-cytoplasmic-glucosyltransferase-that-extends-the-core-trisaccharide-of-the-toxoplasma-skp1-e3-ubiquitin-ligase-subunit
#2
Kazi Rahman, Msano Mandalasi, Peng Zhao, M Osman Sheikh, Rahil Taujale, Hyun W Kim, Hanke van der Wel, Khushi Matta, Natarajan Kannan, John N Glushka, Lance Wells, Christopher M West
Skp1 is a subunit of the SCF (Skp1/Cullin-1/F-box protein) class of E3 ubiquitin ligases that are important for eukaryotic protein degradation. Unlike its animal counterparts, Skp1 from Toxoplasma gondii is hydroxylated by an O2-dependent prolyl-4-hydroxylase (PhyA), and the resulting hydroxyproline can subsequently be modified by a five-sugar chain. A similar modification is found in the social amoeba Dictyostelium, where it regulates SCF assembly and O2-dependent development. Homologous glycosyltransferases assemble a similar core trisaccharide in both organisms, and a bifunctional α-galactosyltransferase from CAZy family GT77 mediates addition of the final two sugars in Dictyostelium, generating Galα1,3Galα1,3Fucα1,2Galβ1,3GlcNAcα1-...
September 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28928219/o2-sensing-associated-glycosylation-exposes-the-f-box-combining-site-of-the-dictyostelium-skp1-subunit-in-e3-ubiquitin-ligases
#3
M Osman Sheikh, David Thieker, Gordon Chalmers, Christopher M Schafer, Mayumi Ishihara, Parastoo Azadi, Robert J Woods, John N Glushka, Brad Bendiak, James H Prestegard, Christopher M West
Skp1 is a conserved protein linking cullin-1 to F-box proteins in SCF (Skp1-Cullin1-F-box) E3 ubiquitin ligases, which modify protein substrates with polyubiquitin chains that typically target them for 26S proteasome-mediated degradation. In Dictyostelium (a social amoeba), Toxoplasma gondii (the agent for human toxoplasmosis), and other protists, Skp1 is regulated by a unique pentasaccharide attached to hydroxylated Pro-143 within its C-terminal F-box binding domain. Prolyl hydroxylation of Skp1 contributes to O2-dependent Dictyostelium development, but full glycosylation at that position is required for optimal O2 sensing...
September 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28905993/overexpression-of-f-box-only-protein-31-predicts-poor-prognosis-and-deregulates-p38%C3%AE-and-jnk-mediated-apoptosis-in-esophageal-squamous-cell-carcinoma
#4
Jia Liu, Liang Lv, Jian Gong, Yuyong Tan, Yun Zhu, Yinghuan Dai, Xin Pan, Michael S Y Huen, Bin Li, Sai Wah Tsao, Jirong Huo, Annie L M Cheung
F-box only protein 31 (FBXO31), a subunit of the Skp1-Cul1-F box ubiquitin ligase, plays a crucial role in DNA damage response and tumorigenesis. Yet its expression and function vary in different types of human cancer. The expression of FBXO31 in esophageal squamous cell carcinoma (ESCC) and its association with clinicopathological features is not well studied. The underlying mechanism by which deregulated FBXO31 contributes to ESCC tumorigenesis is largely unknown. By immunohistochemical analysis of a tissue microarray containing 85 cases of ESCC and matched adjacent non-cancerous tissue and an additional 10 cases of ESCC tissue samples, we found that FBXO31 was overexpressed in ESCC, and that its expression was significantly correlated with histological grade (P = 0...
September 14, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28883095/lps-modulates-p300-and-sirt1-to-promote-prmt1-stability-via-a-scf-fbxl17-recognized-acetyldegron
#5
Yandong Lai, Jin Li, Xiuying Li, Chunbin Zou
E3 ubiquitin ligase recognizes its protein substrates via specific molecular signatures for ubiquitin proteasomal degradation. However, the role of acetylation/ deacetylation in the process of E3 ubiquitin ligase recognizing its protein substrate is not fully studied. Here we report that a tandem IK motif in protein arginine methyltransferase 1 (PRMT1) forms an acetyldegron to recruit ubiquitin E3 ligase SCF (Skp1-Cullin1-Fbox protein)-Fbxl17. PRMT1 is poly-ubiquitinated for proteasome degradation with a half-life of approximate 4 h in lung epithelial cells...
September 7, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28871788/antifungal-effect-of-arabidopsis-sgt1-proteins-via-mitochondrial-reactive-oxygen-species
#6
Seong-Cheol Park, Mi Sun Cheong, Eun-Ji Kim, Jin Hyo Kim, Yong Hun Chi, Mi-Kyeong Jang
The highly conserved SGT1 (suppressor of the G2 alleles of skp1) proteins from Arabidopsis are known to contribute to plant resistance to pathogens. While SGT1 proteins response to fungal pathogens, their antifungal activity is not reported and the mechanism for this inhibition is not well understood. Therefore, recombinant Arabidopsis SGT1 proteins were cloned, expressed, and purified to evaluate their antifungal activity, resulted in their potent inhibition of pathogen growth. Dye-labeled proteins localized to the cytosol of Candida albicans cells without the disruption of the cell membrane...
September 5, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28818370/overexpression-of-vpeifp1-a-novel-f-box-kelch-repeat-protein-from-wild-chinese-vitis-pseudoreticulata-confers-higher-tolerance-to-powdery-mildew-by-inducing-thioredoxin-z-proteolysis
#7
Jie Wang, Wenkong Yao, Lei Wang, Fuli Ma, Weihuo Tong, Chen Wang, Rui Bao, Changyue Jiang, Yazhou Yang, Jianxia Zhang, Yan Xu, Xiping Wang, Chaohong Zhang, Yuejin Wang
An F-box protein (VpEIFP1) induced by Erysiphe necator was isolated from Vitis pseudoreticulata, a wild Chinese grapevine species naturally resistant to powdery mildew (PM). It contains an F-box domain and two Kelch-repeat motifs. Expression profiles indicate the VpEIFP1 is strongly induced at both transcriptional and translational levels by PM infection. A subcellular localisation assay showed that VpEIFP1 is predominantly located in the nucleus and cytoplasm. Overexpression of VpEIFP1 accelerated the accumulation of hydrogen peroxide (H2O2) and up-regulated the expressions of ICS2, NPR1 and PR1 involved in defence responses, resulting in suppression of PM germination and growth...
October 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28797197/dysregulated-expression-of-skp2-and-its-role-in-hematological-malignancies
#8
Michal Kulinski, Iman W Achkar, Mohammad Haris, Said Dermime, Ramzi R Mohammad, Shahab Uddin
S-phase kinase-associated protein 2 (SKP2) is a well-studied F-box protein and a critical part of the Skp1-Cul1-Fbox (SCF) E3 ligase complex. It controls cell cycle by regulating the expression level of p27 and p21 through ubiquitination and proteasomal degradation. SKP2-mediated loss of p27Kip1 is associated with poor clinical outcome in various types of cancers including hematological malignancies. It is however well established that SKP2 is an oncogene, and its targeting may be an attractive therapeutic strategy for the management of hematological malignancies...
August 10, 2017: Leukemia & Lymphoma
https://www.readbyqxmd.com/read/28767675/large-scale-identification-of-gossypium-hirsutum-genes-associated-with-verticillium-dahliae-by-comparative-transcriptomic-and-reverse-genetics-analysis
#9
Wenwei Zhang, Huachong Zhang, Kai Liu, Guiliang Jian, Fangjun Qi, Ning Si
Verticillium wilt is a devastating disease of cotton, which is caused by the soil-borne fungus Verticillium dahliae (V. dahliae). Although previous studies have identified some genes or biological processes involved in the interaction between cotton and V. dahliae, its underlying molecular mechanism remains unclear, especially in G. hirsutum. In the present study, we obtained an overview of transcriptome characteristics of resistant upland cotton (G. hirsutum) after V. dahliae infection at 24 h post-inoculation (hpi) via a high-throughput RNA-sequencing technique...
2017: PloS One
https://www.readbyqxmd.com/read/28743755/ubiquitination-of-exposed-glycoproteins-by-scf-fbxo27-directs-damaged-lysosomes-for-autophagy
#10
Yukiko Yoshida, Sayaka Yasuda, Toshiharu Fujita, Maho Hamasaki, Arisa Murakami, Junko Kawawaki, Kazuhiro Iwai, Yasushi Saeki, Tamotsu Yoshimori, Noriyuki Matsuda, Keiji Tanaka
Ubiquitination functions as a signal to recruit autophagic machinery to damaged organelles and induce their clearance. Here, we report the characterization of FBXO27, a glycoprotein-specific F-box protein that is part of the SCF (SKP1/CUL1/F-box protein) ubiquitin ligase complex, and demonstrate that SCF(FBXO27) ubiquitinates glycoproteins in damaged lysosomes to regulate autophagic machinery recruitment. Unlike F-box proteins in other SCF complexes, FBXO27 is subject to N-myristoylation, which localizes it to membranes, allowing it to accumulate rapidly around damaged lysosomes...
August 8, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28736168/light-dependent-degradation-of-pif3-by-scf-ebf1-2-promotes-a-photomorphogenic-response-in-arabidopsis
#11
Jie Dong, Weimin Ni, Renbo Yu, Xing Wang Deng, Haodong Chen, Ning Wei
Plant seedlings emerging from darkness into the light environment undergo photomorphogenesis, which enables autotrophic growth with optimized morphology and physiology. During this transition, plants must rapidly remove photomorphogenic repressors accumulated in the dark. Among them is PHYTOCHROME-INTERACTING FACTOR 3 (PIF3), a key transcription factor promoting hypocotyl growth. Here we report that, in response to light activation of phytochrome photoreceptors, EIN3-BINDING F BOX PROTEINs (EBFs) 1 and 2 mediate PIF3 protein degradation in a manner dependent on light-induced phosphorylation of PIF3...
August 21, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28722108/the-ubiquitin-ligase-scf-fbxw7%C3%AE-promotes-ata3-degradation
#12
Nan Song, Cheng Cao, Yiman Tang, Liyuan Bi, Yong Jiang, Yongsheng Zhou, Xin Song, Ling Liu, Wenshu Ge
GATA3 is a key transcription factor in cell fate determination and its dysregulation has been implicated in various types of malignancies. However, how the abundance and function of GATA3 are regulated remains unclear. Here, we report that GATA3 is physically associated with FBXW7α, and FBXW7α destabilizes GATA3 through assembly of a SKP1-CUL1-F-box E3 ligase complex. Importantly, we showed that FBXW7α promotes GATA3 ubiquitination and degradation in a GSK3 dependent manner. Furthermore, we demonstrated that FBXW7α inhibits breast cancer cells survival through destabilizing GATA3, and the expression level of FBXW7αis negatively correlated with that of GATA3 in breast cancer samples...
July 19, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28690630/lack-of-s-rnase-based-gametophytic-self-incompatibility-in-orchids-suggests-that-this-system-evolved-after-the-monocot-eudicot-split
#13
Shan-Ce Niu, Jie Huang, Yong-Qiang Zhang, Pei-Xing Li, Guo-Qiang Zhang, Qing Xu, Li-Jun Chen, Jie-Yu Wang, Yi-Bo Luo, Zhong-Jian Liu
Self-incompatibility (SI) is found in approximately 40% of flowering plant species and at least 100 families. Although orchids belong to the largest angiosperm family, only 10% of orchid species present SI and have gametophytic SI (GSI). Furthermore, a majority (72%) of Dendrobium species, which constitute one of the largest Orchidaceae genera, show SI and have GSI. However, nothing is known about the molecular mechanism of GSI. The S-determinants of GSI have been well characterized at the molecular level in Solanaceae, Rosaceae, and Plantaginaceae, which use an S-ribonuclease (S-RNase)-based system...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28652210/cyclin-f-a-component-of-an-e3-ubiquitin-ligase-complex-with-roles-in-neurodegeneration-and-cancer
#14
Jasmin Galper, Stephanie L Rayner, Alison L Hogan, Jennifer A Fifita, Albert Lee, Roger S Chung, Ian P Blair, Shu Yang
Cyclin F, encoded by CCNF, is the substrate recognition component of the Skp1-Cul1-F-box E3 ubiquitin ligase complex, SCF(cyclin F). E3 ubiquitin ligases play a key role in ubiquitin-proteasome mediated protein degradation, an essential component of protein homeostatic mechanisms within the cell. By recognising and regulating the availability of several protein substrates, SCF(cyclin F) plays a role in regulating various cellular processes including replication and repair of DNA and cell cycle checkpoint control...
August 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28619822/cop9-signalosome-subunits-protect-capicua-from-mapk-dependent-and-independent-mechanisms-of-degradation
#15
Annabelle Suisse, DanQing He, Kevin Legent, Jessica E Treisman
The COP9 signalosome removes Nedd8 modifications from the Cullin subunits of ubiquitin ligase complexes, reducing their activity. Here, we show that mutations in the Drosophila COP9 signalosome subunit 1b (CSN1b) gene increase the activity of ubiquitin ligases that contain Cullin 1. Analysis of CSN1b mutant phenotypes revealed a requirement for the COP9 signalosome to prevent ectopic expression of Epidermal growth factor receptor (EGFR) target genes. It does so by protecting Capicua, a transcriptional repressor of EGFR target genes, from EGFR pathway-dependent ubiquitylation by a Cullin 1/SKP1-related A/Archipelago E3 ligase and subsequent proteasomal degradation...
July 15, 2017: Development
https://www.readbyqxmd.com/read/28614300/pten-counteracts-fbxl2-to-promote-ip3r3-and-ca-2-mediated-apoptosis-limiting-tumour-growth
#16
Shafi Kuchay, Carlotta Giorgi, Daniele Simoneschi, Julia Pagan, Sonia Missiroli, Anita Saraf, Laurence Florens, Michael P Washburn, Ana Collazo-Lorduy, Mireia Castillo-Martin, Carlos Cordon-Cardo, Said M Sebti, Paolo Pinton, Michele Pagano
In response to environmental cues that promote IP3 (inositol 1,4,5-trisphosphate) generation, IP3 receptors (IP3Rs) located on the endoplasmic reticulum allow the 'quasisynaptical' feeding of calcium to the mitochondria to promote oxidative phosphorylation. However, persistent Ca(2+) release results in mitochondrial Ca(2+) overload and consequent apoptosis. Among the three mammalian IP3Rs, IP3R3 appears to be the major player in Ca(2+)-dependent apoptosis. Here we show that the F-box protein FBXL2 (the receptor subunit of one of 69 human SCF (SKP1, CUL1, F-box protein) ubiquitin ligase complexes) binds IP3R3 and targets it for ubiquitin-, p97- and proteasome-mediated degradation to limit Ca(2+) influx into mitochondria...
June 22, 2017: Nature
https://www.readbyqxmd.com/read/28609481/the-ubiquitin-conjugating-enzyme-cdc34-is-essential-for-cytokinesis-in-contrast-to-putative-subunits-of-a-scf-complex-in-trypanosoma-brucei
#17
Federico Rojas, Joanna Koszela, Jacqueline Búa, Briardo Llorente, Richard Burchmore, Manfred Auer, Jeremy C Mottram, María Teresa Téllez-Iñón
The ubiquitin-proteasome system is a post-translational regulatory pathway for controlling protein stability and activity that underlies many fundamental cellular processes, including cell cycle progression. Target proteins are tagged with ubiquitin molecules through the action of an enzymatic cascade composed of E1 ubiquitin activating enzymes, E2 ubiquitin conjugating enzymes, and E3 ubiquitin ligases. One of the E3 ligases known to be responsible for the ubiquitination of cell cycle regulators in eukaryotes is the SKP1-CUL1-F-box complex (SCFC)...
June 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28515316/dna-damage-induced-degradation-of-exo1-exonuclease-limits-dna-end-resection-to-ensure-accurate-dna-repair
#18
Nozomi Tomimatsu, Bipasha Mukherjee, Janelle Louise Harris, Francesca Ludovica Boffo, Molly Catherine Hardebeck, Patrick Ryan Potts, Kum Kum Khanna, Sandeep Burma
End resection of DNA double-strand breaks (DSBs) to generate 3'-single-stranded DNA facilitates DSB repair via error-free homologous recombination (HR) while stymieing repair by the error-prone non-homologous end joining (NHEJ) pathway. Activation of DNA end resection involves phosphorylation of the 5' to 3' exonuclease EXO1 by the phosphoinositide 3-kinase-like kinases ATM (ataxia telangiectasia-mutated) and ATR (ATM and Rad3-related) and by the cyclin-dependent kinases 1 and 2. After activation, EXO1 must also be restrained to prevent over-resection that is known to hamper optimal HR and trigger global genomic instability...
June 30, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28506875/deubiquitinase-usp33-is-negatively-regulated-by-%C3%AE-trcp-through-ubiquitin-dependent-proteolysis
#19
Qiao Cheng, Yukang Yuan, Lemin Li, Tingting Guo, Ying Miao, Ying Ren, Jin Liu, Qian Feng, Xiaofang Wang, Peng Zhao, Yibo Zuo, Liping Qian, Liting Zhang, Hui Zheng
Ubiquitin-mediated proteolysis regulates cellular levels of various proteins, and therefore plays important roles in controlling cell signaling and disease progression. The Skp1-Cul1-F-box ubiquitin ligase β-TrCP is recognized as an important negative regulator for numerous key signaling proteins. Recently, the deubiquitinases (DUBs) have turned out to be essential to regulate signaling pathways related to human diseases. However, whether β-TrCP is able to regulate the deubiquitinase family members remains largely unexplored...
July 1, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28494021/rice-black-streaked-dwarf-virus-p7-2-forms-a-scf-complex-through-binding-to-oryza-sativa-skp1-like-proteins-and-interacts-with-gid2-involved-in-the-gibberellin-pathway
#20
Tao Tao, Cui-Ji Zhou, Qian Wang, Xiang-Ru Chen, Qian Sun, Tian-Yu Zhao, Jian-Chun Ye, Ying Wang, Zong-Ying Zhang, Yong-Liang Zhang, Ze-Jian Guo, Xian-Bing Wang, Da-Wei Li, Jia-Lin Yu, Cheng-Gui Han
As a core subunit of the SCF complex that promotes protein degradation through the 26S proteasome, S-phase kinase-associated protein 1 (SKP1) plays important roles in multiple cellular processes in eukaryotes, including gibberellin (GA), jasmonate, ethylene, auxin and light responses. P7-2 encoded by Rice black streaked dwarf virus (RBSDV), a devastating viral pathogen that causes severe symptoms in infected plants, interacts with SKP1 from different plants. However, whether RBSDV P7-2 forms a SCF complex and targets host proteins is poorly understood...
2017: PloS One
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